CN104771778B - A kind of preparation method of micro/nano level Konjaku plucosidopolyose fiber scaffold material - Google Patents
A kind of preparation method of micro/nano level Konjaku plucosidopolyose fiber scaffold material Download PDFInfo
- Publication number
- CN104771778B CN104771778B CN201510135245.XA CN201510135245A CN104771778B CN 104771778 B CN104771778 B CN 104771778B CN 201510135245 A CN201510135245 A CN 201510135245A CN 104771778 B CN104771778 B CN 104771778B
- Authority
- CN
- China
- Prior art keywords
- fiber scaffold
- solution
- micro
- preparation
- nano level
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The present invention discloses the preparation method of a kind of micro/nano level Konjaku plucosidopolyose fiber scaffold material, belongs to biomedical material technical applications.The degradable biomaterial Konjaku plucosidopolyose that the present invention uses biocompatibility good is base stock, with alcohol/organic acid/aqueous systems as solvent, the Konjaku plucosidopolyose solution of preparation higher concentration, utilize electrostatic spinning technique, inhomogeneous field prepares micro/nano level Konjaku plucosidopolyose fibrous framework.The preparation method of this support has that preparation technology is brief, properties of product are stable, the more high advantage of productivity, through the micro/nano level Konjaku plucosidopolyose fibrous framework that the method is prepared, progenitor cells toxicity test result shows, when this support concentration is less than 5mg/ml, this support cytotoxicity standard is in 0 ~ 1 grade, there is no toxicity, illustrate that this support has potential bio-medical and is worth.
Description
Technical field:
The present invention relates to the preparation method of a kind of micro/nano level Konjaku plucosidopolyose fiber scaffold material, belong to bio-medical material technical applications.
Background technology:
In all processes preparing micro nanometer fiber material, electrostatic spinning is considered as the most also to be most efficient method.Due to micro nanometer fiber material simulation, natural extracellular matrix structure in tissue, is therefore with a wide range of applications in organizational project and regenerative medicine field.
Konjaku plucosidopolyose has multiple excellent specific property, or advantages of not being provided simultaneously with not available with natural macromolecular material including other synthesis such as biocompatibility, degradability, nothing or relatively low inflammatory reaction, good physical and mechanical properties, workability, abundance, extraction process are ripe simple.Therefore, its research in bio-medical field will be a new focus with application.
Though pertinent literature describes Rhizoma amorphophalli glucomannan fiber felt may have good application prospect in tissue engineering bracket field, such as the direction such as wound care dressings and medicament slow release.But prepare Konjaku plucosidopolyose fibrous framework with electrostatic spinning technique, yet suffer from many problems to be resolved, the hydrosol as the lowest in standard electrostatic spinning yield, nanofiber quality control, Konjaku plucosidopolyose unstable and with other synthesis or natural macromolecular material carry out being combined or be blended faced by problem etc..And the present invention is just for these problems, by selecting alcohol/organic acid/aqueous solvent, regulation proportions, spinning process selects inhomogeneous field, under suitable temperature field, has prepared micro/nano level Konjaku plucosidopolyose fiber scaffold material, and solving above all problems, the micro nanometer fiber support obtained is expected to be applied to previously described bio-medical field.
Summary of the invention
The problem to be solved in the present invention is to solve the problems such as existing Konjaku plucosidopolyose electrostatic spinning yields poorly, nanofiber quality control, the hydrosol are unstable.
It is an object of the invention to provide the preparation method of a kind of micro/nano level Konjaku plucosidopolyose fiber scaffold material, specifically include following steps:
(1) obtaining solution A after being mixed with acid/alcoholic solution by distilled water, in solution A, the volume fraction of distilled water is 75%, and the volume fraction of acid/alcoholic solution is 25%；
(2) adding Konjaku plucosidopolyose in solution A in the ratio of 20 ~ 45g/L, room temperature is thoroughly mixed and uniformly obtains solution B；
(3) solution B being filtered via 400 mesh sub-sieves, vacuum desiccator vacuum defoamation 6 ~ 12h obtains spinning liquid；
(4) take, with injector for medical purpose, the spinning liquid that step (3) obtains and complete electrostatic spinning process, select rustless steel syringe needle；
(5) vacuum room temperature drying sample, takes off and seals up for safekeeping, i.e. prepares uncrosslinked konjac glucomannan fiber scaffold A；
(6) under room temperature, standing crosslinking 12h ~ 24h in uncrosslinked konjac glucomannan fiber scaffold A is placed in crosslinked fluid, wherein, in crosslinked fluid, konjac glucomannan fiber scaffold A content is 0.5 ~ 1mg/ml；
(7) it is neutral for taking out the konjac glucomannan fiber scaffold distilled water in (6) and cleaning to cleanout fluid pH；
(8) at room temperature, vacuum is that under 400 ~ 500 millimetress of mercury, dry 24h, to being completely dried, takes out sealing preservation, i.e. prepares the konjac glucomannan fiber scaffold after cross-linking.
Acid/alcoholic solution of the present invention is glycerol/acetic acid, ethylene glycol/formic acid or propylene glycol/formic acid.
In acid/alcoholic solution of the present invention, acid solution and alcoholic solution volume fraction in solution A are 7% ~ 18%.
Electrostatic spray webbing process control environment temperature of the present invention is 30 ~ 50 DEG C, and (this temperature is unsuitable too high, high easily causing syringe needle blocking, spinning is discontinuous, the most unsuitable the most too low, easily there is strip in low syringe needle, spinning is in discontinuously), regulation positive voltage is 10 ~ 20Kv, and negative voltage is 0 ~ 4KV, receiving range is 10 ~ 20cm, the reception mode selected is non-homogeneous flat board (purpose is to manufacture inhomogeneous field, and this flat board can be needle plate, fluctuating flexure plane, band prominent hole flat board etc.), the external diameter of rustless steel syringe needle
0.07-1.04mm。
Crosslinked fluid of the present invention by ammonia and ethanol by volume ratio for 1:1 ~ 1:10 ammonia and ethanol mixing and stirring are obtained.
The principle of the present invention is: utilize organic acid, alcohol that Konjaku plucosidopolyose is modified improving the concentration of Konjaku plucosidopolyose solution, wherein alcohol be added to reduce spinning fluid viscosity and surface tension, but when glycerol content is too much, part konjak portuguese gansu polyose hydroxyl will be made preferentially to combine the little molecule of glycerol under Hyarogen-bonding, so that reducing the binding site of konjak portuguese gansu polyose glycan molecule and water, Rhizoma amorphophalli glucomannan dissolubility in water is reduced, so that there is lamination；And the addition of acid is precisely in order to suitably destroy this Hyarogen-bonding, thus improve the stability of solution, avoid the generation of lamination, additionally, due to the characteristic that Rhizoma amorphophalli glucomannan is sensitive to pH, the addition of acid can reduce the viscosity of this solution further, the hydrion that acid ionizes out can improve the electric conductivity of spinning liquid to a certain extent, and the two complements one another, thus solves spinnability problem in terms of solution parameter；Rough reception plate is possible not only to change electric field environment so that fibrous framework is prone to receive, additionally the spacing between salient point may also operate as being dried the effect of fiber felt, plus suitable ambient temperature, thus solve, from technological parameter, the problem that sample difficulty is collected, finally prepare the nano fiber scaffold that surface topography is good.
The present invention has at room temperature prepared the Konjaku plucosidopolyose of higher concentration with alcohol/organic acid/aqueous systems, by proportioning and the concentration of Konjaku plucosidopolyose of regulation alcohol/organic acid, micro/nano level Konjaku plucosidopolyose fiber scaffold material is prepared by electrostatic spinning technique, due to characteristics such as the good biocompatibilities that Konjaku plucosidopolyose itself has, so this material is expected to be used for biomedical sector.
The invention have the benefit that
(1) the micro/nano level Konjaku plucosidopolyose fiber scaffold material that the present invention prepares has that fibre diameter is little, the multiple specific function of bigger serface, high porosity, in biomedical sector, it is mainly used in organizational project, drug release, medical dressing, enzyme immobilizatio etc..
(2) in terms of medical angle, the micro/nano level Konjaku plucosidopolyose fiber scaffold material that the present invention prepares is structurally and functionally similar to natural extracellular matrix, and have preferable biocompatibility and certain intensity and structural stability, and degradable absorption, easy processing and fabricating again, and material can be regulated in many-sided performance such as physical chemistry, biology and mechanics, thus be expected to be used for preparing tissue engineering bracket.
(3) the method for the invention can regulate fibre diameter by the concentration of regulation Konjaku plucosidopolyose and spinning positive voltage, obtains the diversified support of different distributions form and pattern by changing reception device style.
(4) Konjaku plucosidopolyose can be effectively chemically crosslinked by alkali, when preparing the process of cross-linked kojacmannan fibrous framework, the crosslinking Konjaku plucosidopolyose fibrous framework of the different degree of cross linking can be obtained by the ratio of ethanol and ammonia in regulation crosslinked fluid, thus obtain mechanical strength, the fibrous framework that degradation rate differs.
(5) preparation method, low for equipment requirements, with low cost of this fiber scaffold material, by changing syringe needle quantity in spinning process, it is possible to achieve large-scale industrial production, thus meets the demand in the research of biomedical sector and clinical treatment.
Accompanying drawing explanation
Fig. 1 is the preparation technology flow chart of this fibrous framework；
Fig. 2 is the non-homogeneous reception flat board that this experiment is used；
Fig. 3 is the uncrosslinked fibrous framework pattern that obtains of embodiment 1 and diameter Distribution situation thereof；
Fig. 4 is the uncrosslinked fibrous framework pattern that obtains of embodiment 2 and diameter Distribution situation thereof；
Fig. 5 is the uncrosslinked fibrous framework pattern that obtains of embodiment 3 and diameter Distribution situation thereof；
Fig. 6 is fibrous framework pattern and the lixiviating solution cytotoxicity experiment result thereof of the crosslinking that embodiment 1 obtains.
Detailed description of the invention
With specific embodiment, the present invention is described in further detail below in conjunction with the accompanying drawings, but protection scope of the present invention is not limited to described content.
Embodiment 1
The preparation method of micro/nano level Konjaku plucosidopolyose fiber scaffold material described in the present embodiment, specifically includes following steps:
(1) measure 30ml distilled water with graduated cylinder to be placed in the beaker of 100ml, be sequentially added into 3ml glycerol and the stirring of 7ml glacial acetic acid makes it fully dissolve, obtain solution A；
(2) in solution A, add 0.8g Konjaku plucosidopolyose, stir (40min), obtain half thick shape solution B；
(3) solution B is filtered via 400 mesh sub-sieves, vacuum desiccator vacuum defoamation 6h；
(4) extracting 1 ~ 3ml solution with injector for medical purpose, select the rustless steel syringe needle in 0.07mm aperture, controlling ambient temperature is 30 DEG C, regulation positive voltage is 10Kv, and receiving range is 10cm, and the reception mode of selection is non-homogeneous flat board (needle plate, as shown in Figure 2 (a) shows), electrostatic spinning process is finally completed；
(5) it is 400 ~ 500 millimetress of mercury, drying at room temperature 24h sample in vacuum, takes off and seal up for safekeeping, i.e. prepare uncrosslinked konjac glucomannan fiber scaffold A；
(6), under room temperature, in uncrosslinked konjac glucomannan fiber scaffold A is placed in 20ml crosslinked fluid (ammonia and ethanol are by volume for the mixed mixed solution of ratio of 1:1), crosslinking 12h is stood；
(7) taking out the konjac glucomannan fiber scaffold distilled water in (6) and cleaning is neutral to cleanout fluid pH for several times；
(8) vacuum (400 millimetres of mercury), drying at room temperature 24h, takes out and seals preservation, i.e. prepares the konjac glucomannan fiber scaffold after crosslinking.
Uncrosslinked konjac glucomannan fiber scaffold A pattern that the present embodiment prepares and diameter Distribution situation thereof are as shown in Figure 3, the fibre diameter that the present embodiment prepares as seen from the figure is between 100 ~ 400nm scope, for micro/nano level, fibrous framework fibre diameter is more uniform, aperture is various, and this fibrous framework pattern is preferable.
Konjac glucomannan fiber scaffold pattern after the crosslinking that the present embodiment prepares and lixiviating solution cytotoxicity experiment result thereof are as shown in Figure 6, as seen from the figure: after alkali crosslinking Treatment, fibre diameter will broaden, and cytotoxicity experiment result shows, when this crosslinking support lixiviating solution concentration is less than 5.0mg/ml, this crosslinking support cell proliferation rate is higher than 80%, is 0 ~ 1 grade of toxicity level, support avirulence now is described, is expected to be used for tissue engineering bracket.
Embodiment 2
The preparation method of micro/nano level Konjaku plucosidopolyose fiber scaffold material described in the present embodiment, specifically includes following steps:
(1) measure 30ml distilled water with graduated cylinder to be placed in the beaker of 100ml, be sequentially added into 5ml ethylene glycol and the stirring of 5ml formic acid makes it fully dissolve, obtain solution A；
(2) in solution A, add 1.7g Konjaku plucosidopolyose, stir (40min), obtain half thick shape solution B；
(3) solution B is filtered via 400 mesh sub-sieves, vacuum desiccator vacuum defoamation 6h；
(4) 1 ~ 3ml solution is extracted with injector for medical purpose, select the rustless steel syringe needle in 0.5mm aperture, controlling ambient temperature is 40 DEG C, regulation positive voltage is 15 Kv, receiving range is 15cm, the reception mode selected is non-homogeneous flat board (wavy crooked metal sheet, as shown in Fig. 2 (b)), finally completes electrostatic spinning process；
(5) it is 400 ~ 500 millimetress of mercury, drying at room temperature 24h sample in vacuum, takes off and seal up for safekeeping, i.e. prepare uncrosslinked konjac glucomannan fiber scaffold A；
(6), under room temperature, in uncrosslinked konjac glucomannan fiber scaffold A is placed in 20ml crosslinked fluid (ammonia and ethanol are by volume for the mixed mixed solution of ratio of 1:6), crosslinking 18h is stood；
(7) it is neutral for taking out the konjac glucomannan fiber scaffold distilled water in (6) and cleaning to cleanout fluid pH；
(8) vacuum (450 millimetres of mercury), drying at room temperature 24h, takes out and seals preservation, i.e. prepares the konjac glucomannan fiber scaffold after crosslinking.
As shown in Figure 4, the fibre diameter that the present embodiment prepares as seen from the figure is between 100 ~ 450nm scope, for micro/nano level for uncrosslinked konjac glucomannan fiber scaffold A pattern that the present embodiment prepares and diameter Distribution situation thereof.
Embodiment 3
The preparation method of micro/nano level Konjaku plucosidopolyose fiber scaffold material described in the present embodiment, specifically includes following steps:
(1) measure 30ml distilled water with graduated cylinder to be placed in the beaker of 100ml, be sequentially added into 6ml propylene glycol and the stirring of 4ml formic acid makes it fully dissolve, obtain solution A；
(2) in solution A, add 1.8g Konjaku plucosidopolyose, stir (40min), obtain half thick shape solution B；
(3) solution B is filtered via 400 mesh sub-sieves, vacuum desiccator vacuum defoamation 6h；
(4) with the few 1 ~ 3ml of injector for medical purpose extraction, selecting the rustless steel syringe needle in 1.04mm aperture, controlling ambient temperature is 50 DEG C, regulation positive voltage is 20Kv, receiving range is 20cm, and the reception mode of selection is non-homogeneous flat board (needle plate), finally completes electrostatic spinning process；
(5) it is 400 ~ 500 millimetress of mercury, drying at room temperature 24h sample in vacuum, takes off and seal up for safekeeping, i.e. prepare uncrosslinked konjac glucomannan fiber scaffold A；
(6), under room temperature, in uncrosslinked konjac glucomannan fiber scaffold A is placed in 20ml crosslinked fluid (ammonia and ethanol are by volume for the mixed mixed solution of ratio of 1:10), crosslinking 12h is stood；
(7) taking out konjac glucomannan fiber scaffold A distilled water uncrosslinked in (6) and cleaning is neutral to cleanout fluid pH for several times；
(8) vacuum (500 millimetres of mercury), drying at room temperature 24h, takes out and seals preservation, i.e. prepares the konjac glucomannan fiber scaffold after crosslinking.
Uncrosslinked konjac glucomannan fiber scaffold A pattern and diameter Distribution situation thereof that the present embodiment prepares are as it is shown in figure 5, the fibre diameter that the present embodiment prepares as seen from the figure is between 50 ~ 500nm scope, for micro/nano level.
Claims (5)
1. the preparation method of a micro/nano level Konjaku plucosidopolyose fiber scaffold material, it is characterised in that specifically include following steps:
(1) obtaining solution A after being mixed with acid/alcoholic solution by distilled water, in solution A, the volume fraction of distilled water is 75%, and the volume fraction of acid/alcoholic solution is 25%；
(2) adding Konjaku plucosidopolyose in solution A in the ratio of 20 ~ 45g/L, room temperature is thoroughly mixed and uniformly obtains solution B；
(3) solution B being filtered via 400 mesh sub-sieves, vacuum desiccator vacuum defoamation 6 ~ 12h obtains spinning liquid；
(4) take, with injector for medical purpose, the spinning liquid that step (3) obtains and complete electrostatic spinning process, select rustless steel syringe needle；
(5) vacuum room temperature drying sample, takes off and seals up for safekeeping, i.e. prepares uncrosslinked konjac glucomannan fiber scaffold A；
(6) at room temperature, standing crosslinking 12h ~ 24h in uncrosslinked konjac glucomannan fiber scaffold A is placed in crosslinked fluid, wherein, in crosslinked fluid, konjac glucomannan fiber scaffold A content is 0.5 ~ 1mg/ml；
(7) the konjac glucomannan fiber scaffold distilled water cleaning in taking-up (6) is neutral to the pH of cleanout fluid；
(8) at room temperature, vacuum is that under 400 ~ 500 millimetress of mercury, dry 24h, to being completely dried, takes out sealing preservation, i.e. prepares the konjac glucomannan fiber scaffold after cross-linking.
The preparation method of micro/nano level Konjaku plucosidopolyose fiber scaffold material the most according to claim 1, it is characterised in that: described acid/alcoholic solution is glycerol/acetic acid, ethylene glycol/formic acid or propylene glycol/formic acid.
The preparation method of micro/nano level Konjaku plucosidopolyose fiber scaffold material the most according to claim 1, it is characterised in that: in described acid/alcoholic solution, acid solution and alcoholic solution volume fraction in solution A are 7% ~ 18%.
The preparation method of micro/nano level Konjaku plucosidopolyose fiber scaffold material the most according to claim 1, it is characterized in that: described electrostatic spray webbing process control environment temperature is 30 ~ 50 DEG C, regulation positive voltage is 10 ~ 20Kv, negative voltage is 0 ~ 4KV, receiving range is 10 ~ 20cm, the reception mode selected is non-homogeneous flat board, the external diameter 0.07-1.04mm of rustless steel syringe needle.
The preparation method of micro/nano level Konjaku plucosidopolyose fiber scaffold material the most according to claim 1, it is characterised in that: described crosslinked fluid by ammonia and ethanol by volume ratio for 1:1 ~ 1:10 ammonia and ethanol mixing and stirring are obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510135245.XA CN104771778B (en) | 2015-03-26 | 2015-03-26 | A kind of preparation method of micro/nano level Konjaku plucosidopolyose fiber scaffold material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510135245.XA CN104771778B (en) | 2015-03-26 | 2015-03-26 | A kind of preparation method of micro/nano level Konjaku plucosidopolyose fiber scaffold material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104771778A CN104771778A (en) | 2015-07-15 |
| CN104771778B true CN104771778B (en) | 2016-10-26 |
Family
ID=53613753
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510135245.XA Active CN104771778B (en) | 2015-03-26 | 2015-03-26 | A kind of preparation method of micro/nano level Konjaku plucosidopolyose fiber scaffold material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104771778B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105056301B (en) * | 2015-08-17 | 2018-02-09 | 昆明理工大学 | A kind of preparation method of annulus fibrosus disci intervertebralis tissue engineering bracket |
| CN106474556A (en) * | 2016-11-15 | 2017-03-08 | 昆明理工大学 | A kind of porous repair of cartilage timbering material and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101322857A (en) * | 2008-07-14 | 2008-12-17 | 昆明理工大学 | Compound osseous tissue engineering stephanoporate stent material and preparation thereof |
| CN102926016A (en) * | 2012-10-31 | 2013-02-13 | 西南科技大学 | Method for preparing modified konjac glucomannan fiber by electrostatic spinning |
| CN102926027A (en) * | 2012-10-31 | 2013-02-13 | 西南科技大学 | Method for preparing modified konjac glucomannan/biodegradation polyester polyblend fibers through electrostatic spinning |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6350463B1 (en) * | 1998-05-23 | 2002-02-26 | Andre Bieniarz | Method of treatment for premature rupture of membranes in pregnancy (PROM) |
| WO2007127795A2 (en) * | 2006-04-25 | 2007-11-08 | Bumgardner Joel D | Chitosan/nanocrystalline hydroxyapatite composite microsphere-based scaffolds |
-
2015
- 2015-03-26 CN CN201510135245.XA patent/CN104771778B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101322857A (en) * | 2008-07-14 | 2008-12-17 | 昆明理工大学 | Compound osseous tissue engineering stephanoporate stent material and preparation thereof |
| CN102926016A (en) * | 2012-10-31 | 2013-02-13 | 西南科技大学 | Method for preparing modified konjac glucomannan fiber by electrostatic spinning |
| CN102926027A (en) * | 2012-10-31 | 2013-02-13 | 西南科技大学 | Method for preparing modified konjac glucomannan/biodegradation polyester polyblend fibers through electrostatic spinning |
Non-Patent Citations (2)
| Title |
|---|
| HAP/CS/KGM复合骨组织工程支架的研究;洪东峰等;《武汉理工大学学报》;20100331;第32卷(第5期);第72-76页 * |
| 透明质酸钠/魔芋葡甘聚糖软骨支架材料的研究;朱向情等;《昆明理工大学学报（自然科学版）》;20120630;第37卷(第3期);第25-30页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104771778A (en) | 2015-07-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yao et al. | Macrofibers with high mechanical performance based on aligned bacterial cellulose nanofibers | |
| Ma et al. | Electrospun sodium alginate/poly (ethylene oxide) core–shell nanofibers scaffolds potential for tissue engineering applications | |
| Isogai et al. | TEMPO electromediated oxidation of some polysaccharides including regenerated cellulose fiber | |
| CN104511045B (en) | A kind of preparation method of the polyvinyl alcohol containing nano silver/chitosan nano fiber membrane dressing | |
| Wu et al. | Nanofibrous asymmetric membranes self-organized from chemically heterogeneous electrospun mats for skin tissue engineering | |
| CN104562292B (en) | Preparation method of porous micro-nano PET fibers | |
| CN104894856B (en) | Processing method for improving mechanical properties of silk fibroin nanofiber | |
| CN101270199B (en) | Method for improving hydrophilicity of polyacrylonitrile glycol cellulose nano-composite cellulosic membrane | |
| US9481963B2 (en) | Manufacturing method of carboxymethyl cellulose nonwoven fabric and use of carboxymethyl cellulose nonwoven fabric manufactured thereby | |
| CN103225126B (en) | Fibroin/sodium alginate composite nanofiber scaffold preparation method | |
| CN103436985A (en) | Preparation method of silk fibroin/chitosan blend nanofiber | |
| EP2080824A4 (en) | A process for producing fiber of ultra high molecular weight polyethylene | |
| CN101270198B (en) | Method for electrostatic blending polyacrylonitrile/glycol cellulose nano-fibrous membrane | |
| CN102277689A (en) | Device and method for preparing cellulose fibrous membrane with nanometer structure | |
| CN101922064A (en) | Bacterial cellulose and natural polysaccharide blend fiber and preparation method thereof | |
| CN103122583B (en) | Preparation method of novel amphiphilic nano fiber having core-shell structure | |
| CN105586716A (en) | High-content sodium alginate nanofiber membrane and electrostatic spinning manufacturing method thereof | |
| CN104771778B (en) | A kind of preparation method of micro/nano level Konjaku plucosidopolyose fiber scaffold material | |
| CN105536349A (en) | Novel nanometer aerogel material for air filtration and preparation method thereof | |
| CN104845382A (en) | Silk protein/cellulose derivative blending hydrogel and preparation method thereof | |
| Xin et al. | Carboxymethyl chitin/organic rectorite composites based nanofibrous mats and their cell compatibility | |
| CN110656380A (en) | Method for preparing different-form cellulose materials by electrostatic spinning based on ionic liquid | |
| CN102277654B (en) | Preparation method of hyaluronic acid and chitosan composite polyelectrolyte nanofibers | |
| Xuefei et al. | Poly (vinyl alcohol)–perfluorinated sulfonic acid nanofiber mats prepared via electrospinning as catalyst | |
| CN103865104A (en) | Preparation method of core-shell nano-particles |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20171108 Address after: 650051, 50 East Ring Road, Kunming, Yunnan (Kunming University of Science and Technology) Patentee after: Design and Research Institute of Kunming University of Science and Technology Address before: 650093 Kunming, Yunnan, Wuhua District Road, No. 253 Patentee before: Kunming University of Science and Technology |
|
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 650051 50 Huancheng East Road, Kunming City, Yunnan Province (inside Kunming University of Science and Technology) Patentee after: Kunming University of Technology Design and Research Institute Co.,Ltd. Address before: 650051 50 Huancheng East Road, Kunming City, Yunnan Province (inside Kunming University of Science and Technology) Patentee before: Design and Research Institute of Kunming University of Science and Technology |
|
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20150715 Assignee: YUNNAN YARONG MINING TECHNOLOGY CO.,LTD. Assignor: Kunming University of Technology Design and Research Institute Co.,Ltd. Contract record no.: X2023980041498 Denomination of invention: A preparation method of micro and nano scale konjac glucomannan fiber scaffold material Granted publication date: 20161026 License type: Common License Record date: 20230908 Application publication date: 20150715 Assignee: Yunnan Tangyuan Biotechnology Co.,Ltd. Assignor: Kunming University of Technology Design and Research Institute Co.,Ltd. Contract record no.: X2023980041492 Denomination of invention: A preparation method of micro and nano scale konjac glucomannan fiber scaffold material Granted publication date: 20161026 License type: Common License Record date: 20230908 Application publication date: 20150715 Assignee: Kunyu environment development (Yunnan) Co.,Ltd. Assignor: Kunming University of Technology Design and Research Institute Co.,Ltd. Contract record no.: X2023980041466 Denomination of invention: A preparation method of micro and nano scale konjac glucomannan fiber scaffold material Granted publication date: 20161026 License type: Common License Record date: 20230908 |