CN105296548A - Method for preparing succinic acid by using lotus seed peel wastes as fermentation raw material - Google Patents
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Abstract
A method for preparing succinic acid by using lotus seed peel wastes as a fermentation raw material comprises the following steps: 1, preprocessing the lotus seed peel wastes; 2, carrying out first stage culture: putting strains in a seed culture solution, and culturing for 14h; 3, carrying out second stage culture: inoculating 4% of a seed medium after the first stage culture, and culturing for 7h to obtain a culture solution; and 4, carrying out third stage culture: inoculating the culture solution to a fermentation medium, introducing 0.5-5L/min of carbon dioxide to a fermentation tank, feeding a sodium hydroxide solution with the mole concentration of 1mol/L in the fermentation process to maintain the pH value of the fermentation system in a range of 5.5-6.5, and fermenting at 37-39DEG C for 48h, and ending the fermentation when the sodium ion concentration reaches 2.2-2.5% in order to obtain succinic acid. The method has the advantages of low production cost, and realization of reduction of pollution to environment.
Description
Technical field
This patent relates to a kind of method using lotus skin powder to prepare succinic acid as fermentation raw material, belongs to technical field of bioengineering.
Background technology
Lotus skin powder be lotus seeds processing and produce by product.Along with lotus seeds product demand increases, the expansion of relevant enterprise scale and the raising of production efficiency, the process problem of waste material also highlights.
At present, effective Land use systems be there is no to lotus skin powder, to its research also rare report.Show according to pertinent data, can it be fermenting raw materials production ethanol, or adopt suitable extracting method extraction functional component wherein, thus effectively utilize waste resource.
Succinic acid (succinicacid) has another name called succsinic acid, is extensively present in organism, is a kind of important binary organic carboxyl acid, is also the important meta-bolites of microorganism tricarboxylic acid cycle glycolytic pathway.Succinic acid has a wide range of applications in a lot of field, can be used for synthesized degradable plastics, the tranquillizer of field of medicaments, the foodstuff additive of foodstuffs industry, tensio-active agent etc.Therefore, the production method that is efficient, low cost of succinic acid also becomes the focus of Recent study, and wherein adopting the method for biological fermentation to prepare succinic acid is the most important thing.
The Main way reducing biological fermentation process succinic acid preparation cost uses cheap carbon source, how effectively to utilize natural Biological resources, can meet the normal life requirement of people, can reduce again the pollution of environment is a significantly problem.Undoubtedly, fully utilize lotus skin powder to have important practical significance to enterprise and society.
Summary of the invention
The object of the invention is to effectively utilize waste resource, provide a kind of method using lotus skin powder to prepare succinic acid as fermentation raw material, use lotus skin powder to prepare succinic acid fermentation liquor as the carbon source of biological fermentation, reduce the cost that biological fermentation process prepares succinic acid.
The present invention is achieved by the following technical programs.
Use lotus skin powder to prepare a method for succinic acid as fermentation raw material, its preparation method is as follows:
(1) process of lotus skin powder
1. by lotus skin powder with 1% aqueous sodium hydroxide solution soak 2 hours under 120 degree of conditions, the mass ratio of lotus skin powder and aqueous sodium hydroxide solution is 1:3;
2. soak after terminating, leached by solid, use washed with de-ionized water, rinse to neutral, add citric acid or other organic acids adjustment pH value of solution to 4.0 ~ 4.5, temperature adjusts to 38 ~ 40 degree;
3. Novi is believed that cellulase Carezyme 4500L joins in the solution 2. processed through step (1) to be hydrolyzed, the time is 48 hours, and the dosage of Novi letter cellulase Carezyme 4500L is 10% of fibre content in lotus skin powder solids;
Solid filtering in the solution that 4. 3. will process through step (1), and collect filtrate, this filtrate is cellulase hydrolysis liquid, and in cellulase hydrolysis liquid, glucose content is 2.2%, and Xylose Content is about 0.4%; Solid is added deionized water by solid-liquid mass ratio 1:2, and use 1mol/L sodium hydroxide to adjust pH value to 5.5 ~ 5.8, add Novi's letter α-amylase, the dosage of Novi's letter α-amylase is 0.05% of starch content in solid, temperature is raised to 95 ~ 100 DEG C, carry out starch liquefacation and obtain liquefier, it is 60 ~ 80 minutes that liquefaction is held time;
5. liquefier is cooled to 60 ~ 65 DEG C, adjustment pH value is 4 ~ 4.5, and add Novi and believe that saccharifying enzyme carries out saccharification and obtains Glucoamylase hydrolysis liquid, in Glucoamylase hydrolysis liquid, glucose content is 8.5%, the dosage of Novi's letter saccharifying enzyme is 0.05% of starch content in solids, and saccharification time is 24 hours;
6. the hydrolysis sugar liquid that total sugar content is 350g/L is concentrated into after being mixed with Glucoamylase hydrolysis liquid by cellulase hydrolysis liquid;
(2) fermention medium is configured
Adopt deionized water preparation fermention medium 100mL, the moiety of fermention medium is counted by mass concentration: peptone 5g/L, corn steep liquor 10g/L, total reducing sugars content 100g/L, potassium primary phosphate 2.5g/L, Sodium phosphate dibasic 2.5g/L, yeast extract paste
5g/L, sodium acetate 1g/L, with deionized water preparation, wherein total reducing sugars takes from hydrolysis sugar liquid.
(3) one-level is cultivated
Actinobacillus succinogenes (Actinobacillussuccinogenes) CGMCC1593 is put into seed culture fluid and cultivates, and incubation time is 14h.
(4) secondary is cultivated
One-level connects seed culture medium after cultivating, and inoculum size is 4%, and incubation time obtains nutrient solution after reaching 7h.
(5) third stage culture
Nutrient solution is accessed fermention medium, in fermentor tank, the intake of carbonic acid gas is 0.5 ~ 5L/min, in fermenting process, stream adds volumetric molar concentration is that the pH value of the sodium hydroxide solution maintenance fermentation system of 1mol/L is between 5.5 ~ 6.5, temperature is 37 ~ 39 DEG C, fermentation time is 48h, during fermentation ends, Na ion concentration reaches 2.2% ~ 2.5%, obtains succinic acid after fermentation ends.
A kind of above-mentioned method using lotus skin powder to prepare succinic acid as fermentation raw material, wherein, preferably, in described fermenting process, stream adds volumetric molar concentration is that to maintain the pH value of fermentation system be 6.0 ~ 6.2 for the sodium hydroxide solution of 1mol/L.
A kind of above-mentioned method using lotus skin powder to prepare succinic acid as fermentation raw material, wherein, the cellulase of described use is Novi letter cellulase Carezyme 4500L.
A kind of above-mentioned method using lotus skin powder to prepare succinic acid as fermentation raw material, is characterized in that, the product after the Mierocrystalline cellulose that the carbon source that fermentation uses obtains through process for lotus skin powder, hydrolysis of hemicellulose.
Beneficial effect of the present invention is:
By the pre-treatment to lotus skin powder, removed by the xylogen in lotus skin powder, employing acidolysis and enzymolysis be the sugared source needed for fermentation succinic acid by hemicellulose and cellulose conversion, and this has great effect for the raw materials cost reducing Biological preparation succinic acid.Biological process is utilized to extract succinic acid, safety and environmental protection.The present invention adopts lotus skin powder to produce succinic acid, and industrial step reasonable in design, the succinic acid impurity of output is low, and utilization ratio is high.While low production cost, accomplish the requirement reduced the pollution of environment.
Embodiment
Lotus skin powder used in embodiment, first need detect lotus skin powder, main project comprises starch content, crude fiber content, protein content, ash oontent, water-content.
The detection of starch content, is mainly used the method for acidolysis to be hydrolyzed to starch, and is detected the glucose content in hydrolyzed solution by DNS method, then pushes away starch content by calculating is counter.
The mensuration of protein content, adopts micro-Kjeldahl, with reference to GB/T5009.5-1985.
Coarse-fibred mensuration, is by quantitative Semen Nelumbinis powder is carried out oxygenolysis in the mixed solution of the vitriol oil and potassium bichromate, and uses the anti-method of dripping excessive potassium permanganate of Sulfothiorine.
The mensuration of moisture, adopts direct drying method, with reference to GB/T-14769-93.
The mensuration of ash content, with reference to GB5009.4-85
According to the detection of above method, the lotus skin powder adopted in the embodiment of the present invention contains starch 33.5%, crude protein 11.9%, robust fibre 17.56%, moisture 12.69%, ash content 4.2%, and also have the component of 22.15% to fail to understand in addition, supposition may be xylogen.
As can be seen from the above results, material mainly starch and the Mierocrystalline cellulose as carbon source in lotus skin powder, can be utilized.
The TSB nutrient solution used in the embodiment of the present invention is pancreas peptone soybean broth.
Bacterial strain uses therefor Actinobacillus succinogenes (Actinobacillussuccinogenes) CGMCC1593 of the present invention, open in Chinese patent ZL200610038113.6, publication number CN1814747A, publication date on August 9th, 2006.
Below in conjunction with embodiment, the invention will be further described.
embodiment 1
Use lotus skin powder to prepare a method for succinic acid as fermentation raw material, its preparation method is as follows:
(1) process of lotus skin powder
1. take 100g lotus skin powder, use the aqueous sodium hydroxide solution of 1% to soak 2 hours under 120 degree of conditions, the mass ratio of lotus skin powder and aqueous sodium hydroxide solution is 1:3;
2. soak after terminating, leached by solid, use washed with de-ionized water, rinse to neutral, add citric acid adjustment pH value of solution to 4.0 ~ 4.5, temperature adjusts to 38 ~ 40 degree; 3. Novi is believed that cellulase Carezyme 4500L joins in the solution 2. processed through step (1) to be hydrolyzed, time is 48 hours, the dosage of Novi letter cellulase Carezyme 4500L is 10% of fibre content in lotus skin powder solids, and quality is 1.7g;
Solid filtering in the solution that 4. 3. will process through step (1), and collect filtrate, this filtrate is cellulase hydrolysis liquid; Solid is added deionized water by solid-liquid mass ratio 1:2, and uses 1mol/L sodium hydroxide to adjust pH value to 5.5 ~ 5.8, add Novi's letter α-amylase, the dosage of Novi's letter α-amylase is 0.05% of starch content in solid, and quality is 0.017g; Temperature is raised to 95 ~ 100 DEG C, carries out starch liquefacation and obtains liquefier, and it is 60 ~ 80 minutes that liquefaction is held time;
5. liquefier is cooled to 60 ~ 65 DEG C, the hydrochloric acid adjustment adjustment pH value using 1mol/L is 4 ~ 4.5, and add Novi and believe that saccharifying enzyme carries out saccharification and obtains Glucoamylase hydrolysis liquid, the dosage of Novi's letter saccharifying enzyme is 0.05% of starch content in solids, and quality is 0.017g; Saccharification time is 24 hours;
6. the hydrolysis sugar liquid that total sugar content is 350g/L is concentrated into after being mixed with Glucoamylase hydrolysis liquid by cellulase hydrolysis liquid;
(2) seed culture medium is configured
Preparation seed culture fluid 10mL, consist of peptone 5g/L, corn steep liquor 20g/L, glucose 20g/L, potassium primary phosphate 1.5g/L, Sodium phosphate dibasic 1.5g/L, yeast extract paste 5g/L, prepares with deionized water.
(3) fermention medium is configured
Adopt deionized water preparation fermention medium 100mL, the moiety of fermention medium is counted by mass concentration: peptone 5g/L, corn steep liquor 10g/L, total reducing sugars content 100g/L, potassium primary phosphate 2.5g/L, Sodium phosphate dibasic 2.5g/L, yeast extract paste
5g/L, sodium acetate 1g/L, with deionized water preparation, wherein total reducing sugars takes from hydrolysis sugar liquid.
(4) pre-treatment of fermention medium and seed culture medium
By seed culture medium and fermention medium all in Autoclave, temperature 121 DEG C, sterilizing 20min.
(5) one-level is cultivated
Actinobacillus succinogenes (Actinobacillussuccinogenes) CGMCC1593 is put into seed culture fluid and cultivates, and incubation time is 14h.
(6) secondary is cultivated
One-level connects seed culture medium after cultivating, and inoculum size is 4%, and incubation time obtains nutrient solution after reaching 7h.
(7) third stage culture
Nutrient solution is accessed fermention medium, in fermentor tank, the intake of carbonic acid gas is 3L/min, in fermenting process, stream adds volumetric molar concentration is that the pH value of the sodium hydroxide solution maintenance fermentation system of 1mol/L is between 6.0-6.1, temperature is 37 ~ 39 DEG C, fermentation time is 48h, during fermentation ends, Na ion concentration reaches 2.2% ~ 2.5%, obtains succinic acid after fermentation ends.
embodiment 2
Use lotus skin powder to prepare a method for succinic acid as fermentation raw material, its preparation method is as follows:
(1) process of lotus skin powder
1. take 100g lotus skin powder, use the aqueous sodium hydroxide solution of 1% to soak 2 hours under 120 degree of conditions, the mass ratio of lotus skin powder and aqueous sodium hydroxide solution is 1:3;
2. soak after terminating, leached by solid, use washed with de-ionized water, rinse to neutral, add succinic acid adjustment pH value of solution to 4.0, temperature adjusts to 38 degree; 3. Novi is believed that cellulase Carezyme 4500L joins in the solution 2. processed through step (1) to be hydrolyzed, time is 48 hours, the dosage of Novi letter cellulase Carezyme 4500L is 10% of fibre content in lotus skin powder solids, and quality is 1.7g;
Solid filtering in the solution that 4. 3. will process through step (1), and collect filtrate, this filtrate is cellulase hydrolysis liquid; Solid is added deionized water by solid-liquid mass ratio 1:2, and uses 1mol/L sodium hydroxide to adjust pH value to 5.5, add Novi's letter α-amylase, the dosage of Novi's letter α-amylase is 0.05% of starch content in solid, and quality is 0.017g; Temperature is raised to 95 DEG C, carries out starch liquefacation and obtains liquefier, and it is 60 minutes that liquefaction is held time;
5. liquefier is cooled to 60 DEG C, the hydrochloric acid adjustment adjustment pH value using 1mol/L is 4, and add Novi and believe that saccharifying enzyme carries out saccharification and obtains Glucoamylase hydrolysis liquid, the dosage of Novi's letter saccharifying enzyme is 0.05% of starch content in solids, and quality is 0.017g; Saccharification time is 24 hours;
6. the hydrolysis sugar liquid that total sugar content is 350g/L is concentrated into after being mixed with Glucoamylase hydrolysis liquid by cellulase hydrolysis liquid;
(2) seed culture medium is configured
Preparation seed culture fluid 10mL, consist of peptone 5g/L, corn steep liquor 20g/L, glucose 20g/L, potassium primary phosphate 1.5g/L, Sodium phosphate dibasic 1.5g/L, yeast extract paste 5g/L, prepares with deionized water.
(3) fermention medium is configured
Adopt deionized water preparation fermention medium 100mL, the moiety of fermention medium is counted by mass concentration: peptone 5g/L, corn steep liquor 10g/L, total reducing sugars content 100g/L, potassium primary phosphate 2.5g/L, Sodium phosphate dibasic 2.5g/L, yeast extract paste
5g/L, sodium acetate 1g/L, with deionized water preparation, wherein total reducing sugars takes from hydrolysis sugar liquid.
(4) pre-treatment of fermention medium and seed culture medium
By seed culture medium and fermention medium all in Autoclave, temperature 121 DEG C, sterilizing 20min.
(5) one-level is cultivated
Actinobacillus succinogenes (Actinobacillussuccinogenes) CGMCC1593 is put into seed culture fluid and cultivates, and incubation time is 14h.
(6) secondary is cultivated
One-level connects seed culture medium after cultivating, and inoculum size is 4%, and incubation time obtains nutrient solution after reaching 7h.
(7) third stage culture
Nutrient solution is accessed fermention medium, in fermentor tank, the intake of carbonic acid gas is 3L/min, in fermenting process, stream adds volumetric molar concentration is that the pH value of the sodium hydroxide solution maintenance fermentation system of 1mol/L is between 6.0-6.1, temperature is 37 ~ 39 DEG C, fermentation time is 48h, during fermentation ends, Na ion concentration reaches 2.2% ~ 2.5%, obtains succinic acid after fermentation ends.
embodiment 3
Use lotus skin powder to prepare a method for succinic acid as fermentation raw material, its preparation method is as follows:
(1) process of lotus skin powder
1. take 100g lotus skin powder, use the aqueous sodium hydroxide solution of 1% to soak 2 hours under 120 degree of conditions, the mass ratio of lotus skin powder and aqueous sodium hydroxide solution is 1:3;
2. soak after terminating, leached by solid, use washed with de-ionized water, rinse to neutral, add acetic acid adjustment pH value of solution to 4.5, temperature adjusts to 40 degree; 3. Novi is believed that cellulase Carezyme 4500L joins in the solution 2. processed through step (1) to be hydrolyzed, time is 48 hours, the dosage of Novi letter cellulase Carezyme 4500L is 10% of fibre content in lotus skin powder solids, and quality is 1.7g;
Solid filtering in the solution that 4. 3. will process through step (1), and collect filtrate, this filtrate is cellulase hydrolysis liquid; Solid is added deionized water by solid-liquid mass ratio 1:2, and uses 1mol/L sodium hydroxide to adjust pH value to 5.8, add Novi's letter α-amylase, the dosage of Novi's letter α-amylase is 0.05% of starch content in solid, and quality is 0.017g; Temperature is raised to 100 DEG C, carries out starch liquefacation and obtains liquefier, and it is 80 minutes that liquefaction is held time;
5. liquefier is cooled to 65 DEG C, the hydrochloric acid adjustment adjustment pH value using 1mol/L is 4.5, and add Novi and believe that saccharifying enzyme carries out saccharification and obtains Glucoamylase hydrolysis liquid, the dosage of Novi's letter saccharifying enzyme is 0.05% of starch content in solids, and quality is 0.017g; Saccharification time is 24 hours;
6. the hydrolysis sugar liquid that total sugar content is 350g/L is concentrated into after being mixed with Glucoamylase hydrolysis liquid by cellulase hydrolysis liquid;
(2) seed culture medium is configured
Preparation seed culture fluid 10mL, consist of peptone 5g/L, corn steep liquor 20g/L, glucose 20g/L, potassium primary phosphate 1.5g/L, Sodium phosphate dibasic 1.5g/L, yeast extract paste 5g/L, prepares with deionized water.
(3) fermention medium is configured
Adopt deionized water preparation fermention medium 100mL, the moiety of fermention medium is counted by mass concentration: peptone 5g/L, corn steep liquor 10g/L, total reducing sugars content 100g/L, potassium primary phosphate 2.5g/L, Sodium phosphate dibasic 2.5g/L, yeast extract paste
5g/L, sodium acetate 1g/L, with deionized water preparation, wherein total reducing sugars takes from hydrolysis sugar liquid.
(4) pre-treatment of fermention medium and seed culture medium
By seed culture medium and fermention medium all in Autoclave, temperature 121 DEG C, sterilizing 20min.
(5) one-level is cultivated
Actinobacillus succinogenes (Actinobacillussuccinogenes) CGMCC1593 is put into seed culture fluid and cultivates, and incubation time is 14h.
(6) secondary is cultivated
One-level connects seed culture medium after cultivating, and inoculum size is 4%, and incubation time obtains nutrient solution after reaching 7h.
(7) third stage culture
Nutrient solution is accessed fermention medium, in fermentor tank, the intake of carbonic acid gas is 3L/min, in fermenting process, stream adds volumetric molar concentration is that the pH value of the sodium hydroxide solution maintenance fermentation system of 1mol/L is between 6.0-6.1, temperature is 37 ~ 39 DEG C, fermentation time is 48h, during fermentation ends, Na ion concentration reaches 2.2% ~ 2.5%, obtains succinic acid after fermentation ends.
The succinic acid yield result that how obtained table 1 embodiment of the present invention 1-3 is:
| Succinic acid output | Unit | Numerical value |
| Embodiment 1 | g/L | 45 |
| Embodiment 2 | g/L | 40 |
| Embodiment 3 | g/L | 38 |
Beneficial effect of the present invention is:
By the pre-treatment to lotus skin powder, removed by the xylogen in lotus skin powder, employing acidolysis and enzymolysis be the sugared source needed for fermentation succinic acid by hemicellulose and cellulose conversion, and this has great effect for the raw materials cost reducing Biological preparation succinic acid.Biological process is utilized to extract succinic acid, safety and environmental protection.The present invention adopts lotus skin powder to produce succinic acid, and industrial step reasonable in design, the succinic acid impurity of output is low, and utilization ratio is high.While low production cost, accomplish the requirement reduced the pollution of environment.
Above embodiment is only used to further illustrate the present invention; can not be interpreted as limiting the scope of the invention, some nonessential improvement that those skilled in the art's foregoing according to the present invention is made and adjustment all belong to protection scope of the present invention.
Claims (4)
1. use lotus skin powder to prepare a method for succinic acid as fermentation raw material, it is characterized in that, its preparation method is as follows:
(1) process of lotus skin powder
1. by lotus skin powder with 1% aqueous sodium hydroxide solution soak 2 hours under 120 degree of conditions, the mass ratio of lotus skin powder and aqueous sodium hydroxide solution is 1:3;
2. soak after terminating, solid leached, use washed with de-ionized water, rinse to neutral, add citric acid or other organic acids (adjustment pH value of solution to 4.0 ~ 4.5, temperature adjusts to 38 ~ 40 degree;
3. Novi is believed that cellulase Carezyme 4500L joins in the solution 2. processed through step (1) to be hydrolyzed, the time is 48 hours, and the dosage of Novi letter cellulase Carezyme 4500L is 10% of fibre content in lotus skin powder solids;
Solid filtering in the solution that 4. 3. will process through step (1), and collect filtrate, this filtrate is cellulase hydrolysis liquid, and in cellulase hydrolysis liquid, glucose content is 2.2%, and Xylose Content is about 0.4%; Solid is added deionized water by solid-liquid mass ratio 1:2, and use 1mol/L sodium hydroxide to adjust pH value to 5.5 ~ 5.8, add Novi's letter α-amylase, the dosage of Novi's letter α-amylase is 0.05% of starch content in solid, temperature is raised to 95 ~ 100 DEG C, carry out starch liquefacation and obtain liquefier, it is 60 ~ 80 minutes that liquefaction is held time;
5. liquefier is cooled to 60 ~ 65 DEG C, adjustment pH value is 4 ~ 4.5, and add Novi and believe that saccharifying enzyme carries out saccharification and obtains Glucoamylase hydrolysis liquid, in Glucoamylase hydrolysis liquid, glucose content is 8.5%, the dosage of Novi's letter saccharifying enzyme is 0.05% of starch content in solids, and saccharification time is 24 hours;
6. the hydrolysis sugar liquid that total sugar content is 350g/L is concentrated into after being mixed with Glucoamylase hydrolysis liquid by cellulase hydrolysis liquid;
(2) fermention medium is configured
Adopt deionized water preparation fermention medium 100mL, the moiety of fermention medium is counted by mass concentration: peptone 5g/L, corn steep liquor 10g/L, total reducing sugars content 100g/L, potassium primary phosphate 2.5g/L, Sodium phosphate dibasic 2.5g/L, yeast extract paste
5g/L, sodium acetate 1g/L, with deionized water preparation, wherein total reducing sugars takes from hydrolysis sugar liquid;
(3) one-level is cultivated
Actinobacillus succinogenes (Actinobacillussuccinogenes) CGMCC1593 is put into seed culture fluid and cultivates, and incubation time is 14h;
(4) secondary is cultivated
One-level connects seed culture medium after cultivating, and inoculum size is 4%, and incubation time obtains nutrient solution after reaching 7h;
(5) third stage culture
Nutrient solution is accessed fermention medium, in fermentor tank, the intake of carbonic acid gas is 0.5 ~ 5L/min, in fermenting process, stream adds volumetric molar concentration is that the pH value of the sodium hydroxide solution maintenance fermentation system of 1mol/L is between 5.5 ~ 6.5, temperature is 37 ~ 39 DEG C, fermentation time is 48h, during fermentation ends, Na ion concentration reaches 2.2% ~ 2.5%, obtains succinic acid after fermentation ends.
2. use lotus skin powder to prepare a method for succinic acid as fermentation raw material, it is characterized in that, preferably, in described fermenting process, stream adds volumetric molar concentration is that to maintain the pH value of fermentation system be 6.0 ~ 6.2 for the sodium hydroxide solution of 1mol/L.
3. use lotus skin powder to prepare a method for succinic acid as fermentation raw material, it is characterized in that, the cellulase of described use is Novi letter cellulase Carezyme 4500L.
4. use lotus skin powder to prepare a method for succinic acid as fermentation raw material, it is characterized in that, the product after the Mierocrystalline cellulose that the carbon source that fermentation uses obtains through process for lotus skin powder, hydrolysis of hemicellulose.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101215583A (en) * | 2008-01-18 | 2008-07-09 | 南京工业大学 | Method for preparing succinic acid by coupling fermentation and membrane separation unit |
| CN101215582A (en) * | 2007-12-28 | 2008-07-09 | 江南大学 | Method for producing succinic acid by fermenting straw raw material |
| CN101948883A (en) * | 2010-09-06 | 2011-01-19 | 长春工业大学 | Method for fermentatively producing microbial oil by utilizing corn husk dregs as raw materials |
| CN102242158A (en) * | 2011-05-26 | 2011-11-16 | 湘潭大学 | Method for producing ethanol with lotus peel powder as raw material by synchronization of saccharification and fermentation |
-
2015
- 2015-11-25 CN CN201510826761.7A patent/CN105296548A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101215582A (en) * | 2007-12-28 | 2008-07-09 | 江南大学 | Method for producing succinic acid by fermenting straw raw material |
| CN101215583A (en) * | 2008-01-18 | 2008-07-09 | 南京工业大学 | Method for preparing succinic acid by coupling fermentation and membrane separation unit |
| CN101948883A (en) * | 2010-09-06 | 2011-01-19 | 长春工业大学 | Method for fermentatively producing microbial oil by utilizing corn husk dregs as raw materials |
| CN102242158A (en) * | 2011-05-26 | 2011-11-16 | 湘潭大学 | Method for producing ethanol with lotus peel powder as raw material by synchronization of saccharification and fermentation |
Non-Patent Citations (1)
| Title |
|---|
| 李嫦: "莲皮粉的综合利用研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
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