CN105010193B - One kind hybridizes Fugu seed pond breeding method then - Google Patents

One kind hybridizes Fugu seed pond breeding method then Download PDF

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CN105010193B
CN105010193B CN201510448015.9A CN201510448015A CN105010193B CN 105010193 B CN105010193 B CN 105010193B CN 201510448015 A CN201510448015 A CN 201510448015A CN 105010193 B CN105010193 B CN 105010193B
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pond
seed
water
fugu
days
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CN105010193A (en
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张年国
潘桂平
周文玉
周裕华
刘本伟
侯文杰
毕浩
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Shanghai Fisheries Research Institute
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Shanghai Fisheries Research Institute
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

Abstract

One kind hybridizes Fugu seed pond breeding method then, it is characterized in that seed sterilizes before putting in a suitable place to breed to pond desilting, splashed compound fertilizer culture biological feed after the water inlet of pond, seed body length need to be strong up to more than 8mm, energy, put before seedling by seedling pond water salinity gradually salinization to pond culture salinity, seed moves band water operation, the ㎡ of the tail of seed breeding density 4500/1000;Seed delivers not bait throwing at initial stage, when biological feed is not enough in pond, by soya-bean milk of splashing, freeze big artemia, reduce seed to cut one another's throat, to improve weed survival rate, seed body feeds into bulk eel mixed bait after being up to 20mm, cultivate early stage, change water once within 15 days, cultivate the middle and later periods, change water frequency and be adjusted to 7 days once;Biological agent of being splashed in breeding process to pond prevents the generation of parasite;When pond water temperature is near less than 18 DEG C, hybridization fish is transferred to heat preservation greenhouse and survived the winter, fishing style uses trawlnet, trawlnet uses knotless net, it is to avoid to fish body skin abrasion.

Description

One kind hybridizes Fugu seed pond breeding method then
Technical field
The present invention relates to technical field of aquaculture, especially hybridize Fugu seed pond breeding method.
Background technology
Fugu obscurus (Takifugu obscurus) and takifugu flavidus (Takifugu flavidus) are under the jurisdiction of Tetraodontiformes (Tetraodontif-ormes), Molidae (Tetraodontidae), Fugu category (Fugu).Fugu obscurus is near Hai Nuanwen water borne base migration fishes, the East China Sea and the Yellow Sea, middle and lower reach of Yangtze River region are distributed mainly in China, and spring parent population is traced back River migration is bred into fresh water rivers tributary, and juvenile fish is fattened in fresh water;Takifugu flavidus is commonly called as ship bar, babysbreath, main The coastal immediate offshore area of northwest Pacific is distributed in, the marine sites such as the East China Sea and the Yellow Sea and the Bohai Sea are distributed mainly in China, category temperate zone is near Subsea level fish.Recently as the increasingly mature of cultural technique, the cultivation scale of fugu obscurus and takifugu flavidus also exists Constantly expand, both self-characteristics determine that it cultivates future, and fugu obscurus fast growth, 1-2 just can reach Listing requirement, but its economic value is low compared with takifugu flavidus, although and takifugu flavidus value is higher, but the dark line of its speed of growth Fugu is slow, commercial specification is can be only achieved after general 3 years, therefore develop a kind of fast growth, market acceptance height, cultivation valency The big new varieties of value are just particularly important.
According to hybrid organisms characteristic, if fugu obscurus and takifugu flavidus are hybridized, reconfigure gene, It is oriented to cultivate, it is expected to obtain the hybridization for possessing parent both sides' merit or producing the unexistent new merit of parent Kind, that is, cultivate the river Puffer cultivation new varieties that fast growth, individual are big, meat is good and have extensive market acceptance.At present It is less about the research report in terms of Fugu hybridization, it is concentrated mainly on takifugu flavidus, Fugu rubripes, yellowfin Fugu etc. Kind, and hybridize F about fugu obscurus raun and takifugu flavidus milter1For pond rearing method then there is not yet open report Road.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of hybridization Fugu seed pond breeding method then, solve Fugu obscurus raun produces with takifugu flavidus milter hybrid seedlings large-scale cultivation, improves breeding survival rate and be easy to pipe Reason, the large-scale promotion for hybridization Fugu provides technical support.
Technical scheme is selected by pond, fertilising of cleaning up the pond, seed are delivered, pond management, disease control and fished for Step is constituted, it is characterized in that pond selection is in the place having a good transport service, water quality is good, pond requires environment quiet, had abundant water resources Pollution-free, length-width ratio is 2:1, water level is maintained at more than 150cm, enters draining conveniently, discharge outlet sets anti-escape filter screen;Put in seed Need to sterilize pond desilting before supporting, sterilization quick lime, consumption is 90-150kg/1000 ㎡, changes full pool spilling head after water, pond is entered Water is used after 80 mesh sieve tulles bag filtrations, the 100cm that adds water, and compound fertilizer of being splashed into pond carries out rich water culture underlying biological bait Material, consumption is 7.5kg/1000 ㎡, is carried out imposing composite fertilizer according to water body color, it is in dark brown or yellow green to make water body;Deliver Seed body length need to reach that more than 8mm, energy are strong, put before seedling must by seedling pool inner water body salinity gradually salinization to pond culture salt Degree, seed needs band water operation during moving, and seed breeding density is the ㎡ of 4500 tails/1000;Pond management is delivered in seed Substantially not bait throwing in 1-6 days initial stage, by the underlying biological bait in pond waters, if underlying biological bait is not enough in pond, By soya-bean milk of splashing, big artemia is freezed, seed is reduced and cuts one another's throat, to improve the survival rate of hybridization Fugu seed, treat seed Body gradually feeds the eel mixed bait for being processed into bulk after being up to 20mm, and twice, bait morning feeding volume is accounted for entirely for daily bait throwing in It 60%, account for 40% afternoon, summer feeding volume accounts for that fish body weight ratio is few compared with spring and autumn, and daily feeding volume is entered at any time by material feeding situation Row adjustment, to be eaten up in after bait throwing in 2 hours as appropriate, the change of observation pond water quality and material feeding situation in daily management, in seed In 45 days after putting in a suitable place to breed, water was changed once every 15 days, each quantity of exchanged water is until before fishing for, changing water frequency from 30%, 46 days It is adjusted to 7 days once, every time 50%;Disease control be splashed in whole breeding process into cultivating pool every 15 days it is commercially available Substrate modifier (efficient substrate is clear, white+black matrix changes king) and EM bacteria microorganism preparations, the parasitic argulus of prevention fish body, in progress It is preferential to use EM bacteria microorganism preparations during medical treatment;When pond water temperature drop is to less than 18 DEG C, just hybridization fish is transferred to Heat preservation greenhouse is survived the winter, and fishing style uses trawl type, and trawlnet uses knotless net, it is to avoid to fish body skin abrasion.
The fugu obscurus raun of the invention hybridizes F with takifugu flavidus milter1Implementing for pond rearing method In achieve remarkable result, utilize outdoor ponds ecological method carry out fugu obscurus (♀) × takifugu flavidus (♂) cross hybrid seedling The large-scale production planted is cultivated, and makes its survival rate to more than 90%, during pond culture, 1 age hybridization Fugu individual Greatly, grow fast, premunition strong, body weight be his father, maternal 1.5 times, with obvious hybrid vigour, be expected to cultivate hybridization east Square Puffer new lines.
Embodiment
In order that the technological means that the present invention is implemented is easy to understand, it is expanded on further, has with reference to instantiation Body example comprises the following steps:
1st, pond selection is in the place having a good transport service, water quality is good, pond require environment quiet, have abundant water resources it is pollution-free, Length-width ratio is 2:1, water level is maintained at more than 150cm, enters draining conveniently, discharge outlet sets anti-escape filter screen;Shanghai City aquatic products research Institute Qidong scientific base has a good transport service, water quality is good, environment quiet, have abundant water resources it is pollution-free, with long 80m, wide 40m, north and south Cultivating pool is moved towards, cultivation water level is maintained at more than 150cm, enters draining conveniently, discharge outlet sets anti-escape filter screen, meets culturing pool Primary condition is selected on the pool.
2nd, clean up the pond fertilising:Need to sterilize pond desilting before seed is put in a suitable place to breed, sterilization quick lime, consumption is 90-150kg/ 1000 ㎡, change full pool spilling head after water;Pond water inlet is used just splashes again after 80 mesh sieve tulles bag filtrations, the 100cm that adds water into pond Hefei material (N-P-K=15-15-15) carries out the basic biological feed of rich water culture, and consumption is 7.5kg/1000 ㎡, can be according to water body Color carries out imposing compound fertilizer, water body is kept dark brown, yellow green preferably.
3rd, seed is delivered:Seed reaches more than 8mm and seedling pool inner water body salinity is gradually after salinization to the identical salinity in pond, just Can be delivered in cultivating pool cultivate, seed needed during moving band water operation, seed breeding density be 4500 tails/ 1000㎡。
4th, pond is managed:Not bait throwing in substantially in 1-6 days initial stage is delivered in seed, by the underlying biological bait in pond waters Material, if underlying biological bait is not enough in pond, by soya-bean milk of splashing, freezes big artemia, reduces seed and cut one another's throat, to improve Hybridize the survival rate of Fugu seed, the eel mixed bait for being processed into bulk is gradually fed after seed body is up to 20mm, often Twice, bait morning feeding volume accounts for whole day 60% for its bait throwing in, accounts for 40% afternoon, summer feeding volume accounts for fish body weight ratio compared with spring and autumn Few, daily feeding volume is adjusted at any time by material feeding situation, to be eaten up in after bait throwing in 2 hours as appropriate, observation in daily management Pond water quality changes and material feeding situation, in 45 days after seed is put in a suitable place to breed, water is changed once every 15 days, each quantity of exchanged water is Until before fishing for, changing water frequency and being adjusted to 7 days once from 30%, 46 days, every time 50%.
5th, disease control:In whole breeding process, commercially available substrate modifier of being splashed every 15 days into cultivating pool (efficient substrate is clear, white+black matrix changes king) and EM bacteria microorganism preparations, the parasitic argulus of prevention fish body are excellent when carrying out medical treatment First use EM bacteria microorganism preparations.
6th, when pond water temperature drop is to less than 18 DEG C, hybridization fish is just transferred to heat preservation greenhouse and survived the winter, fishing style is adopted With trawl type, trawlnet uses knotless net, it is to avoid to fish body skin abrasion.
The object of hybrid seedlings Juvenile stage, fugu obscurus (Takifugu obscurus) is used as maternal, chrysanthemum Huang east Puffer (Takifugu flavidus) is used as male parent.1 age hybridization Fugu and the growing state of its Parent offspring are contrasted during harvest It is shown in Table 1.
The control group 1 of table 1:Using the hybridization same female parent of Fugu;Control group 2:Using the hybridization same male parent of Fugu.
As shown in Table 1, hybridization Fugu has obvious growth vigor, compared with control group, a length of control group 1 of body 1.17 times, 1.29 times of control group 2, total length is 1.20 times, 1.26 times of control group 2 of control group 1, and body weight is control group 1 1.67 times, 1.76 times of control group 2.It can thus be seen that the hybridization Fugu kind that this case method is obtained incorporates father and mother The multinomial merit of this parent population, shows obvious heterosis, hybrid vigor, with good market development prospect.

Claims (2)

1. a kind of pond rearing method for hybridizing Fugu seed then, selected by pond, fertilising of cleaning up the pond, seed dispensing, pond Management, disease control and step composition is fished for, it is characterized in that pond selection is in the place having a good transport service, water quality is good, pond will Seek environment quiet, have abundant water resources pollution-free, length-width ratio is 2:1, water level is maintained at more than 150cm, enters draining conveniently, discharge outlet is set Put anti-escape filter screen;Need to sterilize pond desilting before seed is put in a suitable place to breed, sterilization quick lime, consumption is 90-150kg/1000 ㎡, is changed Full pool spilling head after water, pond water inlet is used after 80 mesh sieve tulles bag filtrations, the 100cm that adds water, and compound fertilizer of being splashed into pond is carried out Rich water culture basis biological feed, consumption is 7.5kg/1000 ㎡, is carried out imposing composite fertilizer according to water body color, it is in tea to make water body Brown or yellow green;The seed body length of dispensing need to reach that more than 8mm, energy are strong, put before seedling must by seedling pool inner water body salinity by Gradually salinization is to pond culture salinity, and seed needs band water operation during moving, and seed breeding density is the ㎡ of 4500 tails/1000; Pond management delivers not bait throwing in substantially in 1-6 days initial stage in seed, by the underlying biological bait in pond waters, if base in pond When plinth biological feed is not enough, by soya-bean milk of splashing, big artemia is freezed, seed is reduced and cuts one another's throat, to improve hybridization Fugu seedling Kind survival rate, gradually fed after seed body is up to 20mm and be processed into the eel mixed bait of bulk, daily bait throwing in twice, bait Material morning feeding volume accounts for whole day 60%, accounts for 40% afternoon, and it is few compared with spring and autumn that summer feeding volume accounts for hybridization Fugu body weight ratio, often Its feeding volume is adjusted at any time by material feeding situation, and to be eaten up in after bait throwing in 2 hours to be appropriate, pond water is observed in daily management Qualitative change and material feeding situation, water was changed once in 45 days after seed is put in a suitable place to breed every 15 days, each quantity of exchanged water be 30%, 46 days from Until before fishing for, change water frequency and be adjusted to 7 days once, every time 50%;Disease control is every 15 in whole breeding process Its substrate modifier for being splashed into cultivating pool commercially available and EM bacteria microorganism preparations, prevention hybridization Fugu fish body parasitic fish Lice, when carrying out medical treatment, uses EM bacteria microorganism preparations;When pond water temperature drop is to less than 18 DEG C, just hybridization east Square Puffer is transferred to heat preservation greenhouse and survived the winter, and fishing style uses trawl type, and trawlnet uses knotless net, it is to avoid to hybridization Fugu fish body Skin abrasion.
2. a kind of pond rearing method for hybridizing Fugu seed then according to claim 1, it is characterised in that described Hybridize the object of Fugu seed Juvenile stage then, fugu obscurus is used as male parent as female parent, takifugu flavidus.
CN201510448015.9A 2015-07-27 2015-07-27 One kind hybridizes Fugu seed pond breeding method then Active CN105010193B (en)

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CN107079849A (en) * 2017-06-09 2017-08-22 扬中市水产技术研究所 A kind of method that fugu obscurus fry Tu Chi is cultivated
CN109601438A (en) * 2019-01-28 2019-04-12 南通龙洋水产有限公司 A kind of Fugu rubripes raun and fugu obscurus milter cross breeding method

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CN102187839B (en) * 2011-06-02 2012-08-08 中国水产科学研究院淡水渔业研究中心 Artificial crossing cultivation method of seawater takifugu species
CN103416325B (en) * 2012-05-15 2014-10-15 上海市水产研究所 Technique of rearing fry of obscure puffer in low salinity sea water

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