CN104614332B - Quantitative measurement method of cell DNA under redyeing environment - Google Patents
Quantitative measurement method of cell DNA under redyeing environment Download PDFInfo
- Publication number
- CN104614332B CN104614332B CN201510052983.8A CN201510052983A CN104614332B CN 104614332 B CN104614332 B CN 104614332B CN 201510052983 A CN201510052983 A CN 201510052983A CN 104614332 B CN104614332 B CN 104614332B
- Authority
- CN
- China
- Prior art keywords
- cell
- spectrum
- redyes
- cell dna
- under environment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention provides a quantitative measurement method of cell DNA under redyeing environment. The quantitative measurement method of the cell DNA under the redyeing environment comprises the steps: dyeing a slide carrying cast-off cells by using a plurality of same pure dyes; carrying out multispectral imaging on a multispectral imaging system which is not put into the slide to obtain a spectral image with incident intensity, then sequentially inserting slides which are dyed by pure dyes to calculate the absorbancy of the dyes; measuring absorbancy images of all the dyes; calculating to obtain a model as M=(XtX)-1Xt; and measuring an absorption spectrum image by using to-be-analyzed cells which are dyed by using the redyeing dyes and the multichannel imaging system, then calculating relative concentrations of various dyes on the cell according to the model M and the formula as c=My and calculating the relative content of DNA in the cell according to Feulgen dye concentration which is in direct ratio with the content of DNA in the cell. The method can be used for stripping Feulgen dyeing and Papanicolaou dyeing and can also be used for simultaneously carrying out quantitative measurement of DNA in the cell and quantitative analysis of cellular morphology.
Description
Technical field
The present invention relates to cast-off cells plate coating checking method, quantitative more particularly, to muti-spectrum imaging and cell component
Analysis method, belongs to cell dyeing technical field of imaging.
Background technology
Cervical carcinoma is one of gynaecology's malignant tumour occurred frequently, and the early diagnosis to cervical carcinoma and canceration is to improve cervical carcinoma to control
More the key of rate and survival rate.At present the most frequently used screening methods of cervical cancer of China is TBS classification, and early diagnosis of cancer needs
The cell of a few canceration is selected from up to ten thousand cells, this method needs doctor to carry out the artificial diagosis sieve of long-time
Look into, greatly increase the working strength and human error of doctor.
The diagnosis for carrying out precancerous lesions of uterine cervix with cell DNA image quantitative analysis diagnostic method is reported in a large number, in north
Beautiful and European cell DNA image quantitative analysis diagnostic method is as a kind of one of routine clinical detection method.At home, it is many
Big city also begins to carry out cervical carcinoma screening with full-automatic DNA quantified system analysises.Many studies have shown that, cell DNA quantitatively divides
Analysis diagnostic method has greater advantages in terms of early diagnosis.
There are two methods at present to combine TBS and DNA quantitative checks.
One kind is that cell smear is first done DNA dyeing, and with full-automatic DNA image quantified system analysis cervical carcinoma sieve is carried out
Look into, finding can obtain after cancer cell, DNA dyeing is faded again with chemical reagent, pap staining is then done again and is redyed, facilitate doctor
Directly check the cancer cell for checking.Have the disadvantage complex operation, the cancer cell for finding is possible to be come off during colour fading.
Another method is to do 2 cell smears, and a pap staining does TBS classification, a Fu Ergen (Feulgen)
The full-automatic DNA quantified system analysises of tint applications do DNA content measure, can once draw materials in clinical practice, while film-making, different
Dyeing, two kinds of technologies are detected simultaneously, achievable to have complementary advantages, and can significantly improve accuracy rate of diagnosis.Have the disadvantage DNA quantitative analyses system
The abnormal cell that system is found out, doctor cannot carry out TBS classification by familiar cellular morphology.Doctor carefully must also examine again
The slide of an other pap staining is looked into, search cancerous tumor cell is confirmed.
The content of the invention
The present invention proposes one kind and redyes cell DNA method for quantitative measuring under environment, can simultaneously carry out cell DNA quantitative
And morphometry；Simultaneously after the cancer cell that instrument DNA quantitative scannings are found out, doctor easily can be carried out in the original location
Cast-off cells TBS checks familiar to doctor.
The purpose of the present invention is by the following technical programs implementing：
One kind redyes cell DNA method for quantitative measuring under environment, comprises the following steps：
Step a, the slide for being loaded with cast-off cells is dyeed respectively with various single pure dyes；
Step b, the multi-optical spectrum imaging system to not being put into dyeing glass slide first carry out multispectral imaging, obtain incident intensity
Spectrum picture Io (λ), wherein λ be optical wavelength；
Step c, slide acquisition spectrum picture I (λ) for being sequentially inserted into pure dye dyeing again, calculate accordingly the extinction of dyestuff
Degree xi(λ)=log (Io (λ)/I (λ))；
Step d, repeat step c, measure the absorbance image x of all dyestuffsi(λ) it is, then comprehensive into sensitivity matrix
X, X=[x1, x2... ... xn],；
Step e, it is calculated model M=(XtX)-1Xt；
Step f, cell to be analyzed is redyed together with all dyestuffs in step a, will be loaded with the cell to be analyzed that contaminated
Slide is placed under microscope, and with multi-optical spectrum imaging system its absorption spectrum image y (λ) is measured, and is calculated according to formula c=My
The relative concentration of dyestuff, c=(c1,c2,c3,……cn), it is dyestuff concentration to be measured；Cell nuclear dna Jing diluted acids before dyeing
The aldehyde radical with reduction is produced after hydrolysis, the aldehyde radical is combined with Fu Ergen dyestuffs, and the concentration of Fu Ergen dyestuffs is proportional to
Nuclear DNA content, so as to calculate endonuclear DNA content according to the concentration of Fu Ergen dyestuffs.
The spectrum segment of the multi-optical spectrum imaging system is more than or equal to dyestuff number, to calculate the relatively dense of dyestuff
Degree.
The spectrum segment of the multi-optical spectrum imaging system adopts the spectrum segment of 480nm-680nm.
On the basis of the spectrum segment of setting, spectrum picture acquisition interval is 10-100nm.
The multi-optical spectrum imaging system, including object lens, imaging lens, video camera and computer, in the object lens and imaging lens
Between light path on electric tuning filter is installed.
The camera placements are in the rear end of imaging lens, and the imaging data of the video camera and the computer pair
Connect.
Further, the multi-optical spectrum imaging system also includes controller, to control the electric tuning filter in institute
State the position in the light path between object lens and the imaging lens.
The controller is docked with the computer, and receives the instruction of the computer, and then controls the electric tuning
The action of filter.
Feulgen's stain and pap staining can be peeled off with method proposed by the present invention.Cell DNA can simultaneously be carried out to determine
Amount and morphometry；The cancer cell found out after instrument DNA quantitative scannings simultaneously, doctor easily can enter in the original location
The raw familiar cast-off cells TBS that practises medicine is checked.
Description of the drawings
The present invention is described in further detail below according to drawings and Examples.
Fig. 1 be use dyestuff spectral curve, it can be seen that Yihong, BG, Fu Ergen dyestuffs visible light wave range suction
Receive spectrum and exist and overlap, therefore when redying, Yihong and BG can disturb the quantitative measurment of Fu Ergen dye strengths；
Fig. 2 is the multi-spectral imager structure chart that the present invention is adopted；
Fig. 3 is that we use liquid crystal tunable filter optical filtering curve；
Fig. 4 is to redye cell in 530nm wave band imaging effect figures, the light absorbs in main reflection Yihong；
Fig. 5 is to redye cell in 580nm wave band imaging effect figures, the light absorbs of main reflection Fu Ergen；
Fig. 6 is to redye cell in 50nm wave band imaging effect figures, the light absorbs of main reflection BG；
Fig. 7 is to redye cell using the design sketch that Fu Ergen dyestuffs are only remained after the stripping of this paper inventive methods, is had in cytoplasm
Slight residual is because that optical system still has dispersion, although we take the measure for reducing dispersion.This is from Fig. 4-6
It can be seen that coming, Fig. 5 is most clear, but Fig. 4 and Fig. 6 presence is fuzzy；
Fig. 8 be redye after using the cell DNA exponential distribution figure that measures of the present invention；
Fig. 9 is the same example cell DNA exponential distribution figure for only being measured with feulgen's stain.
Specific embodiment
As shown in figure 1, using dyestuff spectral curve, it can be seen that Yihong, BG, Fu Ergen dyestuffs are in visible light wave range
Absorption spectrum exist overlap.
It is as shown in Figure 2 the theory diagram of the muti-spectrum imaging instrument that we build.The white light that light source 1 sends illuminates load
Cast-off cells after dyeing on slide 2, cast-off cells are located in the focus of micro objective 3, therefore on cast-off cells
The light of point, becoming directional light after micro objective 3, then cell image is imaged on shooting by Jing microscope imagings mirror 6
On the target surface of machine 7, this is general microscopical operation principle.The system that we build is in micro objective 3 and microscope imaging
An electric tuning filter 4 (can be acousto-optic tunable filter AOTF and liquid crystal tunable filter) is inserted between mirror 6, is calculated
Machine 8 sends as needed control signal, and controls electric tuning filter 4 by needing by the controller 5 of electric tuning filter
The light of wave band is wanted, what is now collected on video camera is exactly the spectrum picture of the wave band.Adjust control electric tuning successively to filter
Device 4, obtains the micro- multispectral image of cell.
And electric tuning filter is placed on difference between microscope imaging mirror and CCD by general multi-optical spectrum imaging system, we
Electric tuning filter 4 is placed between micro objective 3 and microscope imaging mirror 6.It is known that when micro objective 3 and showing
When adopting parallel light path between micro mirror imaging lens 6, the distance between micro objective 3 and microscope imaging mirror 6 (light path) change
Have no effect on microscopical imaging.Electric tuning filter 4 is a thick optical body, has different dispersive powers to different wavelength,
Therefore just electric tuning filter is placed on different wave length focus difference when between microscope imaging mirror and CCD, it is difficult to while clear
Imaging, and our scheme hence helps to implement stripping algorithm proposed by the present invention without this problem.
First, the smear of cast-off cells is dyeed respectively with the single pure dye redyed.Start multi-optical spectrum imaging system, it is right
Not being put into the system of dyeing glass slide first carries out multispectral imaging, obtains incident intensity spectrum picture Io (λ), and wherein λ is optical wavelength；
Then the slide for being sequentially inserted into pure dye dyeing again obtains spectrum I (λ), and absorbance x of dyestuff can be calculated accordinglyi(λ)=
log(Io(λ)/I(λ))；Measure the absorbance image x of all dyestuffsi(λ) (I=1-N, N are the sum of dyestuff), then comprehensive
Matrix X in composite formula (3).Then model M=(X is calculatedtX)-1Xt.(X represents matrix, XtRepresent the matrix transposition,
X-1Represent the matrix inversion)
Hereafter, cell to be analyzed can be redyed with all dyestuffs, the slide for being loaded with the cell to be analyzed for having contaminated is placed in aobvious
Under micro mirror, its absorbance image y (λ) is measured with multi-optical spectrum imaging system, then calculate various dyestuffs according to formula (4)
Relative concentration, by the concentration of Fu Ergen dyestuffs the DNA content of cell can be then calculated.
As long as the spectrum segment of multispectral imaging is more than or equal to dyestuff number, the relative concentration of dyestuff just can be calculated,
But the appropriate number for increasing spectrum segment can improve the precision of measurement.
Less in view of short-wave band light signal strength, noise is big, and the absorption of each dyestuff is less after 700nm.I
Employ the spectrum segment of 480nm-680nm, a spectrum picture is gathered per 10nm.
Fig. 3 is redyed using dyestuff spectral curve, and model M=(X is calculated accordinglytX)-1Xt。
Fig. 4-6 is to redye cell 530,580 and 650nm wave band imaging effect figures, it can be seen that in three wave bands, is owned
Dyestuff has absorption, and overlaps；
Fig. 7 is to redye cell using the design sketch that Fu Ergen dyestuffs are only remained after the stripping of this paper inventive methods, it can be seen that only
Having on nucleus has absorption, and it is the absorption of Fu Ergen dyestuffs；
Fig. 8 be redye after using the cell DNA exponential distribution histogram that measures of the present invention；
Fig. 9 is the same example cell DNA exponential distribution histogram for only being measured with feulgen's stain；It can be seen that using this
The bright cell DNA index measured when redying and same example cell only measure the basic phase of DNA exponential distribution figures with feulgen's stain
Together, illustrate to adopt the inventive method, Yihong and BG can be well deducted when redying on DNA measure spectrum sections (580nm)
Impact.
DNA quantitative cytology mainly judged by the measure of DNA content in nucleus the physiological status of cell and
Pathological change.Fu Ergen DNA dyeing be a kind of energy it is single-minded show DNA colouring method, the face after nuclear targeting
Color depth is shallow relevant with DNA content.According to Lambert-Beer laws, monochromatic absorbance is proportional to the content of material, material
Content it is more, absorb light it is more, the transmission of light is lower, and Lambert-Beer law expression formulas are：
A=-lg (I/I0)=ε bC (1)
In formula, I0For parallel, the uniform incident homogeneous beam intensity that wavelength is λ；I is through the beam intensity after target；A
For luminosity degree；ε is the molar absorption coefficient of component to be measured；B is light path；C is the substance withdrawl syndrome of component to be measured.
The quality of human normal diploid cell DNA is about 7.18pg, using Lambert-Beer laws and image procossing
Method measure the integral optical density of whole cell, and can be with by the ratio of integral optical density and normal cell integral optical density
Obtain the DNA content of cell to be measured.
TBS classification uses the bus dyeing that the dyestuffs such as Yihong, BG, haematoxylin carry out, feature be nucleus and
Cytoplasm form shows clearly there is abundant color information.But we have observed that from accompanying drawing 1, Yihong, the absorption spectrum of BG and good fortune
You have overlap by the absorption spectrum of root dyestuff.Traditional DNA quantitative analyses use the monochromatic light of a specific band (generally
580nm), in this spectrum segment, the absorption of Yihong, BG is while be superimposed upon in the absorption optical density measurements of Fu Ergen when redying
Face, therefore cannot correct measurement cell DNA content.
Using multispectral imaging, then can there is favourable turn in problem.Multispectral imaging is a kind of new imaging technique, is obtained
It is a three-dimensional data, for the image that specific wave band can obtain cell, for any point of cell then can be obtained
One absorption spectrum.
According to spectral theory, the absorption spectrum y (λ) of any point can use following linear adduction Mathematical Modeling table on cell
Illustrate:
Y=c1x1+c2x2+…+cnxn+e (2)
In formula：Y represents the spectrum vector for redying dyestuff, xi(i=1,2 ... n) for single pure dye spectrum, it is noted that
Different materials should have different spectrum vectors, so xi(i=1,2 ... n) be mutual linear independence vector；E is error
Vector, e=[e1,e2,……en], generally Normal Distribution etc. variance white noise error；N be dyestuff kind number, c=
(c1,c2,c3,……cn) it is unknown parameter to be measured, if being the relative concentration of dyestuff. represented using matrix, above formula can be rewritten
For:
Y=Xc+e (3)
In formula:X=[x1, x2... ... xn], can be described as sensitivity matrix, c=(c1,c2,…,cn) it is unknown parameter to be estimated
Vector, or concentration vector referred to as to be measured.The curve of spectrum of dyestuff is as shown in Figure 1：
To make to be estimated survey vector and actual measurement vector error minimum, that is, causeTend to most
It is little, order
In noticing above formula, only c is unknown quantity, therefore can obtain the minimum of f (c) to its derivation.Because
In the derivation of above formula, becauseFor a scalar, therefore haveTherefore above formula derivation can be obtained：
Make above formula be equal to zero, can obtain immediately,
XtXc=Xty
That is,
Make M=(XtX)-1Xt
Then c=My
So far, above formula shows：Model M is set up by the light spectrum matrix X of pure dye, from the compound suction that multispectral imaging is measured
Receive the relative concentration that spectrum y calculates various dyestuffs.
Claims (10)
1. one kind redyes cell DNA method for quantitative measuring under environment, it is characterised in that the method is comprised the following steps：
Step a, the slide for being loaded with cast-off cells is dyeed respectively with various single pure dyes；
Step b, the multi-optical spectrum imaging system to not being put into dyeing glass slide first carry out multispectral imaging, obtain the light of incident intensity
As Io (λ), wherein λ is optical wavelength to spectrogram；
Step c, slide acquisition spectrum picture I (λ) for being sequentially inserted into pure dye dyeing again, calculate accordingly absorbance x of dyestuffi
(λ)=log (Io (λ)/I (λ))；
Step d, repeat step c, measure the absorbance image x of all dyestuffsi(λ) it is, then comprehensive into sensitivity matrix X, X=
[x1, x2... ... xn]；Wherein, i=1-n, n are the sum of dyestuff；
Step e, it is calculated model M=(XtX)-1Xt；Wherein, X represents matrix, XtRepresent the matrix transposition, X-1Representative should
Matrix inversion；
Step f, cell to be analyzed is redyed together with all dyestuffs in step a, will be loaded with the slide of the cell to be analyzed for having contaminated
Under being placed in microscope, with multi-optical spectrum imaging system its absorption spectrum image y (λ), y=[y are measured1,y2,……yn], Ran Houyi
The relative concentration of dyestuff, c=(c are calculated according to formula c=My1,c2,c3,……cn), it is dyestuff concentration to be measured；
Cell nuclear dna produces the aldehyde radical with reduction, the aldehyde radical and Fu Ergen dyestuffs Jing after dilute acid hydrolysis before dyeing
With reference to the concentration of Fu Ergen dyestuffs is proportional to nuclear DNA content, so as to be calculated carefully according to the concentration of Fu Ergen dyestuffs
DNA content in karyon.
2. one kind as claimed in claim 1 redyes cell DNA method for quantitative measuring under environment, it is characterised in that the light more
The spectrum segment of spectrum imaging system is more than or equal to dyestuff number, to calculate the relative concentration of dyestuff.
3. one kind as claimed in claim 2 redyes cell DNA method for quantitative measuring under environment, it is characterised in that the light more
The spectrum segment of spectrum imaging system adopts the spectrum segment of 480nm-680nm.
4. one kind as claimed in claim 3 redyes cell DNA method for quantitative measuring under environment, it is characterised in that in setting
On the basis of spectrum segment, spectrum picture acquisition interval is 10-100nm.
5. the one kind as described in any one of Claims 1-4 redyes cell DNA method for quantitative measuring under environment, it is characterised in that
The multi-optical spectrum imaging system, including object lens, imaging lens, video camera and computer, the light between the object lens and imaging lens
Electric tuning filter is installed on road.
6. one kind as claimed in claim 5 redyes cell DNA method for quantitative measuring under environment, it is characterised in that the shooting
Machine is placed in the rear end of imaging lens, and the imaging data of the video camera is docked with the computer.
7. one kind as claimed in claim 6 redyes cell DNA method for quantitative measuring under environment, it is characterised in that also including control
Device processed, to control the position in light path of the electric tuning filter between the object lens and the imaging lens.
8. one kind as claimed in claim 7 redyes cell DNA method for quantitative measuring under environment, it is characterised in that the control
Device is docked with the computer, and receives the instruction of the computer, and then controls the action of the electric tuning filter.
9., such as Claims 1-4, the one kind described in 6 to 8 any one redyes cell DNA method for quantitative measuring under environment, its feature
Be, step a, step b sequencing it is adjustable.
10. such as Claims 1-4, the one kind described in 6 to 8 any one redyes cell DNA method for quantitative measuring under environment, and it is special
Levy and be, also include the step of peeling off feulgen's stain and pap staining using methods described.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510052983.8A CN104614332B (en) | 2015-02-02 | 2015-02-02 | Quantitative measurement method of cell DNA under redyeing environment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510052983.8A CN104614332B (en) | 2015-02-02 | 2015-02-02 | Quantitative measurement method of cell DNA under redyeing environment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104614332A CN104614332A (en) | 2015-05-13 |
| CN104614332B true CN104614332B (en) | 2017-05-10 |
Family
ID=53148877
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510052983.8A Active CN104614332B (en) | 2015-02-02 | 2015-02-02 | Quantitative measurement method of cell DNA under redyeing environment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104614332B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020041781A1 (en) * | 2018-08-23 | 2020-02-27 | Essenlix Corporation | Enhanced, rapid, homogeneous, cell staining and assay |
| CN109540890A (en) * | 2018-11-27 | 2019-03-29 | 湖南品胜生物技术有限公司 | A kind of DNA quantitative analysis method based on microcytoscope image |
| CN110895963A (en) * | 2019-10-31 | 2020-03-20 | 深圳兰丁医学检验实验室 | Cell DNA quantitative determination system based on artificial intelligence |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1553165A (en) * | 2003-12-19 | 2004-12-08 | 武汉大学 | Automatic multispectral cell smear and analyzing instrument and method used for cervical cell analysis |
| CN101339185A (en) * | 2008-06-23 | 2009-01-07 | 武汉呵尔医疗科技发展有限公司 | Automatic microscopic imager for detecting cast-off cells and detection method |
| CN101560544A (en) * | 2008-04-18 | 2009-10-21 | 麦克奥迪实业集团有限公司 | Method for integrative detection of cells |
| CN201788111U (en) * | 2010-09-19 | 2011-04-06 | 王珑 | All-in-one machine for fast slide-making and staining of liquid-based thinlayer cells and cell DNAs |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006220522A (en) * | 2005-02-10 | 2006-08-24 | National Institute For Materials Science | Determination method of bonding mode between dna and dna bonding molecule and manufacturing method of sample used for it |
-
2015
- 2015-02-02 CN CN201510052983.8A patent/CN104614332B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1553165A (en) * | 2003-12-19 | 2004-12-08 | 武汉大学 | Automatic multispectral cell smear and analyzing instrument and method used for cervical cell analysis |
| CN101560544A (en) * | 2008-04-18 | 2009-10-21 | 麦克奥迪实业集团有限公司 | Method for integrative detection of cells |
| CN101339185A (en) * | 2008-06-23 | 2009-01-07 | 武汉呵尔医疗科技发展有限公司 | Automatic microscopic imager for detecting cast-off cells and detection method |
| CN201788111U (en) * | 2010-09-19 | 2011-04-06 | 王珑 | All-in-one machine for fast slide-making and staining of liquid-based thinlayer cells and cell DNAs |
Non-Patent Citations (2)
| Title |
|---|
| 基于图像分析的DNA定量分析研究;汪天仪等;《时珍国医国药》;20061130;第17卷(第11期);第2325-2327页 * |
| 多光谱成像系统在病理诊断中的应用;高俊等;《临床与实验病理学杂志》;20111231;第27卷(第12期);第1347-1349页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104614332A (en) | 2015-05-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2301706T3 (en) | METHOD OF QUANTITATIVE VIDEOMICROSCOPY AND ASSOCIATED SYSTEM AS WELL AS THE SOFWARE INFORMATION PROGRAM PRODUCT. | |
| US9002077B2 (en) | Visualization of stained samples | |
| US9779503B2 (en) | Methods for measuring the efficacy of a stain/tissue combination for histological tissue image data | |
| US8816279B2 (en) | Tunable laser-based infrared imaging system and method of use thereof | |
| US20030207250A1 (en) | Methods of diagnosing disease | |
| JP2002530676A (en) | Cell in situ analysis method | |
| AU2014293317A1 (en) | Optical detection of skin disease | |
| CN104614332B (en) | Quantitative measurement method of cell DNA under redyeing environment | |
| CN103630091B (en) | Leaf area measurement method based on laser and image processing techniques | |
| US8189884B2 (en) | Methods for assessing molecular expression of subcellular molecules | |
| CN101750272A (en) | Blood cell image recognition counting method | |
| JP2002521682A (en) | Cell in situ analysis method | |
| JP2002521682A5 (en) | ||
| Zhou et al. | A multiple wavelength algorithm in color image analysis and its applications in stain decomposition in microscopy images | |
| CN103837461B (en) | A kind of gray scale photographic head and there is the cell comprehensive analysis device of high efficiency illumination | |
| JP2009014355A (en) | Image processor and processing program | |
| CN110288711A (en) | The detection method of bituminous pavement three-D grain pattern | |
| JP2022023916A (en) | Device and method for detecting pulse and information processing program | |
| CN105866035A (en) | Rapid non-destructive tissue biopsy method and technique based on spatial frequency domain-modulated large area resolution microstructure | |
| Septiana et al. | Elastic and collagen fibers discriminant analysis using H&E stained hyperspectral images | |
| JP6305623B2 (en) | Optical composition analysis of mixtures | |
| CN113936005A (en) | DNA index calculation method and device, computer equipment and storage medium | |
| CN107690575A (en) | The infrared imaging of biomaterial | |
| Di Cecilia et al. | Design and performance of a hyperspectral imaging system: Preliminary in vivo spectral reflectance measurements of the human iris | |
| JPWO2018131091A1 (en) | Image processing apparatus, image processing method, and image processing program |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |