CN105473610A - 用于pth的纯化方法 - Google Patents

用于pth的纯化方法 Download PDF

Info

Publication number
CN105473610A
CN105473610A CN201480046603.3A CN201480046603A CN105473610A CN 105473610 A CN105473610 A CN 105473610A CN 201480046603 A CN201480046603 A CN 201480046603A CN 105473610 A CN105473610 A CN 105473610A
Authority
CN
China
Prior art keywords
exchange chromatography
anion exchange
rhpth
column
pth
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201480046603.3A
Other languages
English (en)
Chinese (zh)
Inventor
桑吉乌·库马尔·门迪拉塔
桑贾伊·班迪奥帕迪亚伊
阿万尼什·库马尔·辛格
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zydus Lifesciences Ltd
Original Assignee
Cadila Healthcare Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cadila Healthcare Ltd filed Critical Cadila Healthcare Ltd
Publication of CN105473610A publication Critical patent/CN105473610A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/635—Parathyroid hormone, i.e. parathormone; Parathyroid hormone-related peptides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Endocrinology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
CN201480046603.3A 2013-08-21 2014-08-21 用于pth的纯化方法 Pending CN105473610A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IN2726/MUM/2013 2013-08-21
IN2726MU2013 IN2013MU02726A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 2013-08-21 2014-08-21
PCT/IN2014/000539 WO2015025335A1 (en) 2013-08-21 2014-08-21 Purification process for pth

Publications (1)

Publication Number Publication Date
CN105473610A true CN105473610A (zh) 2016-04-06

Family

ID=51862491

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480046603.3A Pending CN105473610A (zh) 2013-08-21 2014-08-21 用于pth的纯化方法

Country Status (16)

Country Link
US (1) US20160200792A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EP (1) EP3036252A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (1) JP6046862B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
KR (1) KR101687686B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CN (1) CN105473610A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
AU (1) AU2014310255B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CA (1) CA2919604A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EA (1) EA201690264A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
HK (1) HK1223378A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IL (1) IL243642A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IN (1) IN2013MU02726A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
MX (1) MX2016002274A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
NZ (1) NZ716205A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
SG (1) SG11201600511QA (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
WO (1) WO2015025335A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
ZA (1) ZA201600424B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107941983A (zh) * 2018-01-05 2018-04-20 北京博康健基因科技有限公司 一种rhPTH蛋白或rhPTH蛋白制剂的检测方法和纯度的分析方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3012952B1 (fr) * 2013-11-08 2015-11-06 Essilor Int Methode de determination d'au moins un parametre de conception optique d'une lentille ophtalmique progressive
WO2019077432A1 (en) * 2017-10-16 2019-04-25 Intas Pharmaceuticals Ltd. IMPROVED PURIFICATION METHOD OF RECOMBINANT PTH (1-34)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1706947A (zh) * 2004-06-04 2005-12-14 南京大学生物制药工程研究中心 重组人甲状旁腺激素在大肠杆菌中的构建、表达与纯化方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988000596A1 (en) * 1986-07-18 1988-01-28 The University Of Melbourne A PROTEIN ACTIVE IN HUMORAL HYPERCALCEMIA OF MALIGNANCY-PTHrP
US5171670A (en) * 1989-05-12 1992-12-15 The General Hospital Corporation Recombinant dna method for production of parathyroid hormone
DE3935738A1 (de) * 1989-10-27 1991-05-08 Forssmann Wolf Georg Arzneimittel, enthaltend das humane parathormon-fragment (1-37) als aktiven wirkstoff
EP0812856B1 (en) * 1996-06-14 1999-09-29 Takeda Chemical Industries, Ltd. Method for removing N-terminal methionine
AU7701398A (en) * 1997-05-30 1998-12-30 Bion, Inc. Large-scale isolation and purification of hif
WO2003102132A2 (en) 2002-04-26 2003-12-11 Genetech, Inc. Non-affinity purification of proteins
EP1598075A1 (en) * 2004-05-21 2005-11-23 LEK Pharmaceuticals d.d. Process for the isolation and / or purification of proteins
DE602006014126D1 (de) * 2005-04-20 2010-06-17 Viromed Co Ltd Zusammensetzungen und verfahren zur trennung von fusionsproteinen
CN1861790A (zh) * 2006-04-25 2006-11-15 华东理工大学 重组人甲状旁腺激素1－84的制备方法
CA2694562A1 (en) * 2007-08-09 2009-02-12 Usv Limited Novel orthogonal process for purification of recombinant human parathyroid hormone (rhpth) (1-34)
WO2011151714A1 (en) * 2010-06-04 2011-12-08 Lupin Limited Modified sak gene for the production of recombinant proteins
SG10201508401TA (en) * 2010-10-11 2015-11-27 Abbvie Bahamas Ltd Processes for purification of proteins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1706947A (zh) * 2004-06-04 2005-12-14 南京大学生物制药工程研究中心 重组人甲状旁腺激素在大肠杆菌中的构建、表达与纯化方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107941983A (zh) * 2018-01-05 2018-04-20 北京博康健基因科技有限公司 一种rhPTH蛋白或rhPTH蛋白制剂的检测方法和纯度的分析方法

Also Published As

Publication number Publication date
CA2919604A1 (en) 2015-02-26
AU2014310255A1 (en) 2016-02-25
IN2013MU02726A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 2015-06-26
WO2015025335A8 (en) 2016-02-04
JP2016528173A (ja) 2016-09-15
KR20160027204A (ko) 2016-03-09
IL243642A0 (en) 2016-03-31
HK1223378A1 (zh) 2017-07-28
EP3036252A1 (en) 2016-06-29
AU2014310255B2 (en) 2017-02-16
MX2016002274A (es) 2016-05-31
WO2015025335A1 (en) 2015-02-26
EA201690264A1 (ru) 2016-06-30
ZA201600424B (en) 2017-03-29
US20160200792A1 (en) 2016-07-14
IL243642A (en) 2017-05-29
KR101687686B1 (ko) 2016-12-19
SG11201600511QA (en) 2016-02-26
NZ716205A (en) 2017-05-26
JP6046862B2 (ja) 2016-12-21

Similar Documents

Publication Publication Date Title
KR101257330B1 (ko) 펩타이드 정제 방법
CN103080128B (zh) Glp-1类似物的反相hplc纯化
Rodríguez et al. Design and implementation of a high yield production system for recombinant expression of peptides
JP4975895B2 (ja) 高圧液体クロマトグラフィーによるインシュリンの単離方法
KR20160120788A (ko) 신규 prongf 돌연변이체 및 베타-ngf의 제조에서의 이의 용도
KR20100053547A (ko) 재조합 인간 부갑상선 호르몬(ｒｈｐｔｈ)(1-34)의 정제용 신규한 직교 공정
US20220372074A1 (en) Production and Purification Method for Polypeptide
CN111511756A (zh) 使用烷基糖苷类进行蛋白质纯化和病毒灭活
IL248293A (en) Gonadotropin purification process
CN105473610A (zh) 用于pth的纯化方法
JP2022023814A (ja) 組換え生産された三量体型のｒｓｖタンパク質の精製方法
IL151308A (en) Cleansing of hCG and pharmaceutical preparations containing it
Ueda et al. Ni (II)-based immobilized metal ion affinity chromatography of recombinant human prolactin from periplasmic Escherichia coli extracts
ES2612427T3 (es) Método para purificar teriparatida (PTH1-34)
WO2013183948A1 (ko) 고당화된 지속형 인간 성장호르몬 단백질 및 이의 제조방법
WO2020234742A1 (en) Granulocyte colony stimulating factor purification
US9975941B2 (en) Method for purifying teriparatide
WO2019077432A1 (en) IMPROVED PURIFICATION METHOD OF RECOMBINANT PTH (1-34)
WO2014009515A1 (en) Method for producing a recombinant protein of interest
CN110582510B (zh) 用于纯化重组抗体片段的方法
CN110563832A (zh) 一种高纯度重组促卵泡刺激素纯化方法
WO1999061474A1 (fr) Activine humaine purifiee et son procede de production
JPH04299990A (ja) モチリンアナログの精製方法
KR20030010536A (ko) 융합단백질로부터 목적 단백질을 분리하는 방법
EP3429612A1 (en) Methods for producing peptides and uses thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1223378

Country of ref document: HK

RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20160406

REG Reference to a national code

Ref country code: HK

Ref legal event code: WD

Ref document number: 1223378

Country of ref document: HK