CN105301070B - A kind of PSA intelligent checking systems and its transducer production method - Google Patents
A kind of PSA intelligent checking systems and its transducer production method Download PDFInfo
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Abstract
The invention discloses a kind of intelligent checking system of trace detection prostate-specific antigen (PSA) and the preparation methods of its nucleus module electrochemical sensor.The present invention uses the modified electrodes such as the nanogold (platinum) of electrostatic spinning, magnetic nano-particle, and builds electrochemical sensor.Tracking examination object PSA is horizontal for a long time for PSA intelligent checking systems of the present invention, and doctor is helped more accurately to assess the risk that the examination object suffers from prostate cancer, to avoid the testing result of false positive, avoids over-treatment even wrong treatment.The present invention has the characteristics that sensitive, special, high selectivity, has important application prospect in medical clinic applications field.
Description
Technical field
The present invention relates to a kind of detecting system of prostate specific antigen more particularly to a kind of PSA intelligent checking systems and
Its transducer production method, belongs to field of biological detection.
Background technology
Prostate specific antigen (prostate specific antigen, PSA) is relevant a kind of anti-with prostate cancer
Original belongs to glycoprotein substance, is the preferable tumor markers of generally acknowledged diagnosis of prostate cancer.
The standard method of prior art assessment prostate cancer risk is to detect the prostate-specific antigen in blood
(PSA) level.
But there is clinical data to prove, PSA prostate cancer screenings method can not reduce the prostate cancer phase for the crowd of being screened
The death rate is closed, causes excessive unnecessary treatment instead.The reason for causing this problem is the examination side based on PSA levels
Though method, with higher sensitivity, its specificity is bad, therefore when examination object is extended to population from people at highest risk
When can cause more false positive, so as to generate over-treatment even wrong treatment.Therefore, a patient PSA is tracked for a long time
Level can help doctor more accurately to assess the risk that the patient suffers from prostate cancer.
Many of prior art detect PSA level method, but be not implemented it is highly selective while and build for a long time
Monitoring system.
Chinese patent CN101070345B discloses a kind of method for detecting prostate-specific antigen (PSA), make use of
The monoclonal antibody of the anti-prostate-specific-antigen of the patented invention is examined using PSA burdens detection matrix with Inhibition ELISA
PSA is surveyed, another method is the Double antibody sandwich ELISA detection PSA using the monoclonal antibody of anti-prostate-specific-antigen,
For the PSA detection ranges of monoclonal antibody up to 0~2048ng/mL, which is related to biological monoclonal antibody, right in detection method
The specific requirements of antibody are very high, and be otherwise present with a large amount of false positives as a result, other high to biological antibody preservation requirement, such as
It is improper to preserve, and great error can be caused in practical operation.
Chinese patent CN 101324576A disclose it is a kind of detect prostate-specific antigen (PSA) kit, be with
Prostate-specific antigen PSA, as standard items, overcomes existing with G types immunoglobulin domain (IgGFc) fusion protein
It is difficult to recombinantly express and purify the technological deficiency of the restructuring PSA close with natural PS A in technology, greatly reduces PSA detections
The manufacture cost of kit.But the accuracy to detection, anti-interference etc. do not make improvements.
Chinese patent CN104374908A discloses a kind of biosensor for detecting prostate-specific antigen (PSA),
A kind of immunosensor is prepared for using calcium in silver ion substituted hydroxy apatite, and applied to quick detection PSA, by PSA's
Detection is converted into the detection of silver ion, realizes higher sensitivity.But the invention does not refer to that system tracks detect.
The content of the invention
The defects of to overcome the prior art, the present invention prepare a kind of intelligence of trace detection prostate-specific antigen (PSA)
It can detecting system.
The intelligent checking system of the trace detection PSA includes：Electrochemical sensor module, intelligent storage module, wirelessly
Communication module, intelligent analysis module.
The electrochemical sensor module is made of the immune-electrochemistry sensor to PSA sensitivities.
The data transmission that the electrochemical sensor measurement obtains is to the intelligent storage module, then passes through the channel radio
News module transfers data to the intelligent analysis module, and PSA data trends are analyzed, and doctor is helped more accurately to assess
Patient suffers from the risk of prostate cancer.
The preparation method of the electrochemical sensor is as follows：
(1) electrostatic spinning：With nanogold suspension and the acetone soln of polylactic acid (PLA), it is organic outstanding to be configured to nanogold
Turbid；The organic suspension of the nanogold is added in electrospinning device liquid impellers, carries out electrostatic spinning, it is described to receive
The organic suspension of rice gold is ejected from 5~15 independent injector heads, and receiving terminal is the glass-carbon electrode of ground connection, injector head and glass carbon
The distance between electrode is 5-10cm, and spinning voltage 35-80kV, the electrostatic spinning time is 3~15min, after electrostatic spinning,
Glass-carbon electrode is not completely covered for obtained spinning film.
Wherein, the mass percent of the nanogold suspension is 0.5~2%, preferably 0.8~1.8%, further preferably
It is preferably 15~25wt% for 1%, the quality 10~30wt% of percent by volume of polylactic acid (PLA) acetone soln, it is further excellent
Elect 20wt% as, the mass ratio of nanogold suspension and PLA are 1:10~1:30, it is preferably 1:15~1:25, further preferably
For 1:20.
(2) preparation of magnetic nano-particle suspension：Add in chloride hydrate ferrous iron (FeCl2·4H2O) and 25% ammonium hydroxide
(NH3·H2O), stirred in constant temperature centrifugal blender with the speed of 5000~8000r/min, temperature is maintained at 90~95 DEG C, instead
It is centrifuged after answering, is cleaned with deionized water to pH neutrality, obtained solid is put into vacuum drying chamber 60~75
15~20h is dried in vacuo under the conditions of DEG C, grinds up to magnetic nano-particle, magnetic nano-particle is prepared into deionized water
Suspension.
Chloride hydrate ferrous iron (the FeCl2·4H2O) and the quality volume of ammonium hydroxide (g/mL) ratio is：1:150~1:200,
It is preferred that 1:160~1:190, further preferably 1:180.
(3) modified electrode is handled：The electrode that the electrostatic spinning that step (1) is obtained is processed is in the instrument with moving air
10~48h of middle placement removes the acetone solvent during electrostatic spinning；Before 8~15 0.001~10ng/mL of μ L concentration are added dropwise
Row gland specific antigen (PSA) solution is placed for 24 hours at room temperature to the electrode surface, be washed with deionized water it is net, then by step
(2) the nano-particle suspension is added drop-wise to electrode surface, places at room temperature for 24 hours, is washed with deionized water only to get modified electricity
Pole.
(4) prepared by electrochemical sensor：The modified electrode obtained with step (3) builds electrochemical sensor.
In one embodiment of the invention, nanogold can be substituted by Platinum Nanoparticles.
Technique effect：
(1) PSA intelligent checking systems of the present invention are horizontal by long-time tracking examination object PSA, help doctor more accurate
The risk that the examination object suffers from prostate cancer is assessed on ground, to avoid the testing result of false positive, avoids over-treatment even mistake
Treatment.
(2) metal nanoparticle adhered on the electrode by electrostatic spinning mode has preferable electric conductivity, can
Binding affinity analysis (PSA) substantially increases the selectivity of sensor.
(3) magnetic nano-particle separates and preenrichment analysis matter, can be immune so as to improve with amplification detection signal
The sensitivity of sensor.
(4) electrochemical immunosensor prepared by the present invention is used for the detection of prostate-specific antigen PSA, response time
Short, detection limit is low, and the range of linearity is wide, can realize simple, quick, highly sensitive and specific detection.
The present invention has the characteristics that sensitive, special, high selectivity, before having important application in medical clinic applications field
Scape.
Specific embodiment
Below by specific embodiment, further technical scheme is specifically described.It should be understood that below
Embodiment be intended only as illustrating, and do not limit the scope of the invention, while those skilled in the art is according to the present invention
The obvious change and modification made are also contained within the scope of the invention.
Embodiment 1
A kind of preparation method of electrochemical sensor, step are as follows：
(1) electrostatic spinning：With nanogold suspension and the acetone soln of polylactic acid (PLA), it is organic outstanding to be configured to nanogold
Turbid；The organic suspension of the nanogold is added in electrospinning device liquid impellers, carries out electrostatic spinning, it is described to receive
The organic suspension of rice gold is ejected from 5 independent injector heads, and receiving terminal is the glass-carbon electrode of ground connection, injector head and glass-carbon electrode
The distance between for 5cm, spinning voltage 35kV, the electrostatic spinning time is 3min, and after electrostatic spinning, obtained spinning film is not
Glass-carbon electrode is completely covered.
Wherein, the mass percent of the nanogold suspension is 0.5%, the mass body of polylactic acid (PLA) acetone soln
The mass ratio of product percentage 10wt%, nanogold suspension and PLA are 1:10.
(2) preparation of magnetic nano-particle suspension：Add in chloride hydrate ferrous iron (FeCl2·4H2O) and 25% ammonium hydroxide
(NH3·H2O), stirred in constant temperature centrifugal blender with the speed of 5000r/min, temperature is maintained at 95 DEG C, after reaction from
The heart separates, and is cleaned with deionized water to pH neutrality, obtained solid is put into vacuum drying chamber the vacuum under the conditions of 60 DEG C and is done
Dry 20h grinds up to magnetic nano-particle, magnetic nano-particle is prepared into suspension in deionized water.
Chloride hydrate ferrous iron (the FeCl2·4H2O) and the quality volume of ammonium hydroxide (g/mL) ratio is：1:150.
(3) modified electrode is handled：The electrode that the electrostatic spinning that step (1) is obtained is processed is in the instrument with moving air
Middle placement 10h removes the acetone solvent during electrostatic spinning；The prostate specific that 8 μ L concentration 0.001ng/mL are added dropwise resists
Former (PSA) solution is placed for 24 hours at room temperature to the electrode surface, be washed with deionized water it is net, then by step (2) nanometer
Particle suspension is added drop-wise to electrode surface, places at room temperature for 24 hours, is washed with deionized water only to get modified electrode.
(4) prepared by electrochemical sensor：The modified electrode obtained with step (3) builds electrochemical sensor.
A kind of intelligence of trace detection prostate-specific antigen (PSA) is built based on electrochemical sensor obtained
Detecting system.
The intelligent checking system of the trace detection PSA includes：Electrochemical sensor module, intelligent storage module, wirelessly
Communication module, intelligent analysis module.
The electrochemical sensor module is made of the immune-electrochemistry sensor to PSA sensitivities.
The data transmission that electrochemical sensor measurement obtains is to the intelligent storage module, then passes through the wireless telecommunications mould
Block transfers data to the intelligent analysis module, and PSA data trends are analyzed, and doctor is helped more accurately to assess patient
Suffer from the risk of prostate cancer.
Embodiment 2
A kind of intelligent checking system of trace detection prostate-specific antigen (PSA).
The intelligent checking system of the trace detection PSA includes：Electrochemical sensor module, intelligent storage module, wirelessly
Communication module, intelligent analysis module.
The electrochemical sensor module is made of the immune-electrochemistry sensor to PSA sensitivities.
The data transmission that electrochemical sensor measurement obtains is to the intelligent storage module, then passes through the wireless telecommunications mould
Block transfers data to the intelligent analysis module, and PSA data trends are analyzed, and doctor is helped more accurately to assess patient
Suffer from the risk of prostate cancer.
The preparation method of the electrochemical sensor, step are as follows：
(1) electrostatic spinning：With Platinum Nanoparticles suspension and the acetone soln of polylactic acid (PLA), it is organic outstanding to be configured to Platinum Nanoparticles
Turbid；The organic suspension of the Platinum Nanoparticles is added in electrospinning device liquid impellers, carries out electrostatic spinning, it is described to receive
The organic suspension of Miboplatin is ejected from 8 independent injector heads, and receiving terminal is the glass-carbon electrode of ground connection, injector head and glass-carbon electrode
The distance between for 6cm, spinning voltage 45kV, the electrostatic spinning time is 5min, and after electrostatic spinning, obtained spinning film is not
Glass-carbon electrode is completely covered.
Wherein, the mass percent of the Platinum Nanoparticles suspension is 0.8%, the mass body of polylactic acid (PLA) acetone soln
The mass ratio of product percentage 15wt%, Platinum Nanoparticles suspension and PLA are 1:15.
(2) preparation of magnetic nano-particle suspension：Add in chloride hydrate ferrous iron (FeCl2·4H2O) and 25% ammonium hydroxide
(NH3·H2O), stirred in constant temperature centrifugal blender with the speed of 6000r/min, temperature is maintained at 91 DEG C, after reaction from
The heart separates, and is cleaned with deionized water to pH neutrality, obtained solid is put into vacuum drying chamber the vacuum under the conditions of 62 DEG C and is done
Dry 19h grinds up to magnetic nano-particle, magnetic nano-particle is prepared into suspension in deionized water.
Chloride hydrate ferrous iron (the FeCl2·4H2O) and the quality volume of ammonium hydroxide (g/mL) ratio is：1:160.
(3) modified electrode is handled：The electrode that the electrostatic spinning that step (1) is obtained is processed is in the instrument with moving air
Middle placement 18h removes the acetone solvent during electrostatic spinning；The prostate specific that 10 μ L concentration 0.01ng/mL are added dropwise resists
Former (PSA) solution is placed for 24 hours at room temperature to the electrode surface, be washed with deionized water it is net, then by step (2) nanometer
Particle suspension is added drop-wise to electrode surface, places at room temperature for 24 hours, is washed with deionized water only to get modified electrode.
(4) prepared by electrochemical sensor：The modified electrode obtained with step (3) builds electrochemical sensor.
Embodiment 3
A kind of preparation method of electrochemical sensor, step are as follows：
(1) electrostatic spinning：With nanogold suspension and the acetone soln of polylactic acid (PLA), it is organic outstanding to be configured to nanogold
Turbid；The organic suspension of the nanogold is added in electrospinning device liquid impellers, carries out electrostatic spinning, it is described to receive
The organic suspension of rice gold is ejected from 10 independent injector heads, and receiving terminal is the glass-carbon electrode of ground connection, injector head and glass carbon electricity
The distance between pole is 7cm, and spinning voltage 60kV, the electrostatic spinning time is 8min, after electrostatic spinning, obtained spinning film
Glass-carbon electrode is not completely covered.
Wherein, the mass percent of the nanogold suspension is 1%, the quality volume of polylactic acid (PLA) acetone soln
Percentage is 20wt%, and the mass ratio of nanogold suspension and PLA are 1:20.
(2) preparation of magnetic nano-particle suspension：Add in chloride hydrate ferrous iron (FeCl2·4H2O) and 25% ammonium hydroxide
(NH3·H2O), stirred in constant temperature centrifugal blender with the speed of 7000r/min, temperature is maintained at 92 DEG C, after reaction from
The heart separates, and is cleaned with deionized water to pH neutrality, obtained solid is put into vacuum drying chamber the vacuum under the conditions of 65 DEG C and is done
Dry 18h grinds up to magnetic nano-particle, magnetic nano-particle is prepared into suspension in deionized water.
Chloride hydrate ferrous iron (the FeCl2·4H2O) and the quality volume of ammonium hydroxide (g/mL) ratio is：1:180.
(3) modified electrode is handled：The electrode that the electrostatic spinning that step (1) is obtained is processed is in the instrument with moving air
Middle placement for 24 hours, removes the acetone solvent during electrostatic spinning；The prostate-specific antigen of 12 μ L concentration 0.5ng/mL is added dropwise
(PSA) solution is placed for 24 hours at room temperature to the electrode surface, be washed with deionized water it is net, then by step (2) nanoparticle
Sub- suspension is added drop-wise to electrode surface, places at room temperature for 24 hours, is washed with deionized water only to get modified electrode.
(4) prepared by electrochemical sensor：The modified electrode obtained with step (3) builds electrochemical sensor.
A kind of intelligence of trace detection prostate-specific antigen (PSA) is built based on electrochemical sensor obtained
Detecting system.
The intelligent checking system of the trace detection PSA includes：Electrochemical sensor module, intelligent storage module, wirelessly
Communication module, intelligent analysis module.
The electrochemical sensor module is made of the immune-electrochemistry sensor to PSA sensitivities.
The data transmission that electrochemical sensor measurement obtains is to the intelligent storage module, then passes through the wireless telecommunications mould
Block transfers data to the intelligent analysis module, and PSA data trends are analyzed, and doctor is helped more accurately to assess patient
Suffer from the risk of prostate cancer.
Embodiment 4
A kind of preparation method of electrochemical sensor, step are as follows：
(1) electrostatic spinning：With nanogold suspension and the acetone soln of polylactic acid (PLA), it is organic outstanding to be configured to nanogold
Turbid；The organic suspension of the nanogold is added in electrospinning device liquid impellers, carries out electrostatic spinning, it is described to receive
The organic suspension of rice gold is ejected from 12 independent injector heads, and receiving terminal is the glass-carbon electrode of ground connection, injector head and glass carbon electricity
The distance between pole is 9cm, and spinning voltage 70kV, the electrostatic spinning time is 10min, after electrostatic spinning, obtained spinning
Glass-carbon electrode is not completely covered for film.
Wherein, the mass percent of the nanogold suspension is 1.8%, the mass body of polylactic acid (PLA) acetone soln
The mass ratio of product percentage 25wt%, nanogold suspension and PLA are 1:25.
(2) preparation of magnetic nano-particle suspension：Add in chloride hydrate ferrous iron (FeCl2·4H2O) and 25% ammonium hydroxide
(NH3·H2O), stirred in constant temperature centrifugal blender with the speed of 7500r/min, temperature is maintained at 94 DEG C, after reaction from
The heart separates, and is cleaned with deionized water to pH neutrality, obtained solid is put into vacuum drying chamber the vacuum under the conditions of 70 DEG C and is done
Dry 16h grinds up to magnetic nano-particle, magnetic nano-particle is prepared into suspension in deionized water.
Chloride hydrate ferrous iron (the FeCl2·4H2O) and the quality volume of ammonium hydroxide (g/mL) ratio is：1:190.
(3) modified electrode is handled：The electrode that the electrostatic spinning that step (1) is obtained is processed is in the instrument with moving air
Middle placement 36h removes the acetone solvent during electrostatic spinning；The prostate-specific antigen of 13 μ L concentration 1ng/mL is added dropwise
(PSA) solution is placed for 24 hours at room temperature to the electrode surface, be washed with deionized water it is net, then by step (2) nanoparticle
Sub- suspension is added drop-wise to electrode surface, places at room temperature for 24 hours, is washed with deionized water only to get modified electrode.
(4) prepared by electrochemical sensor：The modified electrode obtained with step (3) builds electrochemical sensor.
A kind of intelligence of trace detection prostate-specific antigen (PSA) is built based on electrochemical sensor obtained
Detecting system.
The intelligent checking system of the trace detection PSA includes：Electrochemical sensor module, intelligent storage module, wirelessly
Communication module, intelligent analysis module.
The electrochemical sensor module is made of the immune-electrochemistry sensor to PSA sensitivities.
The data transmission that electrochemical sensor measurement obtains is to the intelligent storage module, then passes through the wireless telecommunications mould
Block transfers data to the intelligent analysis module, and PSA data trends are analyzed, and doctor is helped more accurately to assess patient
Suffer from the risk of prostate cancer.
Embodiment 5
A kind of preparation method of electrochemical sensor, step are as follows：
(1) electrostatic spinning：With nanogold suspension and the acetone soln of polylactic acid (PLA), it is organic outstanding to be configured to nanogold
Turbid；The organic suspension of the nanogold is added in electrospinning device liquid impellers, carries out electrostatic spinning, it is described to receive
The organic suspension of rice gold is ejected from 15 independent injector heads, and receiving terminal is the glass-carbon electrode of ground connection, injector head and glass carbon electricity
The distance between pole is 10cm, and spinning voltage 80kV, the electrostatic spinning time is 15min, after electrostatic spinning, obtained spinning
Glass-carbon electrode is not completely covered for film.
Wherein, the mass percent of the nanogold suspension is 2%, the quality volume of polylactic acid (PLA) acetone soln
The mass ratio of percentage 30wt%, nanogold suspension and PLA are 1:30.
(2) preparation of magnetic nano-particle suspension：Add in chloride hydrate ferrous iron (FeCl2·4H2O) and 25% ammonium hydroxide
(NH3·H2O), stirred in constant temperature centrifugal blender with the speed of 8000r/min, temperature is maintained at 95 DEG C, after reaction from
The heart separates, and is cleaned with deionized water to pH neutrality, obtained solid is put into vacuum drying chamber the vacuum under the conditions of 75 DEG C and is done
Dry 15h grinds up to magnetic nano-particle, magnetic nano-particle is prepared into suspension in deionized water.
Chloride hydrate ferrous iron (the FeCl2·4H2O) and the quality volume of ammonium hydroxide (g/mL) ratio is：1:200.
(3) modified electrode is handled：The electrode that the electrostatic spinning that step (1) is obtained is processed is in the instrument with moving air
Middle placement 48h removes the acetone solvent during electrostatic spinning；The prostate-specific antigen of 15 μ L concentration 10ng/mL is added dropwise
(PSA) solution is placed for 24 hours at room temperature to the electrode surface, be washed with deionized water it is net, then by step (2) nanoparticle
Sub- suspension is added drop-wise to electrode surface, places at room temperature for 24 hours, is washed with deionized water only to get modified electrode.
(4) prepared by electrochemical sensor：The modified electrode obtained with step (3) builds electrochemical sensor.
A kind of intelligence of trace detection prostate-specific antigen (PSA) is built based on electrochemical sensor obtained
Detecting system.
The intelligent checking system of the trace detection PSA includes：Electrochemical sensor module, intelligent storage module, wirelessly
Communication module, intelligent analysis module.
The electrochemical sensor module is made of the immune-electrochemistry sensor to PSA sensitivities.
The data transmission that electrochemical sensor measurement obtains is to the intelligent storage module, then passes through the wireless telecommunications mould
Block transfers data to the intelligent analysis module, and PSA data trends are analyzed, and doctor is helped more accurately to assess patient
Suffer from the risk of prostate cancer.
Claims (6)
1. a kind of preparation method of PSA intelligent checking systems sensor, preparation process are as follows：
(1) electrostatic spinning：With nanogold suspension and the acetone soln of polylactic acid (PLA), the organic suspension of nanogold is configured to；
The organic suspension of the nanogold is added in electrospinning device liquid impellers, carries out electrostatic spinning, the nanogold
Organic suspension is ejected from 5~15 independent injector heads, and receiving terminal is the glass-carbon electrode of ground connection, injector head and glass-carbon electrode
The distance between for 5-10cm, spinning voltage 35-80kV, the electrostatic spinning time is 3~15min, after electrostatic spinning, is obtained
Spinning film glass-carbon electrode is not completely covered；
Wherein, the mass percent of the nanogold suspension is 0.5~2%, the quality volume of polylactic acid (PLA) acetone soln
The mass ratio of 10~30w/v% of percentage, nanogold suspension and polylactic acid (PLA) is 1:10~1:30；
(2) preparation of magnetic nano-particle suspension：Add in chloride hydrate ferrous iron (FeCl2·4H2O) and 25% ammonium hydroxide
(NH3·H2O), stirred in constant temperature centrifugal blender with the speed of 5000~8000r/min, temperature is maintained at 90~95 DEG C, instead
It is centrifuged after answering, is cleaned with deionized water to pH neutrality, obtained solid is put into vacuum drying chamber 60~75
15~20h is dried in vacuo under the conditions of DEG C, grinds up to magnetic nano-particle, magnetic nano-particle is prepared into deionized water
Suspension；
Chloride hydrate ferrous iron (the FeCl2·4H2O) and the mass volume ratio g/mL of ammonium hydroxide is：1:150~1:200；
(3) modified electrode is handled：The electrode that the electrostatic spinning that step (1) obtains is processed is put in the instrument with moving air
10~48h is put, removes the acetone solvent during electrostatic spinning；The prostate of 8~15 0.001~10ng/mL of μ L concentration is added dropwise
Specific antigen (PSA) solution is placed for 24 hours at room temperature to the electrode surface, be washed with deionized water it is net, then by step (2)
The nano-particle suspension is added drop-wise to electrode surface, places at room temperature for 24 hours, is washed with deionized water only to get modified electrode；
(4) prepared by electrochemical sensor：The modified electrode obtained with step (3) builds electrochemical sensor.
2. the preparation method of PSA intelligent checking systems sensor according to claim 1, it is characterised in that：Step (1)
The mass percent of the nanogold suspension is 0.8~1.8%, the quality percent by volume of polylactic acid (PLA) acetone soln
The mass ratio of 15~25w/v%, nanogold suspension and polylactic acid (PLA) is 1:15~1:25；
Step (2) chloride hydrate ferrous iron (FeCl2·4H2O) and the mass volume ratio g/mL of ammonium hydroxide is：1:160~1:190.
3. the preparation method of PSA intelligent checking systems sensor according to claim 2, it is characterised in that：Step (1) institute
The mass percent for stating nanogold suspension is 1%, and the quality percent by volume of polylactic acid (PLA) acetone soln is 20w/v%,
Nanogold suspension and the mass ratio of polylactic acid (PLA) are 1:20；
Step (2) chloride hydrate ferrous iron (FeCl2·4H2O) and the mass volume ratio g/mL of ammonium hydroxide is：1:180.
4. according to the preparation method of claims 1 to 3 any one of them PSA intelligent checking system sensors, it is characterised in that：
The nanogold is substituted by Platinum Nanoparticles.
5. a kind of intelligent checking system of trace detection PSA, it is characterised in that：The system is any based on Claims 1 to 4
What the preparation method of the PSA intelligent checking system sensors described in was prepared；
The intelligent checking system of the trace detection PSA includes：Electrochemical sensor module, intelligent storage module, wireless telecommunications
Module, intelligent analysis module；
The electrochemical sensor module is by the system of Claims 1 to 4 any one of them PSA intelligent checking system sensors
What Preparation Method was prepared；
The data transmission that the electrochemical sensor measurement obtains is to the intelligent storage module, then passes through the wireless telecommunications mould
Block transfers data to the intelligent analysis module, and prostate-specific antigen (PSA) data trend is analyzed, helps to cure
Raw more accurately assessment patient suffers from the risk of prostate cancer.
6. the intelligent checking system of trace detection PSA according to claim 5, it is characterised in that：The electrochemical sensing
Device module is made of the immune-electrochemistry sensor to prostate-specific antigen (PSA) sensitivity.
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| CN104374908A (en) * | 2014-11-07 | 2015-02-25 | 济南大学 | Preparation and application of immunosensor for calcium in silver ion replaced hydroxyapatite |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2776100T3 (en) * | 2006-03-31 | 2020-07-29 | Massachusetts Inst Technology | System for targeted delivery of therapeutic agents |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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