CN105617715A - Method for removing arsenic from termite extracts by virtue of adsorption of novel resin - Google Patents

Method for removing arsenic from termite extracts by virtue of adsorption of novel resin Download PDF

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CN105617715A
CN105617715A CN 201610027703 CN201610027703A CN105617715A CN 105617715 A CN105617715 A CN 105617715A CN 201610027703 CN201610027703 CN 201610027703 CN 201610027703 A CN201610027703 A CN 201610027703A CN 105617715 A CN105617715 A CN 105617715A
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termites
resin
extract
termite
mass
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CN 201610027703
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Chinese (zh)
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高国文
李成林
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江西中天医药生物有限公司
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56Materials from animals other than mammals
    • A61K35/63Arthropods
    • A61K35/64Insects, e.g. bees, wasps or fleas
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361Ion-exchange
    • B01D15/362Cation-exchange
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804Affinity chromatography
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804Affinity chromatography
    • B01D15/3828Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D2015/3838Ligand exchange chromatography, e.g. complexation chromatography, chelation chromatography, metal interaction chromatography
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2221/00Applications of separation devices
    • B01D2221/10Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues

Abstract

The invention discloses a method for removing arsenic from termite extracts by virtue of adsorption of novel resin. According to the method, termite extraction liquid is extracted from termite by virtue of compound enzyme, arsenic is removed from the termite extracts by virtue of the specific resin and a specific resin treatment method, the contents of other effective components in the extraction liquid are not influenced when arsenic ions are removed, the removal rate of arsenic reaches 70% and above, and after arsenic ions are removed, the termite extraction liquid is concentrated and dried and then can be utilized for producing health products. The method is simple, the effect is remarkable, the effective components of products are not influenced, and secondary pollution is avoided; the method is a good method applicable to large-scale production.

Description

一种用新型树脂吸附去除白蚁提取物中砷的方法 Adsorption with one kind of novel termite extract arsenic removal

技术领域 FIELD

[0001] 本发明涉及一种去除白蚁提取物中砷的方法,尤其涉及一种用新型树脂吸附去除白蚁提取物中砷的方法。 [0001] The present invention relates to a method termites extract arsenic removal, in particular, relates to a novel resin termite extract arsenic adsorbed and removed.

背景技术 Background technique

[0002] 白蚁(主要指黄翅大白蚁,黑翅土白蚁,家白蚁。)具有一定的药用价值,和保健功能。 [0002] termites (mainly refers to the yellow-winged termites, black-winged termites, termite.) Has some medicinal value, and health care functions. 近些年用白蚁研究开发的保健品较多,他具有抗疲劳,减少人体化学性肝损伤等方面的功能功效。 In recent years, with more research and development of health products termites, he has anti-fatigue, reduced functionality efficacy of the human body chemical liver injury. 但由于白蚁自身的生活环境和习性,使其自身含砷非常高,白蚁提取物中砷的含量是保健品标准要求的几倍或几十倍,直接影响到白蚁的开发利用。 However, due to termites own living environment and habits, lends itself to a very high arsenic content of arsenic in the extract termites health care standards is several times or several times, directly affect the development and utilization of termites.

发明内容 SUMMARY

[0003] 本发明的目的是提供一种用新型树脂吸附去除白蚁提取物中砷的方法,采用一种新型树脂有效吸附白蚁提取液中的砷离子,砷的去除率达到70%以上,而且在去除砷离子的同时不影响提取液中的其他有效成分的含量。 [0003] The object of the present invention is to provide a new type resin termite extract arsenic adsorbed and removed, using a new efficient adsorption of arsenic ions termites resin extract the arsenic removal efficiency of 70% or more, and in while removing arsenic ions do not affect the content of the other active ingredients in the extract.

[0004] -种用新型树脂吸附去除白蚁提取物中砷的方法,具体步骤为: (1) 白蚁提取液的制备白蚁经清洗、干燥后制得白蚁干品,称重后粉碎过60目筛为白蚁粉,备用, 将粉碎好的白蚁粉加入提取罐,加入8-10倍质量的纯化水,再加入2%白蚁粉质量的脂肪酶和4%白蚁粉质量的蛋白酶,在温度40-45度的条件下酶解6-8小时, 酶解液从提取罐放出经80-100目初滤,再经离心机过滤,收集滤液即为白蚁提取液,备用; (2) 树脂选用和处理选用ZG S 820型树脂,将树脂装入树脂罐,用2BV的水以2BV/h的流速洗涤,再用2BV 质量浓度为4%的氢氧化钠溶液以4-6 BV/h的流速通过树脂,备用; (3) 提取液除砷离子将(1)项中制备的白蚁提取液通过树脂,流速为2-3 BV/h,树脂与白蚁干品的质量比是1:1,温度为25°C-60°C,pH<10, 收集通过树脂的白蚁提取液,经浓缩、干燥后得到白蚁提取物干粉 [0004] - a new resin termite species extract arsenic adsorbed and removed, the specific steps: Preparation of the (1) termites termites extract was washed and dried to obtain termites dried, weighed 60 mesh crushed termite powder, standby, pulverized powder is added termite good extraction tank, purified water was added 8-10 times the mass of powder was added 2% by mass termite lipase powder and 4% by mass of termites protease at a temperature of 40-45 6-8 hours at hydrolysis degree, enzyme release was filtered through 80-100 mesh from First extraction tank, centrifuge and then by filtration, the filtrate is collected termites extract standby; (2) a resin selection and selection process ZG S 820 resin, the resin was charged resin tank, washed with water 2BV flow 2BV / h, and then 2BV mass concentration of 4% sodium hydroxide solution at a flow rate of 4-6 BV / h through the resin, alternate; (3) in addition to extract arsenic ions termites extract prepared in item (1) is passed through the resin flow rate of 2-3 BV / h, the mass ratio of the resin is dried termites 1: 1, a temperature of 25 ° C-60 ° C, pH <10, was collected by termites extract resin, concentrated, and dried to give a dry powder extract termites ,用于保健品生产。 For health care products.

[0005] -种用新型树脂吸附去除白蚁提取物中砷的方法,具体步骤为: (1) 白蚁提取液的制备白蚁经清洗、干燥后制得白蚁干品,称重后粉碎过60目筛为白蚁粉,备用, 将粉碎好的白蚁粉加入提取罐,加入8-10倍质量的纯化水,再加入2%白蚁粉质量的脂肪酶和4%白蚁粉质量的蛋白酶,在温度40-45度的条件下酶解6-8小时, 酶解液从提取罐放出经80-100目初滤,再经离心机过滤,收集滤液即为白蚁提取液,备用; (2) 树脂选用和处理选用ZG S 820型大孔树脂,将树脂装入树脂罐,先用2BV的水以2BV/h的流速洗涤,再用2BV质量浓度为4%的氢氧化钠溶液以4-6 BV/h的流速通过树脂,继续用IBV质量浓度为2%的氢氧化钠溶液以2-4 BV/h的流速通过树脂,最后用0.5V质量浓度为1%的氢氧化钠溶液以1-2 BV/h的流速通过树脂,备用; (3)提取液除砷离子将(1)项中制备的白蚁提取液通过树脂,流 [0005] - a new resin termite species extract arsenic adsorbed and removed, the specific steps: Preparation of the (1) termites termites extract was washed and dried to obtain termites dried, weighed 60 mesh crushed termite powder, standby, pulverized powder is added termite good extraction tank, purified water was added 8-10 times the mass of powder was added 2% by mass termite lipase powder and 4% by mass of termites protease at a temperature of 40-45 6-8 hours at hydrolysis degree, enzyme release was filtered through 80-100 mesh from First extraction tank, centrifuge and then by filtration, the filtrate is collected termites extract standby; (2) a resin selection and selection process ZG S 820 macroporous resin, the resin is charged in a resin tank, first with water and washed with a flow rate of 2BV 2BV / h, and then 2BV mass concentration of 4% sodium hydroxide solution at 4-6 BV / h flow rate a resin, to continue with IBV mass concentration of 2% sodium hydroxide solution at 2-4 BV / h flow rate through the resin, and finally with 0.5V mass concentration of 1% sodium hydroxide solution at 1-2 BV / h of flow rate through the resin, the standby; (3) extract extract arsenic ions other termites of (1) a resin prepared by item, the flow 速为2-3 BV/h,树脂与白蚁干品的质量比是1:1,温度为25°C-60°C,pH<10, 收集通过树脂的白蚁提取液,经浓缩、干燥后得到白蚁提取物干粉,用于保健品生产。 Speed ​​of 2-3 BV / h, the mass ratio of the resin is dried termites 1: 1, a temperature of 25 ° C-60 ° C, pH <10, the resin was collected by termites extract, dried and concentrated, and dried to give termite extract dry powder, for health care products.

[0006] 发明所述的ZG S 820型大孔树脂可以在浙江争光实业股份有限公司购买。 [0006] ZG S 820 macroporous resin according to the invention can be purchased Zhejiang glory for CO., LTD.

[0007] 本发明选用特定的树脂及采用特定的树脂处理方法,来去除白蚁提取物中的砷, 在去除砷离子的同时不影响提取液中的其他有效成分的含量,砷的去除率达到70%以上,白蚁提取液经树脂去除砷离子后,经浓缩、干燥后用于保健品生产。 [0007] The invention selects a particular resin and the particular resin employed processing method to remove arsenic termites extract, does not affect the content of the other active ingredients in the extract solution while removing ions of arsenic, arsenic removal efficiency of 70 % or more, termites extract after removal of arsenic ions with resin, concentrated, dried and used for the production of health care products.

[0008] [0008]

具体实施方式: 实施例1: 一种用新型树脂吸附去除白蚁提取物中砷的方法,具体步骤为: (1) 白蚁提取液的制备白蚁经清洗、干燥后制得白蚁干品,称重后粉碎过60目筛为白蚁粉,备用, 将粉碎好的白蚁粉加入提取罐,加入8倍质量的纯化水,再加入2%白蚁粉质量的脂肪酶和4%白蚁粉质量的蛋白酶,在温度40度的条件下酶解6小时, 酶解液从提取罐放出经80目初滤,再经离心机过滤,收集滤液即为白蚁提取液,备用; (2) 树脂选用和处理选用ZG S 820型树脂,将树脂装入树脂罐,用2BV的水以2BV/h的流速洗涤,再用2BV 质量浓度为4%的氢氧化钠溶液以4BV/h的流速通过树脂,备用; (3) 提取液除砷离子将(1)项中制备的白蚁提取液通过树脂,流速为2BV/h,树脂与白蚁干品的质量比是1: 1,温度为25°(:°(:,?!1<10, 收集通过树脂的白蚁提取液,经浓缩、干燥后得到白 DETAILED DESCRIPTION: Example 1: A resin with a novel termite extract arsenic adsorbed and removed, the specific steps: (1) Preparation of termites termites extract was washed and dried to obtain termites dried, weighed pulverized to 60 mesh powder termites, standby, pulverized powder is added termite good extraction tank, adding 8 times the mass of purified water, then add 2% by mass of powder termite lipase powder and 4% by mass of termites protease, at a temperature 6 hours digestion at 40 °, the early release of enzyme solution was filtered through an 80 mesh from extraction tank, centrifuge and then by filtration, the filtrate is collected termites extract standby; (2) process and the choice of resin selected ZG S 820 resin, the resin was charged resin tank, washed with water 2BV flow 2BV / h, and then 2BV mass concentration of 4% sodium hydroxide solution 4BV / h of flow rate through the resin, the standby; (3) extraction liquid addition arsenic ions termites extract prepared in item (1) is passed through the resin flow rate of 2BV / h, the mass ratio of the resin termites dried is 1: 1, a temperature of 25 ° (: ° (:?!, 1 <10, the resin was collected by termites extract, dried and concentrated, and dried to give a white 蚁提取物干粉,用于保健品生产。 Ant extract powder, used for health care products.

[0009] 实施例2: 一种用新型树脂吸附去除白蚁提取物中砷的方法,具体步骤为: (1) 白蚁提取液的制备白蚁经清洗、干燥后制得白蚁干品,称重后粉碎过60目筛为白蚁粉,备用, 将粉碎好的白蚁粉加入提取罐,加入9倍质量的纯化水,再加入2%白蚁粉质量的脂肪酶和4%白蚁粉质量的蛋白酶,在温度42度的条件下酶解7小时, 酶解液从提取罐放出经100目初滤,再经离心机过滤,收集滤液即为白蚁提取液,备用; (2) 树脂选用和处理选用ZG S 820型树脂,将树脂装入树脂罐,用2BV的水以2BV/h的流速洗涤,再用2BV 质量浓度为4%的氢氧化钠溶液以5BV/h的流速通过树脂,备用; (3) 提取液除砷离子将(1)项中制备的白蚁提取液通过树脂,流速为2.5 BV/h,树脂与白蚁干品的质量比是1:1,温度为40°C,pH<10, 收集通过树脂的白蚁提取液,经浓缩、干燥后得到白蚁提取物干粉,用 [0009] Example 2: once in the new resin termite extract arsenic adsorbed and removed, the specific steps: (1) Preparation of termites termites extract was washed and dried to obtain termites dried, weighed and pulverized 60 mesh powder termites, standby, pulverized powder is added termite good extraction tank, purified water was added to 9 times the mass of powder was added 2% by mass termite lipase powder and 4% by mass of termites protease, at a temperature of 42 hydrolysis of the conditions for 7 hours, enzymatic liquid discharged from the extraction tank through a 100 mesh filter at the beginning, and then filtered through a centrifuge, the filtrate is collected termites extract standby; (2) process and the choice of resin type selection ZG S 820 the resin, the resin was charged resin tank, washed with water 2BV flow 2BV / h, and then 2BV mass concentration of 4% sodium hydroxide solution 5BV / h of flow rate through the resin, the standby; (3) extract in addition to arsenic ions termites extract prepared in item (1) is passed through the resin flow rate of 2.5 BV / h, the mass ratio of the resin termites dried is 1: 1, a temperature of 40 ° C, pH <10, collected by the resin termites extract, concentrated, and dried to give a dry powder extract termites, with 于保健品生产。 In health care products.

[0010] 实施例3: 一种用新型树脂吸附去除白蚁提取物中砷的方法,具体步骤为: (1) 白蚁提取液的制备白蚁经清洗、干燥后制得白蚁干品,称重后粉碎过60目筛为白蚁粉,备用, 将粉碎好的白蚁粉加入提取罐,加入10倍质量的纯化水,再加入2%白蚁粉质量的脂肪酶和4%白蚁粉质量的蛋白酶,在温度45度的条件下酶解8小时, 酶解液从提取罐放出经100目初滤,再经离心机过滤,收集滤液即为白蚁提取液,备用; (2) 树脂选用和处理选用ZG S 820型树脂,将树脂装入树脂罐,用2BV的水以2BV/h的流速洗涤,再用2BV 质量浓度为4%的氢氧化钠溶液以6 BV/h的流速通过树脂,备用; (3) 提取液除砷离子将(1)项中制备的白蚁提取液通过树脂,流速为3 BV/h,树脂与白蚁干品的质量比是1: 1,温度为60°(:,?!1<10, 收集通过树脂的白蚁提取液,经浓缩、干燥后得到白蚁提取物干 [0010] Example 3: Novel resin with one kind of termite extract arsenic adsorbed and removed, the specific steps: (1) Preparation of termites termites extract was washed and dried to obtain termites dried, weighed and pulverized 60 mesh powder termites, standby, pulverized powder is added termite good extraction tank, purified water was added to 10 times the mass of powder was added 2% by mass termite lipase powder and 4% by mass of termites protease, at a temperature of 45 8 hours under the conditions of hydrolysis degree, enzymatic liquid discharged from the extraction tank through a 100 mesh filter at the beginning, and then filtered through a centrifuge, the filtrate is collected termites extract standby; (2) process and the choice of resin type selection ZG S 820 the resin, the resin was charged resin tank, washed with water 2BV flow 2BV / h, and then 2BV mass concentration of 4% sodium hydroxide solution at 6 BV / h flow rate through the resin, the standby; (3) extraction liquid addition arsenic ions termites extract prepared in item (1) is passed through the resin flow rate of 3 BV / h, the mass ratio of the resin termites dried is 1: 1, a temperature of 60 ° (:, 1 <10?! , the extract was collected by termites resin, concentrated, and dried to obtain a dry extract termites ,用于保健品生产。 For health care products.

[0011] 实施例4: 一种用新型树脂吸附去除白蚁提取物中砷的方法,具体步骤为: (1) 白蚁提取液的制备白蚁经清洗、干燥后制得白蚁干品,称重后粉碎过60目筛为白蚁粉,备用, 将粉碎好的白蚁粉加入提取罐,加入8倍质量的纯化水,再加入2%白蚁粉质量的脂肪酶和4%白蚁粉质量的蛋白酶,在温度40度的条件下酶解6小时, 酶解液从提取罐放出经80目初滤,再经离心机过滤,收集滤液即为白蚁提取液,备用; (2) 树脂选用和处理选用ZG S 820型大孔树脂,将树脂装入树脂罐,先用2BV的水以2BV/h的流速洗涤,再用2BV质量浓度为4%的氢氧化钠溶液以4BV/h的流速通过树脂,继续用IBV质量浓度为2%的氢氧化钠溶液以2BV/h的流速通过树脂,最后用0.5V质量浓度为1%的氢氧化钠溶液以IBV/ h的流速通过树脂,备用; (3) 提取液除砷离子将(1)项中制备的白蚁提取液通过树脂,流速 [0011] Example 4: Novel resin with one kind of termite extract arsenic adsorbed and removed, the specific steps: (1) Preparation of termites termites extract was washed and dried to obtain termites dried, weighed and pulverized 60 mesh powder termites, standby, the pulverized powder into an extraction tank termite good, 8 times the mass of purified water was added, and then 2% by mass of powder termite lipase powder and 4% by mass of termites protease, at a temperature of 40 hydrolysis under conditions of six hours, the enzyme was filtered through an 80 mesh discharged early from the extraction tank, centrifuge and then by filtration, the filtrate is collected termites extract standby; (2) process and the choice of resin type selection ZG S 820 macroporous resin, the resin is charged in a resin tank, first with water and washed with a flow rate of 2BV 2BV / h, and then 2BV mass concentration of 4% sodium hydroxide solution 4BV / h of flow rate through the resin mass continues with IBV concentration of 2% sodium hydroxide solution at 2BV / h of flow rate through the resin, and finally with 0.5V mass concentration of 1% sodium hydroxide solution at IBV / h of flow rate through the resin, the standby; (3) extract arsenic removal termites extract ions prepared in (1) item by resin, the flow rate 2BV/h,树脂与白蚁干品的质量比是1: 1,温度为25°C,pH<10, 收集通过树脂的白蚁提取液,经浓缩、干燥后得到白蚁提取物干粉,用于保健品生产。 2BV / h, and the mass ratio of the resin is dried termites 1: 1, a temperature of 25 ° C, pH <10, termites collected in the extract is passed through the resin, concentrated, and dried to give a dry powder extract termites, for health products produce.

[0012] 实施例5: 一种用新型树脂吸附去除白蚁提取物中砷的方法,具体步骤为: (1)白蚁提取液的制备白蚁经清洗、干燥后制得白蚁干品,称重后粉碎过60目筛为白蚁粉,备用, 将粉碎好的白蚁粉加入提取罐,加入9倍质量的纯化水,再加入2%白蚁粉质量的脂肪酶和4%白蚁粉质量的蛋白酶,在温度42度的条件下酶解7小时, 酶解液从提取罐放出经80目初滤,再经离心机过滤,收集滤液即为白蚁提取液,备用; (2) 树脂选用和处理选用ZG S 820型大孔树脂,将树脂装入树脂罐,先用2BV的水以2BV/h的流速洗涤,再用2BV质量浓度为4%的氢氧化钠溶液以5BV/h的流速通过树脂,继续用IBV质量浓度为2%的氢氧化钠溶液以3BV/h的流速通过树脂,最后用0.5V质量浓度为1%的氢氧化钠溶液以1.5BV/h的流速通过树脂,备用; (3) 提取液除砷离子将(1)项中制备的白蚁提取液通过树脂,流速 [0012] Example 5: once in the new resin termite extract arsenic adsorbed and removed, the specific steps: Preparation of the washed termites (1) extract termites, termites after dried to obtain dried, weighed pulverized 60 mesh powder termites, standby, pulverized powder is added termite good extraction tank, purified water was added to 9 times the mass of powder was added 2% by mass termite lipase powder and 4% by mass of termites protease, at a temperature of 42 hydrolysis of the conditions for 7 hours, hydrolysis was filtered through an 80 mesh discharged early from the extraction tank, centrifuge and then by filtration, the filtrate is collected termites extract standby; (2) process and the choice of resin type selection ZG S 820 macroporous resin, the resin is charged in a resin tank, first with water and washed with a flow rate of 2BV 2BV / h, and then 2BV mass concentration of 4% sodium hydroxide solution 5BV / h of flow rate through the resin mass continues with IBV concentration of 2% sodium hydroxide solution at 3BV / h of flow rate through the resin, and finally with 0.5V mass concentration of 1% sodium hydroxide solution 1.5BV / h of flow rate through the resin, the standby; (3) in addition to the extract the arsenic ions termite extract prepared in (1) item by resin, the flow rate 2.5BV/h,树脂与白蚁干品的质量比是1:1,温度为40°C,pH<10, 收集通过树脂的白蚁提取液,经浓缩、干燥后得到白蚁提取物干粉,用于保健品生产。 2.5BV / h, and the mass ratio of the resin is dried termites 1: 1, a temperature of 40 ° C, pH <10, termites collected in the extract is passed through the resin, concentrated, and dried to give a dry powder extract termites, for health goods production.

[0013] 实施例6: 一种用新型树脂吸附去除白蚁提取物中砷的方法,具体步骤为: (1) 白蚁提取液的制备白蚁经清洗、干燥后制得白蚁干品,称重后粉碎过60目筛为白蚁粉,备用, 将粉碎好的白蚁粉加入提取罐,加入10倍质量的纯化水,再加入2%白蚁粉质量的脂肪酶和4%白蚁粉质量的蛋白酶,在温度45度的条件下酶解8小时, 酶解液从提取罐放出经100目初滤,再经离心机过滤,收集滤液即为白蚁提取液,备用; (2) 树脂选用和处理选用ZG S 820型大孔树脂,将树脂装入树脂罐,先用2BV的水以2BV/h的流速洗涤,再用2BV质量浓度为4%的氢氧化钠溶液以6 BV/h的流速通过树脂,继续用IBV质量浓度为2% 的氢氧化钠溶液以4 BV/h的流速通过树脂,最后用0.5V质量浓度为1%的氢氧化钠溶液以2 BV/h的流速通过树脂,备用; (3) 提取液除砷离子将(1)项中制备的白蚁提取液通过树脂,流 [0013] Example 6: new resin with one kind of termite extract arsenic adsorbed and removed, the specific steps: washing the termites prepared by (1) the extract termites, termites after dried to obtain dried, weighed pulverized 60 mesh powder termites, standby, pulverized powder is added termite good extraction tank, purified water was added to 10 times the mass of powder was added 2% by mass termite lipase powder and 4% by mass of termites protease, at a temperature of 45 8 hours under the conditions of hydrolysis degree, enzymatic liquid discharged from the extraction tank through a 100 mesh filter at the beginning, and then filtered through a centrifuge, the filtrate is collected termites extract standby; (2) process and the choice of resin type selection ZG S 820 macroporous resin, the resin is charged in a resin tank, first with water and washed with a flow rate of 2BV 2BV / h, and then 2BV mass concentration of 4% sodium hydroxide solution at 6 BV / h flow rate through the resin, to continue with IBV mass concentration of 2% sodium hydroxide solution at 4 BV / h flow rate through the resin, and finally with 0.5V mass concentration of 1% sodium hydroxide solution at 2 BV / h flow rate through the resin, the standby; (3) extraction in addition to arsenic ions termites liquid extract prepared in item (1) is passed through the resin flow 速为3 BV/h,树脂与白蚁干品的质量比是1: 1,温度为60°(:,?!1<10, 收集通过树脂的白蚁提取液,经浓缩、干燥后得到白蚁提取物干粉,用于保健品生产。 Speed ​​of 3 BV / h, the mass ratio of the resin termites dried is 1: 1, a temperature of 60 ° (:, 1 <10, collected termites extract is passed through the resin, concentrated, and dried to give termite extract?! dry powder, for health care products.

[0014] 对比例1: 一种用新型树脂吸附去除白蚁提取物中砷的方法,具体步骤为: (1) 白蚁提取液的制备白蚁经清洗、干燥后制得白蚁干品,称重后粉碎过60目筛为白蚁粉,备用, 将粉碎好的白蚁粉加入提取罐,加入7倍质量的纯化水,再加入2%白蚁粉质量的脂肪酶和4%白蚁粉质量的蛋白酶,在温度50度的条件下酶解9小时, 酶解液从提取罐放出经60目初滤,再经离心机过滤,收集滤液即为白蚁提取液,备用; (2) 树脂选用和处理选用ZG S 820型树脂,将树脂装入树脂罐,用2BV的水以2BV/h的流速洗涤,再用2BV 质量浓度为4%的氢氧化钠溶液以3BV/h的流速通过树脂,备用; (3) 提取液除砷离子将(1)项中制备的白蚁提取液通过树脂,流速为4BV/h,树脂与白蚁干品的质量比是1: 1,温度为20°(:4!1<10, 收集通过树脂的白蚁提取液,经浓缩、干燥后得到白蚁提取物干粉,用 [0014] Comparative Example 1: A new type adsorption resin termite extract arsenic removal, specific steps: washing the termites prepared by (1) the extract termites, termites after dried to obtain dried, weighed pulverized 60 mesh powder termites, standby, the pulverized powder into an extraction tank termite good, 7 times the mass of purified water was added, and then 2% by mass of powder termite lipase powder and 4% by mass of termites protease, at a temperature of 50 9 hours under conditions of hydrolysis, the hydrolysis solution was discharged from the initial filter 60 mesh extraction tank, centrifuge and then by filtration, the filtrate is collected termites extract standby; (2) process and the choice of resin type selection ZG S 820 the resin, the resin was charged resin tank, washed with water 2BV flow 2BV / h, and then 2BV mass concentration of 4% sodium hydroxide solution at 3BV / h of flow rate through the resin, the standby; (3) extract in addition to arsenic ions termites extract prepared in item (1) is passed through the resin flow rate of 4BV / h, the mass ratio of the resin termites dried is 1: 1, a temperature of 20 ° (:! 4 1 <10, was collected by termites extract resin, concentrated, and dried to give a dry powder extract termites, with 保健品生产。 Health care products.

[0015] 对比例2: 一种用新型树脂吸附去除白蚁提取物中砷的方法,具体步骤为: (1) 白蚁提取液的制备白蚁经清洗、干燥后制得白蚁干品,称重后粉碎过60目筛为白蚁粉,备用, 将粉碎好的白蚁粉加入提取罐,加入12倍质量的纯化水,再加入2%白蚁粉质量的脂肪酶和4%白蚁粉质量的蛋白酶,在温度50度的条件下酶解5小时, 酶解液从提取罐放出经120目初滤,再经离心机过滤,收集滤液即为白蚁提取液,备用; (2) 树脂选用和处理选用ZG S 820型大孔树脂,将树脂装入树脂罐,先用2BV的水以2BV/h的流速洗涤,再用2BV质量浓度为4%的氢氧化钠溶液以7BV/h的流速通过树脂,继续用IBV质量浓度为2%的氢氧化钠溶液以lBV/h的流速通过树脂,最后用0.5V质量浓度为1%的氢氧化钠溶液以3BV/ h的流速通过树脂,备用; (3) 提取液除砷离子将(1)项中制备的白蚁提取液通过树脂,流速 [0015] Comparative Example 2: Adsorption one kind of a novel termite extract arsenic removal, specific steps: washing the termites prepared by (1) the extract termites, termites after dried to obtain dried, weighed pulverized 60 mesh powder termites, standby, pulverized powder is added termite good extraction tank, purified water was added to 12 times the mass of powder was added 2% by mass termite lipase powder and 4% by mass of termites protease, at a temperature of 50 hydrolysis under conditions of 5 hours, the enzyme was released from the extraction tank through a 120 mesh filter at the beginning, and then filtered through a centrifuge, the filtrate is collected termites extract standby; (2) process and the choice of resin type selection ZG S 820 macroporous resin, the resin is charged in a resin tank, first with water and washed with a flow rate of 2BV 2BV / h, and then 2BV mass concentration of 4% sodium hydroxide solution 7BV / h of flow rate through the resin mass continues with IBV concentration of 2% sodium hydroxide solution lBV / h of flow rate through the resin, and finally with 0.5V mass concentration of 1% sodium hydroxide solution at 3BV / h of flow rate through the resin, the standby; (3) extract arsenic removal termites extract ions prepared in (1) item by resin, the flow rate 为lBV/h,树脂与白蚁干品的质量比是1: 1,温度为70°(:4!1<10, 收集通过树脂的白蚁提取液,经浓缩、干燥后得到白蚁提取物干粉,用于保健品生产。 Is lBV / h, the mass ratio of the resin termites dried is 1: 1, a temperature of 70 ° (:! 4 1 <10, collected termites extract is passed through the resin, concentrated, and dried to give termites extract powder, with in health care products.

[0016] 对比例3: 一种用新型树脂吸附去除白蚁提取物中砷的方法,具体步骤为: 树脂选用ZG FS 821型树脂,将树脂装入树脂罐,其余同实施例5。 [0016] Comparative Example 3: A method for termites extract arsenic adsorbed and removed with new resin, specific steps: a resin selected ZG FS 821 resin, the resin tank loaded resin remaining in Example 5.

[0017] 对比例4: 一种用新型树脂吸附去除白蚁提取物中砷的方法,具体步骤为: 白蚁提取液的制备中:仅加入2%白蚁粉质量的脂肪酶,其余同实施例2。 [0017] Comparative Example 4: Novel resin with one kind of termite extract arsenic adsorbed and removed, the specific steps: Preparation of extract of termites: only 2% of the mass of powder lipase termites, and the rest with Example 2.

[0018] 以下表格为各个实施例及对比例制得的白蚁提取物中砷的含量,去除率=(原白蚁提取物中砷含量-各个实施例及对比例制得的白蚁提取物中砷含量)/原白蚁提取物中砷含量X 100%。 [0018] The following table shows the various embodiments and a content ratio of the obtained extracts termites arsenic removal rate = (original extract termites arsenic content - and the proportion of the various embodiments of termites obtained extract arsenic content of ) / original extract termites arsenic content X 100%.

Figure CN105617715AD00081

[0019]从上表的数据可知,先把实施例和对比例比较:本发明的实施例1-6制得的白蚁提取物中砷的含量,明显低于对比例1及2(在本发明工艺参数范围之外)、对比例3(采用另一种ZG FS 821型树脂吸附)、对比例4(只采用一种酶预处理白蚁);再把实施例内部比较:实施例4-6(用氢氧化钠溶液梯度处理树脂)制得的白蚁提取物中砷的含量,明显低于实施例1_3(只用一种浓度的氢氧化钠溶液梯度处理树脂)制得的白蚁提取物中砷的含量,尤其是以实施例5的效果最好;可见本发明先采用复合酶处理白蚁,选用特定的树脂,再用氢氧化钠溶液梯度处理树脂,限定工艺参数,大大降低了白蚁提取物中砷的含量,使其能作为保健食品的原料,符合保健食品的标准。 [0019] apparent from the data in the table, Comparative Examples and first comparison: Content Examples 1-6 of the present invention was prepared termites extract arsenic, significantly lower than the Comparative Example 1 and 2 (in the present invention, outside the range of process parameters), Comparative Example 3 (using another ZG FS 821 adsorption resin), Comparative Example 4 (using only one enzyme pretreatment termite); then the Comparative Example embodiment internal: Example 4-6 ( processing content of the resulting extract termites arsenic resin) with sodium hydroxide solution gradient, significantly lower than the extract of termites obtained in Example 1_3 arsenic embodiment (only the resin was treated with sodium hydroxide solution having a concentration gradient) is content, in particular in Example 5, the effect of the best embodiment; seen that the present invention is first composite termite enzyme treatment, the choice of the particular resin, the resin was treated with caustic soda solution gradient, defined process parameters greatly reduces the termites extract arsenic the content, it can be used as a raw material of health food, health food standard.

Claims (3)

  1. 1. 一种用新型树脂吸附去除白蚁提取物中砷的方法,其特征为:步骤为:(1)白蚁提取液的制备;(2)树脂选用和处理;(3)提取液除砷离子。 A new type resin termite extract arsenic adsorbed and removed, characterized in that: the steps of: (1) Preparation of termites extract; (2) process and choice of resin; (3) In addition to extract arsenic ions.
  2. 2. -种用新型树脂吸附去除白蚁提取物中砷的方法,其特征为:具体步骤为: (1) 白蚁提取液的制备白蚁经清洗、干燥后制得白蚁干品,称重后粉碎过60目筛为白蚁粉,备用, 将粉碎好的白蚁粉加入提取罐,加入8-10倍质量的纯化水,再加入2%白蚁粉质量的脂肪酶和4%白蚁粉质量的蛋白酶,在温度40-45度的条件下酶解6-8小时, 酶解液从提取罐放出经80-100目初滤,再经离心机过滤,收集滤液即为白蚁提取液,备用; (2) 树脂选用和处理选用ZG S 820型树脂,将树脂装入树脂罐,用2BV的水以2BV/h的流速洗涤,再用2BV 质量浓度为4%的氢氧化钠溶液以4-6 BV/h的流速通过树脂,备用; (3) 提取液除砷离子将(1)项中制备的白蚁提取液通过树脂,流速为2-3 BV/h,树脂与白蚁干品的质量比是1:1,温度为25°C-60°C,pH<10, 收集通过树脂的白蚁提取液,经浓缩、干燥后得到白蚁提取 2. - termite species extract arsenic removal with new resin adsorption, wherein: the specific steps: Preparation of the (1) termites termites extract was washed and dried to obtain termites dried, weighed pulverized 60 mesh sieve powder termites, standby, pulverized powder is added termite good extraction tank, purified water was added 8-10 times the mass of powder was added 2% by mass termite lipase powder and 4% by mass of termites protease, at a temperature enzymatic 6-8 hours at 40-45 degrees, enzymatic liquid discharged from the filter through 80-100 mesh First extraction tank, centrifuge and then by filtration, the filtrate is collected termites extract standby; (2) a resin selected and selection processing ZG S 820 resin, the resin was charged resin tank, washed with water 2BV flow 2BV / h, and then 2BV mass concentration of 4% sodium hydroxide solution at 4-6 BV / h flow rate (3) in addition to extract arsenic ions termites extract prepared in item (1) is passed through the resin flow rate of 2-3 BV / h, the mass ratio of the resin is dried termites 1; a resin, a spare: 1, temperature of 25 ° C-60 ° C, pH <10, termites collected through a resin extract, concentrated, and dried to give extract termites 物干粉,用于保健品生产。 Dry powder, for health care products.
  3. 3. -种用新型树脂吸附去除白蚁提取物中砷的方法,其特征为:具体步骤为: (1) 白蚁提取液的制备白蚁经清洗、干燥后制得白蚁干品,称重后粉碎过60目筛为白蚁粉,备用, 将粉碎好的白蚁粉加入提取罐,加入8-10倍质量的纯化水,再加入2%白蚁粉质量的脂肪酶和4%白蚁粉质量的蛋白酶,在温度40-45度的条件下酶解6-8小时, 酶解液从提取罐放出经80-100目初滤,再经离心机过滤,收集滤液即为白蚁提取液,备用; (2) 树脂选用和处理选用ZG S 820型大孔树脂,将树脂装入树脂罐,先用2BV的水以2BV/h的流速洗涤,再用2BV质量浓度为4%的氢氧化钠溶液以4-6 BV/h的流速通过树脂,继续用1BV质量浓度为2%的氢氧化钠溶液以2-4 BV/h的流速通过树脂,最后用0.5V质量浓度为1%的氢氧化钠溶液以1-2 BV/h的流速通过树脂,备用; (3) 提取液除砷离子将(1)项中制备的白蚁提取液通过 3. - termite species extracts arsenic removal with new resin adsorption, wherein: the specific steps: Preparation of the (1) termites termites extract was washed and dried to obtain termites dried, weighed pulverized 60 mesh sieve powder termites, standby, pulverized powder is added termite good extraction tank, purified water was added 8-10 times the mass of powder was added 2% by mass termite lipase powder and 4% by mass of termites protease, at a temperature enzymatic 6-8 hours at 40-45 degrees, enzymatic liquid discharged from the filter through 80-100 mesh First extraction tank, centrifuge and then by filtration, the filtrate is collected termites extract standby; (2) a resin selected and selection processing ZG S 820 macroporous resin, the resin is charged in a resin tank, first with water, washed with a flow rate of 2BV 2BV / h, and then 2BV mass concentration of 4% sodium hydroxide solution at 4-6 BV / h flow rate through the resin, continue to use 1BV mass concentration of 2% sodium hydroxide solution at 2-4 BV / h flow rate through the resin, and finally with 0.5V mass concentration of 1% sodium hydroxide solution at 1-2 BV / h of flow rate through the resin, the standby; (3) in addition to extract termites extract the arsenic ions item (1) prepared by 脂,流速为2-3 BV/h,树脂与白蚁干品的质量比是1:1,温度为25°C-60°C,pH<10, 收集通过树脂的白蚁提取液,经浓缩、干燥后得到白蚁提取物干粉,用于保健品生产。 Fat, a flow rate of 2-3 BV / h, the mass ratio of the resin is dried termites 1: 1, a temperature of 25 ° C-60 ° C, pH <10, the resin extract termites collected, dried and concentrated, and dried after the obtained dry extract termites, for the production of health care products.
CN 201610027703 2016-01-18 2016-01-18 Method for removing arsenic from termite extracts by virtue of adsorption of novel resin CN105617715A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1605570A (en) * 2003-10-09 2005-04-13 董军兴 Method for removing arsenic in underground water utilizing metal carried chelating resin
US20060027493A1 (en) * 2001-09-10 2006-02-09 France Paul Amaat Raymond G Process for treating lipophilic fluid
CN101069698A (en) * 2006-05-12 2007-11-14 江西中医学院 Method for preparing termite fugus gardens extract and use thereof
CN103505906A (en) * 2013-09-30 2014-01-15 正大青春宝药业有限公司 Method for reducing arsenic salt load in water extract of traditional Chinese medicine
CN104546926A (en) * 2015-02-06 2015-04-29 江西中天医药生物有限公司 Termites tea and its preparation method
CN204319833U (en) * 2014-11-12 2015-05-13 成都彼斯特生物科技有限公司 Macroporous resin adsorption separation device

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060027493A1 (en) * 2001-09-10 2006-02-09 France Paul Amaat Raymond G Process for treating lipophilic fluid
CN1605570A (en) * 2003-10-09 2005-04-13 董军兴 Method for removing arsenic in underground water utilizing metal carried chelating resin
CN101069698A (en) * 2006-05-12 2007-11-14 江西中医学院 Method for preparing termite fugus gardens extract and use thereof
CN103505906A (en) * 2013-09-30 2014-01-15 正大青春宝药业有限公司 Method for reducing arsenic salt load in water extract of traditional Chinese medicine
CN204319833U (en) * 2014-11-12 2015-05-13 成都彼斯特生物科技有限公司 Macroporous resin adsorption separation device
CN104546926A (en) * 2015-02-06 2015-04-29 江西中天医药生物有限公司 Termites tea and its preparation method

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