CN105175140A - Culture medium for cultivating coprinus comatus - Google Patents
Culture medium for cultivating coprinus comatus Download PDFInfo
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- CN105175140A CN105175140A CN201510591570.7A CN201510591570A CN105175140A CN 105175140 A CN105175140 A CN 105175140A CN 201510591570 A CN201510591570 A CN 201510591570A CN 105175140 A CN105175140 A CN 105175140A
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- coprinus comatus
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Abstract
The invention discloses a culture medium for cultivating coprinus comatus and belongs to the technical field of plant cultivation. The culture medium which is low in cost and applicable to growth of the coprinus comatus comprises the following components in parts by weight: 15-25 parts of corncob, 7-20 parts of cotton seed hull, 5-15 parts of weed tree sawdust, 7-20 parts of wheat bran, 7-20 parts of soybean meal, 7-21 parts of cane sugar, 20-30 parts of loamy soil, 5-11 parts of quicklime, 2-7 parts of horse manure, 0.5-1 part of phosphate fertilizer, 0.2-2 parts of urea, 1-4 parts of carrot, 0.5-2 parts of magnesium sulphate, 0.1-2 parts of thiophanate methyl, 0.5-3 parts of a defoaming agent and 70-150 parts of distilled water. The coprinus comatus obtained through cultivation by virtue of the culture medium prepared from the components in proportion grows fast and is good in taste and balanced in nutrition.
Description
Technical field
The present invention discloses a kind of cultivation matrix being applicable to sending out scale cultivating drumstick mushroom, and it belongs to the technical field of plant cultivation media.
Background technology
Coprinus comatus has another name called Shaggy Mane, hair ghost umbrella, thorn mushroom, belongs to Mycophyta, Basidiomycotina, Hymenomycetes, Agaricales, Gui San section.Fine and tender taste during coprinus comatus children, tasty, color, smell and taste are neither second to straw mushroom.Coprinus comatus or a kind of Medicinal Mushroom, the sweet property of taste is put down, and useful taste, clearing away the heart fire and tranquillizing, controls the effects such as hemorrhoid, often edible have aid digestion, improve a poor appetite and treat the effect of hemorrhoid." Chinese medicinal fungi illustrated handbook " is recorded, and its hot water extract is respectively 100% and 90% to small white mouse sarcoma 180 and ehrlich carcinoma inhibiting rate.Coprinus comatus contains treatment diabetes effective constituent.The U.S., Holland, France, Germany, Italy, Japan cultivate successfully in succession in recent years.China scientific and technical personnel carry out investigating, gather, are separated and experiment in cultivation, have very considerable production & marketing potentiality.
Summary of the invention
The invention provides a kind of substratum of suitable Coprinus comatus growth, it consists of: corn cob, cotton seed hulls, weed tree sawdust, wheat bran, bean cake powder, sucrose, loam, unslaked lime, horsehit, phosphate fertilizer, urea, Radix Dauci Sativae, magnesium sulfate, thiophanate methyl, defoamer, distilled water.
Particularly, the weight part of above-mentioned each component is: corn cob 15 ~ 25 parts, cotton seed hulls 7 ~ 20 parts, weed tree sawdust 5 ~ 15 parts, 7 ~ 20 parts, wheat bran, bean cake powder 7 ~ 20 parts, sucrose 7 ~ 21 parts, 20 ~ 30 parts, loam, unslaked lime 5 ~ 11 parts, horsehit 2 ~ 7 parts, 0.5 ~ 1 part, phosphate fertilizer, 0.2 ~ 2 part, urea, 1 ~ 4 part, Radix Dauci Sativae, 0.5 ~ 2 part, magnesium sulfate, thiophanate methyl 0.1 ~ 2 part, defoamer 0.5 ~ 3 part, distilled water 70 ~ 150 parts.
Further, the weight part of above-mentioned each component is: corn cob 17 ~ 22 parts, cotton seed hulls 8 ~ 18 parts, weed tree sawdust 6 ~ 13 parts, 8 ~ 17 parts, wheat bran, bean cake powder 8 ~ 18 parts, sucrose 8 ~ 18 parts, 20 ~ 28 parts, loam, unslaked lime 6 ~ 9 parts, horsehit 4 parts, 0.5 ~ 1 part, phosphate fertilizer, 0.5 ~ 1.5 part, urea, 3 parts, Radix Dauci Sativae, 1 part, magnesium sulfate, thiophanate methyl 0.5 part, defoamer 2 parts, distilled water 70 ~ 150 parts.
Further, the weight part of above-mentioned each component is: corn cob 18 ~ 20 parts, cotton seed hulls 10 ~ 18 parts, weed tree sawdust 7 ~ 12 parts, 9 ~ 16 parts, wheat bran, bean cake powder 10 ~ 18 parts, sucrose 10 ~ 18 parts, 28 parts, loam, unslaked lime 7 parts, horsehit 3 parts, 0.8 part, phosphate fertilizer, 1 part, urea, 3 parts, Radix Dauci Sativae, 1.5 parts, magnesium sulfate, thiophanate methyl 0.5 part, defoamer 2 parts, distilled water 70 ~ 150 parts.
Particularly, the above-mentioned distilled water that adds makes the water content of culture material be 60% ~ 70%.
Particularly, in said components, add acid-base modifier makes the pH of substratum be 6 ~ 7.
Need to add soil in the medium to stimulate the stimulation of soil microorganisms meta-bolites etc., the soil wherein added is loam.
Cotton seed hulls also claims cotton skin, is the shell that cottonseed is left after decorticator is separated.Different according to the type of machinery of peeling off, cotton seed kind different, the place of production is different, water content is different, peel off after broken cotton benevolence powder degree of sieving not equal.The size of the cotton seed hulls processed, color, velveteen length, nutrition (containing cotton benevolence powder) are also different.Cotton seed hulls is mainly used in: cultivate edible medicinal fungus, as feed, raise livestock etc.
Thiophanate_methyl trade(brand)name thiophanate methyl, is inhale low toxicity sterilant in a kind of broad spectrum, has interior suction, preventive and therapeutic action.
Full name phosphorus fertilizer; Phosphate fertilizer take phosphorus as the fertilizer of Main Nutrients, the size (significance degree) of p fertilizer efficiency and speed be decided by effective Vanadium Pentoxide in FLAKES in phosphate fertilizer content, soil property, put fertile method, crop species etc.The phosphate fertilizer added in the present invention is water soluble phosphorous fertilizer, as normal superphosphate, double superhosphate etc.Its main component is mono-calcium phosphate.Soluble in water, fertilizer efficiency is very fast.
Urea, also known as carboxamide (carbamide), is a kind of white crystal.One of the simplest organic compound.The diamide of carbonic acid, molecular formula is H
2nCONH
2(CO (NH
2)
2).The main nitrogenous end product of Mammals and some Fish internal protein metabolic breakdown.Also be the nitrogenous fertilizer that current nitrogen content is the highest.As a kind of neutral fertilizer, urea is applicable to various soil and plant.It is easily preserved, easy to use, little to the destruction of soil, is a kind of chemical nitrogen fertilizer that current usage quantity is larger.Industrial ammonia and carbonic acid gas urea synthesis under certain condition.
In the present invention, lime is Powdered.
In horsehit, content of cellulose is high, and quality is slightly loose, and containing more high temperature fiber decomposer, moisture is few, and the heat discharged in digest process is many, therefore has the title of hot manure.The base manure that the horsehit become thoroughly decomposed can do vegetables is used, and also can do the heating material of Greenhouse for cultivation of seedling in early spring.
Dregs of beans is owing to needing first oil and grease extracting, what therefore it was chosen mostly is the genetically engineered soybean that lipid content is higher, after extraction process, fatty residual quantity mostly≤0.5%, as the bean powder that raw material makes, fineness can reach 80 orders, 100 orders, 120 orders even more (producer had claims can provide 200 order bean powderes), but this bean powder has a kind of feature, because its lipid content is very low, so in fermentative production, must be aided with a large amount of foam killers, otherwise foam is uncontrollable.
Containing abundant carotene, sugar, iron, VITMAIN B1, Lin Suanna Vitamin B2 Sodium Phosphate, vitamins C and pectin, starch, inorganic salt and multiple amino acids in Radix Dauci Sativae.。
Magnesium sulfate, magnesium sulfate is that solubleness is higher compared with the advantage of other fertilizer.Magnesium sulfate is also used as bath salt.
Loam, refers to the soil that glutinous grain in soil particle composition, powder, sand grain content are moderate.Quality, between clay and sand, has the advantage of clay and sand concurrently, and venting and water permeable, water conservation heat-insulating property are all better, are comparatively ideal agricultural soils.
Coprinus comatus is the rotten autochthonal bacterium of the extremely strong grass of a kind of adaptive faculty, and its living condition requires as follows: (1) nutrition: coprinus comatus can utilize carbon source quite widely.Glucose, wood sugar, semi-lactosi, maltose, raffinose, N.F,USP MANNITOL, starch, Mierocrystalline cellulose, paraffin wax can utilize.Utilize wood sugar poorer than glucose, utilize lactose fairly good, but be not best; Some bacterial strain utilizes semi-lactosi and lactose to be better than and utilizes N.F,USP MANNITOL, glucose, fructose; Utilize sunshine ability poor.
Peptone and yeast powder are the best nitrogenous sources of coprinus comatus.Coprinus comatus can utilize various ammonium salt and nitric nitrogen, but inorganic nitrogen and urea are not the suitableeest nitrogenous sources, and in malt extract medium, add asparagine, peptone, urea, mycelial growth is better.
The culture vessel of making method cultivar adopts polyacrylic film bag (long 34 ~ 36 centimetres, wide 14 ~ 17 centimetres, thickness 0.05 ~ 0.06 centimetre).First grab 2 ~ 3 during pack culture material is put in bag, with hand in the corner press-in bag at the bottom of bag, and compress culture material and make it into cylindrical, at the bottom of bag, steadily can stand on ground.In bag, insert the test tube of circular wooden stick or diameter 2 ~ 2.5 centimetres, be preferably inserted into the end, but should avoid puncturing sack, then continue charging limit, limit hand real, be filled to 2/3 (the about 500 grams of siccatives) that bag is long, flatten table and, extract wooden stick or test tube.With the vapor pressure sterilizing 1.5 hours of 1.5 kg/cm, or atmospheric steam sterilizing 8 ~ 10 hours, take out cooling after sterilizing.
Culture medium of the present invention, the Coprinus comatus mouthfeel that the balanced in nutrition last cultivation due to each component obtains is good.
Specific embodiment
Embodiment 1
Corn cob 15 parts, cotton seed hulls 15 parts, weed tree sawdust 10 parts, 15 parts, wheat bran, bean cake powder 12 parts, sucrose 18 parts, 30 parts, loam, unslaked lime 8 parts, horsehit 2 parts, 0.5 part, phosphate fertilizer, 1 part, urea, 2.5 parts, Radix Dauci Sativae, 1.5 parts, magnesium sulfate, thiophanate methyl 0.5 part, defoamer 2 parts, distilled water 125 parts.
Embodiment 2
Corn cob 15 parts, cotton seed hulls 8 parts, weed tree sawdust 6 parts, 18 parts, wheat bran, bean cake powder 15 parts, sucrose 20 parts, 25 parts, loam, unslaked lime 6 parts, horsehit 5 parts, 1 part, phosphate fertilizer, 1.2 parts, urea, 3 parts, Radix Dauci Sativae, 1 part, magnesium sulfate, thiophanate methyl 0.5 part, defoamer 2 parts, distilled water 150 parts.
Embodiment 3
Corn cob 25 parts, cotton seed hulls 18 parts, weed tree sawdust 13 parts, 18 parts, wheat bran, bean cake powder 15 parts, sucrose 15 parts, 28 parts, loam, unslaked lime 8 parts, horsehit 6 parts, 0.6 part, phosphate fertilizer, 0.8 part, urea, 4 parts, Radix Dauci Sativae, 1.5 parts, magnesium sulfate, thiophanate methyl 0.4 part, defoamer 1.8 parts, distilled water 145 parts.
The substratum be made up of proportioning, the mouthfeel of finally cultivating the Coprinus comatus obtained is good, nutritious, and wherein the growth velocity of Coprinus comatus is 1.5 ~ 2 times of ordinary culture medium growth velocity.Substratum simultaneously after above-mentioned cultivation adds after a small amount of trophoplasm again can cultivating drumstick mushroom again.
Claims (4)
1. cultivate a substratum for Coprinus comatus, it consists of: corn cob, cotton seed hulls, weed tree sawdust, wheat bran, bean cake powder, sucrose, loam, unslaked lime, horsehit, phosphate fertilizer, urea, Radix Dauci Sativae, magnesium sulfate, thiophanate methyl, defoamer, distilled water.
2. a kind of substratum cultivating Coprinus comatus according to claim 1, is characterized in that: the weight part of described each component is: corn cob 15 ~ 25 parts, cotton seed hulls 7 ~ 20 parts, weed tree sawdust 5 ~ 15 parts, 7 ~ 20 parts, wheat bran, bean cake powder 7 ~ 20 parts, sucrose 7 ~ 21 parts, 20 ~ 30 parts, loam, unslaked lime 5 ~ 11 parts, horsehit 2 ~ 7 parts, 0.5 ~ 1 part, phosphate fertilizer, 0.2 ~ 2 part, urea, 1 ~ 4 part, Radix Dauci Sativae, 0.5 ~ 2 part, magnesium sulfate, thiophanate methyl 0.1 ~ 2 part, defoamer 0.5 ~ 3 part, distilled water 70 ~ 150 parts.
3. a kind of substratum cultivating Coprinus comatus according to claim 1, is characterized in that: the weight part of described each component is: corn cob 17 ~ 22 parts, cotton seed hulls 8 ~ 18 parts, weed tree sawdust 6 ~ 13 parts, 8 ~ 17 parts, wheat bran, bean cake powder 8 ~ 18 parts, sucrose 8 ~ 18 parts, 20 ~ 28 parts, loam, unslaked lime 6 ~ 9 parts, horsehit 4 parts, 0.5 ~ 1 part, phosphate fertilizer, 0.5 ~ 1.5 part, urea, 3 parts, Radix Dauci Sativae, 1 part, magnesium sulfate, thiophanate methyl 0.5 part, defoamer 2 parts, distilled water 70 ~ 150 parts.
4. a kind of substratum cultivating Coprinus comatus according to claim 1, is characterized in that: the weight part of described each component is: corn cob 18 ~ 20 parts, cotton seed hulls 10 ~ 18 parts, weed tree sawdust 7 ~ 12 parts, 9 ~ 16 parts, wheat bran, bean cake powder 10 ~ 18 parts, sucrose 10 ~ 18 parts, 28 parts, loam, unslaked lime 7 parts, horsehit 3 parts, 0.8 part, phosphate fertilizer, 1 part, urea, 3 parts, Radix Dauci Sativae, 1.5 parts, magnesium sulfate, thiophanate methyl 0.5 part, defoamer 2 parts, distilled water 70 ~ 150 parts.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510591570.7A CN105175140A (en) | 2015-09-17 | 2015-09-17 | Culture medium for cultivating coprinus comatus |
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| CN201510591570.7A CN105175140A (en) | 2015-09-17 | 2015-09-17 | Culture medium for cultivating coprinus comatus |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105622216A (en) * | 2016-04-07 | 2016-06-01 | 于如龙 | Novel edible fungus culture medium and culture method |
| CN107711283A (en) * | 2017-09-28 | 2018-02-23 | 广西浙缘农业科技有限公司 | A kind of cultural method of coprinus comatus |
| CN109122021A (en) * | 2018-08-23 | 2019-01-04 | 安徽农耕年华农业发展有限公司 | A kind of mushroom cultivating method |
| CN113273433A (en) * | 2021-06-04 | 2021-08-20 | 湖南省林业科学院 | Coprinus comatus culture medium taking oil-tea camellia cake as raw material and preparation method thereof |
-
2015
- 2015-09-17 CN CN201510591570.7A patent/CN105175140A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105622216A (en) * | 2016-04-07 | 2016-06-01 | 于如龙 | Novel edible fungus culture medium and culture method |
| CN107711283A (en) * | 2017-09-28 | 2018-02-23 | 广西浙缘农业科技有限公司 | A kind of cultural method of coprinus comatus |
| CN109122021A (en) * | 2018-08-23 | 2019-01-04 | 安徽农耕年华农业发展有限公司 | A kind of mushroom cultivating method |
| CN113273433A (en) * | 2021-06-04 | 2021-08-20 | 湖南省林业科学院 | Coprinus comatus culture medium taking oil-tea camellia cake as raw material and preparation method thereof |
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Application publication date: 20151223 |
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| WD01 | Invention patent application deemed withdrawn after publication |