CN105271514B - Biological denitrification method and its application based on anaerobic ferrite oxidization ammonia - Google Patents

Biological denitrification method and its application based on anaerobic ferrite oxidization ammonia Download PDF

Info

Publication number
CN105271514B
CN105271514B CN201510775812.8A CN201510775812A CN105271514B CN 105271514 B CN105271514 B CN 105271514B CN 201510775812 A CN201510775812 A CN 201510775812A CN 105271514 B CN105271514 B CN 105271514B
Authority
CN
China
Prior art keywords
reactor
sewage
feammox
ammonia
anaerobic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201510775812.8A
Other languages
Chinese (zh)
Other versions
CN105271514A (en
Inventor
张代钧
姚宗豹
李玉莲
万新宇
何强
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chongqing University
Original Assignee
Chongqing University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chongqing University filed Critical Chongqing University
Priority to CN201510775812.8A priority Critical patent/CN105271514B/en
Publication of CN105271514A publication Critical patent/CN105271514A/en
Application granted granted Critical
Publication of CN105271514B publication Critical patent/CN105271514B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

The invention discloses a kind of biological denitrification method based on anaerobic ferrite oxidization ammonia, comprise the following steps:The sludge containing Feammox microorganisms is added in anaerobic reactor, ammonia sewage will be contained and be passed through in reactor, the NH in sewage is aoxidized using Fe (III) NTA4 +Generate NO2 , then denitrogenation processing is carried out, concretely comprise the following steps:First, culture enrichment Feammox microorganisms:1) sludge pretreatment, the 2) culture of Feammox microorganisms;2nd, the NH in Feammox microorganism treating sewages is utilized4 +:1) sewage disinfection treatment, 2) utilize the progress sewage disposal of Feammox microorganisms;3rd, the liquid being collected into after foregoing anaerobism Feammox processing is subjected to denitrogenation processing.This method has lower energy consumption, less carbon source demand, less greenhouse gas emission, less NO3 The advantages such as generation, investment and operating cost are few, have a extensive future.

Description

Biological denitrification method and its application based on anaerobic ferrite oxidization ammonia
Technical field
The invention belongs to technical field of sewage, and in particular to ammonia in a kind of ammonia treatment sewage using anaerobic ferrite oxidization Method and its application in sewage water denitrification.
Background technology
NH can be realized in sewage disposal at present4 +The microbial process of oxidation is mainly aerobic ammonia oxidation and anaerobism ammonia Oxidation.Although traditional aerobic ammonia oxidation process extensive use in sewage disposal, the technique needs logical from outside NH can be tested by entering oxygen4 +Oxidation, while oxygen and product NO in aerobic ammonia oxidation system be present2 -, therefore in system NOB be usually there will be by NO2 -It is oxidized to NO3 -, then can be by NO in process denitrification process3 -It is reduced to N2Realize NH4 +Removal, therefore traditional nitration denitrification technique needs additional O2, organic matter, while sludge yield is high.Although Anaerobic ammonium oxidation process can be under anaerobic by NH4 +Oxidation, but need to provide NO2 -To realize NH4 +Oxidation, therefore The technique, which needs to be combined together with aerobic ammonia oxidation process, could realize NH4 +Oxidation.
Iron is one kind of transition metal, and it is the high metallic element of earth's crust content second.Fe and C, O, N, S redox Reaction drives the biochemical cycles in the whole world.Microorganism alienation Fe (III) reduction is an important biology and geochemistry mistake Journey, not only the distribution to iron and mineralogy form have an impact, and because microorganism alienation Fe (III) reduction process couples The oxidative degradation of organic matter, be gradually applied to biological metallurgy, heavy metal (uranium, chromium, arsenic etc.) pollution environment it is biological prosthetic And the biodegradation of petrochemical industry class organic sewage.Fe also functions to one in the chemistry and microbiological oxidation reduction process of the material containing N Fixed effect, including greenhouse gases N2O generation.The microorganism of Fe (III) iron reduction can be carried out in nature from archeobacteria to thin Strain class is various.However, the biological chemical performance of microorganism Fe (III) reduction at present is still newer research field.
It has now been found that generally existing can be realized in anaerobism in the environment such as rice field, deposit, Freshwater ecosystems Under the conditions of rely on Fe (III) oxidations NH4+ phenomenon, the phenomenon is referred to as Feammox (iron oxidation ammonia), finds Feammox phenomenons Document such as:1) in river deposit:(the Ammonium oxidation coupled to dissimilatory such as Clement Reduction ofiron under anaerobic conditions in wet land soils, 2005);2) wetland is given birth to State system:The such as Shrestha (Oxidation ofAmmonium to Nitrite Under Iron-Reducing Conditions in Wetland Soils Laboratory,Field Demonstrations,and Push-Pull Rate Determination, 2009);3) mountain soil:(the Nitrogen loss from soil through such as Yang anaerobic ammonium oxidation coupled to iron reduction,2012);4) riverbank meadow:Huang (the Characterization ofincubation experiments and development ofan such as and Jaffe enrichment culture capable ofammonium oxidation under iron-reducing Conditions, 2015);5) anaerobically digested sludge:(the Possibility ofanoxic ferric such as Sawayama Ammonium oxidation.Journal ofBioscience and Bioengineering, 2006);6) paddy soil: The such as Ding (Nitrogen Loss throughAnaerobic Ammonium Oxidation Coupled to Iron Reduction from Paddy Soils in a Chronosequence,2014);7) freshwater lake deposit:Melton Deng (Microbial iron (II) oxidation in littoral freshwater lake sediment:the potential for competition between phototrophic vs.nitrate-reducing iron(II)- oxidizers,2012).Existing research thinks that Feammox reaction principles are as follows
Jaff é of Princeton university etc. are found that Feammox bacteriums in the soil on riverbank meadow, can realize Feammox, they take pedotheque from scene, are passed through in the lab using anaerobic reactor by additional ferrihydrite or goethite 180 days enrichment cultures Feammox bacteriums are crossed, but because the dissolubility of iron in ferrihydrite or goethite is very low, biology can profit Poor with property, concentration effect is not very good.
Feammox microorganisms can be under anaerobic by NH4 +It is oxidized to NO2 -, by the process and sewage disposal system Anammox (Anammox) technique combine, it becomes possible to realize under anaerobic by NH4 +It is converted into N2Process, Relative to traditional nitrification-denitrification technique, the coupling technique is by with lower energy consumption, less carbon source demand, less Greenhouse gas emission (such as N2O), less NO3 -The advantage such as generation and lower sludge yield.Therefore this reaction is at sewage It is with a wide range of applications in terms of reason.
Although iron content in ground surface environment, deposit is very high, Fe (III) solubility only has 10 under neutrallty condition- 9Mol, in most cases Fe (III) is present with insoluble, insoluble Fe oxides in aquifer.This is just limited A large amount of presence of Fe-ammox microorganisms have been made, therefore by lifting the content of bioavailability Fe (III), can be effective Promote Fe-ammox processes, such as by chelating agents such as NTA (nitrilotriacetic acid), EDTA (ethylenediamine tetra-acetic acid) and polyphosphate In the presence of form soluble chelating iron, and antimicrobial surface is transported to by diffusion, so as to lift Feammox mistakes Journey.
The content of the invention
It is an object of the invention to provide a kind of energy consumption is low, investment and operating cost are few, have a extensive future based on detesting Oxygen iron aoxidizes the biological denitrification method of ammonia.
Technical scheme is as follows:A kind of biological denitrification method based on anaerobic ferrite oxidization ammonia, comprises the following steps: The sludge containing Feammox microorganisms is added in anaerobic reactor, ammonia sewage will be contained and be passed through in reactor, utilized Fe (III) NH in NTA oxidation sewage4 +Generate NO2 -, then denitrogenation processing is carried out, comprise the following steps that:
First, culture enrichment Feammox microorganisms
1) sludge pretreatment:Introduces a collection sludge containing Feammox microorganisms is mixed to get mud mixture with nutrient solution, The nutrient solution is mixed by nutrient solution and trace element solution and obtain, its composition and ratio be addition 1.0 in every liter of nutrient solution~ 1.5mL trace element solution;Contain 0.16~0.20mM Fe (III) NTA, 2.00~2.30mMNH in the nutrient solution4 +And ore deposit Material;
2) culture of Feammox microorganisms:Foregoing mud mixture is accessed in anaerobic reactor, first exposes N2Mixed to muddy water Dissolved oxygen is 0mg/L in compound, and then bring into operation reactor, adds the foregoing nutrient solution of the mud mixture half volume 180~240min is run, supernatant, discharge rate and the culture added are then discharged after 15~25min of precipitation out of service, precipitation Liquid product is identical, so far completes a process cycle;Again the culture with discharged fluid volume same volume is added in reactor Liquid, start a new process cycle, multiple cycles of operation are completed in so circulation;The running temperature of reactor is 30~35 DEG C, Mud mixture pH in reactor is controlled 7.0~8.0;Reacted mud mixture is discharged from reactor once a day / 15th of cumulative volume abandons, and then fills into the new nutrient solution with abandoning liquid product same volume again;Altogether operation 70~ 110 days;
2nd, the NH in Feammox microorganism treating sewages is utilized4 +
1) sewage disinfection treatment:0.16~0.20mM Fe (III) NTA, mineral matter dissolving are added in pending sewage containing ammonia Mixed liquor is uniformly mixed so as to obtain, then the ratio addition trace element solution of 1.0~1.5mL trace element solutions is added in every liter of mixed liquor It is mixed to get sewage disinfection treatment liquid;
2) add after the liquid for half volume of the mud mixture obtained after foregoing enrichment culture Feammox microorganisms being drained Enter and sewage disposal is carried out in anaerobic reactor:The foregoing dirt with mud mixture same volume in reactor is added in the reactor Discharge and the sewage added are pre- after 15~25min of water pretreatment liquid operation 180~240min and then precipitation out of service, precipitation The solution for the treatment of fluid same volume simultaneously collects the liquid, so far completes a process cycle;Again in reactor add with The sewage disinfection treatment liquid of displaced volume same volume, start a new process cycle, so circulation can be continued for dirt Water process;The running temperature of reactor is 30~35 DEG C, and the mud mixture pH in reactor is controlled 7.0~8.0;Daily one It is secondary 1st/15th of reacted mud mixture cumulative volume is discharged out of reactor to abandon, then fill into again and discharge liquor The sewage disinfection treatment liquid of same volume;So far complete the NH in sewage4 +It is converted into NO2 -Process;
3rd, the liquid being collected into after foregoing anaerobism Feammox processing is subjected to denitrogenation processing.
Preferably, the concentration of Fe (III) NTA in the nutrient solution is 0.18mM, NH4 +Concentration be 2.15mM, The concentration of Fe (III) NTA in the sewage disinfection treatment liquid is 0.18mM.
Preferably, described Fe (III) NTA is by Fe2(SO4)31 is pressed with NTA:1 mol ratio is matched somebody with somebody, described NH4 + From ammonium sulfate or ammonium chloride.
In the above-mentioned technical solutions, the mineral component and proportioning included in the nutrient solution and the sewage disinfection treatment liquid It is:NaH2PO40.04~0.06g/L, CaCl2·2H20.2~0.4g/L of O, MgSO4·7H2O0.2~0.4g/L, KHCO3 1.0~1.5g/L, FeSO40.0060~0.0065g/L, 0.0060~0.0065g/L of EDTA;Described trace element solution Include the component of following concentration:13~17g/L of EDTA, H3BO40.010~0.016g/L, MnCl2·4H2O 0.90~ 1.10g/L、CuSO4·5H20.20~0.30g/L of O, ZnSO4·7H20.35~0.50g/L of O, NiCl2·6H2O 0.15~ .025g/L、Na2SeO4·10H20.15~0.26g/L of O, Na2MoO4·2H20.17~0.27g/L of O, Na2WO4·2H2O 0.03~0.07g/L.
Preferably, the mineral component and proportioning included in the nutrient solution and the sewage disinfection treatment liquid be: NaH2PO4 0.05g/L、CaCl2·2H2O 0.3g/L、MgSO4·7H2O 0.3g/L、KHCO3 1.25g/L、FeSO4 0.00625g/L、EDTA 0.00625g/L;The trace element solution includes the component of following concentration:EDTA 15g/L、H3BO4 0.014g/L、MnCl2·4H2O 0.99g/L、CuSO4·5H2O 0.25g/L、ZnSO4·7H2O 0.43g/L、NiCl2·6H2O 0.19g/L、Na2SeO4·10H2O 0.21g/L、Na2MoO4·2H2O 0.22g/L、Na2WO4·2H2O 0.05g/L。
Preferably, the method that the liquid being collected into after the processing by anaerobism Feammox carries out denitrogenation processing is to detest Anaerobic ammonium oxidation PROCESS FOR TREATMENT, by the NO in solution2 -It is converted into N2
In the above-mentioned technical solutions, the anaerobic reactor is sbr reactor device either UASB reactors or EGSB reaction Device.
Preferably, the trace element solution addition is:It is molten that 1.25mL trace elements are added in every liter of nutrient solution Liquid, every liter of mixed liquor adds 1.25mL trace element solutions during sewage disinfection treatment.
Preferably, the running temperature in reactor is 30~32 DEG C, and the mud mixture pH controls in reactor exist 7.0~7.5.
The principle of this method is that Feammox microorganisms are utilized under anaerobic condition by the NH in sewage4 +It is oxidized to NO2 -, with reference to Existing NO2 -Denitrification treatment process, you can realize the NH in sewage4 +It is converted into N2So as to realize that sewage water denitrification is handled.
Another object of the present invention is to provide the above-mentioned biological denitrification method based on anaerobic ferrite oxidization ammonia in sewage water denitrification Application in processing.
The beneficial effects of the invention are as follows:Compared to traditional two kinds of techniques of aerobic ammonia oxidation or Anammox, the present invention Method can be under anaerobic by NH4 +It is oxidized to NO2 -, subsequently only need to combine existing ripe Anammox (Anammox) technique can realizes NH4 +Removal, the two technical process are all anaerobic processes, and condition is easily controlled, and is removed from The O needed in aerobic treatment process2, save cost;Relative to traditional nitrification-denitrification technique, coupling technique tool There are lower energy consumption, less carbon source demand, less greenhouse gas emission (such as N2O), less NO3 -The advantages such as generation.Should Method energy consumption is low, investment and operating cost are few, has good application prospect.
Brief description of the drawings
Fig. 1 is that Fe (III) NTA aoxidizes NH in sbr reactor device in the embodiment of the present invention4 +Process chart;Wherein, 1SBR reactors, 2 hollow sandwiches, 3 water-bath imports, 4 water-baths outlet, 5 water-bath pumps, 6 water-baths, 7 intake pumps, 8 inlet water tanks, 9 go out Water pump, 10 water tanks, 11pH meters, 12 online dissolved oxygen analytic instruments, 13 magnetic stirring apparatus, 14 ventholes.
Embodiment
Describe the embodiment of the present invention in detail below in conjunction with technical scheme and accompanying drawing, but the present invention not only limits to In example is implemented as follows.
The Fe (III) of the inventive method is carried out using the sbr reactor device that the total reaction volume in Fig. 1 is 3L in laboratory NTA aoxidizes NH4 +, introduces a collection sludge is derived from University Of Chongqing's Zi Huan institutes water treatment experiment room iron oxidation ammonia (Feammox) sludge introduces a collection (the second pond sludge for deriving from Chongqing sewage treatment plant).Concrete operation step is as follows:
First, culture enrichment Feammox microorganisms
(1) sludge pretreatment:1000ml foregoing introduces a collection sludge is taken, dirt is rinsed with nutrient solution in the case where continuously exposing condition of nitrogen gas After mud 3-5 times, the mud mixture after 2L is handled is settled to nutrient solution.The nutrient solution is by nutrient solution and trace element Solution is mixed and obtained, and its composition and ratio is that 1.25mL trace element solutions are added in every liter of nutrient solution.
Each component content is in every liter of nutrient solution:Fe2(SO4)30.036 gram, (NH4)2SO40.14 gram or NH4Cl 0.113 gram, 0.034 gram of NTA, NaH2PO4 0.05g、CaCl2·2H2O 0.3g、MgSO4·7H2O0.3g、KHCO3 1.25g、 FeSO4 0.00625g、EDTA 0.00625g。
Each component content exists in every liter of trace element solution:EDTA 15g、H3BO4 0.014g、MnCl2·4H2O0.99g、 CuSO4·5H2O 0.25g、ZnSO4·7H2O 0.43g、NiCl2·6H2O 0.19g、Na2SeO4·10H2O 0.21g、 Na2MoO4·2H2O 0.22g、Na2WO4·2H2O 0.050g。
(2) culture of Feammox microorganisms:It will be carried out in foregoing obtained 2L mud mixture access sbr reactor device 1 Spawn incubation, the nutrient solution prepared are stored in inlet water tank 8, first expose N2It is 0mg/L to dissolved oxygen, then bring into operation SBR Reactor 1, a cycle of operation are 240min, run under stirring 210min (in the 12min of beginning by intake pump 7 from 1L nutrient solutions are pumped out in inlet water tank 8 into sbr reactor device 1), stop stirring precipitation 18min and then water outlet after operation 210min 12min (pumps out the reacted mud mixtures of 1L to water tank 10) by going out water pump 9 out of sbr reactor device 1, so far completes one Process cycle.Then 1L nutrient solutions are pumped out again out of inlet water tank 8 by intake pump 7 to enter in sbr reactor device 1, start one newly Process cycle, so circulation completes multiple cycles of operation.The running temperature of reactor is 30~32 DEG C, the muddy water in reactor 7.0~7.5, magnetic stirrer speed is 100~200rpm for mixture pH controls.Once a day out of sbr reactor device 1 Discharge the reacted mud mixtures of 200ml to abandon, then add the new nutrient solutions of 200ml.(many experiments prove within 90 days for operation altogether Operation can realize the object of the invention in 70~110 days), it is enriched with the Feammox microorganisms in sludge, obtained sludge is i.e. Available for NH in sewage4 +Oxidation.Also connecing for Feammox microorganisms in sludge can be carried out with other reactors or miscellaneous equipment Kind and culture.The store method of sludge after enrichment Feammox microorganisms is:The sludge being enriched with is taken, with described nutrient solution Continuously exposing N2In the case of wash three times, will wash after sewage sludge storage in light tight anaerobism vial, add 0.5L detest Oxygen nutrient solution simultaneously seals, and is placed on and is preserved in 4 DEG C of refrigerator and can use and (can generally preserve 6 months) as introduces a collection sludge.
2nd, the NH in Feammox microorganism treating sewages is utilized4 +
This step is still in the NH carried out in the sbr reactor device 1 shown in Fig. 1 in Feammox microorganism treating sewages4 +.Such as Fruit is the culture enrichment of the Feammox microorganisms carried out in miscellaneous equipment, by what is obtained after enrichment culture Feammox microorganisms Sludge is accessed in the anaerobic reactor that carry out sewage disposal.
(1) sewage disinfection treatment:Containing 2.15mM NH4 +Treatment sewage in add the following component matched as follows and obtain To sewage disinfection treatment liquid:Fe is added in every liter of sewage2(SO4)30.036 gram, 0.034 gram of NTA, NaH2PO4 0.05g、 CaCl2·2H2O 0.3g、MgSO4·7H2O 0.3g、KHCO3 1.25g、FeSO40.00625g, EDTA 0.00625g dissolve Mix, then add foregoing 1.25mL trace element solutions mixing in the solution;Sewage disinfection treatment liquid is stored in sbr reactor In inlet water tank 8 in device 1 (if be previously used for cultivate Feammox microorganisms same sbr reactor device, original is entered Nutrient solution discharge in water tank 8 is changed to sewage disinfection treatment liquid), expose N in inlet water tank 82Dissolved oxygen into sewage is 0mg/L, reach anaerobic condition.
(2) N is being exposed from the sbr reactor device 1 after foregoing enrichment culture Feammox microorganisms2In the case of discharge 1L liquid Body, then proceed by sewage disposal:Each cycle of operation of sbr reactor device 1 is 240min during sewage disposal, under stirring Operation 210min (pumps out 1L sewage disinfection treatments liquid out of inlet water tank 8 by intake pump 7 in the 12min of beginning and enters sbr reactor device In 1), stop stirring precipitation 18min, water outlet 12min after operation 210min and (pump out 1L out of sbr reactor device 1 by going out water pump 9 Sewage after reason is to water tank 10);So far a process cycle is completed.Then 1L is pumped out again out of inlet water tank 8 by intake pump 7 Sewage disinfection treatment liquid enters in sbr reactor device 1, starts a new process cycle, and so circulation can be continued for sewage Processing.The running temperature of reactor is 30~32 DEG C, and mud mixture pH in reactor control is 7.0~7.5, magnetic agitation Device mixing speed is 100~200rpm.The discharge reacted mud mixtures of 200ml are lost out of sbr reactor device 1 once a day Abandon, then add the foregoing sewage disinfection treatment liquid of 200ml.
NH is determined in sewage disposal process4 +Oxidation effectiveness, continuous operation 4h is tested, in pending sewage NH4 +For 2.15mM, in continuous 4h test, NH4 +Average oxidation speed be 156.16mgN/ (Ld), in water tank 10 The NO in water after the processing being collected into2 -Average accumulated speed be also 156.16mgN/ (Ld).
3rd, the sewage after anaerobism Feammox processing carries out Anammox processing
The water outlet being collected into water tank 10 after foregoing anaerobic ferrite oxidization ammonia treatment is subjected to Anammox processing, anaerobism Ammonia oxidation process is existing very ripe technique, be will not be described here.By anaerobic ammonia oxidation process by the NO in water2 -It is converted into N2, realize NH in sewage4 +Anaerobism remove.
By experiment, each component is matched somebody with somebody in the nutrient solution, sewage disinfection treatment liquid, trace element solution described in the inventive method Than can also realize the inventive method in following scope:The mineral matter group included in described nutrient solution and sewage disinfection treatment liquid Point:NaH2PO40.04~0.06g/L, CaCl2·2H20.2~0.4g/L of O, MgSO4·7H20.2~0.4g/L of O, KHCO3 1.0~1.5g/L, FeSO40.0060~0.0065g/L, 0.0060~0.0065g/L of EDTA;Fe in described nutrient solution2 (SO4)30.032~0.04 gram, (NH4)2SO40.13~0.15 gram or NH40.11~0.12 gram of Cl, NTA 0.03~ 0.034 gram;Fe in described sewage disinfection treatment liquid2(SO4)30.032~0.04 gram, 0.03~0.034 gram of NTA;Described is micro- Secondary element solution includes the component of following concentration:13~17g/L of EDTA, H3BO40.010~0.016g/L, MnCl2· 4H2O0.90~1.10g/L, CuSO4·5H20.20~0.30g/L of O, ZnSO4·7H20.35~0.50g/L of O, NiCl2· 6H20.15~.025g/L of O, Na2SeO4·10H20.15~0.26g/L of O, Na2MoO4·2H2O0.17~0.27g/L, Na2WO4·2H20.03~0.07g/L of O;
The inventive method is realized the NH in sewage4 +NO is converted under anaerobism anaerobic condition2 -, by the process and dirt Anaerobic ammonia oxidation process in water treatment system combines, and realizes under anaerobic by NH4 +It is eventually converted into N2Process, Relative to traditional nitrification-denitrification technique, the coupling technique is by with lower energy consumption, less carbon source demand, less Greenhouse gas emission (such as N2O), less NO3 -The advantages such as generation.

Claims (9)

1. a kind of biological denitrification method based on anaerobic ferrite oxidization ammonia, it is characterised in that comprise the following steps:In anaerobic reactor Middle sludge of the addition containing Feammox microorganisms, will contain ammonia sewage and be passed through in sbr reactor device, be aoxidized using Fe (III) NTA dirty NH in water4 +Generate NO2 , then denitrogenation processing is carried out, comprise the following steps that:
First, culture enrichment Feammox microorganisms
1) sludge pretreatment:Introduces a collection sludge containing Feammox microorganisms is mixed to get mud mixture with nutrient solution, it is described Nutrient solution is mixed by nutrient solution and trace element solution and obtained, and its composition and ratio is that 1.0~1.5mL of addition is micro- in every liter of nutrient solution Secondary element solution;Contain 0.16~0.20mM Fe (III) NTA, 2.00~2.30mM NH in the nutrient solution4 +And mineral matter;
2) culture of Feammox microorganisms:Foregoing mud mixture is accessed in anaerobic reactor, first exposes N2To mud mixture Middle dissolved oxygen is 0mg/L, and then bring into operation reactor, adds the foregoing nutrient solution operation of the mud mixture half volume 210min, supernatant then is discharged after precipitation 18min out of service, precipitation, discharge rate is identical with the nutrient solution volume added, extremely This completes a process cycle;Again the nutrient solution with discharged fluid volume same volume is added in reactor, starts one newly Process cycle, so circulation completes multiple cycles of operation;The running temperature of reactor is 30~35 DEG C, the muddy water in reactor Mixture pH is controlled 7.0~8.0;Discharge reacted mud mixture cumulative volume from reactor once a day 15 points One of abandon, then fill into again with abandon liquid product same volume new nutrient solution;Run 70~110 days altogether;
2nd, the NH in Feammox microorganism treating sewages is utilized4 +
1) sewage disinfection treatment:0.16~0.20mM Fe (III) NTA is added in pending sewage containing ammonia, mineral matter dissolving mixes Mixed liquor is obtained, then the ratio addition trace element solution mixing of 1.0~1.5mL trace element solutions is added in every liter of mixed liquor Obtain sewage disinfection treatment liquid;
2) add and detest after the liquid for half volume of the mud mixture obtained after foregoing enrichment culture Feammox microorganisms being drained Sewage disposal is carried out in oxygen reactor:Add in the reactor pre- with the foregoing sewage of mud mixture same volume in reactor Discharge and the sewage disinfection treatment liquid phase same volume added after treatment fluid operation 210min and then precipitation 18min out of service, precipitation Solution and collect the liquid, so far complete a process cycle;Again in reactor add with displaced volume same volume Long-pending sewage disinfection treatment liquid, start a new process cycle, so circulation can be continued for sewage disposal;Reactor Running temperature is 30~35 DEG C, and the mud mixture pH in reactor is controlled 7.0~8.0;Arranged once a day out of reactor Go out reacted mud mixture cumulative volume 1/15th abandons, and then fills into the sewage with discharge liquor same volume again Pretreatment fluid;So far complete the NH in sewage4 +It is converted into NO2 Process;
3rd, the liquid being collected into after foregoing anaerobism Feammox processing is subjected to denitrogenation processing.
2. the biological denitrification method as claimed in claim 1 based on anaerobic ferrite oxidization ammonia, it is characterised in that in the nutrient solution Fe (III) NTA concentration be 0.18mM, NH4 +Concentration be 2.15mM, Fe (III) NTA's in the sewage disinfection treatment liquid Concentration is 0.18mM.
3. the biological denitrification method as claimed in claim 1 based on anaerobic ferrite oxidization ammonia, it is characterised in that described Fe (III) NTA is by Fe2(SO4)31 is pressed with NTA:1 mol ratio is matched somebody with somebody, described NH4 +From ammonium sulfate or ammonium chloride.
4. the biological denitrification method as claimed in claim 1 based on anaerobic ferrite oxidization ammonia, it is characterised in that the nutrient solution and The mineral component and proportioning included in the sewage disinfection treatment liquid be:NaH2PO40.04~0.06g/L, CaCl2·2H2O 0.2~0.4g/L, MgSO4·7H20.2~0.4g/L of O, KHCO31.0~1.5g/L, FeSO40.0060~0.0065g/L, 0.0060~0.0065g/L of EDTA;Described trace element solution includes the component of following concentration:13~17g/L of EDTA, H3BO40.010~0.016g/L, MnCl2·4H20.90~1.10g/L of O, CuSO4·5H20.20~0.30g/L of O, ZnSO4·7H20.35~0.50g/L of O, NiCl2·6H20.15~.025g/L of O, Na2SeO4·10H20.15~0.26g/ of O L、Na2MoO4·2H20.17~0.27g/L of O, Na2WO4·2H20.03~0.07g/L of O.
5. the biological denitrification method as claimed in claim 4 based on anaerobic ferrite oxidization ammonia, it is characterised in that the nutrient solution and The mineral component and proportioning included in the sewage disinfection treatment liquid be:NaH2PO4 0.05g/L、CaCl2·2H2O 0.3g/ L、MgSO4·7H2O 0.3g/L、KHCO3 1.25g/L、FeSO40.00625g/L、EDTA 0.00625g/L;The micro member Plain solution includes the component of following concentration:EDTA 15g/L、H3BO4 0.014g/L、MnCl2·4H2O 0.99g/L、CuSO4· 5H2O 0.25g/L、ZnSO4·7H2O 0.43g/L、NiCl2·6H2O 0.19g/L、Na2SeO4·10H2O 0.21g/L、 Na2MoO4·2H2O 0.22g/L、Na2WO4·2H2O 0.05g/L。
6. the biological denitrification method as claimed in claim 1 based on anaerobic ferrite oxidization ammonia, it is characterised in that described by anaerobism The method that the liquid being collected into after Feammox processing carries out denitrogenation processing is anaerobic ammonia oxidation process processing, by the NO in solution2 It is converted into N2
7. the biological denitrification method as claimed in claim 1 based on anaerobic ferrite oxidization ammonia, it is characterised in that the trace element Solution addition is:1.25mL trace element solutions are added in every liter of nutrient solution, every liter of mixed liquor adds during sewage disinfection treatment 1.25mL trace element solution.
8. the biological denitrification method as claimed in claim 1 based on anaerobic ferrite oxidization ammonia, it is characterised in that the fortune in reactor Trip temperature is 30~32 DEG C, and the mud mixture pH in reactor is controlled 7.0~7.5.
9. the biological denitrification method based on anaerobic ferrite oxidization ammonia as any one of claim 1 to 8 is in sewage water denitrification Application in processing.
CN201510775812.8A 2015-11-13 2015-11-13 Biological denitrification method and its application based on anaerobic ferrite oxidization ammonia Active CN105271514B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510775812.8A CN105271514B (en) 2015-11-13 2015-11-13 Biological denitrification method and its application based on anaerobic ferrite oxidization ammonia

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510775812.8A CN105271514B (en) 2015-11-13 2015-11-13 Biological denitrification method and its application based on anaerobic ferrite oxidization ammonia

Publications (2)

Publication Number Publication Date
CN105271514A CN105271514A (en) 2016-01-27
CN105271514B true CN105271514B (en) 2018-03-23

Family

ID=55141452

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510775812.8A Active CN105271514B (en) 2015-11-13 2015-11-13 Biological denitrification method and its application based on anaerobic ferrite oxidization ammonia

Country Status (1)

Country Link
CN (1) CN105271514B (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105923764A (en) * 2016-06-27 2016-09-07 南京大学 Method for quickly starting anaerobic ammonia oxidation technology by manganese dioxide
DK3484996T3 (en) * 2016-07-14 2020-12-07 Basf Se FERMENTATION MEDIUM INCLUDING A CHELATING AGENT
CN105948280A (en) * 2016-07-22 2016-09-21 中国环境科学研究院 Anaerobic biological oxidation water pollution remediation method with Fe3+ in hematite as electron acceptor
CN107555616B (en) * 2017-10-31 2020-05-12 苏州科技大学 Method for synchronously removing ammonia nitrogen and nitrate nitrogen in water body
CN109650536A (en) * 2019-01-03 2019-04-19 大连理工大学 The device and method of ammonia nitrogen anaerobism in-situ treatment based on ferrikinetics driving

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103803703A (en) * 2014-02-25 2014-05-21 合肥工业大学 Method for simultaneously removing phosphorous and nitrogen through synergistic effect of nanoscale-iron and microbes

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4784873B2 (en) * 2007-04-13 2011-10-05 独立行政法人産業技術総合研究所 Anaerobic ammonia oxidation treatment method and apparatus

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103803703A (en) * 2014-02-25 2014-05-21 合肥工业大学 Method for simultaneously removing phosphorous and nitrogen through synergistic effect of nanoscale-iron and microbes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Possibility of anoxic ferric ammonium oxidation;Sawayama, S;《Journal of Bioscience and Bioengineering》;20060131;第101卷(第1期);第70-72页 *
厌氧氨氧化微生物利用不同电子受体的可行性研究;刘福鑫;《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》;20150315;第17-18页 *
活性污泥异化铁还原协同脱氮除磷研究;冯娟娟;《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》;20140215;第20页第2.1.1节,第34页第3.1节,第42-44页第4.1节,第51-53页第4.2.6节 *

Also Published As

Publication number Publication date
CN105271514A (en) 2016-01-27

Similar Documents

Publication Publication Date Title
Batstone et al. Mathematical modelling of anaerobic digestion processes: applications and future needs
Zhi et al. Enhanced long-term nitrogen removal and its quantitative molecular mechanism in tidal flow constructed wetlands
Ding et al. Nitrogen loss through anaerobic ammonium oxidation coupled to iron reduction from paddy soils in a chronosequence
Picardal Abiotic and microbial interactions during anaerobic transformations of Fe (II) and NOx
Nivala et al. Oxygen transfer and consumption in subsurface flow treatment wetlands
Wang et al. Side-stream sludge treatment using free nitrous acid selectively eliminates nitrite oxidizing bacteria and achieves the nitrite pathway
Law et al. Producing free nitrous acid–A green and renewable biocidal agent–From anaerobic digester liquor
Gujer Nitrification and me–A subjective review
Pena-Castro et al. Heavy metals removal by the microalga Scenedesmus incrassatulus in continuous cultures
Berge et al. The fate of nitrogen in bioreactor landfills
Fijałkowski et al. The influence of selected soil parameters on the mobility of heavy metals in soils
Feleke et al. A bio-electrochemical reactor coupled with adsorber for the removal of nitrate and inhibitory pesticide
Kalin et al. The chemistry of conventional and alternative treatment systems for the neutralization of acid mine drainage
Gazea et al. A review of passive systems for the treatment of acid mine drainage
Park et al. Empirical model of the pH dependence of the maximum specific nitrification rate
Ortner et al. Can bioavailability of trace nutrients be measured in anaerobic digestion?
Yu et al. Iron redox cycling coupled to transformation and immobilization of heavy metals: implications for paddy rice safety in the red soil of South China
Mejia et al. Influence of oxygen and nitrate on Fe (Hydr) oxide mineral transformation and soil microbial communities during redox cycling
Bai et al. Treatment of acid mine drainage by sulfate reducing bacteria with iron in bench scale runs
Kirby et al. Relative contributions of abiotic and biological factors in Fe (II) oxidation in mine drainage
Ratering et al. Nitrate‐dependent iron (II) oxidation in paddy soil
Yetilmezsoy et al. Development of empirical models for performance evaluation of UASB reactors treating poultry manure wastewater under different operational conditions
CN101484394B (en) A method for removing the contamination of c, n utilizing heterotrophic ammonia-oxidizing bacteria
Faulwetter et al. Microbial processes influencing performance of treatment wetlands: a review
CN103626293B (en) A kind of natural magnetic iron ore biofilter and utilize its synchronous method of removing nitre nitrogen and phosphorus in water

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant