CN105288747A - Curcumin-carried polylactic acid biological scaffold and preparation method thereof - Google Patents
Curcumin-carried polylactic acid biological scaffold and preparation method thereof Download PDFInfo
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- CN105288747A CN105288747A CN201510852174.5A CN201510852174A CN105288747A CN 105288747 A CN105288747 A CN 105288747A CN 201510852174 A CN201510852174 A CN 201510852174A CN 105288747 A CN105288747 A CN 105288747A
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- curcumin
- polylactic acid
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Abstract
The invention belongs to the field of medical treatment, and particularly relates to a curcumin-carried polylactic acid biological scaffold and a preparation method thereof. A dichloromethane solution of polylactic acid and a dichloromethane solution of curcumin are mixed, the mixed solution is injected into a beaker fixed on a platform and filled with ethyl alcohol through an injection pump on a rapid prototype at a set flow speed, and the needle head of the injection pump is moved at a certain speed according to the designed shape of the biological scaffold and computer editing program, so that the stable curcumin-carried polylactic acid biological scaffold is formed in an ethanol coagulating bath. According to the invention, the curcumin-carried polylactic acid biological scaffold prepared through the preparation method provided by the invention can be used for being implanted into a lesion area for direct administration such as bone cancer and a new approach of low-bioavailability medicines on clinical administration is provided.
Description
Technical field
The invention belongs to medical field, particularly relate to polylactic acid biological support carrying curcumin and preparation method thereof.
Background technology
Curcumin is a kind of plant polyphenol extracted from zingiberaceous plant Rhizoma Curcumae Longae (CurcumaLongaL.) rhizome, is the principle active component in Turmeric.Modern pharmacology research discloses the extremely strong physiologically active of curcumin, and verified its of research has significant antioxidation and antitumor action.At present, the clinical trial in a large number about curcumin anti-tumor activity is extensively carried out, and mainly concentrates on for cancer of pancreas and colon cancer.In addition, the clinical research of various safety evaluatio indicates the safety of oral curcumin, and in day clothes metering up under the heavy dose of 8g to 12g, curcumin does not still cause any obvious toxic and side effects.Tentative confirmation in the absorption of curcumin and bioavailability large quantity research in early days, an early stage zoopery shows, after giving the curcumin of rat 1g/kg, the curcumin of about 75% is directly discharged from intestinal, and only only have the prototype of trace to detect in urine, prompting curcumin prototype may can not enter in the middle of blood circulation again.After adopting intravenous injection to give rat curcumin, find that curcumin meeting active transport is in bile, and most of prototype generation metabolic response, if the liver organization suspension extracted is mixed with curcumin, there is metabolism in the curcumin of 90%, demonstrate in liver organization the metabolic enzyme that there is stronger curcumin chemical compounds in 30 minutes.This research indicates that curcumin difficulty absorbs, the physiological disposition feature of tachymetabolism and rapid drainage first.
Summary of the invention
The object of the present invention is to provide polylactic acid biological support of a kind of year curcumin and preparation method thereof, be intended to low bioavailability, the problem such as tachymetabolism and rapid drainage of avoiding curcumin oral administration, new route of administration is opened up in the clinical practice for curcumin.
The present invention is achieved in that the polylactic acid biological support carrying curcumin, by following preparation method gained:
Step one, is dissolved in polylactic acid in dichloromethane, stirs three hours to obtain uniform PLA solution, is dissolved in by curcumin in dichloromethane, and gentle agitation two hours is to obtain uniform curcumin solution;
Step 2, adds to the PLA solution prepared in curcumin solution, stirs three hours to obtain uniform solution;
Step 3, the solution of preparation is placed on the glass syringe being furnished with stainless pin, use geometric parameter and the processing method of Matlab software design support, rapid prototyping machine is injected into be fixed on platform by syringe pump is filled in the beaker of ethanol with the flow velocity of setting, the shape appearance that syringe pump syringe needle designs according to biological support, move with certain speed according to Computerized Editing program, in alcohol solidification bath, form the stable polylactic acid biological support carrying curcumin.
Preferably, described PLA solution w/v concentration is 10%, and curcumin concentration is 1.5mg/mL, and coagulating bath is ethanol and aqueous mixtures, and the v/v ratio of second alcohol and water is 70/30 or 65/35.
Ejection of syringe pump parameter is preferably: solution flow rate (F) is 0.8 ~ 1.0mL/h, lamination volume (V
dep) be 600mm/min, interlayer pin displacement (d
z) be 0.08mm.
The shape of carrying the polylactic acid biological support of curcumin obtained by the method is circular and square.Products therefrom measures by UV method the curcumin being actually loaded to support and the Rhizoma Curcumae Longae being dissolved in coagulating bath in the fabrication process usually calculates drug loading, under 425nm, carry out ultra-violet analysis.
Polylactic acid, also referred to as polylactide, is a kind of novel Biodegradable material, is be that primary raw material is polymerized the polymer obtained with lactic acid, belongs to polyester family.The mechanical strength good due to it and toughness, polylactic acid is widely used at biomedical sector, can produce disposable infusion apparatus, exempt to tear type operation suture thread etc. open.Polylactic acid is widely used as the timbering material of organizational project, acid and the oligomer of monomer is become mainly through hydrolytic degradation at human body, by to breathe and kidney filters and discharges, eliminating metal rack class can not degrade, and is difficult to taking-up and stays in the body for a long time bring the shortcomings such as damage to tissue.
Polylactic acid biological support of provided by the invention year curcumin and preparation method thereof, what obtain carries the polylactic acid biological support of curcumin and can improve the low bioavailability of curcumin and tachymetabolism and excretion, to playing a role in oncotherapy.
Detailed description of the invention
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Specific embodiments of the invention:
Embodiment 1
Be dissolved in by polylactic acid in dichloromethane, stirring three hours is 10% solution to obtain uniform w/v, and be dissolved in by curcumin in dichloromethane, within two hours, to obtain uniform solution, concentration is 1.5mg/mL to gentle agitation, the polylactic acid prepared is added in curcumin solution, stir three hours to obtain uniform solution, the solution of preparation is placed on the glass syringe being furnished with stainless pin, with the geometric parameter of Matlab software design support, comprise the distance 0.5mm between cellosilk, layer thickness 4.16mm, geometry is circular, set up processing method, the flow velocity of syringe pump is 0.8mL/h, lamination speed is that 600mm/min is injected in the beaker being fixed on and platform filling ethanol and aqueous mixtures by syringe pump with the flow velocity of setting in rapid prototyping machine, second alcohol and water v/v is 65:35, the shape appearance that syringe pump syringe needle designs according to biological support, move with certain speed according to Computerized Editing program, the stable polylactic acid biological support carrying curcumin is formed in alcohol solidification bath, vacuum drying stores.
Embodiment 2
Be dissolved in by polylactic acid in dichloromethane, stir three hours to obtain uniform w/v10% solution, be dissolved in by curcumin in dichloromethane, within two hours, to obtain uniform solution, concentration is 1.5mg/mL to gentle agitation, the polylactic acid prepared is added in curcumin solution, stir three hours to obtain uniform solution, the solution of preparation is placed on the glass syringe being furnished with stainless pin, with the geometric parameter of Matlab software design support, comprise the distance 0.5mm between cellosilk, layer thickness 4.16mm, geometry is square, set up processing method, the flow velocity of syringe pump is 0.8mL/h, lamination speed be 600mm/min in rapid prototyping machine by syringe pump with the flow velocity of setting be injected into be fixed on platform fills v/v be 65/35 ethanol and aqueous mixtures beaker in, the shape appearance that syringe pump syringe needle designs according to biological support, move with certain speed according to Computerized Editing program, the stable polylactic acid biological support carrying curcumin is formed in alcohol solidification bath, vacuum drying stores.
Embodiment 3
Be dissolved in by polylactic acid in dichloromethane, stirring three hours is 10% solution to obtain uniform w/v, and be dissolved in by curcumin in dichloromethane, within two hours, to obtain uniform solution, concentration is 1.5mg/mL to gentle agitation, the polylactic acid prepared is added in curcumin solution, stir three hours to obtain uniform solution, the solution of preparation is placed on the glass syringe being furnished with stainless pin, with the geometric parameter of Matlab software design support, comprise the distance 0.5mm between cellosilk, layer thickness 4.16mm, geometry is circular, set up processing method, the flow velocity of syringe pump is 1.0mL/h, lamination speed be 600mm/min in rapid prototyping machine by syringe pump with the flow velocity of setting be injected into be fixed on platform fills v/v be 70/30 ethanol and aqueous mixtures beaker in, the shape appearance that syringe pump syringe needle designs according to biological support, move with certain speed according to Computerized Editing program, the stable polylactic acid biological support carrying curcumin is formed in alcohol solidification bath, vacuum drying stores.
Embodiment 4
Be dissolved in by polylactic acid in dichloromethane, stirring three hours is 10% solution to obtain uniform w/v, and be dissolved in by curcumin in dichloromethane, within two hours, to obtain uniform solution, concentration is 1.5mg/mL to gentle agitation, the polylactic acid prepared is added in curcumin solution, stir three hours to obtain uniform solution, the solution of preparation is placed on the glass syringe being furnished with stainless pin, with the geometric parameter of Matlab software design support, comprise the distance 0.5mm between cellosilk, layer thickness 4.16mm, geometry is square, set up processing method, the flow velocity of syringe pump is 1.0mL/h, lamination speed be 600mm/min in rapid prototyping machine by syringe pump with the flow velocity of setting be injected into be fixed on platform fills v/v be 70/30 ethanol and aqueous mixtures beaker in, the shape appearance that syringe pump syringe needle designs according to biological support, move with certain speed according to Computerized Editing program, the stable polylactic acid biological support carrying curcumin is formed in alcohol solidification bath, vacuum drying stores.
Embodiment products therefrom, the curcumin being actually loaded to support is measured and the Rhizoma Curcumae Longae being dissolved in coagulating bath in the fabrication process usually calculates drug loading by UV method, under 425nm, carry out ultra-violet analysis, the drug loading recording the polylactic acid biological support of each embodiment download curcumin is as shown in table 1.
The carrying drug ratio (average ± standard deviation, n=3) of different support under each embodiment of table 1
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (3)
1. the polylactic acid biological support of year curcumin, is characterized in that, prepare gained by following methods:
Step one, is dissolved in polylactic acid in dichloromethane, stirs and obtain PLA solution, is dissolved in by curcumin in dichloromethane, stirs and obtains curcumin solution;
Step 2, adds to PLA solution in curcumin solution, stirs and obtain mixed liquor;
Step 3, mixed liquor is placed on the glass syringe being furnished with stainless pin, according to geometric parameter and the processing method of design support, rapid prototyping machine is injected into be fixed on platform by syringe pump is filled in the beaker of ethanol with the flow velocity of setting, the shape appearance that syringe pump syringe needle designs according to biological support, move with the speed of setting according to Computerized Editing program, finally in alcohol solidification bath, form the stable polylactic acid biological support carrying curcumin with shape.
2. the polylactic acid biological support carrying curcumin as claimed in claim 1, it is characterized in that, the PLA solution concentration w/v described in step one is 10%, and curcumin concentration is 1.5mg/mL; Coagulating bath described in step 3 is the mixture of second alcohol and water, and the v/v ratio of second alcohol and water is 70:30 or 65:35.
3. the polylactic acid biological support carrying curcumin as claimed in claim 1, it is characterized in that, the flow velocity of the setting described in step 3 is 0.8 ~ 1.0mL/h, and lamination volume is 600mm/min, and the displacement of interlayer pin is 0.08mm.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020183830A1 (en) * | 2001-06-01 | 2002-12-05 | Shih-Horng Su | Expandable biodegradable polymeric stents for combined mechanical support and pharmacological or radiation therapy |
| CN101279111A (en) * | 2008-05-15 | 2008-10-08 | 哈尔滨工程大学 | Method for preparing blood vessel stent with polyester medicament eluting coating |
| CN104241117A (en) * | 2013-06-09 | 2014-12-24 | 中芯国际集成电路制造(上海)有限公司 | Imaging method |
| CN104274867A (en) * | 2014-03-10 | 2015-01-14 | 北京阿迈特医疗器械有限公司 | Degradable polymer support as well as forming method and application thereof |
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2015
- 2015-11-27 CN CN201510852174.5A patent/CN105288747B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020183830A1 (en) * | 2001-06-01 | 2002-12-05 | Shih-Horng Su | Expandable biodegradable polymeric stents for combined mechanical support and pharmacological or radiation therapy |
| CN101279111A (en) * | 2008-05-15 | 2008-10-08 | 哈尔滨工程大学 | Method for preparing blood vessel stent with polyester medicament eluting coating |
| CN104241117A (en) * | 2013-06-09 | 2014-12-24 | 中芯国际集成电路制造(上海)有限公司 | Imaging method |
| CN104274867A (en) * | 2014-03-10 | 2015-01-14 | 北京阿迈特医疗器械有限公司 | Degradable polymer support as well as forming method and application thereof |
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