CN105123789A - Rhubarb free anthraquinone nanometer emulsion preparation for preventing and treating plant red spider disease - Google Patents
Rhubarb free anthraquinone nanometer emulsion preparation for preventing and treating plant red spider disease Download PDFInfo
- Publication number
- CN105123789A CN105123789A CN201510623246.9A CN201510623246A CN105123789A CN 105123789 A CN105123789 A CN 105123789A CN 201510623246 A CN201510623246 A CN 201510623246A CN 105123789 A CN105123789 A CN 105123789A
- Authority
- CN
- China
- Prior art keywords
- rheum
- percent
- nanometer emulsion
- nano
- rhubarb
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000004056 anthraquinones Chemical class 0.000 title claims abstract description 53
- 241000196324 Embryophyta Species 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 201000010099 disease Diseases 0.000 title claims abstract description 17
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 17
- 239000000839 emulsion Substances 0.000 title abstract description 12
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 title abstract description 11
- 235000009411 Rheum rhabarbarum Nutrition 0.000 title abstract description 10
- 241000488583 Panonychus ulmi Species 0.000 title abstract 4
- 241000219063 Rheum rhabarbarum [NCBITaxon:3621] Species 0.000 title 1
- 241000219061 Rheum Species 0.000 claims abstract description 50
- 239000003814 drug Substances 0.000 claims abstract description 23
- 240000004980 Rheum officinale Species 0.000 claims abstract description 13
- 235000008081 Rheum officinale Nutrition 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000003381 stabilizer Substances 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 235000001018 Hibiscus sabdariffa Nutrition 0.000 claims abstract description 3
- 235000005291 Rumex acetosa Nutrition 0.000 claims abstract description 3
- 240000007001 Rumex acetosella Species 0.000 claims abstract description 3
- 235000003513 sheep sorrel Nutrition 0.000 claims abstract description 3
- 239000007908 nanoemulsion Substances 0.000 claims description 46
- 241001454293 Tetranychus urticae Species 0.000 claims description 25
- 239000000203 mixture Substances 0.000 claims description 7
- GHHURQMJLARIDK-UHFFFAOYSA-N 2-hydroxypropyl octanoate Chemical compound CCCCCCCC(=O)OCC(C)O GHHURQMJLARIDK-UHFFFAOYSA-N 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- LVGKNOAMLMIIKO-UHFFFAOYSA-N Elaidinsaeure-aethylester Natural products CCCCCCCCC=CCCCCCCCC(=O)OCC LVGKNOAMLMIIKO-UHFFFAOYSA-N 0.000 claims description 5
- LVGKNOAMLMIIKO-QXMHVHEDSA-N ethyl oleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC LVGKNOAMLMIIKO-QXMHVHEDSA-N 0.000 claims description 5
- 229940093471 ethyl oleate Drugs 0.000 claims description 5
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims description 5
- 235000010234 sodium benzoate Nutrition 0.000 claims description 5
- 239000004299 sodium benzoate Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 claims description 4
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 claims description 4
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 claims description 4
- 239000005642 Oleic acid Substances 0.000 claims description 4
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 claims description 4
- NTQGILPNLZZOJH-UHFFFAOYSA-N disilicon Chemical compound [Si]#[Si] NTQGILPNLZZOJH-UHFFFAOYSA-N 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 claims description 4
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 claims description 4
- 238000013019 agitation Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000002844 melting Methods 0.000 claims description 3
- 230000008018 melting Effects 0.000 claims description 3
- 239000008213 purified water Substances 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 239000003995 emulsifying agent Substances 0.000 abstract description 21
- 239000003921 oil Substances 0.000 abstract description 10
- 239000003755 preservative agent Substances 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 7
- 239000003795 chemical substances by application Substances 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 4
- 230000000853 biopesticidal effect Effects 0.000 abstract description 2
- 230000002265 prevention Effects 0.000 abstract 2
- 239000007957 coemulsifier Substances 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 11
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 244000061458 Solanum melongena Species 0.000 description 8
- 235000002597 Solanum melongena Nutrition 0.000 description 8
- 239000000853 adhesive Substances 0.000 description 7
- 230000002335 preservative effect Effects 0.000 description 7
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 229940079593 drug Drugs 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- 241000238631 Hexapoda Species 0.000 description 5
- 229920003266 Leaf® Polymers 0.000 description 5
- 239000007921 spray Substances 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 241000721451 Pectinophora gossypiella Species 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 244000000010 microbial pathogen Species 0.000 description 3
- 239000012982 microporous membrane Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- YDQWDHRMZQUTBA-UHFFFAOYSA-N Aloe emodin Chemical compound C1=CC=C2C(=O)C3=CC(CO)=CC(O)=C3C(=O)C2=C1O YDQWDHRMZQUTBA-UHFFFAOYSA-N 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 239000013043 chemical agent Substances 0.000 description 2
- LQGUBLBATBMXHT-UHFFFAOYSA-N chrysophanol Chemical compound C1=CC=C2C(=O)C3=CC(C)=CC(O)=C3C(=O)C2=C1O LQGUBLBATBMXHT-UHFFFAOYSA-N 0.000 description 2
- 239000012043 crude product Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- FFWOKTFYGVYKIR-UHFFFAOYSA-N physcion Chemical compound C1=C(C)C=C2C(=O)C3=CC(OC)=CC(O)=C3C(=O)C2=C1O FFWOKTFYGVYKIR-UHFFFAOYSA-N 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 231100000572 poisoning Toxicity 0.000 description 2
- 230000000607 poisoning effect Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 1
- HIQIXEFWDLTDED-UHFFFAOYSA-N 4-hydroxy-1-piperidin-4-ylpyrrolidin-2-one Chemical group O=C1CC(O)CN1C1CCNCC1 HIQIXEFWDLTDED-UHFFFAOYSA-N 0.000 description 1
- 241000238876 Acari Species 0.000 description 1
- 241000239223 Arachnida Species 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 1
- CTKXFMQHOOWWEB-UHFFFAOYSA-N Ethylene oxide/propylene oxide copolymer Chemical compound CCCOC(C)COCCO CTKXFMQHOOWWEB-UHFFFAOYSA-N 0.000 description 1
- 241000662429 Fenerbahce Species 0.000 description 1
- UGNZSMZSJYOGNX-UHFFFAOYSA-N Isoviocristine Natural products O=C1C=C(C)C(=O)C2=CC3=CC(OC)=CC(O)=C3C(O)=C21 UGNZSMZSJYOGNX-UHFFFAOYSA-N 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 235000008090 Rheum palmatum Nutrition 0.000 description 1
- 240000001745 Rheum palmatum Species 0.000 description 1
- 241000208292 Solanaceae Species 0.000 description 1
- FHNINJWBTRXEBC-UHFFFAOYSA-N Sudan III Chemical compound OC1=CC=C2C=CC=CC2=C1N=NC(C=C1)=CC=C1N=NC1=CC=CC=C1 FHNINJWBTRXEBC-UHFFFAOYSA-N 0.000 description 1
- 206010042566 Superinfection Diseases 0.000 description 1
- 241001454295 Tetranychidae Species 0.000 description 1
- 241000344246 Tetranychus cinnabarinus Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- WLXGUTUUWXVZNM-UHFFFAOYSA-N anthraglycoside A Natural products C1=C(C)C=C2C(=O)C3=CC(OC)=CC(O)=C3C(=O)C2=C1OC1OC(CO)C(O)C(O)C1O WLXGUTUUWXVZNM-UHFFFAOYSA-N 0.000 description 1
- 230000002924 anti-infective effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- NZPQWZZXRKZCDU-UHFFFAOYSA-N chrysophanol Natural products Cc1cc(O)c2C(=O)c3c(O)cccc3Oc2c1 NZPQWZZXRKZCDU-UHFFFAOYSA-N 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- STORWMDPIHOSMF-UHFFFAOYSA-N decanoic acid;octanoic acid;propane-1,2,3-triol Chemical compound OCC(O)CO.CCCCCCCC(O)=O.CCCCCCCCCC(O)=O STORWMDPIHOSMF-UHFFFAOYSA-N 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- UOAMTSKGCBMZTC-UHFFFAOYSA-N dicofol Chemical compound C=1C=C(Cl)C=CC=1C(C(Cl)(Cl)Cl)(O)C1=CC=C(Cl)C=C1 UOAMTSKGCBMZTC-UHFFFAOYSA-N 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- MCWXGJITAZMZEV-UHFFFAOYSA-N dimethoate Chemical compound CNC(=O)CSP(=S)(OC)OC MCWXGJITAZMZEV-UHFFFAOYSA-N 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 229960001617 ethyl hydroxybenzoate Drugs 0.000 description 1
- 235000010228 ethyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004403 ethyl p-hydroxybenzoate Substances 0.000 description 1
- NUVBSKCKDOMJSU-UHFFFAOYSA-N ethylparaben Chemical compound CCOC(=O)C1=CC=C(O)C=C1 NUVBSKCKDOMJSU-UHFFFAOYSA-N 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- NYPJDWWKZLNGGM-UHFFFAOYSA-N fenvalerate Chemical compound C=1C=C(Cl)C=CC=1C(C(C)C)C(=O)OC(C#N)C(C=1)=CC=CC=1OC1=CC=CC=C1 NYPJDWWKZLNGGM-UHFFFAOYSA-N 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- XUGNVMKQXJXZCD-UHFFFAOYSA-N isopropyl palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC(C)C XUGNVMKQXJXZCD-UHFFFAOYSA-N 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 229960003511 macrogol Drugs 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
- 239000004530 micro-emulsion Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000000324 neuroprotective effect Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- PKUBGLYEOAJPEG-UHFFFAOYSA-N physcion Natural products C1=C(C)C=C2C(=O)C3=CC(C)=CC(O)=C3C(=O)C2=C1O PKUBGLYEOAJPEG-UHFFFAOYSA-N 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 229920001983 poloxamer Polymers 0.000 description 1
- 229920001993 poloxamer 188 Polymers 0.000 description 1
- 229940044519 poloxamer 188 Drugs 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- ZYHMJXZULPZUED-UHFFFAOYSA-N propargite Chemical compound C1=CC(C(C)(C)C)=CC=C1OC1C(OS(=O)OCC#C)CCCC1 ZYHMJXZULPZUED-UHFFFAOYSA-N 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 238000000967 suction filtration Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Landscapes
- Medicinal Preparation (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of biopesticide, and particularly relates to a recipe and a preparation process of a rhubarb free anthraquinone nanometer emulsion preparation, and application of the rhubarb free anthraquinone nanometer emulsion preparation to the prevention and the treatment of the plant red spider disease. The rhubarb free anthraquinone is extracted from traditional Chinese medicine sorrel, rheum tanguticum or rheum officinale. Through being metered by the mass percentage of each ingredient contained in the nanometer emulsion, the content of the rhubarb free anthraquinone in the nanometer emulsion accounts for 0.05 to 0.16 percent; the oil phase in the nanometer emulsion accounts for 18 to 22 percent; the emulsifiers account for 16 to 20 percent; co-emulsifiers account for 4 to 6 percent; water accounts for 50 to 61 percent; stabilizing agents account for 1.0 percent; preservatives account for 0.1 percent; attaching agents account for 0.1 percent. The rhubarb free anthraquinone nanometer emulsion preparation can be used for preventing and treating the plant red spider disease. The free anthraquinone from the rhubarb free anthraquinone nanometer emulsion preparation disclosed by the invention is from the traditional chinese medicine rhubarb; the applicability of the extraction process is high; the raw material obtaining cost is low; the nanometer emulsion preparation is automatically formed; the process is simple; the quality is controllable; the mass production can be easily realized; excellent prevention and treatment effects are achieved on the plant red spider disease.
Description
Technical field
The invention belongs to pesticide field, particularly relating to a kind of free anthraquinones extracted from rheum nano-emulsion preparation for preventing and treating plant two spotted spider mite disease.
Background technology
Plant two spotted spider mite disease is extensively present on the plant stem-leafs such as various crop, vegetables, flowers, trees, the crops such as main harm Solanaceae, Curcurbitaceae, pulse family, Liliaceae and grass family.The two spotted spider mite kind of China, based on Tetranychus cinnabarinus, belongs to arachnids, Acarina, Tetranychidae, and distributed areas are extensive, and feeding habits are mixed, and can endanger 110 various plants.Two spotted spider mite breeding amount is large, and polypide is little not easily to be found, once find that it endanger, transfer velocity is fast, insect pest area greatly, and repeatedly superinfection crops.Hazard approach thrusts in blade with mouthpart to suck juice, and chlorophyll is damaged, and blade presents sallow point or patch, until whole blade orange, come off, the serious normal growth of plant that hinders is grown, and causes huge economic loss.At present the main agricultural chemicals used has the control plant two spotted spider mite such as propargite, dicofol, Rogor, pink bollworm are clean, Belmark sick.But along with the long-time use of chemical agent, two spotted spider mite is to the resistance which created in various degree, and control efficiency significantly reduces, and day by day the manifesting of the drawback such as chemical agent environmental pollution and high residue.Therefore, develop toxicity little, pollute little, residual less, the biopesticide of drug resistance can be avoided to be the task of top priority.
Botanical fungicide has person poultry safety, environmental friendliness, can natural degradation and the advantages such as noresidue.Rheum officinale [comprises sorrel (Rheumpalmatum); secondary to Rheum tanguticum Maxim (Rheumpalmatumvar.tanguticum) and Rheum officinale (Rheumofficinale)] be one of conventional plant resource Chinese medicine; its extract have rush down under, the effect such as anti-infective and neuroprotective activity, but have no the research of its inhibitory or killing effect to plant two spotted spider mite.Pharmaceutical dosage form can produce significant impact to the control efficiency of medicine, but kills activity as wetting powder and supensoid agent present by means of only the contacting of pathogenic microorganism on solid drug particles and plant corpus surface, but kills weak effect to the pathogenic microorganism of inside plants; Missible oil is not good in the adhesiveness of plant surface, ductility, farthest can not play the effect of medicine; The particle diameter of microemulsion usually at micron order, but effectively can not kill the pathogenic microorganism of inside plants tissue through the epidermis of plant stem-leaf.Nano-emulsion is less because playing particle diameter, has good mobility, can more effectively infiltrate in plant base of leaf, plays the biologically active of medicine, and preparation technology simple, can mix with arbitrary proportion with water, do not block shower nozzle, easy to use.Up to now, do not find that the sick active technology of the anti-plant two spotted spider mite of free anthraquinones extracted from rheum nano-emulsion preparation is open.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, there is provided a kind of using traditional Chinese medicine rheum officinale as raw material, the free anthraquinones extracted from rheum that obtained by acidolysis, abstraction and purification (mainly containing archen, aloe-emodin, Chrysophanol, Physcion and Rhein) is bulk drug, research and develop a kind of be exclusively used in control plant two spotted spider mite disease nano-emulsion preparation.
Technical scheme of the present invention: a kind of free anthraquinones extracted from rheum nano-emulsion preparation for preventing and treating plant two spotted spider mite disease, make with following raw material: with the mass percent of each component contained in nano-emulsion, free anthraquinones extracted from rheum content is 0.05-0.16%, and the oil phase in nano-emulsion accounts for 18 ~ 22%, emulsifier 16 ~ 20%, assistant for emulsifying agent 4 ~ 6%, water 50 ~ 61%, stabilizing agent 1.0%, preservative 0.1%, adhesive agent 0.1%.
Described oil phase is selected from isopropyl myristate, Sefsol 218, ethyl oleate, isopropyl palmitate, Miglyol 812N, castor oil and combination thereof.Wherein, preferred further Sefsol 218 and the mixture with ethyl oleate thereof, oil phase concentration is less than 30%.Described emulsifier is selected from polyoxyethylene-40-rilanit special, Labraso, Gelucire 44/14, Tween-80, poloxamer-188 and combination thereof.Wherein, preferred further polyoxyethylene-40-rilanit special; Described assistant for emulsifying agent is selected from TC, Macrogol 600, PEG400,1,2-PD, glycerine and combination thereof.Wherein preferred further TC, the amount of assistant for emulsifying agent is less than 20%; The mass ratio of emulsifier and assistant for emulsifying agent is 2:1.Described water is solvent, and stabilizing agent is oleic acid or enuatrol or sodium citrate, preferred oleic acid; Preservative is Sodium Benzoate and ethylparaben, preferred sodium benzoate; Adhesive agent is disilicon trioxide.
A kind of free anthraquinones extracted from rheum nano-emulsion preparation for preventing and treating plant two spotted spider mite disease of the present invention, its preparation method is: be dissolved in by free anthraquinones extracted from rheum in oil phase, add stabilizing agent, and emulsifier/assistant for emulsifying agent mixture, stirs.Dropwise add water, stir, self-emulsifying forms the nano-emulsion preparation of clear, after ultrasonic process 15-30min, with 1mol/LNaOH regulating system pH to 6.0-7.0, then adds preservative and adhesive agent in proportion.Emulsifier need 35-40 DEG C of heating and melting before using, magnetic agitation speed 200-300rpm.Directly use during the packing of gained nano-emulsion preparation in the water system filtering with microporous membrane in 0.22 μm of aperture to sterilized brown plastic containers.
Nanoemulsion particle diameter of the present invention between 20-100nm, ζ-potential between-20 ~-50mV, viscosity between 8-12cP, pH between 6.0-7.0.
Free anthraquinones extracted from rheum nano-emulsion of the present invention has the purposes of control plant two spotted spider mite disease, and excellent effect.
The invention has the beneficial effects as follows:
The raw materials used medicine of first product of the present invention is traditional Chinese medicine free anthraquinones extracted from rheum extract, nontoxicly within the scope of using dosage to have no drug resistance, safety is high, Financial cost is lower;
It two is that product of the present invention can be rendered as the homogeneous system of transparent and stable with the mixing of the water of arbitrary proportion, and preparation technology is simple, is easy to produce in enormous quantities, and emulsion droplet size is little, and viscosity is little, and field is sprayed and do not blocked sprayer, easy to use;
It three is that the two spotted spider mite disease of product of the present invention to the crops such as cucumber, eggplant, corn and flowers has significant control efficiency, and its effect is equivalent or to be better than flowers pink bollworm clean.
Accompanying drawing explanation
Fig. 1 is the free anthraquinones extracted from rheum nano-emulsion transmission electron microscope photo (× 15000) of the embodiment of the present invention 2
Fig. 2 is the sick photo of control plant two spotted spider mite of embodiment 4.
Embodiment
Below in conjunction with embodiment, the present invention will be further described, but be not limited thereto.Wherein emulsifier polyoxyethylene-40-rilanit special is on sale in BASF Chemical Co., Ltd.; Sefsol 218, TC, Gelucire 44/14, Labraso are on sale in French Gattefosse company; PLURONICS F87 is on sale in Adamas company of Switzerland.Other each raw material components all can be buied in market.
Embodiment 1: the extraction of free anthraquinones extracted from rheum
Experiment condition
Rheum officinale, purchased from Xin Sheng source, Li County biotechnology development corporation, Ltd., gets the Rhubarb pulverized and sieved, and add 20% sulfuric acid heating acidolysis, the dregs of a decoction are washed to neutral post-drying.Bulk drug pressed by rheum officinale hydrolysis dry powder: 90% ethanol=1:30 (w/v) mixes, ultrasonic extraction, suction filtration while hot, filtrate reduced in volume to without alcohol taste, with chloroform extraction, it is colourless that chloroform extraction liquid is extracted to upper liquid with 5%NaOH again, merge alkali extract, adjust pH about to 2, centrifuging and taking precipitates, and be washed to neutrality, obtain free anthraquinones extracted from rheum crude product.After crude product drying, by bulk drug: methyl alcohol=1:10 adds methyl alcohol ultrasonic dissolution, the decompression of centrifuging and taking supernatant is spin-dried for, and obtains free anthraquinones extracted from rheum (wherein five kinds of dissociated anthraquinones account for more than 98% of gross mass).
Embodiment 2: the prescription of free anthraquinones extracted from rheum nano-emulsion and preparation
Prescription one:
Free anthraquinones extracted from rheum 0.130g, oil phase 19.5g, emulsifier 16.5g, assistant for emulsifying agent 4.5g, stabilizing agent 1.0g, preservative 0.1g, adhesive agent 0.1g, purified water 58.4g, gross weight is about 100.0g.
Prescription two:
Free anthraquinones extracted from rheum 0.098g, oil phase 18.4g, emulsifier 16.0g, assistant for emulsifying agent 4.0g, stabilizing agent 1.0g, preservative 0.1g, adhesive agent 0.1g, purified water 60.5g, gross weight is about 100.0g.
In prescription: free anthraquinones extracted from rheum is main ingredient, oil phase is the mixture (prescription one) of Sefsol 218 (prescription two) or itself and ethyl oleate, emulsifier is polyoxyethylene-40-rilanit special, assistant for emulsifying agent is TC, stabilizing agent is oleic acid, preservative is Sodium Benzoate, and adhesive agent is disilicon trioxide.
Preparation method is:
Free anthraquinones extracted from rheum and stabilizing agent are dissolved in oil phase, add the mixture of emulsifier/assistant for emulsifying agent, stir.Dropwise add water, at the uniform velocity stir, self-emulsifying forms the nano-emulsion of clear, and regulating system pH6.0-7.0, then add preservative and adhesive agent.Emulsifier is at room temperature solid, therefore needs 35-40 DEG C of heating and melting before use.During the packing of gained nano-emulsion preparation, directly with in the water system filtering with microporous membrane in 0.22 μm of aperture to sterilized brown plastic containers.
Embodiment 3: the type of free anthraquinones extracted from rheum nano-emulsion, character, assay and stability
Experimental condition: the nano-emulsion that embodiment 2 prescription one is obtained, test as follows:
Nano-emulsion type identification: adopt decoration method to differentiate nano-emulsion type, the speed utilizing oil-soluble dyes tonyred and water-soluble dye methylene blue to spread in nano-emulsion is to judge the type of nano-emulsion.
Nano-emulsion character: mode of appearance, light transmittance, pH value, viscosity, particle diameter and Zeta potential.
Assay: get free anthraquinones extracted from rheum nano-emulsion 1ml, add 10ml methyl alcohol, ultrasonic emulsion breaking, the filtering with microporous membrane of 0.45 μm, high performance liquid chromatography instrument (HPLC) is analyzed, and calculates total free anthraquinone content in free anthraquinones extracted from rheum nano-emulsion.
Study on the stability: (1) influence factor is tested: by under nano-emulsion respectively high light, high temperature and hot conditions, in sampling in the 5th day, the 10th day, whether the character of observing free anthraquinones extracted from rheum nano-emulsion changed.(2) centrifugal acceleration test: after centrifugal for nano-emulsion 12000rpm 30min, observes whether have layering or precipitation.(2) normal temperature keeps sample test: free anthraquinones extracted from rheum nano-emulsion is placed 90 days at normal temperatures, respectively at sampling in the 30th day, 60 days, 90 days, investigates nano-emulsion character and medicament contg.Result of the test:
Free anthraquinones extracted from rheum nano-emulsion type is O/W type, and under transmission electron microscope, observe emulsion droplet is spheroidal, and light transmittance is 99.0-99.9%, pH is 6.4-6.7, and viscosity is 10.0-11.5cP, and particle diameter is between 20-100nm, and ζ-potential is-25.0 ~-35.0mV.
In free anthraquinones extracted from rheum nano-emulsion, free anthraquinone content is 0.122% (w/w).Nano-emulsion preparation measure each component of free anthraquinones extracted from rheum between ratio and extract in each component ratio scope substantially close, its error mainly derives from the same treatment of different batches rheum officinale, and this is acceptable for traditional Chinese medicine material extract.
Stability test shows that free anthraquinones extracted from rheum nano-emulsion is placed under these conditions, and form is clear still, and the phenomenon such as layering, flocculation does not occur.In centrifugal acceleration test, after the centrifugal 30min of nano-emulsion 12000rpm, still clear is homogeneous.The normal temperature test that keeps sample shows that nano-emulsion room temperature places 90 days, and physical stability is good, and the content of rhubarb free general anthraquinone and each monomer remains unchanged substantially.
The test of embodiment 4 free anthraquinones extracted from rheum nano-emulsion anti-plant two spotted spider mite disease
Major target disease: eggplant two spotted spider mite is sick
Test field: * * * vegetable field
Test grouping: blank group need not any reagent (being labeled as E respectively in following table); Drug component three dilution groups: be respectively free anthraquinones extracted from rheum nano-emulsion 100 times, 250 times and 500 times of dilutions (being labeled as A, B, C respectively in following table); Positive controls is 800 times of dilutions (being labeled as D respectively in following table) of the clean every 10g of flowers pink bollworm.
Testing program: eggplant (cool eggplant No. 2, Wuwei Institute of agricultural sciences seed selection), the two spotted spider mite onste phase, often processes 3 repetitions, often repeats to spray 5m
2, often repeat to select 2 strain eggplants at random before spray medicine, select 2 intact leafs of two spotted spider mite generation moderate quatity before spray medicine at every strain different parts, hang label and look into several movable mite number.2nd, 5 day investigative action mite number after spray medicine, calculates Revision insect recluced rate and control efficiency.Compare with blank group, calculate Revision insect recluced rate and control efficiency.
Borer population alive before Revision insect recluced rate rate %=[borer population of living after borer population-dispenser of living before dispenser] × 100/ dispenser
Control efficiency %=[treatment region decline rate-check plot decline rate] × 100/ [100-check plot Revision insect recluced rate]
Result of the test (table 1):
Table 1 different agents is to the controlling experiment of eggplant two spotted spider mite disease
As can be seen from the test results, in medicament of participating in the experiment used, free anthraquinones extracted from rheum nano-emulsion 100 times of dilutions are best to two spotted spider mite control efficiency, and effective drug duration is long, strong to the control of two spotted spider mite, are a kind of favourable agents preventing and treating two spotted spider mite; Free anthraquinones extracted from rheum nano-emulsion 250 times is suitable with pink bollworm clean 800 times of dilution process control efficiency of 2 to 5 days, all more than 85%, all can promote the use of aborning.
Find in preventive effect fact-finding process, although the control efficiency of free anthraquinones extracted from rheum nano-emulsion 100 times of dilutions to eggplant two spotted spider mite disease is fabulous, it accelerates the withered of eggplant leaf, demonstrates certain poisoning.And obvious poisoning does not appear in free anthraquinones extracted from rheum nano-emulsion 250 times of dilutions, can use safely.Optimum control period about 5 days, optionally row sprays medicine more afterwards.
Above content is in conjunction with concrete preferred embodiment further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, under the premise of not departing from the present invention, some simple deduction or replace can also be made, all should be considered as belonging to protection scope of the present invention.
Claims (4)
1. a free anthraquinones extracted from rheum composition, is characterized in that, described free anthraquinones extracted from rheum composition stems from the extract of traditional Chinese medicine sorrel, Rheum tanguticum Maxim or Rheum officinale.
2. a free anthraquinones extracted from rheum nano-emulsion preparation, it is made up of following raw material:
Free anthraquinones extracted from rheum 0.05 ~ 0.16%, Sefsol 218 13 ~ 16%, ethyl oleate 4 ~ 6%, polyoxyethylene-40-rilanit special 16 ~ 20%, TC 4 ~ 6%, oleic acid 1.0%, Sodium Benzoate 0.1%, disilicon trioxide 0.1%, purified water 50 ~ 61%.
3. a kind of free anthraquinones extracted from rheum nano-emulsion preparation as claimed in claim 2, prepare as follows: free anthraquinones extracted from rheum and stabilizing agent are dissolved in Sefsol 218 and ethyl oleate, add polyoxyethylene-40-rilanit special, TC, stir, dropwise add water, Keep agitation, until the liquid forming clear, 1mol/LNaOH regulating system pH6.0-7.0 after ultrasonic process 15-30min, then add Sodium Benzoate and disilicon trioxide.Polyoxyethylene-40-rilanit special needs 35-40 DEG C of heating and melting, magnetic agitation speed 200-300rpm before using.
4. a kind of free anthraquinones extracted from rheum nano-emulsion as described in claim 1-3 has the purposes of control plant two spotted spider mite disease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510623246.9A CN105123789B (en) | 2015-09-25 | 2015-09-25 | A kind of free anthraquinones extracted from rheum nano-emulsion preparation for being used to prevent and treat plant red spider disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510623246.9A CN105123789B (en) | 2015-09-25 | 2015-09-25 | A kind of free anthraquinones extracted from rheum nano-emulsion preparation for being used to prevent and treat plant red spider disease |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105123789A true CN105123789A (en) | 2015-12-09 |
| CN105123789B CN105123789B (en) | 2017-10-20 |
Family
ID=54709679
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510623246.9A Active CN105123789B (en) | 2015-09-25 | 2015-09-25 | A kind of free anthraquinones extracted from rheum nano-emulsion preparation for being used to prevent and treat plant red spider disease |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105123789B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110448534A (en) * | 2019-07-29 | 2019-11-15 | 兰州大学 | A kind of free anthraquinones extracted from rheum self-emulsifying microemulsion piece and preparation method thereof |
| CN113244302A (en) * | 2021-02-22 | 2021-08-13 | 兰州大学 | Rhubarb free anthraquinone composition for regulating blood fat |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4602004A (en) * | 1984-06-14 | 1986-07-22 | Maurice Cohen | Aloin and aloe-emodin containing pesticides |
| CN1345597A (en) * | 2000-09-26 | 2002-04-24 | 上海市中药研究所 | Extract of free anthraquinone extracted from rhubarb and preparation process thereof |
| CN101347516A (en) * | 2008-08-01 | 2009-01-21 | 石任兵 | Method for preparing total free anthraquinones extract from rhubarb |
| CN102595885A (en) * | 2009-10-05 | 2012-07-18 | 玛珑生物创新有限公司 | Anthroquinone containing derivatives as biochemical agricultural products |
| CN103330754A (en) * | 2013-06-19 | 2013-10-02 | 陇东学院 | Method for extracting total anthraquinone from fresh rheum officinale |
| CN104586961A (en) * | 2014-12-29 | 2015-05-06 | 南通市第三人民医院 | Method for extracting total anthraquinones in rhubarb |
| CN104642312A (en) * | 2015-02-13 | 2015-05-27 | 兰州大学 | Rheum officinale free anthraquinone composition for preventing plant powdery mildew and nano emulsion preparation |
-
2015
- 2015-09-25 CN CN201510623246.9A patent/CN105123789B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4602004A (en) * | 1984-06-14 | 1986-07-22 | Maurice Cohen | Aloin and aloe-emodin containing pesticides |
| CN1345597A (en) * | 2000-09-26 | 2002-04-24 | 上海市中药研究所 | Extract of free anthraquinone extracted from rhubarb and preparation process thereof |
| CN101347516A (en) * | 2008-08-01 | 2009-01-21 | 石任兵 | Method for preparing total free anthraquinones extract from rhubarb |
| CN102595885A (en) * | 2009-10-05 | 2012-07-18 | 玛珑生物创新有限公司 | Anthroquinone containing derivatives as biochemical agricultural products |
| CN103330754A (en) * | 2013-06-19 | 2013-10-02 | 陇东学院 | Method for extracting total anthraquinone from fresh rheum officinale |
| CN104586961A (en) * | 2014-12-29 | 2015-05-06 | 南通市第三人民医院 | Method for extracting total anthraquinones in rhubarb |
| CN104642312A (en) * | 2015-02-13 | 2015-05-27 | 兰州大学 | Rheum officinale free anthraquinone composition for preventing plant powdery mildew and nano emulsion preparation |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110448534A (en) * | 2019-07-29 | 2019-11-15 | 兰州大学 | A kind of free anthraquinones extracted from rheum self-emulsifying microemulsion piece and preparation method thereof |
| CN113244302A (en) * | 2021-02-22 | 2021-08-13 | 兰州大学 | Rhubarb free anthraquinone composition for regulating blood fat |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105123789B (en) | 2017-10-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104642312A (en) | Rheum officinale free anthraquinone composition for preventing plant powdery mildew and nano emulsion preparation | |
| CN103875722B (en) | Chinese herbal medicine merges the botanical fungicide extracted | |
| CN105248489B (en) | A kind of biological agent for tea place preventing and treating pest and disease damage and preparation method thereof | |
| CN102754667A (en) | High-efficiency composite botanical pesticide | |
| CN103814964B (en) | A kind of emulsion for killing aphid and two spotted spider mite and preparation method thereof | |
| CN110476963A (en) | A kind of tank-mix adjuvants and its preparation method and application | |
| CN102239890B (en) | Microcapsule suspension used for preventing and controlling spiraling whitefly and preparation method thereof | |
| CN104186495B (en) | A kind of SYP-Zo48-Osthole suspended emulsion and preparation method thereof | |
| CN106332921B (en) | A kind of plant insecticide and its preparation method and application | |
| CN105123789A (en) | Rhubarb free anthraquinone nanometer emulsion preparation for preventing and treating plant red spider disease | |
| CN106489930A (en) | A kind of exhibition film oil-suspending agent containing Bacillus thuringiensis and its application in preventing and treating crop pests | |
| CN103271103B (en) | Botanical complex insecticide containing matrine and aconitum flavum hand alkaloid and preparation method of botanical complex insecticide | |
| CN107873755A (en) | Vegetable source pesticide, bactericidal microemulsion and preparation method thereof and application method | |
| CN104542690A (en) | Lambda-cyhalothrin emulsifiable granule and preparation method and application thereof | |
| CN110973169A (en) | Biological pesticide of raspberry leaf alcohol extract and preparation method thereof | |
| CN103734204B (en) | Pesticide composite containing schizonepeta and tobacco extract, preparation method and application | |
| CN106172557A (en) | A kind of Folium Camelliae sinensis common pests herbal control agent and preparation method thereof | |
| CN1647644A (en) | Natural pyrethrin cream preparation | |
| CN105851097A (en) | Botanical insecticide for preventing and controlling cotton bollworm and preparing method thereof | |
| CN106172401B (en) | It is a kind of to eliminate propargite on citrus using the propargite preparation for generating phytotoxicity | |
| CN106259515A (en) | A kind of mango insecticide and preparation method thereof | |
| CN108260601A (en) | A kind of jasmin long-acting mosquito-driving liquid and preparation method thereof | |
| CN104604896A (en) | Composition capable of preventing and eliminating cuscuta parasitic plant, preparation and applications thereof | |
| CN105660686B (en) | A kind of leek maggot attractant and preparation method and application | |
| Umbreen et al. | Sacred Basil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231221 Address after: 742200 Shibei Village, Chengguan Town, Lixian County, Longnan City, Gansu Province Patentee after: SHENG XIN SOURCE BIOLOGICAL TECHNICAL DEVELOPING CO.,LTD. Address before: 730000 Lanzhou University, 222 Tianshui South Road, Chengguan District, Lanzhou City, Gansu Province Patentee before: LANZHOU University |
|
| TR01 | Transfer of patent right |