CN105535476A - 一种检验科外伤用杀菌消炎药剂及制备方法 - Google Patents
一种检验科外伤用杀菌消炎药剂及制备方法 Download PDFInfo
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Abstract
本发明涉及一种检验科外伤用杀菌消炎药剂及制备方法，制成所述药剂有效成分的原料组成为：节节花、贝母兰、蛇含、红背叶、毛稔、树萝卜、柳根、朱砂根、小蜡树、罗勒、树茭瓜、姬蕨、老龙皮、蒲黄、竹叶蕉和地星；本发明药剂所选药材配伍相宜，诸药相和具有祛风止痛、清热解毒、消炎抗菌等优点，且对人体皮肤无刺激，适用于检验科用外伤伤口标本提取的杀菌消炎，也可用于皮肤以及粘膜的消毒、皮肤外伤消毒，值得检验科广泛推广应用。
Description
技术领域
本发明涉及中药领域，特别涉及一种检验科外伤用杀菌消炎药剂及制备方法。
背景技术
检验科是临床医学和基础医学之间的桥梁，包括临床化学、临床微生物学、临床免疫学、血液学、体液学以及输血学等分支学科。
检验科作为医院检验化验的科室，经常会对一些外伤患者提取标本进行化验，而化验所使用的杀菌消炎药剂如碘酒、酒精、碘伏等大多对人体伤口以及皮肤有刺激性，不利于伤口愈合，有的甚至会引起过敏反应，若杀菌消炎不彻底，很容易使伤口化脓，影响患者病情好转和正常的生活。
所以，为外伤患者提供安全保证，也为检验科人员工作提供方便，我们需要寻找一种杀菌消炎效果好且对皮肤无刺激性的药剂，来解决上述问题。
发明内容
本发明提供了一种检验科外伤用杀菌消炎药剂及制备方法；本发明药剂所选药材配伍相宜，诸药相和具有祛风止痛、清热解毒、消炎抗菌等优点，且对人体皮肤无刺激，适用于检验科用外伤伤口标本提取的杀菌消炎，也可用于皮肤以及粘膜的消毒、皮肤外伤消毒，值得检验科广泛推广应用。
为实现上述目的，本发明的技术方案实施如下：
一种检验科外伤用杀菌消炎药剂及制备方法，制成所述药剂有效成分的原料组成及重量份数为：
节节花59-89份贝母兰57-87份蛇含57-87份红背叶54-82份
毛稔52-80份树萝卜52-80份柳根47-77份朱砂根47-77份
小蜡树42-70份罗勒42-70份树茭瓜40-68份姬蕨37-64份
老龙皮37-64份蒲黄32-57份竹叶蕉32-57份地星22-42份。
制成所述药剂有效成分的原料组成及重量份数为：
节节花80份贝母兰78份蛇含78份红背叶74份
毛稔72份树萝卜72份柳根67份朱砂根67份
小蜡树60份罗勒60份树茭瓜58份姬蕨55份
老龙皮55份蒲黄47份竹叶蕉47份地星35份。
制成所述药剂有效成分的原料组成及重量份数为：
节节花73份贝母兰71份蛇含71份红背叶66份
毛稔64份树萝卜64份柳根61份朱砂根61份
小蜡树54份罗勒54份树茭瓜52份姬蕨49份
老龙皮49份蒲黄42份竹叶蕉42份地星31份。
制成所述药剂有效成分的原料组成及重量份数为：
节节花66份贝母兰64份蛇含64份红背叶60份
毛稔58份树萝卜58份柳根55份朱砂根55份
小蜡树48份罗勒48份树茭瓜46份姬蕨43份
老龙皮43份蒲黄37份竹叶蕉37份地星26份。
一种检验科外伤用杀菌消炎药剂的制备方法，包括以下工艺步骤：
（1）将节节花、蛇含、红背叶、毛稔、树萝卜和小蜡树放入容器中，加入10倍量蒸馏水浸泡4小时后，加热煮沸4小时，提取；第二次加入8倍量蒸馏水加热煮沸3小时，提取；第三次加入6倍量蒸馏水加热煮沸2小时，提取；合并三次所得提取液，滤过，得滤液备用；
（2）将贝母兰、姬蕨、老龙皮、蒲黄和地星微波烘干，研末，得到过200目的细粉，备用；
（3）将柳根研末后，浸入体积浓度为90%酒精溶液，静置4小时，取出上层清液，沉淀下来的浸膏，备用；
（4）将朱砂根、罗勒、树茭瓜和竹叶蕉绞汁，滤杂质，留汁液，备用；
（5）将上述步骤（1）-（4）所制的滤液、细粉、浸膏以及汁液混合，并加入10-12倍量的蒸馏水，搅拌溶解均匀后，加热煮沸1小时，置于60℃以下，静置1小时，过滤，得滤液，将滤液灌装封存，121℃热压灭菌30分钟，包装，即为所述的检验科外伤用杀菌消炎药剂的制备方法。
本发明所用中药的药性如下：
节节花：味甘，性寒；归心、胃、小肠经。凉血散瘀，清热解毒，除湿通淋。主咳血，吐血，便血，湿热黄疸，痢疾，泄泻，牙龈肿痛，咽喉肿痛，肠痈，乳痈，痒腮，痈疽肿毒，湿疹，淋症，跌打损伤，毒蛇咬伤。《福建民间草药》载：“消炎止痛，拔毒退肿。”
贝母兰：味辛、甘，性凉；归肺、肾经。清热止咳，止血定痛。主支气管炎，感冒，骨折，软组织挫伤，外伤出血。《文山中草药》载：“止血，定痛。治外伤出血。”
蛇含：味苦，性寒；归经肝、肺经。清热定惊，截疟，止咳化痰，解毒活血。主高热惊风，疟疾，肺热咳嗽，百晶咳，痢疾，疮疖肿毒，咽喉肿痛，风火牙痛，带状疱疹，目赤肿痛，虫蛇咬伤，风湿麻木，跌打损伤，月经不调，外伤出血。
红背叶：味甘，性凉；归膀胱、大肠、肺经。清热利湿，凉血解毒，杀虫止痒。主痢疾，热淋，石淋，血尿，崩漏，带下，风疹，湿疹，疥癣，龋齿痛，褥疮。《广西中草药》载：“解毒，除湿，止血。治痢疾，尿路结石或炎症，血崩，白带，风疹，疥疮，脚癣，龋齿痛，外伤出血。”现代医学研究证实，本品试管试验只对金黄色与白色葡萄球菌有轻度抑制作用。
毛稔：味苦、涩，性凉；归脾、肝经。解毒止痛，生肌止血。主痧气腹痛，痢疾，便血，月经过多，疮疖，跌打肿痛，外伤出血。广州部队《常用中草药手册》载：“收敛止血，消食止痢。根：治水泻，便血，妇女月经过多。叶：治外伤出血。”
树萝卜：味微苦，性凉；归脾经。利水消肿，活血祛瘀。主水肿，跌打损伤。
柳根：味苦，性寒。利水通淋，祛风除痛，泻火解毒。主淋证，白浊，水肿，
黄疸，痢疾，白带，风湿疼痛，黄水疮，牙痛，烫伤，乳痈。《纲目》载：“煎服，治黄疸，白浊；酒煮，熨诸痛肿，去风，止痛消肿。”
朱砂根：味苦、辛，性凉。清热解毒，活血止痛。主咽喉肿痛，风湿热痹，黄疸，痢疾，跌打损伤，流火，乳腺炎，睾丸炎。《生草药性备要》载：“治痰火，跌打，去瘀生新，宽筋续骨。”
小蜡树：味苦，性凉。清热利湿，解毒消肿。主感冒发热，肺热咳嗽，咽喉肿痛，口舌生疮，湿热黄疸，痢疾，痈肿疮毒，湿疹，皮炎，跌打损伤，烫伤。
罗勒：味辛、甘，性温；归肺、脾、胃、大肠经。疏风解表，化湿和中，行气活血，解毒消肿。主感冒头痛，发热咳嗽，中暑，食积不化，不思饮食，脘腹胀满疼痛，哎吐泻痢，风湿痹痛，遗精，月经不调，牙痛口臭，胬肉遮睛，皮肤湿疮，瘾疹瘙痒，跌打损伤，蛇虫咬伤。广州部队《常用中草药手册》载：“消肿，散瘀止痛。治胃肠胀气，消化不良，胃痛，肠炎腹泻，外感风寒，头痛，胸痛，跌打瘀肿，风湿痹痛，湿疹皮炎。”
树茭瓜：甘淡，平。清热润肺，化痰止咳，散瘀止血。治肺结核，肺炎，气管、支气管炎，咽喉炎，月经不调，骨折筋伤，外伤出血。《云南思茅中草药选》载：“止咳化痰，散瘀消肿，止血消炎。治肺结核，肺炎，气管、支气管炎，喘咳，骨折筋伤。”
姬蕨：味苦、辛，性凉；归肺、肝经。清热解毒，收敛止血。主烧烫伤，外伤出血。《新华本草纲要》载：“有清热解毒，收敛止血的功能。用于烫伤及外伤出血。”
老龙皮：味淡、微苦，性平；归脾、肾经。消食健脾，利水消肿，祛风止痒。主消化不良，小儿疳积，腹胀，水肿，皮肤瘙痒，无名肿毒。
蒲黄：味甘、微辛，性平；归肝、心、脾经。止血，祛瘀，利尿。主吐血，咯血，衄血，备痢，便血，崩漏，外伤出血，心腹疼痛，经闭腹痛，产后瘀痛，痛经，跌扑肿痛，血淋涩痛，带下，重舌，口疮，聤耳，阴下湿痒。《本经》载：“主心腹膀脱寒热，利小便，止血，消瘀血。”
竹叶蕉：味淡，性凉；归肺经。清热解毒，止咳定喘。主感冒发热，咳嗽，小儿麻疹合并肺炎，哮喘，肺结核。
地星：味辛，性平。清肺利咽，解毒消肿，止血。主咳嗽，咽喉肿痛，痈肿疮毒，冻疮流水，吐血，衄血，外伤出血。
本发明与现有技术相比，具有以下有益效果：
本发明药剂所选药材配伍相宜，诸药相和具有祛风止痛、清热解毒、消炎抗菌等优点，且对人体皮肤无刺激，适用于检验科用外伤伤口标本提取的杀菌消炎，也可用于皮肤以及粘膜的消毒、皮肤外伤消毒，值得检验科广泛推广应用。
具体实施方式
下面结合具体实施例对本发明做进一步阐述。
实施例1
本发明药剂有效成分的原料组成及重量为：
节节花87g贝母兰85g蛇含85g红背叶80g
毛稔78g树萝卜78g柳根75g朱砂根75g
小蜡树68g罗勒68g树茭瓜66g姬蕨62g
老龙皮62g蒲黄55g竹叶蕉55g地星40g。
一种检验科外伤用杀菌消炎药剂的制备方法，包括以下工艺步骤：
（1）将节节花、蛇含、红背叶、毛稔、树萝卜和小蜡树放入容器中，加入10倍量蒸馏水浸泡4小时后，加热煮沸4小时，提取；第二次加入8倍量蒸馏水加热煮沸3小时，提取；第三次加入6倍量蒸馏水加热煮沸2小时，提取；合并三次所得提取液，滤过，得滤液备用；
（2）将贝母兰、姬蕨、老龙皮、蒲黄和地星微波烘干，研末，得到过200目的细粉，备用；
（3）将柳根研末后，浸入体积浓度为90%酒精溶液，静置4小时，取出上层清液，沉淀下来的浸膏，备用；
（4）将朱砂根、罗勒、树茭瓜和竹叶蕉绞汁，滤杂质，留汁液，备用；
（5）将上述步骤（1）-（4）所制的滤液、细粉、浸膏以及汁液混合，并加入10-12倍量的蒸馏水，搅拌溶解均匀后，加热煮沸1小时，置于60℃以下，静置1小时，过滤，得滤液，将滤液灌装封存，121℃热压灭菌30分钟，包装，即为所述的检验科外伤用杀菌消炎药剂的制备方法。
实施例2
本发明药剂有效成分的原料组成及重量为：
节节花80g贝母兰78g蛇含78g红背叶74g
毛稔72g树萝卜72g柳根67g朱砂根67g
小蜡树60g罗勒60g树茭瓜58g姬蕨55g
老龙皮55g蒲黄47g竹叶蕉47g地星35g。
本实施例药剂制备方法工艺步骤与实施例1相同。
实施例3
本发明药剂有效成分的原料组成及重量为：
节节花73g贝母兰71g蛇含71g红背叶66g
毛稔64g树萝卜64g柳根61g朱砂根61g
小蜡树54g罗勒54g树茭瓜52g姬蕨49g
老龙皮49g蒲黄42g竹叶蕉42g地星31g。
本实施例药剂制备方法工艺步骤与实施例1相同。
实施例4
本发明药剂有效成分的原料组成及重量为：
节节花66g贝母兰64g蛇含64g红背叶60g
毛稔58g树萝卜58g柳根55g朱砂根55g
小蜡树48g罗勒48g树茭瓜46g姬蕨43g
老龙皮43g蒲黄37g竹叶蕉37g地星26g。
本实施例药剂制备方法工艺步骤与实施例1相同。
抑菌实验
1、实验菌种：
挑选实验室具有代表性的菌种，金黄色葡萄球菌、绿脓杆菌和白喉杆菌。
2、菌种培养基：
营养琼脂培养基和营养肉汤培养基。
3、实验分类：
抑菌实验分实验组和对照组，实验组选用本发明药剂，对照组选用碘伏。
4、具体实验步骤：
（1）菌液的制备：将上述三种菌种分别接种于三个琼脂培养基上，在37℃下培养24小时，分别从上述琼脂培养基中挑取典型菌落分别接种于三个新鲜的营养肉汤培养基中，同温度下，培养18小时，备用；
（2）取本发明消毒剂和碘伏各50ml，分别置于稀释容器中，对其分别稀释30倍，将上述两种稀释液溶液分别分置三个容器内，置于无菌条件下，备用；
（3）将上述步骤（1）经营养肉汤培养22小时的实验菌种用无菌的营养肉汤分别稀释50倍，每种菌种分装于两个培养皿中，分别标记对照组和实验组；
（4）将上述步骤（2）所得的两份本发明药剂的稀释溶液分别滴入实验组培养皿，三份碘伏稀释溶液分别滴入对照组培养皿。
5、试验观察测量：
对上述实验进行观察，观察两组菌种并测量菌种残留面积具体结果。
6、观测测量完毕后，将上述培养皿均置于无菌条件下静置两天，待药剂完全挥发后，跟踪观察发现，对照组菌落继续繁殖，而实验组菌落无变化。
7、实验结果：
实验组本发明药剂的杀菌能力远大于对照组药剂的杀菌能力，并且能够抑制各类菌种继续繁殖。
Claims (5)
1.一种检验科外伤用杀菌消炎药剂及制备方法，其特征在于：制成所述药剂有效成分的原料组成及重量份数为：
节节花59-89份贝母兰57-87份蛇含57-87份红背叶54-82份
毛稔52-80份树萝卜52-80份柳根47-77份朱砂根47-77份
小蜡树42-70份罗勒42-70份树茭瓜40-68份姬蕨37-64份
老龙皮37-64份蒲黄32-57份竹叶蕉32-57份地星22-42份。
2.根据权利要求1所述的一种检验科外伤用杀菌消炎药剂及制备方法，其特征在于：制成所述药剂有效成分的原料组成及重量份数为：
节节花80份贝母兰78份蛇含78份红背叶74份
毛稔72份树萝卜72份柳根67份朱砂根67份
小蜡树60份罗勒60份树茭瓜58份姬蕨55份
老龙皮55份蒲黄47份竹叶蕉47份地星35份。
3.根据权利要求1所述的一种检验科外伤用杀菌消炎药剂及制备方法，其特征在于：制成所述药剂有效成分的原料组成及重量份数为：
节节花73份贝母兰71份蛇含71份红背叶66份
毛稔64份树萝卜64份柳根61份朱砂根61份
小蜡树54份罗勒54份树茭瓜52份姬蕨49份
老龙皮49份蒲黄42份竹叶蕉42份地星31份。
4.根据权利要求1所述的一种检验科外伤用杀菌消炎药剂及制备方法，其特征在于：制成所述药剂有效成分的原料组成及重量份数为：
节节花66份贝母兰64份蛇含64份红背叶60份
毛稔58份树萝卜58份柳根55份朱砂根55份
小蜡树48份罗勒48份树茭瓜46份姬蕨43份
老龙皮43份蒲黄37份竹叶蕉37份地星26份。
5.一种如权利要求1-4任一项所述检验科外伤用杀菌消炎药剂的制备方法，其特征在于：包括以下工艺步骤：
（1）将节节花、蛇含、红背叶、毛稔、树萝卜和小蜡树放入容器中，加入10倍量蒸馏水浸泡4小时后，加热煮沸4小时，提取；第二次加入8倍量蒸馏水加热煮沸3小时，提取；第三次加入6倍量蒸馏水加热煮沸2小时，提取；合并三次所得提取液，滤过，得滤液备用；
（2）将贝母兰、姬蕨、老龙皮、蒲黄和地星微波烘干，研末，得到过200目的细粉，备用；
（3）将柳根研末后，浸入体积浓度为90%酒精溶液，静置4小时，取出上层清液，沉淀下来的浸膏，备用；
（4）将朱砂根、罗勒、树茭瓜和竹叶蕉绞汁，滤杂质，留汁液，备用；
（5）将上述步骤（1）-（4）所制的滤液、细粉、浸膏以及汁液混合，并加入10-12倍量的蒸馏水，搅拌溶解均匀后，加热煮沸1小时，置于60℃以下，静置1小时，过滤，得滤液，将滤液灌装封存，121℃热压灭菌30分钟，包装，即为所述的检验科外伤用杀菌消炎药剂的制备方法。
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