CN105053509A - Red heart dragon fruit seed albumin polypeptide, preparation and application thereof - Google Patents
Red heart dragon fruit seed albumin polypeptide, preparation and application thereof Download PDFInfo
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- CN105053509A CN105053509A CN201510548705.1A CN201510548705A CN105053509A CN 105053509 A CN105053509 A CN 105053509A CN 201510548705 A CN201510548705 A CN 201510548705A CN 105053509 A CN105053509 A CN 105053509A
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- dragon fruit
- red heart
- heart dragon
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Abstract
The present invention discloses a red heart dragon fruit seed albumin polypeptide, a preparation and an application thereof. The technical scheme relates to a red heart dragon fruit seed albumin polypeptide which has a sequence of KQKAQILPNVCNLQSRRCEIGTITTVK. The preparation method comprises steps that: (1) taking red heart dragon fruit seeds out from the fruit pulp, air-drying the seeds, and crushing the seed with a crusher; (2) adding 100 g of red heart dragon fruit seed power into 100-500 ml of water, carrying out water bath at a temperature of 40-80 DEG C for 60-120 minutes; (3) performing centrifuging at 2000-3000 rpm for 10-30 minutes, and taking supernatant; (4) adding the supernatant into a buffer with a pH value of 4-5, and carrying out stirring; (5) performing centrifuging at 10000-15000 rpm for 10-30 minutes, and collecting precipitate; (6) reconstituting the precipitate into a buffer with a pH value of 7-9, and separating out a single ingredient by a gel chromatography column; and (7) performing freeze-drying to obtain the product. In vitro experiments showed that the polypeptide can form complexes with arsenic, lead and other heavy metals, so that pesticide residues such as arsenic and lead are less absorbed by human body.
Description
Technical field
The present invention relates to red heart dragon fruit seed peptide art, be specifically related to red heart dragon fruit seed albumin polypeptide preparation field.
Background technology
Dragon fruit contains the rare vegetable albumin of general plant, nutritious, function is unique, is a kind of health-care nutritional food consumed notional green, environmental protection fruit and have certain curative effect.It is reported, the nutrition of dragon fruit seed is particularly abundant, is mainly rich in phytalbumin.Because industrial wastewater, offal treatment are reclaimed and do not obtained attention, so that various water resource receives serious heavy metal pollution, and except direct drinking water is poisoning, the mankind take the photograph people's food content of beary metal and exceed standard also easily poisoning.And albumin when running into heavy metal ion in human body, automatically can be combined with heavy metal ion, be got rid of by excretory system external, play the effect of removing toxic substances.
The nutritive value of red heart dragon fruit is far away higher than common dragon fruit.Present stage, from red heart dragon fruit seed, do not extract albuminous report.Also not about the report of albumin polypeptide for detoxifying in red heart dragon fruit seed.
Summary of the invention:
Goal of the invention:
The object of this invention is to provide a kind of albumin polypeptide extracted from red heart dragon fruit seed, and preparation method and application.
Technical scheme
Technical scheme of the present invention is red heart dragon fruit seed albumin polypeptide, and its sequence is KQKAQILPNVCNLQSRRCEIGTITTVK.Polypeptide preparation step comprises (1) and is taken out from pulp by red heart dragon fruit seed, after drying, pulverizes with pulverizer; (2) red heart dragon fruit seed powder 100g adds in 100-500ml water, water-bath 40-80 DEG C, 60-120 minute; (3) centrifugal 2000-3000rpm, 10-30 minute, get supernatant; (4) add in the buffer solution of pH4-5, stir; (5) centrifugal 10000-15000rpm, 10-30 minute, collecting precipitation thing; (6) sediment is redissolved in the buffer solution of pH7-9, adopt gel chromatography chromatographic column to isolate single component; (7) freeze-drying, to obtain final product.Its red heart dragon fruit seed albumin polypeptide can be used for removing residues of pesticides, described medicament residue heavy metal arsenic, lead.
Useful benefit:
The red heart dragon fruit seed albumin polypeptide prepared of the present invention, through protein sequencing, sequence is KQKAQILPNVCNLQSRRCEIGTITTVK, and purity is greater than 70%.Experiment in vitro shows, this polypeptide can form complex compound with the heavy metal such as arsenic, lead, thus reduces residues of pesticides, as heavy metal arsenic, plumbous absorption in vivo.
Detailed description of the invention
Embodiment 1
Red heart dragon fruit seed is taken out from pulp, after drying, pulverizes with pulverizer; Red heart dragon fruit seed powder 100g is added in 100ml water, water-bath 40 DEG C, 120 minutes; Centrifugal 2000rpm, 10 minutes, gets supernatant; In supernatant, add in the buffer solution of pH4.5, stir; Centrifugal 10000rpm, 30 minutes, collecting precipitation thing; Redissolved by sediment in the buffer solution of pH7, adopt gel chromatography chromatographic column (SephadexG25) to isolate single component, mobile phase is PBS, pH7.2; Freeze-drying, to obtain final product.The sequence adopting solid phase protein automatic sequencer to measure this polypeptide is KQKAQILPNVCNLQSRRCEIGTITTVK, and purity is 86.4%.
Embodiment 2
Red heart dragon fruit seed is taken out from pulp, after drying, pulverizes with pulverizer; Red heart dragon fruit seed powder 100g is added in 500ml water, water-bath 80 DEG C, 120 minutes; Centrifugal 3000rpm, 15 minutes, gets supernatant; In supernatant, add in the buffer solution of pH5, stir; Centrifugal 15000rpm, 60 minutes, collecting precipitation thing; Redissolved by sediment in the buffer solution of pH9, adopt gel chromatography chromatographic column (SephadexG25) to isolate single component, mobile phase is PBS, pH7.2; Freeze-drying, to obtain final product.The sequence adopting solid phase protein automatic sequencer to measure this polypeptide is KQKAQILPNVCNLQSRRCEIGTITTVK, and purity is 76.3%.
Embodiment 3
Measure the effect that the polypeptide that obtains of embodiment 1 reduces plumbum ion concentration in lead solution: the lead nitrate solution 2ml getting 1mg/mL, altogether an experiment 4 pipe, add respectively pure water, 0.5,1, the polypeptide of 2mg/ml, after stirring, leave standstill 15 minutes, centrifugal 15000rpm, after 10 minutes, get supernatant solution.The atomic absorption spectrography (AAS) of lead ion is adopted to measure.Result shows, water, 0.5,1, plumbous concentration is respectively in the polypeptide of 2mg/ml: 991,0.062,0.046,0.025 μ g/L.This experiment repetition 3 times.As can be seen here, the polypeptide prepared of the present invention conspicuousness can reduce the content of lead ion in lead solution.
Embodiment 4
The polypeptide that mensuration embodiment 1 obtains reduces the effect of arsenic ion concentration in lead solution: the accurate arsenic solution of label taking (1ug/ml) 2ml, an experiment 4 pipe altogether, add respectively pure 0.5,1, the polypeptide of 2mg/ml, after stirring, leave standstill 15 minutes, centrifugal 15000rpm, after 10 minutes, gets supernatant solution.Arsenic spot method is adopted to carry out the mensuration of arsenic content.Result shows, water, 0.5,1, plumbous concentration is respectively in the polypeptide of 2mg/ml: 0.99,0.047,0.026,0.015 μ g/ml.This experiment repetition 3 times.As can be seen here, the polypeptide prepared of the present invention conspicuousness can reduce the content of pentavalent arsenic ion in arsenic solution.
SEQUENCELISTING
<110> Biotechnology Co., Ltd. Changzhou Adam
<120> a red heart dragon fruit seed albumin polypeptide, preparations and applicatio
<130>
<160>1
<170>PatentInversion3.3
<210>1
<211>27
<212>PRT
<213> artificial sequence
<400>1
LysGlnLysAlaGlnIleLeuProAsnValCysAsnLeuGlnSerArg
151015
ArgCysGluIleGlyThrIleThrThrValLys
2025
Claims (3)
1. a red heart dragon fruit seed albumin polypeptide, is characterized in that: sequence is KQKAQILPNVCNLQSRRCEIGTITTVK.
2. the red heart dragon fruit seed albumin polypeptide preparation according to claims 1: it is characterized in that: step comprises, and red heart dragon fruit seed takes out by (1) from pulp, after drying, pulverize with pulverizer; (2) red heart dragon fruit seed powder 100g adds in 100-500ml water, water-bath 40-80 DEG C, 60-120 minute; (3) centrifugal 2000-3000rpm, 10-30 minute, get supernatant; (4) add in the buffer solution of pH4-5, stir; (5) centrifugal 10000-15000rpm, 10-30 minute, collecting precipitation thing; (6) sediment is redissolved in the buffer solution of pH7-9, adopt gel chromatography chromatographic column to isolate single component; (7) freeze-drying, to obtain final product.
3. the red heart dragon fruit seed albumin polypeptide according to claims 1 can be used for removing residues of pesticides, and described medicament residue is heavy metal arsenic, lead.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510548705.1A CN105053509A (en) | 2015-08-31 | 2015-08-31 | Red heart dragon fruit seed albumin polypeptide, preparation and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510548705.1A CN105053509A (en) | 2015-08-31 | 2015-08-31 | Red heart dragon fruit seed albumin polypeptide, preparation and application thereof |
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| CN105053509A true CN105053509A (en) | 2015-11-18 |
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| CN201510548705.1A Pending CN105053509A (en) | 2015-08-31 | 2015-08-31 | Red heart dragon fruit seed albumin polypeptide, preparation and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106720915A (en) * | 2016-12-07 | 2017-05-31 | 常州亚当生物技术有限公司 | The processing method of dragon fruit |
| CN109156690A (en) * | 2018-09-23 | 2019-01-08 | 李红光 | A kind of dragon fruit toxin-expelling and face nourishing electuary |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103288967A (en) * | 2013-05-20 | 2013-09-11 | 广州柏纵生物科技有限公司 | Establishment and expression of fusion protein having function of adsorbing heavy metal ions, and application fusion protein in bioremediation |
| CN104186919A (en) * | 2014-08-26 | 2014-12-10 | 常州亚当生物技术有限公司 | Dragon fruit-derived polypeptide as well as application and preparation method thereof |
-
2015
- 2015-08-31 CN CN201510548705.1A patent/CN105053509A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103288967A (en) * | 2013-05-20 | 2013-09-11 | 广州柏纵生物科技有限公司 | Establishment and expression of fusion protein having function of adsorbing heavy metal ions, and application fusion protein in bioremediation |
| CN104186919A (en) * | 2014-08-26 | 2014-12-10 | 常州亚当生物技术有限公司 | Dragon fruit-derived polypeptide as well as application and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 张纯丽等: "活性多肽提取分离方法研究进展", 《农产品加工（学刊）》 * |
| 张美莉等: "植物蛋白源生物活性肽的研究进展", 《中国食物与营养》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106720915A (en) * | 2016-12-07 | 2017-05-31 | 常州亚当生物技术有限公司 | The processing method of dragon fruit |
| CN109156690A (en) * | 2018-09-23 | 2019-01-08 | 李红光 | A kind of dragon fruit toxin-expelling and face nourishing electuary |
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Application publication date: 20151118 |