CN105586252A - Fermentation liquid transplantation distributor - Google Patents
Fermentation liquid transplantation distributor Download PDFInfo
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- CN105586252A CN105586252A CN201610077612.XA CN201610077612A CN105586252A CN 105586252 A CN105586252 A CN 105586252A CN 201610077612 A CN201610077612 A CN 201610077612A CN 105586252 A CN105586252 A CN 105586252A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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Abstract
The invention discloses a fermentation liquid transplantation distributor. A seed tank fermentation liquid is transferred to the next stage of fermentation tank. The fermentation liquid transplantation distributor comprises a collection pipe, at least one feed pipe, at least one discharge pipe, a drainage pipe, and at least one feed valve, at least one discharge valve and a drainage valve which are respectively correspondingly arranged on the at least one feed pipe, at least one discharge pipe and drainage pipe, wherein the head and tail ends of the collection pipe are respectively led outwards to the drainage pipe; one end of each feed pipe is connected with a seed tank, and the other end is bent upwards and connected with the collection pipe; one end of each discharge pipe is connected with the lower stage of fermentation tank; and the other end is led out upwards and bent, then led out upwards or downwards, and connected with the collection pipe. When the distributor is subjected to steam sterilization, the steam is used for separately sterilizing any feed pipe or discharge pipe or sterilizing the whole distributor. The distributor is simple in structure; and the introduced steam can be used for simultaneously or separately sterilizing the pipelines in the distributor under the condition of not using the exhaust valve, thereby lowering the possibility of microbiological contamination again and ensuring the smoothness of the fermentation operation.
Description
Technical field
The present invention relates to a kind of distributor, be specifically related to a kind of zymotic fluid culture transferring distributor, belong to zymotechnique pipe arrangement.
Background technology
In sweat, often need multistage fermentation tank to carry out the production task of shared fermented product, every grade of fermentation tank is ganged up each other, and every grade of fermentation tank at least more than one, between different fermentations tank, utilize pipeline to connect, can make zymotic fluid move on to subordinate's fermentation tank by upper level fermentation tank.
In sweat, need to avoid zymotic fluid microbiological contamination, if microbiological contamination, the generation of the direct shadow product of meeting, even can cause the failure of fermentation, so each link contacting with process stream needs to be rigid in checking up, makes the probability of microbiological contamination drop to minimum.
In actual production; often meeting is at dead angle or the halfway local drain tap that increases of sterilizing of easily microbiological contamination; make Zymolysis Equipment and pipeline can sterilizing thorough; if but air bleeding valve improper use or self seal are inadequate; also can there is the possibility of microbiological contamination, so should reduce the use of air bleeding valve in the situation that rationally air bleeding valve being set as far as possible.
Summary of the invention
Technical problem to be solved by this invention is, overcome the shortcoming of prior art, a kind of zymotic fluid culture transferring distributor is provided, this distributor is simple in structure, in the time that zymotic fluid is moved to subordinate's fermentation tank from seeding tank, by in the situation that not using air bleeding valve, the steam that passes into can be carried out simultaneously or independent sterilizing and reduce the possibility of microbiological contamination again the pipeline in distributor, ensure carrying out smoothly of fermentation work.
In order to solve above technical problem, the invention provides a kind of zymotic fluid culture transferring distributor, for seeding tank zymotic fluid is moved to subordinate's fermentation tank, comprise gathering tube, at least one feed pipe, at least one discharge nozzle, blow-off pipe and be correspondingly respectively arranged on inlet valve, outlet valve and the blowoff valve on feed pipe, discharge nozzle and blow-off pipe, wherein:
Blow-off pipe is vertically drawn respectively at gathering tube head and the tail two ends downwards, and blowoff valve is set on blow-off pipe; Feed pipe one end is connected with gathering tube and draws straight up, on the feed pipe of the section of drawing, inlet valve is set straight up, and the feed pipe other end connects seeding tank; Discharge nozzle one end is connected with gathering tube and draws straight up, on the discharge nozzle of the section of drawing, outlet valve is set straight up, and the discharge nozzle other end connects subordinate's fermentation tank;
When this distributor carries out steam sterilizing, steam enters distributor by feed pipe or discharge nozzle, by the flow direction of inlet valve and outlet valve control steam, steam is during to the independent sterilizing of feed pipe, open the blowoff valve on inlet valve and the blow-off pipe on feed pipe, close outlet valve simultaneously, complete the independent sterilizing of steam to feed pipe;
Steam during to the independent sterilizing of discharge nozzle, is opened the blowoff valve on outlet valve and the blow-off pipe on discharge nozzle, closes inlet valve simultaneously, completes the independent sterilizing of steam to discharge nozzle;
Steam during to whole distributor sterilizing, is opened inlet valve, outlet valve and blowoff valve simultaneously, completes the sterilizing of steam to whole distributor;
Steam is to arbitrary feed pipe or discharge nozzle carries out independent sterilizing or to whole distributor sterilizing, the steam condensate (SC) that sterilizing produces is discharged by described blow-off pipe.
Technique effect, the structure of distributor of the present invention, do not use in the situation of air bleeding valve, can be by the flow direction of the keying control steam of valve in distributor, thereby reach all pipelines sterilizing simultaneously in distributor, also can carry out independent sterilizing to arbitrary feed pipe or discharge nozzle, variation is selected in sterilizing, the needs of more pressing close to fermentation are more humane, reduce number of times and the cost of sterilizing simultaneously.
The technical scheme that the present invention further limits is:
Further, in aforementioned zymotic fluid culture transferring distributor, the feed pipe straight up section of drawing arranges the laggard line bend of inlet valve, and the feed pipe after bending is drawn up or down and is connected with seeding tank; The discharge nozzle straight up section of drawing arranges the laggard line bend of outlet valve, and the discharge nozzle after bending is drawn up or down with subordinate fermentation tank again and is connected.
In aforementioned zymotic fluid culture transferring distributor, between the blow-off pipe at gathering tube head and the tail two ends, the end, is concordant.
Technique effect, blow-off pipe is vertically drawn at gathering tube head and the tail two ends downwards, and between blow-off pipe the end concordant, straight down and concordant can smoothly the steam condensate (SC) after sterilizing the discharge smoothly in the end, utilize the flow direction and the weight of condensed water self to discharge smoothly, can, because of there being angle of inclination problem, not stop the discharge of steam condensate (SC), avoid unclean because of steam condensate (SC) discharge, what impact was fermented carries out smoothly.
In aforementioned zymotic fluid culture transferring distributor, inlet valve and outlet valve next-door neighbour gathering tube.
Technique effect, as outlet valve and inlet valve be arranged on discharge nozzle and feed pipe not straight up the section of drawing be not close to gathering tube yet, and at other position, because too early valve control meeting causes the delay of zymotic fluid or steam, cause utilizing the speed of fluid self to flow into smoothly gathering tube, can not flow into smoothly subordinate's fermentation tank, this set is placed does not need extra circulation aid, just can carry out smoothly the distribution of zymotic fluid and the sterilizing of steam, simple, with low cost.
In aforementioned zymotic fluid culture transferring distributor, apply one deck overcoat in feed pipe, described overcoat comprises following component according to the mass fraction:
Bisphenol f type epoxy resin: 8-10 part, bisphenol A type epoxy resin: 20-30 part, barium sulfate: 5-7 part, three silica powders: 1-3 part, titanium oxide: 1-2 part, fatty amine: 10-13 part, antiseptic: 6-8 part, compound rare-earth: 0.2-0.5 part;
Compound rare-earth comprises following component: La:10-15% according to the mass fraction, Y:16-18%, and Sc:10-15%, Gd:8-10%, Sm:10-13%, Pr:8-12%, all the other are lanthanide series, above each component sum is 100%;
The preparation technology of overcoat is:
Bisphenol f type epoxy resin and bisphenol A type epoxy resin are put in dispersion cylinder, turn on agitator slowly stirs, mixing speed is turn/min of 200-250, stir 15-30min, then, to disperseing to drop into barium sulfate, three silica powders and titanium oxide in cylinder, continue to stir 20-30min, mixing speed is turn/min of 150-200, then under stirring, add antiseptic and compound rare-earth, continue to stir 30-40min, mixing speed is that turn/min of 100-120 obtains compound;
Compound is mixed with fatty amine, then overlay on the inside of feed pipe, at room temperature solidify and can obtain overcoat.
In aforementioned zymotic fluid culture transferring distributor, apply layer protective layer in discharge nozzle, described protective layer comprises following component according to the mass fraction:
Modified epoxy: 20-30 part, acrylic resin: 10-15 part, lauryl alcohol ester: 5-8 part, aluminate coupling agent: 10-15 part, dicyandiamide: 1-3 part, titanium dioxide: 1-1.5 part, zinc oxide: 1-1.5 part, antiseptic: 5-7 part;
The preparation technology of protective layer is:
Modified epoxy and acrylic resin are mixed; then adding lauryl alcohol ester to regulate mixing speed is turn/min of 100-150; stir 15-30min; then add dicyandiamide, titanium dioxide, zinc oxide; continue to stir 12-15min; mixing speed is turn/min of 150-180; under stirring, add aluminate coupling agent and antiseptic; regulating mixing speed is turn/min of 200-250; stir 20-30min; material after stirring is coated on the inwall of discharge nozzle, cold curing can obtain protective layer.
In aforementioned zymotic fluid culture transferring distributor, the concrete preparation method of antiseptic is:
Under room temperature, the absolute ethyl alcohol that accounts for total amount 2/3 is mixed with butyl titanate, on magnetic stirring apparatus, stir 20-30min and obtain mixed solution A, again the absolute ethyl alcohol of residue 1/3 amount is mixed with nitric acid and obtains mixed solution B, mixed solution B is slowly added drop-wise in mixed solution A, and rate of addition is controlled at 1-2 drop/sec, and stir on dropping limit, limit, after being added dropwise to complete, on magnetic stirring apparatus, continue to stir 10-20min, make flaxen transparent titanium colloidal sol;
In there-necked flask, prepare montmorillonite suspension, suspension is placed in to thermostat water bath, maintain the temperature at 37 DEG C, the titanium colloidal sol making is slowly added dropwise to wherein, stir on dropping limit, limit, make mixed liquor C, drip rear continuation and stir 1-2h, after ageing 24h under room temperature, form the dispersed TiO wherein of montmorillonite2Gel, by gel washing, centrifugal dehydration, dry at 60-70 DEG C, then deliver to high temperature kiln roasting, controlling heating rate is 5-7 DEG C/min, is warming up to 150-170 DEG C, then constant temperature 30-50min is cooled to after room temperature fine groundly, can obtain antiseptic.
The invention has the beneficial effects as follows:
Feed pipe of the present invention one end is connected with gathering tube, and draw straight up, in the section of drawing, inlet valve is set straight up at feed pipe, and inlet valve next-door neighbour gathering tube, same discharge nozzle is also that one end is connected with gathering tube, then draw straight up, in the section of drawing, outlet valve is set straight up at discharge nozzle, and outlet valve next-door neighbour gathering tube, such setting does not need extra use air bleeding valve to carry out exhaust sterilizing, because knowing from experience, vapor stream utilize the fluid velocity of self and gravity to flow into smoothly gathering tube, flow into smoothly subordinate's fermentation tank from gathering tube again, air bleeding valve just need to be set because vapor stream will overcome self gravitation and flow into gathering tube if arranged straight down, easily there is the phenomenons such as steam discharge is incomplete, use air bleeding valve easily occur improper use or self seal inadequate, there is the possibility of microbiological contamination, so should reduce the use of air bleeding valve in the situation that rationally air bleeding valve being set as far as possible, the present invention has stopped the use of air bleeding valve completely, reduce the possibility of microbiological contamination again, ensure carrying out smoothly of fermentation work.
In the present invention, can pass into steam from any part of distributor and carry out sterilizing, by the keying of outlet valve and inlet valve, can carry out steam sterilizing to arbitrary feed pipe or discharge nozzle or whole device, the condensed water producing in steam sterilizing process can be discharged smoothly by blow-off pipe, there is not the halfway dead angle of sterilizing, without increasing in addition air bleeding valve, greatly reduce zymotic fluid and move the probability of material pipeline microbiological contamination, be applicable to large-scale popularization, and with low cost.
In discharge nozzle and feed pipe, applying overcoat and protective layer polishes discharge nozzle and feed pipe; can reduce mill resistance; reduce kinetic equation loss; displacement and sterilizing speed while improving steam sterilizing; inner protective layer or overcoat can reduce the pollution of medium, omit the needed facility of a lot of cleaning pipeline, reduce maintenance and expense of labor; in the service life that has extended pipeline, reduce costs.
Taking the composite of nano titanium oxide and montmorillonite as antiseptic, utilize the photocatalysis performance of titanium dioxide, play antibacterial, deodorizing, the effect such as purify air, nanometer titanium dioxide antibiotic, the possibility that can reduce cross-infection and contact infection, prevents biological pollution etc., improves the quality of products.
In the time preparing antiseptic, ethanol is a kind of good organic solvent, and boiling point is lower, is conducive to the discharge of solvent in heating process; In the present invention, add acid as catalyst, can not only slow down the hydrolysis reaction of alkoxide, the polymerization rate of alkoxide can slow down simultaneously, on the one hand, in solution, exist H+ to make hydrolysis counter movement, suppress hydrolysis, hydrolysis is by slack-off, the speed of the polymerisation that slowed down, stops micelle cohesion.
Antiseptic in the present invention is the composite of titanium dioxide/montmorillonite, using montmorillonite as carrier, for the load of titanium dioxide provides huge surface area, make the titanium dioxide nano-particle generating can be evenly dispersed in surface and the interlayer of montmorillonite, stop titanium dioxide nano-particle to be reunited on the one hand, also increase on the other hand the contact probability of titanium dioxide and azo dyes, improved quantum utilization ratio. Titanium dioxide has good whiteness and covering power, surface-treated titanium dioxide has the ageing resistace of improving coating, extend the effect in coating life-span, this raw material as a kind of coating of montmorillonite, utilizes colloid dispersiveness, thickening property, caking property, thixotropy and suspension stability that bentonite is good, can improve the performance of coating, the antiseptic that both are compound, by comprehensive both advantage, make up the other side's shortcoming, improve the performance of coating.
Brief description of the drawings
Fig. 1 is the workflow diagram of zymotic fluid culture transferring distributor of the present invention;
In figure: 1-gathering tube, 2-feed pipe, 3-discharge nozzle, 4-blow-off pipe, 5-inlet valve, 6-outlet valve, 7-blowoff valve.
Detailed description of the invention
Embodiment 1
A kind of zymotic fluid culture transferring distributor that the present embodiment provides, structure as shown in Figure 1, for seeding tank zymotic fluid is moved to subordinate's fermentation tank, the mouth of pipe of seeding tank delivery outlet and fermentation tank input port is towards identical, comprise gathering tube 1, at least one feed pipe 2, at least one discharge nozzle 3, blow-off pipe 4 and be correspondingly respectively arranged on inlet valve 5, outlet valve 6 and the blowoff valve 7 on feed pipe 2, discharge nozzle 3 and blow-off pipe 4, inlet valve 5 and outlet valve 6 are close to gathering tube 1, wherein:
Blow-off pipe 4 is vertically drawn respectively at gathering tube 1 head and the tail two ends downwards, between blow-off pipe 4 end concordant, blowoff valve 7 is set on blow-off pipe 4; Feed pipe 2 one end are connected with gathering tube 1 and draw straight up, on the feed pipe 2 of the section of drawing, inlet valve 5 are set straight up, and feed pipe 2 other ends connect seeding tank; Discharge nozzle 3 one end are connected with gathering tube 1 and draw straight up, on the discharge nozzle 3 of the section of drawing, outlet valve 6 are set straight up, and discharge nozzle 3 other ends connect subordinate's fermentation tank;
When this distributor carries out steam sterilizing, steam enters distributor by feed pipe 2 or discharge nozzle 3, control the flow direction of steam by inlet valve 5 and outlet valve 6, steam is during to feed pipe 2 independent sterilizing, open the blowoff valve 7 on inlet valve 5 and the blow-off pipe 4 on feed pipe 2, close outlet valve 6 simultaneously, complete the independent sterilizing of steam to feed pipe 2;
Steam during to discharge nozzle 3 independent sterilizing, is opened the blowoff valve 7 on outlet valve 6 and the blow-off pipe 4 on discharge nozzle 3, closes inlet valve 5 simultaneously, completes the independent sterilizing of steam to discharge nozzle 3;
Steam during to whole distributor sterilizing, is opened inlet valve 5, outlet valve 6 and blowoff valve 7 simultaneously, completes the sterilizing of steam to whole distributor;
Steam is to arbitrary feed pipe 2 or discharge nozzle 3 carries out independent sterilizing or to whole distributor sterilizing, the steam condensate (SC) that sterilizing produces is discharged by blow-off pipe 4.
In the present embodiment: the feed pipe 2 straight up section of drawing arranges after inlet valve 5 and carries out bending, and the feed pipe 2 after bending is drawn up or down and is connected with seeding tank; The discharge nozzle 3 straight up section of drawing arranges the laggard line bend of outlet valve 6, and the discharge nozzle 3 after bending is drawn up or down with subordinate fermentation tank again and is connected.
Embodiment 2
The present embodiment provides in above-described embodiment 1 and in feed pipe, applies the protective layer applying in overcoat and discharge nozzle, wherein:
Overcoat comprises following component according to the mass fraction:
Bisphenol f type epoxy resin: 8 parts, bisphenol A type epoxy resin: 20 parts, barium sulfate: 5 parts, three silica powders: 1 part, titanium oxide: 1 part, fatty amine: 10 parts, antiseptic: 6 parts, compound rare-earth: 0.2 part;
Compound rare-earth comprises following component: La:10% according to the mass fraction, Y:16%, and Sc:10%, Gd:8%, Sm:10%, Pr:8%, all the other are lanthanide series, above each component sum is 100%;
The preparation technology of overcoat is:
Bisphenol f type epoxy resin and bisphenol A type epoxy resin are put in dispersion cylinder, turn on agitator slowly stirs, mixing speed is 200 turn/min, stir 15min, then, to disperseing to drop into barium sulfate, three silica powders and titanium oxide in cylinder, continue to stir 20min, mixing speed is 150 turn/min, then under stirring, add antiseptic and compound rare-earth, continue to stir 30min, mixing speed is that 100 turn/min obtain compound;
Compound is mixed with fatty amine, then overlay on the inside of feed pipe, at room temperature solidify and can obtain overcoat.
Protective layer comprises following component according to the mass fraction:
Modified epoxy: 20 parts, acrylic resin: 10 parts, lauryl alcohol ester: 5 parts, aluminate coupling agent: 10 parts, dicyandiamide: 1 part, titanium dioxide: 1 part, zinc oxide: 1 part, antiseptic: 5 parts;
The preparation technology of protective layer is:
Modified epoxy and acrylic resin are mixed; then adding lauryl alcohol ester to regulate mixing speed is 100 turn/min; stir 15min, then add dicyandiamide, titanium dioxide, zinc oxide, continue to stir 12min; mixing speed is 150 turn/min; under stirring, add aluminate coupling agent and antiseptic, regulating mixing speed is 200 turn/min, stirs 20min; material after stirring is coated on the inwall of discharge nozzle, cold curing can obtain protective layer.
In overcoat and protective layer, the concrete preparation method of antiseptic is:
Under room temperature, the absolute ethyl alcohol that accounts for total amount 2/3 is mixed with butyl titanate, on magnetic stirring apparatus, stir 20min and obtain mixed solution A, again the absolute ethyl alcohol of residue 1/3 amount is mixed with nitric acid and obtains mixed solution B, mixed solution B is slowly added drop-wise in mixed solution A, and rate of addition is controlled at 1 drop/sec, and stir on dropping limit, limit, after being added dropwise to complete, on magnetic stirring apparatus, continue to stir 10min, make flaxen transparent titanium colloidal sol;
In there-necked flask, prepare montmorillonite suspension, suspension is placed in to thermostat water bath, maintain the temperature at 37 DEG C, the titanium colloidal sol making is slowly added dropwise to wherein, stir on dropping limit, limit, make mixed liquor C, drip rear continuation and stir 1h, after ageing 24h under room temperature, form the dispersed TiO wherein of montmorillonite2Gel, by gel washing, centrifugal dehydration, dry at 60 DEG C, then deliver to high temperature kiln roasting, controlling heating rate is 5 DEG C/min, is warming up to 150 DEG C, then constant temperature 30min is cooled to after room temperature fine groundly, can obtain antiseptic.
Embodiment 3
The present embodiment provides in above-described embodiment 1 and in feed pipe, applies the protective layer applying in overcoat and discharge nozzle, wherein:
Overcoat comprises following component according to the mass fraction:
Bisphenol f type epoxy resin: 10 parts, bisphenol A type epoxy resin: 30 parts, barium sulfate: 7 parts, three silica powders: 3 parts, titanium oxide: 2 parts, fatty amine: 13 parts, antiseptic: 8 parts, compound rare-earth: 0.5 part;
Compound rare-earth comprises following component: La:15% according to the mass fraction, Y:18%, and Sc:15%, Gd:10%, Sm:13%, Pr:12%, all the other are lanthanide series, above each component sum is 100%;
The preparation technology of overcoat is:
Bisphenol f type epoxy resin and bisphenol A type epoxy resin are put in dispersion cylinder, turn on agitator slowly stirs, mixing speed is 250 turn/min, stir 30min, then, to disperseing to drop into barium sulfate, three silica powders and titanium oxide in cylinder, continue to stir 30min, mixing speed is 200 turn/min, then under stirring, add antiseptic and compound rare-earth, continue to stir 40min, mixing speed is that 120 turn/min obtain compound;
Compound is mixed with fatty amine, then overlay on the inside of feed pipe, at room temperature solidify and can obtain overcoat.
Protective layer comprises following component according to the mass fraction:
Modified epoxy: 30 parts, acrylic resin: 15 parts, lauryl alcohol ester: 8 parts, aluminate coupling agent: 15 parts, dicyandiamide: 3 parts, titanium dioxide: 1.5 parts, zinc oxide: 1.5 parts, antiseptic: 7 parts;
The preparation technology of protective layer is:
Modified epoxy and acrylic resin are mixed; then adding lauryl alcohol ester to regulate mixing speed is 150 turn/min; stir 30min, then add dicyandiamide, titanium dioxide, zinc oxide, continue to stir 15min; mixing speed is 180 turn/min; under stirring, add aluminate coupling agent and antiseptic, regulating mixing speed is 250 turn/min, stirs 30min; material after stirring is coated on the inwall of discharge nozzle, cold curing can obtain protective layer.
In overcoat and protective layer, the concrete preparation method of antiseptic is:
Under room temperature, the absolute ethyl alcohol that accounts for total amount 2/3 is mixed with butyl titanate, on magnetic stirring apparatus, stir 30min and obtain mixed solution A, again the absolute ethyl alcohol of residue 1/3 amount is mixed with nitric acid and obtains mixed solution B, mixed solution B is slowly added drop-wise in mixed solution A, and rate of addition is controlled at 2 drops/sec, and stir on dropping limit, limit, after being added dropwise to complete, on magnetic stirring apparatus, continue to stir 20min, make flaxen transparent titanium colloidal sol;
In there-necked flask, prepare montmorillonite suspension, suspension is placed in to thermostat water bath, maintain the temperature at 37 DEG C, the titanium colloidal sol making is slowly added dropwise to wherein, stir on dropping limit, limit, make mixed liquor C, drip rear continuation and stir 2h, after ageing 24h under room temperature, form the dispersed TiO wherein of montmorillonite2Gel, by gel washing, centrifugal dehydration, dry at 670 DEG C, then deliver to high temperature kiln roasting, controlling heating rate is 7 DEG C/min, is warming up to 170 DEG C, then constant temperature 50min is cooled to after room temperature fine groundly, can obtain antiseptic.
Embodiment 4
The present embodiment provides in above-described embodiment 1 and in feed pipe, applies the protective layer applying in overcoat and discharge nozzle, wherein:
Overcoat comprises following component according to the mass fraction:
Bisphenol f type epoxy resin: 9 parts, bisphenol A type epoxy resin: 25 parts, barium sulfate: 6 parts, three silica powders: 1-3 part, titanium oxide: 1 part, fatty amine: 12 parts, antiseptic: 7 parts, compound rare-earth: 0.3 part;
Compound rare-earth comprises following component: La:12% according to the mass fraction, Y:17%, and Sc:13%, Gd:9%, Sm:11%, Pr:11%, all the other are lanthanide series, above each component sum is 100%;
The preparation technology of overcoat is:
Bisphenol f type epoxy resin and bisphenol A type epoxy resin are put in dispersion cylinder, turn on agitator slowly stirs, mixing speed is 220 turn/min, stir 20min, then, to disperseing to drop into barium sulfate, three silica powders and titanium oxide in cylinder, continue to stir 25min, mixing speed is 180 turn/min, then under stirring, add antiseptic and compound rare-earth, continue to stir 35min, mixing speed is that 110 turn/min obtain compound;
Compound is mixed with fatty amine, then overlay on the inside of feed pipe, at room temperature solidify and can obtain overcoat.
Protective layer comprises following component according to the mass fraction:
Modified epoxy: 25 parts, acrylic resin: 12 parts, lauryl alcohol ester: 6 parts, aluminate coupling agent: 12 parts, dicyandiamide: 2 parts, titanium dioxide: 1.2 parts, zinc oxide: 1.3 parts, antiseptic: 6 parts;
The preparation technology of protective layer is:
Modified epoxy and acrylic resin are mixed; then adding lauryl alcohol ester to regulate mixing speed is 120 turn/min; stir 18min, then add dicyandiamide, titanium dioxide, zinc oxide, continue to stir 13min; mixing speed is 170 turn/min; under stirring, add aluminate coupling agent and antiseptic, regulating mixing speed is 220 turn/min, stirs 25min; material after stirring is coated on the inwall of discharge nozzle, cold curing can obtain protective layer.
In overcoat and protective layer, the concrete preparation method of antiseptic is:
Under room temperature, the absolute ethyl alcohol that accounts for total amount 2/3 is mixed with butyl titanate, on magnetic stirring apparatus, stir 25min and obtain mixed solution A, again the absolute ethyl alcohol of residue 1/3 amount is mixed with nitric acid and obtains mixed solution B, mixed solution B is slowly added drop-wise in mixed solution A, and rate of addition is controlled at 1 drop/sec, and stir on dropping limit, limit, after being added dropwise to complete, on magnetic stirring apparatus, continue to stir 15min, make flaxen transparent titanium colloidal sol;
In there-necked flask, prepare montmorillonite suspension, suspension is placed in to thermostat water bath, maintain the temperature at 37 DEG C, the titanium colloidal sol making is slowly added dropwise to wherein, stir on dropping limit, limit, make mixed liquor C, drip rear continuation and stir 1h, after ageing 24h under room temperature, form the dispersed TiO wherein of montmorillonite2Gel, by gel washing, centrifugal dehydration, dry at 65 DEG C, then deliver to high temperature kiln roasting, controlling heating rate is 6 DEG C/min, is warming up to 160 DEG C, then constant temperature 40min is cooled to after room temperature fine groundly, can obtain antiseptic.
This distributor of the present invention is simple and reasonable, in the time that zymotic fluid is moved to subordinate's fermentation tank from seeding tank, by in the situation that not using air bleeding valve, the steam that passes into can be carried out simultaneously or independent sterilizing and reduce the possibility of microbiological contamination again the pipeline in distributor, ensure carrying out smoothly of fermentation work;
Protective layer and overcoat on discharge nozzle and feed pipe, are applied respectively; can polish; can reduce mill resistance; reduce kinetic equation loss, displacement and sterilizing speed while improving steam sterilizing, inner protective layer or overcoat can reduce the pollution of medium; omit the needed facility of a lot of cleaning pipeline; reduce maintenance and expense of labor, extended the service life of pipeline, reduce costs.
In addition to the implementation, the present invention can also have other embodiments. All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of requirement of the present invention.
Claims (7)
1. a zymotic fluid culture transferring distributor, for seeding tank zymotic fluid is moved to subordinate's fermentation tank, it is characterized in that: comprise gathering tube (1), at least one feed pipe (2), at least one discharge nozzle (3), blow-off pipe (4) and respectively correspondence be arranged on inlet valve (5), outlet valve (6) and the blowoff valve (7) on feed pipe (2), discharge nozzle (3) and blow-off pipe (4), wherein:
Described blow-off pipe (4) is vertically drawn respectively at described gathering tube (1) head and the tail two ends downwards, and described blowoff valve (7) is set on described blow-off pipe (4); Described feed pipe (2) one end is connected with described gathering tube (1) and draws straight up, and described inlet valve (5) is set on the feed pipe of the section of drawing (2) straight up, and feed pipe (2) other end connects seeding tank; Described discharge nozzle (3) one end is connected with described gathering tube (1) and draws straight up, and described outlet valve (6) is set on the discharge nozzle of the section of drawing (3) straight up, and discharge nozzle (3) other end connects subordinate's fermentation tank;
When this distributor carries out steam sterilizing, steam enters distributor by described feed pipe (2) or discharge nozzle (3), control the flow direction of steam by inlet valve (5) and outlet valve (6), steam is to feed pipe (2) separately when sterilizing, open the blowoff valve (7) on inlet valve (5) and the blow-off pipe (4) on feed pipe (2), close outlet valve (6) simultaneously, complete the independent sterilizing of steam to feed pipe (2);
Steam separately when sterilizing, is opened the blowoff valve (7) on outlet valve (6) and the blow-off pipe (4) on discharge nozzle (3) to discharge nozzle (3), closes inlet valve (5) simultaneously, completes the independent sterilizing of steam to discharge nozzle (3);
Steam during to whole distributor sterilizing, is opened inlet valve (5), outlet valve (6) and blowoff valve (7) simultaneously, completes the sterilizing of steam to whole distributor;
Steam is to arbitrary feed pipe (2) or discharge nozzle (3) carries out independent sterilizing or to whole distributor sterilizing, the steam condensate (SC) that sterilizing produces is discharged by described blow-off pipe.
2. zymotic fluid culture transferring distributor according to claim 1, is characterized in that: described feed pipe (2) the straight up section of drawing arranges (5) after inlet valve and carries out bending, and the feed pipe (2) after bending is drawn up or down and is connected with seeding tank; Described discharge nozzle (3) the straight up section of drawing arranges the laggard line bend of outlet valve (6), and the discharge nozzle (3) after bending is drawn up or down with subordinate fermentation tank again and is connected.
3. zymotic fluid culture transferring distributor according to claim 1, is characterized in that: between the blow-off pipe (4) at described gathering tube (1) head and the tail two ends, the end is concordant.
4. zymotic fluid culture transferring distributor according to claim 1, is characterized in that: described inlet valve (5) and the described gathering tube (1) of outlet valve (6) next-door neighbour.
5. according to the zymotic fluid culture transferring distributor described in claim 1-4, it is characterized in that: in described feed pipe, apply one deck overcoat, described overcoat comprises following component according to the mass fraction:
Bisphenol f type epoxy resin: 8-10 part, bisphenol A type epoxy resin: 20-30 part, barium sulfate: 5-7 part, three silica powders: 1-3 part, titanium oxide: 1-2 part, fatty amine: 10-13 part, antiseptic: 6-8 part, compound rare-earth: 0.2-0.5 part;
Described compound rare-earth comprises following component: La:10-15% according to the mass fraction, Y:16-18%, and Sc:10-15%, Gd:8-10%, Sm:10-13%, Pr:8-12%, all the other are lanthanide series, above each component sum is 100%;
The preparation technology of described overcoat is:
Bisphenol f type epoxy resin and bisphenol A type epoxy resin are put in dispersion cylinder, turn on agitator slowly stirs, mixing speed is turn/min of 200-250, stir 15-30min, then, to disperseing to drop into barium sulfate, three silica powders and titanium oxide in cylinder, continue to stir 20-30min, mixing speed is turn/min of 150-200, then under stirring, add antiseptic and compound rare-earth, continue to stir 30-40min, mixing speed is that turn/min of 100-120 obtains compound;
Compound is mixed with fatty amine, then overlay on the inside of feed pipe, at room temperature solidify and can obtain overcoat.
6. according to the zymotic fluid culture transferring distributor described in claim 1-4, it is characterized in that: in described discharge nozzle, apply layer protective layer, described protective layer comprises following component according to the mass fraction:
Modified epoxy: 20-30 part, acrylic resin: 10-15 part, lauryl alcohol ester: 5-8 part, aluminate coupling agent: 10-15 part, dicyandiamide: 1-3 part, titanium dioxide: 1-1.5 part, zinc oxide: 1-1.5 part, antiseptic: 5-7 part;
The preparation technology of described protective layer is:
Modified epoxy and acrylic resin are mixed; then adding lauryl alcohol ester to regulate mixing speed is turn/min of 100-150; stir 15-30min; then add dicyandiamide, titanium dioxide, zinc oxide; continue to stir 12-15min; mixing speed is turn/min of 150-180; under stirring, add aluminate coupling agent and antiseptic; regulating mixing speed is turn/min of 200-250; stir 20-30min; material after stirring is coated on the inwall of discharge nozzle, cold curing can obtain protective layer.
7. according to the zymotic fluid culture transferring distributor described in claim 5 or 6, it is characterized in that: the concrete preparation method of described antiseptic is:
Under room temperature, the absolute ethyl alcohol that accounts for total amount 2/3 is mixed with butyl titanate, on magnetic stirring apparatus, stir 20-30min and obtain mixed solution A, again the absolute ethyl alcohol of residue 1/3 amount is mixed with nitric acid and obtains mixed solution B, mixed solution B is slowly added drop-wise in mixed solution A, and rate of addition is controlled at 1-2 drop/sec, and stir on dropping limit, limit, after being added dropwise to complete, on magnetic stirring apparatus, continue to stir 10-20min, make flaxen transparent titanium colloidal sol;
In there-necked flask, prepare montmorillonite suspension, suspension is placed in to thermostat water bath, maintain the temperature at 37 DEG C, the titanium colloidal sol making is slowly added dropwise to wherein, stir on dropping limit, limit, make mixed liquor C, drip rear continuation and stir 1-2h, after ageing 24h under room temperature, form the dispersed TiO wherein of montmorillonite2Gel, by gel washing, centrifugal dehydration, dry at 60-70 DEG C, then deliver to high temperature kiln roasting, controlling heating rate is 5-7 DEG C/min, is warming up to 150-170 DEG C, then constant temperature 30-50min is cooled to after room temperature fine groundly, can obtain antiseptic.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106190772A (en) * | 2016-07-15 | 2016-12-07 | 江苏尚昆生物设备有限公司 | Material distributing system |
| CN106893672A (en) * | 2017-04-11 | 2017-06-27 | 尹康康 | A kind of soil bacteria stoste takes equipment |
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| WO2004022841A1 (en) * | 2002-09-09 | 2004-03-18 | Jung-Eun Seo | Polymer article and method for manufacturing the same |
| CN202530083U (en) * | 2012-03-29 | 2012-11-14 | 浙江鑫甬生物化工有限公司 | Seed transferring device for fermentation |
| CN203373336U (en) * | 2013-07-17 | 2014-01-01 | 南京朗恩生物科技有限公司 | Sterile feeding device for fermentation tanks |
| CN105062302A (en) * | 2015-07-22 | 2015-11-18 | 安徽圣德建材科技有限公司 | High-performance thermosetting epoxy resin powder coating |
| CN205556666U (en) * | 2016-02-03 | 2016-09-07 | 江苏丰泽生物工程设备制造有限公司 | Zymotic fluid culture transferring distributor |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004022841A1 (en) * | 2002-09-09 | 2004-03-18 | Jung-Eun Seo | Polymer article and method for manufacturing the same |
| CN202530083U (en) * | 2012-03-29 | 2012-11-14 | 浙江鑫甬生物化工有限公司 | Seed transferring device for fermentation |
| CN203373336U (en) * | 2013-07-17 | 2014-01-01 | 南京朗恩生物科技有限公司 | Sterile feeding device for fermentation tanks |
| CN105062302A (en) * | 2015-07-22 | 2015-11-18 | 安徽圣德建材科技有限公司 | High-performance thermosetting epoxy resin powder coating |
| CN205556666U (en) * | 2016-02-03 | 2016-09-07 | 江苏丰泽生物工程设备制造有限公司 | Zymotic fluid culture transferring distributor |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106190772A (en) * | 2016-07-15 | 2016-12-07 | 江苏尚昆生物设备有限公司 | Material distributing system |
| CN106893672A (en) * | 2017-04-11 | 2017-06-27 | 尹康康 | A kind of soil bacteria stoste takes equipment |
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| Publication number | Publication date |
|---|---|
| CN105586252B (en) | 2018-03-09 |
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