CN105640665A - Stress loading device and system for constructing stress fracture animal model and method - Google Patents
Stress loading device and system for constructing stress fracture animal model and method Download PDFInfo
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Abstract
The invention discloses a stress loading device and system for constructing a stress fracture animal model and a method, and belongs to the technical field of animal experiment models. The device comprises a tissue fixing unit for fixing experimental animal skeletons, a laser displacement sensor is arranged at one side of the tissue fixing unit, and a stress sensor is arranged at the other side of the tissue fixing unit. A linear actuator is arranged at the other end of the stress sensor, and the tissue fixing unit, the stress sensor and the linear actuator are coaxially arranged. A linear guide rail is further arranged below the tissue fixing unit. The invention further discloses a controllable stress fracture animal model construction system based on the stress loading device. The system comprises the stress loading device, a real-time data collecting and processing module and a PC side LabVIEW control program. The device is precise and controllable in loading strength and time, and the success rate of establishment of the model can be effectively increased.
Description
Technical field
The invention belongs to animal experimental model technical field, be specifically related to a kind of stress loading device building stress fracture animal model and loading system and method.
Background technology
Stress fracture (SF) is a kind of fatigue fracture often betiding normal sclerotin, cannot absorb in time after excessively using fatigue due to muscle and shake produced by impact several times, and make stress conduction to skeleton cause the detectable micro-damage of ability under a kind of microscope. If this micro-damage constantly accumulates, exceed the ability that body bone self is repaired, stress fracture will be produced. In the common training being common in military training and athlete and dancer of stress fracture, the not still research emphasis of military medicine and difficult point, is also one of research topic of paying close attention to of sports medical science simultaneously.
Breihaupt first time in 1855 has formally delivered soldier after studying long-haul march and the article of foot stress fracture has occurred, so stress fracture is also known as " march fracture ". Many scientists also report the stress fracture of different parts in military training and training in succession subsequently, including tibiofibula common in soldier and running athlete and femur stress fracture, throw the ulna stress fracture in type games person, and be common in the rib stress fracture etc. rowed the boat in rower. During China's new recruit's grounding, lower limb stress fracture has become main Injuries In Military Training, has a strong impact on that soldier is healthy and combat effectiveness of the troops. The military training that certain portion 1861 soldier carries out is caused the retrospective annual epidemics disease he result of investigation of lower limb stress fracture show according within 2008, explaining the height of trees and the dish space that shakes, it is 6.2% that military training causes the annual sickness rate of lower limb stress fracture, sickness rate and the length of military service are obvious negative correlation (P < 0.01), based on tibiofibula and fracture of the metatarsal bone, account for the 49.6% and 35.7% of fracture sum respectively, long-time rapid march and 5 km cross-country races are subjects of mainly causing injury, over loading or in the short time super-strength training be main causative factors of injury. So, cause the incidence rate of lower limb stress fracture in order to reduce military training, improve the physical condition of officers and men, improve combat effectiveness of the troops, it is instant for finding a kind of method that can successfully manage stress fracture generation.
But, mostly the treatment now tackling stress fracture both at home and abroad is the measure that fracture is taked after occurring, mainly there are plaster fixing, operative treatment, lie up for a long time, formulate the universal of reasonable training plan and stress fracture relevant knowledge and explain and publicise, and tackle stress fracture problem, the meaning of prevention is significantly larger than treatment, explore effective stress fracture preventive measure and there is highly important military benefit and social benefit, but there is not yet the relevant report of the medical intervention measure that can effectively reduce stress fracture incidence rate at present both at home and abroad.It is monitored the method with early warning in order to help research pulsed electromagnetic fields associating diphosphate to carry out prevention better with bio-electrical impedance technology counter stress fracture, sets up stress fracture animal model generating means sufficiently reliable, science and be necessary.
The modeling method of domestic common stress fracture has: swimming instruction method, and race method and treadmill training method are jumped in electricity irritation. Latter two is all be placed in runway or cage by animal, runs with means stimulating animals such as acousto-optic-electrics or jumps, but all equally exist modeling period length, problem that positive rate is low. The model that Wang Ping Nan and Huang Changlin sets up with animal treadmill, its stress fracture positive rate only has 22.2%. Although Song Tian first-class forces it to move by experimental rabbit applies high voltage stimulation, the frequency of stimulation can be regulated and intensity that the time moves with Control release rabbit, increase and be modeled as power, but it is also only 37.5%, still fails to be fully solved that fracture positive rate is low, the problem of modeling period length, and, when frequency of training increases, easily there is toleration in animal, affects training precision and effect, and the mortality rate of laboratory animal is higher. Burr etc. adopt pulling method to establish rabbit stress fracure of tibia animal model, and embodiment is to fix rabbit tibia to its applying axial tensile stress with clamping plate, and strain gauge detects its strain in real time simultaneously. The method energy accurately control load size, monitors strain variation in real time. But, tensile loads pattern is difficult to generation and the development of stress fracture under simulation physiological status. It addition, current all of stress fracture modeling method is required for laboratory animal is anaesthetized, just a situation arises really with stress fracture more disconnects mutually for this.
Clinical basic needs be easy to get, standardized animal model, but modeling method external at present makes process relatively complicated time-consuming due to needs anesthesia, domestic modeling method is all then indirectly skeleton is applied stress by the motion of stimulating animal muscle over loading, owing to each stimulation starting point is different, the quality being likely to result in model is uneven, and the positive rate of model and standardization all cannot meet requirement. Therefore, stress fracture animal model build it is crucial that propose a kind of quantitative, controlled, without anesthesia and become the high new Modeling of mould rate.
Summary of the invention
For the defect overcoming above-mentioned prior art to exist, it is an object of the invention to provide a kind of stress loading device building stress fracture animal model and loading method, this device density of load, time controllable precise, it is possible to be effectively improved into mould rate.
The present invention is achieved through the following technical solutions:
A kind of stress loading device building stress fracture animal model, including the tissue fixed cell for fixing laboratory animal skeleton, it is provided with laser displacement sensor in the side of tissue fixed cell, opposite side is provided with strain gauge, the other end of strain gauge is provided with linear actuator, tissue fixed cell, strain gauge and linear actuator are coaxially disposed, and are additionally provided with linear guides below tissue fixed cell.
Tissue fixed cell includes clamp head and dynamic chuck, and clamp head is positioned at laser displacement sensor side, and dynamic chuck is positioned at strain gauge side, and linear guides is arranged on the lower section of dynamic chuck.
When setting up animal ulna stress fracture animal model, tissue fixed cell includes the ulna clamp head for fixing laboratory animal elbow joint, and is used for fixing the dynamic chuck of the carpal ulna of laboratory animal.
When setting up animal stress fracure of tibia animal model, tissue fixed cell includes the tibia clamp head for fixing laboratory animal proximal ends of tibia joint and the dynamic chuck of tibia for fixing ankle.
Above tibia clamp head and above the dynamic chuck of tibia, one piece of tabletting for preventing tibia from slipping respectively is set.
Tibia clamp head is arranged above the long tabletting of tibia, and the dynamic chuck of tibia is arranged above the short tabletting of tibia.
The invention also discloses a kind of controlled stress fracture animal model constructing system, including above-mentioned stress loading device, real-time data acquisition and processing module and the PC end loading LabVIEW control program;
Described real-time data acquisition and processing module are made up of signal modulated amplifier, the motion controller of linear actuator and data collecting card, the analog voltage signal that strain gauge and laser displacement sensor export is modulated amplifying and inputting the acquisition channel of data collecting card by signal modulated amplifier, data collecting card converts analog signals into digital signal, control program by the LabVIEW of PC end signal to carry out Real-time Collection, processes and storage, real-time output pressure and displacement; The outfan of the motion controller of linear actuator is connected with PC end either directly through USB interface, LabVIEW program the position of linear actuator be acquired and show.
The invention also discloses the method building stress fracture animal model based on above-mentioned controlled stress fracture animal model constructing system, comprise the following steps:
1) by linear guides regulate tissue fixed cell shifting axle from, make the skeleton to be measured of laboratory animal be placed in tissue fixed cell;
2) controlled displacement and the speed of the motion of program setting linear actuator by LabVIEW, the skeleton to be measured of laboratory animal is applied certain stress loading;
Wherein, stress loading adopts loop cycle load mode, and the basic waveform loaded is that slope loads; In stress loading process, the surge pressure of loading keeps consistent;
3) stress suffered by the skeleton to be measured of laboratory animal and deformation are detected by strain gauge and laser displacement sensor in real time, are transferred to PC end by data collecting card and are carried out display in real time and the storage of data by LabVIEW program.
When setting up animal ulna stress fracture animal model, arranging the prestrain of 1.5N, 0.8 second ramp up and the ramp down of 0.8 second, the waiting time between two loading cycles is 0.1 second; Surge pressure arranges and ranges for 30��40N, and loading cycle is 4000��5000 times.
When setting up animal stress fracure of tibia animal model, arranging the prestrain of 5.0N, 0.8 second ramp up and the ramp down of 0.8 second, the waiting time between two loading cycles is 0.1 second; Surge pressure arranges and ranges for 50��70N, and loading cycle is 5000��7000 times
Compared with prior art, the present invention has following useful technique effect:
The stress loading device of structure stress fracture animal model disclosed by the invention, including laser displacement sensor, tissue fixed cell and linear actuator, it is connected by strain gauge between tissue fixed cell and linear actuator, with the compression stress that experiments of measuring animal osseous part bears, this device density of load, time controllable precise, it is possible to be effectively improved into mould rate.
Further, this device can set up rat tibia and two kinds of stress fracture animal models of ulna, it is possible to achieve compression, stretch, compression is collaborative stretches three kinds of loading modes, it is adaptable to Animal Anesthesia and without anesthesia two states.
The invention also discloses the controlled structure stress fracture animal model constructing system based on above-mentioned stress loading device, control program including stress loading device, real-time data acquisition and processing module and PC end LabVIEW. The displacement moved by the manual control part setting linear actuator of LabVIEW control program and speed are in order to apply certain prestrain, and laboratory animal skeleton institute's stress and deformation are detected by strain gauge and laser displacement sensor in real time simultaneously. This stress loading system defines closed loop feedback system, it is possible to ensure to apply intensity is controlled, the time is controlled and pattern is consistent cyclic load, and the pressure that bears of test experience animal skeleton that can be accurate, real-time and the position of bone move. The stress loading device of the present invention close to the situation of true stress fracture, lays the foundation for ensureing into mould rate to greatest extent, for studying the pathogenesis of stress fracture, exploring valuable prevention and method for early warning provides important model supports.
The method of structure stress fracture animal model disclosed by the invention, by linear guides regulate tissue fixed cell shifting axle from, the osseous part to be measured making laboratory animal is placed in tissue fixed cell, and the displacement moved by the manual control part setting linear actuator of LabVIEW control program and speed are in order to apply certain prestrain. Stress loading adopts loop cycle load mode, and the basic waveform loaded is that slope loads, and including the parameter such as speed and time setting the periodicity loaded, the speed ramped up and time and decline, tibia or ulna is circulated reciprocal loading. System is operated in Stress Control pattern in stress loading process, and namely in each pressing period, the baseline of minimum on-load pressure keeps consistent, and maximum load pressure also keeps consistent substantially.
Accompanying drawing explanation
Fig. 1 is the stress loading apparatus structure schematic diagram that the present invention builds the stress fracture animal model of rat ulna;
Fig. 2 is the stress loading apparatus structure schematic diagram that the present invention builds the stress fracture animal model of rat tibia;
Fig. 3 is the tissue fixed chuck structural representation that the present invention builds rat ulna;
Fig. 4 is the tissue fixed chuck structural representation of the rat tibia that the present invention builds;
Fig. 5 is the stress loading device data acquisition control system structured flowchart that the present invention builds stress fracture animal model;
Fig. 6 adopts apparatus of the present invention that rat tibia applies the stress loading curve chart of detection when axial cyclic loads;
Fig. 7 is the SPECT/CT scanning result figure using apparatus of the present invention to set up rat tibia stress fracture animal model;
Fig. 8 is the microCT scanning result figure using apparatus of the present invention to set up rat ulna stress fracture animal model.
Wherein, 1 is laser displacement sensor; 2 is ulna clamp head; 3 is the dynamic chuck of ulna; 4 is strain gauge; 5 is linear actuator; 6 is linear guides; 7 is tibia clamp head; 8 is the dynamic chuck of tibia; 9 is the long tabletting of tibia; 10 is the short tabletting of tibia.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail, and the explanation of the invention is not limited.
Embodiment 1
Referring to Fig. 1, the stress loading apparatus structure of the stress fracture animal model building rat ulna disclosed by the invention, including the tissue fixed cell for fixing laboratory animal skeleton, it is provided with laser displacement sensor 1 in the side of tissue fixed cell, opposite side is provided with strain gauge 4, the other end of strain gauge 4 is provided with linear actuator 5, and tissue fixed cell, strain gauge 4 and linear actuator 5 are coaxially disposed, and is additionally provided with linear guides 6 below tissue fixed cell.Wherein, tissue fixed cell includes the ulna clamp 2 for fixing laboratory animal elbow joint, and is used for fixing the dynamic chuck 3 of the carpal ulna of laboratory animal and forms, referring to Fig. 3.
When setting up the animal model of rat ulna stress fracture, being fixed on by ulna in tissue fixed cell, adopt ulna clamp 2 to block the elbow joint of laboratory animal, the dynamic chuck 3 of ulna effectively fixes carpal joint, it is prevented that laterally deviate from. Tissue fixed cell is outer to be fixed by linear guides, to prevent the generation of lateral displacement. It is connected by strain gauge 4 between tissue fixed cell and linear actuator 5, to measure the compression stress that tibia bears. LabVIEW programme-control linear actuator 4 moves and ulna is applied axial stress, and ulna institute's stress and deformation are detected by strain gauge 4 and laser displacement sensor 1 in real time simultaneously.
Referring to Fig. 5, real-time data acquisition and processing module are made up of signal modulated amplifier, the motion controller of linear actuator and data collecting card. The analog voltage signal that strain gauge and displacement transducer export is modulated amplifying and inputting the acquisition channel of data collecting card by signal modulated amplifier, data collecting card converts analog signals into digital signal, control program by the LabVIEW of PC end signal to carry out Real-time Collection, processes and storage, with conversion formula, magnitude of voltage is converted to force value and the shift value of correspondence respectively, real-time output pressure and displacement, and show the relation curve of pressure versus time, displacement versus time, pressure-displacement in real time. The outfan of motion controller is connected with PC end either directly through USB interface, LabVIEW program the position of linear actuator be acquired and show. This stress loading system can control program by LabVIEW and send commands to motion controller to control the motion of linear actuator, it is possible to produce uniform motion pressurization, recurrent pulse pressurization, slope loads and compression and tension loads four kinds of patterns.
In order to evaluate the certainty of measurement of this stress loading device, this stress loading system is verified by we by using the long cylindrical sample for polyethylene (UHMWPE) material that 1cm, diameter are 1cm super high molecular weight. its method includes, use the stress loading device in the present invention that this UHMWPE small cylinder sample is carried out axial compression experiment, this sample is fixed in the middle of tissue fixator, prestrain is sized to 0.5N, what guarantee this sample axially retains level completely, control program by PC end LabVIEW and send order to linear actuator, it is made to apply the axial compression load of 10 ��m/sec, until the load that sample bears reaches 15N, stop the action of linear actuator at once, real-time record displacement and stress relation over time, the Young's modulus (Young'sModulus) of this sample is calculated by below equation:
In formula, E is elastic modelling quantity, and F is compression stress, and S is the cross-sectional area of UHMWPE small cylinder sample, and �� L is the compressive deformation of UHMWPE small cylinder sample, and L is the length of small cylinder sample.
Adopt universal testing machine (BoseElectroForce3220, Bose, USA) the same Young's modulus obtaining this UHMWPE small cylinder sample, the load mode adopted and loading parameters are identical with what the stress loading system in the present invention used, the same Young's modulus calculating this UHMWPE small cylinder standard material of acquisition. The relatively measurement result of the two, thus the certainty of measurement of the stress loading device assessed in the present invention. It is 252MPa that universal material test machine obtains the Young's modulus standard value of UHWPE, should the Young's modulus value that records of force loading system be 260MPa, and error is 3.17%, it was shown that the stress loading device designed by the present invention has good measurement precision.
Embodiment 2
Referring to Fig. 2, the stress loading apparatus structure of the stress fracture animal model of structure rat tibia disclosed by the invention, including the tissue fixed cell for fixing laboratory animal skeleton, it is provided with laser displacement sensor 1 in the side of tissue fixed cell, opposite side is provided with strain gauge 4, the other end of strain gauge 4 is provided with linear actuator 5, and tissue fixed cell, strain gauge 4 and linear actuator 5 are coaxially disposed, and is additionally provided with linear guides 6 below tissue fixed cell. Wherein, tissue fixed cell includes the tibia clamp 7 for fixing laboratory animal proximal ends of tibia joint, and the dynamic chuck 8 of tibia for fixing ankle, above tibia clamp 7 and above the dynamic chuck 8 of tibia, one piece of tabletting for preventing tibia from slipping respectively is set, tibia clamp 7 is arranged above the long tabletting 9 of tibia, and the dynamic chuck 8 of tibia is arranged above the short tabletting 10 of tibia. Referring to Fig. 4.
Use should set up rat tibia stress fracture animal model by force loading device, specific embodiment is as follows: 3 monthly age 480g female sd inbred rats are purchased from The Fourth Military Medical University's Experimental Animal Center, rat is anaesthetized by lumbar injection 3% pentobarbital sodium (30mg/kg), left tibias is fixed between the clamp head in tissue stuck-module and dynamic chuck, stress loading adopts cycle dynamic load mode, the basic waveform loaded is that slope loads, prestrain including 5.0N, 0.8 second ramp up and the ramp down of 0.8 second, the Loaded contact analysis that slope spike is 50N is produced respectively by the speed of controlled loading, waiting time between two loading cycles is 0.1 second, loaded for 3000 cycles altogether. as shown in Figure 6, as can be seen from the figure Loaded contact analysis is correct, stable, and surge pressure and baseline are all more steady for the one section of loading curve collected in cyclic stress loading procedure.
Should setting up the effect of rat tibia stress fracture animal model by force loading system to verify, we contact Xijing Nuclear Medicine Department of hospital and the rat after body has loaded are carried out SPECT/CT scanning. SPECT scanning is the one of bone scanning, injection of radioactive substances in advance is needed before scanning, after fully absorbing on skeleton (generally needing 4��6 hours), whole body skeleton Radio-nuclide distribution situation is detected with SPECT, if the abnormal increase of absorption that somewhere skeleton is to radionuclide, namely the abnormal dense poly-phenomenon of radionuclide occurs, then shows that this place's skeleton creates damage. Owing to SPECT scanning figure cannot clearly show the anatomical structure of body, so generally it being combined with CT figure, it is called that SPECT/CT scans.
After body loading experiment completes, inject 5mCi99mTc-MDP developer by rat tail vein, after 5 hours, carry out SPECT/CT scanning. SPECT/CT scanning result is as shown in Figure 7, show that the radionuclide absorbtivity of load side tibia is significantly higher than offside tibia, and CT three-dimensional reconstruction figure shows that complete fracture does not occur load side tibia, it was demonstrated that should can be successfully established rat tibia stress fracture animal model by force loading system.
In addition, we adopt other loading modes to carry out rat tibia testing at body axial cyclic stress loading, result proves that the present invention also can realize rat tibia applies axial cyclic tensile loads tensile loads collaborative with compression, and is applicable to laboratory animal anesthesia and nothing anesthesia two states.
Embodiment 3
Stress loading device disclosed by the invention is used to set up rat ulna stress fracture animal model, specific embodiment is as follows: 5 monthly age 550g male SD rats are purchased from The Fourth Military Medical University's Experimental Animal Center, rat is anaesthetized by lumbar injection 3% pentobarbital sodium (30mg/kg), right side ulna is fixed between the clamp head in tissue stuck-module and dynamic chuck, stress loading adopts cycle dynamic load mode, the basic waveform loaded is that slope loads, prestrain including 1.5N, 0.8 second ramp up and the ramp down of 0.8 second, the Loaded contact analysis that slope spike is 30N is produced respectively by the speed of controlled loading, waiting time between two loading cycles is 0.1 second, loaded for 5000 cycles altogether.
After body loading experiment completes, microCT is adopted to scan contrast experiment's end and the micro-damage of comparison end ulna, as shown in Figure 8, right side loading end ulna mid-diaphysis is horizontal micro-crack and bigger longitudinal crack as seen, left side is then without this micro-damage, prove that present invention success produces micro-damage at rat 1/3, be successfully established rat ulna stress fracture animal model.
In sum, controlled structure stress fracture animal model constructing system disclosed by the invention, control program including stress loading device, real-time data acquisition and processing module and PC end LabVIEW. Described stress loading device is mainly made up of linear actuator, strain gauge, linear guides, tissue fixed cell (including dynamic chuck and clamp head) and displacement transducer.
When setting up the animal model of rat tibia stress fracture, tibia is fixed in tissue fixed cell, clamp head blocks proximal ends of tibia joint, dynamic chuck effectively fixes ankle, preventing from laterally deviating from, clamp head and dynamic chuck upper end are respectively arranged with one piece of tabletting in order to prevent tibia from pressing from both sides prolapse upwards during the course.
When setting up the animal model of rat ulna stress fracture, being fixed on by ulna in tissue fixed cell, clamp head blocks the elbow joint of laboratory animal, and dynamic chuck effectively fixes carpal joint, it is prevented that laterally deviate from. Tissue fixed cell is outer to be fixed by linear guides, to prevent the generation of lateral displacement. It is connected by strain gauge between tissue fixed cell and linear actuator, to measure the compression stress that tibia bears. Tibia is applied axial stress by the motion of LabVIEW programme-control linear actuator, and simultaneously tibia or ulna institute's stress and deformation are detected by power and displacement transducer in real time.
The invention also discloses and carry out, with aforementioned controlled stress fracture animal model constructing system, the new method that stress loading effect drag builds, regulate shifting axle to adjust the distance between clamp head and dynamic chuck, make tibia or ulna can be placed in tune with in fixture, being fixed in tissue stuck-module by rat tibia or ulna, the displacement moved by the manual control part setting linear actuator of LabVIEW control program and speed are in order to apply certain prestrain. Stress loading adopts loop cycle load mode, and the basic waveform loaded is that slope loads, and including the parameter such as speed and time setting the periodicity loaded, the speed ramped up and time and decline, tibia or ulna is circulated reciprocal loading. System is operated in Stress Control pattern in stress loading process, and namely in each pressing period, the baseline of minimum on-load pressure keeps consistent, and maximum load pressure also keeps consistent substantially.
This set of novel stress fracture animal model constructing system defines closed loop feedback, it is ensured that apply intensity is controlled, the time is controlled, pattern is consistent cyclic load, and the pressure that bears of detection skeleton that can be accurate, real-time and the position of bone move. Additionally, this device can realize compression, stretches, compression is collaborative stretches three kinds of loading modes, it is adaptable to Animal Anesthesia and without anesthesia two states, it is possible to set up rat tibia and two kinds of stress fracture animal models of ulna. The situation that this Novel Modeling device and method reliably and is to greatest extent fractured close to true stress, lay the foundation for ensureing into mould rate, for studying the pathogenesis of stress fracture, exploring valuable prevention and method for early warning provides important model supports.
Claims (10)
1. the stress loading device building stress fracture animal model, it is characterized in that, including the tissue fixed cell for fixing laboratory animal skeleton, it is provided with laser displacement sensor (1) in the side of tissue fixed cell, opposite side is provided with strain gauge (4), the other end of strain gauge (4) is provided with linear actuator (5), tissue fixed cell, strain gauge (4) and linear actuator (5) are coaxially disposed, and are additionally provided with linear guides (6) below tissue fixed cell.
2. the stress loading device of structure stress fracture animal model according to claim 1, it is characterized in that, tissue fixed cell includes clamp head and dynamic chuck, clamp head is positioned at laser displacement sensor (1) side, dynamic chuck is positioned at strain gauge (4) side, and linear guides (6) is arranged on the lower section of dynamic chuck.
3. the stress loading device of structure stress fracture animal model according to claim 2, it is characterized in that, when setting up animal ulna stress fracture animal model, tissue fixed cell includes ulna clamp head (2) for fixing laboratory animal elbow joint, and is used for fixing the dynamic chuck (3) of the carpal ulna of laboratory animal.
4. the stress loading device of structure stress fracture animal model according to claim 2, it is characterized in that, when setting up animal stress fracure of tibia animal model, tissue fixed cell includes tibia clamp head (7) for fixing laboratory animal proximal ends of tibia joint and the dynamic chuck (8) of tibia for fixing ankle.
5. the stress loading device of structure stress fracture animal model according to claim 4, it is characterised in that dynamic chuck (8) top respectively arranges one piece of tabletting for preventing tibia from slipping in tibia clamp head (7) top and tibia.
6. the stress loading device of structure stress fracture animal model according to claim 5, it is characterized in that, tibia clamp head (7) is arranged above the long tabletting of tibia (9), and tibia moves chuck (8) and is arranged above the short tabletting of tibia (10).
7. a controlled stress fracture animal model constructing system, it is characterised in that include in claim 1��6 the stress loading device described in any one, real-time data acquisition and processing module and load LabVIEW and control the PC end of program;
Described real-time data acquisition and processing module are made up of signal modulated amplifier, the motion controller of linear actuator (5) and data collecting card, the analog voltage signal that strain gauge (4) and laser displacement sensor (1) export is modulated amplifying and inputting the acquisition channel of data collecting card by signal modulated amplifier, data collecting card converts analog signals into digital signal, control program by the LabVIEW of PC end signal to carry out Real-time Collection, processes and storage, real-time output pressure and displacement; The outfan of the motion controller of linear actuator (5) is connected with PC end either directly through USB interface, by LabVIEW program, the position of linear actuator (5) is acquired and is shown.
8. the method building stress fracture animal model based on the controlled stress fracture animal model constructing system described in claim 7, it is characterised in that comprise the following steps:
1) by linear guides (6) regulate tissue fixed cell shifting axle from, make the skeleton to be measured of laboratory animal be placed in tissue fixed cell;
2) control, by LabVIEW, displacement and the speed that program setting linear actuator (5) moves, the skeleton to be measured of laboratory animal is applied certain stress loading;
Wherein, stress loading adopts loop cycle load mode, and the basic waveform loaded is that slope loads;In stress loading process, the surge pressure of loading keeps consistent;
3) stress suffered by the skeleton to be measured of laboratory animal and deformation are detected by strain gauge (4) and laser displacement sensor (1) in real time, are transferred to PC end by data collecting card and are carried out display in real time and the storage of data by LabVIEW program.
9. the method for structure stress fracture animal model according to claim 8, it is characterized in that, when setting up animal ulna stress fracture animal model, arranging the prestrain of 1.5N, 0.8 second ramp up and the ramp down of 0.8 second, the waiting time between two loading cycles is 0.1 second; Surge pressure arranges and ranges for 30��40N, and loading cycle is 4000��5000 times.
10. the method for structure stress fracture animal model according to claim 8, it is characterized in that, when setting up animal stress fracure of tibia animal model, arranging the prestrain of 5.0N, 0.8 second ramp up and the ramp down of 0.8 second, the waiting time between two loading cycles is 0.1 second; Surge pressure arranges and ranges for 50��70N, and loading cycle is 5000��7000 times.
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