CN105115782B - Manual crop leaf sample collector - Google Patents

Manual crop leaf sample collector Download PDF

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Publication number
CN105115782B
CN105115782B CN201510487095.9A CN201510487095A CN105115782B CN 105115782 B CN105115782 B CN 105115782B CN 201510487095 A CN201510487095 A CN 201510487095A CN 105115782 B CN105115782 B CN 105115782B
Authority
CN
China
Prior art keywords
handle
blade
knife rest
installation portion
positioning sleeve
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201510487095.9A
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Chinese (zh)
Other versions
CN105115782A (en
Inventor
张全芳
步迅
张荦嘉
范阳阳
汝医
郭庆法
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biotechnology Research Center of Shandong Academy of Agricultural Sciences
Original Assignee
Biotechnology Research Center of Shandong Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Biotechnology Research Center of Shandong Academy of Agricultural Sciences filed Critical Biotechnology Research Center of Shandong Academy of Agricultural Sciences
Priority to CN201510487095.9A priority Critical patent/CN105115782B/en
Publication of CN105115782A publication Critical patent/CN105115782A/en
Application granted granted Critical
Publication of CN105115782B publication Critical patent/CN105115782B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

Manual crop leaf sample collector, easily blade is polluted during for solving the problems, such as low blade sampling efficiency, more sub-samplings.It includes be hinged first handle and second handle, the free end of first handle is the first installation portion, hollow positioning sleeve is removably installed on the first installation portion, the first knife rest is removably installed in the free end of positioning sleeve, the first blade is fixed with the first knife rest;Movable post is provided with the inner chamber of positioning sleeve, the second knife rest is removably installed on movable post, the second blade is fixed on the second knife rest;The free end of second handle is the second installation portion, and punching press seat and probe tube are provided with the second installation portion.Pen clip is provided with first or second handle, marking pen is installed on pen clip, during operation, it is only necessary to which the personal hand-held sampler, operation is flexibly, conveniently;And first knife rest and the dismountable connected mode of the second knife rest, it is easy to clean the first blade and the second blade.

Description

Manual crop leaf sample collector
Technical field
The present invention relates to crop DNA detection technique fields, specifically a kind of manual crop leaf sample collector.
Background technology
When carrying out DNA detections to crop, first have to be sampled crop, the method for sampling there are two kinds of sides of punching and shearing Method, the blade laid or sheared is collected into container, and container is numbered.Prior art is during blade punches Two people are needed to complete, a personal hand-held blade, another person's hand-held hole punch and sampling pipe, this sample mode waste labor The efficiency that power and blade sample is low.The prior art of another blade sampling can allow adopting for personal progress blade Sample, but card punch blade is non-exchange, and easily blade is polluted when sampling again, influences the accuracy of experimental data; Sampling pipe clamp only adapts to the sampling pipe of single model;It is also inconvenient to the probe tube label after the completion of sampling.
The content of the invention
It is an object of the invention to provide one kind to make analyte detection leaf harvest device manually, for solving blade sampling efficiency The problem of easily being polluted during low, more sub-samplings to blade.
The technical scheme adopted by the invention to solve the technical problem is that:Manual crop leaf sample collector, including hinge The first handle and second handle connect in succession, it is characterized in that, torsion spring is provided between the first handle and second handle, is being turned round The drive end of first handle and the drive end of second handle are located remotely from each other in the presence of spring;The free end of the first handle is the One installation portion, hollow positioning sleeve is removably installed on first installation portion, can in the free end of the positioning sleeve Dismounting is provided with the first knife rest, and the first blade of circle is fixed with first knife rest;In the inner chamber of the positioning sleeve In be fixed with nut, on the nut coordinate installation activity post, can on the movable post in positioning sleeve free end Dismounting is provided with the second knife rest, and the second blade of circle is fixed with second knife rest;The freedom of the second handle Hold as the second installation portion, through hole is provided with second installation portion, punching press seat, the punching press are provided with the top of the through hole Seat includes the external mold that is detachably connected and internal model, and the external mold and internal model are hollow structure, and the interior chamber size of the internal model Identical with the external diameter of the second blade, the interior chamber size of the external mold is identical with the external diameter of the first blade;In the lower section of the through hole Dismountable to be provided with probe tube, the internal diameter of the probe tube is more than the external diameter of the first blade;
Pen clip is provided with the first handle or second handle, marking pen is placed on the pen clip.
Further, first knife rest and the second knife rest are annular, and first knife rest and positioning sleeve are free End threaded connection, second knife rest are connected with movable male screw.
Further, the external mold is threadedly coupled with the second installation portion, and the internal model is threadedly coupled with external mold.
Further, two fixing clips and a movable clip block are provided with the bottom surface of second installation portion, it is described Fixing clip is identical with movable clip block planform, and the fixing clip and movable clip block are uniformly distributed on same circumference;Institute State movable clip block to be slidably connected with the second installation portion, and spring is provided between movable clip block and the second installation portion, described solid It is respectively equipped with neck on clamp block and movable clip block, the neck synergy clamping probe tube in fixing clip and movable clip block.
Further, slide rail is provided with second installation portion, the movable clip block is slidably connected with slide rail.
Further, anti-skid design is provided with the movable clip block.
The beneficial effects of the invention are as follows:Manual crop leaf sample collector provided by the invention, sets on first handle Staking punch is put, punching press seat is set on second handle, the blade that staking punch is pointed between the two with punching press seat mating reaction enters Row punching press, to obtain a certain size blade sample.Pass through the cooperation of the cooperation of the first blade and external mold, the second blade and internal model Different size of blade sample can be obtained.During operation, it is only necessary to which the personal hand-held sampler, operation is flexibly, conveniently;And the One knife rest and the dismountable connected mode of the second knife rest, are easy to clean the first blade and the second blade.Pen clip and mark The setting of pen, the operation that a people can be facilitated to be sampled and mark, to avoid obscuring for probe tube.
Brief description of the drawings
Fig. 1 is the schematic diagram of the present invention;
Fig. 2 is the A-A sectional views in Fig. 1;
Fig. 3 is the scheme of installation of the first blade;
Fig. 4 is the schematic diagram of punching press seat;
Fig. 5 is B in Fig. 1 to local direction view;
Fig. 6 is partial enlarged drawing at the C in Fig. 5;
In figure:1 first handle, 11 first installation portions, 2 second handles, 21 second installation portions, 3 is be hinged, 4 positioning sleeves, and 41 certainly By holding, 42 nuts, 43 movable posts, 5 first knife rests, 51 first blades, 6 second knife rests, 61 second blades, 7 external molds, in 71 first Chamber, 72 internal models, 73 second inner chambers, 8 sampling bottles, 81 edges, 9 fixing clips, 91 movable clip blocks, 92 slide rails, 93 springs, 10 Folder.
Embodiment
As shown in Figures 1 to 6, the invention mainly comprises first handle 1, second handle 2, positioning sleeve 4, the first knife rest 5, One blade 51, the second knife rest 6, the second blade 61, punching press seat and sampling bottle fixation assembly, the present invention is carried out below in conjunction with the accompanying drawings It is described in detail.
As shown in figure 1, first handle 1 and second handle 2 are hinged 3 connections by rotating shaft, torsion spring is provided with rotating shaft, is being turned round The drive end of first handle and the drive end of second handle are mutually drawn close in the presence of spring.The free end of first handle is for pacifying The first installation portion 11 of staking punch is filled, the free end of second handle is the second installation portion 21 for installing punching press seat.
As shown in Fig. 2 being provided with screwed hole on the first installation portion, coordinate in screwed hole and positioning sleeve 4, positioning sleeve are installed For the hollow plastic article of circle, the outer wall of positioning sleeve installation end is provided with external screw thread, and the free end 41 of positioning sleeve is pacified through first Side extension where backward second installation portion in dress portion, screw thread is provided with the first knife rest 5 on free end, as shown in figure 3, first Knife rest is the working of plastics or metalwork of annular, and internal thread is provided with the first knife rest inwall, is fixed in one end of the first knife rest First blade 51 of annular is installed.Nut 42 is installed with the inner chamber of positioning sleeve, internal thread is provided with nut, Coordinate on nut and movable post 43 is installed, movable post is connected with nut thread.The internal diameter of positioning sleeve installation end is more than free end Internal diameter, the external diameter of positioning sleeve installation end is more than the external diameter of free end, and the external diameter of movable post is less than the internal diameter of free end, movable post One end be located at outside positioning sleeve, the other end of movable post is located in positioning sleeve free end, is being placed in the work of positioning sleeve free end Screw thread is provided with the second knife rest 6 of annular on dynamic post, and the second blade 61 of annular is installed with the second knife rest.The One knife rest is threadedly coupled with positioning sleeve free end, the second knife rest is connected with movable male screw, is easy to the dismounting of first, second knife rest And installation.The external diameter of second blade is less than the external diameter of the first blade, rotary moveable post, may be such that the second blade is placed in the first blade Above or below.
As shown in figure 4, being provided with through hole on the second installation portion, external mold 7 is installed in the upper square thread of through hole, external mold is circle The metalwork or working of plastics of annular, the first inner chamber 71 on external mold are used to carry out punching press to blade with the effect of the first blades fit, The internal diameter of first inner chamber is equal to the external diameter of the first blade.Internal model 72 is installed in the upper square thread of external mold, internal model is integral into annulus Shape, annular groove is provided with the bottom of internal model, the internal thread being threadedly coupled with external mold is provided with the inwall of annular groove, during internal model is Hollow structure, the second inner chamber 73 of internal model are used for and punching press of the second blades fit effect realization to blade, the internal diameter of second inner chamber Equal to the external diameter of the second blade.
As shown in figure 5, being provided with two fixing clips 9 and a movable clip block 91 in the bottom of the second installation portion, two solid Clamp block and a movable clip block are located on same circumference, and two fixing clips and the planform of a movable clip block are homogeneous Together, three is uniformly distributed on same circumference, i.e., the angle between two adjacent fixing clips, fixing clip and movable clip block is equal For 120 degree.The neck of arc is respectively equipped with fixing clip and movable clip block, fixing clip and movable clip block are used to clamp admittedly Determine probe tube 8.As shown in fig. 6, being provided with slide rail 92 on the bottom surface of the second installation portion, movable clip block is slidably connected with slide rail, Spring 91 is provided between slide rail and movable clip block, under the action of the spring, movable clip block and the distance between fixing clip are most short. When placing probe tube, the edge 81 of probe tube is placed in the neck of two fixing clips first, then slides in and out movable folder Block so that then the nose end of probe tube unclamps movable clip block parallel to the bottom surface of the second installation portion, under the action of the spring, Movable clip block and fixing clip clamp probe tube.Now, hand-holdable first handle and second handle, sample collection is carried out.For Increasing friction force, anti-skid design is provided with movable clip block, in order to which operator's hand promotes movable clip block.
The leaf sample collector, sets staking punch on first handle, and punching press seat, staking punch are set on second handle The blade being pointed between the two with punching press seat mating reaction carries out punching press, to obtain a certain size blade sample.Pass through The cooperation of the cooperation of one blade and external mold, the second blade and internal model can obtain different size of blade sample.During operation, only need The personal hand-held sampler is wanted, staking punch and punching press seat are respectively placed in the both sides of blade, presses first, second handle, can So that staking punch carries out punching press to blade with the mating reaction of punching press seat, operation is flexibly, conveniently.During to avoid gathering different leaves DNA pollution, when having gathered the sample collection of the different plants of blade sample progress, the first knife rest can be unloaded from positioning sleeve Or unload the second knife rest from movable post, change clean the first knife rest and the second knife rest.After the completion of sampling, in fact Now to the numbering of probe tube, pen clip 10 is provided with first handle, marking pen is placed on pen clip, after probe tube is taken down, led to Marking pen is crossed to be numbered on probe tube.When being sampled to kindred plant blade, then it need not change, clean blade.

Claims (6)

1. manual crop leaf sample collector, including the first handle and second handle being articulated and connected, it is characterized in that, described Torsion spring is provided between first handle and second handle, the driving of the drive end and second handle of first handle in the presence of torsion spring End is located remotely from each other;The free end of the first handle is the first installation portion, is removably provided with first installation portion Hollow positioning sleeve, the first knife rest is removably installed in the free end of the positioning sleeve, it is fixed on first knife rest There is the first blade of circle;Nut is fixed with the inner chamber of the positioning sleeve, installation activity post is coordinated on the nut, The second knife rest is removably installed on the movable post in positioning sleeve free end, is fixed with second knife rest The second circular blade;The free end of the second handle is the second installation portion, and through hole is provided with second installation portion, The top of the through hole is provided with punching press seat, and the punching press seat includes external mold and internal model, the external mold and the internal model being detachably connected It is hollow structure, and the interior chamber size of the internal model is identical with the external diameter of the second blade, the interior chamber size of the external mold and the The external diameter of one blade is identical;Probe tube is removably installed in the lower section of the through hole, the internal diameter of the probe tube is more than the The external diameter of one blade;
Pen clip is provided with the first handle or second handle, marking pen is placed on the pen clip.
2. manual crop leaf sample collector according to claim 1, it is characterized in that, first knife rest and the second knife Frame is annular, and first knife rest is threadedly coupled with positioning sleeve free end, and second knife rest connects with movable male screw Connect.
3. manual crop leaf sample collector according to claim 1, it is characterized in that, the external mold and the second installation portion Threaded connection, the internal model are threadedly coupled with external mold.
4. manual crop leaf sample collector according to claim 3, it is characterized in that, at the bottom of second installation portion Face is provided with two fixing clips and a movable clip block, and the fixing clip is identical with movable clip block planform, described solid Clamp block and movable clip block are uniformly distributed on same circumference;The movable clip block is slidably connected with the second installation portion, and in work It is dynamic that spring is provided between fixture block and the second installation portion, neck, geometrical clamp are respectively equipped with the fixing clip and movable clip block Neck synergy clamping probe tube on block and movable clip block.
5. manual crop leaf sample collector according to claim 4, it is characterized in that, set on second installation portion There is slide rail, the movable clip block is slidably connected with slide rail.
6. manual crop leaf sample collector according to claim 5, it is characterized in that, it is provided with the movable clip block Anti-skid design.
CN201510487095.9A 2015-08-10 2015-08-10 Manual crop leaf sample collector Expired - Fee Related CN105115782B (en)

Priority Applications (1)

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CN201510487095.9A CN105115782B (en) 2015-08-10 2015-08-10 Manual crop leaf sample collector

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
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CN105115782B true CN105115782B (en) 2018-01-09

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105598888A (en) * 2016-03-30 2016-05-25 中国矿业大学 Clamp capable of protecting testing tube
CN113959751B (en) * 2021-09-22 2022-04-01 中国农业科学院蔬菜花卉研究所 Instrument for collecting plant samples

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1811280A1 (en) * 2006-01-18 2007-07-25 Seiko Instruments R&D Center Inc. Automatic sliced piece fabricating apparatus and automatic sliced piece sample fabricating apparatus
CN202162853U (en) * 2011-07-25 2012-03-14 山东省农作物种质资源中心 Multi-functional blade puncher
CN202846180U (en) * 2012-07-28 2013-04-03 广东联塑科技实业有限公司 Fixture of tube cutting machine
CN103439141A (en) * 2013-08-27 2013-12-11 中国农业科学院棉花研究所 Functional leaf sampler
CN203579776U (en) * 2013-12-05 2014-05-07 任雯 Blade hole puncher
CN104359084A (en) * 2014-10-08 2015-02-18 安徽世林照明股份有限公司 Backlight module fluorescent tube bracket
CN204359554U (en) * 2015-01-01 2015-05-27 中国烟草总公司郑州烟草研究院 A kind of hand-held card punch being applicable to tobacco leaf in situ sampling and collecting
CN204495592U (en) * 2015-03-24 2015-07-22 罗赛男 Portable leaf dish sampler
CN204903213U (en) * 2015-08-10 2015-12-23 山东省农业科学院生物技术研究中心 Manual crop blade sample collection ware

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008050525A1 (en) * 2008-10-06 2010-04-22 Leica Biosystems Nussloch Gmbh Device and method for identifying at least one slide

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1811280A1 (en) * 2006-01-18 2007-07-25 Seiko Instruments R&D Center Inc. Automatic sliced piece fabricating apparatus and automatic sliced piece sample fabricating apparatus
CN202162853U (en) * 2011-07-25 2012-03-14 山东省农作物种质资源中心 Multi-functional blade puncher
CN202846180U (en) * 2012-07-28 2013-04-03 广东联塑科技实业有限公司 Fixture of tube cutting machine
CN103439141A (en) * 2013-08-27 2013-12-11 中国农业科学院棉花研究所 Functional leaf sampler
CN203579776U (en) * 2013-12-05 2014-05-07 任雯 Blade hole puncher
CN104359084A (en) * 2014-10-08 2015-02-18 安徽世林照明股份有限公司 Backlight module fluorescent tube bracket
CN204359554U (en) * 2015-01-01 2015-05-27 中国烟草总公司郑州烟草研究院 A kind of hand-held card punch being applicable to tobacco leaf in situ sampling and collecting
CN204495592U (en) * 2015-03-24 2015-07-22 罗赛男 Portable leaf dish sampler
CN204903213U (en) * 2015-08-10 2015-12-23 山东省农业科学院生物技术研究中心 Manual crop blade sample collection ware

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
从叶片上获取外植体的改良方法——打孑L器法;徐伟君;《农技服务》;20091231;第145页 *
叶面积测定法;庄建守;《新疆农业科学》;19791231(第3期);第23-24页 *

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CB03 Change of inventor or designer information

Inventor after: Zhang Quanfang

Inventor after: Bu Xun

Inventor after: Zhang Luojia

Inventor after: Fan Yangyang

Inventor after: Ru Yi

Inventor after: Guo Qingfa

Inventor before: Zhang Quanfang

Inventor before: Bu Xun

Inventor before: Fan Yangyang

Inventor before: Ru Yi

Inventor before: Guo Qingfa

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CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20180109

Termination date: 20190810