CN104892097A - Liquid culture medium of selenium-rich edible fungus and preparation method for liquid culture medium - Google Patents

Liquid culture medium of selenium-rich edible fungus and preparation method for liquid culture medium Download PDF

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Publication number
CN104892097A
CN104892097A CN201510236114.0A CN201510236114A CN104892097A CN 104892097 A CN104892097 A CN 104892097A CN 201510236114 A CN201510236114 A CN 201510236114A CN 104892097 A CN104892097 A CN 104892097A
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selenium
nutrient medium
liquid nutrient
edible mushroom
enriched edible
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CN201510236114.0A
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Chinese (zh)
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蔡军峰
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Ge Jiawu Bio Tech Ltd Suzhou
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Ge Jiawu Bio Tech Ltd Suzhou
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Abstract

The invention discloses a liquid culture medium of selenium-rich edible fungus. Every 1L of the culture medium comprises: 3.5g of magnesium sulfate, 22g of a nitrogen source, 28g of a carbon source, 2.0g of monopotassium phosphate, 3.5g of sodium hydrogen phosphate, 0.5g of vitamin B1, 3g of astragalus, 0.08g of selenomethionine, 0.05g of malt selenium and 2.5g of expanded perlite. The invention further discloses a preparation method for the liquid culture medium. The liquid culture medium provided by the invention is scientific in proportioning, rich in nutrition, high in selenium content and good in air permeability and water retention; the edible fungus cultured by the liquid culture medium is high in selenium content, good in quality, high in yield and capable of effectively improving the immunity of a human body; and the preparation method is simple, low in cost, non-toxic and beneficial to environmental pollution.

Description

Liquid nutrient medium of a kind of selenium-enriched edible mushroom and preparation method thereof
Technical field:
The present invention relates to edible mushrooms culture technique field, be specifically related to a kind of liquid nutrient medium of selenium-enriched edible mushroom.
Background technology:
The edible mushroomss such as needle mushroom, Pleurotus eryngii, crab mushroom have fine and tender taste, soft cunning, delicious flavour, protein content advantages of higher, deeply like by numerous people, edible fungi growth mainly relies on and absorb the nutrient of substratum and grow.The cultural method of existing culturing edible fungus is the mix and blend that added water by culture medium raw material.Culture medium prescription nutritive props is more unreasonable, causes that edible fungi growth is irregular, collection period extends, degradation problem under quality.
In addition, along with the progress of society, people yearn for the dietetic life of good health and a long life, and people have had higher understanding to edible mushrooms, have had higher requirement to the cultivating method of traditional edible mushrooms.In recent years, plant rich in selenium was subject to people and extensively favored.Selenium is the important component part of some antioxidases and selenium-P albumen in human body and animal, play a part balance oxidation reduction atmosphere in vivo, research proves that selenium has the effect improving animal immunizing power and preventing cancer, one of trace element being considered to needed by human, also adds trace element fertilizer in Chang Zuowei animal-feed trace mineral supplement or vegetable fertilizer.Therefore, the research of selenium-enriched edible mushroom becomes study hotspot.
Summary of the invention:
The object of this invention is to provide a kind of liquid nutrient medium of selenium-enriched edible mushroom, its proportioning science, nutritious; selenium content is high; ventilative good water-retaining property, the rich selenium content of the edible mushrooms cultivated by this liquid nutrient medium is high, and quality is good; output is high; effectively can improve the immunizing power of human body, and its preparation method is simple, cost is low; nontoxic, be conducive to environment protection.
Another object of the present invention is to provide the preparation method of this liquid nutrient medium.
For achieving the above object, the present invention is by the following technical solutions:
A liquid nutrient medium for selenium-enriched edible mushroom, every 1L substratum comprises:
Magnesium sulfate 3.5g, nitrogenous source 22g,
Carbon source 28g, potassium primary phosphate 2.0g,
Sodium hydrogen phosphate 3.5g, vitamins B 10.5g,
Radix Astragali 3g, Sethotope 0.08g,
Fructus Hordei Germinatus selenium 0.05g, pearlstone 2.5g.
Preferred as technique scheme, described nitrogenous source is the mixture of agar, shrimping beam trawl, rape shell, soybean cake powder.
Preferred as technique scheme, described carbon source is the mixture of glucose, Semen Maydis powder, starch.
Preferred as technique scheme, the size of described pearlstone is 50-80nm, and its water content is 5-8%.
A preparation method for the liquid nutrient medium of selenium-enriched edible mushroom, comprises the following steps:
(1) by the potato decortication in nitrogenous source, be cut into silk, add 1L water, add the shrimping beam trawl in nitrogenous source, rape shell, soybean cake powder and the Radix Astragali, continue after boiling to boil 15-20min, be cooled to room temperature, by 6-8 layer filtered through gauze, obtain filtrate, add agar, heat with slow fire and stir until agar dissolves, obtaining mixed solution;
(2) carbon source, magnesium sulfate, potassium primary phosphate, sodium hydrogen phosphate, vitamins B is added in the mixed solution obtained to step (1) 1, Fructus Hordei Germinatus selenium, Sethotope, continue slow fire boil 20-30min, add pearlstone, stir, obtain the liquid nutrient medium of selenium-enriched edible mushroom.
Preferred as technique scheme, in step (1), described in boil after continue to boil 20min.
Preferred as technique scheme, in step (2), the described simmered time is 28min.
Preferred as technique scheme, the pH of gained liquid nutrient medium is 10-11.
Compared with prior art, the present invention has following beneficial effect:
In liquid nutrient medium provided by the invention, add the nontoxic material being rich in selenium element such as Sethotope, Fructus Hordei Germinatus selenium to replace Sodium Selenite, be conducive to environment protection, and the selenium element that edible mushrooms can be provided abundant, make the edible mushrooms cultivated, selenium content is high, is conducive to the immunizing power improving human body;
Pearlstone has cell texture, and adsorptivity is strong, can provide the place of edible fungi growth, and the nutritive substance be supplied to required for edible fungi growth continued, effectively improve the Quality and yield of edible mushrooms.
Embodiment:
For a better understanding of the present invention, below by embodiment, the present invention is further described, and embodiment, only for explaining the present invention, can not form any restriction to the present invention.
Embodiment 1
A liquid nutrient medium for selenium-enriched edible mushroom, every 1L substratum comprises:
Magnesium sulfate 3.5g, nitrogenous source 22g, carbon source 28g, potassium primary phosphate 2.0g, sodium hydrogen phosphate 3.5g, vitamins B 10.5g, Radix Astragali 3g, Sethotope 0.08g, Fructus Hordei Germinatus selenium 0.05g, pearlstone 2.5g.
Its preparation method comprises the following steps:
(1) by the potato decortication in nitrogenous source, be cut into silk, add 1L water, add the shrimping beam trawl in nitrogenous source, rape shell, soybean cake powder and the Radix Astragali, continue after boiling to boil 15min, be cooled to room temperature, by 6-8 layer filtered through gauze, obtain filtrate, in filtrate, add agar, heat with slow fire and stir until agar dissolves, obtaining mixed solution;
(2) carbon source, magnesium sulfate, potassium primary phosphate, sodium hydrogen phosphate, vitamins B is added in the mixed solution obtained to step (1) 1, Fructus Hordei Germinatus selenium, Sethotope, continue slow fire boil 20min, add pearlstone, stir, obtain the liquid nutrient medium of selenium-enriched edible mushroom.
Embodiment 2
A liquid nutrient medium for selenium-enriched edible mushroom, every 1L substratum comprises:
Magnesium sulfate 3.5g, nitrogenous source 22g, carbon source 28g, potassium primary phosphate 2.0g, sodium hydrogen phosphate 3.5g, vitamins B 10.5g, Radix Astragali 3g, Sethotope 0.08g, Fructus Hordei Germinatus selenium 0.05g, pearlstone 2.5g.
Its preparation method comprises the following steps:
(1) by the potato decortication in nitrogenous source, be cut into silk, add 1L water, add the shrimping beam trawl in nitrogenous source, rape shell, soybean cake powder and the Radix Astragali, continue after boiling to boil 20min, be cooled to room temperature, by 6-8 layer filtered through gauze, obtain filtrate, in filtrate, add agar, heat with slow fire and stir until agar dissolves, obtaining mixed solution;
(2) carbon source, magnesium sulfate, potassium primary phosphate, sodium hydrogen phosphate, vitamins B is added in the mixed solution obtained to step (1) 1, Fructus Hordei Germinatus selenium, Sethotope, continue slow fire boil 30min, add pearlstone, stir, obtain the liquid nutrient medium of selenium-enriched edible mushroom.
Embodiment 3
A liquid nutrient medium for selenium-enriched edible mushroom, every 1L substratum comprises:
Magnesium sulfate 3.5g, nitrogenous source 22g, carbon source 28g, potassium primary phosphate 2.0g, sodium hydrogen phosphate 3.5g, vitamins B 10.5g, Radix Astragali 3g, Sethotope 0.08g, Fructus Hordei Germinatus selenium 0.05g, pearlstone 2.5g.
Its preparation method comprises the following steps:
(1) by the potato decortication in nitrogenous source, be cut into silk, add 1L water, add the shrimping beam trawl in nitrogenous source, rape shell, soybean cake powder and the Radix Astragali, continue after boiling to boil 16min, be cooled to room temperature, by 6-8 layer filtered through gauze, obtain filtrate, in filtrate, add agar, heat with slow fire and stir until agar dissolves, obtaining mixed solution;
(2) carbon source, magnesium sulfate, potassium primary phosphate, sodium hydrogen phosphate, vitamins B is added in the mixed solution obtained to step (1) 1, Fructus Hordei Germinatus selenium, Sethotope, continue slow fire boil 22min, add pearlstone, stir, obtain the liquid nutrient medium of selenium-enriched edible mushroom.
Embodiment 4
A liquid nutrient medium for selenium-enriched edible mushroom, every 1L substratum comprises:
Magnesium sulfate 3.5g, nitrogenous source 22g, carbon source 28g, potassium primary phosphate 2.0g, sodium hydrogen phosphate 3.5g, vitamins B 10.5g, Radix Astragali 3g, Sethotope 0.08g, Fructus Hordei Germinatus selenium 0.05g, pearlstone 2.5g.
Its preparation method comprises the following steps:
(1) by the potato decortication in nitrogenous source, be cut into silk, add 1L water, add the shrimping beam trawl in nitrogenous source, rape shell, soybean cake powder and the Radix Astragali, continue after boiling to boil 17min, be cooled to room temperature, by 6-8 layer filtered through gauze, obtain filtrate, in filtrate, add agar, heat with slow fire and stir until agar dissolves, obtaining mixed solution;
(2) carbon source, magnesium sulfate, potassium primary phosphate, sodium hydrogen phosphate, vitamins B is added in the mixed solution obtained to step (1) 1, Fructus Hordei Germinatus selenium, Sethotope, continue slow fire boil 24min, add pearlstone, stir, obtain the liquid nutrient medium of selenium-enriched edible mushroom.
Embodiment 5
A liquid nutrient medium for selenium-enriched edible mushroom, every 1L substratum comprises:
Magnesium sulfate 3.5g, nitrogenous source 22g, carbon source 28g, potassium primary phosphate 2.0g, sodium hydrogen phosphate 3.5g, vitamins B 10.5g, Radix Astragali 3g, Sethotope 0.08g, Fructus Hordei Germinatus selenium 0.05g, pearlstone 2.5g.
Its preparation method comprises the following steps:
(1) by the potato decortication in nitrogenous source, be cut into silk, add 1L water, add the shrimping beam trawl in nitrogenous source, rape shell, soybean cake powder and the Radix Astragali, continue after boiling to boil 18min, be cooled to room temperature, by 6-8 layer filtered through gauze, obtain filtrate, in filtrate, add agar, heat with slow fire and stir until agar dissolves, obtaining mixed solution;
(2) carbon source, magnesium sulfate, potassium primary phosphate, sodium hydrogen phosphate, vitamins B is added in the mixed solution obtained to step (1) 1, Fructus Hordei Germinatus selenium, Sethotope, continue slow fire boil 26min, add pearlstone, stir, obtain the liquid nutrient medium of selenium-enriched edible mushroom.
Embodiment 6
A liquid nutrient medium for selenium-enriched edible mushroom, every 1L substratum comprises:
Magnesium sulfate 3.5g, nitrogenous source 22g, carbon source 28g, potassium primary phosphate 2.0g, sodium hydrogen phosphate 3.5g, vitamins B 10.5g, Radix Astragali 3g, Sethotope 0.08g, Fructus Hordei Germinatus selenium 0.05g, pearlstone 2.5g.
Its preparation method comprises the following steps:
(1) by the potato decortication in nitrogenous source, be cut into silk, add 1L water, add the shrimping beam trawl in nitrogenous source, rape shell, soybean cake powder and the Radix Astragali, continue after boiling to boil 19min, be cooled to room temperature, by 6-8 layer filtered through gauze, obtain filtrate, in filtrate, add agar, heat with slow fire and stir until agar dissolves, obtaining mixed solution;
(2) carbon source, magnesium sulfate, potassium primary phosphate, sodium hydrogen phosphate, vitamins B is added in the mixed solution obtained to step (1) 1, Fructus Hordei Germinatus selenium, Sethotope, continue slow fire boil 280min, add pearlstone, stir, obtain the liquid nutrient medium of selenium-enriched edible mushroom.
More than show and describe ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.

Claims (8)

1. a liquid nutrient medium for selenium-enriched edible mushroom, is characterized in that, every 1L substratum comprises:
Magnesium sulfate 3.5g, nitrogenous source 22g,
Carbon source 28g, potassium primary phosphate 2.0g,
Sodium hydrogen phosphate 3.5g, vitamins B 10.5g,
Radix Astragali 3g, Sethotope 0.08g,
Fructus Hordei Germinatus selenium 0.05g, pearlstone 2.5g.
2. the liquid nutrient medium of a kind of selenium-enriched edible mushroom as claimed in claim 1, is characterized in that: described nitrogenous source is the mixture of agar, shrimping beam trawl, rape shell, soybean cake powder.
3. the liquid nutrient medium of a kind of selenium-enriched edible mushroom as claimed in claim 1, is characterized in that: described carbon source is the mixture of glucose, Semen Maydis powder, starch.
4. the liquid nutrient medium of a kind of selenium-enriched edible mushroom as claimed in claim 1, is characterized in that: the size of described pearlstone is 50-80nm, and its water content is 5-8%.
5. the preparation method of the liquid nutrient medium of a kind of selenium-enriched edible mushroom as described in as arbitrary in Claims 1-4, is characterized in that: comprise the following steps:
(1) by the potato decortication in nitrogenous source, be cut into silk, add 1L water, add the shrimping beam trawl in nitrogenous source, rape shell, soybean cake powder and the Radix Astragali, continue after boiling to boil 15-20min, be cooled to room temperature, by 6-8 layer filtered through gauze, obtain filtrate, add agar, heat with slow fire and stir until agar dissolves, obtaining mixed solution;
(2) carbon source, magnesium sulfate, potassium primary phosphate, sodium hydrogen phosphate, vitamins B is added in the mixed solution obtained to step (1) 1, Fructus Hordei Germinatus selenium, Sethotope, continue slow fire boil 20-30min, add pearlstone, stir, obtain the liquid nutrient medium of selenium-enriched edible mushroom.
6. the preparation method of the liquid nutrient medium of a kind of selenium-enriched edible mushroom as claimed in claim 5, is characterized in that: in step (1), described in boil after continue to boil 20min.
7. the preparation method of the liquid nutrient medium of a kind of selenium-enriched edible mushroom as claimed in claim 5, is characterized in that: in step (2), and the described simmered time is 28min.
8. the preparation method of the liquid nutrient medium of a kind of selenium-enriched edible mushroom as claimed in claim 5, is characterized in that: the pH of gained liquid nutrient medium is 10-11.
CN201510236114.0A 2015-05-11 2015-05-11 Liquid culture medium of selenium-rich edible fungus and preparation method for liquid culture medium Pending CN104892097A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106431572A (en) * 2016-09-30 2017-02-22 桂林淮安天然保健品开发有限公司 Lucid-ganoderma cultivation method for increasing ganoderan and selenium content
CN106431571A (en) * 2016-09-30 2017-02-22 桂林淮安天然保健品开发有限公司 Ganoderma lucidum karst cultivation method for improving ganoderma lucidum polysaccharides and selenium content
CN106478236A (en) * 2016-09-30 2017-03-08 桂林淮安天然保健品开发有限公司 The Lucid ganoderma pocket planting culture medium of ganoderan and Se content can be improved
CN107417365A (en) * 2017-06-15 2017-12-01 蔡敬侠 A kind of mushroom culture medium and preparation method thereof
CN109354548A (en) * 2018-12-06 2019-02-19 安徽铜草花现代农业科技有限公司 A kind of culture medium of edible fungus preparation method full of nutrition

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CN101172902A (en) * 2007-10-23 2008-05-07 贵州大学 Method for formulating culture medium additive agent for cultivation of selenium-rich ganoderma lucidum
CN102138436A (en) * 2010-12-28 2011-08-03 湖北圣峰药业有限公司 Method for culturing selenium-enriched Cordyceps militaris
CN102150554A (en) * 2010-02-11 2011-08-17 王府 Culture medium and planting method of astragali mushroom
CN102204477A (en) * 2011-05-28 2011-10-05 镇江市食用菌研究所 Liquid culture medium for producing selenium-enriched Hericium erinaceus
CN103250568A (en) * 2013-05-30 2013-08-21 安徽鑫农食用菌有限公司 Method for preparing hericium erinaceus rich in selenium

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101172902A (en) * 2007-10-23 2008-05-07 贵州大学 Method for formulating culture medium additive agent for cultivation of selenium-rich ganoderma lucidum
CN102150554A (en) * 2010-02-11 2011-08-17 王府 Culture medium and planting method of astragali mushroom
CN102138436A (en) * 2010-12-28 2011-08-03 湖北圣峰药业有限公司 Method for culturing selenium-enriched Cordyceps militaris
CN102204477A (en) * 2011-05-28 2011-10-05 镇江市食用菌研究所 Liquid culture medium for producing selenium-enriched Hericium erinaceus
CN103250568A (en) * 2013-05-30 2013-08-21 安徽鑫农食用菌有限公司 Method for preparing hericium erinaceus rich in selenium

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106431572A (en) * 2016-09-30 2017-02-22 桂林淮安天然保健品开发有限公司 Lucid-ganoderma cultivation method for increasing ganoderan and selenium content
CN106431571A (en) * 2016-09-30 2017-02-22 桂林淮安天然保健品开发有限公司 Ganoderma lucidum karst cultivation method for improving ganoderma lucidum polysaccharides and selenium content
CN106478236A (en) * 2016-09-30 2017-03-08 桂林淮安天然保健品开发有限公司 The Lucid ganoderma pocket planting culture medium of ganoderan and Se content can be improved
CN107417365A (en) * 2017-06-15 2017-12-01 蔡敬侠 A kind of mushroom culture medium and preparation method thereof
CN109354548A (en) * 2018-12-06 2019-02-19 安徽铜草花现代农业科技有限公司 A kind of culture medium of edible fungus preparation method full of nutrition

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