CN105482953B - A kind of production technology of selenium-rich blueberry mixed health wine - Google Patents

A kind of production technology of selenium-rich blueberry mixed health wine Download PDF

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CN105482953B
CN105482953B CN201610050617.3A CN201610050617A CN105482953B CN 105482953 B CN105482953 B CN 105482953B CN 201610050617 A CN201610050617 A CN 201610050617A CN 105482953 B CN105482953 B CN 105482953B
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blueberry
selenium
fermentation
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CN105482953A (en
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张顺涛
李春丽
贺丽娜
李艳
于�玲
史楠
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00—Preparation of other alcoholic beverages
    • C12G3/02—Preparation of other alcoholic beverages by fermentation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/236—Ligusticum (licorice-root)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/254—Acanthopanax or Eleutherococcus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/284—Atractylodes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/29—Berberidaceae (Barberry family), e.g. barberry, cohosh or mayapple
    • A61K36/296—Epimedium
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/41—Crassulaceae (Stonecrop family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/45—Ericaceae or Vacciniaceae (Heath or Blueberry family), e.g. blueberry, cranberry or bilberry
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484—Glycyrrhiza (licorice)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/69—Polygalaceae (Milkwort family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
    • A61K36/704—Polygonum, e.g. knotweed
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/80—Scrophulariaceae (Figwort family)
    • A61K36/804—Rehmannia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
    • A61K36/815—Lycium (desert-thorn)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/898—Orchidaceae (Orchid family)
    • A61K36/8984—Dendrobium

Abstract

The invention belongs to health liquor processing technique field, and in particular to a kind of production technology of selenium-rich blueberry mixed health wine.The production technology of the selenium-rich blueberry mixed health wine of the present invention, including following steps：（1）Actication of culture；（2）By step（1）In saccharomyces cerevisiae prepare zymotic fluid；（3）Fermentation：Selenium-rich is inoculated into step（2）Zymotic fluid in；（4）Filtering, separate saccharomyces cerevisiae；（5）Blueberry and Chinese herbal medicine are pre-processed, obtain fermentation medium, then using step（4）In yeast-inoculated it is above-mentioned culture medium fermentation, supersonic wave wall breaking, filtering, ageing, allotment, membrane filtration, sterile filling, obtain selenium-rich blueberry mixed health wine.The present invention is using blueberry as prescription basis, compatibility is a variety of to have anti-oxidant, senile-resistant efficacy Chinese herbal medicine, organic selenium content in the health liquor being prepared, nutritious, instant edible, the characteristic Chinese herbal health care wine of unique flavor, meets demand of the people to health-care food.

Description

A kind of production technology of selenium-rich blueberry mixed health wine
Technical field
The invention belongs to health liquor processing technique field, and in particular to a kind of production technology of selenium-rich blueberry mixed health wine.
Background technology
Selenium is to maintain one of biogenic element of human normal, and selenium deficiency can cause the generation of a variety of diseases.My god Right Selenium in Food is generally relatively low, but the selenium in natural food can not meet the normal need of people, the low water of Food Selenium Flat intake has potential threat to health, it is ensured that and health, preventing and treating have related disorders, should just have sufficient selenium to take in, Be even more important by absorbing enough selenium to prevent to there are related disorders to seem, and inorganic selenium toxicity is higher can not be by mankind institute Utilize, it is the focus studied at present that inorganic selenium is changed into Organic Selenium by plant and microorganism, but the Se content in plant It is universal relatively low, thus can provide high yield inexpensive organic selenium product for the mankind by microorganism conversion.Selenium-enriched food is developed Through as the focus in health food development.
In recent years, with the increasingly raising of living standard, the health perception of people is more and more stronger, the consumption of health-product market And purchasing power also constantly increases, particularly herbal health care product contains extremely abundant nutritional ingredient, not only wherein amino Sour A wide selection of colours and designs, it is deep to be favored by consumer rich in the various trace elements such as potassium, calcium, and vitamin.Fermented type health liquor is not But containing, to the active nutrient component of the great health-care efficacy of human body, and being produced during the fermentation substantial amounts of in vegetable Chinese herbal medicine Various nutritional ingredients（Such as sugar, protein, essential amino acid）, appropriateness, which is drunk, can play preferable health-care effect, As herbal health care field of food or even the product of whole field of health care food most development prospect.
Confirmed at present through scientific research, containing containing abundant nutritive and health protection components, especially anthocyanidin in Blueberry Amount is far above other vegetables and fruit.Often edible blueberry fruit product, can significantly strengthen eyesight, anti-eye strain, battalion Skin is supported, delays cranial nerve aging, has therapeutic action to the disease of capillaries as caused by diabetes, strengthen cardiac function, be pre- Anti- senile dementia etc..American-European scientific research personnel conducted extensive research to the function of blueberries in recent years, have developed many indigo plants Certain kind of berries fruit food, health products and medicine.
CN103602553B discloses a kind of Compound fermentation blueberry health-care wine and its production method, the invention is from blue The certain kind of berries, blueberry leaf, matrimony vine, wheat bran are that raw material produces Composite fermentation type health liquor by composite fermentation production technology, wherein described multiple Closing each component proportion in zymotic fluid is：Blueberry leaf extract 17~23%, blueberry juice 6~10%, wolfberry juice 10~ 15%th, wheat bran saccharified liquid 55~65%.The product nutritive value is high, keeps the natural nutrient component in blueberry, blueberry leaf substantially, And the nutriments such as the anthocyanidin of needed by human body, vitamin are rich in, there is enhancing eyesight, anti-eye strain, skin nutrition, profit Lung, strengthening spleen and nourishing stomach, delay cranial nerve aging, prevention disease of capillaries, strengthen cardiac function, prevent senile dementia, be antifatigue And other effects.The invention has the advantages that：When using the technique of ultrasonic assistant extraction, and acted on by microbial fermentation, activity Material recovery rate is high；Second, using blueberry leaf, blueberry, matrimony vine and wheat bran composite fermentation, the nutritive water of blueberry mixed health wine is improved It is flat；Third, using the technique of micro-pore-film filtration, product clarity is high, degerming, good impurity removing effect, instead of traditional pasteurization Method, nutrient component damages are small in product.
The content of the invention
In order to solve above-mentioned technical problem, the invention provides one kind using blueberry as primary raw material, it is aided with plurality of Chinese Component such as matrimony vine, wilsonii, Ligusticum wallichii, barrenwort, bighead atractylodes rhizome etc., synergy, anti-oxidant, anti-aging are played jointly, improves body The effect of immunity, and the health liquor of the present invention is turned inorganic selenium using the two-step fermentation zymotechnique of high activity Se-enriched yeast Security and the more preferable Organic Selenium of auxotype are turned to, improves the organic selenium content in health liquor.
The production technology of the selenium-rich blueberry mixed health wine of the present invention, including following steps：
（1）Actication of culture：Activate selenium-rich saccharomyces cerevisiae；
（2）Zymotic fluid is prepared：Saccharomyces cerevisiae one-step fermentation culture medium is prepared, obtains zymotic fluid；
（3）Fermentation：By step（1）Selenium-rich saccharomyces cerevisiae after middle activation is inoculated into step（2）Zymotic fluid in；
（4）Filtered after fermentation ends, separate saccharomyces cerevisiae；
（5）Prepare mixed solution；
Prepare each raw material by following portions by weight：
40~50 parts of blueberry, 20~30 parts of matrimony vine, 15~20 parts of wilsonii, 12~15 parts of Ligusticum wallichii, 8~10 parts of barrenwort, It is 8~10 parts of the bighead atractylodes rhizome, 6~8 parts of the dried rhizome of rehmannia, 4~6 parts of the stem of noble dendrobium, 3~5 parts of polygala, 5~10 parts of the fleece-flower root, 15~20 parts of rhodiola root, sweet Careless 5~10 parts, 10~15 parts of STEVIA REBAUDIANA；
Blueberry pre-processes：Blueberry is taken, adds water, is soaked, mashing, obtains blueberry pulp；
Chinese herbal medicine pre-processes：Herbal component is taken, is mixed after crushing, pure water is added, then adds cellulase, super Extracted under sound wave subsidiary conditions, obtain herbal liquid medicine；
（6）By step（5）In obtain blueberry pulp and mixed with herbal liquid medicine, added in obtained mixed solution inorganic Salt, obtain Chinese herbal medicine fermentation medium；
（7）By step（4）Saccharomyces cerevisiae after filtering is inoculated into Chinese herbal medicine fermentation medium by 10% inoculum concentration；
（8）To step（7）In obtained culture medium fermentation, supersonic wave wall breaking, filtering, ageing, allotment, membrane filtration, sterile It is filling, obtain selenium-rich blueberry mixed health wine.
Preferably, above-mentioned step（1）In, the high activity selenium-rich saccharomyces cerevisiae of preservation is activated in 30~40 DEG C of water 20-40min。
Preferably, above-mentioned step（2）In, preparation steps（1）In saccharomyces cerevisiae fermentation liquid culture medium, specifically, Concentration of glucose 3-6%, yeast extract concentration 0.6-1.5% in culture medium, sterilize 25-35min at 115-126 DEG C.
Above-mentioned step（3）In, the high activity selenium-rich saccharomyces cerevisiae after activation is inoculated into zymotic fluid, inoculative proportion 1.5- 2.5%, 48h is cultivated under the conditions of 28~32 DEG C, adds 8-12g/L Asia into culture medium respectively when cultivating 12h, 24h, 36h Sodium selenate.
Above-mentioned step（5）In, blueberry pretreating process is：Blueberry is weighed, adds water, the addition of water is blueberry weight 2-5 times, 2 ~ 3h of immersion in 65-80 DEG C of water-bath is put into, is then beaten；
The pretreating process of above-mentioned other Chinese herbal medicines is：Above-mentioned other herbal components are taken, crushed 40 mesh sieves, are mixed Close, obtain Chinese herbal medicine medicinal powder, add water, the addition of water is 6-10 times of Chinese herbal medicine medicinal powder weight, and the water added is tune pH7.5 Pure water after~8.0, cellulase is then added, the addition of cellulase is the 1~2% of Chinese herbal medicine medicinal powder weight, super Extracted under sound wave subsidiary conditions.
It is furthermore preferred that above-mentioned step（5）In, blueberry pretreating process is：Blueberry is weighed, adds water, the addition of water is 3 times of blueberry weight, it is put into 75 DEG C of water-baths and soaks 2.5h, is beaten, obtains blueberry pulp；
The pretreating process of above-mentioned other Chinese herbal medicines is：Above-mentioned other herbal components are taken, crushed 40 mesh sieves, are mixed Close, obtain Chinese herbal medicine medicinal powder, add water, the addition of water is 6-10 times of Chinese herbal medicine medicinal powder weight, and water is pure after pH7.8 to adjust Water, cellulase is then added, the addition of cellulase is the 1.5% of Chinese herbal medicine medicinal powder weight, under the conditions of ultrasonic assistant Extract to obtain herbal liquid medicine；
The condition of above-mentioned ultrasonic assistant extraction is：Ultrasonication 3 times, each supersound process frequency is 20- 100Hz, each sonication treatment time 2-8min, interval time 10-40s.
Above-mentioned step（6）In, inorganic salts and its addition are specially： (NH4)2SO41.5g/L、K2HPO41.5 g/L And MgSO41 g/L, " L " of the above is every liter of mixed solution.
Above-mentioned step（8）In, fermentation step is specifically that temperature controlled fermentation, fermentation temperature control under slow stirring At 26~28 DEG C, fermentation time is 36~48h；
Using ultrasonic wave mode to yeast 20~30min of broken wall after fermentation ends, during broken wall speed of agitator 300r/min with On；
Solid insoluble is filtered, obtains wine liquid；
Ageing 3~5 days under conditions of wine liquid is placed in into 0~10 DEG C；
Wine liquid after adjustment ageing makes its alcohol by volume concentration be 10~12%, then xylitol, sorbic acid are added in wine liquid Potassium, potassium citrate, oligoisomaltose, honey；
In every liter of wine liquid each raw material is added by following weight proportion：1~1.5g xylitols, 3~5g potassium sorbates, 1~2g Potassium citrate, 5~10g oligoisomaltoses, 5~10g honey；
Membrane filtration：Micro-pore-film filtration blueberry mixed health wine；
Sterile filling：Selenium-rich blueberry mixed health wine is made in filling and sealing in sterile filling production line.
Preferably, the production technology of selenium-rich blueberry mixed health wine of the invention, including following steps：
（1）Actication of culture：The high activity selenium-rich saccharomyces cerevisiae of preservation is activated into 30min in 37 DEG C of warm water；
（2）Prepare zymotic fluid：Saccharomyces cerevisiae one-step fermentation culture medium is prepared, concentration of glucose 5%, yeast extract in culture medium Concentration 1%, 121 DEG C of sterilizing 30min, obtains zymotic fluid；
（3）Fermentation：By step（1）High activity selenium-rich saccharomyces cerevisiae is inoculated into step after middle activation（2）Zymotic fluid in, connect Kind ratio is 2%, and 48h is cultivated under the conditions of 30 DEG C, adds 10g/L Asia into culture medium respectively when cultivating 12h, 24h, 36h Sodium selenate；
（4）Filtered after fermentation ends, separate saccharomyces cerevisiae；
（5）Prepare mixed solution；
Prepare each raw material by following portions by weight：
45 parts of blueberry, 25 parts of matrimony vine, 18 parts of wilsonii, 14 parts of Ligusticum wallichii, 9 parts of barrenwort, 9 parts of the bighead atractylodes rhizome, 7 parts of the dried rhizome of rehmannia, the stem of noble dendrobium 5 Part, 4 parts of polygala, 7 parts of the fleece-flower root, 18 parts of rhodiola root, 8 parts of radix glycyrrhizae, 12 parts of STEVIA REBAUDIANA；
Blueberry pretreating process is：Blueberry is weighed, adds water, the addition of water is 3 times of blueberry weight, is put into 75 DEG C of water-baths Middle immersion 2.5h, mashing, obtains blueberry pulp；
The pretreating process of Chinese herbal medicine is：Herbal component is taken, crushed 40 mesh sieves, mixes, obtains Chinese herbal medicine medicinal powder, is added Water, the addition of water are 6-10 times of Chinese herbal medicine medicinal powder weight, and water is the pure water after tune pH7.8, then adds cellulase, The addition of cellulase is the 1.5% of Chinese herbal medicine medicinal powder weight, and herbal liquid medicine is extracted to obtain under the conditions of ultrasonic assistant；
The condition of above-mentioned ultrasonic assistant extraction is：Ultrasonication 3 times, each supersound process frequency are 60Hz, each sonication treatment time 3min, interval time 10s, extraction temperature are 65 DEG C；
（6）By step（5）In obtain blueberry pulp and mixed with Chinese herbal medicine fluid, add inorganic salts in obtained mixed solution Class, obtain Chinese herbal medicine fermentation medium；
（7）By step（4）Saccharomyces cerevisiae after middle filtering is inoculated with Chinese herbal medicine fermentation medium by 10% inoculum concentration；
（8）Fermentation, supersonic wave wall breaking, filtering, ageing, allotment, membrane filtration, sterile filling, obtain selenium-rich blueberry mixed health wine；
Comprise the following steps that：Temperature controlled fermentation under slow stirring, fermentation temperature control is at 26~28 DEG C, fermentation time For 36~48h；
Using ultrasonic wave mode to yeast 20~30min of broken wall after fermentation ends, during broken wall speed of agitator 300r/min with On；
Solid insoluble is filtered, obtains wine liquid；
Ageing 4 days under the conditions of wine liquid is placed in into 5 DEG C；
Wine liquid after adjustment ageing makes its alcohol by volume concentration 11%, then xylitol, potassium sorbate, lemon are added in wine liquid Lemon acid potassium, oligoisomaltose, honey；
In every liter of wine liquid each raw material is added in following ratio：1.2g xylitols, 4g potassium sorbates, 1.5g potassium citrates, 8g Oligoisomaltose, 8g honey；
Membrane filtration：With the micro-pore-film filtration blueberry mixed health wine that aperture is 0.15 μm；
Sterile filling：Selenium-rich blueberry mixed health wine is made in filling and sealing in sterile filling production line.
Inorganic selenium is converted into Organic Selenium by the selenium-rich blueberry mixed health wine of the present invention by Se-enriched yeast, and inorganic selenium is with oxidation state In the presence of by enteron aisle Passive intake, being reduced to selenides, be then transported to liver, synthesize the soluble shape of selenomethionine-selenium Formula, or be transported to kidney and discharged by urine.The valence state of selenium is typically with Se in Organic Selenium2-Valency exist, or with other things Matter is combined into complex state.The citation form of Organic Selenium is selenomethionine, selenium cystine etc., is existed with reduction-state, and and amino acid With reference to being absorbed with active form.Organic Selenium is significantly lower than inorganic selenium, and the natural organic selenium in meals to the toxicity of animal Reserved in animal tissue is more than inorganic selenium.People and animal test result indicates that, the biology effect of the selenium in Se-enriched yeast Power is 10~20 times of inorganic selenium, and this means that Se-enriched yeast is more suitable for improving the concentration of selenium in animal food, and this must Actively impact will be produced to the health care of the mankind.Anti-oxidant, anti-aging characteristic of the selenium-rich blueberry mixed health wine based on Organic Selenium, is equipped with Anti-oxidant, anti-aging Chinese herbal medicine, so as to develop the Organic Selenium blueberry mixed health wine for having much distinct Chinese characteristics.
For the present invention using blueberry as prescription basis, compatibility is a variety of to have anti-oxidant, senile-resistant efficacy Chinese herbal medicine, living using height Property selenium-rich fermentation by saccharomyces cerevisiae there is anti-oxidant, anti-aging, improve the Chinese herbal medicine of the various health care functions such as immunity of organisms, prepare A kind of new selenium-rich blueberry mixed health wine, the medical health care effect of the Chinese herbal medicines such as blueberry, matrimony vine was both remained, has expanded Chinese herbal medicine guarantor The application approach of strong wine, improves the organic selenium content in health liquor again, is a kind of nutritious, instant edible, unique flavor Characteristic Chinese herbal health care wine, be adapted to a variety of crowds drink, meet the needs of people are to health-care food.
The present invention, which provides a kind of selenium-rich blueberry mixed health wine and its production technology, the product of wherein selenium-rich blueberry mixed health wine, to be had Antifatigue, anti-aging, improve immunity of organisms, enhancing eyesight, anti-eye strain, skin nutrition, moistening lung, strengthening spleen and nourishing stomach, Prevent disease of capillaries, enhancing cardiac function, prevention senile dementia and other effects；The Transformation Application of the present invention can not only change Drinks consumes result, meets the needs of people is to green, safe healthcare food, is beneficial to national health, and can be high Value makes full use of Chinese herbal medicine resource, increases blueberry plant production development, increases the income of the blueberry growing area people, drives peasant to cause It is rich.
The present invention provides a kind of selenium-rich blueberry mixed health wine and its production technology, it is advantageous that：
First, inorganic selenium is converted into by security and auxotype using the two-step fermentation zymotechnique of high activity Se-enriched yeast More preferable Organic Selenium, improve the organic selenium content in health liquor, after testing in health liquor the content of Organic Selenium reach 300~ 350 μ g/L, achieve unexpected technique effect；
Second, scientific compatibility blueberry and a variety of Chinese herbal medicines, have preferable by the synergy between blueberry and Chinese herbal medicine Antifatigue, anti-aging, improve immunity of organisms, enhancing eyesight, anti-eye strain, skin nutrition, moistening lung, strengthening spleen and nourishing stomach, Prevent disease of capillaries, enhancing cardiac function, prevention senile dementia and other effects, have no side effect safely, instant.Different Chinese herbal medicine nature and flavor are different, and effect has complicated interaction relationship after being combined there is huge difference, different Chinese herbal medicines, And the proportioning between Chinese herbal medicine is closer, different ratio will also make monarch relation that substantial change occur, so that Material alterations occurs for the relation of composite Chinese herbal.
The application passes through blueberry and matrimony vine, wilsonii, Ligusticum wallichii, barrenwort, the bighead atractylodes rhizome, the dried rhizome of rehmannia, the stem of noble dendrobium, polygala, the fleece-flower root, red There is complicated interaction relationship in the Chinese herbal medicines such as red-spotted stonecrop, radix glycyrrhizae, STEVIA REBAUDIANA, rational proportion, achieve unexpected to each other Technique effect, the relation of selenium-rich blueberry mixed health wine is occurred material alterations, product TAC reaches 721.14 ± 52.17U/mL, there is preferable immunoregulatory activity；Using RNA isolation kit determination experiment mice skin tissue superoxide dismutase Enzyme（SOD）, glutathione peroxidase（GSH-Px）, MDA（MDA）And hydroxyproline（Hyp）Etc. index, the results showed that The indexs such as SOD, GSH-Px, Hyp are significantly higher than blank control group（P<0.01）, MDA is substantially less than blank control group（P<0.01）, Illustrate that product has preferable anti-aging activity；Feed change forms or proportioning can reduce the anti-oxidant of selenium-rich blueberry mixed health wine Ability and anti-aging ability（P<0.01）；
Third, by the fermentation and subsequent ultrasonic ripple broken wall synergy of high-activity yeast, soap in Chinese herbal medicine is significantly improved Glucoside, flavones, the recovery rate of alkaloid isoreactivity material, testing result show saponin, flavones, alkaloid extraction rate reached to 95% with On；
Fourth, using the production technology of micro-pore-film filtration, product clarity is high, degerming, good impurity removing effect, and sterilization effect is thorough Bottom, traditional pasteurization is instead of, nutrient component damages are small in product.
Embodiment
The present invention is further described with reference to specific embodiment, so that those skilled in the art knows more about The present invention, but and it is not so limited the present invention.
Embodiment 1
A kind of production technology of selenium-rich blueberry mixed health wine, it is characterised in that be by following method preparation：
（1）Actication of culture：The high activity selenium-rich saccharomyces cerevisiae of preservation is activated into 30min in 37 DEG C or so of warm water；
（2）Zymotic fluid is prepared：Saccharomyces cerevisiae one-step fermentation culture medium is prepared, culture medium concentration of glucose 5%, yeast extract are dense Degree 1%, 121 DEG C of sterilizing 30min, obtains zymotic fluid；
（3）Fermentation：High activity selenium-rich saccharomyces cerevisiae after activation is inoculated into step（2）Zymotic fluid in, inoculative proportion 2%, 48h is cultivated under conditions of 30 DEG C, adds 10g/L sodium selenite into culture medium respectively when cultivating 12h, 24h, 36h；
（4）Filtering：Filtered after fermentation ends, separate saccharomyces cerevisiae；
（5）Prepare raw material by following portions by weight：50 parts of blueberry, 20 parts of matrimony vine, 20 parts of wilsonii, 12 parts of Ligusticum wallichii, excessive sheep 10 parts of the leaves of pulse plants, 8 parts of the bighead atractylodes rhizome, 8 parts of the dried rhizome of rehmannia, 4 parts of the stem of noble dendrobium, 5 parts of polygala, 5 parts of the fleece-flower root, 20 parts of rhodiola root, 5 parts of radix glycyrrhizae, STEVIA REBAUDIANA 15 Part；
（6）Pretreatment of raw material：
By step（5）In parts by weight weigh each raw material；
Blueberry pretreating process is in above-mentioned raw materials：50 parts of blueberry is weighed, the water of 3 times of blueberry weight is added, is placed in 75 2.5h is soaked in DEG C water-bath, is then beaten；
The pretreating process of traditional Chinese medicinal components is in above-mentioned raw materials：Raw material mixes after crushing, and crosses 40 mesh sieves, obtains Chinese herbal medicine Medicinal powder, pure water being added, the addition of water is 8 times or so of Chinese herbal medicine medicinal powder, then using Na2CO3Regulation pure water pH be 7.8 or so, cellulase is then added, the addition weight of cellulase accounts for the 1.5% of Chinese herbal medicine weight, in ultrasonic assistant bar Extracted under part, ultrasonication 6 times, each supersound process frequency is 60Hz, each sonication treatment time 3min, during interval Between 20s；
（7）Chinese herbal medicine fermentation medium is prepared：By step（5）In obtain blueberry pulp and mixed with pretreated Chinese herbal medicine fluid Close, inorganic salts are added in obtained mixed solution, obtain Chinese herbal medicine fermentation medium, inorganic salts add in every liter of mixed solution Specific amounts be：1.5g/L (NH4)2SO4、1.5g/LK2HPO4、1g/LMgSO4；
（8）Inoculation：By step（4）Saccharomyces cerevisiae after filtering is inoculated with Chinese herbal medicine fermentation medium by 10% inoculum concentration；
Temperature controlled fermentation：Temperature controlled fermentation under slow stirring, fermentation temperature control are in 27 DEG C or so, fermentation time 40h or so；
Supersonic wave wall breaking：Using ultrasonic wave mode to yeast broken wall 25min after fermentation ends, speed of agitator during broken wall 400r/min or so；
Filtering：The solid insolubles such as Chinese herb residues and fermentation thalli are filtered to remove after fermentation ends；
Ageing：To promote the stabilization and the clarification that just carry, ageing 4 days under conditions of wine liquid is placed in into 5 DEG C or so；
Allotment：The alcohol by volume concentration 11% or so of selenium-rich blueberry mixed health wine is adjusted, adds 1.5g/L xylitols, 3g/L mountains Potassium sorbate, 2g/L potassium citrates, 5g/L oligoisomaltoses, 10g/L honey；
Membrane filtration：With the micro-pore-film filtration blueberry mixed health wine that aperture is 0.15 μm；
Sterile filling：Selenium-rich blueberry mixed health wine is made in filling and sealing in sterile filling production line.
Sensory evaluation scores：
Scored in terms of following three：Flavor 40 divides, delicate fragrance alcohol and, aroma is strong, and all tastes is coordinated, and aroma is relatively coordinated；
Mouthfeel 40 is divided, and entrance is sweet, slightly tasty and refreshing bitter taste, and aftertaste is happy smooth；
Structural state 20 divides：It is as clear as crystal, uniformity, no suspended substance and sediment.
Through 50 expert analysis modes, the health liquor average in embodiment 1 is as follows：
Flavor 39.85 divides, and 38.88 parts of mouthfeel, structural state 18.58 divides, and adds up to score：97.31 points.
The content of Organic Selenium is 330 μ g/L in above-mentioned health liquor.
Oxidation resistance is 764.14U/mL；（Using TAC detection kit detection method, i.e. (ABTS methods) The method is used in following examples, unless otherwise specified.
Select 100 women, wherein 30-40 year for 30 people, 40-50 year for 50 people, 50-60 year for 20 people, with On women have a different degrees of inferior health problem, for example sleep quality is bad causes livid ring around eye, or the colour of skin is obscure；Or Person is often easily to suffer from the diseases such as cold, tonsillitis；
100 women above take the health liquor 20mL of the present invention every nights, after one month, observe these women's The colour of skin, wherein 98 have the obvious sign for having blast, 90 sleeps take a favorable turn, and livid ring around eye mitigate；
Flu or inflammatory patients number are 0；
After two months, this 100 women are observed, wherein 99 obvious blasts of skin, and also 20 female skins become It must compact, 95 sleep states are good, and livid ring around eye mitigate；
Flu or inflammatory patients number are only 1 people.
Above-mentioned experiment shows that health liquor of the invention has good antioxidation, is highlighted for the colour of skin of women It is beneficial, and also improve immunity of organisms.
Embodiment 2
The production technology of selenium-rich blueberry mixed health wine, it is comprised the following steps that：
（1）Actication of culture：The high activity selenium-rich saccharomyces cerevisiae of preservation is activated into 30min in 37 DEG C or so warm water；
（2）Zymotic fluid is prepared：Saccharomyces cerevisiae one-step fermentation culture medium is prepared, culture medium concentration of glucose 5%, yeast extract are dense Degree 1%, 121 DEG C of sterilizing 30min, obtains zymotic fluid；
（3）One-step fermentation：High activity selenium-rich saccharomyces cerevisiae after activation is inoculated into step（2）Zymotic fluid in, be inoculated with ratio Example 2%, 48h is cultivated under the conditions of 30 DEG C, add 10g/L selenous acid into culture medium respectively when cultivating 12h, 24h, 36h Sodium；
（4）Filtering：Filtered after fermentation ends, separate saccharomyces cerevisiae；
（5）Prepare 40 parts of raw material blueberry, 30 parts of matrimony vine, 15 parts of wilsonii, 15 parts of Ligusticum wallichii, barrenwort by following portions by weight 8 parts, 10 parts of the bighead atractylodes rhizome, 6 parts of the dried rhizome of rehmannia, 6 parts of the stem of noble dendrobium, 3 parts of polygala, 10 parts of the fleece-flower root, 15 parts of rhodiola root, 10 parts of radix glycyrrhizae, STEVIA REBAUDIANA 10 Part ratio compatibility；
（6）Pretreatment of raw material：
By step（5）In parts by weight weigh each raw material：
Stating blueberry pretreating process in raw material is：50 parts of blueberry is weighed, adds 3 times of water, is placed in 75 DEG C of water-baths and soaks 2.5h, then it is beaten；
The pretreating process of medicinal herb componentses is in above-mentioned raw materials：40 mesh sieves are crushed after raw material mixing, mixes, obtains medium-height grass Medicine medicinal powder, pure water is added, the addition of water is 6 times or so of Chinese herbal medicine medicinal powder, using Na2CO3It is 7.8 to adjust pure water pH Left and right, the cellulase for accounting for that Chinese herbal medicine mass ratio is 1% is then added, 20min, extraction temperature are extracted under the conditions of ultrasonic assistant 65 DEG C or so of degree, ultrasonication 6 times, each supersound process frequency is 60Hz, each sonication treatment time 3min, interval Time 20s；
（7）Chinese herbal medicine fermentation medium is prepared：Pretreated Chinese herbal medicine is mixed, addition 1.5g/L (NH4)2SO4、 1.5g/L K2HPO4、1g/LMgSO4；
（8）Inoculation：By step（4）Saccharomyces cerevisiae after filtering is inoculated with Chinese herbal medicine fermentation medium by 10% inoculum concentration；
（9）Temperature controlled fermentation：Temperature controlled fermentation under slow stirring, fermentation temperature control is at 28 DEG C, fermentation time 36 ~48h；
（10）Supersonic wave wall breaking：Using ultrasonic wave mode to yeast broken wall 20min after fermentation ends, speed of agitator during broken wall More than 300r/min；
（11）Filtering：The solid insolubles such as Chinese herb residues and fermentation thalli are filtered to remove after fermentation ends；
（12）Ageing：To promote the stabilization and the clarification that just carry, ageing 4 days or so under conditions of wine liquid is placed in into 3 DEG C or so；
（13）Allotment：The alcohol by volume concentration 12% of selenium-rich blueberry mixed health wine is adjusted, adds 1g/L xylitols, 5g/L sorbs Sour potassium, 1g/L potassium citrates, 10g/L oligoisomaltoses, 5g/L honey；Every liter of health liquor that " L " refers to；
（14）Membrane filtration：With the micro-pore-film filtration blueberry mixed health wine that aperture is 0.15 μm；
（15）Sterile filling：Selenium-rich blueberry mixed health wine is made in filling and sealing in sterile filling production line.
Sensory evaluation scores：
Scored in terms of following three：Flavor 40 divides, delicate fragrance alcohol and, aroma is strong, and all tastes is coordinated, and aroma is relatively coordinated；
Mouthfeel 40 is divided, and entrance is sweet, slightly tasty and refreshing bitter taste, and aftertaste is happy smooth；
Structural state 20 divides：It is as clear as crystal, uniformity, no suspended substance and sediment.
Through 50 expert analysis modes, the health liquor average in embodiment 2 is as follows：
Flavor 38.85 divides, and 39.88 parts of mouthfeel, structural state 18.08 divides, and adds up to score：96.81 points.
The content of Organic Selenium is 350 μ g/L in above-mentioned health liquor.
Oxidation resistance is 780.25U/mL；
Select 100 women, wherein 30-40 year for 30 people, 40-50 year for 50 people, 50-60 year for 20 people, with On women have a different degrees of inferior health problem, for example sleep quality is bad causes livid ring around eye, or the colour of skin is obscure；Or Person is often easily to suffer from the diseases such as cold, tonsillitis；
100 women above take the health liquor 20mL of the present invention every nights, after one month, observe these women's The colour of skin, wherein 96 have the obvious sign for having blast, 93 sleeps take a favorable turn, and livid ring around eye mitigate；
Flu or inflammatory patients number are 0；
After two months, this 100 women are observed, wherein 98 obvious blasts of skin, and also 23 female skins become It must compact, 95 sleep states are good, and livid ring around eye mitigate；
Flu or inflammatory patients number are only 2 people.
Above-mentioned experiment shows that health liquor of the invention has good antioxidation, is highlighted for the colour of skin of women It is beneficial, and also improve immunity of organisms.
Embodiment 3
A kind of production technology of selenium-rich blueberry mixed health wine, it is characterised in that be by following method preparation：
（1）Actication of culture：The high activity selenium-rich saccharomyces cerevisiae of preservation is activated into 30min in 37 DEG C or so warm water；
（2）Zymotic fluid is prepared：Saccharomyces cerevisiae one-step fermentation culture medium is prepared, culture medium concentration of glucose 5%, yeast extract are dense Degree 1%, 121 DEG C of sterilizing 30min；
（3）One-step fermentation：High activity selenium-rich after activation is inoculated into zymotic fluid, inoculative proportion 2%, trained under the conditions of 30 DEG C 48h is supported, adds 10g/L sodium selenite into culture medium respectively when cultivating 12h, 24h, 36h；
（4）Filtering：Filtered after fermentation ends, separate saccharomyces cerevisiae；
（5）Prepare raw material by following portions by weight：45 parts of blueberry, 25 parts of matrimony vine, 18 parts of wilsonii, 14 parts of Ligusticum wallichii, excessive sheep 9 parts of the leaves of pulse plants, 9 parts of the bighead atractylodes rhizome, 7 parts of the dried rhizome of rehmannia, 5 parts of the stem of noble dendrobium, 4 parts of polygala, 8 parts of the fleece-flower root, 18 parts of rhodiola root, 8 parts of radix glycyrrhizae, STEVIA REBAUDIANA 12 Part；
（6）Pretreatment of raw material：
By step（5）In parts by weight weigh each raw material；
1. blueberry pretreating process is in above-mentioned raw materials：45 parts of blueberry is weighed, adds 3 times of water, is put into 75 DEG C of water-baths 2 ~ 3h is soaked, is then beaten；
2. the pretreating process of said herbal medicine raw material is：Raw material mixes after crushing, and crosses 40 mesh sieves, obtains Chinese herbal medicine Powder, pure water is added, the addition of water is 6 times or so of Chinese herbal medicine medicinal powder, using Na2CO3It is 7.5 or so to adjust pure water pH, Then the cellulase for accounting for that Chinese herbal medicine mass ratio is 1.5% is added, 25min, extraction temperature 60 are extracted under the conditions of ultrasonic assistant ~70 DEG C, obtain herbal liquid medicine；
（7）Chinese herbal medicine fermentation medium is prepared：Blueberry pulp and herbal liquid medicine mix after pre-processing, every liter of mixing is molten 1.5g/L (NH are added in liquid4)2SO4、1.5g/L K2HPO4、1g/LMgSO4；
（8）Inoculation：By step（4）Saccharomyces cerevisiae after filtering is inoculated with Chinese herbal medicine fermentation medium by 10% inoculum concentration；
（9）Temperature controlled fermentation：Temperature controlled fermentation under slow stirring, fermentation temperature control is at 26~28 DEG C, fermentation time For 36~48h；
（10）Supersonic wave wall breaking：Using ultrasonic wave mode to yeast broken wall 30min after fermentation ends, speed of agitator during broken wall More than 300r/min；
（11）Filtering：The solid insolubles such as Chinese herb residues and fermentation thalli are filtered to remove after fermentation ends；
（12）Ageing：To promote the stabilization and the clarification that just carry, ageing 3 days under conditions of wine liquid is placed in into 4 DEG C or so；
（13）Allotment：The alcohol by volume concentration 10% or so of selenium-rich blueberry mixed health wine is adjusted, adds 1.2g/L xylitols, 4g/ L potassium sorbates, 1.5g/L potassium citrates, 7.5g/L oligoisomaltoses, 7.5g/L honey；
（14）Membrane filtration：With the micro-pore-film filtration blueberry mixed health wine that aperture is 0.15 μm；
（15）Sterile filling：Selenium-rich blueberry mixed health wine is made in filling and sealing in sterile filling production line.
Sensory evaluation scores：
Scored in terms of following three：Flavor 40 divides, delicate fragrance alcohol and, aroma is strong, and all tastes is coordinated, and aroma is relatively coordinated；
Mouthfeel 40 is divided, and entrance is sweet, slightly tasty and refreshing bitter taste, and aftertaste is happy smooth；
Structural state 20 divides：It is as clear as crystal, uniformity, no suspended substance and sediment.
Through 50 expert analysis modes, the health liquor average in embodiment 1 is as follows：
Flavor 39.35 divides, and 38.86 parts of mouthfeel, structural state 18.56 divides, and adds up to score：96.77 points.
The content of Organic Selenium is 350 μ g/L in above-mentioned health liquor.
Oxidation resistance is 753.78U/mL；
Select 100 women, wherein 30-40 year for 30 people, 40-50 year for 50 people, 50-60 year for 20 people, with On women have a different degrees of inferior health problem, for example sleep quality is bad causes livid ring around eye, or the colour of skin is obscure；Or Person is often easily to suffer from the diseases such as cold, tonsillitis；
100 women above take the health liquor 20mL of the present invention every nights, after one month, observe these women's The colour of skin, wherein 97 have the obvious sign for having blast, 92 sleeps take a favorable turn, and livid ring around eye mitigate；
Flu or inflammatory patients number are 0；
After two months, this 100 women are observed, wherein 97 obvious blasts of skin, and also 22 female skins become It must compact, 96 sleep states are good, and livid ring around eye mitigate；
Flu or inflammatory patients number are only 1 people.
Above-mentioned experiment shows that health liquor of the invention has good antioxidation, is highlighted for the colour of skin of women It is beneficial, and also improve immunity of organisms.
The present invention provides a kind of selenium-rich blueberry mixed health wine and its preparation technology, and selenium-rich blueberry mixed health is carried out by animal experiment The toxicology test and safety evaluatio of wine.Chmice acute Oral toxicity result of the test shows that this selenium-rich blueberry mixed health wine is to small Mouse acute oral maximum tolerated dose is more than high dose group 75mg/kgbw；Genetic toxicity test mouse Bone marrow cells micronucleus try It is feminine gender to test with two result of the tests of mouse inbred strain, shows the selenium-rich blueberry mixed health wine without mutagenesis；By Rat 30d feeding trials understand that rat sign behavior is normal, figure is healthy, blood and blood parameters also change without exception, Therefore 75mg/kgbw dosage（150 times of human body recommended amounts）Belong to safe dose；Genetic toxicity test and rat 30d are fed Foster result of the test is feminine gender；Mouse experiment shows that this selenium-rich blueberry mixed health wine removes lipid mistake by GSH-Pxl enzymatic reactions Oxide, selenium is similar with the effect of vitamin E in growth in humans's breeding function, to maintenance organization elasticity, delays unsaturation The oxidation of aliphatic acid, prevent early stage aging from having positive role.Selenium is higher than vitamin E to the oxidation resistance of peroxidase Go out 500 times.Selenium and vitamin E constitute oxidation resistant two defense pathways of animal body, and both play a role jointly, and selenium can be with Vitamin E antioxidation is complementary to one another.Vitamin E controls unrighted acid on phosphatide not oxidized, turns into anti-oxidant The first line of defence.Selenium is catalyzed the reduction of MDA in the form of GSH-Px, in whole cell that peroxide is fast Speed resolves into alcohol and water, makes the membrane structure in cell from the infringement of peroxide, plays second defence line.It is thus determined that This selenium-rich blueberry mixed health wine belongs to nontoxic based food, and crowd can be eaten with safe and secure.
The present invention provides a kind of selenium-rich blueberry mixed health wine and its production technology, the product feature of wherein blueberry mixed health wine are The wine degree of blueberry mixed health wine be 10%~12%/(v/v), sugar content≤15g/L, the g/L of total acid (in terms of malic acid) 6~10, Volatile acid (with Acetometer)<1.2 g/L, sugar-free extract >=17 g/L, the μ g/L of organic selenium content 300~350；Blueberry mixed health wine Color and luster is in brownish red, crystal clear, no suspended substance and sediment；Entrance is soft pure and honest, and comfortable tasty and refreshing, all tastes are coordinated.
Above example is merely to illustrate technical scheme, rather than is limited；Although with reference to foregoing reality Example is applied to being described in detail by invention, but for the person of ordinary skill of the art, still can be to foregoing reality Apply the technical scheme described in example to modify, or equivalent substitution is carried out to which part technical characteristic；And to these modifications Or replace, the essence of appropriate technical solution is departed from the spirit and scope of claimed technical solution of the invention.

Claims (9)

1. a kind of production technology of selenium-rich blueberry mixed health wine, including following steps：
（1）Actication of culture：Activate selenium-rich saccharomyces cerevisiae；
（2）Zymotic fluid is prepared：Saccharomyces cerevisiae one-step fermentation culture medium is prepared, obtains zymotic fluid；
（3）Fermentation：By step（1）Selenium-rich saccharomyces cerevisiae after middle activation is inoculated into step（2）Zymotic fluid in；
（4）Filtered after fermentation ends, separate saccharomyces cerevisiae；
（5）Prepare mixed solution；
Prepare each raw material by following portions by weight：
40~50 parts of blueberry, 20~30 parts of matrimony vine, 15~20 parts of wilsonii, 12~15 parts of Ligusticum wallichii, 8~10 parts of barrenwort, the bighead atractylodes rhizome 8 ~10 parts, 6~8 parts of the dried rhizome of rehmannia, 4~6 parts of the stem of noble dendrobium, 3~5 parts of polygala, 5~10 parts of the fleece-flower root, 15~20 parts of rhodiola root, radix glycyrrhizae 5~ 10 parts, 10~15 parts of STEVIA REBAUDIANA；
Blueberry pre-processes：Blueberry is taken, adds water, is soaked, mashing, obtains blueberry pulp；
Chinese herbal medicine pre-processes：Above-mentioned other herbal components are taken, are mixed after crushing, pure water is added, then adds cellulose Enzyme, extracted under the conditions of ultrasonic assistant, obtain Chinese herbal medicine fluid；
（6）By step（5）In obtain blueberry pulp and mixed with Chinese herbal medicine fluid, add inorganic salts in obtained mixed solution, Obtain Chinese herbal medicine fermentation medium；
（7）Step will be passed through（4）Saccharomyces cerevisiae after being separated by filtration is inoculated into Chinese herbal medicine fermentation medium by 10% inoculum concentration；
（8）To step（7）In obtain culture medium fermentation, supersonic wave wall breaking, filtering, ageing, allotment, membrane filtration, sterile filling, Obtain selenium-rich blueberry mixed health wine.
A kind of 2. production technology of selenium-rich blueberry mixed health wine as claimed in claim 1, it is characterised in that described step（1） In, the high activity selenium-rich saccharomyces cerevisiae of preservation is activated into 20-40min in 30~40 DEG C of water.
A kind of 3. production technology of selenium-rich blueberry mixed health wine as claimed in claim 1, it is characterised in that described step（2） In, preparation steps（1）In saccharomyces cerevisiae one-step fermentation culture medium, actual conditions is concentration of glucose 3-6%, ferment in culture medium Female cream concentration 0.6-1.5%, sterilize 25-35min at 115-126 DEG C.
A kind of 4. production technology of selenium-rich blueberry mixed health wine as claimed in claim 1, it is characterised in that described step（3） In, the high activity selenium-rich saccharomyces cerevisiae after activation is inoculated into zymotic fluid, inoculative proportion 1.5-2.5%, under the conditions of 28~32 DEG C 48h is cultivated, adds 8-12g/L sodium selenite into culture medium respectively when cultivating 12h, 24h, 36h.
A kind of 5. production technology of selenium-rich blueberry mixed health wine as claimed in claim 1, it is characterised in that described step（5） In, blueberry pretreatment concretely comprises the following steps：Blueberry is weighed, adds water, the addition of described water is 2-5 times of blueberry weight, is put 2 ~ 3h is soaked in 65-80 DEG C of water-bath, is then beaten, obtains blueberry pulp；
The pretreating process of Chinese herbal medicine is：Remaining herbal component, 20~30 parts of matrimony vine, thorn five are taken by following parts by weight Add 15~20 parts, 12~15 parts of Ligusticum wallichii, 8~10 parts of barrenwort, 8~10 parts of the bighead atractylodes rhizome, 6~8 parts of the dried rhizome of rehmannia, 4~6 parts of the stem of noble dendrobium, polygala 3 ~5 parts, 5~10 parts of the fleece-flower root, 15~20 parts of rhodiola root, 5~10 parts of radix glycyrrhizae, 10~15 parts of STEVIA REBAUDIANA crushed 40 mesh sieves, mix Close, obtain Chinese herbal medicine medicinal powder, add water, the addition of water is 6-10 times of Chinese herbal medicine medicinal powder weight, described water for tune pH7.5~ Pure water after 8.0, cellulase then being added, the addition of described cellulase is the 1~2% of Chinese herbal medicine medicinal powder weight, Herbal liquid medicine is extracted to obtain under the conditions of ultrasonic assistant.
A kind of 6. production technology of selenium-rich blueberry mixed health wine as claimed in claim 5, it is characterised in that described step（5） In, blueberry pretreating process is：Blueberry is weighed, adds water, the addition of described water is 3 times of blueberry weight, is put into 75 DEG C of water 2.5h is soaked in bath, is beaten, obtains blueberry pulp；
The pretreating process of Chinese herbal medicine is：Herbal component is taken, crushed 40 mesh sieves, is mixed, obtains Chinese herbal medicine medicinal powder, adds water, The addition of water is 6-10 times of Chinese herbal medicine medicinal powder weight, then described water adds cellulose to adjust the pure water after pH7.8 Enzyme, the addition of described cellulase are the 1.5% of Chinese herbal medicine medicinal powder weight, and medium-height grass is extracted to obtain under the conditions of ultrasonic assistant Medicine decoction；
The condition of described ultrasonic assistant extraction is：Ultrasonication 2-8 times, each supersound process frequency is 20- 100Hz, each sonication treatment time 2-8min, interval time 10-40s.
A kind of 7. production technology of selenium-rich blueberry mixed health wine as claimed in claim 1, it is characterised in that described step（6） In, inorganic salts and its addition are specially： (NH4)2SO41.5g/L、K2HPO41.5 g/L and MgSO41 g/L, the above are inorganic The addition of salt is the weight by being added in every liter of mixed solution.
A kind of 8. production technology of selenium-rich blueberry mixed health wine as claimed in claim 1, it is characterised in that described step（8） In, fermentation step is specifically that temperature controlled fermentation, fermentation temperature control under slow stirring is in 26~28 DEG C, fermentation time 36~48h；
Using ultrasonic wave mode to yeast 20~30min of broken wall after fermentation ends, more than speed of agitator 300r/min during broken wall；
Solid insoluble is filtered, obtains wine liquid；
Ageing 3~5 days under conditions of wine liquid is placed in into 0~10 DEG C；
Adjustment ageing after wine liquid make its alcohol by volume concentration be 10~12%, then in wine liquid add xylitol, potassium sorbate, Potassium citrate, oligoisomaltose, honey；
In every liter of wine liquid each raw material is added by following weight proportion：1~1.5g xylitols, 3~5g potassium sorbates, 1~2g lemons Sour potassium, 5~10g oligoisomaltoses, 5~10g honey；
Membrane filtration：Micro-pore-film filtration blueberry mixed health wine；
Sterile filling：Selenium-rich blueberry mixed health wine is made in filling and sealing in sterile filling production line.
9. a kind of production technology of selenium-rich blueberry mixed health wine as claimed in claim 1, including following steps：
（1）Actication of culture：The high activity selenium-rich saccharomyces cerevisiae of preservation is activated into 30min in 37 DEG C of warm water；
（2）Prepare zymotic fluid：Saccharomyces cerevisiae one-step fermentation culture medium is prepared, concentration of glucose 5%, yeast extract concentration in culture medium 1%, 121 DEG C of sterilizing 30min, obtain zymotic fluid；
（3）Fermentation：By step（1）High activity selenium-rich saccharomyces cerevisiae is inoculated into step after middle activation（2）Zymotic fluid in, be inoculated with ratio Example is 2%, and 48h is cultivated under the conditions of 30 DEG C, adds 10g/L selenous acid into culture medium respectively when cultivating 12h, 24h, 36h Sodium；
（4）Filtered after fermentation ends, separate saccharomyces cerevisiae；
（5）Prepare mixed solution；
Prepare each raw material by following portions by weight：
45 parts of blueberry, 25 parts of matrimony vine, 18 parts of wilsonii, 14 parts of Ligusticum wallichii, 9 parts of barrenwort, 9 parts of the bighead atractylodes rhizome, 7 parts of the dried rhizome of rehmannia, 5 parts of the stem of noble dendrobium, 4 parts of polygala, 7 parts of the fleece-flower root, 18 parts of rhodiola root, 8 parts of radix glycyrrhizae, 12 parts of STEVIA REBAUDIANA；
Blueberry pretreating process is：Blueberry is weighed, adds water, the addition of described water is 3 times of blueberry weight, is put into 75 DEG C of water 2.5h is soaked in bath, is beaten, obtains blueberry pulp；
The pretreating process of Chinese herbal medicine is：Herbal component is taken, crushed 40 mesh sieves, is mixed, obtains Chinese herbal medicine medicinal powder, adds water, The addition of water is 6-10 times of Chinese herbal medicine medicinal powder weight, then described water adds cellulose to adjust the pure water after pH7.8 Enzyme, the addition of described cellulase are the 1.5% of Chinese herbal medicine medicinal powder weight, are extracted under the conditions of ultrasonic assistant；
The condition of described ultrasonic assistant extraction is：Ultrasonication 6 times, each supersound process frequency is 60Hz, often Secondary sonication treatment time 3min, interval time 20s；
（6）By step（5）In obtain blueberry pulp and mixed with herbal liquid medicine, add inorganic salts in obtained mixed solution Class, obtain Chinese herbal medicine fermentation medium；
（7）By step（4）Saccharomyces cerevisiae after middle filtering is inoculated with Chinese herbal medicine fermentation medium by 10% inoculum concentration；
（8）Fermentation, supersonic wave wall breaking, filtering, ageing, allotment, membrane filtration, sterile filling, obtain selenium-rich blueberry mixed health wine；
Comprise the following steps that：Temperature controlled fermentation under slow stirring, fermentation temperature control is at 26~28 DEG C, fermentation time 36 ~48h；
Using ultrasonic wave mode to yeast 20~30min of broken wall after fermentation ends, speed of agitator 300-500r/min during broken wall；
Solid insoluble is filtered, obtains wine liquid；
Ageing 4 days under the conditions of wine liquid is placed in into 5 DEG C；
Wine liquid after adjustment ageing makes its alcohol by volume concentration 11%, then xylitol, potassium sorbate, citric acid are added in wine liquid Potassium, oligoisomaltose, honey；
In every liter of wine liquid each raw material is added in following ratio：1.2g xylitols, 4g potassium sorbates, 1.5g potassium citrates, 8g are oligomeric Isomaltose, 8g honey；
Membrane filtration：With the micro-pore-film filtration blueberry mixed health wine that aperture is 0.15 μm；
Sterile filling：Selenium-rich blueberry mixed health wine is made in filling and sealing in sterile filling production line.
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CN105820912A (en) * 2016-05-05 2016-08-03 安徽味多多餐饮管理有限公司 Selenium-rich blueberry healthcare wine and preparing method thereof
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