CN104849329A - Resveratrol measuring method by multi-wall carbon nanotube modified electrode - Google Patents
Resveratrol measuring method by multi-wall carbon nanotube modified electrode Download PDFInfo
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- CN104849329A CN104849329A CN201510229508.3A CN201510229508A CN104849329A CN 104849329 A CN104849329 A CN 104849329A CN 201510229508 A CN201510229508 A CN 201510229508A CN 104849329 A CN104849329 A CN 104849329A
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Abstract
The invention discloses a resveratrol measuring method by a multi-wall carbon nanotube modified electrode. Firstly the multi-wall carbon nanotube modified electrode is prepared; then oxidation peak current of resveratrol at a place with -0.36V is measured by a voltmeter-ammeter method with the multi-wall carbon nanotube modified electrode as a working electrode, a rotating platinum wire electrode as a counter electrode, and an Ag/AgCl (3mol/L KCl) electrode as a reference electrode, and the resveratrol is quantitatively calculated. The resveratrol measuring method by the multi-wall carbon nanotube modified electrode is simple, and the multi-wall carbon nanotube modified electrode is convenient to prepare and can be used repeatedly for multiple times; the detection speed is high, and the accuracy rate is high.
Description
Technical field
The invention belongs to field of electrochemical detection, be specifically related to a kind of method that Multiwalled Carbon Nanotubes Modified Electrode surveys resveratrol.
Background technology
Resveratrol is polyphenol compound, is mainly derived from the plants such as grape, giant knotweed, peanut, mulberry fruit.Resveratrol is the natural polyphenol class material that a kind of biologically active is very strong, is also called resvertrol, is the chemopreventive agent of tumour, is also to reduction platelet aggregation, the chemopreventive agent of prevention and therapy atherosclerotic, cardiovascular and cerebrovascular disease.The result of study of United States Department of Agriculture shows, also contains considerable resveratrol in peanut red coat and benevolence.The verified beneficial effect had angiocardiopathy and cancer of the experimental study of resveratrol.Resveratrol has obvious prevention effect to hormone-dependent neoplasm.Also can there is prevention effect to osteoporosis, acne and senile dementia, there is antiviral and immunoregulation effect.The anti-ageing enzyme of a kind of monomer of inside of human body is worked, and then plays the latent effect of the various age-related disease of prevention, prolongation expected life.
Carbon nano-tube is the another allotrope of the carbon found after C60, and its radial dimension is less, the external diameter of pipe generally in a few nanometer to tens nanometers, the internal diameter of pipe is less, and what have only has about 1nm; And its length is generally at micron order, length and diameter, than very large, can reach 103 ~ 106.Therefore, carbon nano-tube is considered to a kind of typical monodimension nanometer material.Carbon nano-tube is since being found by the mankind, and be just described as following material, be one of Disciplinary Frontiers of international scientific in recent years always.
The modified electrode that carbon nano-tube is made after suitably processing, shows very stable electrochemical action.Assay method mainly high performance liquid chromatography, gas chromatography, mass spectroscopy, capillary electrophoresis, simple oscillographic voltammetry etc. at present to resveratrol.But these methods otherwise reappearance not good enough, need expensive equipment, or detection sensitivity is too low.And adopt chemically modified electrode detection resveratrol to be good approach by electrochemical method.Utilize multilayer carbon nanotube Electrode selectivity to measure resveratrol herein, result is satisfactory.
Summary of the invention
The object of the invention is to, provide a kind of Multiwalled Carbon Nanotubes Modified Electrode to survey the method for resveratrol.
The invention provides a kind of method that Multiwalled Carbon Nanotubes Modified Electrode surveys resveratrol, comprise the following steps:
First glass-carbon electrode is polished with abrasive paper for metallograph, on sheet glass, mirror-smooth is polished to successively again with the alumina powder of 0.05 μm, all rinse with redistilled water after each polishing, then respectively at supersound washing 5min in absolute ethyl alcohol and redistilled water, obtain the glass-carbon electrode processed;
Pulverized by effective for single mortar, add concentrated hydrochloric acid ultrasonic disperse and remove wherein metallic catalyst, cleaning, to neutral, adds dense HNO
3, 140 DEG C of backflow 5h, after being cooled to room temperature, are washed till weakly acidic pH, after being placed in baking oven 120 DEG C of dryings, obtain multi-walled carbon nano-tubes;
Take above-mentioned multi-walled carbon nano-tubes 1mg and add 10ml DMF, ultrasonic 5 minutes, form black suspension;
Get black suspension described in 20 μ L, drop to the described glassy carbon electrode surface processed, dry under infrared lamp, obtain Multiwalled Carbon Nanotubes Modified Electrode;
Be working electrode with described Multiwalled Carbon Nanotubes Modified Electrode, revolving shape platinum electrode is to electrode, Ag/AgCl (3mol/L KCl) electrode is contrast electrode, get the Britton-Robinson buffer solution of pH2.0 in the electrolytic cell of 10.0mL, pass into nitrogen deoxygenation 10min, at 0 ~ 0.8V with cyclic voltammetry activated electrode until cyclic voltammetry curve stablize, then resveratrol liquid to be measured is added, speed is swept with 100mV/s under nitrogen protection condition, the cyclic voltammetry curve of record 0 ~ 0.8V, measure the oxidation peak current at-0.36V place, for the quantitative test of resveratrol.
Beneficial effect of the present invention is: Multiwalled Carbon Nanotubes Modified Electrode of the present invention surveys the method for resveratrol, and method is simple, and Multiwalled Carbon Nanotubes Modified Electrode is easy to prepare, can repeatedly Reusability, and detection speed is fast, and accuracy rate is high.
Accompanying drawing explanation
Figure 1 shows that Multiwalled Carbon Nanotubes Modified Electrode of the present invention surveys the detection voltammogram of the method for resveratrol.
Embodiment
Hereafter will describe the specific embodiment of the invention in detail in conjunction with concrete accompanying drawing.It should be noted that the combination of technical characteristic or the technical characteristic described in following embodiment should not be considered to isolated, they can mutually be combined thus be reached better technique effect.
The invention provides a kind of method that Multiwalled Carbon Nanotubes Modified Electrode surveys resveratrol, comprise the following steps:
First glass-carbon electrode is polished with abrasive paper for metallograph, on sheet glass, mirror-smooth is polished to successively again with the alumina powder of 0.05 μm, all rinse with redistilled water after each polishing, then respectively at supersound washing 5min in absolute ethyl alcohol and redistilled water, obtain the glass-carbon electrode processed;
Pulverized by effective for single mortar, add concentrated hydrochloric acid ultrasonic disperse and remove wherein metallic catalyst, cleaning, to neutral, adds dense HNO
3, 140 DEG C of backflow 5h, after being cooled to room temperature, are washed till weakly acidic pH, after being placed in baking oven 120 DEG C of dryings, obtain multi-walled carbon nano-tubes;
Take above-mentioned multi-walled carbon nano-tubes 1mg and add 10ml DMF, ultrasonic 5 minutes, form black suspension;
Get black suspension described in 20 μ L, drop to the described glassy carbon electrode surface processed, dry under infrared lamp, obtain Multiwalled Carbon Nanotubes Modified Electrode;
Be working electrode with described Multiwalled Carbon Nanotubes Modified Electrode, revolving shape platinum electrode is to electrode, Ag/AgCl (3mol/L KCl) electrode is contrast electrode, get the Britton-Robinson buffer solution of pH2.0 in the electrolytic cell of 10.0mL, pass into nitrogen deoxygenation 10min, at 0 ~ 0.8V with cyclic voltammetry activated electrode until cyclic voltammetry curve stablize, then resveratrol liquid to be measured is added, speed is swept with 100mV/s under nitrogen protection condition, the cyclic voltammetry curve of record 0 ~ 0.8V, measure the oxidation peak current at-0.36V place, for the quantitative test of resveratrol.
Figure 1 shows that Multiwalled Carbon Nanotubes Modified Electrode of the present invention surveys the detection voltammogram of the method for resveratrol, measurement result as shown in Figure 1.
Multiwalled Carbon Nanotubes Modified Electrode of the present invention surveys the method for resveratrol, and method is simple, and Multiwalled Carbon Nanotubes Modified Electrode is easy to prepare, can repeatedly Reusability, and detection speed is fast, and accuracy rate is high.
Although give some embodiments of the present invention, it will be understood by those of skill in the art that without departing from the spirit of the invention herein, can change embodiment herein.Above-described embodiment is exemplary, should using embodiment herein as the restriction of interest field of the present invention.
Claims (1)
1. Multiwalled Carbon Nanotubes Modified Electrode surveys a method for resveratrol, it is characterized in that, comprises the following steps:
First glass-carbon electrode is polished with abrasive paper for metallograph, on sheet glass, mirror-smooth is polished to successively again with the alumina powder of 0.05 μm, all rinse with redistilled water after each polishing, then respectively at supersound washing 5min in absolute ethyl alcohol and redistilled water, obtain the glass-carbon electrode processed;
Pulverized by effective for single mortar, add concentrated hydrochloric acid ultrasonic disperse and remove wherein metallic catalyst, cleaning, to neutral, adds dense HNO
3, 140 DEG C of backflow 5h, after being cooled to room temperature, are washed till weakly acidic pH, after being placed in baking oven 120 DEG C of dryings, obtain multi-walled carbon nano-tubes;
Take above-mentioned multi-walled carbon nano-tubes 1mg and add 10ml DMF, ultrasonic 5 minutes, form black suspension;
Get black suspension described in 20 μ L, drop to the described glassy carbon electrode surface processed, dry under infrared lamp, obtain Multiwalled Carbon Nanotubes Modified Electrode;
Be working electrode with described Multiwalled Carbon Nanotubes Modified Electrode; revolving shape platinum electrode is to electrode; Ag/AgCl electrode is contrast electrode; get the Britton-Robinson buffer solution of pH2.0 in the electrolytic cell of 10.0mL; pass into nitrogen deoxygenation 10min; at 0 ~ 0.8V with cyclic voltammetry activated electrode until cyclic voltammetry curve stablize; then resveratrol liquid to be measured is added; speed is swept with 100mV/s under nitrogen protection condition; the cyclic voltammetry curve of record 0 ~-0.8V; measure the oxidation peak current at-0.36V place, for the quantitative test of resveratrol.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105572209A (en) * | 2015-12-23 | 2016-05-11 | 湖北民族学院 | Method utilizing liquid phase to strip graphene modified electrode to measure content of resveratrol |
| CN107144612A (en) * | 2017-04-20 | 2017-09-08 | 信阳师范学院 | Carbendazim detection method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080105544A1 (en) * | 2003-12-15 | 2008-05-08 | Fuji Xerox Co., Ltd. | Electrode for electrochemical measurement |
| CN101419186A (en) * | 2008-08-01 | 2009-04-29 | 南方医科大学 | Self-assembling electrode with resveratrol molecular imprinting and method for making same |
| WO2010004690A1 (en) * | 2008-07-09 | 2010-01-14 | 日本電気株式会社 | Carbon electrode, electrochemical sensor, and carbon electrode manufacturing method |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080105544A1 (en) * | 2003-12-15 | 2008-05-08 | Fuji Xerox Co., Ltd. | Electrode for electrochemical measurement |
| WO2010004690A1 (en) * | 2008-07-09 | 2010-01-14 | 日本電気株式会社 | Carbon electrode, electrochemical sensor, and carbon electrode manufacturing method |
| CN101419186A (en) * | 2008-08-01 | 2009-04-29 | 南方医科大学 | Self-assembling electrode with resveratrol molecular imprinting and method for making same |
Non-Patent Citations (1)
| Title |
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| WENSHENG HUANG 等: "Electrochemical Determination of Resveratrol Using Multi-Walled Carbon Nanotubes-Modified Glassy Carbon Electrode", 《NANOSCIENCE AND NANOTECHNOLOGY LETTERS》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105572209A (en) * | 2015-12-23 | 2016-05-11 | 湖北民族学院 | Method utilizing liquid phase to strip graphene modified electrode to measure content of resveratrol |
| CN105572209B (en) * | 2015-12-23 | 2018-04-03 | 湖北民族学院 | The method that graphene modified determination of electrode Resveratrol content is peeled off using liquid phase |
| CN107144612A (en) * | 2017-04-20 | 2017-09-08 | 信阳师范学院 | Carbendazim detection method |
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Application publication date: 20150819 |