CN105310796B - An artificial blood vessel and its preparation method and needling mold - Google Patents

An artificial blood vessel and its preparation method and needling mold Download PDF

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CN105310796B
CN105310796B CN 201410294238 CN201410294238A CN105310796B CN 105310796 B CN105310796 B CN 105310796B CN 201410294238 CN201410294238 CN 201410294238 CN 201410294238 A CN201410294238 A CN 201410294238A CN 105310796 B CN105310796 B CN 105310796B
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artificial blood
blood vessel
spinning
method
preparing
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CN105310796A (en )
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陈大凯
杜广武
丁峰
罗七
罗七一
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上海微创医疗器械(集团)有限公司
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Abstract

本发明公开了一种人造血管及其制备方法和针刺模具,所述制备方法包括如下步骤:裁剪织物并将织物缝合成圆筒形,形成血管内层管壁;将所述内层管壁套设在金属棒上,将金属棒作为静电纺丝接收端,进行静电纺丝,使得纳米纤维均匀粘附在内层管壁的外表面;当纳米纤维达到预设厚度后,停止纺丝,形成血管外层管壁,从所述金属棒上取下人造血管;将人造血管套在弹性棒上,对人造血管进穿刺,使外面的纺丝层和内层牢固结合。 The present invention discloses a method for preparing an artificial blood vessel and the needle and the mold, said process comprising the steps of: cutting the fabric and the fabric was sewn into a cylindrical shape, the inner wall of a vessel; and the inner tube wall sleeved on the metal rod, a metal rod receiving end as electrostatic spinning, electrostatic spinning, such that the nanofibers uniformly adhered on the outer surface of the inner tube wall; when nanofiber reaches a predetermined thickness, the spinning is stopped, forming the outer vessel wall, an artificial blood vessel is removed from the metal bar; artificial blood tubing on the elastic rod, to an artificial blood vessel into the puncture, the spinning outer and inner layers of strongly bonded. 本发明提供的人造血管能够提高人造血管的抗渗透能力和机械强度,具有抗形变及高耐久性特点。 The present invention provides an artificial blood vessel can be improved and the mechanical strength of the anti-penetration of artificial blood vessels, high durability and deformation resistant characteristics.

Description

一种人造血管及其制备方法和针刺模具 An artificial blood vessel and its preparation method and needling mold

技术领域 FIELD

[0001] 本发明涉及一种植入性医疗器械,尤其涉及一种人造血管及其制备方法和穿刺模具。 [0001] The present invention relates to implantable medical devices, and particularly to a method for preparing an artificial blood vessel and a piercing die.

背景技术 Background technique

[0002] 目前,在全世界,心血管疾病及周围动脉疾病是引起人类死亡的主要原因。 [0002] Currently, in the world, cardiovascular disease and peripheral arterial disease is the leading cause of death. 每年大约需要实施600000例血管移植手术,但是接近三分之一的病患者因为血管老化,血管病变、 损伤或之前手术使用等原因而无法使用自体血管。 It takes about 600,000 cases each year of vascular graft surgery, but nearly one-third of patients because of vascular aging, vascular disease, and other causes injury or surgery can not be used prior to autologous blood vessels. 在这种情况下,使用人工血管成为了很好的选择。 In this case, the use of artificial blood vessels become a good choice. 20世纪50年代,自从人们观察到细胞在浸入血液的丝线上生长的现象后,就开始了纺织型人造血管的制造历史。 In the 1950s, since the phenomenon has been observed in cells grown thread immersed in blood, began the history of textile manufacture of artificial blood vessels. 时至今日,纺织型人造血管已广泛地应用于临床,替换人体内已坏损的血管,并在临床上取得了较好的医疗效果。 Today, the textile type vascular prostheses have been widely used in clinical, replace the body has been corrupted blood vessels and achieved good clinical medical effects. 但同时,纺织型人造血管也面临着更多的挑战。 But at the same time, the textile-type artificial blood vessel is also facing more challenges.

[0003] 静电纺丝技术是近年来兴起的一种制备纳米至微米级纤维的十分有效的方法。 [0003] The electrospinning technique is a very effective method of preparing a nano to micron-sized in recent years the rise of the fibers. 静电纺丝技术是指在高压电场的条件下,使高分子纺丝溶液形成点点的喷射流,喷射流中的溶剂在到达接收装置之前挥发,带电的高分子液滴克服表面张力瞬间被拉伸上千万倍,最后在接收装置上形成纤维网状结构的纳米纤维。 Electrostatic spinning technology is at a high voltage electric field, the polymer spinning solution formed a little jet, jet solvent is volatilized before reaching the receiving apparatus, the charged droplets of polymer to overcome the surface tension is instantaneously stretched million times, and finally the formation of fibrous network of nanofibers on the receiving device. 这种技术设备简易,成本低廉,制备出的材料具有较高的比表面积,可控纤维的直径及孔隙率,可以制备出接近天然细胞外基质尺度范围的纤维。 This technique is simple equipment, low cost, the material prepared has a high specific surface area, porosity and controllable diameter fibers can be prepared close to the natural extracellular matrix scales fibers. 大量研宄表明,细胞在小于自身尺度的纳米纤维支架上具有更好的粘附、增殖和迁移特性。 Large study based show better adhesion of cells on itself is less than the dimension of the nanofiber scaffolds, proliferation and migration properties. 因此利用静电纺丝技术制备人工血管成为一个热点。 Thus prepared using electrospinning graft become a hot spot.

[0004] 现有技术中公开了一种通过静电纺丝制备人工血管的方法:将高分子溶液静电纺到高速旋转的接收器上,得到取向纤维膜;然后将取向纤维膜绕金属模具卷成管状,纤维取向方向和模具长轴方向相同;再次进行静电纺丝,用套有取向纤维膜的金属模具接收;得到了两层结构的人造血管。 [0004] The prior art discloses a process for preparing graft by electrostatic spinning: The polymer solution was spun electrostatically to the receiver high-speed rotation, an oriented fiber membrane; fiber alignment film was then wound into a roll mold tubular, the same fiber orientation direction and the axial direction of the mold; electrospinning again, jacket alignment with the dies receiving fiber membranes; artificial blood vessel obtained two-layer structure. 该方法需要经过多次静电纺丝,工艺复杂;现有技术还公开了一种在机织、针织或编织结构组成的外层和纳米纤维组成的内层的双层结构人造血管。 The electrospinning process requires many times, complicated process; prior art also discloses a two-layer structure A woven, knitted or braided structure composed of an outer layer and an inner layer of nanofibers artificial blood vessel. 其中纳米纤维层用静电纺丝制备,纺丝完成后,将收集有纳米纤维的表面翻转到内层,构成人造血管的内表面,但是翻转容易损坏血管。 Wherein the nanofiber layer is prepared by electrospinning, after spinning is completed, the collected nanofiber surface to flip the inner layer constituting the inner surface of the artificial blood vessel, but the flip easily damaged blood vessels.

[0005] 目前现有的人造血管中存在的重要问题是织物的抗渗透能力仍然比较差,且机械强度不够。 [0005] It's important problems of the prior artificial vessel present is an anti-permeability of the fabric is still relatively poor, and the mechanical strength is insufficient. 理想的人造血管管壁应具有合适尺寸的孔隙,既有利于细胞组织的长入,又不能因为孔隙的存在而导致手术过程中的大量渗血。 Over the artificial blood vessel wall should have a suitable pore size, conducive to tissue ingrowth, but not because the presence of voids caused by the large amount of bleeding during surgery. 所以,如何制成功能更全面的人造血管依然是近年来的研究热点课题。 So, how is made more comprehensive study of artificial blood vessels it is still a hot topic in recent years.

发明内容 SUMMARY

[0006] 本发明所要解决的技术问题是提供一种人造血管及其制备方法,制备的人造血管具有高耐久抗形变的性能,能够防止手术时管壁渗血,有利于手术后细胞组织的顺利长入, 同时还能提供足够的机械强度。 [0006] The present invention solves the technical problem and to provide a method for preparing an artificial blood vessel, an artificial blood vessel prepared having highly durable anti-deformation properties of the wall can be prevented when the bleeding operation, the smooth tissue after surgery in favor ingrowth, while providing sufficient mechanical strength. _ _

[0007] 本发明为解决上述技术问题而采用的技术方案是提供一种人造血管的制备方法, 包括如下步骤:裁剪织物并将织物缝合成圆筒形,形成血管内层管壁;将所述内层管壁套设在金属棒上,将金属棒作为静电纺丝接收端,进行静电纺丝,使得纳米纤维均匀粘附在所述内层管壁的外表面;当纳米纤维达到预设厚度后,停止纺丝,形成血管外层管壁;从所述金属棒上取下人造血管;将人造血管套在弹性棒上,对所述人造血管进行穿刺,使得所述外层管壁和内层管壁牢固结合。 [0007] Technical Solution The present invention to solve the above problems is to provide a use of an artificial blood vessel prepared, comprising the steps of: cutting the fabric and the fabric was sewn into a cylindrical shape, the inner wall of a vessel; the the inner wall is sleeved on the metal rod, a metal rod receiving end as electrostatic spinning, electrostatic spinning, such that the nanofibers uniformly adhered to an outer surface of said inner wall; when the thickness reaches a preset nanofiber after the spinning is stopped, the outer wall of a vessel; removing the artificial vessel from said metal rod; artificial blood tubing on the elastic rod, the artificial blood vessel puncture, so that the inner and outer wall wall layer strongly bonded.

[0008] 在上述人造血管的制备方法中,所述织物为机织或针织的织物结构。 [0008] In the production method of the artificial blood vessel, the fabric is woven or knitted fabric structure.

[0009] 在上述人造血管的制备方法中,所述织物的材料选自涤纶、氨纶、蚕丝、聚四氟乙烯中的一个或其中多个形成的混纺材料。 [0009] In the production method of the artificial blood vessel, the fabric material is selected from polyester, spandex, silk, polytetrafluoroethylene or a form in which a plurality of blended material.

[0010] 在上述人造血管的制备方法中,所述外层管壁的直径和所需人体血管直径相一致,所述外层管壁的外径范围为6mm〜38mm。 [0010] In the production method of the artificial blood vessel, the desired diameter of the outer wall of the body and the blood vessel diameter coincide, the outer diameter of the outer wall ranges 6mm~38mm.

[0011] 在上述人造血管的制备方法中,所述静电纺丝的电压为5-50kV,纺丝距离为5-50圓,纺丝速度为0.01-10ml。 [0011] In the production method of the artificial blood vessel, the electrostatic spinning, the voltage was 5-50kV, from 5 to 50 circular spinning, spinning speed was 0.01-10ml. 优选的电压为1〇〜30kV,纺丝距离为5〜30mm,纺丝速度为0.1 〜5ml/h〇 Preferred 1〇~30kV voltage, spinning distance 5~30mm as a spinning speed of 0.1 ~5ml / h〇

[0012]在上述人造血管的制备方法中,所述静电纺丝的原料选自聚氨酯、聚乳酸、聚己内酯、聚乙烯醇、洚纶、聚甲基丙烯酸甲酯、聚苯乙烯、聚烯烃、硅橡胶中的一个或其中多个的共聚物、胶原、壳聚糖、纤维素、明胶、纤维蛋白原或丝素蛋白。 [0012] In the production method of the artificial blood vessel, the electrospun material selected from polyurethane, polylactic acid, polycaprolactone, polyvinyl alcohol, flood nylon, polymethyl methacrylate, polystyrene, poly olefins, wherein one or more of the copolymers of silicone rubber, collagen, chitosan, cellulose, gelatin, fibrinogen, or silk fibroin.

[0013] 在上述的人造血管的制备方法中,所述静电纺丝的原料的溶液质量浓度为2.5-20% 〇 [0013] In the above method of producing an artificial blood vessel, the electrospinning solution is 2.5 to 20 mass% concentration of the feedstock billion

[0014]在上述人造血管的制备方法中,所述穿刺方法为针刺或水刺。 [0014] In the production method of the artificial blood vessel, the needle or puncture method is hydroentangled.

[0015] 在上述的人造血管的制备方法中,所述弹性棒的材料为橡胶、热塑性弹性体或泡沫塑料。 [0015] In the above method of producing an artificial blood vessel, the elastic material is a rubber rod, a thermoplastic elastomer or foam.

[0016] 本发明为解决上述技术问题还提供一种人造血管,通过以上方法制备得到。 [0016] The present invention is to solve the above technical problem is also to provide a vascular prosthesis, prepared by the above method.

[0017] 本发明为解决上述技术问题还提供了一种用于上述穿刺步骤的针刺模具,其具有环状主体及多个刺针,所述环状主体具有中心轴线并且所述环状主体沿着所述中心轴线纵向延伸,所述环状主体具有靠近所述中心轴线的内表面及远离所述中心轴线的外表面,所述这些刺针均匀布置于所述环状主体的内表面上,并指向所述中心轴线。 [0017] The present invention is to solve the aforementioned technical problem also provides a mold of the above puncturing needle for the step, which has an annular body and a plurality of needles, said annular body having a central axis and along said annular body extending longitudinally with the central axis, said annular body having an inner surface and an outer surface adjacent said central axis away from the central axis, a lancet which uniformly disposed on the inner surface of the annular body, and directed toward the central axis.

[0018] 在本发明一较佳实施例中,所述环状主体由多个弧形件组成,所述这些弧形件的半径与所述人造血管的半径相一致。 [0018] In a preferred embodiment of the present invention, the annular body consisting of a plurality of arcuate members, the radius of said arcuate member with a radius of the artificial blood vessel coincide.

[0019] 本发明对比现有技术有如下的有益效果:本发明提供的人造血管及其制备方法, 直接利用套有圆筒形织物的金属棒作为纳米纤维接收端进行静电纺丝,从而可以简单方便地控制人造血管管壁的尺寸和孔隙,将纺丝完成的人造血管套在相应直径的弹性棒上,用针刺模具对人造血管进行穿刺,能够保证穿刺均匀,使得外面的纺丝层和内层结合更牢固, 既有利于细胞组织的长入,又不会因为孔隙的存在而导致手术过程中的大量渗血,还能提高机械强度;双层管壁的人造血管直接成型,并且纳米纤维层设于圆筒形织物的外面,从而能够提高人造血管的抗渗透能力,因此,本发明提供的人造血管具有抗形变及高耐久性特点。 [0019] comparative prior art the present invention has the following advantages: an artificial blood vessel and the preparation method of the present invention provides, directly a cylindrical sleeve with a metal rod a fabric, as the receiving end nanofiber electrospinning, can be simple easily control the pore size and wall of artificial blood vessels, artificial blood vessel to complete the spinning sleeve on the respective diameters of the elastic rod, an artificial blood vessel with a puncture needle of the mold, to ensure a uniform puncturing, such that the outer layer and the spinning inner tightly bind both conducive to tissue ingrowth, but not because of the presence of voids caused by the large amount of bleeding during surgery, but also improve the mechanical strength; double-wall vascular prostheses formed directly, and the nano fiber layer is provided on the outside of the cylindrical fabric, it is possible to improve the anti-penetration of artificial blood vessels, therefore, the present invention provides a vascular prosthesis having high durability and deformation resistance characteristics.

附图说明 BRIEF DESCRIPTION

[0020] 图1为本发明的较佳实施例中的高耐久抗形变人造血管的制备流程示意图; The fabrication process of this embodiment vascular prosthesis highly durable anti-deformation schematic preferred [0020] embodiment of FIG. 1 of the present invention;

[0021]图2a、图2b及图2c为不同浓度的聚合物静电纺丝电镜照片效果图; [0021] Figures 2a, 2b and 2c is an electron micrograph electrostatic spinning of polymer renderings of different concentrations;

[0022]图3为两瓣针刺模具示意图; [0022] FIG. 3 is a schematic diagram of two needle mold;

[0023]图4为四瓣针刺模具示意图。 [0023] FIG. 4 is a schematic view of four acupuncture mold.

[0024]图中: [0024] In the drawings:

[0025] 1弧形件2刺针 [0025] 1 2 lancet arcuate member

具体实施方式 detailed description

[0026]下面结合附图对本发明的较佳实施方式作进一步的描述。 [0026] The following drawings of the preferred embodiments of the present invention will be further described in conjunction.

[0027]图1为本发明的较佳实施方式中的人造血管的制备流程示意图,请参见图i,该人造血管的制备方法包括如下步骤: [0027] Preparation of FIG. 1 a schematic flow chart of an exemplary embodiment of the invention the vascular prosthesis, see Figure i, the method for preparing an artificial blood vessel comprising the steps of:

[0028]步骤S1:裁剪织物并用手术缝合线将织物缝合成圆筒形,形成血管内层管壁;所述织物可以为涤纶、氨纶、蚕丝、聚四氟乙烯或它们的混纺材料;所述圆筒形织物的直径和所需人体血管直径相一致,优选直径范围为6mm〜38mm。 [0028] Step S1: cutting the fabric and the fabric with sutures sewn into a cylindrical shape, the inner wall of a vessel; the fabric may be polyester, spandex, silk, or blends thereof polytetrafluoroethylene material; the diameter cylindrical fabric and the desired diameter of the body vessel coincides, preferably a diameter in the range of 6mm~38mm.

[0029]步骤S2:将缝合后的圆筒形织物套设在与其直径相匹配的金属棒上,直接利用套有圆筒形织物的金属棒作为接收端,进行静电纺丝;使得纳米纤维沿圆筒形织物外表面圆周方向生长并均匀粘附在圆筒形织物的外表面,所述静电纺丝电压优选为10〜3〇kV,纺丝距离优选为5〜30mm,纺丝速度优选为〇. 1〜5ml/h。 [0029] Step S2: The fabric sewn cylindrical sleeve provided on its diameter to match the metal rod, the direct use of the fabric sleeve with a cylindrical metal rod as a receiver, electrostatic spinning; in such a nanofiber an outer circumferential surface of the cylindrical fabric growth direction and uniformly adhered to the outer surface of the cylindrical fabric, the electrostatic voltage is preferably 10~3〇kV spinning, the spinning distance is preferably 5~30mm, the spinning speed is preferably billion. 1~5ml / h.

[0030]步骤S3:当纳米纤维达到预设厚度后,停止纺丝,形成人造血管的外层管壁;从所述金属棒上取下人造血管。 [0030] Step S3: When the nanofiber reaches a predetermined thickness, the spinning is stopped, the outer wall forming an artificial blood vessel; removing the artificial vessel from the metal bar. 所述静电纺丝的原料为聚氨酯、聚乳酸、聚己内酯、聚乙烯醇、涤纶、聚甲基丙烯酸甲酯、聚苯乙烯、聚烯烃、硅橡胶以及它们之间的共聚物、胶原、壳聚糖、纤维素、明胶、纤维蛋白原或丝素蛋白;原料为溶液或熔融状态,溶液质量浓度优选为2.5〜 20%,在该浓度沮围下,纺丝稳走,电镜照片如图2b所不;溶液质量浓度如低于2.5%,纺丝为液滴状,电镜照片如图2a所示;溶液质量浓度如高于20%,则纺丝较粗且易堵针头,电镜照片如图2c所示。 The starting material is a polyurethane electrospinning, polylactic acid, polycaprolactone, polyvinyl alcohol, polyester, polymethyl methacrylate, polystyrene, polyolefins, silicone rubber, and among them copolymers, collagen, chitosan, cellulose, gelatin, fibrinogen, or silk fibroin; raw material solution or a molten state, the concentration of the solution is preferably 2.5~ 20%, at around this concentration ju, stable spinning down, electron micrograph of FIG. 2b are not; the solution concentration, such as below 2.5%, the spinning of droplets, the electron micrograph shown in Figure 2a; the solution concentration is higher than 20%, the spinning coarse and easy to block the needle, such as an electron micrograph FIG. 2c.

[0031]步骤S4:将纺丝完成的人造血管套在相应直径的弹性棒上,用特制的针刺模具对人造血管进行穿刺,转动人造血管或针刺模具,保证人造血管穿刺均匀,使得外面的纺丝层和内层织物结合更牢固。 [0031] Step S4: The finished spinning artificial blood vessel fitted over respective diameters of the elastic rod, to an artificial blood vessel puncture needle with a special mold, the mold is rotated or needle artificial blood vessels, artificial blood vessel puncture to ensure a uniform, so that the outside spinning and inner layers of fabric more firmly bound. 所述弹性棒的材料可以为橡胶、热塑性弹性体、泡沫塑料等有一定强度和弹性的材料。 The elastomeric material of the rod may have a certain strength and elasticity material such as rubber, thermoplastic elastomers, foams and the like. 在其它实施方式中,也可以使用水刺的方法完成对人造血管的穿刺,本发明对此不做限制。 In other embodiments, the spunlace method can be used to complete puncture of the vascular prosthesis, which is not limiting the present invention.

[0032]在本较佳实施方式中,针刺模具可以为两瓣或多瓣针刺模具。 [0032] In the present preferred embodiment, the mold may be two or acupuncture needle Orange Peel mold. 其中,图3和图4显示了两瓣针刺模具和四瓣针刺模具的横截面结构示意图。 Wherein FIG. 3 and FIG. 4 shows a schematic cross-sectional structure of the mold and four two needling needling mold. 如图3所示,针刺模具具有由两个弧形件1组成的环状主体,由弧形件1组成的环状主体具有一中心轴线(图中未示),弧形件1沿着此中心轴线纵向延伸。 3, the annular body having a needle die member 1 composed of two arc-shaped, annular body 1 by the arc-shaped member having a central axis component (not shown), along an arcuate member this central longitudinally extending axis. 弧形件1的半径与待穿刺的人造血管的半径相一致,在对人造血管进行针刺时,弧形件1与人造血管相贴合。 Radius to the radius of the artificial blood vessel to be punctured arcuate member 1 coincides, when the artificial blood vessel needling, the arcuate member 1 and bonded with an artificial blood vessel. 弧形件1还具有靠近所述中心轴线的内表面及远离所述中心轴线的外表面,针刺模具还具有多个刺针2,这些刺针2均匀布置于弧形件1的内表面上并指向所述中心轴线。 An arcuate member further having an inner surface and an outer surface adjacent said central axis away from the central axis, the mold also having a plurality of acupuncture needles 2, the needles 2 are arranged uniformly in the inner surface of the arcuate member 1 and points the central axis. 如图4所示,环状主体也可以由四个弧形件1组成,本发明对于弧形件的个数不做限制。 , The annular body 4 may be composed of four arc-shaped member 1, for the present invention is not limited in the number of arcuate member.

[0033]下面结合更具体的实施例对上述方案做进一步说明。 [0033] More specific embodiments in conjunction with the following Examples further illustrate the above embodiment. 实施例中采用的实施条件可以根据具体厂家的条件做进一步调整,未注明的实施条件通常为常规实验中的条件,在此不再——赘述。 Conditions employed in the embodiments can be further adjusted according to Embodiment particular manufacturer's conditions, conditions are generally not specified embodiment of a conventional test conditions, which is not - repeat.

[0034] 实施例1 [0034] Example 1

[0035] 将涤纶的机织物剪成100*30mm的尺寸,用手术缝合线缝合成长为l〇〇mm的管状,并套在合适大小的金属棒上。 [0035] The polyester fiber woven fabric cut into a size of 100 * 30mm, with surgical sutures tubular l〇〇mm grow and set in the metal bar of suitable size. 将金属棒作为静电纺丝设备的接收端,均匀旋转,转速为50r/ min。 The receiving end metal bars electrostatic spinning device, a uniform rotation speed is 50r / min. 将配置好的1〇%的聚乳酸溶液进行静电纺丝。 The configured 1〇% polylactic acid solution electrospinning. 纺丝电压为15kV;接收距离为15cm。 Spinning voltage was 15kV; reception distance is 15cm. 纺丝完成后,从金属棒上取下人造血管产品。 After the spinning is complete, remove the artificial blood vessels products from the metal bar. 将纺丝完成的人造血管套在相应直径的橡胶棒上, 用特制的针刺模具对人造血管进行针刺,转动人造血管,保证穿刺均匀。 The artificial blood vessel finished spinning sleeve on the respective rubber rod diameter of vascular prosthesis needled mold with a special needle, an artificial blood vessel is rotated, to ensure uniform puncture.

[0036] 实施例2 [0036] Example 2

[0037] 将蚕丝的机织物剪成100*20mm的尺寸,用手术缝合线缝合成长为100mm的管状,并套在合适大小的金属棒上。 [0037] The silk woven fabric cut into a size of 100 * 20mm, with surgical sutures grown to 100mm tubular and fit over the metal rods of suitable size. 将金属棒作为静电纺丝设备的接收端,均匀旋转,转速为50r/ min。 The receiving end metal bars electrostatic spinning device, a uniform rotation speed is 50r / min. 将配置好的20%的聚氨酯溶液进行静电纺丝。 The configured 20% polyurethane solution electrospinning. 纺丝电压为12kV;接收距离为20cm。 Spinning voltage of 12kV; reception distance is 20cm. 纺丝完成后,从金属棒上取下人造血管产品。 After the spinning is complete, remove the artificial blood vessels products from the metal bar. 将纺丝完成的人造血管套在相应直径的聚氨酯棒上,用特制的针刺模具对人造血管进行水刺,转动人造血管,保证穿刺均匀。 The finished spinning sleeve on the respective artificial blood vessel diameter polyurethane rod, artificial blood vessels for hydroentangling mold with a special needle, an artificial blood vessel is rotated, to ensure uniform puncture.

[0038] 实施例3 [0038] Example 3

[0039] 将氨纶的机织物剪成100*20mm的尺寸,用手术缝合线缝合成长为100mm的管状,并套在合适大小的金属棒上。 [0039] The spandex of the woven fabric cut into a size of 100 * 20mm, with surgical sutures grown to 100mm tubular and fit over the metal rods of suitable size. 将金属棒作为静电纺丝设备的接收端,均匀旋转,转速为50r/ min。 The receiving end metal bars electrostatic spinning device, a uniform rotation speed is 50r / min. 将配置好的8%的聚乙烯醇溶液进行静电纺丝。 The configured of 8% polyvinyl alcohol solution electrospinning. 纺丝电压为16kV;接收距离为15cm。 Spinning voltage of 16kV; reception distance is 15cm. 纺丝完成后,从金属棒上取下人造血管产品。 After the spinning is complete, remove the artificial blood vessels products from the metal bar. 将纺丝完成的人造血管套在相应直径的泡沫塑料棒上,用特制的针刺模具对人造血管进行针刺,转动人造血管,保证穿刺均匀。 The finished spinning sleeve on the respective artificial blood vessel diameter foam rods, artificial blood vessels for needled mold with a special needle, an artificial blood vessel is rotated, to ensure uniform puncture.

[0040]本发明提供的内喷式人造血管的制备方法,直接利用套有圆筒形织物的金属棒作为纳米纤维接收端进行静电纺丝,双层管壁的人造血管直接成型,具有如下优点:1、本发明采用的原料来源广泛,所用高分子材料均有商业化产品销售,也很容易合成得到,制备方法简单易行。 [0040] The process for preparing a jet vascular prosthesis of the present invention provides, directly a fabric sleeve with a cylindrical metal rod as a fiber electrospun nano-receiving end, double-wall vascular prostheses direct molding, has the following advantages : 1, a wide sources of raw materials used in the present invention, the polymer materials are commercially sold products, can be easily synthesized, preparation method is simple. 2、本发明制备的人造血管可以兼顾手术中防止管壁渗血和使细胞顺利长入的要求。 2, the preparation of vascular prosthesis of the present invention can prevent both the surgical bleeding and the cell wall ingrowth smooth requirements. 3、本发明采用的静电纺丝技术设备简单,容易操作,制备的人造血管是直接成型,避免损坏血管。 3, the present invention employs the electrospinning apparatus is simple, easy to operate, the preparation of vascular prosthesis is molded directly, to avoid damage to the blood vessels.

[0041]虽然本发明已以较佳实施例揭示如上,然其并非用以限定本发明,任何本领域技术人员,在不脱离本发明的精神和范围内,当可作些许的修改和完善,因此本发明的保护范围当以权利要求书所界定的为准。 [0041] While the present invention has been disclosed in the preferred embodiment described above, they are not intended to limit the present invention, anyone skilled in the art, without departing from the spirit and scope of the present invention, can make some modifications and improvement, Therefore, the scope of the invention as defined in the claims and their equivalents.

Claims (12)

  1. I. 一种人造血管的制备方法,其特征在于,包括如下步骤: 裁剪织物并将所述织物缝合成圆筒形,形成人造血管的内层管壁; 将所述内层管壁套设在金属棒上,将所述金属棒作为静电纺丝接收端,均匀旋转,进行静电纺丝,使得纳米纤维均匀粘附在所述内层管壁的外表面; 当所述纳米纤维达到预设厚度后,停止纺丝,形成所述人造血管的外层管壁;从所述金属棒上取下所述人造血管;以及将所述人造血管套在弹性棒上,对所述人造血管进行穿刺,使得所述外层管壁和所述内层管壁牢固结合。 I. A method of making a vascular prosthesis, characterized by comprising the steps of: cutting the fabric and the fabric sewn into a cylindrical shape, forming an artificial blood vessel inner wall; said inner sleeve provided in the wall metal rod, the metal rod receiving end as electrospinning, a uniform rotation, electrostatic spinning, such that the nanofibers uniformly adhered to an outer surface of said inner wall; when the thickness reaches the predetermined nanofiber after stopped spinning, forming an outer wall of the artificial blood vessel; removing the metal rod from the artificial blood vessel; artificial blood vessel and the elastic sleeve on the rod, the artificial blood vessel puncture, such that the outer wall and the inner wall strongly bonded.
  2. 2.如权利要求1所述的人造血管的制备方法,其特征在于,所述织物为机织或针织的织物结构。 2. The method of preparing an artificial blood vessel according to claim 1, wherein said fabric is a woven or knitted textile structure of.
  3. 3.如权利要求1所述的人造血管的制备方法,其特征在于,所述织物的材料选自涤纶、 氨纶、蚕丝、聚四氟乙烯中的一个或其中多个形成的混纺材料。 The method of preparing a vascular prosthesis according to claim 2, wherein said fabric material is selected from polyester, spandex, silk, polytetrafluoroethylene or a form in which a plurality of blended material.
  4. 4.如权利要求1所述的人造血管的制备方法,其特征在于,所述外层管壁的外径范围为6mm~38mm〇 4. A method of preparing an artificial blood vessel according to claim 1, wherein the outer wall diameter range of 6mm ~ 38mm〇
  5. 5.如权利要求1所述的人造血管的制备方法,其特征在于,所述静电纺丝的电压为5-50kV,纺丝距离为5-50mm,纺丝速度为0.01-10ml/h。 5. A method of preparing a vascular prosthesis according to claim 1, wherein the electrostatic spinning, the voltage was 5-50kV, spinning distance of 5-50mm, spinning rate 0.01-10ml / h.
  6. 6.如权利要求5所述的人造血管的制备方法,其特征在于,所述静电纺丝的电压为1〇〜 30kV,纺丝距离为5〜30ram,纺丝速度为0.1〜5ml/h。 Preparation 5 An artificial blood vessel as claimed in claim 6, wherein the electrostatic spinning, the voltage was 30kV 1〇~, spinning distance was 5~30ram spinning speed of 0.1~5ml / h.
  7. 7.如权利要求1所述的人造血管的制备方法,其特征在于,所述静电纺丝的原料选自聚氨酯、聚乳酸、聚己内酯、聚乙烯醇、涤纶、聚甲基丙烯酸甲酯、聚苯乙烯、聚烯烃、硅橡胶中的一个或其中多个的共聚物、胶原、壳聚糖、纤维素、明胶、纤维蛋白原或丝素蛋白。 7. A method of preparing a vascular prosthesis according to claim 1, wherein said electrostatic spinning material selected from polyurethane, polylactic acid, polycaprolactone, polyvinyl alcohol, polyester, polymethyl methacrylate , polystyrenes, polyolefins, silicone rubber wherein one or more copolymers of collagen, chitosan, cellulose, gelatin, fibrinogen, or silk fibroin.
  8. 8. 如权利要求7所述的人造血管的制备方法,其特征在于,所述静电纺丝的原料的溶液质量百分比浓度为2.5-20%。 The method of claim 7 prepared artificial blood vessel as claimed in claim 8, wherein the mass percent concentration of the electrostatic spinning solution is 2.5 to 20% starting material.
  9. 9. 如权利要求1所述的人造血管的制备方法,其特征在于,对所述人造血管进行穿刺的步骤包括对所述人造血管进行针刺或水刺。 9. A method of preparing an artificial blood vessel according to claim 1, wherein the step of puncturing the vascular prosthesis comprises an artificial blood vessel of the needling or hydroentangling.
  10. 10. 如权利要求1所述的人造血管的制备方法,其特征在于,所述弹性棒的材料为橡胶、 热塑性弹性体或泡沫塑料。 10. A method of preparing an artificial blood vessel according to claim 1, characterized in that the elastic material is a rubber rod, a thermoplastic elastomer or foam. II. 一种人造血管,其特征在于,通过如权利要求1-10中的任一方法制备得到。 II. An artificial blood vessels, characterized in that a preparation obtained by a method as claimed in any one of claims 1-10.
  11. 12. —种用于如权利要求1所述的人造血管穿刺的针刺模具,其特征在于,具有环状主体及多个刺针,所述环状主体具有中心轴线并且所述环状主体沿着所述中心轴线纵向延伸,所述环状主体具有靠近所述中心轴线的内表面及远离所述中心轴线的外表面,所述多个刺针均匀布置于所述环状主体的内表面上,并指向所述中心轴线。 12. - a kind of artificial blood vessel as claimed in claim 1, the puncture needle mold, characterized in that the annular body and having a plurality of needles, said annular body having a central axis and along said annular body said longitudinally extending central axis, said annular body having an inner surface and an outer surface adjacent said central axis away from the central axis, said plurality of needles are arranged uniformly on the inner surface of the annular body, and directed toward the central axis.
  12. 13. 如权利要求12所述的针刺模具,其特征在于,所述环状主体由多个弧形件组成,所述多个弧形件的半径与所述人造血管的半径相一致。 13. needling mold according to claim 12, characterized in that the annular body consisting of a plurality of arcuate members, said plurality of radius of the arcuate member with a radius of the artificial blood vessel coincide.
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