CN104865098A - Macroalgae attaching organism sampling device and sampling method - Google Patents

Macroalgae attaching organism sampling device and sampling method Download PDF

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CN104865098A
CN104865098A CN201510260289.5A CN201510260289A CN104865098A CN 104865098 A CN104865098 A CN 104865098A CN 201510260289 A CN201510260289 A CN 201510260289A CN 104865098 A CN104865098 A CN 104865098A
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etting
collection bag
sample collection
macro
sampling apparatus
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刘永虎
陈勇
田涛
张皓宇
赵学伟
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Dalian Ocean University
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Dalian Ocean University
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Abstract

本发明公开一种大型藻类附着生物采样装置,其特征在于:所述的采样装置包括两个相互铰接的半圆形钢圈(1),在半圆形钢圈(1)上连接有网衣部分(2),网衣部分(2)包括直接与半圆形钢圈(1)连接的圆柱形的上网衣(3),上网衣(3)与圆台形的下网衣(4)连接,下网衣(4)的末端与圆柱形的样品收集袋(5)连接,样品收集袋(5)的直径小于上网衣(3)的直径,在样品收集袋(5)的底端还设置有阀门(6),且样品收集袋(5)由不透水材质制成。以及一种利用该种采样装置进行采样的方法。

The invention discloses a sampling device for macroalgae attached organisms, which is characterized in that: the sampling device includes two semicircular steel rings (1) hinged to each other, and a netting is connected to the semicircular steel rings (1). Part (2), the net part (2) includes a cylindrical upper net (3) directly connected with the semicircular steel ring (1), and the upper net (3) is connected with a conical lower net (4), The end of the lower net (4) is connected with a cylindrical sample collection bag (5), the diameter of the sample collection bag (5) is smaller than the diameter of the upper net (3), and a bottom end of the sample collection bag (5) is also provided with The valve (6), and the sample collection bag (5) are made of impermeable material. And a sampling method using the sampling device.

Description

大型藻类附着生物采样装置及采样方法Large algae attached organism sampling device and sampling method

技术领域 technical field

本发明涉及一种采样装置和采样方法,特别是一种大型藻类附着生物采样装置及采样方法。 The invention relates to a sampling device and a sampling method, in particular to a sampling device and a sampling method for macroalgae attached organisms.

背景技术 Background technique

大型藻类是非常重要的初级生产力系统,为整个生态系统提供了大量的能量和有机质,藻类经过光合作用固定无机碳转换为有机碳,对大气的CO2循环具有重要意义,有很多浮游生物和附着生物(附着在大型藻类上的生物)在大型藻类群落周围,因此分析大型藻类生态系统的附着生物对分析生态系统的能量流动和物质流动具有十分重要意义。 Macroalgae are very important primary productivity systems that provide a large amount of energy and organic matter for the entire ecosystem. Algae fix inorganic carbon into organic carbon through photosynthesis, which is of great significance to the CO2 cycle of the atmosphere. There are many plankton and attached organisms (Organisms attached to macroalgae) are around macroalgae communities, so analyzing macroalgae ecosystem attachment organisms is of great significance for analyzing the energy flow and material flow of the ecosystem.

发明内容 Contents of the invention

本发明是为了解决现有技术所存在的上述不足,提出一种结构简单,设计巧妙,成本低廉,使用方便的大型藻类附着生物采样装置,以及利用该种装置进行大型藻类附着生物采样的方法。 The present invention aims to solve the above-mentioned deficiencies in the prior art, and proposes a simple structure, ingenious design, low cost, and easy-to-use macroalgae sampling device and a method for sampling macroalgae by using the device.

本发明的技术解决方案是:一种大型藻类附着生物采样装置,其特征在于:所述的采样装置包括两个相互铰接的半圆形钢圈1,在半圆形钢圈1上连接有网衣部分2,网衣部分2包括直接与半圆形钢圈1连接的圆柱形的上网衣3,上网衣3与圆台形的下网衣4连接,下网衣4的末端与圆柱形的样品收集袋5连接,样品收集袋5的直径小于上网衣3的直径,在样品收集袋5的底端还设置有阀门6,且样品收集袋5由不透水材质制成。 The technical solution of the present invention is: a sampling device for macroalgae adherent organisms, characterized in that: the sampling device includes two semicircular steel rings 1 hinged to each other, and a net is connected to the semicircular steel rings 1. Garment part 2, the net garment part 2 comprises the cylindrical upper net garment 3 that is directly connected with the semicircular steel ring 1, the upper net garment 3 is connected with the lower net garment 4 of conical shape, and the end of the lower net garment 4 is connected with the cylindrical sample The collection bag 5 is connected, and the diameter of the sample collection bag 5 is less than the diameter of the upper garment 3, and a valve 6 is also arranged at the bottom of the sample collection bag 5, and the sample collection bag 5 is made of an impermeable material.

一种利用如上所述的采样装置进行大型藻类附着生物采样的方法,其特征在于:以两个半圆形钢圈1并拢的方式下水,将采样装置的半圆形钢圈1打开从大型藻类的顶部向根部的方向套在网衣部分2内,将大型藻类的根部割断后重新并拢半圆形钢圈1,将完整的大型藻类带回船上,将采样装置的网衣部分2在海水中晃动,使附在上网衣3和下网衣4内壁上的生物集中到样品收集袋5中,然后从采样装置中将大型藻类放入装有蒸馏水的清洁容器一中刷洗,经刷洗处理后的大型藻类放入密封袋中标签记录,将采样装置的网衣部分2放置到清洁容器二中,将清洁容器一中的水体通过半圆形钢圈1所形成的开口倒入网衣部分2中,将网衣部分2从清洁容器二中取出,并开启阀门6,将样品收集袋5中的水体转移到样品瓶中,并再次将网衣部分2在海水中晃动,使残留在上网衣3和下网衣4内壁上的生物集中到样品收集袋5中,并将这部分生物连同水体转移到同一个样品瓶中,向样品瓶中加入5%的甲醛固定液,并进行样品处理或种类鉴别操作。 A method for sampling macroalgae adherent organisms using the above-mentioned sampling device, characterized in that it is launched into the water with two semicircular steel rings 1 close together, and the semicircular steel ring 1 of the sampling device is opened to collect macroalgae. Put the top of the macroalgae in the netting part 2 towards the root, cut off the roots of the macroalgae and close the semicircular steel ring 1 again, bring the complete macroalgae back to the ship, put the netting part 2 of the sampling device in seawater Shaking, the organisms attached to the inner wall of the upper netting 3 and the lower netting 4 are concentrated into the sample collection bag 5, and then the macroalgae are put into a clean container with distilled water for scrubbing from the sampling device. Put the large algae into a sealed bag and record the label, put the net part 2 of the sampling device into the cleaning container 2, pour the water in the cleaning container 1 into the net part 2 through the opening formed by the semicircular steel ring 1 , the netting part 2 is taken out from the cleaning container two, and the valve 6 is opened, the water body in the sample collection bag 5 is transferred to the sample bottle, and the netting part 2 is shaken in seawater again to make the remaining water in the netting 3 and the organisms on the inner wall of the net clothing 4 are concentrated in the sample collection bag 5, and this part of organisms is transferred to the same sample bottle together with the water body, and 5% formaldehyde fixative is added to the sample bottle, and the sample is processed or sorted. Identify operations.

从采样装置下水至将大型藻类带回船上的全过程中都要进行摄像记录。 Cameras were used to document the entire process from the launch of the sampling device to the return of the macroalgae to the ship.

本发明同现有技术相比,具有如下优点: Compared with the prior art, the present invention has the following advantages:

本发明所公开的采样装置,其结构简单,设计巧妙,成本低廉,操作方便,利用它和本发明所公开的采样方法,能够快速、便捷的将附着在大型藻类上的生物收集起来,这样就能够通过分析大型藻类生态系统的附着生物的种类、数量等参数,进而分析大型藻类所处生态系统的能量流动和物质流动等信息。该装置制作工艺简单,制造成本低廉,该方法便捷、高效,因此可以说本发明所公开的技术方案具备了多种优点,特别适合于在本领域中推广应用,其市场前景十分广阔。 The sampling device disclosed in the present invention has simple structure, ingenious design, low cost and convenient operation. By using it and the sampling method disclosed in the present invention, the organisms attached to the macroalgae can be collected quickly and conveniently, so that By analyzing the parameters such as the type and quantity of attached organisms in the macroalgae ecosystem, the information such as energy flow and material flow of the ecosystem where the macroalgae is located can be analyzed. The manufacturing process of the device is simple, the manufacturing cost is low, and the method is convenient and efficient. Therefore, it can be said that the technical solution disclosed in the present invention has many advantages, and is particularly suitable for popularization and application in the field, and its market prospect is very broad.

附图说明 Description of drawings

图1为本发明实施例采样装置的结构示意图。 Fig. 1 is a schematic structural diagram of a sampling device according to an embodiment of the present invention.

具体实施方式 Detailed ways

下面将结合附图说明本发明的具体实施方式。如图1所示:一种大型藻类附着生物采样装置,它包括两个相互铰接的半圆形钢圈1,这两个半圆形钢圈1能够形成一个正圆,在半圆形钢圈1上连接有网衣部分2,网衣部分2包括直接与半圆形钢圈1连接的上网衣3,这个上网衣5呈圆柱形;上网衣3的底端与下网衣4连接,下网衣4呈圆台形;下网衣4的底端与不透水的样品收集袋5连接,样品收集袋5呈圆柱形,并且样品收集袋5的直径小于上网衣3的直径,同时在样品收集袋5的底端还设置有阀门6。 The specific implementation manner of the present invention will be described below with reference to the accompanying drawings. As shown in Fig. 1: a kind of macroalgae attached biological sampling device, it comprises two mutually hinged semicircular steel rings 1, these two semicircular steel rings 1 can form a perfect circle, in the semicircular steel ring 1 is connected with a net garment part 2, and the net garment part 2 includes a net garment 3 directly connected with the semicircular steel ring 1, and this net garment 5 is cylindrical; the bottom end of the net garment 3 is connected with the lower net garment 4, and the lower Net clothing 4 is conical shape; The bottom of lower net clothing 4 is connected with impermeable sample collection bag 5, and sample collection bag 5 is cylindrical, and the diameter of sample collection bag 5 is less than the diameter of upper clothing 3, simultaneously in sample collection. The bottom end of the bag 5 is also provided with a valve 6 .

一种利用如上所述的采样装置进行大型藻类附着生物采样的方法,首先以两个半圆形钢圈1并拢的方式下水,将采样装置的半圆形钢圈1打开,并从大型藻类的顶部向根部的方向将其套在网衣部分2内,将大型藻类的根部割断后重新并拢半圆形钢圈1,将完整的大型藻类带回船上,并且在整个采样过程中(即采样装置下水至将大型藻类采集到船上的过程中),都要进行摄像记录。 A method for sampling macroalgae adherent organisms by using the above-mentioned sampling device, firstly enter the water with two semicircular steel rings 1 close together, open the semicircular steel ring 1 of the sampling device, and collect the samples from the macroalgae Put it in the netting part 2 from the top towards the root, cut off the roots of the macroalgae and close the semicircular steel ring 1 again, bring the complete macroalgae back to the ship, and during the whole sampling process (i.e. the sampling device From launching to collecting macroalgae on board), video recording must be carried out.

然后将采样装置的网衣部分2在海水中晃动,使附在上网衣3和下网衣4内壁上的生物集中到样品收集袋5中,然后从采样装置中将大型藻类放入装有蒸馏水的清洁容器一中刷洗,目的是让附着在大型藻类上的生物被冲洗下来,经刷洗处理后的大型藻类放入密封袋中标签记录。 Then the net part 2 of the sampling device is shaken in seawater, so that the organisms attached to the inner wall of the upper net 3 and the lower net 4 are concentrated into the sample collection bag 5, and then the macroalgae are put into a container filled with distilled water from the sampling device. The purpose is to wash off the organisms attached to the macroalgae. After scrubbing, the macroalgae are put into a sealed bag and recorded on the label.

将采样装置的网衣部分2放置到清洁容器二中,将清洁容器一中的水体通过半圆形钢圈1所形成的开口倒入网衣部分2中,将网衣部分2从清洁容器二中取出,并开启阀门6,将样品收集袋5中的水体转移到样品瓶中,并再次将网衣部分2在海水中晃动,使残留在上网衣3和下网衣4内壁上的生物集中到样品收集袋5中,并将这部分生物连同水体转移到同一个样品瓶中,向样品瓶中加入5%的甲醛固定液,并进行样品处理或种类鉴别操作。 The net part 2 of the sampling device is placed in the clean container two, the water body in the clean container one is poured into the net part 2 through the opening formed by the semicircular steel ring 1, and the net part 2 is taken from the clean container two. Take it out, and open the valve 6, transfer the water body in the sample collection bag 5 to the sample bottle, and shake the netting part 2 in seawater again, so that the organisms remaining on the inner wall of the upper netting 3 and the lower netting 4 are concentrated into the sample collection bag 5, and transfer this part of organisms together with the water body into the same sample bottle, add 5% formaldehyde fixative solution to the sample bottle, and perform sample processing or species identification operations.

Claims (3)

1. a macro settled organism sampling apparatus, it is characterized in that: described sampling apparatus comprises two semicircle steel rings (1) be hinged, semicircle steel ring (1) is connected with etting part (2), etting part (2) comprises the columniform upper etting (3) be directly connected with semicircle steel ring (1), upper etting (3) is connected with the lower etting (4) of truncated cone-shaped, the end of lower etting (4) is connected with columniform sample collection bag (5), the diameter of sample collection bag (5) is less than the diameter of etting (3), valve (6) is also provided with in the bottom of sample collection bag (5), and sample collection bag (5) is made up of waterproof material.
2. one kind utilizes sampling apparatus as defined in claim 1 to carry out the method for macro settled organism sampling, it is characterized in that: water under the mode of closing up with two semicircle steel rings (1), the direction of being opened by the semicircle steel ring (1) of sampling apparatus from the top of macro to root is enclosed within etting part (2), again semicircle steel ring (1) is closed up after being cut off by the root of macro, complete macro is taken back on ship, the etting part (2) of sampling apparatus is rocked in the seawater, the biology be attached on etting (3) and lower etting (4) inwall is made to focus in sample collection bag (5), then macro is put into the cleaning container one that distilled water is housed from sampling apparatus to scrub, macro after scrubbing process puts into sealing bag label record, the etting part (2) of sampling apparatus is placed in cleaning container two, water body in cleaning container one is poured in etting part (2) by the opening that semicircle steel ring (1) is formed, etting part (2) is taken out from cleaning container two, and Open valve (6), water body in sample collection bag (5) is transferred in sample bottle, and again etting part (2) is rocked in the seawater, the biology remained on etting (3) and lower etting (4) inwall is made to focus in sample collection bag (5), and this part biology is transferred in same sample bottle together with water body, the formaldehyde immobile liquid of 5% is added in sample bottle, and carry out sample preparation or kind discriminating operation.
3. the method for sampling according to claim 2, is characterized in that: all will carry out shooting record in from water sampling apparatus to the overall process taken back by macro on ship.
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CN108593338B (en) * 2018-06-14 2023-12-15 西华师范大学 Multifunctional acquisition instrument for attached algae
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CN110257230B (en) * 2019-06-26 2024-12-06 水利部中国科学院水工程生态研究所 A collection device suitable for algae
CN116686537A (en) * 2023-05-18 2023-09-05 浙江省水利河口研究院(浙江省海洋规划设计研究院) A device for collecting and concentrating benthic algae

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