CN105475127A - Plant mutagenic agent and preparation and use methods thereof - Google Patents
Plant mutagenic agent and preparation and use methods thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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Abstract
The invention discloses a plant mutagenic agent used for polyploid breeding and culturing of plants. The mutagenic agent is prepared from heavy water, nuclear reactor wastewater, mining wastewater, zirconium-indium sand, ethylmethane sulfonate, colchicine, acenaphthene, fumiron, heteroauxin, naphtho-ethane, ammonium dihydrogen phosphate, diammonium phosphate, monopotassium phosphate, potassium nitrate, ammonium nitrate, ethylenediaminetetraacetic acid, oxalic acid penteticacid, 1,2-cyclohexanediamine tetraacetic acid, 2-hydroxyphenylacetic acid, hydroxyethyl ethylenediamine triacetic acid and tap water. The plant mutagenic agent comprises a solid part and a liquid part; the solid part is used for embedding seeds, and the zirconium-indium sand serves as the solid part; and the liquid part is used for watering seedlings. The solid and liquid cooperating mutagenesis mode is adopted, so that the mutagenic effect is better; the zirconium-indium sand containing rich radioactive substances is adopted as a matrix for culturing seeds, so that the mutagenic efficiency is easily improved.
Description
Technical field
The invention belongs to field of plant breeding, particularly relate to a kind of plant mutagen and make using method.
Background technology
Utilizing mutating technology rice to create, useful variation has been proved is the very potential breeding method of one.So far the whole world has been selected 2000 excellent mutating varieties and has been applied aborning in 150 various plants.And in cultured in vitro, doubled haploid technique is then the more effective method promoting mutating technology application.Along with going deep into of research, the abductive approach of existing polyploid can constantly improve, maximum chemical induction method of especially producing.And the large multioperation of present mutagen uses complicated, be difficult to as fertilizer irrigation easy to use.Therefore, be necessary to develop a kind of mutagen that can be easy to use, to meet the demand of market for plant mutagen.
Chinese patent CN201510099586.6 discloses the tetraploid method of a kind of live body southern high clump cowberry of induction, take root seedling for material with plantlet in vitro, adopt live body revulsion to carry out tetraploid induction to south high clump blueberry tissue culture seedling, obtain strong stress resistance, high-quality, high yield, south height clump cowberry plant that single fruit is large.But the method complicated operation, needs breeder to take much time and energy carries out breeding work, and breeding efficiency is low.
Chinese patent CN201410766003.6 discloses a kind of tomato juice root system mutagen containing salicylic acid biology enzyme, is made up: willow branch 40%, rape flower honey 10% part, rice bran vinegar liquid 40%, spirulina extract 10%, complexing formula nanometer ammonia state copper microballoon 1%, fermentative microflora 0.1-0.2 form of the raw material of following percentage by weight.But the method can not realize the polyploid breeding of plant, restricted application.
Therefore be badly in need of a kind ofly can realizing polyploid breeding, breeding efficiency plant mutagen high and easy to use, to adapt to plant demand widely.
Summary of the invention
The present invention is directed to the problems referred to above, a kind of plant mutagen are provided, cultivate for plant polyploid breeding, mutagen comprise heavy water, nuclear reactor waste water, mining wastewater, zirconium indium sand, ethylmethane sulfonate, colchicine, acenaphthene, Fu Mingnong, different growth hormone, naphthalene a pair of horses going side by side ethane, ammonium dihydrogen phosphate (ADP), diammonium phosphate, potassium dihydrogen phosphate, potassium nitrate, ammonium nitrate, ethylenediamine tetra-acetic acid, oxalic acid pentaacetic acid, 1, 2-1,2-diaminocyclohexane tetraacetic acid, ortho-hydroxyphenyl acetic acid, hydroxyethylethylene diamine tri-acetic acid, running water, plant mutagen are divided into solid portion and liquid part, solid portion is used for the embedding of seed, solid portion is zirconium indium sand, liquid part is used for seedling pouring.Adopt solid and liquid to coordinate the mode of mutagenesis, make Mutagenic Effect better, adopt the zirconium indium sand being rich in radiogen as the base-material cultivating seed, be conducive to improving efficiency of inducing mutation.
Further, the weight portion of mutagen consists of: 20 parts ~ 30 parts, heavy water, nuclear reactor waste water 20 parts ~ 30 parts, mining wastewater 20 parts ~ 30 parts, 200 parts ~ 300 parts, zirconium indium sand, ethylmethane sulfonate 0.2 part ~ 0.3 part, colchicine 0.2 part ~ 0.4 part, acenaphthene 0.1 part ~ 0.3 part, Fu Mingnong 0.1 part ~ 0.3 part, different growth hormone 0.2 part ~ 0.3 part, naphthalene a pair of horses going side by side 0.1 part ~ 0.4 part, ethane, ammonium dihydrogen phosphate (ADP) 2 parts ~ 3 parts, diammonium phosphate 1 part ~ 3 parts, potassium dihydrogen phosphate 1 part ~ 3 parts, 2 parts ~ 4 parts, potassium nitrate, 1 part ~ 3 parts, ammonium nitrate, ethylenediamine tetra-acetic acid 1 part ~ 5 parts, oxalic acid pentaacetic acid 1 part ~ 3 parts, 1, 2-1,2-diaminocyclohexane tetraacetic acid 2 parts ~ 5 parts, ortho-hydroxyphenyl acetic acid 1 part ~ 4 parts, hydroxyethylethylene diamine tri-acetic acid 1 part ~ 3 parts, 2000 parts ~ 3000 parts, running water.
Further, heavy water to be mass fraction be 60% ~ 70% heavy water.The growth of heavy water to seed has a great impact, too high heavy water content can produce inhibitory action to the growth of seed, the heavy water of 60% ~ 70% reduces according to concentration after the weight ratio of 20 parts ~ 30 parts and other components matching, can not have a negative impact to the growth of seed, with the uncertain impact that other compositions of limit produce plant seed mutagenesis, mutagenesis is evenly carried out, and plant evenly grows.
Further, liquid part is divided into nutrition type to water agent and mutagenesis type pouring agent, nutrition type pouring agent is mining wastewater, ammonium dihydrogen phosphate (ADP), diammonium phosphate, potassium dihydrogen phosphate, potassium nitrate, ammonium nitrate, ethylenediamine tetra-acetic acid, oxalic acid pentaacetic acid, 1, the mixture of 2-1,2-diaminocyclohexane tetraacetic acid, ortho-hydroxyphenyl acetic acid, hydroxyethylethylene diamine tri-acetic acid, running water, mutagenesis type pouring agent is the mixture of ethylmethane sulfonate, colchicine, acenaphthene, Fu Mingnong, different growth hormone, naphthalene a pair of horses going side by side ethane, heavy water, nuclear reactor waste water, running water.
Further, the compound method of nutrition type pouring agent is: by ammonium dihydrogen phosphate (ADP) 2 parts ~ 3 parts, diammonium phosphate 1 part ~ 3 parts, potassium dihydrogen phosphate 1 part ~ 3 parts, 2 parts ~ 4 parts, potassium nitrate, 1 part ~ 3 parts, ammonium nitrate, add in 1000 parts ~ 1500 parts, running water successively, obtain a homogeneous mixture, then mining wastewater 20 parts ~ 30 parts joins in mixture after mixing with ethylenediamine tetra-acetic acid 1 part ~ 5 parts, oxalic acid pentaacetic acid 1 part ~ 3 parts, CDTA 2 parts ~ 5 parts, ortho-hydroxyphenyl acetic acid 1 part ~ 4 parts, hydroxyethylethylene diamine tri-acetic acid 1 part ~ 3 parts.The growth that nutrition type pouring agent is seed and the growth of seedling provide necessary nutriment, ethylenediamine tetra-acetic acid, oxalic acid pentaacetic acid, 1 in nutrition type pouring agent, 2-1,2-diaminocyclohexane tetraacetic acid, ortho-hydroxyphenyl acetic acid, hydroxyethylethylene diamine tri-acetic acid are complex compound, the metal ion in absorption pouring agent can be crossed, promote growing of plant.
Further, the compound method of mutagenesis type pouring agent is: after ethylmethane sulfonate 0.2 part ~ 0.3 part, colchicine 0.2 part ~ 0.4 part, acenaphthene 0.1 part ~ 0.3 part, rich bright agriculture 0.1 part ~ 0.3 part, different growth hormone 0.2 part ~ 0.3 part, naphthalene a pair of horses going side by side 0.1 part ~ 0.4 part, ethane being mixed with nuclear reactor waste water 20 parts ~ 30 parts, then 20 parts ~ 30 parts, heavy water is added while stirring, finally add 1000 parts ~ 1500 parts, running water, mix.Mutagenesis type pouring agent chemical mutagen and nuclear industry waste water combine preparation, namely improve the efficiency of inducing mutation of plant seed, also alleviate the environmental pollution near nuclear power plant to a certain extent.
According to another object of the present invention, be the preparation method providing a kind of plant mutagen, preparation method is:
The first step, mutagenesis type pouring agent preparation: after ethylmethane sulfonate 0.2 part ~ 0.3 part, colchicine 0.2 part ~ 0.4 part, acenaphthene 0.1 part ~ 0.3 part, rich bright agriculture 0.1 part ~ 0.3 part, different growth hormone 0.2 part ~ 0.3 part, naphthalene a pair of horses going side by side 0.1 part ~ 0.4 part, ethane are mixed with nuclear reactor waste water 20 parts ~ 30 parts, then 20 parts ~ 30 parts, heavy water is added while stirring, finally add 1000 parts ~ 1500 parts, running water, mix;
The compound method of second step, nutrition type pouring agent is: by ammonium dihydrogen phosphate (ADP) 2 parts ~ 3 parts, diammonium phosphate 1 part ~ 3 parts, potassium dihydrogen phosphate 1 part ~ 3 parts, 2 parts ~ 4 parts, potassium nitrate, 1 part ~ 3 parts, ammonium nitrate, add in 1000 parts ~ 1500 parts, running water successively, obtain a homogeneous mixture, then mining wastewater 20 parts ~ 30 parts joins in mixture with ethylenediamine tetra-acetic acid 1 part ~ 5 parts, oxalic acid pentaacetic acid 1 part ~ 3 parts, CDTA 2 parts ~ 5 parts, ortho-hydroxyphenyl acetic acid 1 part ~ 4 parts, hydroxyethylethylene diamine tri-acetic acid 1 part ~ 3 parts after mixing;
3rd step, finished product packing: zirconium indium sand is dipped in nutrition type pouring agent, then plastic sack vacuum sealed package is used, obtain sub-material packaging one, again nutrition type is watered agent vacuum sealed package, obtain sub-material packaging two, finally 1 bag of sub-material packaging one and 1 bag of sub-material packaging two are put together, with plastic bag packaging sealing, obtain mutagen finished product.
Further, under plant mutagen are kept at 0 DEG C ~ 4 DEG C conditions.Zirconium indium sand is dipped into nutrition type pouring agent and makes that nutriment is easier to be absorbed along with the sprouting of seed; Mutagen separate independent packaging and under being kept at 0 DEG C ~ 4 DEG C conditions, are conducive to the stability of plant mutagen.
Of the present invention also have an object, be to provide a kind of using method of plant mutagen be: to be taken out from nutrition type pouring agent by zirconium indium sand, seed is embedded in zirconium indium sand, water mutagenesis type pouring agent every day, keep zirconium indium sand moistening but not ponding, after seed germination, the alternately pouring agent of pouring nutrition type and mutagenesis type pouring agent, use to mutagen, then seedling is transplanted out from zirconium indium sand.Using method is simple and easy to do, the mutagenesis of plant seed operation is safeguarded combining with daily irrigation, decreases hand labor degree, improve mutagenesis operating efficiency.
Further, zirconium indium sand can reclaim and reuse, and economizes on resources.
Advantage of the present invention is:
1. contain heavy water in mutagen of the present invention, but after heavy water and other components matching, concentration reduces, and can not have a negative impact to the growth of seed, the uncertain impact produced plant seed mutagenesis with other compositions of limit, mutagenesis is evenly carried out, and plant evenly grows;
2. in mutagen of the present invention, mutagenesis type pouring agent chemical mutagen and nuclear industry waste water combine preparation, namely improve the efficiency of inducing mutation of plant seed, also alleviate the environmental pollution near nuclear power plant to a certain extent;
3. the present invention prepare, pack, preserve scientific and reasonable, the good stability of the plant mutagen of preparation;
4. using method of the present invention is simple and easy to do, the mutagenesis of plant seed operation is safeguarded combining with daily irrigation, decreases hand labor degree, improve mutagenesis operating efficiency.
Except object described above, feature and advantage, the present invention also has other object, feature and advantage.Below with reference to embodiment, the present invention is further detailed explanation.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail, but the multitude of different ways that the present invention can be defined by the claims and cover is implemented.
Embodiment 1
A kind of plant mutagen
Cultivate for plant polyploid breeding, mutagen are divided into solid portion and liquid part, and solid portion is used for the embedding of seed, and solid portion is zirconium indium sand; Liquid part is used for seedling pouring.The weight portion of mutagen consists of: mass fraction is the heavy water 25kg of 65%, nuclear reactor waste water 25kg, mining wastewater 25kg, zirconium indium sand 250kg, ethylmethane sulfonate 0.25kg, colchicine 0.3kg, acenaphthene 0.2kg, Fu Mingnong 0.2kg, different growth hormone 0.25kg, naphthalene a pair of horses going side by side ethane 0.25kg, ammonium dihydrogen phosphate (ADP) 2.5kg, diammonium phosphate 2kg, potassium dihydrogen phosphate 2kg, potassium nitrate 3kg, ammonium nitrate 2kg, ethylenediamine tetra-acetic acid 3kg, oxalic acid pentaacetic acid 2kg, 1, 2-1,2-diaminocyclohexane tetraacetic acid 3.5kg, ortho-hydroxyphenyl acetic acid 2.5kg, hydroxyethylethylene diamine tri-acetic acid 2kg, running water 2500kg.
Embodiment 2
A kind of plant mutagen
Cultivate for plant polyploid breeding, mutagen are divided into solid portion and liquid part, and solid portion is used for the embedding of seed, and solid portion is zirconium indium sand; Liquid part is used for seedling pouring.The weight portion of mutagen consists of: mass fraction is the heavy water 20kg of 60%, nuclear reactor waste water 20kg, mining wastewater 20kg, zirconium indium sand 200kg, ethylmethane sulfonate 0.2kg, colchicine 0.2kg, acenaphthene 0.1kg, Fu Mingnong 0.1kg, different growth hormone 0.2kg, naphthalene a pair of horses going side by side ethane 0.1kg, ammonium dihydrogen phosphate (ADP) 2kg, diammonium phosphate 1kg, potassium dihydrogen phosphate 1kg, potassium nitrate 2kg, ammonium nitrate 1kg, ethylenediamine tetra-acetic acid 1kg, oxalic acid pentaacetic acid 1kg, 1, 2-1,2-diaminocyclohexane tetraacetic acid 2kg, ortho-hydroxyphenyl acetic acid 1kg, hydroxyethylethylene diamine tri-acetic acid 1kg, running water 2000kg.
Embodiment 3
A kind of plant mutagen
Cultivate for plant polyploid breeding, mutagen are divided into solid portion and liquid part, and solid portion is used for the embedding of seed, and solid portion is zirconium indium sand; Liquid part is used for seedling pouring.The weight portion of mutagen consists of: mass fraction is the heavy water 30kg of 70%, nuclear reactor waste water 30kg, mining wastewater 30kg, zirconium indium sand 300kg, ethylmethane sulfonate 0.3kg, colchicine 0.4kg, acenaphthene 0.3kg, Fu Mingnong 0.3kg, different growth hormone 0.3kg, naphthalene a pair of horses going side by side ethane 0.4kg, ammonium dihydrogen phosphate (ADP) 3kg, diammonium phosphate 3kg, potassium dihydrogen phosphate 3kg, potassium nitrate 4kg, ammonium nitrate 3kg, ethylenediamine tetra-acetic acid 5kg, oxalic acid pentaacetic acid 3kg, 1, 2-1,2-diaminocyclohexane tetraacetic acid 5kg, ortho-hydroxyphenyl acetic acid 4kg, hydroxyethylethylene diamine tri-acetic acid 3kg, running water 3000kg.
Embodiment 4
The preparation method of plant mutagen
Preparation method is:
The first step, mutagenesis type pouring agent preparation: after ethylmethane sulfonate 0.2kg, colchicine 0.2kg, acenaphthene 0.1kg, rich bright agriculture 0.1kg, different growth hormone 0.2kg, naphthalene a pair of horses going side by side ethane 0.1kg are mixed with nuclear reactor waste water 20kg, then heavy water 20kg is added while stirring, finally add running water 1000kg, mix.
The compound method of second step, nutrition type pouring agent is: by ammonium dihydrogen phosphate (ADP) 2 parts ~ 3 parts, diammonium phosphate 1 part ~ 3 parts, potassium dihydrogen phosphate 1 part ~ 3 parts, 2 parts ~ 4 parts, potassium nitrate, 1 part ~ 3 parts, ammonium nitrate, add in 100 parts ~ 150 parts, running water successively, obtain a homogeneous mixture, then mining wastewater 20 parts ~ 30 parts joins in mixture after mixing with ethylenediamine tetra-acetic acid 1 part ~ 5 parts, oxalic acid pentaacetic acid 1 part ~ 3 parts, CDTA 2 parts ~ 5 parts, ortho-hydroxyphenyl acetic acid 1 part ~ 4 parts, hydroxyethylethylene diamine tri-acetic acid 1 part ~ 3 parts.
3rd step, finished product packing: zirconium indium sand is dipped in nutrition type pouring agent, then plastic sack vacuum sealed package is used, obtain sub-material packaging one, again nutrition type is watered agent vacuum sealed package, obtain sub-material packaging two, finally 1 bag of sub-material packaging one and 1 bag of sub-material packaging two are put together, seal with plastic bag packaging, obtain mutagen finished product, preserve under 0 DEG C ~ 4 DEG C conditions.
Embodiment 5
A kind of using method of plant mutagen
Using method is: taken out from nutrition type pouring agent by zirconium indium sand, seed is embedded in zirconium indium sand, water mutagenesis type pouring agent every day, keep zirconium indium sand moistening but not ponding, after seed germination, alternately the pouring agent of pouring nutrition type and mutagenesis type pouring agent, use to mutagen, then transplant out from zirconium indium sand by seedling, wherein zirconium indium sand can reclaim and reuse.
Experimental example
In this experimental example, test the Mutagenic Effect of the present invention 1 ~ 3 mutagen by experiment.
1. test material
Same tomato seeds 4kg, 3kg are as experimental group, and as a control group, experiment component is three groups to 1kg, often organizes 1kg.
Experimental group: according to growing state and the output of seedling after the embodiment of the present invention 1 ~ 3 mutagen;
Control group: the Growth of Tomato Seedling situation obtained with the process of common colchicine method of mutagenesis and output.
2. test method
Test the situation statistics of Growth of Tomato Seedling situation that experimental example of the present invention and reference examples obtain and output.
3. result of the test
Analyze after the situation statistics of the Growth of Tomato Seedling situation after the embodiment of the present invention 1 ~ 3 mutagen, the Growth of Tomato Seedling situation obtained with common colchicine process and output, experimental result is in table 1.
Table 1. test result
| Material | Minimum tolerable temperature (DEG C) | Result situation | Growing state |
| Tomato seedling after embodiment 1 mutagen | -7 | Individuality is large, quantity is many, fruit difference is little | Growing way is fast, grow uniform and stable, robust plant |
| Tomato seedling after embodiment 2 mutagen | -8 | Individuality is large, quantity is many, fruit difference is little | Growing way is fast, grow uniform and stable, robust plant 4--> |
| Tomato seedling after embodiment 3 mutagen | -6 | Individuality is large, quantity is many, fruit difference is little | Growing way is fast, grow uniform and stable, robust plant |
| Tomato seedling after common colchicine mutagenesis | -4 | Individual large, quantity is not etc., fruit difference is large | Growing way is fast, it is uneven to grow, plant otherness is large |
Experimental result shows, compared with the tomato seedling of cultivating with common colchicine, the result situation of the tomato seedling that the minimum tolerable temperature of the tomato seedling that the minimum tolerable temperature of tomato seedling is cultivated than common colchicine under square one of the tomato seedling after the embodiment of the present invention 1 ~ 3 mutagen is low, result situation is cultivated than common colchicine is good, and the tomato seedling that growing state is cultivated than common colchicine is more stable, neat, otherness is less.
These are only the preferred embodiments of the present invention and experimental example, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., within the protection domain that all should be included in invention.
Claims (10)
1. plant mutagen, cultivate for plant polyploid breeding, it is characterized in that, described mutagen comprise heavy water, nuclear reactor waste water, mining wastewater, zirconium indium sand, ethylmethane sulfonate, colchicine, acenaphthene, Fu Mingnong, different growth hormone, naphthalene a pair of horses going side by side ethane, ammonium dihydrogen phosphate (ADP), diammonium phosphate, potassium dihydrogen phosphate, potassium nitrate, ammonium nitrate, ethylenediamine tetra-acetic acid, oxalic acid pentaacetic acid, 1, 2-1,2-diaminocyclohexane tetraacetic acid, ortho-hydroxyphenyl acetic acid, hydroxyethylethylene diamine tri-acetic acid, running water, described plant mutagen are divided into solid portion and liquid part, described solid portion is used for the embedding of seed, described solid portion is zirconium indium sand, described liquid part is used for seedling pouring.
2. plant mutagen according to claim 1, it is characterized in that, the weight portion of described mutagen consists of: 20 parts ~ 30 parts, heavy water, nuclear reactor waste water 20 parts ~ 30 parts, mining wastewater 20 parts ~ 30 parts, 200 parts ~ 300 parts, zirconium indium sand, ethylmethane sulfonate 0.2 part ~ 0.3 part, colchicine 0.2 part ~ 0.4 part, acenaphthene 0.1 part ~ 0.3 part, Fu Mingnong 0.1 part ~ 0.3 part, different growth hormone 0.2 part ~ 0.3 part, naphthalene a pair of horses going side by side 0.1 part ~ 0.4 part, ethane, ammonium dihydrogen phosphate (ADP) 2 parts ~ 3 parts, diammonium phosphate 1 part ~ 3 parts, potassium dihydrogen phosphate 1 part ~ 3 parts, 2 parts ~ 4 parts, potassium nitrate, 1 part ~ 3 parts, ammonium nitrate, ethylenediamine tetra-acetic acid 1 part ~ 5 parts, oxalic acid pentaacetic acid 1 part ~ 3 parts, 1, 2-1,2-diaminocyclohexane tetraacetic acid 2 parts ~ 5 parts, ortho-hydroxyphenyl acetic acid 1 part ~ 4 parts, hydroxyethylethylene diamine tri-acetic acid 1 part ~ 3 parts, 2000 parts ~ 3000 parts, running water.
3. plant mutagen according to claim 1, is characterized in that, described heavy water to be mass fraction be 60% ~ 70% heavy water.
4. plant mutagen according to claim 1, it is characterized in that, described liquid part is divided into nutrition type to water agent and mutagenesis type pouring agent, described nutrition type pouring agent is mining wastewater, ammonium dihydrogen phosphate (ADP), diammonium phosphate, potassium dihydrogen phosphate, potassium nitrate, ammonium nitrate, ethylenediamine tetra-acetic acid, oxalic acid pentaacetic acid, 1, 2-1,2-diaminocyclohexane tetraacetic acid, ortho-hydroxyphenyl acetic acid, hydroxyethylethylene diamine tri-acetic acid, the mixture of running water, described mutagenesis type pouring agent is ethylmethane sulfonate, colchicine, acenaphthene, Fu Mingnong, different growth hormone, naphthalene a pair of horses going side by side ethane, heavy water, nuclear reactor waste water, the mixture of running water.
5. plant mutagen according to claim 4, it is characterized in that, the compound method of described nutrition type pouring agent is: by ammonium dihydrogen phosphate (ADP) 2 parts ~ 3 parts, diammonium phosphate 1 part ~ 3 parts, potassium dihydrogen phosphate 1 part ~ 3 parts, 2 parts ~ 4 parts, potassium nitrate, 1 part ~ 3 parts, ammonium nitrate, add in 1000 parts ~ 1500 parts, running water successively, obtain a homogeneous mixture, then mining wastewater 20 parts ~ 30 parts and ethylenediamine tetra-acetic acid 1 part ~ 5 parts, oxalic acid pentaacetic acid 1 part ~ 3 parts, 1, 2-1,2-diaminocyclohexane tetraacetic acid 2 parts ~ 5 parts, ortho-hydroxyphenyl acetic acid 1 part ~ 4 parts, join in mixture after hydroxyethylethylene diamine tri-acetic acid 1 part ~ 3 parts mixes.
6. plant mutagen according to claim 4, it is characterized in that, the compound method of described mutagenesis type pouring agent is: after ethylmethane sulfonate 0.2 part ~ 0.3 part, colchicine 0.2 part ~ 0.4 part, acenaphthene 0.1 part ~ 0.3 part, rich bright agriculture 0.1 part ~ 0.3 part, different growth hormone 0.2 part ~ 0.3 part, naphthalene a pair of horses going side by side 0.1 part ~ 0.4 part, ethane being mixed with nuclear reactor waste water 20 parts ~ 30 parts, then 20 parts ~ 30 parts, heavy water is added while stirring, finally add 1000 parts ~ 1500 parts, running water, mix.
7. a preparation method for the plant mutagen as described in any one of claim 1 ~ 6, is characterized in that, described preparation method is:
The first step, mutagenesis type pouring agent preparation: after ethylmethane sulfonate 0.2 part ~ 0.3 part, colchicine 0.2 part ~ 0.4 part, acenaphthene 0.1 part ~ 0.3 part, rich bright agriculture 0.1 part ~ 0.3 part, different growth hormone 0.2 part ~ 0.3 part, naphthalene a pair of horses going side by side 0.1 part ~ 0.4 part, ethane are mixed with nuclear reactor waste water 20 parts ~ 30 parts, then 20 parts ~ 30 parts, heavy water is added while stirring, finally add 1000 parts ~ 1500 parts, running water, mix;
The compound method of second step, nutrition type pouring agent is: by ammonium dihydrogen phosphate (ADP) 2 parts ~ 3 parts, diammonium phosphate 1 part ~ 3 parts, potassium dihydrogen phosphate 1 part ~ 3 parts, 2 parts ~ 4 parts, potassium nitrate, 1 part ~ 3 parts, ammonium nitrate, add in 1000 parts ~ 1500 parts, running water successively, obtain a homogeneous mixture, then mining wastewater 20 parts ~ 30 parts joins in mixture with ethylenediamine tetra-acetic acid 1 part ~ 5 parts, oxalic acid pentaacetic acid 1 part ~ 3 parts, CDTA 2 parts ~ 5 parts, ortho-hydroxyphenyl acetic acid 1 part ~ 4 parts, hydroxyethylethylene diamine tri-acetic acid 1 part ~ 3 parts after mixing;
3rd step, finished product packing: zirconium indium sand is dipped in nutrition type pouring agent, then plastic sack vacuum sealed package is used, obtain sub-material packaging one, again nutrition type is watered agent vacuum sealed package, obtain sub-material packaging two, finally 1 bag of sub-material packaging one and 1 bag of sub-material packaging two are put together, with plastic bag packaging sealing, obtain mutagen finished product.
8. plant mutagen according to claim 1, is characterized in that, under described plant mutagen are kept at 0 DEG C ~ 4 DEG C conditions.
9. the using method of plant mutagen, it is characterized in that, described using method is: taken out from nutrition type pouring agent by zirconium indium sand, seed is embedded in zirconium indium sand, waters mutagenesis type pouring agent every day, keep zirconium indium sand moistening but not ponding, after seed germination, alternately the pouring agent of pouring nutrition type and mutagenesis type pouring agent, use to mutagen, then transplant out by seedling from zirconium indium sand.
10. the plant mutagen according to any one of claim 1 ~ 8, is characterized in that, described zirconium indium sand can reclaim and reuse.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107173221A (en) * | 2017-07-11 | 2017-09-19 | 中国农业大学 | Seed automatic soaking cleaning device |
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