CN105651394B - A kind of nanoscale three-dimensional quantum thermal imaging system - Google Patents
A kind of nanoscale three-dimensional quantum thermal imaging system Download PDFInfo
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- CN105651394B CN105651394B CN201610157837.6A CN201610157837A CN105651394B CN 105651394 B CN105651394 B CN 105651394B CN 201610157837 A CN201610157837 A CN 201610157837A CN 105651394 B CN105651394 B CN 105651394B
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- lens
- laser
- modulator
- phase
- reflecting
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- 238000001931 thermography Methods 0.000 title claims abstract description 8
- 239000002096 quantum dot Substances 0.000 claims abstract description 24
- 230000003287 optical effect Effects 0.000 claims abstract description 22
- 238000001228 spectrum Methods 0.000 claims abstract description 12
- 241000931526 Acer campestre Species 0.000 claims abstract description 5
- 239000004973 liquid crystal related substance Substances 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 5
- 230000005540 biological transmission Effects 0.000 claims description 4
- 230000005526 G1 to G0 transition Effects 0.000 claims description 3
- 230000003321 amplification Effects 0.000 claims description 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
- 239000000571 coke Substances 0.000 claims description 2
- 238000009529 body temperature measurement Methods 0.000 abstract description 3
- 238000005259 measurement Methods 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 10
- 238000012512 characterization method Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 101100117236 Drosophila melanogaster speck gene Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J5/00—Radiation pyrometry, e.g. infrared or optical thermometry
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
Abstract
A kind of nanoscale three-dimensional quantum thermal imaging system, including laser, it is provided in its optical path and is placed with the objective table containing quantum dot sample, laser irradiation sample, amplify through object lens and is imaged, it is divided into reflection through half-reflecting half mirror again, transmitted light path, the reflected light of half-reflecting half mirror filters out the inconsistent light of the optical maser wavelength issued with laser through the first filter plate, the image position reflected through half-reflecting half mirror is in the front focal plane of the first lens, phase-modulator is located at the back focal plane of the first lens, phase-modulator is located at the front focal plane of the second lens, CCD is located at the back focal plane of the second lens, phase-modulator, CCD is connect with computer, reflecting mirror is provided on transmitted light path, the second filter plate and optical spectrum instrumentation are provided on the reflected light path of reflecting mirror, second filter plate filters out light identical with the optical maser wavelength of laser sending, spectrum Measuring instrument is connect with computer, and the positioning of quantum dot and temperature measurement are combined, nanoscale three-dimensional temperature field measurement is realized.
Description
Technical field
The invention belongs to micro-nano Three Dimensional Thermal technical field of imaging, and in particular to a kind of nanoscale three-dimensional quantum thermal imaging system.
Background technique
In field of biology, as the basic place of metabolism, intracellular temperature field is able to reflect various components
Thermodynamic behaviour and function and state change to entire biological phenomena.Pass through heat or temperature in research cell metabolism procedure
The variation of degree can further recognize vital activity phenomenon, changing rule and influence factor.The method of temperature measurement at present has
Tens of kinds, wherein common are alcohol thermometer, thermocouple thermometer and infra-red thermometer etc..Under macro-scale, they
Preferable effect can be obtained greatly.However, it is small by cell volume, heat production intensity is low etc., and factors are influenced, traditional calorimetric technology
But it is difficult to obtain satisfied result.
Quantum dot provides the physical base of cell thermometric as a kind of novel marking tool, Photothermal characterisation for people
Plinth.However, since its is small in size, fluorescence intensity is weak, when high-precision real of quantum dot, has been positioned to bottleneck.Common copolymerization coke is swept
Although retouching microscope, tomoscan microscope can position quantum dot, require to Sample Scan, in some realities
The more demanding occasion of when property, they are simultaneously not suitable for.So the average temperature studied all just for cell entirety many so far
Degree.Currently, biologist and physicians are limited to the micro-nano of the heat distribution of cell level on the road of research biological phenomena
Grade research.
Summary of the invention
In order to overcome the disadvantages of the above prior art, the purpose of the present invention is to provide a kind of nanoscale three-dimensional quantum thermal imagerys
Instrument realizes the measurement in nanoscale three-dimensional temperature field under the requirement for meeting accuracy and sensitivity.
In order to achieve the above object, the technical solution adopted by the present invention is that:
A kind of nanoscale three-dimensional quantum thermal imaging system, including laser 1 are successively set on the laser optical path that laser 1 issues
It is equipped with objective table 3, object lens 4 and half-reflecting half mirror 5, the sample containing several quantum dots 2 is placed on objective table 3, laser 1 is sent out
On sample, sample and quantum dot 2 are imaged laser irradiation through the amplification of object lens 4, then are divided into two-way light through the effect of half-reflecting half mirror 5 out
Road is all the way reflected light path, is all the way transmitted light path.
Be disposed on the reflected light path of half-reflecting half mirror 5 first filter plate 6, the first lens 7, phase-modulator 8,
The reflected light of second lens 9, CCD10, half-reflecting half mirror 5 filters out the laser wave issued with laser 1 through the first filter plate 6
Long inconsistent light reflects obtained image position in the front focal plane of the first lens 7 through half-reflecting half mirror 5, and phase-modulator 8 is located at the
The back focal plane of one lens 7, phase-modulator 8 also are located at the front focal plane of the second lens 9, phase-modulator 8 and computer 14 simultaneously
Connection, CCD10 are located at the back focal plane of the second lens 9, and CCD10 is connect with computer 14.
It is provided with reflecting mirror 11 on the transmitted light path of half-reflecting half mirror 5, is provided on the reflected light path of reflecting mirror 11
Second filter plate 12 and optical spectrum instrumentation 13, the second filter plate 12 filter out light identical with the optical maser wavelength of the sending of laser 1,
Optical spectrum instrumentation 13 is connect with computer 14.
The phase-modulator 8 is stationary phase plate, or is transmission liquid crystal phase-modulator, or is reflection type liquid crystal
Phase-modulator.
Advantages of the present invention: it regard quantum dot 2 as thermal probe, is based on its Photothermal characterisation, obtains it with spectral measurement method
Environment temperature, at the same use space light modulating method realize quantum dot 2 space orientation, it is Polaroid can be to multiple quantum dots
2 are positioned, and locating speed is greatly improved.The present invention combines the positioning of quantum dot 2 and temperature measurement, realizes nanometer
Grade three dimensional temperature field measurement.
Detailed description of the invention
Fig. 1 is structural schematic diagram of the invention.
Specific embodiment
The present invention is described in further detail below in conjunction with the accompanying drawings.
Referring to Fig.1, a kind of nanoscale three-dimensional quantum thermal imaging system, including laser 1, in the laser optical path that laser 1 issues
On be disposed with objective table 3, object lens 4 and half-reflecting half mirror 5, the sample containing several quantum dots 2 is placed on objective table 3,
Laser 1 issues laser irradiation on sample, and sample and quantum dot 2 are imaged through the amplification of object lens 4, then act on through half-reflecting half mirror 5
It is divided into two-way optical path, is all the way reflected light path, is all the way transmitted light path.
Be disposed on the reflected light path of half-reflecting half mirror 5 first filter plate 6, the first lens 7, phase-modulator 8,
The reflected light of second lens 9, CCD10, half-reflecting half mirror 5 filters out the laser wave issued with laser 1 through the first filter plate 6
Long inconsistent light reflects obtained image position in the front focal plane of the first lens 7 through half-reflecting half mirror 5, and phase-modulator 8 is located at the
The back focal plane of one lens 7, phase-modulator 8 also are located at the front focal plane of the second lens 9, phase-modulator 8 and computer 14 simultaneously
Connection, 14 real-time control of computer and the modulation function for changing phase-modulator 8, so that the positioning accuracy of quantum dot 2 is controlled,
CCD10 is located at the back focal plane of the second lens 9, and CCD10 is connect with computer 14, and real-time Transmission acquires data.
It is provided with reflecting mirror 11 on the transmitted light path of half-reflecting half mirror 5, is provided on the reflected light path of reflecting mirror 11
Second filter plate 12 and optical spectrum instrumentation 13, the second filter plate 12 filter out light identical with the optical maser wavelength of the sending of laser 1,
The effect of reflecting mirror 11 is change optical path, and light is made to be able to enter optical spectrum instrumentation 13, and optical spectrum instrumentation 13 is connect with computer 14,
Computer 14 obtains spectroscopic data in real time, and according to the Photothermal characterisation of quantum dot 2, calculates 2 environment temperature of quantum dot.
The phase-modulator 8 is stationary phase plate, or is transmission liquid crystal phase-modulator, or is reflection type liquid crystal
Phase-modulator.
The operation principle of the present invention is that:
Quantum dot 2 in sample images in the front focal plane of the first lens 7, and the first lens 7 carry out Fourier transformation simultaneously to it
Back focal plane is imaged in, the modulation function of phase-modulator 8 is controlled by computer 14, the light modulated is imaged through the second lens 9
In on CCD10, by the modulation function of phase-modulator 8, quantum dot 2 can be imaged as to two specks, speck line center
Reflect the lateral position of quantum dot 2, the angle reflection quantum dot 2 of line along the position in the laser beam axis direction that laser 1 issues,
The fluorescence of quantum dot 2 enters optical spectrum instrumentation 13 after reflecting mirror 11, the second filter plate 12, and optical spectrum instrumentation 13 measures each amount
The spectrum of son point 2, and computer 14 is returned to, computer 14 calculates each quantum dot 2 according to the Photothermal characterisation of quantum dot 2
Environment temperature, the spatial position of incorporating quantum point 2, obtain sample three dimensional temperature distribution.
Claims (2)
1. a kind of nanoscale three-dimensional quantum thermal imaging system, including laser (1), it is characterised in that: in the laser that laser (1) issues
Objective table (3), object lens (4) and half-reflecting half mirror (5) are disposed in optical path, objective table (3) if on be placed with containing dry measure
The sample of sub- point (2), laser (1) issue laser irradiation on sample, and sample and quantum dot (2) are imaged through object lens (4) amplification,
It is divided into two-way optical path through half-reflecting half mirror (5) effect again, is all the way reflected light path, is all the way transmitted light path,
The first filter plate (6), the first lens (7), phase-modulator are disposed on the reflected light path of half-reflecting half mirror (5)
(8), the reflected light of the second lens (9), CCD (10), half-reflecting half mirror (5) filters out and laser (1) through the first filter plate (6)
The inconsistent light of the optical maser wavelength of sending, through half-reflecting half mirror (5) obtained image position of reflection in the front focal plane of the first lens (7),
Phase-modulator (8) is located at the back focal plane of the first lens (7), and phase-modulator (8) also is located at the preceding coke of the second lens (9) simultaneously
Face, phase-modulator (8) are connect with computer (14), and CCD (10) is located at the back focal plane of the second lens (9), CCD (10) and calculating
Machine (14) connection,
It is provided with reflecting mirror (11) on the transmitted light path of half-reflecting half mirror (5), is arranged on the reflected light path of reflecting mirror (11)
There are the second filter plate (12) and optical spectrum instrumentation (13), the second filter plate (12) filters out the laser wave issued with laser (1)
Long identical light, optical spectrum instrumentation (13) are connect with computer (14).
2. a kind of nanoscale three-dimensional quantum thermal imaging system according to claim 1, it is characterised in that: the phase-modulator
(8) it is stationary phase plate, or is transmission liquid crystal phase-modulator, or is reflection type liquid crystal phase-modulator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610157837.6A CN105651394B (en) | 2016-03-18 | 2016-03-18 | A kind of nanoscale three-dimensional quantum thermal imaging system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610157837.6A CN105651394B (en) | 2016-03-18 | 2016-03-18 | A kind of nanoscale three-dimensional quantum thermal imaging system |
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| Publication Number | Publication Date |
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| CN105651394A CN105651394A (en) | 2016-06-08 |
| CN105651394B true CN105651394B (en) | 2019-01-29 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107703027B (en) * | 2017-09-26 | 2020-08-14 | 西安交通大学 | Method for measuring cytoplasm viscosity based on quantum dot three-dimensional tracing |
| CN110081996B (en) * | 2019-04-28 | 2020-10-27 | 西安交通大学 | Quantum dot space tracing and temperature measuring system and method |
| CN110274708B (en) * | 2019-07-12 | 2020-04-10 | 西安交通大学 | Tumor cell nanoscale quantum three-dimensional thermal imaging system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2005088783A1 (en) * | 2002-01-28 | 2005-09-22 | Board Of Trustees Operating Michigan State University | Laser system using ultra-short laser pulses |
| CN101601607A (en) * | 2009-05-22 | 2009-12-16 | 东南大学 | A kind of tumor cell is carried out magnetic induction heating, imaging and thermometric method simultaneously |
| CN101653355B (en) * | 2009-09-11 | 2012-01-11 | 华中科技大学 | Living small animal imaging system and imaging method |
| US9314304B2 (en) * | 2010-12-08 | 2016-04-19 | Lumicell, Inc. | Methods and system for image guided cell ablation with microscopic resolution |
| CN103411703B (en) * | 2013-07-18 | 2015-06-03 | 西安交通大学 | Non-contact-type temperature measurement method based on cadmium telluride quantum dot photoluminescence |
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Granted publication date: 20190129 |