CN104931418A - High-luminous-intensity light source microscope and image identifying and analyzing device and application - Google Patents
High-luminous-intensity light source microscope and image identifying and analyzing device and application Download PDFInfo
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- CN104931418A CN104931418A CN201510325995.3A CN201510325995A CN104931418A CN 104931418 A CN104931418 A CN 104931418A CN 201510325995 A CN201510325995 A CN 201510325995A CN 104931418 A CN104931418 A CN 104931418A
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Abstract
The invention relates to a high-luminous-intensity light source microscope and image identifying and analyzing device and an application. The microscope comprises a built-in high-luminous-intensity LED light collecting light source device. The built-in high-luminous-intensity LED light collecting light source device comprises a light source, a brightness regulator, a built-in high-luminous-intensity LED light source, a light collecting cover and a lamp seat. The luminous-intensity of the LED light source is controlled by the brightness regulator, and the needed intensity of light rays is provided for sample identifying and analyzing. The high-luminous-intensity light source microscope image identifying and analyzing device comprises the high-luminous-intensity light source microscope, a camera and an image analyzing system. When the device is applied to fiber identifying and component analyzing and testing, imaging of a fiber structure form is clear, the measuring accuracy is high, the good fiber identifying and analyzing effects can be obtained, and an existing optical projector method can be replaced.
Description
Technical field
The invention belongs to microscope to differentiate and fields of measurement, particularly a kind of high luminous intensity light source microscope and image authentication analytical equipment and application.
Background technology
Traditional transmission-type sample microscope artificial light generally adopts incandescent lamp, its shortcoming is that luminescence efficiency is low, generate heat two large, object sample is seen in dark to dyed, be difficult to differentiate its structural form by microscope or microscopic projector, such as, in the mixed textile processed of the dyeing cashmere that price is higher, cashmere content component measures the ratio being difficult to come discriminatory analysis cashmere and mixed fibre thereof from configuration of surface, brings difficulty to the microscope discrimination method of Textile Fibre Product.To the measure that this problem is taked be at present, in advance dull fiber chemical reagent is faded and improve the penetrability of sample, but the method except formality is loaded down with trivial details, spended time longer except, chemical reagent process makes fiber size expand or shrinks the error producing measurement result.
Fibre identification and proximate analysis can adopt optical microscopy, longitudinal and the cross-sectional structure morphological feature with microscopic examination fiber, the standard photographs of control fiber identifies the classification of unknown fiber, different fiber difference is come, measure diameter or sectional area respectively and count radical, thus calculating the weight percent content of each group of fiber.At present, domestic examination of fibers mechanism is in discriminating and measure fibre diameter and sectional area and often use optics microscopic projector, project in instrument screen after fiber fragment is amplified 500 times, with by the scale dipstick metering place fiber width in the screen center of circle or measure fibre diameter with wedge shape ruler, successively record measurement result, obtain fibre diameter mean value.There is subject matter in the method, must measure in darkroom, manual operation is more, and imaging definition is inadequate in addition, not strong to fiber surface structure resolution characteristic, is particularly difficult to identify to the darker fiber surface structure form of dyeing.
Summary of the invention
The object of the present invention is to provide a kind of high luminous intensity light source microscope and image authentication analytical equipment and application, mainly solve the defect existing for above-mentioned existing technology, adopt built-in high luminous intensity LED and add the new microscope light source special construction of photomask, with microscope, camera and Graphic System combination, form a novel high luminous intensity light source MIcrosope image discriminatory analysis device, adopting this device to improve measurand identification is significantly imaging definition and penetrating light intensity, when application is tested with fibre identification and proximate analysis, fibre structure form imaging clearly, measuring accuracy is high, be particularly useful for solving current transmission-type microscope to differentiate dark object specimen surface structural form and the difficulty of proximate analysis, the development of microscope discriminatory analysis technology is made to have had new breakthrough.
To achieve these goals, technical scheme of the present invention is:
A kind of high luminous intensity light source microscope, is characterized in that: the built-in high luminous intensity LED light harvesting light supply apparatus that it comprises; Described built-in high luminous intensity LED light harvesting light supply apparatus comprises power supply, brightness regulator, built-in high luminous intensity LED light source, photomask, lamp socket; Built-in high luminous intensity LED light source photomask center line light harvesting focus place, is packed in microscope internal base by lamp socket, and its issued light line produces the directional light of bunchy by photomask; Control built-in high luminous intensity LED light source by brightness regulator luminous strong and weak, and provide required light intensity by photomask to sample discriminatory analysis.
Described built-in high luminous intensity LED light harvesting light supply apparatus, is characterized in that: with radiator structure on described built-in high luminous intensity LED light harvesting light source.
A kind of high luminous intensity light source MIcrosope image discriminatory analysis device, is characterized in that: it comprises microscope and the camera of above-mentioned high luminous intensity light source; The microscope of described high luminous intensity light source also comprises diaphragm, condenser, objective table, object lens and lens barrel; Built-in high luminous intensity LED light source issued light line is by being irradiated on the sample above objective table after photomask, diaphragm, condenser; Described camera is placed on lens barrel.
Described high luminous intensity light source MIcrosope image discriminatory analysis device, it is characterized in that: described camera is transferred to computing machine by data line the sample structure morphological image absorbed, and is arranged on image authentication analytic system in computing machine and allows sample structure form be presented in real time on indicator screen.
Described high luminous intensity light source MIcrosope image discriminatory analysis device, it is characterized in that: described screen presents pure and fresh specimen surface structural form, judge the variety classification of sample thus, and by being arranged on image authentication analytic system in computing machine, the every structural form index of sample can be measured.
Described high luminous intensity light source MIcrosope image discriminatory analysis device, is characterized in that: described computing machine also connects input-output device further.
High luminous intensity light source microscopy images discriminatory analysis device as described above is used for fibre identification and a proximate analysis, it is characterized in that: it comprises the steps:
A, open the power supply of high luminous intensity light source microscopy images discriminatory analysis device and computing machine:
B, high-ranking officers' fiducial mark chi are placed on objective table, and moving stage makes scale to field of view center, rotate the accurate focus knob of microscope thickness and make figure focus;
C, with standard scale 100 micrometer length for benchmark, the micron number that on automatic calibration indicator screen, each pixel is corresponding;
D, take off calibration scale, put sample to be tested, after focusing, rotate brightness regulator, regulate built-in high luminous intensity LED light source light intensity, light arrives the sample of objective table by diaphragm, condenser;
E, after above-mentioned focusing and brightness regulation, tested sample imaging is input in computing machine through camera and data line, sample longitudinal direction or cross sectional image is clearly presented on indicator screen through being arranged on image authentication analytic system in computing machine;
F, mechanism's morphological feature according to various fiber, on recognition screen, the classification of unknown fiber samples to be tested, separates different classes of pars fibrosa;
G, structural form feature according to various fiber, on recognition screen, the classification of unknown fiber samples to be tested, separates different classes of pars fibrosa;
H, measuring specimen finish or area by being arranged in computing machine image authentication analytic system, automatically calculating the weight percent content of the mean diameter of fiber, diameter distribution and each component fiber.
Compared with prior art advantage of the present invention is as follows:
1, the high luminous intensity light source microscopy images discriminatory analysis device of the present invention's design, it adopts new type light source, solve the problem existing for external Equipment Microscopic incandescent lighting, improve imaging definition during measurand identification and penetrating light intensity, improve the ability of microscopic method identification and measurement.
2, apparatus of the present invention also can directly be measured without the need to fading dyed dark sample.Can various dyeing and dark sample morphosis be identified, when the current dark sample of customer service is differentiated, the problem of fading and processing and just can see clearly existing for specimen surface form must be adopted in advance, avoid chemical reagent process to produce specimen size and change the measuring error brought.
3, apparatus of the present invention are when being applied to fibre identification and proximate analysis tests, fibre structure form imaging clearly, and measuring accuracy is high, obtains higher fibre identification and analytical effect, can replace existing optical projector method.
Accompanying drawing explanation
Fig. 1 is the Novel light source structure schematic diagram of apparatus of the present invention.
In Fig. 1,1-power supply, 2-brightness regulator, 3-built-in high luminous intensity LED light source, 4-photomask, 41-focus, 5-lamp socket.
Fig. 2 is high luminous intensity light source MIcrosope image discriminatory analysis device schematic diagram of the present invention.
In Fig. 2,1-power supply, 2-brightness regulator, 3-built-in high luminous intensity LED light source, 4-photomask, 41-focus, 5-lamp socket, 6-diaphragm, 7-condenser, 8-objective table, 9-sample, 10-object lens, 11-lens barrel, 12-camera, 13-data line, 14-computing machine, 15-display.
Embodiment
Refer to Fig. 1-2, the invention discloses a kind of high luminous intensity light source MIcrosope image discriminatory analysis device.As shown in Figure 1, main innovate point of the present invention is: the built-in high luminous intensity LED light harvesting light supply apparatus that microscope comprises; Described built-in high luminous intensity LED light harvesting light supply apparatus comprises power supply 1, brightness regulator 2, built-in high luminous intensity LED light source 3, photomask 4, lamp socket 5, built-in high luminous intensity LED light source 3 are positioned at photomask 4 center line light harvesting focus 41 place, be packed in microscope internal base by lamp socket 5, its issued light line produces the directional light of bunchy by photomask 4; Control built-in high luminous intensity LED light source 3 by brightness regulator 2 luminous strong and weak, and differentiate and the light intensity analyzed needed for greatest service to sample by photomask 4.
As a kind of optimal way, described built-in high luminous intensity LED light source 3 is with radiator structure.
As shown in Figure 2, this luminous intensity light source MIcrosope image discriminatory analysis device of the present invention comprises microscope and the camera 12 of high luminous intensity light source as above.The microscope of described high luminous intensity light source also comprises diaphragm 6, condenser 7, objective table 8, object lens 10 and lens barrel 11; Built-in high luminous intensity LED light source 3 issued light line by be irradiated to after photomask 4, diaphragm 6, condenser 7 above objective table 8 with in raising 9; Described camera 12 is placed on lens barrel 11.
Referring to Fig. 2, described camera 12 is placed on the microscopical lens barrel 11 of new type light source, by data line 13 absorbing the sample 9 structural form graphics transport that arrives to computing machine, the figure discriminatory analysis system in computing machine 14 that is arranged on allows sample 9 structural form be presented in real time on display 15 screen.Described screen presents clearly sample 9 surface structure form, judge the variety classification of sample thus, and by being arranged on image authentication analytic system in computing machine 14, the every structural form index of sample can be measured.Described camera 12 is connected with computing machine 14, and computing machine also connects input-output device further.
For fibre identification and proximate analysis, a kind of method of testing adopting high luminous intensity light source MIcrosope image discriminatory analysis device as above to realize, it comprises the steps:
A, open the power supply of high luminous intensity light source microscopy images discriminatory analysis device and computing machine:
B, high-ranking officers' fiducial mark chi are placed on objective table, and moving stage makes scale to field of view center, rotate the accurate focus knob of microscope thickness and make figure focus;
C, with standard scale 100 micrometer length for benchmark, the micron number that on automatic calibration indicator screen, each pixel is corresponding;
D, take off calibration scale, put sample to be tested, after focusing, rotate brightness regulator, regulate built-in high luminous intensity LED light source light intensity, light arrives the sample of objective table by diaphragm, condenser;
E, after above-mentioned focusing and brightness regulation, tested sample imaging is input in computing machine through camera and data line, sample longitudinal direction or cross sectional image is clearly presented on indicator screen through being arranged on image authentication analytic system in computing machine;
F, mechanism's morphological feature according to various fiber, on recognition screen, the classification of unknown fiber samples to be tested, separates different classes of pars fibrosa;
G, structural form feature according to various fiber, on recognition screen, the classification of unknown fiber samples to be tested, separates different classes of pars fibrosa;
H, measuring specimen finish or area by being arranged in computing machine image authentication analytic system, automatically calculating the weight percent content of the mean diameter of fiber, diameter distribution and each component fiber.
In the inventive method implementation process, highlight strength MIcrosope image discriminatory analysis device used adopts high-power LED light source to be placed in photomask focal position and forms high brightness equality light beam, enter the optical microscope optics decorum and irradiate fiber samples through diaphragm, collecting lens, by the ccd image unit of object lens amplification imaging in camera, be illustrated on indicator screen with making tested fiber samples clear picture by Computer Image Processing software, the new embodied in combination of this high luminous intensity LED light harvesting light source and the microscopic image analysis system cynapse advantage of following several respects:
1, the luminosity of LED light harvesting light source is large with existing incandescent source, makes the test of novel optical microscope discriminatory analysis dark object component become possibility.
2, LED light harvesting light source thermal value is far below incandescent lamp thermal value used at present, makes microscope built-in light source not because long-time use causes the large infringement apparatus structure of its thermal value.
3, the shorter wavelength of LED light harvesting light source, is conducive to the resolution improving optical microscope.
4, LED light harvesting light source shortcoming is under the surround of a comparison field of producing high brightness, the harmful effect that can cause observer's eyesight when observing fiber imaging by sight, and the blue light composition in LED light source may cause Shanghai to retina, but the present invention adopts the combination of LED light harvesting light source and MIcrosope image system, successfully solve this problem.
Therefore, feature of the present invention is the combined measuring device of LED high luminous intensity light harvesting light source and microscopic image analysis system, solving current transmissive optical microscope cannot differentiate in dark object structural form and proximate analysis, the application of particularly succeeding in textile woollen and cashmere component is differentiated and analyzed, under the imaging of fibre structure form is clear, measuring accuracy is high, obtains good fibre identification and analytical effect, can replace existing optical projector method.
Be only present pre-ferred embodiments in sum, be not used for limiting practical range of the present invention.Namely all equivalences done according to the content of the present patent application the scope of the claims change and modify, and all should be technology category of the present invention.
Claims (7)
1. high luminous intensity light source microscope and image authentication analytical equipment and an application, is characterized in that: the built-in high luminous intensity LED light harvesting light supply apparatus that it comprises; Described built-in high luminous intensity LED light harvesting light supply apparatus comprises power supply (1), brightness regulator (2), built-in high luminous intensity LED light source (3), photomask (4), lamp socket (5); Built-in high luminous intensity LED light source (3) is positioned at photomask (4) center line light harvesting focus (41) place, is packed in microscope internal base by lamp socket (5), and its issued light line produces the directional light of bunchy by photomask (4); Control built-in high luminous intensity LED light source (3) by brightness regulator (2) luminous strong and weak, and provide required light intensity by photomask (4) to sample discriminatory analysis.
2. built-in high luminous intensity LED light harvesting light supply apparatus according to claim 1, is characterized in that: with radiator structure on described built-in high luminous intensity LED light source (3).
3. high luminous intensity light source microscope and image authentication analytical equipment and an application image discriminatory analysis device, is characterized in that: it comprises microscope and the camera (12) of high luminous intensity light source as claimed in claim 1 or 2; The microscope of described high luminous intensity light source also comprises diaphragm (6), condenser (7), objective table (8), object lens (10) and lens barrel (11); Built-in high luminous intensity LED light source (3) issued light line is by being irradiated on the sample (9) of objective table (8) top after photomask (4), diaphragm (6), condenser (7); Described camera (12) is placed on lens barrel (11).
4. high luminous intensity light source MIcrosope image discriminatory analysis device according to claim 3, it is characterized in that: described camera (12) absorbing sample (9) the structural form image transmitting that arrives to computing machine (14) by data line (13), is arranged on the interior image authentication analytic system of computing machine (14) and allows sample (9) structural form be presented in real time on display (15) screen.
5. high luminous intensity light source MIcrosope image discriminatory analysis device according to claim 4, it is characterized in that: described screen presents pure and fresh sample (9) surface structure form, judge the variety classification of sample thus, and by being arranged on the interior image authentication analytic system of computing machine (14), the every structural form index of sample can be measured.
6. high luminous intensity light source MIcrosope image discriminatory analysis device according to claim 4, is characterized in that: described computing machine (14) also connects input-output device further.
7. the high luminous intensity light source microscopy images discriminatory analysis device as described in claim 3 or 4 or 5 or 6 is used for fibre identification and a proximate analysis, it is characterized in that: it comprises the steps:
Open the power supply of high luminous intensity light source microscopy images discriminatory analysis device and computing machine:
B, high-ranking officers' fiducial mark chi are placed on objective table (8), and moving stage makes scale to field of view center, rotate the accurate focus knob of microscope thickness and make figure focus;
C, with standard scale 100 micrometer length for benchmark, the micron number that on automatic calibration display (15) screen, each pixel is corresponding;
D, take off calibration scale, put sample to be tested, after focusing, rotate brightness regulator (2), regulate built-in high luminous intensity LED light source (3) light intensity, light arrives the sample (9) of objective table (8) by diaphragm (6), condenser (7);
E, after above-mentioned focusing and brightness regulation, tested sample (9) imaging is input in computing machine (14) through camera (12) and data line (13), is clearly presented on display (9) screen through being arranged on image authentication analytic system in computing machine (14) by sample (9) longitudinal direction or cross sectional image;
F, mechanism's morphological feature according to various fiber, on recognition screen, the classification of unknown fiber samples (9) to be tested, separates different classes of pars fibrosa;
G, structural form feature according to various fiber, on recognition screen, the classification of unknown fiber samples (9) to be tested, separates different classes of pars fibrosa;
H, measuring specimen finish or area by being arranged on the interior image authentication analytic system of computing machine (14), automatically calculating the weight percent content of the mean diameter of fiber, diameter distribution and each component fiber.
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