CN105543070A - Culture bottle body and making method thereof - Google Patents
Culture bottle body and making method thereof Download PDFInfo
- Publication number
- CN105543070A CN105543070A CN201610090972.3A CN201610090972A CN105543070A CN 105543070 A CN105543070 A CN 105543070A CN 201610090972 A CN201610090972 A CN 201610090972A CN 105543070 A CN105543070 A CN 105543070A
- Authority
- CN
- China
- Prior art keywords
- bottle
- plastic
- culturing
- bottle body
- culturing bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29D—PRODUCING PARTICULAR ARTICLES FROM PLASTICS OR FROM SUBSTANCES IN A PLASTIC STATE
- B29D22/00—Producing hollow articles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B1/00—Layered products having a general shape other than plane
- B32B1/08—Tubular products
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B27/00—Layered products comprising a layer of synthetic resin
- B32B27/06—Layered products comprising a layer of synthetic resin as the main or only constituent of a layer, which is next to another layer of the same or of a different material
- B32B27/08—Layered products comprising a layer of synthetic resin as the main or only constituent of a layer, which is next to another layer of the same or of a different material of synthetic resin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B2250/00—Layers arrangement
- B32B2250/02—2 layers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B2250/00—Layers arrangement
- B32B2250/24—All layers being polymeric
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B2439/00—Containers; Receptacles
- B32B2439/40—Closed containers
- B32B2439/60—Bottles
Abstract
The invention discloses a culture bottle body and a making method thereof. The culture bottle body is of a transparent double-layer structure formed by combining two different plastic materials, wherein one layer is made from thermoplastic plastic for keeping bottle body strength and rigidity, and the other layer is made from airtight plastic. The hot-pressed plastic is nylon or polycarbonate (PC) or polypropylene (PP). The airtight plastic is polyethylene naphthalate (PEN) or polyethylene terephthalate (PET) or polyester or polyvinyl chloride. According to the making method of the culture bottle body, parison is formed first, and then blow molding is performed at the temperature between 25 DEG C and 150 DEG C. Through the making method, complex processes for multilayer plastic bottles are reduced, and cost is lowered; the problems that single-layer plastic bottles cannot have non-deformable strength support and an airtight negative pressure maintaining character simultaneously can be solved, safety risks of glass bottles can be avoided, and weight can be reduced.
Description
Technical field
The present invention relates to culturing bottle, specifically, is a kind of culturing bottle bottle and preparation method thereof.
Background technology
Body fluid cultivate be in human body sample with or without microorganism exist a kind of microbiological test method, comprise hemoculture, Sputum culturing, urine cultivate and other body fluid (hydrothorax, ascites, cerebrospinal fluid etc.) cultivate.Rapid detection seriously being jeopardized clinically to septicemia, the microbemia of patient vitals, whether have microorganism growth with very important effect of clarifying a diagnosis in patient body fluid, is the key of clinical effective treatment.So the detection of pathogenic microorganism should be carried out within the time short as far as possible in body fluid or analogue.In recent years, along with scientific-technical progress, the combination of microbiology, computer and engineering science, has createed the culture systems of many automatizations.
Containing specific substratum and head space blanketing gas in body fluid culturing bottle, and there is certain vacuum tightness, to guarantee collection of body fluids sample smoothly.So body fluid culturing bottle must use applicable gas tightness material, ensure to keep vacuum tightness between effective service life.In addition, the automatic body fluid combinationally used with body fluid culturing bottle cultivates instrument, by light signal collection data, requires that body fluid culturing bottle keeps transparent, to enable detection system read sensor bottom culturing bottle, reaches the object detecting microorganism growth.
Culturing bottle made by the material of current use two type.One type is the vial with plug sealing.The gas tightness of glass itself can well keep the vacuum tightness in bottle, and glass material is transparent, does not affect detection.But glass has intrinsic security risk, if glass breaking can cause operator to be exposed in glass fragment, if the glass culturing bottle with infectious substance used is broken, operator can be caused to be in infection risk.
Another kind of is Plastic Bottle.U.S. Patent number 6123211 describes a kind of laminated plastics bottle, is by internal layer and outer field polycarbonate, provides the strength and stiffness required for culturing bottle purposes.Intermediate materials is manufactured by nylon, makes culturing bottle have air-locked characteristic, but if nylon directly contacts moisture or can not keep transparent through hot-pressing processing.Laminated plastics bottle has advantage at secure context than vial, but its manufacturing process is complicated, and cost is high.
Except complex process in Plastic Bottle, outside the more much higher layer Plastic Bottle of cost, the normal Plastic Bottle using individual layer.The material that monolayer plastic bottle is selected generally mostly is polycarbonate, but polycarbonate has ventilation property, the negative pressure of culturing bottle can not be kept for a long time, cause the inconvenience of injecting sample during use, and ambient atmos is entered after in the culturing bottle of sealing by bottle, the composition of built-in blanketing gas can be changed, may impact microbial growth.Use the monolayer plastic bottle of other materials, then can not meet its intensity again, have distortion when high temperature, break, cause the security risk that infectious substance leaks.
Summary of the invention
Technical problem to be solved by this invention is, provides a kind of technique simple, has support strength and airproof culturing bottle bottle and preparation method thereof.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is: a kind of culturing bottle bottle, the bottle of culturing bottle is the transparent bilayer structure be composited by two kinds of unlike material plastics, wherein one deck is the thermoplastics keeping bottle intensity and rigidity, and another layer is air-locked plastics.
Described thermoplastics is the one in nylon, polycarbonate and polypropylene PP.
Described air-locked plastics are the one in PEN PEN, polyethylene terephthalate PET, polyester and polyvinyl chloride.
Described culturing bottle bottle coordinates elastic gel sealing bottleneck, and does mechanical protection at bottleneck aluminium-plastic cap or plastic cover.
The preparation method of above-mentioned culturing bottle bottle, first forms double-deck parison, then carries out blowing, and the temperature of blowing is at 25 degrees Celsius to 150 degrees Celsius.
The invention has the beneficial effects as follows: reduce laminated plastics bottle complicated technology, reduce costs.Monolayer plastic bottle can be solved again and can not possess indeformable strength support and airproof negative pressure preserving property simultaneously, and security risk and the weight reduction of vial can be avoided.
Accompanying drawing explanation
The structural representation of Fig. 1 culturing bottle bottle of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail:
Principle of work and the prior art of culturing bottle of the present invention are identical, can with reference to prior art, so no longer describe, the present invention and the difference of prior art are that the structure of culturing bottle bottle is different, are described in detail below:
As described in Figure 1, culturing bottle bottle of the present invention, the bottle of culturing bottle is the transparent bilayer structure be composited by two kinds of unlike material plastics, and wherein one deck is the thermoplastics keeping bottle intensity and rigidity, and another layer is air-locked plastics.
Described thermoplastics is the one in nylon, polycarbonate and polypropylene PP.
Described air-locked plastics are the one in PEN PEN, polyethylene terephthalate PET, polyester and polyvinyl chloride.
Described culturing bottle bottle coordinates elastic gel sealing bottleneck, and does mechanical protection at bottleneck aluminium-plastic cap or plastic cover.
The preparation method of above-mentioned culturing bottle bottle, first forms double-deck parison, then carries out blowing, and the temperature of blowing is at 25 degrees Celsius to 150 degrees Celsius.
Culturing bottle of the present invention can be used for body fluid and cultivates, but is not limited to body fluid culturing bottle, and the sample that also may be used for other is cultivated, and comprises clinical sample and non-clinical sample.
Specifically, the internal layer 1 of bottle or outer 2 is made up of the thermoplastics being applicable to of hot pressing process, as nylon, polycarbonate (PC) or polypropylene (PP), keeps intensity and the rigidity of bottle.
Skin 2 or the internal layer 1 of bottle are made up of air-locked plastic material, as PEN (PEN), polyethylene terephthalate (PET), polyester or polyvinyl chloride, keep the gas tightness of bottle.The internal layer of bottle and skin are all transparent materials.
The method of the blowing that the manufacture of bottle uses the simple capital contribution of manufacturing processed low.And there is the specific feature of body fluid culturing bottle needs.
Culturing bottle bottle of the present invention coordinates elastic gel sealing bottleneck, and at bottleneck lid, such as aluminium-plastic cap or plastic cover, do mechanical protection, can form one and airtight transparent have physical strength, prevents from breaking and the culturing bottle of thermal distortion.
In implementation process, first form double-deck parison, then carry out blowing.The temperature of blowing is at 25 degrees Celsius to 150 degrees Celsius.Double-deck parison, according to the difference of material, can adopt different processing steps, such as, by good for bed device outside mold core and parison mould, first injects bottle inner layer material polycarbonate, temperature 120 DEG C from mold bottom.Material feeding completes rear mold inner core and leads to cooling water condensation.Together take out together with mold core after inner layer material condensation, put into second parison mould, injecting outer layer material polyethylene terephthalate, temperature 100 DEG C.Mould adobe blocks after cladding material condensation, parison is taken out and puts into double bottle mould, adopt general hot blow molding process to carry out blow molding.Open mould, finishing overlap, obtains double bottle.
Double-deck bottle of the present invention, the material that bottle uses meets the requirement of culturing bottle needs: keep transparency, to facilitate the sensor bottom body fluid cultivation instrument detection system reading culturing bottle, selects transparent material to manufacture, and not by the impact of hot-pressing processing; Keep gas tightness, to keep the negative pressure in culturing bottle, and make the built-in gaseous constituent in bottle not be subject to the interference of ambient atmos, to ensure between effective service life normal injecting sample and carry out microorganism culturing, wherein one deck of double bottle selects air-locked plastic material, as polyethylene terephthalate or PEN; Keep the support of bottle and intensity, than prevent from falling or high heat time break or be out of shape, wherein one deck selection pressure-and heat-resistance of double bottle has the material compared with hard intensity and rigidity, preferably polycarbonate.Such as having the body fluid culturing bottle of biosensor, biosensor composition is joined in bottle; The sterilized substratum provided by microorganism growth joins in bottle; Sterilizing elastic plug is placed in bottleneck; Atmosphere surrounding and vacuum tightness in bottle is formed by filling apparatus; By elastic plug compacting; Bottle cap is wrapped plug and bottleneck, and compacting.
Above-described embodiment is only for illustration of technological thought of the present invention and feature, its object is to enable those skilled in the art understand content of the present invention and implement according to this, only can not limit the scope of the claims of the present invention with the present embodiment, namely the equal change done of all disclosed spirit or modification, still drop in the scope of the claims of the present invention.
Claims (5)
1. a culturing bottle bottle, is characterized in that, the bottle of culturing bottle is the transparent bilayer structure be composited by two kinds of unlike material plastics, and wherein one deck is the thermoplastics keeping bottle intensity and rigidity, and another layer is air-locked plastics.
2. culturing bottle bottle according to claim 1, is characterized in that, described thermoplastics is the one in nylon, polycarbonate and polypropylene PP.
3. culturing bottle bottle according to claim 1, is characterized in that, described air-locked plastics are the one in PEN PEN, polyethylene terephthalate PET, polyester and polyvinyl chloride.
4. culturing bottle bottle according to claim 1, is characterized in that, described culturing bottle bottle coordinates elastic gel sealing bottleneck, and does mechanical protection at bottleneck aluminium-plastic cap or plastic cover.
5. the preparation method of culturing bottle bottle as claimed in claim 1, it is characterized in that, first form double-deck parison, then carry out blowing, the temperature of blowing is at 25 degrees Celsius to 150 degrees Celsius.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610090972.3A CN105543070A (en) | 2016-02-18 | 2016-02-18 | Culture bottle body and making method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610090972.3A CN105543070A (en) | 2016-02-18 | 2016-02-18 | Culture bottle body and making method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105543070A true CN105543070A (en) | 2016-05-04 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610090972.3A Pending CN105543070A (en) | 2016-02-18 | 2016-02-18 | Culture bottle body and making method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105543070A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85101400A (en) * | 1985-04-01 | 1987-01-31 | 日精Asb机械公司 | Twin shaft with excellent heat resistance energy and airtight performance is to plastic container |
| CN1516642A (en) * | 2001-06-18 | 2004-07-28 | ���˶ٵϿ�ɭ��˾ | Multilayer containers and procss for forming multilayer containers |
| CA2774412A1 (en) * | 2009-10-02 | 2011-04-07 | Biomerieux, Inc. | Single layer plastic test sample culture bottle |
| CN103662277A (en) * | 2012-09-10 | 2014-03-26 | 徐跃 | Double-layer polyester bottle and forming technology thereof |
| CN104837602A (en) * | 2012-10-31 | 2015-08-12 | 生物梅里埃有限公司 | Aseptic blow, fill and seal methods of fabricating test sample containers and associated systems and containers |
| CN205528762U (en) * | 2016-02-18 | 2016-08-31 | 允汇科技（天津）有限公司 | Blake bottle bottle |
-
2016
- 2016-02-18 CN CN201610090972.3A patent/CN105543070A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85101400A (en) * | 1985-04-01 | 1987-01-31 | 日精Asb机械公司 | Twin shaft with excellent heat resistance energy and airtight performance is to plastic container |
| CN1516642A (en) * | 2001-06-18 | 2004-07-28 | ���˶ٵϿ�ɭ��˾ | Multilayer containers and procss for forming multilayer containers |
| CA2774412A1 (en) * | 2009-10-02 | 2011-04-07 | Biomerieux, Inc. | Single layer plastic test sample culture bottle |
| CN103662277A (en) * | 2012-09-10 | 2014-03-26 | 徐跃 | Double-layer polyester bottle and forming technology thereof |
| CN104837602A (en) * | 2012-10-31 | 2015-08-12 | 生物梅里埃有限公司 | Aseptic blow, fill and seal methods of fabricating test sample containers and associated systems and containers |
| CN205528762U (en) * | 2016-02-18 | 2016-08-31 | 允汇科技（天津）有限公司 | Blake bottle bottle |
Non-Patent Citations (1)
| Title |
|---|
| 邹恩广等主编: "《塑料制品加工技术》", 30 April 2008, 中国纺织出版社 * |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160504 |
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| RJ01 | Rejection of invention patent application after publication |