CN105466914B - A kind of high performance bioluminescent detection device - Google Patents
A kind of high performance bioluminescent detection device Download PDFInfo
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- CN105466914B CN105466914B CN201510823489.7A CN201510823489A CN105466914B CN 105466914 B CN105466914 B CN 105466914B CN 201510823489 A CN201510823489 A CN 201510823489A CN 105466914 B CN105466914 B CN 105466914B
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Abstract
The present invention provides a kind of high performance bioluminescent detection device, wherein：On the inner wall of the lid, detection storehouse inner wall on and swab support outer wall and end be both provided with one layer of shielded layer, window shielded layer is provided on the detection window, the shielding cavity that shielded layer and window shielded layer form a closing shields swab in shielding cavity；Antireflection layer is both provided on two faces of the window shielded layer.One layer of reflecting layer is both provided on the swab storehouse and the inner wall of swab support.The relatively traditional bioluminescent detection device of detection device of the invention, the accuracy and consistency of detected value improve at least 5 times or more, and detection sensitivity improves at least 10 times, and testing result is more accurate.In the case of identical light emitting diode, the present invention can at least improve 1 order of magnitude than the test result of conventional apparatus, and test value is more stable accurate, be suitable for requiring sanitary condition high scene, ensure public safety and food and environment measuring.
Description
Technical field
The invention belongs to field of biological detection, are related to bioluminescent detection, and in particular to a kind of high performance bioluminescence
Detection device.
Background technology
Bioluminescence technique is having proven to be a kind of effective, multi-functional biological analysis tool many years ago, fits
For the application of a variety of occasions.Now, in many technical fields, bioluminescence technique basic detection outstanding as one
Principle is applied.Certainly, bioluminescence technique can also be directed to the various microorganisms such as various medicine, pharmacy, food and be surpassed
Sensitive Detection.Bioluminescence is one kind of bioluminescence, and bioluminescence is applied to life science by scientist.With change
It learns fluorescence (fluorescence) to compare, bioluminescence (bioluminescence) is sensitiveer, more credible, effect is more preferable.Each life
Long-term microorganism has the ATP contents compared with constant level, and after microorganism death, ATP will be broken off quickly, therefore, extraction
The ATP of bacterium, after testing out ATP contents using biloluminescence method, you can extrapolate the bacteria containing amount in sample, whole process is only
A few minutes.Since biloluminescence method need not cultivate microbial process, easy to operate, high sensitivity is i.e. available in a short time
Testing result has the unrivaled advantage of other microorganism detection methods, is one of most fast method of current detection microorganism.
But along with these it is notable the advantages of, bioluminescence technique application when also have some shortcomings.For example, this
Invent mention how to allow the maximum amount of collection faint light of optical detecting sensor, avoid the scattering and refraction of light, avoid outer
Bound pair circuit influences.We are in past following period of time, always dependent on photodiode performance and circuit optimization or by soft
Part is realized.Optimal way mentioned above, without fundamentally solving the problems, such as one：In reagent and microbial reaction process
In, faint light is generated, faint light is absorbed by optical detecting sensor and is converted to current signal, and optical detecting sensor is to faint
The absorption of light also has certain range, so, it can be there are the scattering of part faint light or the interference in the external world, and lead to detection spirit
The reduction of sensitivity.
Invention content
In view of the deficiencies of the prior art, the present invention intends to provide a kind of high performance bioluminescent detection dress
It puts, solves existing bioluminescent detection device and be difficult to the technical issues of accurately being detected that shine to weak biological.
To solve the above-mentioned problems, the present invention is realised by adopting the following technical scheme：
A kind of high performance bioluminescent detection device, the housing including carrying lid, the interior setting detection storehouse of housing, detection
Supporting block in storehouse is installed, the swab storehouse for placing swab is machined in supporting block, swab support is placed on swab storehouse；Support
The detection window connected with swab storehouse is machined on block, pcb board is installed in the detection storehouse of detection window, is equipped on pcb board
The optical detecting sensor of alignment detection window；
On the inner wall of the lid, detection storehouse inner wall on and swab support outer wall and end be both provided with one layer of shielding
Layer, window shielded layer is provided on the detection window, the shielding cavity that shielded layer and window shielded layer form a closing will be wiped
Son shielding is in shielding cavity；
Antireflection layer is both provided on two faces of the window shielded layer.
The present invention also has following distinguishing feature：
One layer of reflecting layer is both provided on the swab storehouse and the inner wall of swab support.
The reflecting layer is used with dielectric film silver reflectance coating.
The thickness in the reflecting layer is 0.5mm.
The shielded layer uses yellow gold plated film.
The thickness of the shielded layer is 20~25 microns.
The window shielded layer is made of tin indium oxide.
The thickness of the window shielded layer is less than or equal to 0.2mm.
The antireflection layer is made of calcirm-fluoride.
The thickness of the antireflection layer is the integral multiple of 1/4 wavelength of detection light that optical detecting sensor receives, i.e.,
Follow 1/4 wavelength.
Compared with prior art, the present invention it has the following technical effect that：
The detection device of the present invention is by being electromagnetically shielded the accuracy of detection to improve faint light, by faint light to the full extent
It is absorbed by optical detecting sensor, by conversion, final output numeric results show the degree of cleanliness factor.The present invention is realizing
Swab comprehensively electromagnetic shielding on the basis of, by antireflection layer solve detection window light transmittance problem, realize electromagnetic shielding with
The compatibility of light transmittance.
The detection device of the present invention also improves the accuracy of detection of faint light by reflecting layer, and faint light is not by external environment
Interference, by the light transmission of the optically focused and antireflection layer in reflecting layer, i.e. an optically focused and the primary joint for increasing light transmission make bioluminescence
It can be absorbed entirely by optical detecting sensor substantially.
The relatively traditional bioluminescent detection device of detection device of the invention, the accuracy and consistency of detected value improve
At least 5 times or more, detection sensitivity improves at least 10 times, and testing result is more accurate.In the situation of identical light emitting diode
Under, the present invention can at least improve 1 order of magnitude than the test result of conventional apparatus, and test value is more stable accurate, be suitable for pair
Sanitary condition requires high scene, ensures public safety and food and environment measuring.
Description of the drawings
Fig. 1 is the structure diagram in the detection storehouse of the present invention.
Fig. 2 is the overall structure diagram of the present invention.
Fig. 3 is the front and rear test result comparison diagram using the present invention.
The meaning of each label is in figure：1- housings, 2- detections storehouse, 3- supporting blocks, 4- swabs, 5- swabs storehouse, 6- swabs
Support, 7- detection windows, 8-PCB plates, 9- optical detecting sensors, 10- shielded layers, 11- window shielded layers, 12- antireflection layers, 13- lids
Son, 14- reflecting layer.
Explanation is explained in detail to the particular content of the present invention below in conjunction with drawings and examples.
Specific embodiment
Specific embodiments of the present invention are given below, it should be noted that the invention is not limited in implement in detail below
Example, all equivalents done on the basis of technical scheme each fall within protection scope of the present invention.
Embodiment：
Above-mentioned technical proposal is deferred to, as depicted in figs. 1 and 2, this gives a kind of high performance bioluminescences to examine
Device, the housing 1 including carrying lid 13 are surveyed, the interior setting detection storehouse 2 of housing 1 is detected in storehouse 2 and is equipped with supporting block 3, supporting block
The swab storehouse 5 for placing swab 4 is machined on 3, swab support 6 is placed on swab storehouse 5；It is machined in supporting block 3 and swab
The detection window 7 that storehouse 5 connects is equipped with pcb board 8 in the detection storehouse 2 of detection window 7, and alignment detection window 7 is equipped on pcb board 8
Optical detecting sensor 9；
On the inner wall of the lid 13, detection storehouse 2 inner wall on and swab support 6 outer wall and end be both provided with one layer
Shielded layer 10, window shielded layer 11 is provided on the detection window 7, and shielded layer 10 and window shielded layer 11 form a closing
Shielding cavity swab 4 is shielded in shielding cavity；
Be both provided with antireflection layer 12 on two faces of the window shielded layer 11 so that photon percent of pass reach 99% with
On.
One layer of reflecting layer 14 is both provided on the inner wall of swab storehouse 5 and swab support 6, reflecting layer increases the optically focused in swab 4,
Reflecting layer 14 prevents from aoxidizing using silver-colored reflectance coating, and material, which is protected, using dielectric film is made, which is high reflection
Property, more than 95% is reflected up to light, thickness 0.5mm or so.After increasing reflecting layer, greatly improve the collection efficiency of light.
For shielded layer 11 using silver-bearing copper metallic composition, the thickness of shielded layer 11 is 20-35 microns, uniform to instrument internal
Increase shielded layer, faint light detection is made testing result will not to be made more accurate, unanimously by external electromagnetic, the interference of noise.
Window shielded layer 12 is made of indium tin oxide material, and thickness is within 0.2mm.Window shielded layer 12 must be led
Electric material, while there are the interference performances such as shielding electromagnetism, signal.
Antireflection layer 13 is made of calcium fluoride material, and thickness follows 1/4 wavelength, by increasing this antireflection layer, makes faint light
Percent of pass increase, allow light percent of pass maximum to the full extent, loss will arrive minimum.
Performance test：
After cotton swab to be checked is put into swab 4, reaction solution is squeezed into test tube, liquid has weak biological hair in test tube
Light.Faint light first passes through reflecting layer 14, by light without refraction, reflection enhancement, the increase of ray-collecting rate；Meanwhile 10 He of shielded layer
Other interference shieldings such as electromagnetism can be made test value will not be by ectocine by window shielded layer 11.After this secondary reflection, light
After converging to antireflection layer 12 and window shielded layer 11 across detection window 7, make that light stablized it is anti-reflection after, all by optical detection
Sensor 9 is absorbed, and is converted to current signal, then turns potential circuit through overcurrent, and using filter circuit, high frequency is believed
It number filters out, is finally converted by A/D, export the test result of digital quantity, convert the result to bacteria total amount, to determine whether
Reach HACCP and health test.
The performance that traditional bioluminescent detection device and the bioluminescent detection device of the present invention will be compared below, wherein
Traditional bioluminescent detection device is labeled as old, and the bioluminescent detection device of the present invention is labeled as new.
Table 1 is Sensitivity comparison table, from table 1 it follows that traditional bioluminescent detection device and the life of the present invention
There are the differences on the order of magnitude for the sensitivity of object luminescence detection apparatus.
Table 2 is repeated statistical result, and the repeatability of new instrument is obviously improved, the test result of all similar concentration levels
CV is substantially reduced, and especially in low concentration level, is improved and is protruded.
Table 3 is linear statistical as a result, with r being standard more than 0.990, and the range of linearity of new instrument expands downwards one
The order of magnitude.
Table 4 is to improve front and rear test data, from table 4, it can be seen that using the technology referred in patent, sensitivity improves
An order of magnitude, repeatability improve 8 times or so.
Fig. 3 is the linear related coefficient of the detection of instrument, from figure 3, it can be seen that after treatment, the detection of instrument is linear
Related coefficient be 1.
By experimental results demonstrate using the detection device of the present invention, relatively traditional bioluminescent detection device uses
The detection device, detection sensitivity improve at least 10 times, and testing result is more accurate, and stability is high.
1 Sensitivity comparison table of table
| The detection of old instrument limits | 1E-16 |
| The detection of new instrument is limited to | 5E-17 |
The repeated statistical result of table 2
| Concentration | CVold | Cvnew |
| 5E-17 | It can not detect | 36.89% |
| 1E-16 | 51.60% | 7.03% |
| 5E-16 | 32.08% | 4.89% |
| 5E-15 | 7.42% | 5.35% |
| 5E-14 | 7.02% | 4.71% |
| 5E-13 | 6.13% | 3.41% |
3 linear statistical result of table
| old | 1E-16~5E-13 |
| new | 5E-17~5E-13 |
Table 4 improves front and rear test data
Claims (1)
1. a kind of high performance bioluminescent detection device, the housing (1) including carrying lid (13), the interior setting inspection of housing (1)
It surveys storehouse (2), supporting block (3) is installed in detection storehouse (2), the swab storehouse for placing swab (4) is machined in supporting block (3)
(5), it is placed with swab support (6) on swab storehouse (5)；The detection window (7) connected with swab storehouse (5) is machined in supporting block (3), is leaned on
Pcb board (8) is installed in the detection storehouse (2) of nearly detection window (7), the optics inspection of alignment detection window (7) is installed on pcb board (8)
Survey sensor (9), it is characterised in that：
On the inner wall of the lid (13), on the inner wall of detection storehouse (2) one is both provided with the outer wall of swab support (6) and end
Layer shielded layer (10) is provided with window shielded layer (11), shielded layer (10) and window shielded layer (11) on the detection window (7)
The shielding cavity for forming a closing shields swab (4) in shielding cavity；
The shielded layer (10) is using yellow gold plated film；The thickness of the shielded layer (10) is 20~25 microns；
The window shielded layer (11) is made of tin indium oxide；The thickness of the window shielded layer (11) is less than or equal to
0.2mm；
Antireflection layer (12) is both provided on two faces of the window shielded layer (11)；The antireflection layer (12) is using fluorination
Calcium is made；The thickness of the antireflection layer (12) is the integer of 1/4 wavelength of detection light that optical detecting sensor (9) receives
Times；
One layer of reflecting layer (14) is both provided on the swab storehouse (5) and the inner wall of swab support (6)；The reflecting layer (14)
Using with dielectric film silver reflectance coating；The thickness in the reflecting layer (14) is 0.5mm.
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| CN201510823489.7A CN105466914B (en) | 2015-11-24 | 2015-11-24 | A kind of high performance bioluminescent detection device |
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| CN201510823489.7A CN105466914B (en) | 2015-11-24 | 2015-11-24 | A kind of high performance bioluminescent detection device |
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| CN105466914A CN105466914A (en) | 2016-04-06 |
| CN105466914B true CN105466914B (en) | 2018-07-03 |
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| CN116840493B (en) * | 2023-08-31 | 2023-11-28 | 北京白象新技术有限公司 | Detection device and detection method for cleaning residual protein |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| GB0324784D0 (en) * | 2003-10-24 | 2003-11-26 | Biotrace Ltd | Bioluminescence monitor |
| CN201034925Y (en) * | 2007-04-03 | 2008-03-12 | 西安天隆科技有限公司 | Hand-hold adenosine triphosphate fluorescent testing apparatus |
| CN101995481B (en) * | 2009-08-26 | 2012-09-26 | 中国科学院电子学研究所 | Fast detector for ultra-trace disease markers |
| CN202837186U (en) * | 2012-09-24 | 2013-03-27 | 宁波高新区绿邦科技发展有限公司 | Sampling detecting device of bioluminescence detector |
| CN203720091U (en) * | 2014-02-26 | 2014-07-16 | 广州博鹭腾仪器仪表有限公司 | Biological weak light signal detection circuit |
| CN204405547U (en) * | 2015-03-12 | 2015-06-17 | 北京赛必达科技有限公司 | Novel portable ATP fluorescence detector |
| CN205175914U (en) * | 2015-11-24 | 2016-04-20 | 西安天隆科技有限公司 | High -performance bioluminescence detection device |
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