CN104886571A - A plant extracted immune-enhancing refined substance and a preparation method thereof - Google Patents

A plant extracted immune-enhancing refined substance and a preparation method thereof Download PDF

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Publication number
CN104886571A
CN104886571A CN201510289842.8A CN201510289842A CN104886571A CN 104886571 A CN104886571 A CN 104886571A CN 201510289842 A CN201510289842 A CN 201510289842A CN 104886571 A CN104886571 A CN 104886571A
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China
Prior art keywords
extract
thing
mixing
fruit
raffinate
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CN201510289842.8A
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Chinese (zh)
Inventor
王恭顺
李承根
朴太杰
杨立
杨庆智
岳徐冬
李庆中
周翔
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安徽广生堂生物科技有限公司
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Priority to CN201510289842.8A priority Critical patent/CN104886571A/en
Publication of CN104886571A publication Critical patent/CN104886571A/en

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The present invention discloses a plant extracted immune-enhancing refined substance and a preparation method thereof, and belongs to the technical field of bioactive substance preparation. The refined substance consists of aronia melanocarpa extract (52%), radix ginseng rubra extract (16%), mixed Chinese herbal medicine extract (16%) and mixed fruit extract (16%) in mass percentage. Mixed extract of the above-mentioned aronia melanocarpa extract, radix ginseng rubra extract, mixed Chinese herbal medicine extract and mixed fruit extract is used to prepare the pharmaceutical refined substance with an immune-enhancing effect. In conclusion, the refined substance can impact granular leukocytes and phagocytes with bactericidal function and cells with virucidal function to produce media immunity and humoral immunity. The refined substance is safe without any side effects, and has the effect on recovering and enhancing immune function; and long-term taking the refined substance provides effects on resisting senility and ageing, and maintaining exuberant energy and stamina for middle-aged and elderly people.

Description

A kind of food-orginated compounds strengthens immune raffinate and preparation method thereof
Technical field
The invention belongs to bioactivator preparing technical field, be specifically related to a kind of food-orginated compounds and strengthen immune raffinate and preparation method thereof.
Background technology
Body immunity is regulated by autonomic nerve system and endocrine system, by humoral immune and cellular immunity from exosmosis, or repel inner generate non-oneself become to assign to maintain shape constancy.Change this individual system by certain reason, just produce the symptom such as immunodeficiency, hypoimmunity.Immunodeficiency causes virus infections, and cancer, glycosuria, the refractory disease such as sterile.
Recover immune shape constancy to improve immunity, people commonly use immunity function reinforcing agent, immunity function revivifier.The representative immunity function reinforcing agent of current use has human immunoglobulin(HIg).But in end user's immunoglobulin (Ig) process, often there is the side effects such as allergic reaction, heating, burnout, poor appetite, depilation, white blood cell minimizing.Immunity function revivifier is then to the almost no effect of antitumor activity, anti-AIDS.Therefore, people recover the validity of immune shape constancy making great efforts research raising immunity, the security material of being free from side effects again.
The present invention utilizes wild cherry plum to extract thing, red ginseng extracts thing, thing is extracted in Chinese herbal medicine mixing, fruit mixing is extracted the plant mixing such as thing and is extracted thing, confirm that it affects the propagation of siberian crabapple vitals spleen cell medium size lymphocyte, affect the voracious ability of granular leucocyte neutrophil leucocyte, affect the receptor of spleen cell, the research affecting the increase of anti-acid enzymatic activity has come.
Summary of the invention
A kind of food-orginated compounds is the object of the present invention is to provide to strengthen immune raffinate and preparation method thereof, this invention utilizes plant to mix and extracts thing, the raffinate that preparation brings back to life immunity function, enhancing immunity function has no side effect again, this raffinate can strengthen human health physique, has economic and social benefits significantly.
A kind of food-orginated compounds provided by the present invention strengthen the formation of immune raffinate and mass percent as follows: wild cherry plum extracts thing 52%, and red ginseng extracts thing 16%, and thing 16% is extracted in Chinese herbal medicine mixing, and thing 16% is extracted in fruit mixing.
The present invention utilizes wild cherry plum to extract thing, red ginseng extraction thing, Chinese herbal medicine mixing extraction thing and fruit to mix the mixing extraction thing extracting thing, the pharmaceutical raffinate of preparation enhancing immunization.
Wild cherry plum is anthocyanin and the maximum fruit of polyphenol content in plum class fruit, and the content of its anthocyanin is 5 times of blueberry, 10 times of mossberry, 20 times of raspberry, and has anticancer, anti-inflammatory usefulness.
Red ginseng is the evaporate to dryness product of perennial ginseng, to rebuilding one's physical constitution, burnout, poor appetite, vomiting, diarrhoea, improve lung kidney function and there is good action.Pharmacologically have corticocerebral excitement and suppression, balance, antifatigue, anti-aging, strengthen immunity, promote sexual gland, suppress hyperglycaemia, promote protein synthesis, maintain shape constancy, the effect such as anticancer, removing toxic substances, especially remarkable benefit in relieving fatigue, develop immunitypty, unimpeded blood flow.
Chinese herbal medicine mixing extract thing pharmacologically have anti-acid, drive hemostasis, invigorate blood circulation, enrich blood, dirty, anticancer, anti-obesity, anti-inflammatory, antifatigue by force, and strengthen immunity, improve blood flow, reduce the effects such as acidifying cholesterol.Chinese herbal medicine mixing extraction thing formation and quality proportioning are in table 1.
Table 1: formation and the quality proportioning thereof of thing are extracted in Chinese herbal medicine mixing
Fruit mixing extract thing pharmacologically have anti-acid, anticancer, anti-obesity, dirty, antifatigue, and develop immunitypty by force, increase red blood cell, hypotensive, reduce the effects such as acidifying cholesterol.Fruit mixing extraction thing formation and quality proportioning are in table 2.
Table 2: formation and the quality proportioning thereof of thing are extracted in fruit mixing
Preparation method's concrete steps that a kind of food-orginated compounds provided by the present invention strengthens immune raffinate are as follows:
(1) prepare wild cherry plum and extract thing:
First fresh wild cherry plum is cleaned, drop into and extract tank, add the pure water of fresh wild cherry plum 3 times of quality, extract after maintaining 4 hours in 95 DEG C of temperature, extracting thing is cooled to 40 DEG C to filter with microfilter (1 μm), is concentrated to 60 Brix degrees, maintains sterilization in 20 minutes in 90 DEG C of temperature, in the ratio mixing maltodextrin of 1:1, spraying dry obtains wild cherry plum and extracts thing.
(2) prepare red ginseng and extract thing:
First extra dry red wine ginseng is cleaned, be cut into 2 ~ 5mm section, drop into and extract tank, add the pure water that 8 times of quality joined by extra dry red wine, extract after maintaining 4 hours in 95 DEG C of temperature, extract thing and be cooled to 40 DEG C to filter with microfilter (1 μm), be concentrated to 60 Brix degrees, maintain sterilization in 20 minutes in 90 DEG C of temperature, in the ratio mixing maltodextrin of 1:1, spraying dry obtains red ginseng and extracts thing.
(3) prepare Chinese herbal medicine mixing and extract thing:
First dry for Chinese herbal medicine raw material is cleaned, be cut into 2 ~ 5mm section, drop into and extract tank, add the pure water of the dry raw material of Chinese herbal medicine 8 times of quality, extract after maintaining 4 hours in 95 DEG C of temperature, extract thing and be cooled to 40 DEG C to filter with microfilter (1 μm), be concentrated to 60 Brix degrees, maintain sterilization in 20 minutes in 90 DEG C of temperature, in the ratio mixing maltodextrin of 1:1, spraying dry obtains Chinese herbal medicine mixing and extracts thing.
(4) prepare fruit mixing and extract thing:
First cleaned by fresh fruit raw material, peak, grape fruit, orange, oranges and tangerines, kumquat and the stripping and slicing of bitter orange belt leather taken in fig, muskmelon, watermelon, persimmon, Kiwi berry, pomegranate, banana, lemon, the Chinese; Chinese chestnut, lichee, mangosteen, durian, coconut fruit and walnut peeling; Pawpaw, papaya, junket pears, mango, apple and pears stoning stripping and slicing; Muskmelon, pineapple peeling stripping and slicing; Strawberry, red grape, blue or green grape, tomato, rambutan, date, A Sayi berry, mossberry, blueberry, blackberry, blueberry and mulberries monoblock drop into and extract tank, add the pure water of fresh fruit raw material 3 times of quality, extract after maintaining 4 hours in 95 DEG C of temperature, extracting thing is cooled to 40 DEG C to filter with microfilter (1 μm), be concentrated to 60 Brix degrees, maintain sterilization in 20 minutes in 90 DEG C of temperature, in the ratio mixing maltodextrin of 3:7, spraying dry obtains fruit mixing and extracts thing.
(5) with agitator tank mixing wild cherry plum extract thing, red ginseng extracts thing, thing is extracted in Chinese herbal medicine mixing and fruit mixing is extracted thing and obtained dry mixing and extract raffinate.
(6) finally obtain food-orginated compounds with the mixing extraction raffinate that pulverizer pulverizing is dry and strengthen immune raffinate, require more than 100 meshes.
(7) microorganism checking: inspection requirements meets the following conditions: every kilogram of bacterial population less than 100, every kilogram of fungi number less than 100, coliform presents feminine gender, is contained in sealing bag or hermetically sealed can after product meets the requirements, and seals up for safekeeping for less than 4 DEG C.
Detailed description of the invention
1, food-orginated compounds of the present invention strengthens the usefulness experiment of immune raffinate:
1. animal used as test: kind: male white mice.
age in week: 5 week age.
quantity: 40 (control group 10, each 10 of experimental group a, b, c)
raise: common rearing conditions.
2. experimental technique:
Food-orginated compounds strengthens immune raffinate distilled water and dissolves, oral ten weeks.Control group and experimental group oral dose are in table 3.
Table 3: control group and experimental group oral dose
3. lymphocyte proliferation assay:
Use etherization white mouse, cut out spleen, be ground into end, lyse red blood cells, carry out cell proliferation experiment with the blue method of testing of ALMA.Result shows, cell proliferation significant difference, and the cell proliferation of c group is maximum, and be secondly b group and b group, control group is minimum.Show that plant mixing is extracted raffinate and taken more, lymphopoiesis is also more.
4. neutrophil leucocyte voracity experiment:
Employing chemiluminescence immune analysis method is tested, and result shows, and the voracious significant difference of neutrophil leucocyte, the effect of c group cell voracity is the most active, and be secondly b group and b group, control group is minimum.Show that food-orginated compounds strengthens immune raffinate and takes more, the effect of neutrophil leucocyte voracity also jumps more.
5. the receptor experiment of spleen cell:
Adopt the experiment of cell streaming multicolor analysis, result shows, lymphocyte receptor and major histocompatibility complex receptor enliven significant difference, c group shows the most active, and be secondly b group and b group, control group is minimum.Show that food-orginated compounds strengthens immune raffinate and takes more, the receptor effect of spleen cell also jumps more.
6. anti-acid enzyme experiment:
Cut out the liver of white mouse, add phosphate buffer, pulverize with homogenizer, then decompose by sonication, the activity of enzyme analysis.Result shows, the significant difference of anti-acid enzyme, and the display of c group is minimum, and be secondly b group and b group, control group is maximum.Show that food-orginated compounds strengthens immune raffinate and takes more, anti-acid enzyme effect is also larger.
(3) product is made:
The match ratio that food-orginated compounds of the present invention strengthens immune raffinate should account for 10 ~ 30% of finished product gross mass and be advisable, lower than 10% time difficulty produce a desired effect, higher than 30% time can produce efficacy of a drug safety problem.
Food-orginated compounds of the present invention strengthens immune raffinate, can manufacture the medicine strengthening immunity, can making powder, granule, tablet, capsule, injection, paste, emulsion etc., can make dextrin, cyclodextrin in order to transport facility.Convention can be manufactured by medicine, add additive lactose, glucose, sucrose, compound sugar, sorbierite, sweet mellow wine, xylitol, erythritol, maltitol, starch, alginates, edible gelatin, calcium phosphate, cellulose, methylcellulose, polyvinylpyrrolidone, ethyl-para-hydroxybenzoate, pure water etc.
Adult drops into 200 mg/kg, children's suitable decrement every day.
Food-orginated compounds of the present invention strengthens immune raffinate, also can manufacture the health food strengthening immunity, can manufacturing ring agent, powder, particle, preserved material, tablet, capsule, beverage etc., can manufacture dextrin, cyclodextrin in order to transport facility.Convention can be manufactured by health food, add additive spices, flavor enhancement, pigment, stabilizing agent, anticorrisive agent, pure water etc.
1. product example: manufacture syrup, this syrup specifically form and proportioning as follows:
Food-orginated compounds strengthens immune raffinate, 2g
Asccharin, 0.8g
Sugar, 25.4g
Glycerine, 8.0g
Spices, 0.04g
Ethanol, 4.0g
Sorbic acid, 0.4g
Distilled water, in right amount.
2. product example: manufacture health food, composition and the proportioning of this health food are as follows:
3, invention effect:
Utilize plant of the present invention to mix and extract thing, white mouse is tested, result display adds lymphocytic propagation, peroxide and hydrogen peroxide showed increased, mean the voracious ability that have impact on neutrophil leucocyte, and add the performance of lymphocytic cell surface receptor, the activity of anti-acid enzyme.Illustrate that product of the present invention impact has the voracious cell of granular leucocyte of sterilizing function, impact has cell medium immunity and the humoral immune of function of killing the virus.Product of the present invention has the strong point that safety has no side effect, the usefulness having recovery, strengthen immunity function, effective prevention and therapy pathogenic virus, A type h1n1 influenza, cancer, AIDS.The elderly long-term taking, have anti-ageing, anti-aging, keep vigorous energy and effect of muscle power.

Claims (2)

1. food-orginated compounds strengthens an immune raffinate, it is characterized in that the formation of this raffinate and mass percent as follows: wild cherry plum extract thing 52%, red ginseng extract thing 16%, Chinese herbal medicine mixing extract thing 16%, fruit mixing extract thing 16%, formation and the mass percent thereof of described Chinese herbal medicine mixing extraction thing are: cortex acanthopanacis 1.3%, camomile 1.4%, persimmon leaf 2.6%, lotus leaf 3.2%, ginseng 0.2%, glossy ganoderma 0.2%, longevity bamboo 1.7%, the tuber of dwarf lilyturf 1.6%, the root of large-flowered skullcap 1.6%, honeysuckle 0.3%, cassia bark 1.7%, tealeaves 0.8%, pawpaw 3.5%, ginkgo leaf 1.7%, fluffy art 2.6%, loguat leaf 3.5%, turmeric 1.6%, grass-leaved sweetflag 1.7%, pine needle 3.5%, universe ginger 0.8%, Artemisia capillaris 3.2%, hawthorn 3.5%, the radix paeoniae rubrathe 3.5%, silvervine 0.8%, the root bark of Chinese wolf-berry 0.8%, yellow paint 1.5%, Asian puccoon 1.6%, the red tuber of multiflower knotweed 1.8%, Gardenia 2.3%, glutinous rehmannia 2.7%, selfheal 2.7%, schizonepeta 2.3%, lotus seeds 1.4%, erythrina bark 3.5%, Radix Angelicae Sinensis 3.0%, the Radix Astragali 2.0%, Chinese yam 2.7%, trifoliate orange root branches and leaves 2.7%, Ligusticum wallichii 3.0%, dried orange peel 2.9%, the dried immature fruit of citron orange 1.3%, pseudo-ginseng 0.5%, the root of kudzu vine 1.6%, dandelion 1.6%, mulberry leaf 2.7%, the red sage root 3.0%, Radix Codonopsis 3.0%, Radix Glycyrrhizae 2.3%, the fruit of Chinese wolfberry 0.3%, the fruit of Chinese magnoliavine 0.3%, formation and the mass percent thereof of described fruit mixing extraction thing are: mango 1.3%, apple 2.5%, pears 3.8%, oranges and tangerines 5.0%, red grape 5.0%, orange 5.0%, lemon 1.8%, pineapple 6.6%, mulberries 4.5%, blackberry, blueberry 3.5%, muskmelon 5.0%, strawberry 2.0%, banana 10.0%, blueberry 1.2%, mossberry 0.7%, Ah's Say berry 1.3%, pomegranate 1.8%, Kiwi berry 5.0%, persimmon 5.0%, date 0.5%, watermelon 2.5%, walnut 0.5%, peak 2.4% taken in the Chinese, grape fruit 1.9%, coconut fruit 1.0%, muskmelon 2.5%, rambutan 1.3%, kumquat 2.5%, durian 0.3%, mangosteen 1.0%, tomato 2.5%, junket pears 1.1%, papaya 0.2%, pawpaw 1.5%, fig 1.5%, lichee 1.5%, Chinese chestnut 1.5%, blue or green grape 1.5%, bitter orange 1.3%.
2. described in claim 1, a kind of food-orginated compounds strengthens the preparation method of immune raffinate, it is characterized in that the method concrete steps are as follows:
(1) prepare wild cherry plum and extract thing:
First fresh wild cherry plum is cleaned, drop into and extract tank, add the pure water of described fresh wild cherry plum 3 times of quality, extract after maintaining 4 hours in 95 DEG C of temperature, extracting thing is cooled to 40 DEG C to filter with microfilter, is concentrated to 60 Brix degrees, maintains sterilization in 20 minutes in 90 DEG C of temperature, in the ratio mixing maltodextrin of 1:1, spraying dry obtains described wild cherry plum and extracts thing;
(2) prepare red ginseng and extract thing:
First extra dry red wine ginseng is cleaned, be cut into 2 ~ 5mm section, drop into and extract tank, add the pure water that 8 times of quality joined by described extra dry red wine, extract after maintaining 4 hours in 95 DEG C of temperature, extract thing and be cooled to 40 DEG C to filter with microfilter, be concentrated to 60 Brix degrees, maintain sterilization in 20 minutes in 90 DEG C of temperature, in the ratio mixing maltodextrin of 1:1, spraying dry obtains described red ginseng and extracts thing;
(3) prepare Chinese herbal medicine mixing and extract thing:
First dry for Chinese herbal medicine raw material is cleaned, be cut into 2 ~ 5mm section, drop into and extract tank, add the pure water of the dry raw material of described Chinese herbal medicine 8 times of quality, extract after maintaining 4 hours in 95 DEG C of temperature, extract thing and be cooled to 40 DEG C to filter with microfilter, be concentrated to 60 Brix degrees, maintain sterilization in 20 minutes in 90 DEG C of temperature, in the ratio mixing maltodextrin of 1:1, spraying dry obtains the mixing of described Chinese herbal medicine and extracts thing;
(4) prepare fruit mixing and extract thing:
First cleaned by fresh fruit raw material, peak, grape fruit, orange, oranges and tangerines, kumquat and the stripping and slicing of bitter orange belt leather taken in fig, muskmelon, watermelon, persimmon, Kiwi berry, pomegranate, banana, lemon, the Chinese; Chinese chestnut, lichee, mangosteen, durian, coconut fruit and walnut peeling; Pawpaw, papaya, junket pears, mango, apple and pears stoning stripping and slicing; Muskmelon, pineapple peeling stripping and slicing; Strawberry, red grape, blue or green grape, tomato, rambutan, date, A Sayi berry, mossberry, blueberry, blackberry, blueberry and mulberries monoblock drop into and extract tank, add the pure water of described fresh fruit raw material 3 times of quality, extract after maintaining 4 hours in 95 DEG C of temperature, extracting thing is cooled to 40 DEG C to filter with microfilter, be concentrated to 60 Brix degrees, maintain sterilization in 20 minutes in 90 DEG C of temperature, in the ratio mixing maltodextrin of 3:7, spraying dry obtains the mixing of described fruit and extracts thing;
(5) mix that described wild cherry plum extracts thing, described red ginseng extracts thing, thing is extracted in the mixing of described Chinese herbal medicine with agitator tank and the mixing of described fruit is extracted thing and obtained dry mixing and extract raffinate;
(6) with the mixing that described drying pulverized by pulverizer extract raffinate finally obtained food-orginated compounds strengthen immune raffinate, then carry out microorganism checking to meet the following conditions: every kilogram of bacterial population less than 100, every kilogram of fungi number less than 100, coliform presents feminine gender, check that after meeting the requirements, obtained food-orginated compounds being strengthened immune raffinate is contained in sealing bag or hermetically sealed can, seals up for safekeeping temperature less than 4 DEG C.
CN201510289842.8A 2015-05-29 2015-05-29 A plant extracted immune-enhancing refined substance and a preparation method thereof CN104886571A (en)

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CN105687422A (en) * 2016-03-02 2016-06-22 深圳福山生物科技有限公司 Raspberry composition and application thereof
CN107373659A (en) * 2017-08-30 2017-11-24 上海新肌生物科技有限公司 A kind of anti-saccharification formula and preparation method of the free radical generating source for removing 92%
US10568866B1 (en) * 2018-10-05 2020-02-25 Matthias W. Rath Composition and its use for increasing innate immune health

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CN105687422A (en) * 2016-03-02 2016-06-22 深圳福山生物科技有限公司 Raspberry composition and application thereof
CN105687422B (en) * 2016-03-02 2020-05-05 深圳福山生物科技有限公司 Raspberry composition and application thereof
CN107373659A (en) * 2017-08-30 2017-11-24 上海新肌生物科技有限公司 A kind of anti-saccharification formula and preparation method of the free radical generating source for removing 92%
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Application publication date: 20150909