CN105154330A - Centrifugal loader for cell high acceleration loading in horizontal direction - Google Patents
Centrifugal loader for cell high acceleration loading in horizontal direction Download PDFInfo
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- CN105154330A CN105154330A CN201510571988.1A CN201510571988A CN105154330A CN 105154330 A CN105154330 A CN 105154330A CN 201510571988 A CN201510571988 A CN 201510571988A CN 105154330 A CN105154330 A CN 105154330A
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- rotor
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- whizzer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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Abstract
A centrifugal loader for cell high acceleration loading in a horizontal direction comprises a centrifugal machine body and a rotor system, wherein the centrifugal machine body comprises a centrifugal machine shell, a machine cover, a rotary motor and a control panel, the centrifugal machine shell is a closed box body formed by a centrifugal machine inner case and a centrifugal machine outer case; the rotor system consists of a rotating shaft, a rotor body and a rotor cover, the rotating shaft is connected with a motor shaft through a flange, the rotor body is connected with the rotating shaft through a withdrawal nut, and the rotor cover is fixed onto the rotor body through tightening a rotor lifting handle. When the centrifugal loader is used, culture plates and culture bottles are respectively placed in corresponding grooves in the rotor body for performing a loading test. The centrifugal loader provided by the invention has the beneficial effects that the cell centrifugal loader is based on a centrifugal machine, and through setting rotor speed and lifting speed acceleration parameters, the constant acceleration centrifugal loading or variable acceleration loading of a cell in the horizontal direction are realized; the centrifugal loader is simple and compact in structure, and the culture and loading of the cell are convenient.
Description
Technical field
Patent of the present invention relates to biomedical engineering technology field, particularly a kind of load for cells in vitro horizontal direction high acceleration centrifugally add carrier aircraft.
Background technology
Along with development that is scientific and technological and modern aerospace cause, the maneuvering characteristics of air fighter is very significantly improved, awing high-performance fighter aircraft can produce up to acceleration more than+9Gz, the health of this extreme mechanical environment to pilot has very major injury, has become one of principal element threatening pilot's health.Investigation display, through selecting healthy pilot layer by layer, after being engaged in working flight for some time, crowd is high than usual for their ARR incidence.Research also shows, Space Special Environment also has certain influence to human body, can cause human body bone loss, amyotrophy and endocrine dysfunction etc.And at space shuttle in lift-off and return course, especially in the emergent return stage, spacefarer can be subject to high positive acceleration effect, by carrying out high positive acceleration experimental study to monkey, find that high positive acceleration can produce obvious pathology damage to tissues such as the lungs of monkey, kidneys, so the Mechanobiology impact of high acceleration more and more comes into one's own.
At cell levels, scholar finds that carrying out different positive acceleration (+Gz) exposes and load different time, there is impact in various degree to rat brain neurone, during low G value, rat cerebral cortex neuron linear mitochondrial structure can be caused to damage in the short period of time (3min); Research also finds to carry out 3 times and loads 12G(+Gz), after exposing 3min, Rat Astroglia activates, GFAP(glial fibrillary acidic protein) up-regulated, so high acceleration also has a certain impact to cell.
Centrifugal high acceleration deceleration loading device is the instrument that current experimental study high acceleration mechanical environment is conventional, it is placed in indoor, good work environment, take up room little, dynamic process controllable precise, emulation mode can repeatedly accurately repeat, permission long-time continuous is run, observe easy to detect, suitability is strong, is the first-selected device of research cell mechanical response under centrifugal high acceleration loading environment.In order to study the impact of high acceleration on cell, probe into the Response Mechanism of cell under high acceleration, we devise a kind of cell centrifugation formula and add carrier aircraft, load for the centrifugal high acceleration in cell levels direction.
Summary of the invention
The object of the invention is for above-mentioned technical Analysis, there is provided a kind of and add carrier aircraft for the centrifugal of cell levels direction high acceleration loading, this is centrifugal, and to add carrier aircraft simple and compact for structure, the culture plate adopting laboratory culture cell to commonly use and culturing bottle are container, drive rotor to rotate by rotating machine to load the centrifugal high acceleration in cell levels direction, and, change parameters and can realize varying acceleration loading.
Technical scheme of the present invention:
A kind of load for cell levels direction high acceleration centrifugally add carrier aircraft, comprise whizzer body and rotor-support-foundation system, whizzer body comprises cover and whizzer housing, the enclosed box body that whizzer housing is made up of whizzer inner casing and whizzer shell, whizzer shell is equipped with control panel, cover and whizzer housing and pass through gemel connection; Rotor-support-foundation system is made up of rotating machine, motor shaft, turning axle, rotor block and rotor cover, rotating machine is arranged at the enclosed chamber inner bottom part center that whizzer inner casing and whizzer shell form, motor shaft is connected with turning axle by flange, turning axle top is provided with screw thread, rotor block is connected with turning axle by dismounting nut and is provided with back-up ring, rotor cover is fixed on rotor block by rotor handle and turning axle thread fit, and rotor block is balanced rotation under turning axle drives; Rectangle and circle connected in star is provided with for placing rectangle culture plate and circular culturing bottle in rotor block, nutrient solution is filled to ensure that cell is in all the time in nutrient solution in loading procedure between two pieces of culture plates He in culturing bottle during loading, clamp with clamping plate after sealing between two pieces of culture plates, culturing bottle utilizes cultivation bottle cap to tighten sealing, and during loading, the balance of rotor block by pasting silica-gel plate adjustment on culture plate or culturing bottle.
Principle of work of the present invention:
This centrifugal carrier aircraft that adds is a kind of for the centrifugal high acceleration deceleration loading device in cell levels direction, can provide rotating centrifugal effect to cell.Rotor block is used for clamping and fixes culture plate and culturing bottle, and when rotor cover guarantees that rotor operates, culture plate and culturing bottle can not fly out, and ensures security when running.By the rotating speed of control panel input setting motor, spin motor shaft driven rotary axle and rotor, realize the centrifugal loading of the horizontal direction of cell.The acceleration of raising speed and reduction of speed is set, varying acceleration loading can be carried out to cell.
The invention has the beneficial effects as follows: this cell centrifugation formula adds carrier aircraft based on whizzer, by arranging rotor speed and lifting speed acceleration parameter, realizing the cell centrifugal loading of constant acceleration or varying acceleration loading in the horizontal direction; This is centrifugal, and to add carrier aircraft simple and compact for structure, facilitates cultivation and the loading of cell.
Accompanying drawing explanation
Fig. 1 adds carrier aircraft structural representation for this is centrifugal.
Fig. 2 adds carrier aircraft internal structure schematic diagram for this is centrifugal.
Fig. 3 adds carrier aircraft rotating part structure enlarged diagram for this is centrifugal.
Fig. 4 is this centrifugal loading machine rotor partial enlargement structural representation.
In figure: 1. cover 2. whizzer inner casing 3. whizzer shell 4. rotor handle 5. rotor cover 6. rotor block 7. control panel 8. turning axle 9. bearing (setting) plate 10. bearing 11. flange 12. rotating machine 13. nut 14. bolt 15. motor shaft 16. dismantles nut 17. hole back-up ring 18. clamping plate 19. culture plate 20. culturing bottle.
Embodiment
Embodiment:
A kind of load for the centrifugal high acceleration of cell injuring model centrifugally add carrier aircraft, comprise whizzer body and rotor-support-foundation system, whizzer body comprises cover 1 and whizzer housing, the enclosed box body that whizzer housing is made up of whizzer inner casing 2 and whizzer shell 3, whizzer shell 3 is equipped with control panel 7, cover 1 and whizzer housing and pass through gemel connection; Rotor-support-foundation system is made up of rotating machine 12, motor shaft 15, turning axle 8, rotor block 6 and rotor cover 5, rotating machine 12 is arranged at the enclosed chamber inner bottom part center that whizzer inner casing 2 forms with whizzer shell 3, motor shaft 15 is connected with turning axle 8 by flange 11, turning axle 8 top is provided with screw thread, rotor block 6 is connected with turning axle 8 by dismounting nut 16 and is provided with back-up ring 17, rotor cover 5 is fixed on rotor block 6 by rotor handle 4 and turning axle 8 thread fit, and rotor block 6 is balanced rotation under turning axle 8 drives; Rectangle and circle connected in star is provided with for placing rectangle culture plate 19 and circular culturing bottle 20 in rotor block 6, nutrient solution is filled to ensure that cell is in all the time in nutrient solution in loading procedure between two pieces of culture plates 19 He in culturing bottle 20 during loading, clamp with clamping plate 18 after sealing between two pieces of culture plates 19, culturing bottle 20 utilizes cultivation bottle cap to tighten sealing, and during loading, the balance of rotor block 6 by pasting silica-gel plate adjustment on culture plate 19 or culturing bottle 20.
After rotor block 6 machines, carry out balance test, ensure the stability of rotor operation.During use, culture plate 19 and culturing bottle 20 are placed in the groove of rotor block 6 correspondence respectively, tighten rotor handle and rotor cover 5 is fixed on rotor block 6, the security of culture plate 19 and culturing bottle 20 when rotor cover 5 can ensure that rotor operates.When loading culture plate 19 and culturing bottle 20, need to fill nutrient solution in culture plate 19 and culturing bottle 20, this can ensure that cell is in all the time in nutrient solution in loading procedure.There will not be leakage in order to ensure the nutrient solution in culture plate in loading procedure 19, will sealing be carried out after culture plate 19 fills nutrient solution, and then with clamping plate 18, culture plate and culture plate lid are clamped.And cultivation bottle cap only need be tightened by culturing bottle 20 after filling nutrient solution, nutrient solution would not flow out.Culture plate 19 after clamping with clamping plate 18 and culturing bottle 20 fill nutrient solution after need to do Balance Treatment, two culturing bottles 20 or culture plate 19 are placed on respectively on the plate of balance instrument both sides, silica-gel plate is pasted toward light side, until balance, then additional silica-gel plate is pasted onto on corresponding culture plate 19 or culturing bottle 20, after guaranteeing two lateral balances, then culture plate 19 or culturing bottle 20 are put into groove corresponding to rotor, carry out load test.
Claims (1)
1. one kind adds carrier aircraft for the centrifugal of cell levels direction high acceleration loading, it is characterized in that: comprise whizzer body and rotor-support-foundation system, whizzer body comprises cover and whizzer housing, the enclosed box body that whizzer housing is made up of whizzer inner casing and whizzer shell, whizzer shell is equipped with control panel, cover and whizzer housing and pass through gemel connection; Rotor-support-foundation system is made up of rotating machine, motor shaft, turning axle, rotor block and rotor cover, rotating machine is arranged at the enclosed chamber inner bottom part center that whizzer inner casing and whizzer shell form, motor shaft is connected with turning axle by flange, turning axle top is provided with screw thread, rotor block is connected with turning axle by dismounting nut and is provided with back-up ring, rotor cover is fixed on rotor block by rotor handle and turning axle thread fit, and rotor block is balanced rotation under turning axle drives; Rectangle and circle connected in star is provided with for placing rectangle culture plate and circular culturing bottle in rotor block, nutrient solution is filled to ensure that cell is in all the time in nutrient solution in loading procedure between two pieces of culture plates He in culturing bottle during loading, clamp with clamping plate after sealing between two pieces of culture plates, culturing bottle utilizes cultivation bottle cap to tighten sealing, and during loading, the balance of rotor block by pasting silica-gel plate adjustment on culture plate or culturing bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510571988.1A CN105154330A (en) | 2015-09-09 | 2015-09-09 | Centrifugal loader for cell high acceleration loading in horizontal direction |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510571988.1A CN105154330A (en) | 2015-09-09 | 2015-09-09 | Centrifugal loader for cell high acceleration loading in horizontal direction |
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| CN105154330A true CN105154330A (en) | 2015-12-16 |
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| CN201510571988.1A Pending CN105154330A (en) | 2015-09-09 | 2015-09-09 | Centrifugal loader for cell high acceleration loading in horizontal direction |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109022340A (en) * | 2018-06-28 | 2018-12-18 | 江苏云宇医疗科技有限公司 | A kind of cell centrifugal method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4449965A (en) * | 1982-10-04 | 1984-05-22 | Beckman Instruments, Inc. | Shell type centrifuge rotor having controlled windage |
| JP2003230849A (en) * | 2003-03-14 | 2003-08-19 | Hitachi Koki Co Ltd | Centrifugal separator and method |
| CN203140171U (en) * | 2013-01-29 | 2013-08-21 | 珠海黑马医学仪器有限公司 | Centrifuge |
| CN203518430U (en) * | 2013-08-02 | 2014-04-02 | 中山康方生物医药有限公司 | Elisa plate spin dryer |
| CN204396188U (en) * | 2014-12-22 | 2015-06-17 | 南华大学 | A kind of vacuum refrigeration centrifuge |
| CN104789469A (en) * | 2015-04-29 | 2015-07-22 | 天津理工大学 | Bioreactor for in-vitro cell culture/animal centrifugal high-acceleration loading |
-
2015
- 2015-09-09 CN CN201510571988.1A patent/CN105154330A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4449965A (en) * | 1982-10-04 | 1984-05-22 | Beckman Instruments, Inc. | Shell type centrifuge rotor having controlled windage |
| JP2003230849A (en) * | 2003-03-14 | 2003-08-19 | Hitachi Koki Co Ltd | Centrifugal separator and method |
| CN203140171U (en) * | 2013-01-29 | 2013-08-21 | 珠海黑马医学仪器有限公司 | Centrifuge |
| CN203518430U (en) * | 2013-08-02 | 2014-04-02 | 中山康方生物医药有限公司 | Elisa plate spin dryer |
| CN204396188U (en) * | 2014-12-22 | 2015-06-17 | 南华大学 | A kind of vacuum refrigeration centrifuge |
| CN104789469A (en) * | 2015-04-29 | 2015-07-22 | 天津理工大学 | Bioreactor for in-vitro cell culture/animal centrifugal high-acceleration loading |
Non-Patent Citations (1)
| Title |
|---|
| 雷东锋: "《现代生物化学与分子生物学仪器与设备》", 31 January 2006 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109022340A (en) * | 2018-06-28 | 2018-12-18 | 江苏云宇医疗科技有限公司 | A kind of cell centrifugal method |
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Application publication date: 20151216 |