The present invention is intended to excavate the exploitation Ilex latifolia resource again, and significantly improves the health care of bitter leaves tea.
The present invention is to be drinks such as the bag bubble compounded " kuding " tea that significant lipopenicillinase, step-down, fat-reducing and antiinflammation are arranged be processed into of raw material, compounded " kuding " tea beverage with bitter leaves tea (Ilex kudincha), gynostemma pentaphylla and excellent green tea.The processing method of bag bubble compounded " kuding " tea be with bitter leaves tea, gynostemma pentaphylla and excellent green tea raw material through removal of impurities, airing, complete, after oven dry and the pulverization process in 5~30: 3~30: 60~92 ratio is mixed packing, wherein bitter leaves tea children tender shoots leaf is earlier thick at draughty 20~35 ℃ indoor 3~5cm that is paved into, under 140~160 ℃ of temperature, complete after spreading 1~5 hour, spreading for cooling heat radiation rapidly, treat to knead when water-removing leaves is cooled to room temperature, be dried to water content with direct or indirect heating means at last and after 6%, pulverize again.The processing method of compounded " kuding " tea oral liquid is that three kinds of raw materials are crushed to below 16 orders through removal of impurities respectively, with flooding 2 times, behind the merging filtrate in 3~40: 3~40: 25~92 ratio mixing, sterilization, canned, wherein bitter leaves tea raw material is earlier with 5 times of boiling water immersions suction filtration after 2~3 hours, filter residue is used the constant temperature extracting about 30 minutes under 90~98 ℃ of conditions of 3 times of boiling water, merging filtrate behind the suction filtration again; The gynostemma pentaphylla powder soaked 1 hour with 5 times of boiling water, lixiviate suction filtration after 30 minutes under 99~100 ℃ of constant temperatures, and filter residue merges filtrate with 3 times of boiling water continuation constant temperature extractings under 99~100 ℃ of conditions twice about 1 hour again; Lixiviate below 25 ℃ 4~5 hours, tea grounds was used 3 times of boiling water again to the excellent green tea raw material behind the suction filtration with 4 times of cold water, continued lixiviate 30 minutes, suction filtration, merging filtrate under 90 ℃ of temperature.
Be described in further detail the present invention below.
Kudingcha is Aquifoliaceae Ilex (Ilex kudincha) plant, and aiphyllium is up to 20 meters, diameter of a cross-section of a tree trunk 1.3 meters above the ground 60cm, leaf are square circle or ovum shape square circle, long 4~30cm, wide 1.5~8cm, there is thin tooth at the edge, and the two sides does not have hair, above glossy, petiole tubbiness, long 1~2cm, dioecism spends majority to be arranged in false panicle, spherical drupe, about diameter 7mm, bronzing, exocarp are thick, 4 of pyrenes.
By analysis, contain cornutaside unit (Kudinchagenin), α-botany bar gum (α-angrin), cupreol (β-sitosterol), ursolic acid (Ursolic acid) and necessary multivitamin, amino acid and the trace element of human body in the bitter leaves tea shoot leaf.For the lipopenicillinase, step-down, fat-reducing and the antiinflammation that make bitter leaves tea-drinking product obvious, give full play to the drug effect of the active ingredient in the bitter leaves tea, in process, should strictly control process conditions, add man-hour as bag bubble bitter leaves tea, bitter leaves tea children tender shoots leaf is paved into 3~5cm is thick to be spread at draughty 20~35 ℃ indoor about 1~5 hour, with the continuous degree of being of the tender stem of record book in accordion form, surface moisture is distributed, change enzyme action direction in the bud-leaf, make the component ratio of nutriment and active ingredient suitable, keep the distinctive fragrance of bitter leaves tea simultaneously.And in the compounded " kuding " tea oral liquid process, when soaking with boiling water for the first time, stirred once every 15 minutes, under constant temperature during lixiviate, temperature can not surpass 98 ℃, and get final product about 30 minutes extracting time, should not be long for the second time.
Gynostemma pentaphylla is commonly called as the Radix Ginseng, the climing mouthfeel of not only improving bitter leaves tea in compounded " kuding " tea series beverage of pure sweet type gynostemma pentaphylla, and two kinds of plants have obtained augmenting to the health-care effect of human body, not only lowering blood-fat and reducing weight effect uniqueness, the also effect of tool anti-cancer.
Pharmacodynamic study through Hunan Province's traditional Chinese medicine research department shows that compounded " kuding " tea can reduce the TC of hyperlipidemia rats (serum cholesterol), TG (triglyceride), rising HOL (HDL) and reduce LOL (low-density lipoprotein) (seeing Table 1), and expression has effect for reducing blood fat; And can suppress the auricle edema (seeing Table 2) due to the mouse knoting oil, expression has antiinflammatory action.
Through the acute chronic toxicity test of this department, the compounded " kuding " tea maximal tolerance dose is 100g/kg again, is equivalent to 1666.7 times of clinical dosage (0.06g/kg), and prompting compounded " kuding " tea avirulence is safe in utilization.
32 routine hyperlipidemias and simple obesity clinical testing show:
(1) the lipopenicillinase total effective rate is 78.57%, and simple obesity fat-reducing total effective rate is 83%; (seeing Table 3),
(2) TC, TG reduce, HOL-CH raise (table 4);
(3) Secondary Obesity, simple obesity body weight descend and all reach the utmost point level of signifiance (P<0.001), and overweight person reaches the level of signifiance (P<0.05) (seeing Table 5);
(4) all case is before and after drinking, and routine urinalysis, routine blood test, platelet count, liver function, transaminase and electrocardiogram comparison check are all no abnormal.Remove indivedual cases in the drinking process and begin to occur having a stomach upset in 1-3 days, secret anguish, outside the spontaneous remission, all the other there is no toxicity thereafter, illustrate safe in utilization.
Compounded " kuding " tea series beverage both can be made beverage, quench the thirst and relieve summer heat, have again obvious health care effect, can prevent and to control diseases such as hypertension, artery sclerosis, coronary heart disease, abscess of throat, pharyngo-laryngitis chronicas, strong health, promote longevity, and hyperlipemia and simple obesity patient are had significant curative effect, and have no side effect, be a kind of desirable pure natural plant multifunctional health care drink.
Table 1 compounded " kuding " tea is to the influence of hyperlipidemia rats blood fat
(n=10,X±SD mol/l)
Group | Dosage (g/kg) | TG | TC | HDL | LDL |
Control group model group clofibrate group compounded " kuding " tea group compounded " kuding " tea group | 0.2 1.0 4.0 | 0.372±0.063※※※ 0.549±0.125 0.413±0.080※※※ 0.545±0.125※ 0.431±0.097※※ | 3.931±0.701※※※ 5.884±0.896 3.931±0.643※※※ 5.712±0.782※ 5.141±0.474※※ | 1.059±0.154※ 1.047±0.151 1.198±0.157※※ 1.159±0.081※ 1.300±0.172※※※ | 1.896±0.265※※ 2.761±0.259 1.750±0.239※※ 2.520±0.220※ 2.249±0.251※※ |
Compare ※ P>0.05, ※ ※ P<0.05, ※ ※ ※ P<0.01 (t check) with model group
Table 2 compounded " kuding " tea causes the influence of auricle edema to mouse knoting oil
(n=12,X±SD)
Group | Dosage (g/kg) | Swelling degree (mg) | Inhibiting rate (%) |
Control group aspirin group compounded " kuding " tea group compounded " kuding " tea group | 0.1 2.0 8.0 | 12.0±3.3 7.6±3.7※※※ 9.3±3.4※ 8.3±3.1※※※ | 36.7 22.5 30.8 |
Compare ※ P>0.05 ※ ※ ※ P<0.01 (t check) with control group
The lipopenicillinase of table 3 compounded " kuding " tea, curative effect on obesity
| Total routine number | Produce effects (example) | Effectively (example) | Invalid (example) | Total effective rate (%) |
Lipopenicillinase curative effect simple obesity curative effect | 28 (wherein 8 routine obesity) 12 | 19 3 | 3 7 | 6 2 | 78.57 83 |
Compare before and after the treatment of table 4 dyslipidemia index (X ± S)
| TC (mg/dl) 18 examples | TG (mg/dl) 24 examples | HOL-CH 18 examples |
Control back poor example (X) P value before controlling | 292.64±26.72 227.36±53.35 69.96±39.57 <0.001 | 282.06±134.27 238.15±167.59 130.36±95.58 <0.001 | 35.43±4.87 41.40±7.8 7.375±5.66 <0.001 |
Compare before and after the treatment of table 5 body weight abnormal index
| Simple obesity (kg) 12 examples | Secondary Obesity (kg) 9 examples | Overweight (10-<20%) is 7 examples (kg) |
Control back difference X P value before controlling | 74.42±10.09 70.79±9.17 3.63±2.24 <0.001 | 75 scholars 7.41 22.94 ± 6.98 2.06 ± 1.05<0.001 | 67.214±5.929 66.286±6.89 1.50±1.45 <0.05 |
Example one
(1) with the young tender shoots leaf of bitter leaves tea the well-ventilated, temperature is that 20-35 ℃ the indoor 3-5cm that is paved into is thick, spreads 1-5 hour, with the continuous degree of being of the tender stem of record book in accordion form, use special-purpose pot or green-keeping machine then, raise on the following stir-fry of 140-160 ℃ of condition of pot temperature limit, thoroughly the activity of destructive enzyme is with the leaf look dark green, tarnish, hand is pinched agglomerating, and loosing one's grip scatters is degree: the back spreading for cooling heat radiation rapidly that completes, when treating that water-removing leaves is reduced to room temperature, knead with hand or kneading machine, make cauline leaf cell partial destruction, leaf slivering rope deblocks while rubbing; Adopt directly (charcoal) or (heat pipe) mode of heating drying indirectly again, guarantee smokelessly to enter Ilex latifolia tea-leaf, dry back moisture after the removal of impurities, was shredded the 60-12 mesh sieve as raw material I once more below 6%;
(2) the bright gynostemma pentaphylla that will gather in the crops climing (pure sweet type), is cleaned removal of impurities, dries, be cut into 1cm long about, under 120-140 ℃ of temperature, complete, spreading for cooling is dried to room temperature, makes water content shred the 60-12 order after below 6% and makes raw material II;
(3) get free from admixture, pornographic movie is below 6%, and water content is made raw material II I less than 1% high mountain show eyebrow green tea;
(4) take by weighing 20% raw material I, 20% raw material II fully mixes to be divided in tea with in the filter paper bag with 60% raw material II I, and every bag 1.5 gram is a bag bubble bitter leaves tea, and the soup look emerald green, and bitter taste is laid particular stress on, a little less than the tea flavour.
Example two
The processing of three kinds of raw materials takes by weighing 5% raw material I with example one, and 3% raw material II is divided in tea with getting final product Instant Drinks in the filter paper bag after 92% raw material II I is fully mixed, and this product soup look green, and little hardship is time sweet, and tea flavour is dense.
Example three
(1) after the removal of impurities of bitter leaves tea early-products, be crushed to below 16 orders, the 5kg Broadleaf Holly Leaf adds 25kg boiling water and soaks 2-3 hour (stirring once every 15 minutes), suction filtration or centrifugal, filter residue adds boiling water 15kg, 98 ℃ of constant temperature extractings suction filtration or centrifugal after 30 minutes, and merging twice filtrate is raw material I;
(2) after the removal of impurities of gynostemma pentaphylla siccative, be crushed to below 16 orders, 5kg gynostemma pentaphylla powder adds boiling water 25kg and soaked 1 hour, 99-100 ℃ of (slow boil) extracting at constant temperature 30 minutes, suction filtration or centrifugal, filter residue add boiling water 15kg, 99-100 ℃ of (boiling) constant temperature extracting 1 hour, suction filtration or centrifugal, merging twice filtrate is raw material II;
(3) top grade green tea is raw material with the bright leaf of a little bud two, three leaves, be crushed to below 16 orders, 6.25kg adding cold water 25kg, soaked 4-5 hour green tea powder at low temperature below 25 ℃, stirred in 1 hour 1 time, suction filtration or centrifugal, tea grounds add water 18.75kg, and lixiviate is 30 minutes under 90 ℃ of temperature, suction filtration or centrifugal, two liquid are merged into raw material II I:
(4) get raw material I14.5kg, II14.5kg, III33.5kg, fully mixing, sterilization, can are the compounded " kuding " tea oral liquid, and the soup look green, and bitter partially time sweet.