CN105580766B - A kind of circulating water cultivation device assessed for aquaculture of aquatic animal and medication - Google Patents
A kind of circulating water cultivation device assessed for aquaculture of aquatic animal and medication Download PDFInfo
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- CN105580766B CN105580766B CN201610134337.0A CN201610134337A CN105580766B CN 105580766 B CN105580766 B CN 105580766B CN 201610134337 A CN201610134337 A CN 201610134337A CN 105580766 B CN105580766 B CN 105580766B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/045—Filters for aquaria
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The present invention relates to a kind of circulating water cultivation devices assessed for aquaculture of aquatic animal and medication.Circulating water cultivation device of the invention combines aquaculture of aquatic animal, water process, water quality monitoring, the cultivation of recirculated water or hydrostatic, cultivation water process and discharge, the detection of cultivation water physical and chemical parameter of achievable fresh water or seawater class aquatic livestock, it can be ensured that (such as vaccine potency evaluation) is assessed in medication, and breeding environment is excellent in the process, experiment condition is controllable.
Description
Technical field
The invention belongs to aquaculture device field, more particularly it relates to it is a kind of for aquaculture of aquatic animal and
The circulating water cultivation device of medication assessment.
Background technique
As people are continuously increased the aquatic resources demand such as fish, high density, intensive culture mode gradually at
For the Developing mainstream of culture fishery.However, the farming disease harms problem becomes increasingly conspicuous while aquaculture rapidly develops.Carefully
The diseases such as bacterium, fungi, virus, helminth have at the whole nation to be broken out, wherein bacterial disease frequency of disease development is high, Epidemic Scope is wide,
Pathogen easily makes a variation, and becomes the important bottleneck of limitation China's culture fishery development.
Currently, based on the prevention and treatment chemically of directed toward bacteria venereal disease evil.Antibiotic is that one kind is widely used in highly dense
Spend the chemical prevention means of cultivation.But the environmental pollution, drug resistant pathogen caused by it are evolved, selection, aquatic products drug are residual
The negative effects such as stay just increasingly to be valued by the people.Therefore, develop new disease control means and have become culture fishery
An urgent demand.
Vaccine inoculation is a kind of novel immune protection technology.However, Chinese Fishery animal vaccine research and development at present are still main
Stay in the laboratory research stage.Experiment multiselect takes clear background, is easy to raise, easy to operate fingerling such as zebra fish, sword tail
Fish etc. carries out vaccine effect evaluation.But the difference of experimental animal and target animals, experimental situation and breeding environment often results in experiment
The deviation of data and clinical data.It can be seen that choosing target animals in experiment is experimental animal, and simulates normal breeding environment and be
Evaluate the best mode of candidate vaccine immune effect.
Industrial circulating water cultivating is that aquaculture batch production develops state-of-the-art production method and China's aquaculture
Important development direction.Compared with traditional breeding way, industrial circulating water cultivating have it is water-saving, save land, high density it is intensive
The features such as controllable with discharge, and normal breeding environment can be simulated, reduce energy consumption by establishing laboratory circulating water culture system not only,
Multinomial experiment parameter such as temperature, salinity, pH etc. can also be regulated and controled.
In conclusion there is an urgent need in the art to develop it is a kind of can be used for aquaculture of aquatic animal and medication assessment laboratory follow
Ring water cultivating system, so that more scientific to the effect evaluation of drug candidate such as vaccine effective.
Summary of the invention
The purpose of the present invention is to provide a kind of circulating water cultivation devices assessed for aquaculture of aquatic animal and medication.
In the first aspect of the present invention, a kind of circulating water cultivation dress assessed for aquaculture of aquatic animal and medication is provided
Set, the device include: culturing jar 1, sand filtration cylinder 2, Protein Skimmer 3, biological drip box 4, biological treating tank 5, adjust cylinder 6,
Sterilizing unit 7, air conditioner 8, sewage treatment cylinder 10, distribution reservoir 11；
The culturing jar 1, is equipped with top inlet pipeline 12 and bottom outlet pipeline 13, and the bottom outlet pipeline 13 is logical
Cross three-way connection connection water return pipeline 14 and discharge pipe line 15；
The sand filtration cylinder 2 is located at 1 downstream of culturing jar, is connected to water return pipeline 14, and the water inlet position of husky filtering jar 2 connects water
A is pumped, cultivation water is made to enter sand filtration cylinder 2 by culturing jar 1；
The Protein Skimmer 3 is located at 2 downstream of husky filtering jar, if there are two water outlet, a water outlet connection biology drop
Box 4 is flowed, another water outlet connects sterilizing unit 7；
The biology drip box 4 accepts the cultivation water handled through Protein Skimmer 3, and filter cotton and life are housed in drip box
Object carrier filler, drip cassette bottom portion are evenly arranged with hole；
The biological treating tank 5 accepts the cultivation water handled through biological drip box 4, and side wall has hole；
The inlet pipeline for adjusting cylinder 6 is connected with distribution reservoir 11, and outlet pipeline is connected with sterilizing unit 7, inlet pipeline
The water of distribution reservoir 11 is introduced equipped with water pump C, outlet pipeline is equipped with water pump B so that water enters sterilizing unit 7；Adjusting cylinder 6 can
Carry out biological treatment and balance system flow；
The sterilizing unit 7 is used to handle Protein Skimmer 3 or adjusts the water that cylinder 6 is discharged, and outlet pipeline connection is cold
Warming-up 8；
The air conditioner 8 is used to adjust the water temperature of the cultivation water of the outflow of sterilizing unit 7；The water that air conditioner 8 flows out is via top
It holds inlet pipeline 12 to enter culturing jar 1, is recycled；
The sand filtration cylinder 2 and adjusting cylinder 6 is equipped with water outlet and is connected to discharge pipe line 15, discharge pipe line 15 and sewage treatment
Cylinder 10 is connected to.
In a preferred embodiment, in the circulating water cultivation device, between air conditioner 8 and top inlet pipeline 12
On pipeline, it is also equipped with Physico-chemical tests instrument 9；Preferably, in the Physico-chemical tests instrument 9, comprising: temperature control device, temperature measuring equipment are molten
Oxygen measurement device, pH measurement device, conductivity measuring device；Preferably, the temperature control device connection alarm system and changes in temperature
Machine switch, when temperature of cultivation is more than setting temperature-control range, system alarm, air conditioner are closed.
In another preferred example, there are 1~50 culturing jars 1 for the device；Preferably, the bottom of the culturing jar 1
Portion forms the paddy structure that surrounding is high, centre is low, and paddy lowest part connects bottom outlet pipeline 13；Preferably, outlet pipeline 13 and support
Grow the starting point installation excrement collector of the connection of cylinder 1.
In another preferred example, in the circulating water cultivation device, the culturing jar 1 adjusts cylinder 6,11 and of distribution reservoir
The capacity of sewage treatment cylinder 10 according to volume ratio be 6:2:3:12.
In another preferred example, in the circulating water cultivation device, the top inlet pipeline 12 is that direction is adjustable
Nodal pattern；Preferably, the top inlet pipeline of the culturing jar 1 is installed on apart from 5 ± 2cm of cylinder body top；Preferably at 5 ± 1cm.
In another preferred example, in the circulating water cultivation device, in the biology drip box 4, it is equipped with water sampling
Device detects content of microorganisms in water body with random acquisition water sample；Preferably, at the bottom of the biological drip box 4 and biology
The side wall of reason slot 5 is evenly distributed with diameter or side length is 1 ± 0.3cm；The more preferably hole of 1 ± 0.2cm.
In another preferred example, in the circulating water cultivation device, the Protein Skimmer 3 is equipped with flow adjusting and opens
It closes, so that water circulation reaches balance；Or
The adjusting cylinder 6 is half open device, and open section accepts the cultivation water handled through biological drip box 4；Or
The sewage treatment cylinder 10 is equipped with chemical agent chlorination equipment, to carry out disinfection processing to cultivation water；Or
Oxygen-increasing device is equipped in the circulation line of the device；Or
Pneumatic type filter is equipped in the circulation line of the device；Or
In the distribution reservoir 11, it is equipped with electric mixer；Or
The biological treating tank 5 has bottom wall common on one side with cylinder 6 is adjusted；Or
Between the Protein Skimmer 3 and sterilizing unit 7, equipped with switch to control the connection between them and not connect
It is logical；Or
Between the Protein Skimmer 3 and biological drip box 4, equipped with switch with control the connection between them with not
Connection；Or
The sterilizing unit 7 is ultraviolet sterilization device.
In another preferred example, the culturing jar 1, sand filtration cylinder 2, Protein Skimmer 3, biological drip box 4, at biology
It manages slot 5, adjust between cylinder 6, sterilizing unit 7, air conditioner 8, sewage treatment cylinder 10 or each device of distribution reservoir 11, control can be set
Connection or disconnected switch between neighboring devices.
In another aspect of this invention, the purposes of the circulating water cultivation device is provided, for aquaculture of aquatic animal and
Medication assessment；Preferably, the medication assessment includes: to evaluate the effect of aquatic livestock vaccine；More preferably, the aquatic products
Animal includes: fish, shrimp, crab, soft-shelled turtle, tortoise, shellfish or the frog.
In another aspect of this invention, a kind of aquatic livestock circulating water cultivation method is provided, the method includes: by water
It produces animal to be placed in the culturing jar 1 of the device, closes discharge pipe line 15 and water pump C, fill Protein Skimmer 3 and sterilization
7 are set not to be connected to；Water return pipeline 14, water pump A and water pump B are opened, top inlet pipeline 12, Protein Skimmer 3, sterilization dress are opened
It sets 7, air conditioner, 8 cultivation water and enters sand filtration cylinder 2 through water pump A, Protein Skimmer 3 is entered after classified filtering precipitates, through albumen
Enter biological drip box 4 after separation, flows into biological treating tank 5 later, enter after microbiological treatment and adjust cylinder 6, water pump B will be adjusted
Recirculated water is delivered to sterilizing unit 7 in section cylinder, enters air conditioner 8 through sterilization processing, it is temperature regulated after by top inlet pipeline
12 enter culturing jar 1, carry out cultivation water recycling.
In a preferred embodiment, the aquatic livestock is the aquatic livestock for needing to carry out medication (such as vaccine) assessment.
In another preferred example, in the breeding process, further include drug is given to aquatic livestock, and to the effect of drug into
Row evaluation.
In another preferred example, the drug is vaccine.
In another preferred example, vaccine potency evaluation is carried out to aquatic livestock.Preferably, including: first to aquatic livestock
Vaccine immunity is carried out, carries out attacking poison with strain (such as pathogenic strain or Causative virus strain) later, is attacked against each other to aquatic livestock malicious resistance to
It is measured by ability, evaluates vaccine potency.
In another preferred example, after cultivation, close discharge pipe line 15, Protein Skimmer 3, biological drip box 4,
Water pump C is connected to Protein Skimmer 3 not with biological drip box 4；Open water return pipeline 14, water pump A, water pump B, protein point
From device 3, sterilizing unit 7, air conditioner 8, top inlet pipeline 12；Disinfectant is added in cultivation water, cultivation water enters through water pump A
Sand filtration cylinder enters Protein Skimmer 3 after classified filtering precipitates, and sterilizing unit 7 is entered after Protein Separation, through sterilization processing
Enter culturing jar 1 by by air conditioner 8 and top inlet pipeline 12, carries out cultivation water disinfection treatment；Preferably, being opened after disinfection
Discharge pipe line 15 is opened, sterilized cultivation water enters sewage treatment cylinder 10 by discharge pipe line 15, carries out secondary sterilization, discharge.
Other aspects of the invention are apparent to those skilled in the art due to this disclosure
's.
Detailed description of the invention
Fig. 1, the present invention evaluate the structural schematic diagram of the circulating water culture system of fish vaccine effect.Wherein, each attached drawing mark
Note is described as follows:
1. culturing jar, 2. sand filtration cylinders,
3. Protein Skimmer, 4. biological drip boxes,
5. biological treating tank, 6. adjust cylinder,
7. ultraviolet sterilization device, 8. air conditioners,
9. Physico-chemical tests instrument, 10. sewage treatment cylinders,
11. distribution reservoir, 12. top inlet pipelines,
13. bottom outlet pipeline, 14. water return pipelines,
15. discharge pipe line；
A, B, C are water pump.
In figure, solid arrow direction indicates that recirculating aquaculture water flow direction, dotted arrow direction indicate cultivation water discharge side
To.
Specific embodiment
The present inventor passes through in-depth study, provides a kind of recirculated water that can be used for aquaculture of aquatic animal and medication assessment
Cultivation apparatus.Circulating water cultivation device of the invention combines aquaculture of aquatic animal, water process, water quality monitoring, it can be achieved that fresh water
Or the cultivation of recirculated water or hydrostatic, cultivation water process and discharge, the detection of cultivation water physical and chemical parameter of seawater class aquatic livestock, guarantee to use
Medicine assesses (such as vaccine potency evaluation), and breeding environment is excellent in the process, experiment condition is controllable.
As used herein, " aquatic livestock " refers to the animal survived in water (including fresh water or seawater)；It is more special
Do not refer to wild or cultivation the animal survived in water and with economic value.For example, " aquatic livestock " includes
But it is not limited to: fish, shrimp, crab, soft-shelled turtle, tortoise, shellfish, frog etc..
As used herein, " recirculated water " includes seawater, fresh water；The seawater can be the seawater of nature acquisition
Or artificial seawater (seawater manually prepared)；The fresh water can be the fresh water or artificial light of nature acquisition
Water (such as distilled water, mineral water).
Circulating water cultivation device
Circulating water cultivation device of the invention, comprising: culturing jar, sand filtration cylinder, Protein Skimmer, biological drip box, life
Object treatment trough adjusts cylinder, sterilizing unit, air conditioner, sewage treatment cylinder and distribution reservoir.These apparatus systems are connected, so as to side
Just, it is efficiently applied to aquaculture of aquatic animal and medication assessment.
The culturing jar be applied to accommodate aquatic livestock device, for aquatic livestock provide suitable for survive and breed
Place.It may include at least one culturing jar in circulating water cultivation device of the invention.It preferably include more than one cultivation
Cylinder；For example including 1~100 culturing jar, it can be 80,70,60,50,40,30,20,10,5.
The size and shape of the culturing jar is not particularly limited, can be according to the big of the aquatic livestock cultivated
Small, type determines.Preferably, the body shape of the culturing jar is cylinder, sphere or spheroid.
As preferred embodiment of the invention, the bottom of the culturing jar is designed as the paddy structure that surrounding is high, centre is low, paddy
Lowest part connects bottom outlet pipeline, and the starting end position that bottom outlet pipeline is connect with culturing jar installs excrement collector, with
Retention excrement, reduction excrement enter in outlet pipeline, uniformly distributed hole on excrement collector, can be according to the excrement of the aquatic livestock of cultivation
Just size selects pore diameter appropriate.Excrement be enriched with to a certain degree when, the excrement collector is set as can quilt
It removes and clears up.
Top inlet pipeline is arranged in the top of the culturing jar；Bottom outlet pipe is arranged in the lower part of the culturing jar
Road；The bottom outlet pipeline connects three-way connection, is separately connected water return pipeline and discharge pipe line.
As preferred embodiment of the invention, the top inlet pipeline of the culturing jar be installed on apart from cylinder body top 5 ±
2cm, preferably at 5 ± 1cm, water inlet is set as direction adjustable type, when use adjustable water discharging direction, avoid water circulation
In the presence of " dead angle ".
The water of culturing jar can enter husky filtering jar via water return pipeline.Sand (middle sand, partial size are filled in the sand filtration cylinder
0.35~0.5mm).The sand filtration cylinder is connected to water return pipeline, and the water inlet position of sand filtration cylinder connects water pump A, and cultivation water can be by
Culturing jar enters sand filtration cylinder, after removing larger particles via husky filtering jar classified filtering precipitating, is flowed out by the water outlet of husky filtering jar.Water
Pump A can enter sand filtration cylinder by fish jar for cultivation water and provide power.
In the downstream of husky filtering jar, it is connected with Protein Skimmer, applied to the protein ingredient for separating and going in water removal.It is described
Protein Skimmer be equipped at least one water inlet and at least two water outlets, water inlet accepts the cultivation of husky filtering jar outflow
Water, at least one water outlet connect biological drip box, at least one water outlet connects sterilizing unit, it can be achieved that cultivation cyclic system
The sterilizing operation of system or the cultivating system in addition to biological treatment system.
As preferred embodiment of the invention, the Protein Skimmer is additionally provided with flow control valve, so that water circulation
Reach balance.
The biology drip box (abbreviation drip box) accepts the cultivation water handled through Protein Skimmer, for water body of degrading
The harmful substances such as middle ammonia nitrogen, nitrite.Biochemical filtration cotton and bio-carrier are filled in the drip box, and (culture has
Nitrobacteria, bacillus etc., the harmful substances such as degradable water body ammonia nitrogen, nitrite)；Preferably, bio-carrier is
The active microorganism of still degradable water body ammonia nitrogen when water temperature is lower.Drip cassette bottom portion is evenly distributed with hole so that being processed
Cultivation water enter in biological treating tank.It is 1 ± 0.2cm, the more preferably hole of 1 ± 0.1cm that the hole, which can be diameter,.
As preferred embodiment of the invention, the drip box is equipped with water sampler, with random acquisition water sample, detects water body
Middle content of microorganisms avoids experimental error caused by spot sampling.
The biological treating tank accepts the cultivation water handled through biological drip box, is the sink with opening, preferably
It is open or half open sink, and it is 1 ± 0.3cm, the more preferably hole of 1 ± 0.2cm that basin sidewall, which is evenly distributed with diameter,.Preferably
The bottom wall that ground, the bottom of the biological treating tank and the adjusting cylinder have one side common.Culture has nitrification in biological treating tank
Bacterium, bacillus etc., the harmful substances such as degradable water body ammonia nitrogen, nitrite.
The adjusting cylinder is able to carry out biological treatment and balance system flow, is equipped with water inlet pipe and water outlet pipe, into
Water lines are connected with distribution reservoir, and outlet pipeline is connected with sterilizing unit, and inlet pipeline is equipped with water pump C to be introduced into distribution reservoir
Water, outlet pipeline are equipped with water pump B so that water enters sterilizing unit.
The distribution reservoir is used to supplement fresh water for culture circulation system.It is described as preferred embodiment of the invention
In distribution reservoir, it is equipped with electric mixer, when using formulated water (such as artificial seawater), is prepared for automatic stirring, stirring
Purpose be so that solid solute (such as sea salt) can sufficiently dissolve.
As preferred embodiment of the invention, the biological treating tank is comprised in the adjusting cylinder；Correspondingly, described
Adjusting cylinder is half open device, and open section is located at the top of biological treating tank opening, in favor of accepting biological drip box outflow
Cultivation water, it can be achieved that two step biological treatment process to cultivation water.
The sterilizing unit is used to handle Protein Skimmer or adjusts the water of cylinder discharge, is provided at least two water inlets
Pipeline and at least one outlet pipeline.At least one inlet pipeline is connected through Protein Skimmer, can handle Protein Skimmer
The cultivation water of outflow；The connection of at least one inlet pipeline adjusts cylinder, and processing adjusts the water of cylinder discharge.
The sterilizing unit can be a variety of devices for microorganism, particularly bacterium with killing effect；More
Preferably, the sterilizing unit is ultraviolet sterilization device.
The air conditioner is located at the downstream of sterilizing unit, can adjust the temperature for flowing through its cultivation water.Can according to institute
The difference of the aquatic livestock of cultivation adjusts the temperature of cultivation water, and suitable breeding environment is provided for aquatic livestock.
As preferred embodiment of the invention, on the pipeline between air conditioner and top inlet pipeline, it is also equipped with physics and chemistry
Detector.Preferably, in the Physico-chemical tests instrument, comprising: temperature control device, temperature measuring equipment, dissolved oxygen measurement device, pH measurement dress
It sets, conductivity measuring device；Preferably, the temperature control device connection alarm system and air conditioner switch, when temperature of cultivation is super
When crossing the temperature-control range of setting, system alarm, air conditioner are closed.
The sewage treatment cylinder accepts the water of discharge pipe line discharge, is equipped with water inlet pipe and water outlet pipe, it can be achieved that right
The sterilizing operation of cultivation water.As preferred embodiment of the invention, it is additionally provided with chlorination equipment, to carry out disinfection place to cultivation water
Reason；Preferably, the chlorination equipment is equipped with the chemical agent with antimicrobial activity.The chlorination equipment can to the water flowed through into
Row is further processed, and guarantees the safety of the water of the device of the invention discharge.
In circulating water cultivation device of the invention, the sand filtration cylinder and adjusting cylinder are equipped with water outlet and discharge pipe line company
Logical, discharge pipe line is connected to sewage treatment cylinder, can be discharged and is not required to recycled water.
As preferred embodiment of the invention, the culturing jar adjusts the capacity of cylinder, distribution reservoir, sewage treatment cylinder according to one
Fixed ratio setting is suitable for the cycle applications of recirculated water.Preferably, the volume ratio are as follows: 6:2:3:12.Certainly, in the volume
Than on the basis of, the capacity of each device suitably can be floated or be floated downward, such as float according to volume or float downward 20%,
15%, 10% or 5%.
As preferred embodiment of the invention, the circulator is equipped with independent oxygen-increasing device.Oxygen-increasing device can basis
It needs according on the position appropriate of entire circulation line.
As preferred embodiment of the invention, for being metabolized vigorous aquatic livestock, in the pipeline of the device of the invention, may be used also
Pneumatic type filter is added, first increases excrement, residual bait collecting amount in culturing jar, Simple filter second can be added in filter
Cotton, biologic packing material etc., to reduce water body harmful substance contents.
The application of circulating water cultivation device
Circulating water cultivation device of the invention can be applied to Aquatic farming animals, aquaculture of aquatic animal and medication assessment；Compared with
Goodly, medication assessment includes: to evaluate the effect of aquatic livestock vaccine；More preferably, the aquatic livestock includes:
Fish, shrimp, crab, soft-shelled turtle, tortoise, shellfish, frog etc..
In Aquatic farming animals or medication evaluation process, aquatic livestock is placed in the culturing jar of the device of the invention first
In, discharge pipe line and water pump are closed, is connected to Protein Skimmer not with sterilizing unit；Open water return pipeline, water pump A and water pump
B opens top inlet pipeline, Protein Skimmer, sterilizing unit, air conditioner, distribution reservoir；Cultivation water enters sand filtration through water pump A
Cylinder enters Protein Skimmer after classified filtering precipitates, and biological drip box is entered after Protein Separation, is flowed at biology later
Slot is managed, enters after microbiological treatment and adjusts cylinder, water pump B will adjust recirculated water in cylinder and be delivered to sterilizing unit, through sterilization processing
Into air conditioner, culturing jar entered by top inlet pipeline after temperature regulated, carries out cultivation water recycling.
As preferred embodiment of the invention, the aquatic livestock is to need to carry out medication assessment, such as needs to carry out vaccine
The aquatic livestock of capability assessment.It in the breeding process, further include that drug (such as vaccine) is given to aquatic livestock, and to the effect of drug
Fruit is evaluated.
It when carrying out vaccine potency evaluation to aquatic livestock, generally comprises: vaccine immunity being carried out to aquatic livestock first and (is connect
Kind), it through a period of time after being immunized, carries out attacking poison with strain (such as pathogenic strain or Causative virus strain), attack against each other poison to aquatic livestock
Tolerance be measured, evaluate vaccine potency.For example, evaluating vaccine effect by experimental animal death condition.
In cultivation and medication evaluation process, cultivation water removes larger of excrement, residual bait in water body etc. by sand filtration cylinder
Grain object, through the molecule in Protein Skimmer removal water body, through ammonia nitrogen, nitrite in biological drip box degradation water body etc.
Harmful substance, the harmful substance in water body of further degrading through biological treating tank, kills microorganisms in water through ultraviolet sterilization device,
Enter culturing jar after air conditioner adjusts cultivation water temperature, constitutes recirculation system.
After Aquatic farming animals or medication are assessed, close discharge pipe line, Protein Skimmer, biological drip box,
Water pump C is connected to Protein Skimmer not with biological drip box；Open water return pipeline, water pump A, water pump B, Protein Skimmer,
Sterilizing unit, air conditioner, top inlet pipeline；Disinfectant is added in cultivation water, cultivation water enters sand filtration cylinder through water pump A, warp
Enter Protein Skimmer after classified filtering precipitating, sterilizing unit is entered after Protein Separation, through by changes in temperature after sterilization processing
Machine and top inlet pipeline enter culturing jar, carry out cultivation water disinfection treatment；Preferably, discharge pipe line is opened, through disappearing after disinfection
The cultivation water of poison enters sewage treatment cylinder by discharge pipe line, carries out secondary sterilization, discharge.Disinfectant can be added to cultivation water and follow
Multiple positions of loop system, as long as enable to disinfectant to come into full contact with cultivation water, such as be added to adjusting cylinder, culturing jar etc.
It is interior.
Above-mentioned sterilization method can thoroughly carry out disinfection, and avoid being discharged harmful water (such as because carrying out medication assessment
Lead to the water of pollution)；And it is possible to which thimerosal (such as new gill goes out) is avoided to destroy established biological treatment system.
The device of the invention has the beneficial effect that:
The present invention provides a kind of circulating water cultivation devices that can be used for carrying out aquatic livestock medication assessment.Of the invention
Circulating water cultivation device includes cultivation water compounding system, cultivating system, water treatment system and water quality monitoring system.Using the present invention
Device, can the cultivation of real aquatic livestock, recirculated water or hydrostatic cultivation, cultivation water process and discharge, the inspection of cultivation water physical and chemical parameter
It surveys, guaranteeing medication assessment (such as vaccine potency evaluation), breeding environment is excellent in the process, experiment condition is controllable.
The device of the invention can be used for evaluating the effect of the candidate vaccine of the aquatic livestock in seawater or fresh water.Currently, extra large
Water cultivation fingerling such as turbot, lefteye flounder, grouper and freshwater aquiculture fingerling such as grass carp, carp, Tilapia mossambica has disease to break out
Relevant report.The present invention can adjust cultivation temperature, salinity according to cultivation fingerling, meet different fingerling candidate vaccine exploitation works
Make.
The device of the invention, which can carry out disinfection to recirculated water, to be handled and does not destroy biological water treatment system.At biology
Reason can effectively remove the harmful substances such as water body ammonia nitrogen, nitrite.The present invention can be achieved to following in addition to biological treatment system
Ring water carries out disinfection processing, reduces the settling time of biological treatment system in different batches experiment, provides cultivating system
Utilization efficiency.
The device of the invention is provided with sewage treatment cylinder, can avoid experimental water and discharges to other water body environments, it is ensured that comments
Valence tests the safety to ambient enviroment.
The device of the invention additionally provides water quality detection system, it can be ensured that vaccine evaluation tests the ring controllable in physico chemical factor
It is carried out under border, avoids the experimental error as caused by external environment.
Circulating water cultivation device of the invention can be ground for drug (such as vaccine) R&D work of aquatic livestock by laboratory
Study carefully the development to clinical research, lays the foundation for the application study and industrialized development of the drug (such as vaccine) of aquatic livestock.
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In the following examples, the experimental methods for specific conditions are not specified, usually according to conventional strip
Part, or according to the normal condition proposed by manufacturer.Unless otherwise defined, all professional and scientific terms as used herein and sheet
Meaning known to the skilled person of field is identical.In addition, any method similar to or equal to what is recorded and material all may be used
Applied in the present invention.The preferred methods and materials described herein are for illustrative purposes only.
Embodiment 1, a kind of circulating water cultivation device for evaluating fish vaccine effect
In the present embodiment, a kind of circulating water cultivation device for evaluating fish vaccine effect is provided, as shown in Figure 1 comprising
Following device: culturing jar 1, Protein Skimmer 3, biological drip box 4, biological treating tank 5, adjusts cylinder 6, ultraviolet kills sand filtration cylinder 2
Bacterium device 7, air conditioner 8, Physico-chemical tests instrument 9, sewage treatment cylinder 10, distribution reservoir 11.Also, the circulator is additionally provided with independent
Oxygen-increasing device is accessed in culturing jar positioned at the top of entire circulator by tracheae.
Wherein, culturing jar 1 is arranged 6, and shape is cylindrical, and the volume of each culturing jar 1 is about 220,000cm3
(220L).It is provided with top inlet pipeline 12 above each culturing jar, is provided with bottom below each culturing jar
Portion's outlet pipeline 13 is connected to water return pipeline 14 and discharge pipe line 15 by three-way connection.
The sand filtration cylinder 2 is located at 1 downstream of culturing jar, is connected to water return pipeline 14, and the water inlet position of husky filtering jar 2 connects water
A is pumped, cultivation water is made to enter sand filtration cylinder 2 by culturing jar 1.10-15 ton/hours of 2 rated flow of sand filtration cylinder, filter area 1.5m2, directly
Diameter 700mm, the sand for being 0.35~0.5mm built with average grain diameter precipitate in the cultivation water flowed through so as to classified filtering
Larger particles.Husky filtering jar further includes that a water outlet is connected to discharge pipe line 15.
The Protein Skimmer 3 is located at 3 downstream of protein separator, if there are two water outlet, a water outlet connection life
Object drip box 4, another water outlet connect sterilizing unit 7.Protein Skimmer 3 be pressure type protein separator, rated flow:
5-15 ton/hours, reaction vessel size: φ 200*H1200mm, mean air entry amount: > 1000ml/m, reaction time: > 2m.
The biology drip box 4 accepts the cultivation water handled through Protein Skimmer 3, and biochemical filtration cotton is housed in drip box
And (wherein culture has nitrobacteria, bacillus etc., the nuisances such as degradable water body ammonia nitrogen, nitrite to bio-carrier
Matter), drip cassette bottom portion is evenly arranged with hole so that water flow passes through, and pore diameter is about 1cm.The specification of biological drip box 4
About 30 × 40 × 50cm.
Biological treating tank 5 accepts the cultivation water handled through biological drip box 4, is located at and adjusts within cylinder 6, and the two has
Common bottom surface, biological treating tank 5 are half uncovered structure, and side wall is evenly distributed with pore structure, and hole is circle, and diameter is
1cm.The volume of biological treating tank 5 is about 100L, and interior culture has nitrobacteria, bacillus etc., degradable water body ammonia nitrogen, Asia
The harmful substances such as nitrate.
It adjusts cylinder 6 and is equipped with water inlet pipe and water outlet pipe, inlet pipeline is connected with distribution reservoir 11, and outlet pipeline and sterilization fill
It sets 7 to be connected, inlet pipeline is equipped with water pump C to introduce the water of distribution reservoir 11, and outlet pipeline is equipped with water pump B so that water enters sterilization dress
Set 7；It adjusts cylinder 6 and is able to carry out biological treatment and balance system flow.The volume for adjusting cylinder 6 is about 200L.
Fresh water is housed, for supplementing fresh water for culture circulation system, wherein being additionally provided with electronic in distribution reservoir 11
Blender.
Sterilizing unit 7 is a kind of ultraviolet sterilization device, for handling Protein Skimmer 3 or adjusting the water of the discharge of cylinder 6.It should
The technical parameter of ultraviolet sterilization device are as follows: power 120W, fluorescent tube 3, casing is quartz material, is electronically started, using purple
The 253.7nm light wave that outside line C-band issues irradiates the instantaneous time of bacterial viruses various in water.
The air conditioner 8 is used to adjust the water temperature of the cultivation water of the outflow of sterilizing unit 7, technical parameter are as follows: power 2HP,
Refrigerating capacity 5800W, heating capacity 6200W, quantity of circulating water 6m3/ H, 63 × 54 × 50cm of fuselage size.In air conditioner be equipped with heating and
Refrigeration equipment is to adjust water temperature.
The water that air conditioner 8 flows out flows into physical and chemical detector 9.It include: temperature control device, thermometric in the Physico-chemical tests instrument 9
Device, dissolved oxygen measurement device, pH measurement device, conductivity measuring device.The temperature control device connection alarm system and changes in temperature
Machine switch, when temperature of cultivation is more than setting temperature-control range, system alarm, air conditioner are closed.
The water that Physico-chemical tests instrument 9 flows out enters culturing jar 1 by top inlet pipeline 12, is recycled.
Sand filtration cylinder 2 and adjusting cylinder 6 are equipped with water outlet and are connected to discharge pipe line 15, and discharge pipe line 15 and sewage treatment cylinder 10 connect
It is logical.The volume 2000L of sewage treatment cylinder 10.
Embodiment 2, the circulating water cultivation device evaluation fish vaccine effect that should have embodiment 1
Using turbot as tested fish in the present embodiment.Isolated from Diseased Scophthalmus maximus is made and evaluates the method packet of fish vaccine effect
It includes: turbot is placed in the culturing jar 1 of device described in embodiment 1, close discharge pipe line 15 and water pump C, make protein point
It is not connected to from device 3 with sterilizing unit 7；Water return pipeline 14, water pump A and water pump B are opened, top inlet pipeline 12, protein are opened
Separator 3, sterilizing unit 7, air conditioner 8；Cultivation water enters sand filtration cylinder 2 through water pump A, and protein is entered after classified filtering precipitates
Separator 3 enters biological drip box 4 after Protein Separation, flows into biological treating tank 5 later, enter after microbiological treatment and adjust
Cylinder 6 is saved, water pump B will adjust recirculated water in cylinder and be delivered to sterilizing unit 7, enter air conditioner 8 through sterilization processing, after temperature regulated
Culturing jar 1 is entered by top inlet pipeline 12, carries out cultivation water recycling.
Vaccination of fish Edwardsiella tarda live vaccine is placed in the device of embodiment 1 and cultivates.After vaccine immunity 4 weeks,
Which kind of thrown down the gauntlet with Edwardsiella tarda strain (specific EIB202), the death condition by observing fish evaluates vaccine effect
Fruit.
After cultivation and vaccine potency evaluation experimental, thorough disinfection processing need to be carried out to cultivation recirculated water.Adjusting cylinder
Middle addition disinfectant closes discharge pipe line 15, Protein Skimmer 3, biological drip box 4, water pump C, make Protein Skimmer 3 with
Biological drip box 4 is not connected to；Open water return pipeline 14, water pump A, water pump B, Protein Skimmer 3, sterilizing unit 7, air conditioner 8,
Top inlet pipeline 12；Cultivation water enters sand filtration cylinder through water pump A, Protein Skimmer 3 is entered after classified filtering precipitates, through egg
Enter sterilizing unit 7 after white separation, through entering culturing jar 1 by air conditioner 8 and top inlet pipeline 12 after sterilization processing, carries out
Cultivation water disinfection treatment.
After disinfection, discharge pipe line 15 is opened, sterilized cultivation water flows into discharge pipe line 15 from husky filtering jar, adjusting cylinder, by
Discharge pipe line 15 enters sewage treatment cylinder 10, after carrying out secondary sterilization, discharge.
It should be understood that after reading the above teachings of the present invention, those skilled in the art can make the present invention
Various changes or modification, these equivalent forms also fall within the scope of the appended claims of the present application.
Claims (17)
1. a kind of circulating water cultivation device assessed for aquaculture of aquatic animal and medication, which is characterized in that the device includes: feeding
It grows cylinder (1), sand filtration cylinder (2), Protein Skimmer (3), biological drip box (4), biological treating tank (5), adjust cylinder (6), sterilization
Device (7), air conditioner (8), sewage treatment cylinder (10), distribution reservoir (11)；
The culturing jar (1) is 5~50, is equipped with top inlet pipeline (12) and bottom outlet pipeline (13), the bottom
Outlet pipeline (13) passes through three-way connection connection water return pipeline (14) and discharge pipe line (15)；
The sand filtration cylinder (2) is located at culturing jar (1) downstream, is connected to water return pipeline (14), and the water inlet position of husky filtering jar (2) connects
Water pump A makes cultivation water enter sand filtration cylinder (2) by culturing jar (1)；
The Protein Skimmer (3) is located at husky filtering jar (2) downstream, if there are two water outlet, a water outlet connection biology drop
It flows box (4), another water outlet connects sterilizing unit (7)；The bottom of the biological drip box (4) is evenly distributed with diameter or side length is 1
The hole of ± 0.3cm；
The biology drip box (4) accepts the cultivation water handled through Protein Skimmer (3), and filter cotton and life are housed in drip box
Object carrier filler, drip cassette bottom portion are evenly arranged with hole；
The biological treating tank (5) accepts the cultivation water handled through biological drip box (4), and side wall is evenly distributed with diameter or side length is 1
The hole of ± 0.3cm；
The inlet pipeline for adjusting cylinder (6) is connected with distribution reservoir (11), and outlet pipeline is connected with sterilizing unit (7), water inlet pipe
Road is equipped with water pump C to introduce the water of distribution reservoir (11), and outlet pipeline is equipped with water pump B so that water enters sterilizing unit (7)；Adjust cylinder
(6) biological treatment and balance system flow are able to carry out；Bottom wall that biological treating tank (5) and adjusting cylinder (6) have one side common,
Biological treating tank (5), which is comprised in, to be adjusted in cylinder (6)；The biological treating tank (5) is open or half open sink；The tune
Saving cylinder (6) is half open device, and open section is located at the top of biological treating tank (5) opening, is accepted at through biological drip box (4)
The cultivation water of reason；
The sterilizing unit (7) is used to handle Protein Skimmer (3) or adjust the water of cylinder (6) discharge, outlet pipeline connection
Air conditioner (8)；
The air conditioner (8) is used to adjust the water temperature of the cultivation water of sterilizing unit (7) outflow；Air conditioner (8) outflow water via
Top inlet pipeline (12) enters culturing jar (1), is recycled；
The sand filtration cylinder (2) and adjusting cylinder (6) is equipped with water outlet and is connected to discharge pipe line (15), discharge pipe line (15) and sewage
Handle cylinder (10) connection.
2. circulating water cultivation device as described in claim 1, which is characterized in that in air conditioner (8) and top inlet pipeline
(12) on the pipeline between, Physico-chemical tests instrument (9) are also equipped with.
3. circulating water cultivation device as claimed in claim 2, which is characterized in that in the Physico-chemical tests instrument (9), comprising: temperature
Control device, temperature measuring equipment, dissolved oxygen measurement device, pH measurement device, conductivity measuring device.
4. circulating water cultivation device as claimed in claim 3, which is characterized in that the described temperature control device connection alarm system and
Air conditioner switch, when temperature of cultivation is more than setting temperature-control range, system alarm, air conditioner are closed.
5. circulating water cultivation device as described in claim 1, which is characterized in that the bottom of the culturing jar (1) forms four
Zhou Gao, intermediate low paddy structure, paddy lowest part connect bottom outlet pipeline (13).
6. circulating water cultivation device as claimed in claim 5, which is characterized in that outlet pipeline (13) is connect with culturing jar (1)
Starting point install excrement collector.
7. circulating water cultivation device as described in claim 1, which is characterized in that the culturing jar (1) adjusts cylinder (6), water distribution
The capacity of pond (11) and sewage treatment cylinder (10) according to volume ratio be 6:2:3:12.
8. circulating water cultivation device as described in claim 1, which is characterized in that the top inlet pipeline (12) is direction
Adjustable type.
9. circulating water cultivation device as claimed in claim 8, which is characterized in that the top inlet pipeline of the culturing jar (1)
It is installed at 5 ± 2cm of cylinder body top.
10. circulating water cultivation device as described in claim 1, which is characterized in that in the biology drip box (4), be equipped with water
Sample collector detects content of microorganisms in water body with random acquisition water sample.
11. circulating water cultivation device as described in claim 1, which is characterized in that the Protein Skimmer (3) is equipped with flow
Regulating switch, so that water circulation reaches balance；Or
The sewage treatment cylinder (10) is equipped with chemical agent chlorination equipment, to carry out disinfection processing to cultivation water；Or
Oxygen-increasing device is equipped in the circulation line of the circulating water cultivation device；Or
Pneumatic type filter is equipped in the circulation line of the circulating water cultivation device；Or
In the distribution reservoir (11), it is equipped with electric mixer；Or
Between the Protein Skimmer (3) and sterilizing unit (7), equipped with switch to control the connection between them and not connect
It is logical；Or
Between the Protein Skimmer (3) and biological drip box (4), equipped with switch with control the connection between them with not
Connection；Or
The sterilizing unit (7) is ultraviolet sterilization device.
12. the purposes of any circulating water cultivation device of claim 1-11, which is characterized in that be used for aquaculture of aquatic animal
And medication assessment.
13. purposes as claimed in claim 12, which is characterized in that the medication assessment includes: evaluation aquatic livestock epidemic disease
The effect of seedling.
14. purposes as claimed in claim 13, which is characterized in that the aquatic livestock includes: fish, shrimp, crab, soft-shelled turtle, tortoise, shellfish
Or the frog.
15. a kind of aquatic livestock circulating water cultivation method, which is characterized in that the method includes: that aquatic livestock is placed in power
Benefit require 1 described in circulating water cultivation device culturing jar (1) in, close discharge pipe line (15) and water pump C, make Separation of Proteins
Device (3) is not connected to sterilizing unit (7)；Unlatching water return pipeline (14), water pump A and water pump B, unlatching top inlet pipeline (12),
Protein Skimmer (3), sterilizing unit (7), air conditioner (8) cultivation water enter sand filtration cylinder (2) through water pump A, heavy through classified filtering
Enter Protein Skimmer (3) behind shallow lake, biological drip box (4) entered after Protein Separation, flows into biological treating tank (5) later,
Enter after microbiological treatment and adjust cylinder (6), water pump B will adjust recirculated water in cylinder and be delivered to sterilizing unit (7), through sterilization processing
Into air conditioner (8), it is temperature regulated after by top inlet pipeline (12) enter culturing jar (1), carry out cultivation water recycling.
16. method as claimed in claim 15, which is characterized in that after cultivation, close discharge pipe line (15), protein
Separator (3), biological drip box (4), water pump C, are connected to Protein Skimmer (3) not with biological drip box (4)；Open return water
Pipeline (14), water pump A, water pump B, Protein Skimmer (3), sterilizing unit (7), air conditioner (8), top inlet pipeline (12)；
Disinfectant is added in cultivation water, cultivation water enters sand filtration cylinder through water pump A, and Protein Skimmer is entered after classified filtering precipitates
(3), enter sterilizing unit (7) after Protein Separation, after sterilization processing through by air conditioner (8) and top inlet pipeline (12) into
Enter culturing jar (1), carries out cultivation water disinfection treatment.
17. the method described in claim 16, which is characterized in that after disinfection, open discharge pipe line (15), sterilized cultivation
Water enters sewage treatment cylinder (10) by discharge pipe line (15), carries out secondary sterilization, discharge.
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