CN104928209A - Paenibacillus and application thereof - Google Patents

Paenibacillus and application thereof Download PDF

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CN104928209A
CN104928209A CN201510248846.1A CN201510248846A CN104928209A CN 104928209 A CN104928209 A CN 104928209A CN 201510248846 A CN201510248846 A CN 201510248846A CN 104928209 A CN104928209 A CN 104928209A
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series bacillus
sulfur dioxide
paenibacillus
filler
gas
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CN104928209B (en
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李琳
刘俊新
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Research Center for Eco Environmental Sciences of CAS
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    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/46—Removing components of defined structure
    • B01D53/48—Sulfur compounds
    • B01D53/50—Sulfur oxides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84—Biological processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2251/00—Reactants
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/30—Sulfur compounds
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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Abstract

The invention discloses a Paenibacillus and application thereof to removal of gas containing sulfur dioxide. The strain is specifically Paenibacillus sp. IB1, and the preservation number is CGMCC No.10775. The Paenibacillus sp. IB1 has very strong acid resistance, can continuously and effectively purify sulfur dioxide in an environment with very strong acidity (pH2), can adapt to high-temperature conditions, can still grow well at 50-65 DEG C and can effectively remove sulfur dioxide in high-temperature waste gas. Bacterium agents prepared by using the strain and microbial active fillers prepared by loading bacterium suspension on porous fillers can be used for treating waste gas containing sulfur dioxide, reach 90-100 percent in sulfur dioxide degrading efficiency, and has the characteristics of short startup time and high loading capacity.

Description

One strain series bacillus and application thereof
Technical field:
The invention belongs to field of environmental improvement, be specifically related to a strain series bacillus and removing containing the application in form waste gas of sulfur dioxide.
Background technology:
The process discharge such as fired coal combustion, Metal smelting, flue gas acid preparing, mud indirectly drying are in a large number containing the waste gas of sulfurous gas.Atmospheric sulfur dioxide exceeds standard, and not only ecotope is caused to having a strong impact on, and has high risks to HUMAN HEALTH.In addition, sulfur dioxide emissions enter air formed acid rain can cause significant damage to Architectural Equipment.All there is strict restriction countries in the world to the discharge of sulfurous gas, and the regulation of China to sulfur dioxide emissions is also more and more stricter.
Compared with other physical chemistry waste gas processing method, bioremediation has the advantages such as cost is low, easy and simple to handle, technology clean, non-secondary pollution because of it, becomes the focus of research after the nineties.Biological ratio juris is the process utilizing the biological oxidation of microorganism to be vitriol by Sulphur Dioxide.
But, the usual apposition growth of microorganism at carrier surface, need when running bio-reactor regularly to carrier spraying nutrient solution to maintain nutrient needed for microbial growth and moisture.In the process of spray, microorganism very easily comes off from carrier surface, flows out, cause loss with unnecessary nutritive medium from the bio-reactor of purifying exhaust air, causes the problem that the treatment effect of sulfurous gas reduces.Further, carrier usually adopt, gac, haydite, polyurethane sponge etc.
The inoculum purifying the bio-reactor containing sulfurous gas mostly is the active sludge of sewage work, and the microorganism adaptive phase is long, and bio-reactor starts slow.Desulfurization bacterium after inoculation domestication enrichment, can shorten the start time of biological desulphurization reactor.But in the microorganism of testing laboratory's domestication enrichment, competitive capacity is weak, in actual applications, is usually replaced by the microorganism that competitive capacity is strong.
Under aerobic condition, sulfurous gas, under the effect of desulfurization bacterium, is converted into vitriol and H +(such as formula 1), owing to there being H +produce, the pH value in reactor is usually below 3, but a lot of desulfurization bacterium is as the not acid resistance such as Pseudomonadaceae sp., Thiobacillus sp., and needing to add ealkaline buffer in use procedure regulates pH, maintains the steady running of reactor.
What discharge from fired coal combustion, Metal smelting, flue gas acid preparing, mud indirectly drying contains form waste gas of sulfur dioxide, and typical temperature is at 50-200 DEG C.But common desulfurization bacterium optimum temperuture is 20-35 DEG C, non-refractory, in actual applications, needs before bio-reactor, increase gas cooling installation, reduces inlet air temperature, to meet microbial growth condition.Like this, add facility investment and working cost, and complicated operation.
2SO 2+O 2+2H 2O→2SO 4 2-+4H +(1)
Summary of the invention:
Easily coming off in carrier surface load to solve microorganism in prior art, starting slowly, the problem of acid-resistant and anti-high-temperature poor performance, the invention provides a strain series bacillus (Paenibacillus sp.) IB1, and this bacterial strain is applied to removes containing in form waste gas of sulfur dioxide.
Series bacillus (Paenibacillus sp.) IB1 involved in the present invention, for obtaining isolate from Ecological Environment Research Center, Chinese Academy of Sciences's water pollution control laboratory treatment containing in the reactor of form waste gas of sulfur dioxide.This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center with on April 30th, 2015, deposit number is CGMCC No.10775, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101.
This bacterial strain feature is as follows:
Series bacillus cell is shaft-like, peritrichous, Gram-positive.In physical environment, competitive capacity is strong, can produce siderophore with other microorganism compete when iron level is extremely low, by grabbing amino acid, carbohydrate, and secretes the antibacterial substance such as microbiotic and lyase acquisition competitive edge.Wide accommodation, can extensively utilize carbohydrate, amino acid, organic acid for carbon source, at strong acid (pH2-3), all can grow under the extreme conditions such as high temperature (50 DEG C-65 DEG C), can life-time service and the physical condition adapting to Various Complex.
Optimum growth temperature is 25-30 DEG C, pH is 6.5-7.0.
Another problem to be solved by this invention is to provide series bacillus (Paenibacillus sp.) IB1 and is removing containing the application in form waste gas of sulfur dioxide.
By series bacillus IB1 by test tube slant kind-shaking flask kind-seeding tank-production tank-product (packaging formulation is liquid bacterial agent or the solid absorption microbial inoculum) microbial inoculum that production technique prepares, be 10-3000m at gas flow 3/ h, residence time 0.5-2.0min, temperature 35-55oC, sulfur dioxide concentration is 20-200mg/m 3, sulfurous gas removal efficiency can reach more than 90%.Can be used for purifying the waste gas containing sulfurous gas, solve the sulfur dioxide pollution problem that the processes such as fired coal combustion, Metal smelting, flue gas acid preparing produce.
The Another application of described series bacillus (Paenibacillus sp.) IB1 its bacteria suspension is attached to the microorganism active filler that on protruded packing, preparation removes containing form waste gas of sulfur dioxide, better processes S-contained substance.The filler prepared by this bacterium is 0.001-200m at microorganism active packing volume 3, the inlet gas concentration of sulfurous gas is 20-200mg/m 3, gas flow rate is 0.05-10000m 3/ h, purification temperature be under 30-65 DEG C of condition to containing form waste gas of sulfur dioxide purification efficiency reach more than 90%.
Described filler is one or more in polyurethane foamed blocks, granulated active carbon, volcanics, perlite;
Described packing density is 10-25kg/m 3, porosity is 45-95%, and particle diameter is 8-50mm;
The mass ratio of described bacteria suspension and light porous filler is 10-100: 10;
In described microorganism active filler, series bacillus IB1 cell concentration is greater than 10 6cFU/g (active filler).
Concrete preparation process is as follows:
(1) slant culture: cultivate and prepare series bacillus IB1 slant strains;
Described series bacillus IB1 slant medium consists of: glucose 0.5g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeSO 47H 2o 0.01g, Na 2s 2o 35H 2o 5.0g, agar 20g, water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min.
(2) preparation of bacteria suspension: series bacillus IB1 inclined-plane thalline step (1) prepared is by 5-20% inoculum size fermentation culture step by step, and being separated and obtaining cell concentration is 0.3-0.8g/L series bacillus IB1 bacteria suspension;
Series bacillus IB1 collecting cells method is as follows;
Series bacillus IB1 inclined-plane thalline presses 5-20% inoculum size fermentation culture step by step, culture temperature 36 DEG C, incubation time 48h, ventilation 5-200L/min, dissolved oxygen >2.0mg/L, tank pressure 0.03-0.10Mpa, fermented liquid is placed in refrigerated centrifuge in 4 DEG C, 2000-8000rpm, centrifugal concentrating 5-20min, abandoning supernatant, precipitation sterile nutrient solution dilutes, and obtaining cell concentration is 0.3-0.8g/L series bacillus IB1 bacteria suspension;
Consisting of of described series bacillus IB1 liquid fermentation medium: glucose 0.5g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeSO 47H 2o 0.01g, water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min, are cooled to room temperature and add 5.0g/LNa again after sterilizing 2s 2o 35H 2o;
Consisting of of described series bacillus IB1 sterile nutrient solution: glucose 0.5g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeCl 27H 2o 0.01g, water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min;
(3) preparation of microorganism active filler:
A microbial bacteria suspension prepared by step (3) is sprayed the surface of the light porous filler of packet or particle shape by (); B protruded packing block (or particle) with series bacillus IB1 is put into impeller and is rotated mixing by (); C (), again to light porous filling surface spray microbial bacteria suspension, obtain microorganism active filler, 4 DEG C save backup;
Preferably, repeating step (a)-(c) 2-3 time, makes equal appendix in material surface and micropore have mycetocyte, obtains microorganism active filler.
Beneficial effect:
1. series bacillus IB1 provided by the invention has the efficiency of higher removal sulfurous gas, can reach 90-100%; And the adaptive phase is short, in 1 week, DeR can be started fast, be applied in purification containing in the waste gas bio-reactor of sulfurous gas, can the starting period be shortened.
2. series bacillus IB1 competitive capacity is strong, siderophore can be produced and other microorganism competes when iron level is extremely low, by grabbing amino acid, carbohydrate, and the secretion antibacterial substance such as microbiotic and lyase (antimicrobial substances) obtains competitive edge, the decontamination effect improving of sulfurous gas steady in a long-term can be maintained.
3. series bacillus IB1 has very strong acid resistance, can in the environment of acidity very strong (pH2) the purification sulfurous gas of continuous and effective.
4. series bacillus IB1 can adapt to hot conditions, still can well-grown at 50-65 DEG C, and effectively removes the sulfurous gas in comparatively high temps waste gas.
Accompanying drawing illustrates:
Fig. 1 series bacillus (Paenibacillus sp.) IB1 phylogenetic tree
Embodiment:
Embodiment 1: strain identification
The bacterial strain DNA adopting Full-automatic magnetic beads instrument for extracting nucleic acid Smart LabAssist-16 and respective environment sample DNA extraction purification test kit (Taiwan round dot Nai meter technical concern company limited) to screen acquisition respectively carries out Isolation and purification, select bacterial universal primers F16S-27 and R16S-1492 to carry out pcr amplification, primer sequence is respectively:
F16S-27(5`-AGAGTTTGATCCTGGCTCAG-3`)
R16S-1492(5`-CGGTTACCTTGTTACGACTTC-3`)
PCR reaction conditions is set as: 94 DEG C of denaturation 5min; 94 DEG C of sex change 30s subsequently, 56 DEG C of annealing 30s, 72 DEG C extend 90s, circulate 25 cycles; Then 72 DEG C extend 10min; Last 4 DEG C keep 10min.Then PCR primer is connected on PMD-19T carrier (the precious biological company limited in Dalian), is transformed into subsequently in bacillus coli DH 5 alpha competent cell (the precious biological company limited in Dalian).Competent cell 50 μ l after transforming is coated on the LB substratum containing galactoside, sec.-propyl-β-d-thiogalactoside and penbritin.Cultivate after 12 hours, carry out LB liquid medium cultivation from each dull and stereotyped picking 25 single bacterial plaques of white.Cultivate after 2 hours, nutrient solution is entrusted and carries out sequencing analysis in Hua Da gene company limited.First the sequence recorded adopts DNAMAN software to carry out similarity merger, sequence merger similarity being greater than 97% is an operating unit, the representative sequence chosen in each operating unit utilizes the 16SrDNA gene order in BLAST and GenBank database to carry out homology analysis, choose more than 1400bp length to compare (Clustelx1.83), adopt ortho position to connect (Neighbour Joining) method and carry out Phylogenetic Analysis (MEGA3.1).Phylogenetic tree as shown in Figure 1.
Embodiment 2: a kind ofly remove microbe stuffing containing sulfur dioxide gas and preparation thereof
A kind of microorganism active filler removed containing sulfur dioxide gas, described filler appendix has series bacillus (Paenibacillus sp.) IB1 bacteria suspension, described series bacillus IB1, deposit number is CGMCCNo.10775, and described filler is polyurethane foamed blocks;
Described packing density is 15kg/m 3, porosity is 90%, and particle diameter is 20mm;
Described series bacillus IB1 with the form appendix of bacteria suspension on filler;
Described series bacillus IB1 bacteria suspension and the mass ratio of filler are 60: 10;
In described microorganism active filler, series bacillus IB1 cell concentration is 1.1 × 10 6cFU/g;
Described microorganism active filler preparation process is as follows:
(1) slant culture: prepare series bacillus (Paenibacillus sp.) IB1 slant strains;
Described series bacillus IB1 slant medium consists of: glucose 0.5g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeSO 47H 2o 0.01g, Na 2s 2o 35H 2o 5.0g, agar 20g, water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min, are cooled to room temperature and add 5.0g/L Na again after sterilizing 2s 2o 35H 2o.
(2) preparation of bacteria suspension: series bacillus IB1 inclined-plane thalline 10% inoculum size fermentation culture step by step, being separated and obtaining cell concentration is 0.5g/L series bacillus IB1 bacteria suspension;
Series bacillus IB1 collecting cells method is as follows;
Series bacillus IB1 inclined-plane thalline is by 10% inoculum size fermentation culture step by step, culture temperature 36 DEG C of incubation time 48h, ventilation 100L/min, dissolved oxygen >2.0mg/L, tank pressure 0.06Mpa, fermented liquid is placed in refrigerated centrifuge in 4 DEG C, 5000rpm, centrifugal concentrating 10min, abandoning supernatant, precipitation sterile nutrient solution dilutes, and obtaining cell concentration is 0.5g/L series bacillus IB1 bacteria suspension;
Consisting of of described series bacillus IB1 liquid fermentation medium: glucose 0.5g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeSO 47H 2o 0.01g, water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min; Be cooled to room temperature after sterilizing and add 5.0g/LNa again 2s 2o 35H 2o.
Consisting of of described series bacillus IB1 sterile nutrient solution: glucose 0.5g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeCl 27H 2o 0.01g water 1000ml, pH value 6.8-7.2; 121 DEG C of sterilizing 20min;
(3) preparation of microorganism active filler: the microbial bacteria suspension that step (2) is prepared by (a) sprays the surface of polyurethane foam by pump and spray header; Spray rate is 10L/min, and spray time is 60min; Stir, stirring velocity is 100rpm simultaneously, and churning time is 100min; Bacteria suspension is fully contacted with light porous; B polyurethane foam with series bacillus IB1 is put into impeller by (), rotate mixing 50min under 10rpm rotating speed; Along with the rotation of mixing tank, the mycetocyte being attached to light porous material surface enters into pore interior due to the effect of centrifugal force; C () to polyurethane foam surface spraying microbial bacteria suspension, stirs again simultaneously; Obtain microorganism active filler, 4 DEG C save backup;
Repeating step (a)-(c) 3 times, makes equal appendix in material surface and micropore have mycetocyte, obtains microorganism active filler;
Described rotary blender is the revolution of efficient fine container, stirring-type mixing equipment, for the dryness of different ratios or the Homogeneous phase mixing of moist particulate matter, and in mixing process, does not produce melting, the volatilization or rotten of material.Rotary blender main body is that stainless material makes, and volume is 0.05-10m 3, inside and outside wall polishing, without dead angle, built-in agitating vane, external rotating adds that built-in blade stirs, light porous filler and bacteria suspension radial roller in impeller, simultaneously also can horizontal and upper and lower convection current, and mixing efficiency is high.In mixing machine rotary course, on the one hand each component substances is fully mixed, on the other hand, due to the effect of centrifugal force, mycetocyte can enter in the hole of porous material inside, not only make the mycetocyte appendix amount of microorganism active filler high, and mycetocyte not easily runs off.
Embodiment 3: a kind ofly remove microbe stuffing containing sulfur dioxide gas and preparation thereof
A kind of microorganism active filler removed containing sulfur dioxide gas, described filler appendix has series bacillus (Paenibacillus sp.) IB1 bacteria suspension, described series bacillus IB1, deposit number is CGMCCNo.10775, and described filler is volcanics;
Described packing density is 25kg/m 3, porosity is 55%, and particle diameter is 10mm;
Described series bacillus IB1 with the form appendix of bacteria suspension on filler;
Described series bacillus IB1 bacteria suspension and the mass ratio of filler are 36: 10;
In described microorganism active filler, series bacillus IB1 cell concentration is 3.5 × 10 6cFU/g;
Described microorganism active filler preparation process is as follows:
(1) slant culture: prepare series bacillus (Paenibacillus sp.) IB1 slant strains;
Described series bacillus IB1 slant medium consists of: glucose 0.5g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeSO 47H 2o 0.01g, Na 2s 2o 35H 2o 5.0g, agar 20g, water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min, are cooled to room temperature and add 5.0g/L Na again after sterilizing 2s 2o 35H 2o.
(2) preparation of bacteria suspension: series bacillus IB1 inclined-plane thalline 10% inoculum size fermentation culture step by step, being separated and obtaining cell concentration is 0.6g/L series bacillus IB1 bacteria suspension;
Series bacillus IB1 collecting cells method is as follows;
Series bacillus IB1 inclined-plane thalline is by 10% inoculum size fermentation culture step by step, culture temperature 36 DEG C of incubation time 48h, ventilation 100L/min, dissolved oxygen >2.0mg/L, tank pressure 0.06Mpa, fermented liquid is placed in refrigerated centrifuge in 4 DEG C, 5000rpm, centrifugal concentrating 10min, abandoning supernatant, precipitation sterile nutrient solution dilutes, and obtaining cell concentration is 0.6g/L series bacillus IB1 bacteria suspension;
Consisting of of described series bacillus IB1 liquid fermentation medium: glucose 0.5g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeSO 47H 2o 0.01g, water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min; Be cooled to room temperature after sterilizing and add 5.0g/LNa again 2s 2o 35H 2o.
Consisting of of described series bacillus IB1 sterile nutrient solution: glucose 0.5g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeCl 27H 2o 0.01g water 1000ml, pH value 6.8-7.2; 121 DEG C of sterilizing 20min;
(3) preparation of microorganism active filler: the microbial bacteria suspension that step (2) is prepared by (a) sprays the surface of volcanics by pump and spray header; Spray rate is 10L/min, and spray time is 60min; Stir, stirring velocity is 100rpm simultaneously, and churning time is 100min; Bacteria suspension is fully contacted with light porous; B volcanics with series bacillus IB1 is put into impeller by (), rotate mixing 50min under 10rpm rotating speed; Along with the rotation of mixing tank, the mycetocyte being attached to light porous material surface enters into pore interior due to the effect of centrifugal force; C () to volcanics surface spraying microbial bacteria suspension, stirs again simultaneously; Obtain microorganism active filler, 4 DEG C save backup;
Repeating step (a)-(c) 3 times, makes equal appendix in material surface and micropore have mycetocyte, obtains microorganism active filler;
Described rotary blender is the revolution of efficient fine container, stirring-type mixing equipment, for the dryness of different ratios or the Homogeneous phase mixing of moist particulate matter, and in mixing process, does not produce melting, the volatilization or rotten of material.Rotary blender main body is that stainless material makes, and volume is 0.05-10m 3, inside and outside wall polishing, without dead angle, built-in agitating vane, external rotating adds that built-in blade stirs, light porous filler and bacteria suspension radial roller in impeller, simultaneously also can horizontal and upper and lower convection current, and mixing efficiency is high.In mixing machine rotary course, on the one hand each component substances is fully mixed, on the other hand, due to the effect of centrifugal force, mycetocyte can enter in the hole of porous material inside, not only make the mycetocyte appendix amount of microorganism active filler high, and mycetocyte not easily runs off.
Embodiment 4. 1 kinds removes the microorganism active filler containing sulfur dioxide gas,
A kind of microorganism active filler removed containing sulfur dioxide gas, described filler appendix has series bacillus (Paenibacillus sp.) IB1 bacteria suspension, described series bacillus IB1, deposit number is CGMCCNo.10775, and described filler is activated carbon granule;
Described packing density is 10kg/m 3, porosity is 65%, and particle diameter is 30mm;
Described series bacillus IB1 with the form appendix of bacteria suspension on filler;
Described series bacillus IB1 bacteria suspension and the mass ratio of filler are 90: 10;
In described microorganism active filler, series bacillus IB1 cell concentration is 8.0 × 10 6cFU/g
Described microorganism active filler preparation process is as follows:
(1) slant culture: prepare series bacillus (Paenibacillus sp.) IB1 slant strains;
Described series bacillus IB1 slant medium consists of: glucose 0.5g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeSO 47H 2o 0.01g, Na 2s 2o 35H 2o 5.0g, agar 20g, water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min, are cooled to room temperature and add 5.0g/L Na again after sterilizing 2s 2o 35H 2o.
(2) preparation of bacteria suspension: series bacillus IB1 inclined-plane thalline 10% inoculum size fermentation culture step by step, being separated and obtaining cell concentration is 0.7g/L series bacillus IB1 bacteria suspension;
Series bacillus IB1 collecting cells method is as follows;
Series bacillus IB1 inclined-plane thalline is by 10% inoculum size fermentation culture step by step, culture temperature 36 DEG C of incubation time 48h, ventilation 100L/min, dissolved oxygen >2.0mg/L, tank pressure 0.06Mpa, fermented liquid is placed in refrigerated centrifuge in 4 DEG C, 5000rpm, centrifugal concentrating 10min, abandoning supernatant, precipitation sterile nutrient solution dilutes, and obtaining cell concentration is 0.7g/L series bacillus IB1 bacteria suspension;
Consisting of of described series bacillus IB1 liquid fermentation medium: glucose 0.5g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeSO 47H 2o 0.01g, water 1000ml, pH value 6.8-7.2,121 DEG C of sterilizing 20min; Be cooled to room temperature after sterilizing and add 5.0g/LNa again 2s 2o 35H 2o.
Consisting of of described series bacillus IB1 sterile nutrient solution: glucose 0.5g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeCl 27H 2o 0.01g water 1000ml, pH value 6.8-7.2; 121 DEG C of sterilizing 20min;
(the preparation of 3 microorganism active fillers: the microbial bacteria suspension that step (2) is prepared by (a) sprays the surface of activated carbon granule by pump and spray header; Spray rate is 10L/min, and spray time is 60min; Stir, stirring velocity is 100rpm simultaneously, and churning time is 100min; Bacteria suspension is fully contacted with light porous; B activated carbon granule with series bacillus IB1 is put into impeller by (), rotate mixing 50min under 10rpm rotating speed; Along with the rotation of mixing tank, the mycetocyte being attached to light porous material surface enters into pore interior due to the effect of centrifugal force; C () to activated carbon granule surface spraying microbial bacteria suspension, stirs again simultaneously; Obtain microorganism active filler, 4 DEG C save backup;
Preferred repeating step (a)-(c) 3 times, makes equal appendix in material surface and micropore have mycetocyte, obtains microorganism active filler;
Described rotary blender is the revolution of efficient fine container, stirring-type mixing equipment, for the dryness of different ratios or the Homogeneous phase mixing of moist particulate matter, and in mixing process, does not produce melting, the volatilization or rotten of material.Rotary blender main body is that stainless material makes, and volume is 0.05-10m 3, inside and outside wall polishing, without dead angle, built-in agitating vane, external rotating adds that built-in blade stirs, light porous filler and bacteria suspension radial roller in impeller, simultaneously also can horizontal and upper and lower convection current, and mixing efficiency is high.In mixing machine rotary course, on the one hand each component substances is fully mixed, on the other hand, due to the effect of centrifugal force, mycetocyte can enter in the hole of porous material inside, not only make the mycetocyte appendix amount of microorganism active filler high, and mycetocyte not easily runs off.
Embodiment 5: a kind of microorganism active filler that utilizes removes method containing sulfur dioxide gas
(1) there is the microorganism active filler of series bacillus IB1 to be placed in a container embodiment 2 gained appendix, pass into SO 2gas take sulfur dioxide gas as sulphur source, activation microorganism active filler; Soak time is 7 days;
The concentration of described sulfurous gas is 80mg/m 3;
(2) the microorganism active filler after activation is placed in bio-reactor, passes into the waste gas containing sulfurous gas, with in series bacillus IB1 purifying exhaust air containing sulfurous gas, microorganism active packing volume is 100m 3, the inlet gas concentration of sulfurous gas is 100mg/m 3, gas flow rate is 1000m 3/ h, purification temperature is 35 DEG C; Regularly maintain the activity of microorganism active filler to microorganism active filling surface spraying nutrient solution, spray frequency is spray 1 time for 3 days, and each spray rate is 50L/min, and each spray time is 60min;
Consisting of of described nutritive medium: glucose 0.5g, yeast powder 0.2g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeCl 27H 2o 0.01g, water 1000ml, pH 6.8-7.2; Tracing detection is containing the degradation capability of sulfurous gas.
Embodiment 6: experiment effect
Be 80mg/m by embodiment 2 gained filler through concentration 3sulphur dioxide activating 7d after for the process of following gas:
(1) process is containing the waste gas of sulfurous gas, and microorganism active packing volume is 7.5m 3, sulfurous gas mean air entry concentration be 95mg/m 3, gas flow is 300m 3/ h, the residence time is 90s, operates under 35 DEG C of conditions, regularly maintains the activity of microorganism active filler to microorganism active filling surface spraying nutrient solution, and spray frequency is spray 1 time for 3 days, and each spray rate is 50L/min, and each spray time is 60min;
Consisting of of described nutritive medium: glucose 0.5g, yeast powder 0.2g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeCl 27H 2o 0.01g, water 1000ml, pH 6.8-7.2.
Bio-reactor runs August continuously, pH 2-3, and the mean air entry concentration of sulfurous gas is: 95mg/m 3, concentration of giving vent to anger is 10mg/m 3, lower than the atmosphere pollutants emission standards that country formulates, clearance reaches 90%.
Contrast and experiment shows: when not adding ferro element in nutritive medium, after reactor runs 1 month, series bacillus IB1 quantity is from 1.1 × 10 6cFU/g is increased to 2.5 × 10 6cFU/g.After add the nutritive medium containing 0.002g/L ferro element to bio-reactor, after reactor runs 1 month, series bacillus IB1 quantity is by 2.5 × 10 6cFU/g is increased to 3.7 × 10 7cFU/g, increases significantly.After 3 months, series bacillus IB1 quantity maintains 5.0 × 10 7-9.5 × 10 7cFU/g.Visible, series bacillus IB1 can keep quantity and activity for a long time, enables the permanently effective sulfurous gas removed in waste gas of bio-reactor.
(2) what produce for the treatment of sludge drying contains form waste gas of sulfur dioxide, and microorganism active packing volume is 70m 3, the inlet gas concentration of sulfurous gas is 65mg/m 3, gas flow is 3000m 3/ h, the residence time is 85s, gas temperature 55-60 DEG C, regularly maintains the activity of microorganism active filler to microorganism active filling surface spraying nutrient solution, and spray frequency is spray 1 time for 4 days, and each spray rate is 40L/min, and each spray time is 60min;
Consisting of of described nutritive medium: glucose 0.5g, yeast powder 0.2g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeCl 27H 2o 0.01g, water 1000ml, pH 6.8-7.2.
The clearance of sulfurous gas is close to 100%, and emission gases reaches the atmosphere pollutants emission standards that country formulates.
Equipment ran more than 6 months, pH 3-5, and series bacillus quantity maintains 1.08 × 10 7-2.21 × 10 8cFU/g, while reaching purification object, equipment can steady in a long-termly run.
(3) process is containing the waste gas of sulfurous gas, and microorganism active packing volume is 0.01m 3, the inlet gas concentration of sulfurous gas is 150mg/m 3, gas flow is 10L/min, and the residence time is 60s, 40 DEG C of operations, regularly maintain the activity of microorganism active filler to microorganism active filling surface spraying nutrient solution, spray frequency is spray 1 time for 5 days, each spray rate is 50L/min, and each spray time is 60min;
Consisting of of described nutritive medium: glucose 0.5g, yeast powder 0.2g, KH 2pO 42.0g, NaHCO 32.0g, KNO 32.0g, NH 4cl 0.5g, MgCl 26H 2o 0.5g, FeCl 27H 2o 0.01g, water 1000ml, pH 6.8-7.2.
Bio-reactor runs December continuously, pH 2-3, and concentration of giving vent to anger is 0-8mg/m 3, lower than the atmosphere pollutants emission standards that country formulates, average removal rate: 95%.In reactor, series bacillus quantity is 2.5 × 10 7-4.3 × 10 7cFU/g, bio-reactor is stable.
In above-mentioned gas treating processes, the starting period of bio-reactor is one week.And under equal conditions, after the bacterial classification in filler is replaced with pseudomonas, the starting period is 2 weeks-January.

Claims (3)

1. a strain series bacillus, is characterized in that, described series bacillus is series bacillus (Paenibacillus sp.) IB1, deposit number CGMCC.No.10775.
2. a strain series bacillus according to claim 1, is characterized in that: the efficiency that described series bacillus (Paenibacillus sp.) IB1 purifies containing form waste gas of sulfur dioxide reaches more than 90%.
3. a strain series bacillus according to claim 1 contains the application in form waste gas of sulfur dioxide in purification.
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