CN105483123A - Genetic marker combination, individual gene identity certificate and uses thereof - Google Patents
Genetic marker combination, individual gene identity certificate and uses thereof Download PDFInfo
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Abstract
The invention discloses a genetic marker combination, comprising SNP (single nucleotide polymorphism) sites as shown in table 1. The invention further discloses an individual gene identity certificate and a kit. The genetic marker combination, the individual gene identity certificate and/or the kit are effective in identifying genetic information of individuals and/or distinguishing their identities.
Description
Technical field
The present invention relates to biomedical sector, concrete, the present invention relates to the combination of a kind of genetic marker, a kind of genes of individuals identity card, a kind of test kit and uses thereof.
Background technology
Genetic information entails filial generation by parental generation, and be changeless for individuality in theory, therefore gene test (except the abrupt climatic change of tumour) can be used in discriminate individuals, for certification individual identity information.
Genetic ID card (GeneIdentificationCard) mainly utilizes DNA fingerprint technology, chooses several fixing gene locuss and identifies.Gene is intrinsic constant genetic marker, is inherited, only need choose several sites and just can identify out by child from father and mother there.Corresponding to identification number, the number of genes of individuals identity card can represent that the data of these gene locus features represent by multiple, and the combination of these gene locuss elected has uniqueness.
Summary of the invention
The present invention is intended at least solve the problem one of at least or provides at least one selectable commercial means.
According to an aspect of of the present present invention, the invention provides the combination of a kind of genetic marker, described genetic marker combination comprises the SNP site shown in table 1.
Table 1
| rs9951171 | rs560681 | rs2046361 | rs10488710 |
| rs993934 | rs4606077 | rs1821380 | rs10092491 |
| rs9905977 | rs4530059 | rs1736442 | rs10776839 |
| rs987640 | rs445251 | rs159606 | rs10773760 |
| rs901398 | rs4364205 | rs1523537 | rs1058083 |
| rs891700 | rs430046 | rs1498553 | rs2342747 |
| rs8078417 | rs3780962 | rs1490413 | rs2269355 |
| rs7520386 | rs338882 | rs1336071 | rs221956 |
| rs740598 | rs321198 | rs13218440 | rs6811238 |
| rs722290 | rs2920816 | rs13182883 | rs6444724 |
| rs7041158 | rs279844 | rs1294331 | rs576261 |
| rs6955448 | rs2399332 | rs1109037 |
Single nucleotide polymorphism (singlenucleotidepolymorphism, SNP), mainly refers to the DNA sequence polymorphism caused by the variation of single core thuja acid in genomic level.It is modal one in the heritable variation of the mankind.Account for more than 90% of all known polymorphisms.SNP extensively exists in human genome, on average just has 1 in every 500 ~ 1000 base pairs, estimate its sum can reach 3,000,000 even more.
It should be noted that, the SNP site in above table 1 represents according to the naming method of GenBankSNP database, and which adds numerical digit Arabic numerals to represent a SNP site determined with rs or ac.Those skilled in the art will know that, a SNP site can also have other representation, representing with reference to the position on cDNA as marked certain site with HGVS nomenclature, such as rs1801394 and MTRR gene c.66A>G (A66G) represent same site.Other naming method is adopted such as to refer to SNP or the SNP combination the same with the present invention with this SNP and also belong to the scope of the invention marking with reference to the position on gDNA.
The determination of alleged genetic marker combination comprises contriver and screens based on the SNP higher to occurrence frequency in human genome, and be combined into one for individual be unique combination, thus carry out corresponding with individual information, the information of genetic marker combination can as " genetic ID card " of individuality.SNP site invention shown in table 1 is through screening combination and determine through verification experimental verification, can effectively combine for the genetic marker of idiogenetics data separation.
According to embodiments of the invention, contriver obtains the SNP site of table 1 by design following methods: first, all genetic marker information disclosing races different more than 50 in retrievable human race's genomic information are carried out arrange, statistical study and sequence, average heterozygosity (Averageheterozygosity) is greater than 0.4, SNP that fixation index (TheFixationindex, FST) is less than 0.06 filters out; Then, consider each SNP position on chromosome filtered out, Haplotype distribution etc., filter out the site of distance more than 75cM of adjacent two SNP on Single chromosome.The genetic marker combination that this method is screened overcomes traditional combines the low problem of crowd's resolving power that may occur based on STR, and can the detection platform of Selection utilization many, be beneficial to and detect when extremely low initiate dna.
According to embodiments of the invention, this genetic marker combination on the one hand of the present invention can also have following additional technical feature one of at least:
With the gene of phenotypic correlation, mainly with the color of eyes, skin, hair, and the gene of shape of face, the many gene of current research comprises: TYR, TYRP1, OCA2, SLC45A2, SLC24A5, MC1R, ASIP, KITLG, SLC24A4, IRF4, TPCN2 etc., the pleomorphism site on these genes can predict the Relevant phenotype of this tester.Regrettably, the research about Asian shape of face and phenotypic correlation is less.According to embodiments of the invention, contriver considers that the information with the SNP site in the supplementary of the SNP in table 1 and his-and-hers watches 1 distinguishes the enhancing of effect as individuality, screen the SNP site of some and Aisa people's phenotypic correlation as main candidate, make at least one that the combination of described genetic marker also comprises in following SNP site: rs17822931, rs1800414, rs1800419, rs1805008, rs671, rs3827760, rs11671664 and rs11030104.Such as, genetic marker combination also comprise in listed 8 any one, two, three, five or preferably comprise whole eight.
These 8 SNP site are that contriver screens and the individual information of the SNP site combination of enhancing table 1 further can distinguish the site of Aisa people's phenotypic correlation of function through verification experimental verification.Such as, rs17822931 is relevant to earwax, rs1800414 is relevant to the Asian light colour of skin, rs1800419 is relevant to skin and eye color, rs1805008 is relevant to skin color, rs671 to drink blush relevant, rs3827760 and straight hair, thick and spade tooth relevant, rs11671664 is relevant to the heavy body weight of Aisa people, and rs11030104 increases relevant to Aisa people's body weight.So, this genetic marker is combined and is more suitable for distinguishing with the crowd of the artificial central genetic background in Asia, not only increase the accuracy that individuality distinguished of tradition based on STR mark, add the accuracy distinguishing difficult sample simultaneously.
The information combined for making genetic marker has the resolution function of better individual affiliated race, and contriver combines the site information and composite test checking that comprise in conjunction with genetic marker, filtered out the site shown in table 2.
According to embodiments of the invention, the combination of described genetic marker also comprise in the SNP site shown in table 3 at least one.Such as, the combination of this genetic marker also comprise in SNP listed by table 3 any one, two, three, five, ten, 20,30,40,50 or preferably comprise the whole SNP site shown in table 2.That table 3 illustrates resolution function that contriver filters out, that can strengthen original genetic marker combined information, that race can be distinguished SNP site combination.
Table 2
| rs2814778 | rs1919550 | rs6990312 | rs4411548 | rs2593595 |
| rs3737576 | rs1572018 | rs3814134 | rs4471745 | rs4833103 |
| rs7554936 | rs2166624 | rs4918664 | rs2042762 | rs7657799 |
| rs10497191 | rs7326934 | rs1079597 | rs3916235 | rs3823159 |
| rs1834619 | rs7997709 | rs174570 | rs7722456 | rs917115 |
| rs1876482 | rs9522149 | rs2238151 | rs870347 | rs1462906 |
| rs260690 | rs200354 | rs4891825 | rs16891982 | rs12913832 |
| rs3827760 | rs12439433 | rs7226659 | rs192655 | rs459920 |
| rs6754311 | rs1426654 | rs7251928 | rs1871534 | rs11652805 |
| rs798443 | rs1800414 | rs310644 | rs2196051 | rs1229984 |
| rs12498138 | rs735480 | rs2024566 | rs17642714 | rs3811801 |
The SNP site relevant to blood lineage is generally from two aspects: the mitochondrial inheritance of mother and the Y chromosome heredity from father, these two independently genetic system can represent blood lineage or family.The information combined for making genetic marker has the resolution function in better individual blood lineage or family source, and contriver combines the site information and composite test checking that have comprised in conjunction with genetic marker, filtered out the site shown in table 3.
According to embodiments of the invention, the combination of described genetic marker also comprise in the SNP site shown in table 3 at least one.Such as, genetic marker combination also comprise in site listed by table 3 any one, two, three, five, ten, 20,30,40,50 or preferably comprise the whole SNP site shown in table 3.Pleomorphism site on the Y chromosome of blood lineage's information that resolution function that contriver filters out, that can strengthen original genetic marker combined information shown by table 3, that better strengthen marker combination.
Table 3
| Rs3893 | Rs2032665 | AC010889 | AC009977 |
| Rs3900 | AC010889 | AC010889 | AC010889 |
| Rs3908 | Rs2534636 | AC009977 | Rs2032640 |
| Rs3909 | AC005820 | AC006040 | Rs2032652 |
| Rs3010 | Rs2535813 | Rs2032597 | AC010889 |
| AC009977 | Rs150173 | Rs2032604 | AC010889 |
| AC009977 | AC010889 | Rs2032624 | AC010137 |
| Rs1179188 | AC010137 | Rs2032678 | Rs2032631 |
| AC006040 | AC002531 | Rs2032664 |
The information combined for making genetic marker has the resolution function in better individual blood lineage or family source, and contriver combines the site information and composite test checking that have comprised in conjunction with genetic marker, filtered out the pleomorphism site on plastosome further.
According to embodiments of the invention, the combination of described genetic marker also comprises following plastosome site one of at least: 152,709,3010,3970,5178,8414,9bp, 9540,10398,10873,12705,13928,14783,15043,16311 and 16362; 9bp site wherein refers to the base CCCCCTCTA with described mitochondrial 8272nd these mitochondrial 8281 ~ 8289 positions of repeating to 9 bases on the 8280th, and described 9bp site exists the base CCCCCTCTA entirety disappearance that sudden change shows as described mitochondrial 8281 ~ 8289 positions.Such as, genetic marker combination also comprise in above-mentioned listed plastosome site any one, two, three, five, ten, 15 or preferably comprise all listed plastosome sites.It should be noted that, here this site is represented at plastosome with reference to the Position Number on genome with site, such as plastosome 152 site refers to C genotype on the 152nd on plastosome reference sequences and other possible genotype, G genotype on the 709th on 709 finger plastosome reference sequences and other possible genotype, G genotype on the 3010th on 3010 finger plastosome reference sequences and other possible genotype, T genotype on the 3970th on 3970 finger plastosome reference sequences and other possible genotype, A genotype on the 5178th on 5178 finger plastosome reference sequences and other possible genotype, T genotype on the 8414th on 8414 finger plastosome reference sequences and other possible genotype, T genotype on the 9540th on 9540 finger plastosome reference sequences and other possible genotype, C genotype on the 10398th on 10398 finger plastosome reference sequences and other possible genotype, C genotype on the 10873rd on 10873 finger plastosome reference sequences and other possible genotype, T genotype on the 12705th on 12705 finger plastosome reference sequences and other possible genotype, C genotype on the 13928th on 13928 finger plastosome reference sequences and other possible genotype, T genotype on the 14783rd on 14783 finger plastosome reference sequences and other possible genotype, C genotype on the 15043rd on 15043 finger plastosome reference sequences and other possible genotype, A genotype on the 16311st on 16311 finger plastosome reference sequences and other possible genotype, G genotype on the 16362nd on 16362 finger plastosome reference sequences and other possible genotype.
The information combined for making genetic marker has stronger individual information uniqueness and individual identity differentiates function, and contriver combines the site information and composite test checking that have comprised in conjunction with genetic marker, screening determines one group of STR site further.
According to embodiments of the invention, the combination of described genetic marker also comprises following STR site one of at least: D3S1358, vWA, D16S539, CSF1PO, TPOX, Yindel, D8S1179, D21S11, D18S51, DYS391, D2S441, D19S433, TH01, FGA, D22S1045, D5S818, D13S317, D7S820, SE33, D10S1248, D1S1656, D12S391, D2S1338, PENTAE and PENTA.Such as, genetic marker combination also comprise in listed 25 STR sites any one, two, three, five, ten, 15,20 or preferably comprise whole 25 STR sites.
According to another aspect of the present invention, the invention provides a kind of genes of individuals identity card, described genes of individuals identity card comprises chip structure, described chip carries the genotype information in the multiple genetic markers site of described individuality, described multiple genetic markers site comprises the site in the genetic marker combination of the invention described above on the one hand or in arbitrary embodiment.Above-mentioned to the technical characteristic of genetic marker combination in one aspect of the present invention or any embodiment and the description of advantage, be suitable for the genes of individuals identity card of this one side of the present invention equally, do not repeat them here.
The genes of individuals identity card of this one side of the present invention, combines the type information in the site in the genetic marker combination determined based on above-mentioned arbitrary contriver's screening verification, can effectively easily as individual identity certification foundation.Alleged individuality is preferably Aisa people.Alleged individual identity information, includes but not limited to individual phenotype, affiliated race, affiliated blood lineage's family information etc.
Genetic typing also claims gene type, is the powerful carrying out Genetic identification such as paternity test, criminal's identification, Missing Persons's identification.Genetic evidence can get from any biologic material, includes but not limited to hair, blood, sperm, bone, tooth, muscle and saliva.Medical law fields is used for having have passed through progress for several times, from mark itself to used method to the genetic marker storehouse that biomaterial is classified.Loci carries out somatotype, and can utilize currently known methods, the present invention is not restricted this.Such as carry out somatotype to SNP site, utilizable method includes but not limited to direct sequencing, fragment length polymorphism (restriction enzyme) method, fluorescently-labeled fragment length polymorphism method, flight mass spectrum detection method, Tagman fluorescence probe method, multiple SNaPShot detection method, LDR Ligase detection reaction method, the ligase enzyme detection method (iMLDR) of improvement, gene chips, SNPSCAN typing, high resolving power melting curve method and the SNP classifying method based on qPCR method.According to one embodiment of present invention, utilize high-flux sequence analytical procedure to carry out somatotype to genetic marker site, obtain the genotype in multiple site simultaneously.
The structural style of the present invention to genes of individuals identity card is not restricted.Can be such as the card form structure as current personal identity card, it be embedded with chip can read genetic marker site type information for machine, can be also the structures such as similar hard disk, CD, facilitate instrument reading displayed information.
According to another aspect of the invention, provide a kind of test kit, described test kit comprises gene type reagent, and described gene type reagent can be used in the somatotype in the site of the invention described above on the one hand or in the genetic marker combination of any embodiment.Above-mentioned to the technical characteristic of genetic marker combination in one aspect of the present invention or any embodiment and the description of advantage, be suitable for the genotyping kit of this one side of the present invention equally, do not repeat them here.
Carry out somatotype to SNP site, utilizable method includes but not limited to direct sequencing, fragment length polymorphism (restriction enzyme) method, fluorescently-labeled fragment length polymorphism method, flight mass spectrum detection method, Tagman fluorescence probe method, multiple SNaPShot detection method, LDR Ligase detection reaction method, the ligase enzyme detection method (iMLDR) of improvement, gene chips, SNPSCAN typing, high resolving power melting curve method and the SNP classifying method based on qPCR method.According to one embodiment of present invention, this test kit comprises high-flux sequence reagent, comprise library construction reagent, sequencing reagent etc., utilize this test kit can realize the somatotype utilizing high-flux sequence analytical procedure to genetic marker site, obtain the genotype in multiple site simultaneously.
According to another aspect of the invention, the invention provides the genetic marker combination of a kind of the invention described above one side or any embodiment, genes of individuals identity card or the purposes of test kit in qualification idiogenetics information and/or discriminate individuals identity.Alleged individuality is preferably Aisa people.Above-mentioned to the genetic marker combination in one aspect of the present invention or any embodiment, genes of individuals identity card and the technical characteristic of test kit and the description of advantage, be suitable for the purposes of this one side of the present invention equally, do not repeat them here.
Embodiment
Embodiment is that the present invention is described in detail below, and embodiment described below is exemplary, only for explaining the present invention, and can not be interpreted as limitation of the present invention.
" reference sequences " in the present invention for known group sequence or known group sequence at least partially.In describing the invention, except as otherwise noted, the implication of " multiple " is two or more.
In addition, the present invention can in different example repeat reference numerals and/or reference letter, this repetition is to simplify and clearly object, itself does not indicate the relation between discussed various embodiment and/or setting.
Below except as otherwise explaining, the reagent do not explained especially related in following examples, sequence (joint, label and primer), software and instrument, be all conventional commercial product or increase income, such as purchased from Illumina, LifeTechnologies company etc.
Embodiment 1
Obtain the genetic marker combination of candidate by the following method.
Obtain the SNP site of table 1: first, all genetic marker information disclosing races different more than 50 in retrievable human race's genomic information are carried out arrange, statistical study and sequence, comprise various public data storehouse, as NCBI, EBI etc., average heterozygosity (Averageheterozygosity) is greater than 0.4, fixation index (TheFixationindex, FST) SNP being less than 0.06 filters out, and average heterozygosity and fixation index can obtain from disclosed database; Then, consider each SNP position on chromosome filtered out, Haplotype distribution etc., filter out the site of distance more than 75cM of adjacent two SNP on Single chromosome.
The SNP of the table 1 of such acquisition with the polymorphic position point reflection individual information of a small amount of high heterozygosis, can differentiate individuality for distinguishing.And the genetic marker combination of screening of this method overcomes and traditional combines the low problem of crowd's resolution that may occur based on STR, and can the detection platform of Selection utilization many, be beneficial to and detect when extremely low initiate dna.
Obtain idiogenetics material, utilize high-flux sequence analysis to carry out somatotype to all sites of the table 1 of individuality, the type information in each site is stored on biochip, obtains the genetic ID card of this individuality.
Embodiment 2
Making the genetic marker in embodiment 1 combine the representative genetic marker site comprising more individual phenotype by screening and test combinations checking, making to be more suitable for for distinguishing asian population.
The site comprised into Aisa people's phenotypic correlation of genetic marker combination is as follows: rs17822931 earwax is correlated with, the Asian light colour of skin of rs1800414 is correlated with, rs1800419 skin is relevant with eye color, rs1805008 skin color is correlated with, rs671 drink blush relevant, rs3827760 straight hair, thick, spade tooth is correlated with, rs11671664 Aisa people's body weight is correlated with, and rs11030104 Aisa people's body weight increases relevant.
Make the card that can be used for genes of individuals authentication of the genotype information comprising all sites in individual genetic marker combination.
Embodiment 3
By screening and test combinations checking the genetic marker in embodiment 2 is combined comprise more race relevant, genetic marker site that family is relevant, make to be more suitable for for distinguishing asian population.
The race site of being correlated with comprised into genetic marker combination is as shown in table 2.
The blood lineage Y chromosome site of being correlated with comprised into genetic marker combination is as shown in table 3.
The plastosome site comprised into genetic marker combination is selected from: 152,709,3010,3970,5178,8414,9bp, 9540,10398,10873,12705,13928,14783,15043,16311 and 16362; 9bp site wherein refers to the base CCCCCTCTA with described mitochondrial 8272nd these mitochondrial 8281 ~ 8289 positions of repeating to 9 bases on the 8280th, and described 9bp site exists the base CCCCCTCTA entirety disappearance that sudden change shows as described mitochondrial 8281 ~ 8289 positions.Here this site is referred at plastosome with reference to the position on genome with site, C genotype on the 152nd on 152 finger plastosome reference sequences wherein and other possible genotype, G genotype on the 709th on 709 finger plastosome reference sequences and other possible genotype, G genotype on the 3010th on 3010 finger plastosome reference sequences and other possible genotype, T genotype on the 3970th on 3970 finger plastosome reference sequences and other possible genotype, A genotype on the 5178th on 5178 finger plastosome reference sequences and other possible genotype, T genotype on the 8414th on 8414 finger plastosome reference sequences and other possible genotype, T genotype on the 9540th on 9540 finger plastosome reference sequences and other possible genotype, C genotype on the 10398th on 10398 finger plastosome reference sequences and other possible genotype, C genotype on the 10873rd on 10873 finger plastosome reference sequences and other possible genotype, T genotype on the 12705th on 12705 finger plastosome reference sequences and other possible genotype, C genotype on the 13928th on 13928 finger plastosome reference sequences and other possible genotype, T genotype on the 14783rd on 14783 finger plastosome reference sequences and other possible genotype, C genotype on the 15043rd on 15043 finger plastosome reference sequences and other possible genotype, A genotype on the 16311st on 16311 finger plastosome reference sequences and other possible genotype, G genotype on the 16362nd on 16362 finger plastosome reference sequences and other possible genotype.
The STR site comprised into genetic marker combination is selected from following: D3S1358, vWA, D16S539, CSF1PO, TPOX, Yindel, D8S1179, D21S11, D18S51, DYS391, D2S441, D19S433, TH01, FGA, D22S1045, D5S818, D13S317, D7S820, SE33, D10S1248, D1S1656, D12S391, D2S1338, PENTAE and PENTA.
Somatotype is carried out to all sites in the genetic marker combination of individuality, makes the card of the genotype information of all sites comprised in individual combination.
Embodiment 4
Utilize the genotype detection to the site that the genetic marker of embodiment 1-3 combines, the kinsfolk that there is kinship for 20 carries out random sample experiment and interpretation of result.
1, individual specimen
We amount to 65 mankind's blood DNA samples to the kinsfolk of 20 kinships comprising child father and mother, brother, sister, cousin etc. and test, and concrete scheme is as follows.
2, library construction and order-checking
The blood DNA of 65 people is carried out random number and carries out double blind experiment by us, proceeds as follows respectively:
2.1 sample extraction
The DNA extraction reagent used, extracts blood DNA.Key step is as follows: be temporary in by whole blood sample trees in 4 DEG C of ice feather cockscombs.Adopt the blood DNA of QIAgene company of the U.S. to extract test kit, process repeats no more.Quantitative analysis is carried out to the DNA extracted, ensures that the DNA of each extraction is more than 200ng.Sample is numbered, from No. 1 to No. 65
2.2PCR amplification
DNTPSolutionSet (10mM), MgSO4 (50mM), PCRPrimer (10Pmol/uL) is taken out, sample in advance from-20 DEG C of reagent discs preserved.Be placed in room-temperature dissolution on centrifuge tube shelf, fully of short duration centrifugal after mixing; In the centrifuge tube of 1.5mL, preparation reaction mix, short even rear of short duration centrifugal, packing; After MiX divides and installs, of short duration centrifugal after mixing.PCR reaction system is as follows:
| DNA | 32.2μL |
| Platinum Pfx DNA Ploymerase | 0.8μL |
| Primer | 4μL |
| MgSO4(50mM) | 2μL |
| dNTP Solution Set | 2μL |
| 10X Pfx buffer | 5ul |
| ddH2O | 4ul |
| Total volume | 50μL |
PCR response procedures is:
After having reacted, carry out purifying with paramagnetic particle method, dissolve in 50ul.
2.3 mixing
Different PCR primer is carried out quantitatively, and gets identical product amount and mix, form sequencing library
Agilent2100DNA1000assay.kit3:3 is used to detect library fragments size and concentration, simultaneously quantitative with QPCR.
DNA sequencing can be carried out in fixed measured library.
2.4Hiseq order-checking
Get the order-checking of 10pmolDNA IlluminaHiseqPE-150 program, concrete operations flow process refers to Hiseq process specifications.
3, interpretation of result
The sequencing result of IlluminaHiseq output is a series of DNA sequence dnas, these sequencing sequences can be corresponded to every increment originally by the ID label of sequencing library label, sample, the sequence of same sample is analyzed according to the flow process of paternity test, finally obtains site genotyping result.
Result shows, and carries out the individual family relational result that sample clustering obtains at random, the expection of sampling before meeting.
In the description of this specification sheets, specific features, material or feature that the description of reference term " embodiment ", " some embodiments ", " example ", " concrete example " or " some examples " etc. means to describe in conjunction with this embodiment or example are contained at least one embodiment of the present invention or example.In this manual, identical embodiment or example are not necessarily referred to the schematic representation of above-mentioned term.And the specific features of description, structure, material or feature can combine in an appropriate manner in any one or more embodiment or example.
Although illustrate and describe embodiments of the invention, those having ordinary skill in the art will appreciate that: can carry out multiple change, amendment, replacement and modification to these embodiments when not departing from principle of the present invention and aim, scope of the present invention is by claim and equivalents thereof.
Claims (10)
1. a genetic marker combination, is characterized in that, described genetic marker combination comprises the SNP site shown in table 1.
2. the genetic marker combination of claim 1, is characterized in that, described genetic marker combination also comprises at least one SNP site following:
Rs17822931, rs1800414, rs1800419, rs1805008, rs671, rs3827760, rs11671664 and rs11030104;
Optional, described genetic marker combination also comprises following SNP site:
Rs17822931, rs1800414, rs1800419, rs1805008, rs671, rs3827760, rs11671664 and rs11030104.
3. the genetic marker combination of claim 1, is characterized in that, the combination of described genetic marker also comprise in the SNP site shown in table 2 at least one;
Optional, described genetic marker combination also comprises the SNP site shown in table 2.
4. claim 1-3 arbitrary genetic marker combination, is characterized in that, described genetic marker combination also comprise in the SNP site shown in table 3 at least one;
Optional, described genetic marker combination also comprises the SNP site shown in table 3.
5. the genetic marker combination of claim 4, is characterized in that, the combination of described genetic marker also comprises following plastosome site one of at least:
152,709,3010,3970,5178,8414,9bp, 9540,10398,10873,12705,13928,14783,15043,16311 and 16362, wherein,
Described 9bp site refers to the base CCCCCTCTA of described mitochondrial 8281 ~ 8289 positions of repeating with 9 bases of described plastosome 8272 ~ 8280 position, and described 9bp site exists the base CCCCCTCTA entirety disappearance that sudden change shows as described mitochondrial 8281 ~ 8289 positions;
Optional, described genetic marker combination also comprises following plastosome site:
152,709,3010,3970,5178,8414,9bp, 9540,10398,10873,12705,13928,14783,15043,16311 and 16362, wherein,
Described 9bp site refers to the base CCCCCTCTA of described mitochondrial 8281 ~ 8289 positions of repeating with 9 bases of described plastosome 8272 ~ 8280 position, and described 9bp site exists the base CCCCCTCTA entirety disappearance that sudden change shows as described mitochondrial 8281 ~ 8289 positions.
6. the genetic marker combination of claim 4, is characterized in that, the combination of described genetic marker also comprises following STR site one of at least:
D3S1358, vWA, D16S539, CSF1PO, TPOX, Yindel, D8S1179, D21S11, D18S51, DYS391, D2S441, D19S433, TH01, FGA, D22S1045, D5S818, D13S317, D7S820, SE33, D10S1248, D1S1656, D12S391, D2S1338, PENTAE and PENTA;
Optional, described genetic marker combination also comprises following STR site:
D3S1358, vWA, D16S539, CSF1PO, TPOX, Yindel, D8S1179, D21S11, D18S51, DYS391, D2S441, D19S433, TH01, FGA, D22S1045, D5S818, D13S317, D7S820, SE33, D10S1248, D1S1656, D12S391, D2S1338, PENTAE and PENTA.
7. a genes of individuals identity card, it is characterized in that, described genes of individuals identity card comprises chip structure, described chip carries the genotype information in the multiple genetic markers site of described individuality, and described multiple genetic markers site comprises the site in the arbitrary genetic marker combination of claim 1-6.
8. a test kit, is characterized in that, described test kit comprises gene type reagent, and described gene type reagent can be used in the somatotype in the site in the arbitrary genetic marker combination of claim 1-6.
9. the combination of claim 1-6 arbitrary genetic marker or the genes of individuals identity card of claim 7 or the purposes of the test kit of claim 8 in qualification idiogenetics information and/or discriminate individuals identity.
10. the purposes of claim 9, is characterized in that, described individuality is Aisa people.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105925693A (en) * | 2016-05-13 | 2016-09-07 | 深圳市核子基因科技有限公司 | Genetic identity card and preparation method thereof |
| CN106520982A (en) * | 2016-12-05 | 2017-03-22 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Compound typing system used for personal identification |
| CN106702010A (en) * | 2017-03-06 | 2017-05-24 | 黄捷 | Genetic marker combination, individual gene identity card, two-dimensional code, kit and application thereof |
| CN107012226A (en) * | 2017-04-20 | 2017-08-04 | 司法部司法鉴定科学技术研究所 | A kind of detection kit and its detection method of the SNP site based on high-flux sequence |
| CN107385064A (en) * | 2017-08-16 | 2017-11-24 | 广东华美众源生物科技有限公司 | Fluorescence labeling composite amplification kit that is a kind of while expanding huamn autosomal SNP and STR bit point and its application |
| CN108342489A (en) * | 2017-01-23 | 2018-07-31 | 公安部物证鉴定中心 | A kind of method and system carrying out Y-SNP partings to male individual |
| CN109609660A (en) * | 2018-12-24 | 2019-04-12 | 郑州华之源医学检验实验室有限公司 | A kind of individual identification system, detection method and its application |
| CN110129457A (en) * | 2019-06-19 | 2019-08-16 | 上海仁东医学检验所有限公司 | A kind of combination of genetic marker and its application |
| CN110863056A (en) * | 2018-08-27 | 2020-03-06 | 深圳华大法医科技有限公司 | Method, reagent and application for accurately typing human DNA |
| CN112592981A (en) * | 2020-12-01 | 2021-04-02 | 广州精科医学检验所有限公司 | Primer group, kit and method for DNA archive construction |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105925693A (en) * | 2016-05-13 | 2016-09-07 | 深圳市核子基因科技有限公司 | Genetic identity card and preparation method thereof |
| CN106520982A (en) * | 2016-12-05 | 2017-03-22 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Compound typing system used for personal identification |
| CN106520982B (en) * | 2016-12-05 | 2019-11-08 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | A kind of compound classification system for identity authentication |
| CN108342489A (en) * | 2017-01-23 | 2018-07-31 | 公安部物证鉴定中心 | A kind of method and system carrying out Y-SNP partings to male individual |
| CN106702010A (en) * | 2017-03-06 | 2017-05-24 | 黄捷 | Genetic marker combination, individual gene identity card, two-dimensional code, kit and application thereof |
| CN106702010B (en) * | 2017-03-06 | 2020-07-28 | 黄捷 | Genetic marker combination, individual gene identity card, two-dimensional code, kit and application thereof |
| CN107012226A (en) * | 2017-04-20 | 2017-08-04 | 司法部司法鉴定科学技术研究所 | A kind of detection kit and its detection method of the SNP site based on high-flux sequence |
| CN107385064A (en) * | 2017-08-16 | 2017-11-24 | 广东华美众源生物科技有限公司 | Fluorescence labeling composite amplification kit that is a kind of while expanding huamn autosomal SNP and STR bit point and its application |
| CN107385064B (en) * | 2017-08-16 | 2020-12-15 | 广东华美众源生物科技有限公司 | Fluorescence labeling composite amplification kit for simultaneously amplifying human autosomal SNP and STR loci and application thereof |
| CN110863056A (en) * | 2018-08-27 | 2020-03-06 | 深圳华大法医科技有限公司 | Method, reagent and application for accurately typing human DNA |
| CN109609660A (en) * | 2018-12-24 | 2019-04-12 | 郑州华之源医学检验实验室有限公司 | A kind of individual identification system, detection method and its application |
| CN110129457A (en) * | 2019-06-19 | 2019-08-16 | 上海仁东医学检验所有限公司 | A kind of combination of genetic marker and its application |
| CN112592981A (en) * | 2020-12-01 | 2021-04-02 | 广州精科医学检验所有限公司 | Primer group, kit and method for DNA archive construction |
| CN112592981B (en) * | 2020-12-01 | 2023-06-20 | 广州精科医学检验所有限公司 | Primer group, kit and method for DNA archive construction |
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