CN105670958B - One plant of low temperature biocontrol bacterial strain and its application - Google Patents
One plant of low temperature biocontrol bacterial strain and its application Download PDFInfo
- Publication number
- CN105670958B CN105670958B CN201510762018.XA CN201510762018A CN105670958B CN 105670958 B CN105670958 B CN 105670958B CN 201510762018 A CN201510762018 A CN 201510762018A CN 105670958 B CN105670958 B CN 105670958B
- Authority
- CN
- China
- Prior art keywords
- bacterial strain
- low temperature
- biocontrol
- plant
- fungus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses one plant of low temperature biocontrol bacterial strain and its applications, the low temperature biocontrol bacterial strain is Methylotrophic bacillus (Bacillus methylotrophicus) WSWGH-10, it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.11541, the deposit date is on October 21st, 2015, preservation address was the institute 3 of Chaoyang District, Beijing City Beichen Lu 1.The biocontrol microorganisms can growth and breeding under cryogenic, can generate and bite a variety of antibacterial materials such as iron element, chitinase, lipopeptid class and protide, also there is degraded cellulose ability.The bacterial strain antimicrobial spectrum is wide, it is able to suppress the disease fungus such as pathogen of soybean root rot, soybean sclerotinia crown rot bacterium, Causal Organism of Maize Basal Stalk bacterium, fusarium moniliforme, Ustilaginoidea virens, rice wilt pathogens, julienne potatoes core germ, wilsonii pine root fungus, cucumber fusarium axysporum, botrytis cinerea and pathogenetic bacteria (tobacco bacterial disease angular leaf spot fungus), but also has the function of to promote plant growth.
Description
Technical field
The invention belongs to microorganisms technical fields, are related to one plant of low temperature biocontrol bacterial strain and its application.
Background technique
Corps diseases are a kind of natural calamities in agricultural production, directly affect the yield and quality of crop, caused by
It loses very huge.Due to getting worse for chemical pesticide drug resistance and pollution problem, environmental-friendly microbial pesticide becomes
The important means of crop disease control.Currently, microbial pesticide, based on active bacteria formulation, it is general character that preventive effect is unstable in
Problem.It is the key that influence its practical protection effect that can biocontrol microorganisms, which survive and breed in soil environment,.It is broadcast due to common
The kind time is in the soil moisture lower early spring or autumn, and microbial pesticide bacterial strain used is room temperature bacterium now, in low temperature
Under the conditions of slow growth or stop growing, reduce the application effect of biological pesticide, being widely popularized for microbial pesticide hindered to answer
With, therefore the acquisition of low temperature biocontrol bacterial strain can provide good bacterium source for the bio-bacterial manure of low temperature adaptability and the research and development of biological pesticide
Material has potential research and exploitation advantages in biological control.
Summary of the invention
The object of the present invention is to provide one plant of low temperature biocontrol bacterial strain, the biocontrol microorganisms can growth and breeding under cryogenic,
It can generate and bite a variety of antibacterial materials such as iron element, chitinase, lipopeptid class and protide, also there is degraded cellulose ability.It should
Bacterial strain antimicrobial spectrum is wide, is able to suppress pathogen of soybean root rot, soybean sclerotinia crown rot bacterium, Causal Organism of Maize Basal Stalk bacterium, fusarium moniliforme, water
Rice ustilaginoidea virens, rice wilt pathogens, julienne potatoes core germ, wilsonii pine root fungus, cucumber fusarium axysporum, graw mold of tomato
The disease fungus such as bacterium and pathogenetic bacteria (tobacco bacterial disease angular leaf spot fungus), but also have the function of to promote plant growth.
The purpose of the present invention is what is be achieved through the following technical solutions:
One plant of low temperature biocontrol bacterial strain, for Methylotrophic bacillus (Bacillus methylotrophicus)
WSWGH-10 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCC
No.11541, the deposit date is on October 21st, 2015, preservation address was the institute 3 of Chaoyang District, Beijing City Beichen Lu 1.
Methylotrophic bacillus (Bacillus methylotrophicus) WSWGH-10 morphological character: gram
Positive aerobic bacterium, it is rod-shaped, there is gemma, size is 0.2~0.5 × 1.0~1.5 μm;Bacteria colony white is round, gauffer.
Methylotrophic bacillus (Bacillus methylotrophicus) WSWGH-10 physio-biochemical characteristics: benefit
With glucose, oxidizing ferment, catalase, M.R, gelatin liquefaction, Starch Hydrolysis, citrate utilization, nitrate reduction, indoles are generated
It is the positive, V.P and urease-negative with litmus milk;6~30 DEG C of growth temperature range, 20 DEG C of optimum growth temperature.
Methylotrophic bacillus (Bacillus methylotrophicus) WSWGH-10 molecular biology identification knot
Fruit: being 99% with Methylotrophic bacillus homology by 16S rDNA sequence and house-keeping gene gyrB sequence alignment.
Methylotrophic bacillus (Bacillus methylotrophicus) WSWGH-10 of the invention can be generated
A variety of antibacterial materials such as iron element, chitinase, lipopeptid class and protide are bitten, also there is degraded cellulose ability.
Methylotrophic bacillus (Bacillus methylotrophicus) WSWGH-10 of the invention can be low
10 DEG C of warm condition effectively inhibits pathogen of soybean root rot, soybean sclerotinia crown rot bacterium, Causal Organism of Maize Basal Stalk bacterium, fusarium moniliforme, rice
Ustilaginoidea virens, rice wilt pathogens, julienne potatoes core germ, wilsonii pine root fungus, cucumber fusarium axysporum, botrytis cinerea
The growth of equal disease fungus and pathogenetic bacteria (tobacco bacterial disease angular leaf spot fungus), inhibiting rate are greater than 82%, and antimicrobial spectrum is wide,
Bacteriostasis rate is high.
Methylotrophic bacillus (Bacillus methylotrophicus) WSWGH-10 of the invention, which has, to be promoted
Plant growth function, 50 times and 100 times of dilutions can promote corn seed germination, and germination percentage is 90.3% and 94.5%,
Greater than blank control (88.2%), and bud is long, root long and radical are noticeably greater than blank control.
Preservation information:
Classification naming: Methylotrophic bacillus (Bacillus methylotrophicus);
Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center;
Deposit number: CGMCC No.11541;
Preservation date: on October 21st, 2015;
Preservation address: the institute 3 of Chaoyang District, Beijing City Beichen Lu 1.
Detailed description of the invention
Fig. 1 is thalli morphology;
Fig. 2 is colonial morphology;
Fig. 3 is bacterial strain 16s rRNA systematic evolution tree;
Fig. 4 is bacterial strain gyrB phylogenetic analysis;
Fig. 5 is that strain secretes bite the detection of iron element ability;
Fig. 6 is the detection of strain secretes cellulase ability;
Fig. 7 is the detection of strain secretes chitinase ability;
Fig. 8 is the detection of strain secretes protease ability;
Fig. 9 is antibacterial protein Antibacterial Activity;
Figure 10 is lipopeptide antibiotic Antibacterial Activity;
Figure 11 is suppression result of the bacterial strain to Tobacco Angular Leaf Spot Disease bacterium;
Figure 12 is that bacterial strain promotes corn seed germination result.
Specific embodiment
Further description of the technical solution of the present invention with reference to the accompanying drawing, and however, it is not limited to this, all to this
Inventive technique scheme is modified or replaced equivalently, and without departing from the spirit and scope of the technical solution of the present invention, should all be covered
Within the protection scope of the present invention.
The present invention provides one plant of low temperature biocontrol bacterial strain and its application, particular content are as follows:
1) screening and identification of low temperature biocontrol bacterial strain:
Low temperature biocontrol bacterial strain WSWGH-10 is that separation screening obtains from the winter frozen soil of Heilongjiang Province Hegang District.Screening is pressed
Following methods carry out: grinding, be put into the triangular flask containing 50ml sterile water, 10 DEG C of concussion 3h, with nothing after taking 5g frozen soil to thaw
Bacterium water is diluted to 10-7, 10 are taken respectively-5、10-6With 10-7The bacterium solution 0.1ml of degree of rarefication is coated on LB plate, in 10 DEG C of incubators
3~7d of stationary culture chooses the new LB plate of single colonie scribing line switching, 3~7d of stationary culture in 10 DEG C of incubators.With soybean root rot
Germ is indicator bacteria, screens biocontrol bacterial strain using opposite culture method, can be obtained low temperature biocontrol bacterial strain.
Form, cultural character and physio-biochemical characteristics identification are carried out to bacterial strain, the specific steps are as follows: Low- temperature culture 24 is small
When thallus carry out Gram's staining after under 400 power microscopes observe thallus size;LB will be coated on after bacterium solution doubling dilution
On culture medium flat plate, colonial morphology and color are observed;Bacterial strain is subjected to oxidizing ferment, catalase, glucose fermentation, V.P, M.R form sediment
Powder hydrolysis, urease, citrate utilization, nitrate reduction, indoles generates, gelatin liquefaction and litmus milk are tested, as a result table
The bright bacterial strain is Gram positive aerobic bacterium, rod-shaped, there is gemma, and size is 0.2~0.5 × 1.0~1.5 μm (Fig. 1);Bacterium colony is white
Color is round, gauffer (Fig. 2);Glucose can be utilized;Oxidizing ferment, catalase, M.R, gelatin liquefaction, Starch Hydrolysis, citrate
Using, nitrate reduction, indoles generate and litmus milk be the positive, V.P and urease-negative.Growth temperature range 6~30
DEG C, 20 DEG C of optimum growth temperature.
Carry out the analysis of 16S rDNA sequence and house-keeping gene gyrB analysis to bacterial strain: taking 1mL bacterium solution, thalline were collected by centrifugation, uses
Bacterial genomes DNA extraction kit (TIANGEN BIOTECH) extracts genomic DNA, using genomic DNA as template, respectively
PCR amplification is carried out with 16SrDNA universal primer and bacillus gyrB gene universal primer, PCR product progress agarose is coagulated
Specific DNA band is sequenced gel electrophoresis, homologous sequence comparison is carried out in GenBank, with 5.0 software building system of Mega
(Fig. 3~4) are set in system development, are as a result with the 16S rDNA sequence of Methylotrophic bacillus and gyrB genetic homology
99%, combining form, cultural character and physio-biochemical characteristics are accredited as Methylotrophic bacillus
methylotrophicus。
2) strain secretes protease, cellulase, chitinase and bite iron element ability detection:
Using protease, cellulase, chitinase and bite iron element identify flat band method detect whether the bacterial strain can generate
Protease, cellulase, chitinase and bite iron element.Protease detection: strain inoculated is detected into culture medium flat plate in protease
Upper (skimmed milk power 1%, beef extract 0.3%, peptone 1%, sodium chloride 0.5%), 10 DEG C of 3~7d of culture, whether there is or not enzymatic hydrolysis for observation
Transparent circle.Cellulase detection: by strain inoculated on Cellulose and congo red differential medium (ammonium sulfate 0.2%, potassium dihydrogen phosphate
0.1%, sodium chloride 0.05%, cellulose powder 2%, Congo red 0.02%, agar powder 2%), 10 DEG C of 3~7d of culture, observation whether there is or not
Enzymatic hydrolysis circle.Chitinase detection: by strain inoculated in chitin culture (tobacco brown spot pathogen 1%, peptone 0.2%, yeast powder
0.2%, magnesium sulfate 0.02%, 0.001% dipotassium hydrogen phosphate 0.07% of ferric sulfate, potassium dihydrogen phosphate 0.03% is Congo red
0.02%, agar powder 2%) 10 DEG C of 3~7d of culture, whether there is or not enzymatic hydrolysis to enclose for observation.Bite the detection of iron element: by strain inoculated CAS- plate,
10 DEG C of 3~7d of culture, whether there is or not enzymatic hydrolysis to enclose for observation.The result shows that the bacterial strain can extracellular proteinase, cellulase, chitinase and
Iron element is bitten, illustrates the bacterial strain not only and have the function of biological and ecological methods to prevent plant disease, pests, and erosion also that there is the ability (Fig. 5-8) of degraded cellulose and albumen.
3) antibacterial material of low temperature biocontrol microorganisms determines:
Antibacterial protein: by bacterial strain 10 DEG C in LB culture medium, 170r/min culture 3d, 4000r/mim centrifugation, supernatant is collected
Liquid obtains albumen using ammonium sulfate precipitation method, and first plus ammonium sulfate is to 30% saturation degree, 4 DEG C of overnight precipitations, 10000r/
Mim is centrifuged 20min, collects precipitating, must precipitate I.Supernatant adds ammonium sulfate to 50%, 70% and 90% saturation degree, 4 DEG C of mistakes
Night precipitating, 10000r/mim are centrifuged 20min, collect precipitating II, III and IV, are dissolved with PBS buffer solution, desalination of dialysing, after concentration
Bacteriostatic activity is detected by indicator bacteria of pathogen of soybean root rot, the results showed that precipitating II, III and IV have bacteriostatic activity (Fig. 9),
Illustrate that the bacterial strain can generate a variety of antibacterial proteins.
Lipopeptide antibiotic: it by bacterial strain 10 DEG C in LB culture medium, 170r/min culture 3d, 4000r/mim centrifugation, collects
Supernatant obtains lipopeptide antibiotic using hydrochloric acid precipitation method.Concentrated hydrochloric acid is added into supernatant to stand overnight to 2,4 DEG C of pH,
Next day 10000r/mim is centrifuged 20min, collects precipitating, is extracted with methanol, use paper disk method using pathogen of soybean root rot as indicator bacteria
Bacteriostatic activity is detected, as a result lipopeptide antibiotic has bacteriostatic activity (Figure 10), and it is anti-to illustrate that the bacterial strain can also generate lipopeptid class
Raw element.
4) the antimicrobial spectrum measurement of low temperature biocontrol microorganisms:
It is (pathogen of soybean root rot, big to 10 kinds of plant pathogenic fungis using mycelial growth rate method measurement bacterial strain WSWHG-10
Beans hyphal cluster germ, Causal Organism of Maize Basal Stalk bacterium, fusarium moniliforme, Ustilaginoidea virens, rice wilt pathogens, julienne potatoes core disease
Bacterium, wilsonii pine root fungus, cucumber fusarium axysporum, botrytis cinerea) inhibit situation.0.1ml Low- temperature culture 48h bacterium solution is taken to apply
It is distributed on PDA plate, is inoculated with pathogen mycelia block (diameter 5mm) in plate center, coating sterile water compares, 10 DEG C of cultures
7d measures colony diameter, calculates bacteriostasis rate as follows.Bacteriostasis rate %=control group colony diameter-control group colony diameter/
Control group colony diameter Х 100%.The result shows that the bacterial strain has good inhibitory effect to 10 kinds of disease fungus, inhibiting rate reaches
82.0~90.3% (tables 1) illustrate that the bacterial strain antimicrobial spectrum is wide, and bacteriostasis is strong.
Antibacterial result of 1 bacterial strain of table to disease fungus
Using inhibition zone method measurement bacterial strain WSWHG-10 to the pathogen (angular leaf spot fungus) for causing tobacco bacterial disease
Inhibit situation, take 0.1ml pathogenetic bacteria to be coated on LB plate, put sterilizing steel ring at center, it is raw that 0.05ml is added in steel ring
Anti- bacterium solution, 10 DEG C of culture 7d, whether there is or not inhibition zones for observation, as a result have inhibition zone around steel ring, illustrate biocontrol microorganisms WSWHG-10 energy
The pathogenetic bacteria is enough inhibited to grow (Figure 11).These results suggest that the bacterial strain antimicrobial spectrum is wide, not only to various plants disease fungus
With very strong inhibiting effect, also there is good inhibiting effect to bacterial disease.
5) the growth promotion measurement of low temperature biocontrol microorganisms:
The bacterium solution sterile water for cultivating 48h is diluted 50 and 100 times, corn seed is immersed in 4d in 2 treatment fluids, is taken
It is put on aqueous filter paper out, 28 DEG C of moisturizing culture 3d, calculates germination percentage, be control with clear water processing.The result shows that 50 and 100 times
Dilution promotes germination, and germination percentage is 90.3% and 94.5%, is greater than blank control (88.2%), and bud is long, root long
Blank control (table 2, Figure 12) is noticeably greater than with radical.
Influence of 2 bacterial strain of table to corn seed germination
Claims (5)
1. one plant of low temperature biocontrol bacterial strain, classification naming is Methylotrophic bacillus (Bacillus
Methylotrophicus), deposit number is CGMCC No. 11541.
2. low temperature biocontrol bacterial strain described in claim 1 inhibits plant pathogenic fungi and tobacco bacterial disease angle in cryogenic conditions
Application in spot pathogen growth, the plant pathogenic fungi are pathogen of soybean root rot, soybean sclerotinia crown rot bacterium, Causal Organism of Maize Basal Stalk
Bacterium, fusarium moniliforme, Ustilaginoidea virens, rice wilt pathogens, julienne potatoes core germ, wilsonii pine root fungus, cucumber
Wilt, botrytis cinerea.
3. low temperature biocontrol bacterial strain according to claim 2 inhibits plant pathogenic fungi and tobacco bacillary in cryogenic conditions
Application in the growth of disease angular leaf spot fungus, it is characterised in that the cryogenic temperature is 10 DEG C.
4. low temperature biocontrol bacterial strain described in claim 1 is promoting the application in plant growth.
5. application of the low temperature biocontrol bacterial strain in degraded cellulose and albumen described in claim 1.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510762018.XA CN105670958B (en) | 2015-11-10 | 2015-11-10 | One plant of low temperature biocontrol bacterial strain and its application |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510762018.XA CN105670958B (en) | 2015-11-10 | 2015-11-10 | One plant of low temperature biocontrol bacterial strain and its application |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105670958A CN105670958A (en) | 2016-06-15 |
CN105670958B true CN105670958B (en) | 2018-12-25 |
Family
ID=56947075
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510762018.XA Active CN105670958B (en) | 2015-11-10 | 2015-11-10 | One plant of low temperature biocontrol bacterial strain and its application |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105670958B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113142214A (en) * | 2021-05-24 | 2021-07-23 | 黑龙江省科学院微生物研究所 | Application of antibacterial protein of bacillus methylotrophicus wswGH-10 and separation and purification method |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106367375B (en) * | 2016-09-26 | 2019-02-12 | 黑龙江省科学院微生物研究所 | One plant of interior raw biocontrol bacterial strain for inhibiting wilsonii damping-off and its application |
CN106957805B (en) * | 2017-01-21 | 2020-10-23 | 南京钱塘洪泰生物科技有限公司 | Bacillus GBacilus-9 strain with bacteriostatic effect and separation method and application thereof |
CN108441458B (en) * | 2017-11-22 | 2021-05-25 | 东北农业大学 | Genetically engineered bacillus amyloliquefaciens capable of effectively preventing and treating gray mold as well as preparation method and application thereof |
CN108085269B (en) * | 2017-11-23 | 2019-12-06 | 中国农业科学院麻类研究所 | Bacillus methylotrophicus strain IBFCBF-2 and application thereof |
CN107760631B (en) * | 2017-11-24 | 2021-04-09 | 四川农业大学 | Bacillus methylotrophicus B18, and granules and application thereof |
CN108238681B (en) * | 2017-12-22 | 2021-07-20 | 江苏世邦生物工程科技有限公司 | Composite biological agent for low-temperature sewage treatment and preparation method and application thereof |
CN108641989B (en) * | 2018-06-28 | 2021-06-15 | 山东农业大学 | Bacillus methylotrophicus and application thereof |
CN110452852B (en) * | 2019-08-26 | 2021-09-28 | 浙江省农业科学院 | Farmer market biomass waste fermentation inoculant and preparation method thereof |
CN110526760A (en) * | 2019-10-09 | 2019-12-03 | 吉林省农业科学院 | A kind of compound amino acid fertilizer and its application method improving nursery stage rice plant cold resistance |
CN111517863B (en) * | 2020-05-09 | 2022-02-08 | 山东农业大学 | Bio-organic fertilizer containing bacillus methylotrophicus and preparation method thereof |
CN114946484B (en) * | 2022-04-21 | 2023-04-07 | 辽宁省农业科学院 | Application of bacillus methylotrophicus CB-12 in prevention and treatment of rice false smut |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101760439A (en) * | 2009-11-19 | 2010-06-30 | 无锡亚克生物科技有限公司 | Bacillus pumilus NMCC 46 and application thereof |
CN101928680A (en) * | 2009-11-19 | 2010-12-29 | 无锡亚克生物科技有限公司 | Bacillus simplex RJGP41 and application thereof |
CN101928681A (en) * | 2009-11-19 | 2010-12-29 | 无锡亚克生物科技有限公司 | Bacillus pumilus NMCN1 and application thereof |
WO2014066903A1 (en) * | 2012-10-26 | 2014-05-01 | Bayer Cropscience Lp, A Delawre Limited Partnership | Biocontrol of nematodes |
-
2015
- 2015-11-10 CN CN201510762018.XA patent/CN105670958B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101760439A (en) * | 2009-11-19 | 2010-06-30 | 无锡亚克生物科技有限公司 | Bacillus pumilus NMCC 46 and application thereof |
CN101928680A (en) * | 2009-11-19 | 2010-12-29 | 无锡亚克生物科技有限公司 | Bacillus simplex RJGP41 and application thereof |
CN101928681A (en) * | 2009-11-19 | 2010-12-29 | 无锡亚克生物科技有限公司 | Bacillus pumilus NMCN1 and application thereof |
WO2014066903A1 (en) * | 2012-10-26 | 2014-05-01 | Bayer Cropscience Lp, A Delawre Limited Partnership | Biocontrol of nematodes |
Non-Patent Citations (4)
Title |
---|
The Biocontrol Effects of the Bacillus licheniformis W10 Strain and Its Antifungal Protein Against Brown Rot in Peach;JI Zhaolin;《Horticultural Plant Journal》;20151130;第1卷(第3期);第131-138页 * |
冻土荒漠区分离低温适生PGPR菌的鉴定及其抗菌促生特性;谢永丽;《中国生物防治学报》;20140208;第30卷(第1期);第94-100页 * |
西藏低温适生芽孢杆菌的分离鉴定及其抗菌和促生作用;刘芳;《中国生物防治》;20101108;第26卷(第4期);第453-460页 * |
高寒草甸根围拮抗芽孢杆菌筛选鉴定及脂肽化合物分析;谢永丽;《中国生物防治学报》;20120829;第28卷(第3期);第367-374 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113142214A (en) * | 2021-05-24 | 2021-07-23 | 黑龙江省科学院微生物研究所 | Application of antibacterial protein of bacillus methylotrophicus wswGH-10 and separation and purification method |
CN113142214B (en) * | 2021-05-24 | 2022-05-03 | 黑龙江省科学院微生物研究所 | Application of antibacterial protein of bacillus methylotrophicus wswGH-10 and separation and purification method |
Also Published As
Publication number | Publication date |
---|---|
CN105670958A (en) | 2016-06-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105670958B (en) | One plant of low temperature biocontrol bacterial strain and its application | |
CN104498386B (en) | The preparation method and application of raw Bacillus amyloliquefaciens strain SZ23 and zymotic fluid in wild jujube | |
CN104342394B (en) | One plant of Siam bacillus and its application in terms of preventing and treating Fusarium graminearum | |
CN103173397A (en) | Broad-spectrum antibacterial bacillus amyloliquefaciens strain and application thereof | |
CN113969247B (en) | Bacterium for inhibiting tobacco disease pathogenic bacteria and application thereof | |
CN105441366B (en) | Methylotrophic bacillus ZBL-1 is applied in preventing cotton verticillium wilt | |
CN113528370B (en) | Bacillus belgii strain and application thereof | |
CN104531559A (en) | Bacillusamyloliquefaciens subsp Lh-1 and application thereof | |
CN107475134B (en) | One plant of Fusarium solani and its application in prevention and treatment dendrobium candidum southern blight | |
CN105462881B (en) | A kind of Paenibacillus polymyxa and its application for being used to prevent crop verticillium wilt | |
CN104651260A (en) | Brevibacillus brevis BBC-3 and application thereof as well as preparation method of microbial inoculum of brevibacillus brevis | |
CN103333810B (en) | Serratia marcescens and application thereof | |
CN105316266A (en) | Bacillus amyloliquefaciens LX-J1 and application thereof | |
CN102174431A (en) | Bio-control strain for preventing and controlling bacterial fruit blotch of watermelon | |
CN113444651B (en) | Saffron endophytic fungus and application thereof in preventing and treating bulb rot | |
CN105132294B (en) | A kind of alternaric bacteria and its application in dendrobium candidum disease-resistanting leaf spot | |
CN104195050A (en) | Lecanicillium attenuatum and application of lecanicillium attenuatum to control of crop nematodes and bemisia tabaci | |
CN108102944B (en) | Actinomycetes and application thereof | |
CN106035378A (en) | Compound bacterium agent for preventing and treating bacterial diseases of tobacco | |
CN111280183B (en) | Apple anthracnose leaf blight biocontrol microbial inoculum and preparation method and application thereof | |
CN105176885A (en) | Bacillus subtilis and application of bacillus subtilis in degrading organophosphorus pesticide | |
CN105368739A (en) | Bacillus amyloliquefaciens strain capable of resisting fruit tree pathogenic fungi in broad spectrum and application thereof | |
CN105462882B (en) | A kind of pseudomonas aeruginosa and its application for preventing crop verticillium wilt | |
CN102154155B (en) | Brevibacillus brevis for preventing and treating plant fungus diseases and method for preparing biopesticide | |
CN115537363B (en) | Bacillus subtilis, microbial inoculum, biological agent and application thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |