CN104489526A - 一种保健纳豆的制作方法 - Google Patents
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Abstract
一种保健纳豆的制作方法涉及的是一种豆制品的制备方法，具体地说是一种保健纳豆的制作方法。称取一定质量的黄豆，加水浸泡后捞出沥干水分，加入富硒酵母和亚硒酸钠搅拌均匀，放到高压锅中121℃蒸40-50分钟，冷却到30℃，接入纳豆芽孢杆菌种子液，迅速拌匀，分装到塑料盒中，放到人工气候箱中，在温度30℃、相对湿度70-90%的条件下培养20～24小时，取出放入4℃冰箱中后熟24小时即得成品。用本发明的方法制作的纳豆中硒的含量远远超过市售纳豆中硒的含量。
Description
技术领域
本发明涉及的是一种豆制品的制备方法，具体地说是一种保健纳豆的制作方法。
背景技术
纳豆是以黄豆为原料，接种纳豆芽孢杆菌后经过发酵制作而成。纳豆的保健功能主要与其中的纳豆激酶、皂青素、维生素K2等多种功能因子有关。纳豆中的纳豆激酶是一种丝氨酸蛋白酶，它具有溶解血栓，降低血粘度，改善血液循环，软化和增加血管弹性等作用，因此纳豆已成为当今治疗和预防血栓的一颗新星；纳豆中的皂青素可以改善便秘，降低血脂，预防肠癌、降低胆固醇、软化血管、预防高血压和动脉硬化，抑制艾滋病病毒；维生素K2能生成骨蛋白质，骨蛋白质再与钙共同生成骨质，增加骨密度，防止骨折，所以维生素K2可以治疗和预防骨质疏松症。
硒是联合国卫生组织继碘、锌之后,明确确定的人体所必须的重要微量元素，硒在地球表面分布很不平均。大部分土壤缺硒，造成农产品缺硒，引起人体缺硒，造成心脑血管系统疾病和癌症病人增多。硒能提高人体免疫，促进淋巴细胞的增殖及抗体和免疫球蛋白的合成，硒对结肠癌、皮肤癌、肝癌、乳腺癌等多种癌症具有明显的抑制和防护的作用，同时，硒具有减轻和缓解重金属毒性的作用。目前，市售的富硒食品主要是在动、植物的自然生长过程中，将硒元素添加到动物饲料中或者导入植物体内，从而生产有机硒含量较高的食品，例如富硒牛奶、富硒大豆等。由于微生物对硒元素的富集效率和富集能力要远远超过动、植物，所以许多微生物领域的科研人员进行了大量的利用微生物富集硒的研究，目前已经开发出富硒酵母等微生物富硒产品。
传统的富硒纳豆都是利用富硒黄豆发酵制成的。纳豆芽孢杆菌对硒有较强的富集能力，用普通黄豆作原料生产纳豆，在发酵过程中添加硒，可以生产出富含有机硒的纳豆，这种利用微生物对硒的富集的效率要远远高于植物富硒的效率。微生物富集的硒与动植物富集的硒一样都属于有机硒，人体补充有机硒与补充无机硒相比，有机硒可以消除无机硒对人体的毒副作用和对胃肠的刺激,能被人体更高效,更安全地吸收利用。
发明内容
本发明的目的在于提供一种富含有机硒的保健纳豆的制作方法。
本发明的目的是这样实现的：
1、纳豆芽孢杆菌种子液的制备
称取3g牛肉膏、10g蛋白胨、5g氯化钠、50g葡萄糖、10mg亚硒酸钠，加水溶解并定容至1000ml，调节PH至7.0，即得纳豆芽孢杆菌种子液培养基。将种子液培养基在121℃条件下灭菌20分钟，冷却至30℃，接种纳豆芽孢杆菌，在28～32℃、旋转摇床200rpm的条件下培养15～18小时。
2、保健纳豆的制作
称取一定质量的黄豆，加水浸泡14—18小时，浸泡结束后捞出沥干水分，按照每1000克干黄豆加入0.5克富硒酵母的比例加入富硒酵母，再按照每1000克干黄豆加入5毫克亚硒酸钠的比例加入亚硒酸钠，搅拌均匀，放到高压锅中121℃蒸40-50分钟，冷却到30℃，按照干黄豆质量的2%的比例接入纳豆芽孢杆菌种子液，迅速拌匀，分装到塑料盒中，厚度6～8厘米，在每个塑料盒上盖上6层灭菌纱布，放到人工气候箱中，在温度30℃、相对湿度70-90%的条件下培养20～24小时，取出放入4℃冰箱中后熟24小时即得成品。
分别测定用本发明的方法制作的纳豆和市售的纳豆中硒的含量和纳豆激酶的酶活力，测定结果见下表：
| 市售纳豆 | 本发明纳豆 | |
| 硒含量（μg/kg） | 30.82 | 1124.35 |
| 纳豆激酶酶活力（IU/g） | 477.15 | 489.48 |
从上表可以看出，用本发明的方法制作的纳豆，其纳豆激酶活力与市售纳豆大致相当，说明在发酵过程中添加的少量硒没有抑制纳豆芽孢杆菌的代谢。而用本发明的方法制作的纳豆中硒的含量远远超过市售纳豆中硒的含量。
具体实施方案
本发明采用的菌株采购于中国工业微生物菌种保藏管理中心，为纳豆芽孢杆菌CICC20640。
称取3g牛肉膏、10g蛋白胨、5g氯化钠、50g葡萄糖、亚硒酸钠10mg，加水溶解并定容至1000ml，调节PH至7.0，即得纳豆芽孢杆菌种子液培养基。将种子液培养基在121℃条件下灭菌20分钟，冷却至30℃，接种纳豆芽孢杆菌，在28～32℃、旋转摇床200rpm的条件下培养15～18小时。
称1000g黄豆，加水浸泡16小时，浸泡结束后捞出沥干水分，加入0.5克富硒酵母、5毫克亚硒酸钠，搅拌均匀，放到高压锅中121℃蒸45分钟，冷却到30℃，接入20克纳豆芽孢杆菌种子液，迅速拌匀，分装到塑料盒中，厚度8厘米，在每个塑料盒上盖上6层灭菌纱布，放到人工气候箱中，在温度30℃、相对湿度80%的条件下培养22小时，取出放入4℃冰箱中后熟24小时即得成品。
Claims (1)
1.一种保健纳豆的制作方法，其特征是经过如下加工过程制备而成：
⑴称取3g牛肉膏、10g蛋白胨、5g氯化钠、50g葡萄糖、10mg亚硒酸钠，加水溶解并定容至1000ml，调节PH至7.0，即得纳豆芽孢杆菌种子液培养基，将种子液培养基在121℃条件下灭菌20分钟，冷却至30℃，接种纳豆芽孢杆菌，在28～32℃、旋转摇床200rpm的条件下培养15～18小时即得纳豆芽孢杆菌种子液；
⑵称取一定质量的黄豆，加水浸泡14—18小时，浸泡结束后捞出沥干水分，按照每1000克干黄豆加入0.5克富硒酵母的比例加入富硒酵母，再按照每1000克干黄豆加入5毫克亚硒酸钠的比例加入亚硒酸钠，搅拌均匀，放到高压锅中121℃蒸40-50分钟，冷却到30℃，按照干黄豆质量的2%的比例接入纳豆芽孢杆菌种子液，迅速拌匀，分装到塑料盒中，厚度6～8厘米，在每个塑料盒上盖上6层灭菌纱布，放到人工气候箱中，在温度30℃、相对湿度70-90%的条件下培养20～24小时，取出放入4℃冰箱中后熟24小时即得成品。
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106490483A (zh) * | 2016-10-20 | 2017-03-15 | 山东健康源生物工程有限公司 | 一种含富硒酵母和纳豆冻干粉胶囊剂的制备工艺 |
| CN107897719A (zh) * | 2017-12-01 | 2018-04-13 | 凯风新农（北京）科技有限公司 | 一种纳豆复合制剂及其制备方法 |
| CN110075282A (zh) * | 2019-05-20 | 2019-08-02 | 湖南康琪壹佰生物科技有限公司 | 一种缓解血栓的纳豆复合物及其制备方法 |
| CN112870252A (zh) * | 2021-03-22 | 2021-06-01 | 天津市宝恒生物科技有限公司 | 一种具有抗癌作用的生物发酵组合物及其制备方法和应用 |
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| CN114984065A (zh) * | 2022-06-08 | 2022-09-02 | 余昭军 | 一种提高免疫力的益生菌组合物及其制备方法 |
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| CN106490483A (zh) * | 2016-10-20 | 2017-03-15 | 山东健康源生物工程有限公司 | 一种含富硒酵母和纳豆冻干粉胶囊剂的制备工艺 |
| CN107897719A (zh) * | 2017-12-01 | 2018-04-13 | 凯风新农（北京）科技有限公司 | 一种纳豆复合制剂及其制备方法 |
| CN110075282A (zh) * | 2019-05-20 | 2019-08-02 | 湖南康琪壹佰生物科技有限公司 | 一种缓解血栓的纳豆复合物及其制备方法 |
| CN112870252A (zh) * | 2021-03-22 | 2021-06-01 | 天津市宝恒生物科技有限公司 | 一种具有抗癌作用的生物发酵组合物及其制备方法和应用 |
| CN113040333A (zh) * | 2021-04-30 | 2021-06-29 | 上海麦玺隆机械科技有限公司 | 一种生产有机硒富硒豆类制品的方法 |
| CN114984065A (zh) * | 2022-06-08 | 2022-09-02 | 余昭军 | 一种提高免疫力的益生菌组合物及其制备方法 |
| CN114984065B (zh) * | 2022-06-08 | 2023-07-21 | 宝健（北京）生物技术有限公司 | 一种提高免疫力的益生菌组合物及其制备方法 |
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