CN105143861A - Quantitative detection method of CRP using immune colloidal gold test plate and biochemical parameter photoelectric detector - Google Patents
Quantitative detection method of CRP using immune colloidal gold test plate and biochemical parameter photoelectric detector Download PDFInfo
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- CN105143861A CN105143861A CN201380075557.5A CN201380075557A CN105143861A CN 105143861 A CN105143861 A CN 105143861A CN 201380075557 A CN201380075557 A CN 201380075557A CN 105143861 A CN105143861 A CN 105143861A
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- strip plate
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
Abstract
A biochemical parameter photoelectric detector and a detection method of hypersensitive C-reactive protein. The detector comprises a photoelectric detection unit, a sensing circuit, a control and data processing circuit and a data output unit. The photoelectric detection unit comprises three or more emitting light sources and an optical receiver or comprises an emitting light source and three or more optical receivers, wherein the multiple emitting light sources are arranged to distribute symmetrically around the optical receiver or the multiple optical receivers are arranged to distribute symmetrically around the emitting light source, incident light rays emitted by the multiple emitting light sources or reflected light rays received by the multiple optical receivers form a 45-75 degrees included angle with a plane where a detection area of a test strip plate is located. The photoelectric detector is used for detecting a C-reactive protein immune colloidal gold test strip plate with a sample loaded, and concentration of the C-reactive protein is provided through fitting. The multiple emitting light sources or the multiple optical receivers that are symmetrically distributed achieve a location difference compensation effect, reduce interference brought by location differences of the test strip plate, and improve detection sensitivity.
Description
Immune colloid gold test plate (panel) and biochemical parameter photoelectric detector quantitatively detect CRP method and technology field
The invention belongs to Anti-TNF-α body technique, gold labeling antibody technology and photoelectric sensor technical field, the method that particularly a kind of immune colloid gold test paper and its photoelectric sensor are quantitatively detected, it is used especially for CRP detection.Background technology
Immune colloid gold analysis has that detection efficiency is high, method cylinder just, pollution-free, stable reagent the features such as, the extensive use in clinical examination field.But initial immunity colloidal gold test paper strip is only used in qualitative or sxemiquantitative tachysynthesis detection method, is limited by very large its clinical application range.
In first technology, it is the depth of the kermesinus shown by detection band and quality control band gone out in observation test strips by visual observation to the judgement that the checking matter on immunity colloidal gold test paper strip is added dropwise to realize.
Above-mentioned first technology mainly has following several shortcomings：
1. quantitative measurment is unable to, can only observational measurement.In gold-marking immunity test strips, detect that the colored depth reflects the height of checking matter concentration.But visual person typically can only judge the presence or absence of checking matter according to the depth of color, it is impossible to realize the rational judgment of checking matter.
2. the objectivity of measurement result is poor.Because visual person is subjective to the judgement of shade, easily influenceed by its experience and condition, so ^ is blunt is possible to make different, even wrong judged results in the state of difference to same test strips：Different visually observers can make different, even antipodal judged result because of each different subjectivities to same test strips.
Photoelectric Detection is common technology in basic research and clinical detection, photoelectric detecting technology is with being immunized for example, Chinese utility model patent CN2938080Y discloses a kind of baffled photometer of gold-marking immunity test strips, it is made up of scanning platform, optical system, opto-electronic conversion and signal amplifying system and acquisition and control system, and its optical system is detected by the peak wavelength of multiple illumination paths and a receiving light path group.
Chinese utility model patent CN201373850Y disclose it is a kind of the acetaldehyde detection means that human body kills middle acetaldehyde in a large scale is judged by saliva acetaldehyde test strip, its use two light emitting diodes as transmitting light source.
(the bio-safety gold test strip detector of view-based access control model imaging such as Wang Huimin, spectral instrument and analysis, the 1-3 phases, 170-175 pages) devise it is a kind of the device of the detection of gold label test strip is realized by imaging and image processing techniques, it to the processing of whole reaction solution area image by avoiding due to the error for the testing result that detection light beam is positioned at the diverse location in colour developing area and causes.
But the immune colloid gold test paper optoelectronic detecting device of prior art does not all account for the detection sample due to liquid（Such as serum, urine, saliva）The differences in expansion that diafiltration in immunity colloidal gold test paper strip is caused, this can cause incident light to have different reflections in diverse location, so as to significantly affect the luminous intensity for reaching optical receiver, and then influence final testing result.
Another sunset is foretold, and immune colloid gold test paper photoelectric detecting technology is also commonly used for the detection of a variety of biochemical parameters in clinical sample.For example, the concentration for detecting c reactive protein in blood.
C reactive protein（C-reactive protein, CRP), nineteen thirty Tillet and Francis have found can occur precipitation reaction with pneumococcus C polysaccharide in the presence of calcium ion and gain the name in acute lobar pneumonia patients serum, it is the important acute phase reactive protein of the mankind, acute stage concentration can be raised up to thousands of times.Mankind's c reactive protein is produced by liver, by five identical subunits so that non-covalent bond is connected and forms ring-type pentamer.
C reactive protein can be used as auxiliary judgment foundation currently as hospital's conventional detection project in 4 blunt many medicals diagnosis on disease.C reactive protein starts 6-12 hour concentrations in inflammation and increased, it can reach peak value within 24-48 hours, with the improvement of disease, its concentration can be recovered to normal level rapidly, therefore be commonly used to the prediction of discriminating, the dynamic process of monitoring of diseases, the observation of antibiotic curative effect and accompanying infection of bacterium or virus infection etc..Clinically c reactive protein is detected using common c reactive protein detection kit, test limit is detected typically in 3-5 mg/L or so, nominally reaches the standard grade as 200 mg/L or so, and sensitivity is relatively low.
In recent years, as the incidence of disease of angiocardiopathy is in ascendant trend year by year, high-sensitive C-reactive protein (hsCRP) has been received significant attention as the independent hazard factor of angiocardiopathy.In terms of cardiovascular disease diagnosis, CRP<1 mg/L is low danger, and 1-3 mg/L are poor risk,>3 mg/L are high risk, it is considered to be the important indicator that cardiovascular disease risk is assessed.
The large-scale Biochemical Analyzer volume for being used for c reactive protein detection in Hospitals at Present is larger, it is not readily portable, influenceed seriously by factors such as environment, it is not suitable with on-line checking, detection needs venous blood sampling at present simultaneously and blood sampling volume is big, and centrifugation is needed, detection speed is slow, therefore is not well positioned to meet the requirement of hospital emergency and outpatient service quick diagnosis；Hs-CRP kit detection range is small, it is necessary to being tested after Sample Dilution simultaneously, it is impossible to while suitable for the detection of common c reactive protein.
The content of the invention
It is therefore an object of the present invention to provide a kind of novel biochemical parameter photoelectric detector, sample concentration is quantitatively determined by detecting the colourity change of immune colloid gold strip plate, with portable quick, operation tube list, the characteristics of sensitivity is high.
The instrument is made up of photodetector unit, sensing circuit, control and data processing circuit and data outputting unit, and photodetector unit includes three or more transmitting light sources and an optical receiver or including a transmitting light source and three or more optical receivers, plurality of transmitting light source is arranged about that optical receiver is symmetrical or multiple optical receivers are arranged about transmitting source symmetric distribution.
In an aspect, the present invention relates to a kind of biochemical parameter photoelectric detector, including photodetector unit, sensing circuit, control and data processing circuit and data outputting unit, the photodetector unit includes three or more transmitting light sources and the photoelectric sensor including optical receiver, light from the transmitting light source is used for the detection zone for irradiating strip plate, optical receiver is used to receive come the optical signal for strip plate reflection of hanging oneself, the photodetector unit is arranged to receive the strip plate, and the detection zone of the strip plate aligns with the detection hole in photodetector unit, it is characterized in that to be arranged about optical receiver symmetrical for the transmitting light source, and cause plane where incident ray and strip plate detection zone into 45-75 ° of angle.
In another aspect, the present invention relates to a kind of biochemical parameter photoelectric detector, including photodetector unit, sensing circuit, control and data processing circuit and data outputting unit, the photodetector unit is comprising transmitting light source and includes the photoelectric sensor of three or more optical receivers, light from the transmitting light source is used for the detection zone for irradiating strip plate, optical receiver is used to receive come the optical signal for strip plate reflection of hanging oneself, the photodetector unit is arranged to receive the strip plate, and the detection zone of the strip plate aligns with the detection hole in photodetector unit, it is characterized in that the optical receiver is arranged about transmitting source symmetric distribution, and cause optical receiver to receive the reflection light into 45-75 ° of angle with the plane where strip plate detection zone.
The detection unit can be used as transmitting light source using LED lamps, LED lamps and horizontal line are into 45-75 ° of angle, and 4 LEDs are symmetrically distributed in around photoelectric sensor, the LED is the coloured light that turns white, the wavelength emitted beam is in 380 nm-780 nm, so as to detect the change of colourity using the full paragraph of visible ray.The optical receiving set can use photoelectric receiving tube, primarily serve the effect of position difference compensation, reduce due to the interference that strip Board position difference is brought is immunized.
Or, the detection unit can also be using 1 LED alignment strip plate detection zone, hair
White light, the wavelength emitted beam is in 380-780 nm, and 4 photoelectric receiving tubes and horizontal line are into 45-75.Angle, and 4 photoelectric receiving tubes are symmetrically distributed in around photoelectric sensor, receive the reflected light from strip plate.The effect of position difference compensation is primarily served, is reduced due to the interference that strip Board position difference is brought is immunized.
The output unit includes liquid crystal display and/or embedded printer.
In an embodiment of the photoelectric detector of the present invention, the strip plate is immune colloid gold strip plate.
In an embodiment of the photoelectric detector of the present invention, the biochemical parameter is c reactive protein concentration.
In an embodiment of the photoelectric detector of the present invention, the transmitting light source is 3-8, preferably 4.
In an embodiment of the photoelectric detector of the present invention, the optical receiver is 3-8, preferably 4.
In wherein there is the embodiment of a transmitting light source and multiple optical receiving sets, launch the detection zone of light source vertical incidence strip plate.
In an embodiment of the photoelectric detector of the present invention, the photodetector unit includes extinction cover plate, and it covers the surface around strip plate detection zone receiver hole.
Another object of the present invention, which is to provide, can directly use whole blood or serum as sample, and biochemical parameter photoelectric detector being capable of automatic identification whole blood or serum sample strip plate.
It is a still further object of the present invention to provide the common c reactive protein that a kind of detection speed is fast, detection range is wide and hs-CRP detection method.
Another object of the present invention is to provide a kind of detection method of c reactive protein, and it comprises the following steps：
A. the detection strip plate of multiple c reactive protein standard items is prepared；
B. photosignal value-concentration homologous thread of photosignal value and multiple c reactive protein standard items is set up；
C determines the photosignal value of measuring samples
D. determination c reactive protein concentration is compared by the photosignal value that determines step c and photosignal value-concentration curve for being obtained in b step.
Problem to be solved by this invention is the method detection biochemical parameter quantitatively detected using immune colloid gold and photoelectric sensor, substantially increase detection speed, cylinder operating procedure.In addition, compared with
Wide detection range makes it possible to the diagnosis and prediction in terms of infective inflammation disease and angiocardiopathy.Brief description of the drawings
Fig. 1 is a kind of general structure schematic diagram of embodiment of biochemical parameter photoelectric detector of the present invention.
Fig. 2 is a kind of schematic diagram of embodiment of biochemical parameter photoelectric detector detection unit of the present invention, and which show four light emitting diode/light-sensitive devices and a light-sensitive device/light emitting diode.Embodiment
The biochemical parameter photoelectric detector of the present invention is mainly made up of photodetector unit, sensing circuit, control and data processing circuit and data outputting unit, wherein photodetector unit produces the incident light for irradiating and detecting region and receives the light reflected by detection zone and be converted into electric signal, and it includes light source, optical receiving set and photoelectric conversion device；Sensing circuit sends the electric signal that photodetector unit is produced to control and data processing circuit；Control and data processing circuit are used for the operation and data processing for controlling photoelectric detector；And data outputting unit can show related data and/or detection numeral system can be output into external memory storage or printer.Such as data outputting unit can include liquid crystal display and embedded printer.
Especially, the biochemical parameter photoelectric detector of the present invention includes three or more transmitting light sources and an optical receiver or including a transmitting light source and three or more optical receivers, and plurality of transmitting light source is arranged about that optical receiver is symmetrical or multiple optical receivers are arranged about transmitting source symmetric distribution.
The present invention biochemical parameter photoelectric detector operation and detecting step be conventional.
The Cleaning Principle of the biochemical parameter photoelectric detector of the present invention is collaurum double antibody percolation, when being detected for c reactive protein, and its measurement range is 0 ~ 200 mg/L (normal reference values of c reactive protein<10 mg/L), detection sample can be whole blood, serum or blood plasma, and specimen amount is 10 ul, and the initial measurement time is 1 point of half, one sample of detection in follow-up every 10 seconds, one hour detection limit>250 samples.Explanation.
Fig. 1 is portable biochemical parameter photoelectric detector of the present invention, and as can be seen from the figure it has
Liquid crystal display and external-connected port, strip plate socket is on its top.
Fig. 2 shows a kind of schematic diagram of embodiment of biochemical parameter photoelectric detector detection unit of the present invention, and which show four light emitting diodes as transmitting light source and the light emitting diode as photoelectric sensor.Referring now to the upper figure in Fig. 2, four light emitting diode/light-sensitive devices are equably set around a centrally located light-sensitive device/light emitting diode, C reactive protein strip plate has been inserted into test position to cause detection zone to be directed at centrally located light-sensitive device/light emitting diode, there is a serum whole blood to judge hole on strip plate and also show sleeve pipe, cover plate and the sensitive membrane for colloid gold immune reactive site to show in the type of detection sample, figure.Figure below in Fig. 2 is the sectional view along the A-A lines interception of upper figure, it can be seen that, sleeve pipe can be such that centrally located light-sensitive device/light emitting diode positions exactly, and extinction cover plate covers the surface around strip plate detection zone receiver hole to eliminate the light scattering outside detection zone.Embodiment 1
Experiment purpose：The effect that the checking relatively single led lamp of four LEDs is compensated strip piece position difference.
Experimental program：20 mg/L, 40 mg/L and 100 mg/L, tri- kinds of strip pieces are repeatedly tested, test data is counted, and calculate the coefficient of variation (CV) and are compared.
Strip piece can be prepared according to a variety of methods in this area, be not described in detail herein.Test sample is that 20 mg/L, 40 mg/L and 60 mg/L standard items sample are added dropwise respectively and collaurum is added dropwise to complete the standard strip plate of immune response.
Experimental data：
The raw voltage values that the data of measure obtain for instrument of the invention to the strip plate sampling of three kinds of different colourities.
Table 1 below and 2 be tri- kinds of various concentrations of 20 mg/L, 40 mg/L and 60 mg/L correspondence colourity the strip plate experimental data that the detection under the conditions of 4 LEDs and single LED is obtained for 20 times respectively.
Table 1:
1282 1636 2231
1267 1639 2247
1269 1642 2246
1283 1639 2229
1269 1637 2230
1281 1636 2243
1280 1645 2221
1267 1628 2230
1287 1632 2231
1284 1631 2246
1278 1640 2243
1275 1633 2247
1279 1637 2230
1283 1635 2228
1286 1638 2231
1270 1631 2229
1278 1629 2228
The cv of 1281 1,637 2232 average, 1277.4 1634.95 2234.75 standard deviation 6.68 5.07 8.09 0.52% 0.31% 0.36%
Single LED
The mg/L magnitudes of voltage (mV) 1,229 1498.0 2146 of 20 mg/L, 40 mg/L 100
1259 1515.0 2085
1282 1503.0 2017
1170 1503.0 2014
1175 1529.0 2082
1233 1543.0 2101
1257 1546.0 2199
1192 1568.0 2096
1283 1565.0 2118
1265 1611.0 2007
1306 1617.0 2110
1318 1618.0 2195
1315 1631.0 2175
1324 1619.0 2160
1326 1623.0 2114
1321 1636.0 2121
1317 1626.0 2005
1324 1623.0 2108
1323 1635.0 2129
1329 1634.0 2007
The standard deviation 52.91 51.81 62.11 of average 1277.3 1582.15 2099.45
The experimental results of cv 4.14% 3.27% 2.96%：The comparison of the coefficient of variation (CV) of two groups of measurement data is given in following table.
It can be seen that the relatively single led lamp of four LEDs has the effect of good compensation to strip piece position difference, reduce due to the interference that strip Board position difference is brought is immunized.Embodiment 2. is used for c reactive protein（CRP the Dual channel detection instrument of detection)
The instrument detection of the present invention has low concentration sense channel and high concentration sense channel, and low concentration detection range is 0.5 ~ 18.2 mg/L, and high concentration is more than 18.2 mg/L.Instrument is given tacit consent to and original state detects for low concentration, and when sample liquid concentration is less than 18.2 mg/L, detection blocks in insertion detect tank, instrument
Device measures CRP values less than 18.2 mg/L, directly displays, and completes low concentration detection；If concentration of specimens is more than in 18.2 mg/L, detection card insertion detect tank, instrument measures CRP values more than 18.2 mg/L, and instrument automatically switches to high concentration sense channel, shows CRP values, completes detection.After detection is finished, instrument is automatically restored to acquiescence low concentration detection.
Whole blood sample and the sampled detection of serum sample, the actually detected result of identical is obtained respectively through with whole blood correction curve and the contrast of serum correction curve, but whole blood sample is used directly for detection without being pre-processed.Embodiment 3
Experiment purpose：The effect that four receivers of checking are compensated strip piece position difference.
Experimental program：20 mg/L standard strip piece is repeatedly tested, test data is counted, and calculates CV is compared.
Strip piece can be prepared according to a variety of methods in this area, be not described in detail herein.Test sample is that 20 mg/L standard items sample is added dropwise and collaurum is added dropwise to complete the standard strip plate of immune response.
Experimental data：
The raw voltage values that the data of measure obtain for the instrument of the present invention to the sampling of strip plate.
Table 3 below is that the strip plate of 20 mg/L concentration correspondence colourity detects the experimental data obtained for 20 times under conditions of 4 receivers.
Table 3:
1275 1262 1265 1278 1270
1306 1187 1340 1249 1271
1325 1238 1247 1257 1267
1316 1198 1343 1165 1256
1329 1183 1336 1191 1260
1226 1324 1267 1326 1286
1321 1191 1318 1249 1270
1280 1308 1240 1330 1290
1327 1218 1345 1279 1292
1133 1321 1245 1339 1260
The standard deviation 55.08 52.21 50.82 50.29 17.64 of 1341 1,205 1,325 1,186 1264 average 1,268 1,254 1,271 1,257 1263
CV 4.34% 4.16% 4.00% 4.00% 1.40% can be seen that from upper form：The CV of each single receiver is more than the cv of four receiver average results.
Compared with the CV (4.14%) obtained in embodiment 1 using one LED transmitting light source of single collector correspondence, the CV (1.40%) obtained using 4 receiver correspondences, one transmitting light source is also much lower.
Claims (13)
- Claims1. a kind of biochemical parameter photoelectric detector, including photodetector unit, sensing circuit, control and data processing circuit and data outputting unit, the photodetector unit includes three or more transmitting light sources and the photoelectric sensor including optical receiver, light from the transmitting light source is used for the detection zone for irradiating strip plate, optical receiver is used to receive come the optical signal for strip plate reflection of hanging oneself, the photodetector unit is arranged to receive the strip plate, and the detection zone of the strip plate aligns with the detection hole in photodetector unit, it is characterized in that to be arranged about photelectric receiver symmetrical for the transmitting light source, and cause plane where incident ray and strip plate detection zone into 45-75 ° of angle.2. a kind of biochemical parameter photoelectric detector, including photodetector unit, sensing circuit, control and data processing circuit and data outputting unit, the photodetector unit is comprising transmitting light source and includes the photoelectric sensor of three or more optical receivers, light from the transmitting light source is used for the detection zone for irradiating strip plate, optical receiver is used to receive come the optical signal for strip plate reflection of hanging oneself, the photodetector unit is arranged to receive the strip plate, and the detection zone of the strip plate aligns with the detection hole in photodetector unit, it is characterized in that the optical receiver is arranged about transmitting source symmetric distribution, and cause optical receiver to receive the reflection light into 45-75 ° of angle with the plane where strip plate detection zone.3. photoelectric detector as claimed in claim 1 or 2, wherein the transmitting light source is LED, its wavelength emitted beam is in 380 nm-780 nm.4. such as photoelectric detector of claim 1 or 2, wherein the strip plate is immune colloid gold strip plate.5. photoelectric detector as claimed in claim 4, wherein the biochemical parameter is c reactive protein concentration.6. photoelectric detector as claimed in claim 1, wherein described three or more transmitting light sources are 3-8 light source.7. photoelectric detector as claimed in claim 6, wherein described three or more transmitting light sources are 4 light sources.8. photoelectric detector as claimed in claim 2, wherein described three or more optical receivers are 3-8 optical receiver.9. photoelectric detector as claimed in claim 8, wherein described three or more optical receivers are 4 optical receivers.10. photoelectric detector as claimed in claim 2, wherein the detection zone of transmitting light source vertical incidence strip plate.11. photoelectric detector as claimed in claim 1 or 2, wherein the photodetector unit includes extinction cover plate, it covers the surface around strip plate detection zone receiver hole.12. photoelectric detector as claimed in claim 1 or 2, wherein the photoelectric detector is portable.13. a kind of method that photoelectric detector with as described in claim 1-12 quantitatively detects c reactive protein, its feature comprises the following steps：A, the test section by the c reactive protein immune colloid gold strip plate insertion photodetector unit of load sample.The light that strip plate reflects is converted into electric signal by b, photoelectric sensor, and according to the intensity of electric signal, the concentration of c reactive protein is provided through fitting.
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| PCT/CN2013/075617 WO2014183268A1 (en) | 2013-05-14 | 2013-05-14 | Quantitative detection method of crp using immune colloidal gold test plate and biochemical parameter photoelectric detector |
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| DE102018200162A1 (en) * | 2018-01-08 | 2019-07-11 | Robert Bosch Gmbh | Optical sensor module for spectroscopic measurement |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4568191A (en) * | 1982-07-14 | 1986-02-04 | Compur-Electronic Gmbh | Distance-independent optical reflectance instrument |
| CN2938080Y (en) * | 2006-07-28 | 2007-08-22 | 中国科学院上海光学精密机械研究所 | Reflection photometer of gold-labelled immune test paper |
| CN101346622A (en) * | 2005-07-29 | 2009-01-14 | 必奥康有限公司 | Method and also measurement system for determining the oxygen partial pressure distribution in at least one tissue surface section, in particular skin tissue surface section |
| CN101617213A (en) * | 2007-01-09 | 2009-12-30 | 沃尔夫冈·温霍尔德 | Be used for the method and apparatus that object is checked |
| CN102564954A (en) * | 2010-12-09 | 2012-07-11 | 苏州生物医学工程技术研究所 | Multi-channel photoelectric detection device for dry type chemical analysis |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59114442A (en) * | 1982-12-21 | 1984-07-02 | Mitsui Eng & Shipbuild Co Ltd | Reading sensor of coloration test paper |
| CN2604681Y (en) * | 2003-04-08 | 2004-02-25 | 龙桂忠 | Integrating-sphere probing head |
| CN101514962B (en) * | 2009-03-31 | 2010-11-03 | 东北电力大学 | Fluorescent mark immunity test strip detector |
| CN101887063A (en) * | 2010-07-26 | 2010-11-17 | 沈鹤柏 | Human C-reactive protein colloidal gold immunochromatographic assay quantitative test paper |
-
2013
- 2013-05-14 CN CN201380075557.5A patent/CN105143861A/en active Pending
- 2013-05-14 WO PCT/CN2013/075617 patent/WO2014183268A1/en active Application Filing
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4568191A (en) * | 1982-07-14 | 1986-02-04 | Compur-Electronic Gmbh | Distance-independent optical reflectance instrument |
| CN101346622A (en) * | 2005-07-29 | 2009-01-14 | 必奥康有限公司 | Method and also measurement system for determining the oxygen partial pressure distribution in at least one tissue surface section, in particular skin tissue surface section |
| CN2938080Y (en) * | 2006-07-28 | 2007-08-22 | 中国科学院上海光学精密机械研究所 | Reflection photometer of gold-labelled immune test paper |
| CN101617213A (en) * | 2007-01-09 | 2009-12-30 | 沃尔夫冈·温霍尔德 | Be used for the method and apparatus that object is checked |
| CN102564954A (en) * | 2010-12-09 | 2012-07-11 | 苏州生物医学工程技术研究所 | Multi-channel photoelectric detection device for dry type chemical analysis |
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