CN105384840A - Method for extracting lentinula edodes foot polysaccharides - Google Patents
Method for extracting lentinula edodes foot polysaccharides Download PDFInfo
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- CN105384840A CN105384840A CN201510976711.7A CN201510976711A CN105384840A CN 105384840 A CN105384840 A CN 105384840A CN 201510976711 A CN201510976711 A CN 201510976711A CN 105384840 A CN105384840 A CN 105384840A
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- lentinula edodes
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The invention relates to a method for extracting lentinula edodes foot polysaccharides. The method includes the steps that mildew-free insect-free odor-free lentinula edodes feet are selected, aging parts at the lower ends of the lentinula edodes feet are cut off, the lentinula edodes feet are cleaned, placed in an oven and dried at the temperature of 100-105 DEG C till the water content is 3-5%, and the lentinula edodes feet are smashed to 200-300 meshes; the obtained powder is added into distilled water with the volume 20-25 times that of the powder, neutral proteinase, cellulase and pectinase are added and extracted for 60-180 min under the condition that the temperature is 45-55 DEG C and the pH value is 5-6, the mixture is stirred at intervals and filtered, collected extraction liquid is concentrated till the volume of the extracted liquid is 1/3-1/2 that of original liquid, and concentrated liquid is obtained; the obtained concentrated liquid is added into a 90-95% ethanol solution with the volume 4-5 times that of the concentrated liquid to be precipitated for 12-24 h, centrifugal separation and precipitation are carried out, the filtered liquid is dried in a vacuum mode, and the lentinula edodes foot polysaccharides are obtained. The method mainly has the advantages that by the adoption of the method, the solubility rate of the polysaccharides is increased, conditions are moderate, operation is easy, cost is low, the activity of a target product is high, and the extraction rate and the purity of the polysaccharides are high.
Description
(1) technical field
The present invention relates to a kind of extracting method of mushroom pin polysaccharide.
(2) background technology
Mushroom is Chinese Famous edible mushrooms, at the title have " mountain delicacy " among the people.Mushroom contains the antineoplastic component that a kind of molecular weight is 1,000,000---and lentinan, modern study proves, the adjustable people Xiu Nei of lentinan has the T cell of immunologic function active, can reduce the ability of methyl-cholanthrene induced tumor.
Mushroom pin is the handle of mushroom, is the dry product that the stem section of mushroom is dried.Adopt Hot water extraction or organic solvent extraction to the extraction of mushroom pin polysaccharide at present, the former easily causes active polysaccharide to reduce more, and energy consumption is large, yield low (lower than 3%); There is the problems such as solvent use cost is high, recovery difficulty, product dissolvent residual in latter, is therefore necessary to make improvements.
(3) summary of the invention
The present invention seeks to for of the prior art above not enough, provide that a kind of cost is low, method is simple, extraction yield is high, the extracting method of the mushroom pin polysaccharide of good product quality.
The technical solution used in the present invention is:
An extracting method for mushroom pin polysaccharide, described method comprises:
(1) select without going mouldy, free from insect pests, free from extraneous odour mushroom pin, cut off the aging part in mushroom handle lower end, cleaning and being placed in baking oven that 100 ~ 105 DEG C are dried to water content is 3 ~ 5% (w/w), micronizing to 200 ~ 300 order;
(2) step (1) gained powder adds in the distilled water of 20 ~ 25 times of volumes (every 1g powder adds 20 ~ 25mL distilled water), add neutral protease, cellulase and polygalacturonase, in 45 ~ 55 DEG C, lixiviate 60 ~ 180 minutes under the condition of pH5 ~ 6, gap is stirred, filter, collection extracting solution is concentrated into 1/3 ~ 1/2 of original volume and obtains concentrated solution; Described neutral protease quality consumption is 2 ~ 3% (enzyme quality accounts for mushroom pin powder quality per-cent, lower same), and cellulase quality consumption is 1 ~ 2%, and polygalacturonase quality consumption is 1 ~ 2%; Neutral protease used in the present invention is purchased from upper sea blue season development in science and technology company limited, and unit enzyme is lived 40U/mg; Cellulase is purchased from Bai Ao bio tech ltd, Shanghai, and unit enzyme is lived 15U/mg; Polygalacturonase is purchased from upper sea blue season development in science and technology company limited, and unit enzyme is lived 30U/mg.
(3) step (2) gained concentrated solution adds 90 ~ 95% ethanolic solns precipitation 12 ~ 24 hours of 4 ~ 5 times of volumes, and centrifugation precipitates, and filter vacuum is dry, obtains mushroom pin Crude polysaccharides.
The present invention is first under the effect of micronizing machinery, destroy the cell walls of mushroom pin, release intracellular polyse, then employing prozyme acts synergistically on the protein digestion that polysaccharide combines by leaching process prozyme, reduces the content of protein while the extraction yield improving polysaccharide.The method not only increases productive rate, improves the purity of polysaccharide simultaneously, simplifies the purification work of polysaccharide, greatly increases economic benefit.
Preferably, in step (2), each enzyme dosage is: neutral protease quality consumption is 2%, and cellulase quality consumption is 1%, and polygalacturonase quality consumption is 1%.
Preferably, in step (2), spray drying parameters is as follows: inlet temperature controls, at 180 DEG C ~ 220 DEG C, to be regulated by control heater; Air outlet temperature controls, at 80 DEG C ~ 120 DEG C, to be regulated by the input speed of material.
Beneficial effect of the present invention is mainly reflected in: adopt the inventive method, improve the solubility rate of polysaccharide, mild condition, simple to operate, cost is low, and target product activity is high, and the extraction yield of polysaccharide is high, quality good.
(4) embodiment
Below in conjunction with specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in this:
Neutral protease used in embodiment is purchased from upper sea blue season development in science and technology company limited, and unit enzyme is lived 40U/mg; Cellulase is purchased from Bai Ao bio tech ltd, Shanghai, and unit enzyme is lived 15U/mg; Polygalacturonase is purchased from upper sea blue season development in science and technology company limited, and unit enzyme is lived 30U/mg.
Embodiment 1:
Select without going mouldy, free from insect pests, free from extraneous odour mushroom pin, cut off the aging part in mushroom handle lower end, get 100kg mushroom pin, oven dry to its moisture content is 2%, first coarse reduction (crossing 20 orders), then micronizing (crossing 300 orders), gained powder adds in 25 times of volume distilled water and adds by neutral protease 2%, the prozyme of cellulase 1.0%, polygalacturonase 1.0% composition, lixiviate 60 minutes under temperature 55 DEG C, pH6 condition.Gap is stirred, filter, collecting concentrated (-0.06Mpa ~-0.08Mpa concentrating under reduced pressure) to the relative density of extracting solution is 1.03, get the 90% alcohol settling 18h that concentrated solution adds 4 times of volumes, centrifugation precipitates, obtain mushroom pin Crude polysaccharides 8.5 kilograms after filtrate spraying dry (inlet temperature controls at 180 DEG C ~ 200 DEG C, and air outlet temperature controls at 100 DEG C ~ 120 DEG C), extraction yield is 8.5%.
Embodiment 2:
Select without going mouldy, free from insect pests, free from extraneous odour mushroom pin, cut off the aging part in mushroom handle lower end, get 100kg mushroom pin, oven dry to its moisture content is 3%, first coarse reduction (crossing 20 orders), then micronizing (crossing 300 orders), gained powder adds in 20 times of volume distilled water and adds by neutral protease 2%, the prozyme of cellulase 1.0%, polygalacturonase 1.0% composition, lixiviate 120 minutes under temperature 55 DEG C, pH5 condition.Gap is stirred, filter, collecting concentrated (-0.06Mpa ~-0.08Mpa concentrating under reduced pressure) to the relative density of extracting solution is 1.03, get the 95% alcohol settling 18h that concentrated solution adds 4 times of volumes, centrifugation precipitates, obtain mushroom pin Crude polysaccharides 9.3 kilograms after filtrate spraying dry (inlet temperature controls at 180 DEG C ~ 200 DEG C, and air outlet temperature controls at 80 DEG C ~ 100 DEG C), extraction yield is 9.0%.
Embodiment 3:
Select without going mouldy, free from insect pests, free from extraneous odour mushroom pin, cut off the aging part in mushroom handle lower end, get 100kg mushroom pin, oven dry to its moisture content is 3%, first coarse reduction (crossing 20 orders), then micronizing (crossing 300 orders), gained powder adds in 20 times of volume distilled water and adds by neutral protease 2%, the prozyme of cellulase 1.0%, polygalacturonase 1.0% composition, lixiviate 180 minutes under temperature 50 C, pH5 condition.Gap is stirred, filter, collecting concentrated (-0.06Mpa ~-0.08Mpa concentrating under reduced pressure) to the relative density of extracting solution is 1.04, get the 95% alcohol settling 24h that concentrated solution adds 4 times of volumes, centrifugation precipitates, obtain mushroom pin Crude polysaccharides 9.8 kilograms after filtrate spraying dry (inlet temperature controls at 200 DEG C ~ 220 DEG C, and air outlet temperature controls at 120 DEG C ~ 120 DEG C), extraction yield is 9.8%.
Claims (3)
1. an extracting method for mushroom pin polysaccharide, described method comprises:
(1) select without going mouldy, free from insect pests, free from extraneous odour mushroom pin, cut off the aging part in mushroom handle lower end, cleaning and being placed in baking oven that 100 ~ 105 DEG C are dried to water content is 3 ~ 5%, is crushed to 200 ~ 300 orders;
(2) step (1) gained powder adds in the distilled water of 20 ~ 25 times of volumes, add neutral protease, cellulase and polygalacturonase, in 45 ~ 55 DEG C, lixiviate 60 ~ 180 minutes under the condition of pH5 ~ 6, gap is stirred, filter, it is 1.03 ~ 1.05 that collection extracting solution is concentrated into relative density, obtains concentrated solution; Described neutral protease quality consumption is 2 ~ 3%, and cellulase quality consumption is 1 ~ 2%, and polygalacturonase quality consumption is 1 ~ 2%;
(3) step (2) gained concentrated solution add 4 ~ 5 times of volumes 90 ~ 95% ethanolic solns precipitation 12 ~ 24 hours, centrifugation precipitate, filtrate spraying dry, obtains mushroom pin Crude polysaccharides.
2. the method for claim 1, is characterized in that in step (2), each enzyme dosage is: neutral protease quality consumption is 2%, and cellulase quality consumption is 1%, and polygalacturonase quality consumption is 1%.
3. the method for claim 1, is characterized in that in step (3), spray drying parameters is as follows: inlet temperature controls at 180 DEG C ~ 220 DEG C, and air outlet temperature controls at 80 DEG C ~ 120 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510976711.7A CN105384840A (en) | 2015-12-22 | 2015-12-22 | Method for extracting lentinula edodes foot polysaccharides |
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| CN201510976711.7A CN105384840A (en) | 2015-12-22 | 2015-12-22 | Method for extracting lentinula edodes foot polysaccharides |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106749751A (en) * | 2017-01-16 | 2017-05-31 | 天津大学 | A kind of lentinan with the effect of anti-liver cancer and anti-enhance immunity and preparation method thereof |
| CN106835775A (en) * | 2016-12-23 | 2017-06-13 | 句容市申兔工艺针织厂 | A kind of preparation method of plant color fixing agent |
| CN107349240A (en) * | 2017-06-23 | 2017-11-17 | 百色学院 | A kind of extracting method of mangrove bark polyphenol |
Citations (4)
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| CN1765935A (en) * | 2005-11-04 | 2006-05-03 | 杨勇杰 | Method for separating lentinan |
| CN101555292A (en) * | 2009-05-26 | 2009-10-14 | 何颖娜 | Method for extracting lentinan from foot of lentinus edodes |
| CN101906172A (en) * | 2010-07-22 | 2010-12-08 | 吴茂玉 | Method for extracting water-insoluble polysaccharide of mushroom by enzyme process |
| CN104861079A (en) * | 2015-04-27 | 2015-08-26 | 浙江山狼谷旅游产业发展有限公司 | Microwave assisted lentinan extraction equipment and technology |
-
2015
- 2015-12-22 CN CN201510976711.7A patent/CN105384840A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1765935A (en) * | 2005-11-04 | 2006-05-03 | 杨勇杰 | Method for separating lentinan |
| CN101555292A (en) * | 2009-05-26 | 2009-10-14 | 何颖娜 | Method for extracting lentinan from foot of lentinus edodes |
| CN101906172A (en) * | 2010-07-22 | 2010-12-08 | 吴茂玉 | Method for extracting water-insoluble polysaccharide of mushroom by enzyme process |
| CN104861079A (en) * | 2015-04-27 | 2015-08-26 | 浙江山狼谷旅游产业发展有限公司 | Microwave assisted lentinan extraction equipment and technology |
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| 余冬生: "酶法提取香菇多糖", 《江苏食品与发酵》 * |
| 李红卫等: "从香菇中浸提香菇多糖的方法对比研究", 《食品科学》 * |
| 王挥等: "生物酶法在香菇多搪提取中的应用研究", 《湖北省药学会第十一届会员代表大会暨2007年学术年会论文汇编》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106835775A (en) * | 2016-12-23 | 2017-06-13 | 句容市申兔工艺针织厂 | A kind of preparation method of plant color fixing agent |
| CN106749751A (en) * | 2017-01-16 | 2017-05-31 | 天津大学 | A kind of lentinan with the effect of anti-liver cancer and anti-enhance immunity and preparation method thereof |
| CN107349240A (en) * | 2017-06-23 | 2017-11-17 | 百色学院 | A kind of extracting method of mangrove bark polyphenol |
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