CN105525361B - It is a kind of for complex enzyme formulation of flax fiber raw material degumming and preparation method thereof and flax fiber raw material Degumming method - Google Patents

It is a kind of for complex enzyme formulation of flax fiber raw material degumming and preparation method thereof and flax fiber raw material Degumming method Download PDF

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CN105525361B
CN105525361B CN201610079747.XA CN201610079747A CN105525361B CN 105525361 B CN105525361 B CN 105525361B CN 201610079747 A CN201610079747 A CN 201610079747A CN 105525361 B CN105525361 B CN 105525361B
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alkaline
enzyme
raw material
alkalescent xylanase
flax fiber
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CN105525361A (en
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程海泳
郝志军
庄冉
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Nanning Yuanshi Biotechnology Co.,Ltd.
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Jiaxing Zhuosheng Biological Science & Technology Co Ltd
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    • DTEXTILES; PAPER
    • D01NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01CCHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
    • D01C1/00Treatment of vegetable material

Abstract

The invention discloses a kind of for complex enzyme formulation of flax fiber raw material degumming and preparation method thereof and flax fiber raw material Degumming method, the complex enzyme formulation includes alkaline pectate lyase, alkali cellulose enzyme, alkalescent xylanase and laccase, and weight/mass percentage composition of each component in complex enzyme formulation is as follows:Alkaline pectate lyase:70 ﹪;Alkali cellulose enzyme:2 ﹪;Alkalescent xylanase:18 ﹪;Laccase:10%.Using technical scheme, in scouring processes, bleaching liquor consumption more than 20% during bleaching flax fibers is reduced;Reduce amount of caustic soda more than 40% during bleaching flax fibers;Flax fiber fracture strength increase by more than 5%, fineness increase by more than 20%, elongation at break increase by more than 30%, fibre strength is uniform so that the spinnability of fiber is obviously improved, so as to increase economic efficiency, reduces production cost;COD reductions by more than 30%.

Description

It is a kind of for complex enzyme formulation of flax fiber raw material degumming and preparation method thereof and Flax fiber raw material Degumming method
Technical field
The invention belongs to textile technology field, it is related to flax fiber raw material degumming, more particularly to it is a kind of for flax fiber Complex enzyme formulation of raw material degumming and preparation method thereof and flax fiber raw material Degumming method.
Background technology
Flax fiber is the fine fiber in textile industry, its fabric not only moisture absorption good heat dissipation, and gas permeability is good, and has Well-pressed natural, the comfortable and easy to wear style such as nice and cool, is loved by consumers.Its fiber-spinnable is present in be existed in the form of fibre bundle In the tough intracutaneous of flax straw, the single fiber length of flax is 10mm~26mm, single fiber two ends by pectic substance axially overlap joint or Lateral switching forms fibre bundle, between fibre bundle and fibre bundle, between bast and xylem is linked by colloid.In order to make Take the fiber-spinnable in flax, it is necessary to remove the miscellaneous composition such as the glue in bast, this technical process is referred to as flax straw and taken off Glue.Using strong in chemical degumming technique of traditional flax fiber plant degumming tech substantially based on caustic soda, the technical process The method of sour highly basic, HTHP, with pollution is big, high energy consumption, low row yielding, production cycle length, water consumption are big, destruction fiber A series of problems, such as fracture strength, severe working environment, especially, the waste water that the degumming of flax fiber Phytochemistry is produced, have concentration The features such as high, colourity is high, basicity is high, its complicated component, and containing the material for being largely difficult to degrade, carry out the difficulty of environmental protection treatment It is very big, seriously govern the development of Fiber Flax Industry.
Enzyme degumming technology is the catalytic action selectivity by biology enzyme, by cellulose and hemicellulose, lignin, pectin Deng separation, so as to obtain high-quality flax fiber and greatly reduce environmental pollution.Because it substitutes original technique, pollution treatment effect Fruit significantly, is paid close attention in many ways.Although the enzyme degumming technology research of its flax fiber in recent years obtains certain progress, big absolutely Majority is in the laboratory research stage, it is impossible to obtain commercial application.Main reason is that the 1, unstability of enzyme preparation structure, How corresponding enzyme selection, accurate control, incorporate in flax fiber degumming tech, still suffer from more problem;2nd, commercially available enzyme Vigor is relatively low and price is higher, and 3, the consumption of enzymatic reagent not science;If enzyme concentration is few in scouring processes, residual gum content does not reach Mark;If enzyme concentration is big, production cost is not dominant, and has obvious damage to flax fiber fracture strength;4th, enzymatic structure Unreasonable, such as, current flax degumming is main based on pectase with enzyme preparation, due to the selectivity of enzyme preparation, its just for Pectic substance tires out material and is hydrolyzed, and the material such as hemicellulose, lignin, paraffin paper class can only be gone by certain density soda acid Remove, certainly will so influence fiber fracture strength and can anti-property.Therefore, from the aspect of quality, cost two, existing enzyme degumming Method is difficult popularization and application, is to use the enzymatic degumming of half chemical half mostly, can not still solve that degumming waste water amount is big, COD value is high, The costly problem of environmental protection treatment.
Therefore, for drawbacks described above present in currently available technology, it is necessary to be studied in fact, to provide a kind of scheme, Solve defect present in prior art.
The content of the invention
In view of this, it is a kind of for complex enzyme formulation of flax fiber raw material degumming and preparation method thereof it is necessory to provide And flax fiber raw material Degumming method, cost is low, technique is simple and is suitable for industrial applications.
In order to overcome the shortcomings of the prior art, the present invention provides following technical scheme:
A kind of complex enzyme formulation for flax fiber raw material degumming, including alkaline pectate lyase, alkali cellulose Enzyme, alkalescent xylanase and laccase, weight/mass percentage composition of each component in complex enzyme formulation are as follows:
Alkaline pectate lyase:70 ﹪;
Alkali cellulose enzyme:2 ﹪;
Alkalescent xylanase:18 ﹪;
Laccase:10%.
Preferably, basic poly galacturonic acid enzyme and alkaline pectin ester are also included in the alkaline pectate lyase Enzyme, weight/mass percentage composition is as follows:
Alkaline pectate lyase:70 ﹪;
Basic poly galacturonic acid enzyme:15%;
Alkaline pectin esterase:15%.
Preferably, the alkali cellulose enzyme include circumscribed alkali cellulose enzyme, alkaline endo cellulase and alkalescence β- Dextranase, weight/mass percentage composition is as follows:
Circumscribed alkali cellulose enzyme:60 ﹪;
Alkaline endo cellulase:30 ﹪;
Alkaline 1,4 beta-glucanase:10%.
Preferably, the alkalescent xylanase includes inscribe alkalescent xylanase and circumscribed alkalescent xylanase, quality hundred Divide content as follows:
The ﹪ of inscribe alkalescent xylanase 80;
The circumscribed ﹪ of alkalescent xylanase 20.
The invention also discloses a kind of flax fiber raw material Degumming method, comprise the following steps:
Step (1):Flax fiber raw material, complex enzyme formulation and water are well mixed in bucking kier;
Step (2):Flax fiber raw material after step (1) enzyme effect is washed and obtains flax through bleaching process again Fiber;
Wherein, the complex enzyme formulation mass percent is the 1 ‰ to 1.5 ‰ of flax fiber raw material;The complex enzyme system Agent includes 70 ﹪ alkaline pectate lyase, 2 ﹪ alkali cellulose enzyme, 18 ﹪ alkalescent xylanase and 10% paint Enzyme, said components ratio is mass percent:
During the enzyme effect of step (1), temperature maintains 50 DEG C~60 DEG C, and pH value maintains 7.0~8.5, degumming Time is 2h~4h.
Preferably, hydrogen peroxide bleaching process is used in the step (2).
Preferably, in the step (1), add soda ash adjustment pH value and pH value is more than 6.5 after effect terminates.
Preferably, in the step (1), water circulation is carried out by circulating pump and persistently stirred.
Preferably, basic poly galacturonic acid enzyme and alkaline pectin ester are also included in the alkaline pectate lyase Enzyme, weight/mass percentage composition is as follows:
Alkaline pectate lyase:70 ﹪;
Basic poly galacturonic acid enzyme:15%;
Alkaline pectin esterase:15%;
The alkali cellulose enzyme includes circumscribed alkali cellulose enzyme, alkaline endo cellulase and alkaline beta glucan Enzyme, weight/mass percentage composition is as follows:
Circumscribed alkali cellulose enzyme:60 ﹪;
Alkaline endo cellulase:30 ﹪;
Alkaline 1,4 beta-glucanase:10%;
The alkalescent xylanase includes inscribe alkalescent xylanase and circumscribed alkalescent xylanase, and weight/mass percentage composition is such as Under:
The ﹪ of inscribe alkalescent xylanase 80;
The circumscribed ﹪ of alkalescent xylanase 20.
The invention also discloses a kind of preparation method of the complex enzyme formulation for flax fiber raw material degumming, including it is following Step:
Step (1):By raw material enzyme preparation alkaline pectate lyase, alkali cellulose enzyme, alkalescent xylanase and laccase Pre-treatment is carried out respectively;
Step (2):It is 70 ﹪ alkaline pectate lyase, 2 ﹪ alkali cellulose enzyme, 18 ﹪ by mass percent Alkalescent xylanase and 10% laccase are well mixed in blender;
Step (3):Then add appropriate preservative and carried out by diatomite filter it is degerming, then by determining after enzyme activity Prepare the complex enzyme formulation for flax fiber raw material degumming.
Compared with prior art, possesses following technique effect using technical scheme:
1st, using transelminase, cellulase and zytase can direct hydrolysis pectic substance, xylan, Portugal gather The class material such as sugar, heteroglycan, so as to discharge fibre bundle so that bast and xylem are easily isolated, recycles laccase hydrolysis separation Lignin afterwards;
2nd, using hydrogen peroxide bleaching process, while reaching that the Baidu of spinning process requirement requires, the high pH of oxygen bleaching is utilized The operational characteristic of value and high temperature, is further removed to cull, so as to reach the purpose of flax fiber raw material degumming;
3rd, bleaching liquor consumption more than 20% during bleaching flax fibers is reduced;
4th, amount of caustic soda more than 40% during bleaching flax fibers is reduced;
5th, flax fiber fracture strength increase by more than 5%, fineness increase by more than 20%, elongation at break increase by 30% with On, fibre strength is uniform so that the spinnability of fiber is obviously improved, so as to increase economic efficiency, reduces production cost;
6th, the color of waste water is improved as present light brown or yellow by original black or dark brown, COD reductions by 30% with On.
Embodiment
The invention will be further described below.
In order to overcome the defect of prior art, applicant is to the morphosis of flax bast fiber, chemical composition and micro- Structure is seen to conduct in-depth research.
Ultimate flax fibre is primary phloem fiber cell, and a cell is exactly a single fiber, and single fiber average length is 10~26mm.Because individual fiber cells are shorter, length is uneven, and flax fiber can only be spinned with process of fiber state.It is sub- The bundles of bast part for being distributed in stem of flaxen fiber, therefore also referred to as bast fiber.Radially there are 20~40 fibers in straw Beam is uniformly distributed, in the circular fibrous layer that a circle is complete.Fibre bundle, which is formed, continuously to be passed through full stem, is laterally then mutually interconnected around full stem The network structure of knot.Isolated between fibre bundle and fibre bundle by parenchyma cell.It is exactly to destroy this portion during flax straw degumming Divide parenchyma cell and isolate fiber one by one, form the fibre bundle being partially separated.
Flax fiber is main to be graded material composition, cellulose by cellulose, hemicellulose, lignin, pectin, wax and ash Content height decide the quality of fiber quality.Pectin is the nutrients of plant generation cellulose, hemicellulose and lignin Matter.Because the presence of pectin makes fibrocyte be sticked together each other in fiber, make that between fiber relative shifting can not be produced Dynamic, this is unfavorable to spinning.Hemicellulose level and flax fiber, yarn, the spinning property of fabric, yarn forming performance and fabric Wearability is also closely related.The poor quality of the too high levels of hemicellulose then fiber in flaxen fiber, it is on the contrary then better.Therefore During flax degumming, pectin and hemicellulose are the main objects removed.
The microstructure of flax fiber is primarily referred to as the aggregated structure of cellulose macromolecule, in other words cellulose macromolecule Ordered state, orientation, aggregation tightness degree etc..The basic composition cellulose of flax fiber, is much glucose The macromolecular chain that residue is connected.At a certain distance, a phase bit, certain relative shape comparison rule and it is stably bound in Together, as crystalline state, very thin macromolecular beam, as base are fine.Linear molecule chain is parallel to each other, former by some foundation It is fine it is arranged in parallel is combined together, formed it is relatively thick any be substantially in crystalline state micro- fibril macromolecular beam.Some micro- originals Fibre is combined together to form fibril.Thicker macromolecular beam is obtained for giant fibril by substantially parallel the piling up of fibril, crudefiber crop it is huge Fibrillation is a cell, is finally piled into fiber by giant fibril.Divide in the gap of the microstructures at different levels of flax and hole Cloth the accompaniments such as pectin, hemicellulose, lignin.
As pointed by background technology, the unstability of prior art enzyme preparation structure, corresponding enzyme selection, essence Really how control, incorporate in flax fiber degumming tech, still suffer from more problem;Such as, current flax degumming uses enzyme preparation master Will be based on pectase, however, pectase is also classified into many kinds, the degumming effect of every kind of enzyme is differed;Simultaneously because enzyme system The selectivity of agent, pectase tires out material just for pectic substance and is hydrolyzed, and makes degumming effect not good.
On the basis conducted in-depth research to the morphosis of flax bast fiber, chemical composition and microstructure On, and the in test measure to various enzyme performances, applicant proposed a kind of for the compound of flax fiber raw material degumming Enzyme preparation, including alkaline pectate lyase, alkali cellulose enzyme, alkalescent xylanase and laccase, each component is in complex enzyme system Weight/mass percentage composition in agent is as follows:
Alkaline pectate lyase:70 ﹪;
Alkali cellulose enzyme:2 ﹪;
Alkalescent xylanase:18 ﹪;
Laccase:10%.
Further, basic poly galacturonic acid enzyme and alkaline pectin esterase are also included in alkaline pectate lyase, Weight/mass percentage composition is as follows:
Alkaline pectate lyase:70 ﹪;
Basic poly galacturonic acid enzyme:15%;
Alkaline pectin esterase:15%.
Further, alkali cellulose enzyme includes circumscribed alkali cellulose enzyme, alkaline endo cellulase and alkaline β-Portugal Dextranase, weight/mass percentage composition is as follows:
Circumscribed alkali cellulose enzyme:60 ﹪;
Alkaline endo cellulase:30 ﹪;
Alkaline 1,4 beta-glucanase:10%.
Further, alkalescent xylanase includes inscribe alkalescent xylanase and circumscribed alkalescent xylanase, quality percentage Content is as follows:
The ﹪ of inscribe alkalescent xylanase 80;
The circumscribed ﹪ of alkalescent xylanase 20.
The principle of above-mentioned various enzymes has following distinguishing feature:
The adhesion material of fibre bundle in a, the effect by alkaline pectate lyase, effectively degraded flax fiber raw material, Effective softening fibre cell membrane, increase the permeability of fiber, the profit that fully absorbed water beneficial to fiber rises, and lifts the longitudinal direction to flax fiber Split thin, significantly improve fibre fineness, improve fiber quality;
B, in the presence of alkaline pectate lyase, while passing through the effect of appropriate alkaline exocellulase, energy Enough quick and abundant broken fibre primary walls, are conducive to the stripping of outer layer of secondary wall, fiber is obtained part wire-dividing broom purification;It is sharp again Continued to act on the noncrystalline domain of microfibre with alkaline endo cellulase, it is exposed non reducing end, be effectively increased fibre Contact area between dimension, increases the specific surface area of fiber and the quantity of free hydroxyl group, thus in spinning process, fiber is mutual Closely weave in, makes raising be significantly improved into the intensity of paper, effectively reduces fiber fines and is lost in;
C, the effect by alkalescent xylanase, rationally the content of the hemicellulose in control flax fiber raw material is 10% Within~20%, fiber cohesion is reduced so that fiber becomes more soft, and beneficial to the fibrillating of fiber, fibre fineness is entered One step increase so that fiber obtains more longitudinal splitting chances, reduces transversely cutting, effectively protects fiber;Improving fiber can The property spun;
The lignin that d, appropriate laccase are effectively separated after degraded is acted on through above-mentioned enzyme system with flax shive:Collaboration is compound Cellulase and pectase collective effect are in plant cell wall.
Applicant also proposed a kind of flax fiber raw material Degumming method, comprise the following steps:
Step (1):Flax fiber raw material, complex enzyme formulation and water are well mixed in bucking kier;
Step (2):Flax fiber raw material after step (1) enzyme effect is washed and obtained again through hydrogen peroxide bleaching process Obtain flax fiber;Using the high ph-values and the operational characteristic of high temperature of oxygen bleaching, further cull is removed, so as to reach flax The purpose of fibrous raw material degumming.
Wherein, complex enzyme formulation mass percent is the 1 ‰ to 1.5 ‰ of flax fiber raw material;Complex enzyme formulation includes 70 ﹪ alkaline pectate lyase, 2 ﹪ alkali cellulose enzyme, 18 ﹪ alkalescent xylanase and 10% laccase, it is above-mentioned Component ratio is mass percent:
During the enzyme effect of step (1), water circulation is carried out by circulating pump and persistently stirred, holding acts on following bar Part:Temperature maintains 50 DEG C~60 DEG C, and pH value maintains 7.0~8.5, and usually time is 2h~4h;Therebetween, by adding soda ash Adjust pH value and pH value is more than 6.5 after effect terminates.
Using above-mentioned technical proposal, the pectic substance, part half due to complex enzyme direct hydrolysis in flax fiber raw material are fine The class materials such as the plain xylan of dimension, glucan, heteroglycan and the lignin that is separated with fibre bundle, its hydrolysate is soluble in water and through water Efficiently separated after washing with fiber, so that bleaching liquor and amount of caustic soda decline 20%, 40% respectively in the bleaching process of rear end;It is simultaneously raw The color for producing waste water is improved as present light brown or yellow, COD reductions by more than 30% by original black or dark brown;Due to multiple The effect of synthase, it is small on fibre damage in scouring processes, such that the influence fiber breaking strength of fiber spinnability, intensity are even The important indicators such as degree, fineness and elongation at break are significantly improved, so that fiber spinnability is obviously improved, so as to improve warp Ji benefit, reduces production cost;Acted on, be greatly reduced de- by the mutual cooperation between various enzymes in complex enzyme in the present invention The glue time, so as to improve formation efficiency.
Applicant also proposed a kind of preparation method of the complex enzyme formulation for flax fiber raw material degumming, and its feature exists In comprising the following steps:
Step (1):By raw material enzyme preparation alkaline pectate lyase, alkali cellulose enzyme, alkalescent xylanase and laccase Pre-treatment is carried out respectively;
Step (2):It is 70 ﹪ alkaline pectate lyase, 2 ﹪ alkali cellulose enzyme, 18 ﹪ by mass percent Alkalescent xylanase and 10% laccase are well mixed in blender;
Step (3):Then add appropriate preservative and carried out by diatomite filter it is degerming, then by determining after enzyme activity Prepare the complex enzyme formulation for flax fiber raw material degumming.
Embodiment 1
Pilot production is carried out in Zhejiang Jiaxing producer, wherein, complex enzyme formulation specific formula is as follows, mass percent For:The ﹪ of alkaline pectate lyase 70, the ﹪ of alkali cellulose enzyme 2, the ﹪ of alkalescent xylanase 18, laccase 10%.
By raw material enzyme preparation respectively through pre-treatment, then it is well mixed in blender in proportion, appropriate preservative is then added, Degerming, then logical measure enzyme activity, quantitative package, as product is carried out by diatomite filter.
In 30 days-July 29 June in 2015, totally 30 days, 3 tons of flax spun yarns are produced daily.Bucking kier processing 1 per tank Ton raw fibre, enzyme-added 1200 grams, enzyme concentration 1.2 ‰ is combined enzyme effect process conditions:Operative temperature:55℃;PH value:7.5; Action time:2h;Stirring:Circulating pump carries out water circulation;Effect terminates pH value:6.5 more than.Creation data is as shown in table 1 below.
Table 1
Embodiment 2
In Jiangsu Taizhou, producer carries out pilot production, and complex enzyme formulation specific formula is as follows, and mass percent is:Alkalescence The ﹪ of transelminase 70, the ﹪ of alkali cellulose enzyme 2, the ﹪ of alkalescent xylanase 18, laccase 10%.
By raw material enzyme preparation respectively through pre-treatment, then it is well mixed in blender in proportion, appropriate preservative is then added, Degerming, then logical measure enzyme activity, quantitative package, as product is carried out by diatomite filter.
In 7 days~December 2 November in 2015, totally 26 days, 2.5 tons of flax spun yarns are produced daily.At the every tank of bucking kier 1 ton of raw fibre is managed, enzyme-added 1500 grams, enzyme concentration 1.5 ‰ is combined enzyme effect process conditions:Operative temperature:50℃;PH value: 8.0;Action time:3h is stirred:Circulating pump carries out water circulation;Effect terminates pH value:6.8 more than.
Creation data is as shown in table 2 below.
Table 2
The explanation of above example is only intended to the method and its core concept for helping to understand the present invention.It should be pointed out that pair , under the premise without departing from the principles of the invention, can also be to present invention progress for those skilled in the art Some improvement and modification, these are improved and modification is also fallen into the protection domain of the claims in the present invention.
The foregoing description of the disclosed embodiments, enables professional and technical personnel in the field to realize or using the present invention. A variety of modifications to these embodiments will be apparent for those skilled in the art, as defined herein General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, it is of the invention The embodiments shown herein is not intended to be limited to, and is to fit to and principles disclosed herein and features of novelty phase one The most wide scope caused.

Claims (6)

1. a kind of complex enzyme formulation for flax fiber raw material degumming, it is characterised in that including alkaline pectate lyase, alkali Property cellulase, alkalescent xylanase and laccase, weight/mass percentage composition of each component in complex enzyme formulation are as follows:
Alkaline pectate lyase:70 ﹪;
Alkali cellulose enzyme:2 ﹪;
Alkalescent xylanase:18 ﹪;
Laccase:10%;
Also include basic poly galacturonic acid enzyme and alkaline pectin esterase in the alkaline pectate lyase, quality percentage contains Amount is as follows:
Alkaline pectate lyase:70 ﹪;
Basic poly galacturonic acid enzyme:15%;
Alkaline pectin esterase:15%;
The alkali cellulose enzyme includes circumscribed alkali cellulose enzyme, alkaline endo cellulase and alkaline 1,4 beta-glucanase, matter Measure percentage composition as follows:
Circumscribed alkali cellulose enzyme:60 ﹪;
Alkaline endo cellulase:30 ﹪;
Alkaline 1,4 beta-glucanase:10%;
The alkalescent xylanase includes inscribe alkalescent xylanase and circumscribed alkalescent xylanase, and weight/mass percentage composition is as follows:
The ﹪ of inscribe alkalescent xylanase 80;
The circumscribed ﹪ of alkalescent xylanase 20.
2. a kind of flax fiber raw material Degumming method, it is characterised in that comprise the following steps:
Step (1):Flax fiber raw material, complex enzyme formulation and water are well mixed in bucking kier;
Step (2):Flax fiber raw material after step (1) enzyme effect is washed and obtains flax fibre through bleaching process again Dimension;
Wherein, the complex enzyme formulation mass percent is the 1 ‰ to 1.5 ‰ of flax fiber raw material;The complex enzyme formulation bag 70 ﹪ alkaline pectate lyase, 2 ﹪ alkali cellulose enzyme, 18 ﹪ alkalescent xylanase and 10% laccase is included, on It is mass percent to state component ratio:
Also include basic poly galacturonic acid enzyme and alkaline pectin esterase in the alkaline pectate lyase, quality percentage contains Amount is as follows:
Alkaline pectate lyase:70 ﹪;
Basic poly galacturonic acid enzyme:15%;
Alkaline pectin esterase:15%;
The alkali cellulose enzyme includes circumscribed alkali cellulose enzyme, alkaline endo cellulase and alkaline 1,4 beta-glucanase, matter Measure percentage composition as follows:
Circumscribed alkali cellulose enzyme:60 ﹪;
Alkaline endo cellulase:30 ﹪;
Alkaline 1,4 beta-glucanase:10%;
The alkalescent xylanase includes inscribe alkalescent xylanase and circumscribed alkalescent xylanase, and weight/mass percentage composition is as follows:
The ﹪ of inscribe alkalescent xylanase 80;
The circumscribed ﹪ of alkalescent xylanase 20;
During the enzyme effect of step (1), temperature maintains 50 DEG C~60 DEG C, and pH value maintains 7.0~8.5, usually time For 2h~4h.
3. flax fiber raw material Degumming method according to claim 2, it is characterised in that used in the step (2) Hydrogen peroxide bleaching process.
4. flax fiber raw material Degumming method according to claim 2, it is characterised in that in the step (1), is added Soda ash adjusts pH value and pH value is more than 6.5 after effect terminates.
5. flax fiber raw material Degumming method according to claim 2, it is characterised in that in the step (1), pass through Circulating pump carries out water circulation and persistently stirred.
6. the preparation method of a kind of complex enzyme formulation for flax fiber raw material degumming, it is characterised in that comprise the following steps:
Step (1):By raw material enzyme preparation alkaline pectate lyase, alkali cellulose enzyme, alkalescent xylanase and laccase difference Carry out pre-treatment;
Step (2):By alkalescence of the mass percent for 70 ﹪ alkaline pectate lyase, 2 ﹪ alkali cellulose enzyme, 18 ﹪ Zytase and 10% laccase are well mixed in blender;
Also include basic poly galacturonic acid enzyme and alkaline pectin esterase in the alkaline pectate lyase, quality percentage contains Amount is as follows:
Alkaline pectate lyase:70 ﹪;
Basic poly galacturonic acid enzyme:15%;
Alkaline pectin esterase:15%;
The alkali cellulose enzyme includes circumscribed alkali cellulose enzyme, alkaline endo cellulase and alkaline 1,4 beta-glucanase, matter Measure percentage composition as follows:
Circumscribed alkali cellulose enzyme:60 ﹪;
Alkaline endo cellulase:30 ﹪;
Alkaline 1,4 beta-glucanase:10%;
The alkalescent xylanase includes inscribe alkalescent xylanase and circumscribed alkalescent xylanase, and weight/mass percentage composition is as follows:
The ﹪ of inscribe alkalescent xylanase 80;
The circumscribed ﹪ of alkalescent xylanase 20;
Step (3):Then add appropriate preservative and carried out by diatomite filter it is degerming, then by determine after enzyme activity prepare Go out the complex enzyme formulation for flax fiber raw material degumming.
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