CN104488986A - 一种检验科用中药杀菌液 - Google Patents
一种检验科用中药杀菌液 Download PDFInfo
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- CN104488986A CN104488986A CN201410822803.5A CN201410822803A CN104488986A CN 104488986 A CN104488986 A CN 104488986A CN 201410822803 A CN201410822803 A CN 201410822803A CN 104488986 A CN104488986 A CN 104488986A
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Abstract
本发明涉及中药应用领域，特别公开了一种检验科用中药杀菌液。该一种检验科用中药杀菌液，由金银花，鱼腥草，黄连，板蓝根，柴胡，黄芩，花椒份，连翘，皂角，菊花按照一定的重量配比采用现代先进的医药制药技术制成，具有杀菌能力强，作用速度快，稳定性好的显著优点，适应于检验科以及医院其他科室的杀菌消毒，不污染环境，对人和动物无刺激，无毒副作用，是一种值得推广的理想杀菌药物。
Description
(一)技术领域
本发明涉及中药应用领域，特别涉及一种检验科用中药杀菌液。
(二)背景技术
检验科是临床医学和基础医学之间的桥梁，包括临床化学、临床微生物学、临床免疫学、血液学、体液学以及输血学等分支学科。检验科作为医院进行医学检验的科室，检验工作需要在无菌条件下进行，以防止细菌、真菌以及病毒等混入检验标本，影响检验效果，使检验结果不精确，为此，检验科的杀菌消毒工作十分重要。
(三)发明内容
本发明为了弥补现有技术的不足，提供了一种制备简单、疗效显著的一种检验科用中药杀菌液。
本发明是通过如下技术方案实现的：
一种检验科用中药杀菌液，制成所述药物有效成分的原料组成及重量份数为：金银花10-25份，鱼腥草10-26份，黄连12-26份，板蓝根10-25份，柴胡10-26份，黄芩11-25份，花椒10-25份，连翘10-26份，皂角12-26份，菊花10-25份。
制成所述药物有效成分的原料组成及重量份数为：金银花10份，鱼腥草10份，黄连12份，板蓝根10份，柴胡10份，黄芩11份，花椒10份，连翘10份，皂角12份，菊花10份。
将上述中药清洗后煎煮，滤渣、放凉后，按照1∶9的比例与75％的酒精溶液混合，混合均匀后静置、滤渣加入辅助药物，利用现代先进的制药科技，制成杀菌洗液，即为所述的检验科用中药杀菌护理液。
本发明所用中药的药性如下：
金银花：有广谱抗菌作用，对金黄色葡萄球菌、白色葡萄球菌、伤寒杆菌、志贺氏痢疾杆菌、肺炎双球菌等都有抑制作用。
鱼腥草：对溶血性链球菌、金黄色葡萄球菌、流感杆菌、大肠杆菌、痢疾杆菌、伤寒杆菌有较强的抑制作用。鱼腥草有很好的抗病毒效果，急性细菌性角膜炎和流行性病毒性结膜炎都可以用鱼腥草滴眼液治疗，还能抑制霉菌，对宫颈炎有一定的治疗作用。此外，肺炎、泌尿系感染、肾炎、中耳炎治疗也可以用鱼腥草。
黄连：根茎入药，对脑膜炎双球菌、痢疾杆菌、炭疽杆菌、乙肝病毒、阴道滴虫等都有抑制作用。
板蓝根：对感冒病毒有抑制作用，对枯草杆菌、大肠杆菌、伤寒杆菌、痢疾杆菌、肠炎杆菌等也有杀菌作用。
柴胡：对伤寒杆菌、大肠杆菌、酵母菌等所致的发热有明显解热作用，同时能抑制结核杆菌、流感杆菌和牛痘病毒，还能杀死疟原虫。
黄芩：对皮肤真菌、白喉杆菌、结核杆菌、霍乱弧菌、痢疾杆菌和白色念珠菌都有抑制作用，即使对青霉素已经产生耐药性的金黄色葡萄球菌，黄芩仍然有效。
花椒：花椒果实含挥发油，挥发油中含柠檬烯(Limonene)、枯醇(Cumicalcohol).亦含有牻牛儿醇(Geraniol)、植物甾醇(Phytosterol)及不饱和脂肪酸.青川椒果实含挥发油，油中含异茴香脑(草蒿脑Estragolen，Methyl-chavicol)、佛手柑内酯(Bergapten)和苯甲酸(Benzoicacid)挥发油中尚含α-蒎烯、β-蒎烯、香桧萜、β-水芹烯、β-罗勒烯-X、α-萜品醇、樟醇、萜品烯醇-4、α-萜品醇、反式-石竹烯、乙酸萜品醇、葎草烯、β-荜澄茄油烯、橙花椒醇异构体等；芳香健胃、温中散寒、除湿止痛、杀虫解毒、止痒解腥等功效，主要治疗呕吐、风寒湿痹、齿痛等症。
连翘：归肺、心、胆经。连翘有广谱抗菌作用，对金黄色葡萄球菌、贺氏痢疾杆菌有很强的抑制作用，对其他致病菌、流感病毒、真菌都有一定抑制作用。有抗炎作用。
皂角：皂角又称皂荚，荚果、种子、枝刺等均可入药，荚果入药可祛痰、利尿；种子入药可治癣和通便秘；皂刺入药可活血并治疮癣，皂荚树以果实、种子入药。皂角能刺激胃黏膜而反射性的促进呼吸道黏液分泌，产生祛痰作用。皂角对很多细菌、真菌有天然的抑制和杀菌的作用。
菊花：疏风散寒，抗菌消炎；【化学成分】花和茎含挥发油，并有腺嘌呤、胆碱、水苏碱等。花又含菊甙、氨基酸、黄酮类及微量维生素B1。挥发油主要含龙脑、樟脑、菊油环酮等。黄酮类有木犀草素-7-葡萄糖甙、大波斯菊甙、刺槐甙。尚含丁二酸二甲基酰肼。【药理作用】①抗病原体作用菊花在体外对革兰氏阳性细菌(金黄色葡萄球菌及β-溶血性链球菌)、人型结核杆菌有某些抑制作用。其水浸剂(1∶4)对某些常见皮肤致病性真菌亦有些抑制作用。高浓度在体外还有抗病毒(PB8株)及抗螺旋体作用。②增强毛细血管抵抗力菊花提取物刚、鼠腹腔注射，可使皮内注射组织胺之局部台盼蓝之扩散较小，显示其能抑制毛细血管的通透性而有抗炎作用。其提取物10毫克相当芦丁2.5毫克之效力。【性味】甘苦，凉。【功用主治-菊花的功效】疏风，清热，明目，解毒。治头痛，眩晕，目赤，心胸烦热，疔疮，肿毒。①《本经》：主诸风头眩、肿痛，目欲脱，泪出，皮肤死肌，恶风湿痹，利血气。②《别录》：疗腰痛去来陶陶，除胸中烦热，安肠胃，利五脉，调四肢。③陶弘景：白菊：主风眩。④《药性论》：能治热头风旋倒地，脑骨疼痛，身上诸风令消散。⑤《日华子本草》：利血脉，治四肢游风，心烦，胸膈壅闷，并痈毒，头痛；作枕明目。⑥《珍珠囊》：养目血。⑦《用药心法》：去翳膜，明目。⑧王好古：主肝气不足。⑨《纲目拾遗》：专入阳分。治诸风头眩，解酒毒疔肿。黄茶菊：明目祛风，搜肝气，治头晕目眩，益血润容，入血分；白茶菊：通肺气，止咳逆，清三焦郁火，疗肌热，入气分。
本发明配制简单，吸收效果好，药效持续时间长，疗效显著，无副作用，安全性高，生产成本低，具有杀菌能力强，作用速度快，稳定性好的显著优点，适应于检验科以及医院其他科室的杀菌消毒，不污染环境，对人和动物无刺激，无毒副作用，是一种值得推广的理想杀菌药物。
(四)具体实施方式
实施例1：
取金银花25份，鱼腥草26份，黄连26份，板蓝根25份，柴胡26份，黄芩25份，花椒25份，连翘26份，皂角26份，菊花25份，将上述中药清洗后煎煮，滤渣、放凉后，按照1∶9的比例与75％的酒精溶液混合，混合均匀后静置、滤渣加入辅助药物，利用现代先进的制药科技，制成杀菌洗液，即为所述的检验科用中药杀菌护理液。
实施例2：
取金银花10份，鱼腥草10份，黄连12份，板蓝根10份，柴胡10份，黄芩11份，花椒10份，连翘10份，皂角12份，菊花10份，将上述中药清洗后煎煮，滤渣、放凉后，按照1∶9的比例与75％的酒精溶液混合，混合均匀后静置、滤渣加入辅助药物，利用现代先进的制药科技，制成杀菌洗液，即为所述的检验科用中药杀菌护理液。
实施例3：
取金银花18份，鱼腥草19份，黄连19份，板蓝根17份，柴胡15份，黄芩15份，花椒16份，连翘16份，皂角18份，菊花16份，将上述中药清洗后煎煮，滤渣、放凉后，按照1∶9的比例与75％的酒精溶液混合，混合均匀后静置、滤渣加入辅助药物，利用现代先进的制药科技，制成杀菌洗液，即为所述的检验科用中药杀菌护理液。
临床实验：
1、实验菌种：金黄色葡萄球菌、绿脓杆菌、大肠杆菌。
2、菌种培养基：营养琼脂培养基和营养肉汤培养基。
3、抑菌实验分实验组和对照组，实验组为本发明杀菌药物，对照组选用氯酸钠消毒液。
4、具体实验步骤：
(1)制备菌液：将上述两组菌种分别接种于两个琼脂培养基上，在37℃下培养24小时，分别从中挑取典型菌落分别接种于两个新鲜的营养肉汤培养基中，同温度下，培养18小时，备用；
(2)分别取本发明杀菌药物和氯酸钠消毒液各100ml，分别置于稀释容器中，对其分别稀释20倍，将上两种稀释液溶液分别分置两个容器内，置于无菌条件下，备用；
(3)将上述步骤(1)经营养肉汤培养18小时的实验菌种用无菌的营养肉汤分别稀释100倍，每种菌种分装于两个培养皿中，分别标记对照组和实验组；
(4)将上述步骤(2)所得的本发明消毒液的稀释溶液滴入实验组培养皿，氯酸钠消毒液稀释溶液滴入对照组培养皿。
5、实验观察测量：
| 菌种 | 实验组 | 对照组 | 菌落残留面积 |
| 金黄色葡萄球菌 | 2.5％ | 25.9％ | - |
| 绿脓杆菌 | 3.2％ | 32％ | - |
| 大肠杆菌 | 1.3％ | 27.3％ | - |
对上述实验进行观察并测量菌种残留面积，具体结果如上表。
6、观测测量完毕后，将上述培养皿均置于无菌条件下静置三天，跟踪观察发现，对照组菌落继续繁殖，而实验组菌落无变化。
7、实验结果：
实验组的杀菌能力远大于对照组的杀菌能力，且能够抑制菌种继续繁殖。
Claims (3)
1.一种检验科用中药杀菌液，其特征为，制成所述药物有效成分的原料组成及重量份数为：金银花10-25份，鱼腥草10-26份，黄连12-26份，板蓝根10-25份，柴胡10-26份，黄芩11-25份，花椒10-25份，连翘10-26份，皂角12-26份，菊花10-25份。
2.根据权利要求1所述的一种检验科用中药杀菌液，其特征为，制成所述药物有效成分的原料组成及重量份数为：金银花10份，鱼腥草10份，黄连12份，板蓝根10份，柴胡10份，黄芩11份，花椒10份，连翘10份，皂角12份，菊花10份。
3.根据权利要求1或2所述的治一种检验科用中药杀菌液，其特征在于：所述将上述中药清洗后煎煮，滤渣、放凉后，按照1∶9的比例与75％的酒精溶液混合，混合均匀后静置、滤渣加入辅助药物，利用现代先进的制药科技，制成杀菌洗液，即为所述的检验科用中药杀菌护理液。
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