CN104388450A - Heterologous expression of GH11 heat-resistant xylanase gene - Google Patents
Heterologous expression of GH11 heat-resistant xylanase gene Download PDFInfo
- Publication number
- CN104388450A CN104388450A CN201410758794.8A CN201410758794A CN104388450A CN 104388450 A CN104388450 A CN 104388450A CN 201410758794 A CN201410758794 A CN 201410758794A CN 104388450 A CN104388450 A CN 104388450A
- Authority
- CN
- China
- Prior art keywords
- syxyl11
- gene
- enzyme
- activity
- pet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides an expression, activity assay and enzymatic property analysis method of a codon optimization gene originated from Thermobifida fusca GH11 heat-resistant xylanase in escherichia coli. The gene is called after Syxyl11, the nucleotide sequence of the gene is SEQ ID NO: 1, the coded protein is SyXyl11, and the amino acid sequence is SEQ ID NO: 2. The enzymatic activity is 47.5 U/ml; the optimal reaction temperature is 70 DEG C, the temperature is preserved for 60 min at 70 DEG C, and the residual enzyme activity is 78.3%, so that the heat stability of the enzyme is better; the optimum pH (Potential of Hydrogen) is 6.0, and the pH is relatively stable in the range of 5.0-8.0; the influence of metal ions on the enzymatic activity is relatively little. The method establishes a theoretical basis for industrialized production of the enzyme, and has higher industrialized application potential and economic values.
Description
Technical field
The present invention relates to one derive from thermophilic split glycoside hydrolase 11 family heat resistant xylanase (SyXyl11) gene of spore bacterium (Thermobifida fusca) heterogenous expression, determination of activity and characterization analysis method, belong to technical field of bioengineering.
Background technology
Mierocrystalline cellulose, hemicellulose and xylogen are the main components of plant cell wall, and xylan is the important component part of plant hemicellulose, and it is the renewable biological source that content second is abundant after Mierocrystalline cellulose.Zytase is the general name of one group of enzyme of degradation of xylan, because the source of xylan is different, the complicacy of its structure is also different, the participation acting in conjunction of its multiple lytic enzyme of degradable needs completes, as endo-xylanase, β-D-xylosidase, glucuronic acid enzyme and α-L-arabinofuranosidase etc.Wherein the most important thing is β-1,4-endo-xylanase (endo-β-1,4-xylanase, EC 3.2.1.8), it can from the internal random cutting wood sugar glycosidic bond of xylan backbone, be degraded into oligomeric xylose, xylo-bioses and a small amount of wood sugar, be hemicellulose class resource conversion and utilize indispensable biological catalyst.Zytase can be divided into glycoside hydrolase 10 family and 11 families according to the amino acid whose homology of catalyst structure domain and hydrophobic cluster analysis method.Zytase has important using value at industrial circles such as papermaking, food, feed, weavings, but because the technique such as association with pulp bleaching, feed granulating all needs comparatively high temps, therefore the research and development of heat resistant xylanase receives the concern of Many researchers.Found the microorganism of many Neng Chan 11 families heat resistant xylanase at present, wherein many heat resistant xylanase genes have been cloned and have expressed in suitable host.According to the Preference of codon, host cell can with different velocity composite protein.The tRNA content that in the gene of coded protein, some codon is corresponding is few (namely belonging to rare codon), so resultant quantity is less, host controls the resultant velocity of protein with this.Therefore according to the Preference of codon, the codon optimized expression amount that effectively can improve foreign protein is carried out to foreign protein encoding gene.Current escherichia expression system is with low cost with it, productivity is high, simple operation and other advantages enjoys favor.
Summary of the invention
The object of this invention is to provide a kind of derive from thermophilic split GH11 heat resistant xylanase (SyXyl11) gene of spore bacterium heterogenous expression, determination of activity and characterization analysis method, be allos high expression and the industrialization production based theoretical of this enzyme.
Technical scheme of the present invention: carry out sequence optimisation according to e. coli codon Preference by being derived from the thermophilic gene xyl11 catalytic domain splitting the GH11 heat resistant xylanase of spore bacterium, after optimizing, unnamed gene is Syxyl11, its nucleotides sequence is classified as SEQ IDNO:1, the protein of coding is SyXyl11, and its aminoacid sequence is SEQ ID NO:2; This optimized gene is expressed in intestinal bacteria (Escherichia coli) BL21 (DE3), and determination of activity and characterization analysis are carried out to expression product.
Described xyl11 is that one derives from the thermophilic heat resistant xylanase gene (GenBank accession number is AY795559) splitting spore bacterium (Thermobifida fusca NTU22), its thermostability is very good, has the ideal behavior being suitable for industrial application.
The heterogenous expression of described SyXyl11, determination of activity and characterization analysis method:
(1) structure of recombinant plasmid pUCm-T-Syxyl11: according to the Preference of e. coli codon, carry out codon optimized to deriving from thermophilic heat resistant xylanase gene xyl11 catalytic domain (570bp) splitting spore bacterium, and adding EcoR I, Not I site respectively at its catalytic domain gene two ends, this unnamed gene is Syxyl11; This gene rear clone of synthetic is to pUCm-T plasmid, and Transformed E .coli JM109, cuts after qualification correctly through enzyme and serve Hai Shenggong order-checking, obtain recombinant plasmid pUCm-T-Syxyl11.
(2) structure of recombinant expression plasmid: recombinant plasmid pUCm-T-Syxyl11 obtained above is carried out EcoR I and Not I double digestion, rubber tapping is reclaimed product and is connected with the expression plasmid pET-28a (+) through same double digestion, through heat-shock transformed method, pET-28a-Syxyl11 is imported in E.coli BL21 (DE3), the LB coated containing kantlex is dull and stereotyped, filter out positive transformant through PCR, serve Hai Shenggong order-checking.The recombinant expression plasmid called after pET-28a-Syxyl11 checking order correct, positive transformant called after BL21-pET-28a-Syxyl11.
(3) expression of Syxyl11 in intestinal bacteria and product purification: positive transformant BL21-pET-28a-Syxyl11 is inoculated in 2mL containing in the LB substratum of Kan, 37 DEG C of shaking culture are spent the night, inoculum size with 1% is transferred in 30mL same medium, and 37 DEG C of shaking culture are to mid log phase (OD
600=0.6 ~ 0.8), adding IPTG to final concentration is 0.5mmol/L, at 20 DEG C of inducing culture 8h.By bacterial sediment, with citric acid-Na
2hPO
4damping fluid (pH 6.0) suspends, ice bath sonicated cells, centrifugal that supernatant be crude enzyme liquid.With Metal immobilization affinity chromatography column purification target protein.
(4) determination of activity of zytase and analysis: xylanase activity measures the DNS reagent method adopting and improve.Add the enzyme liquid that 0.1mL suitably dilutes in 2.4mL 0.5% birch xylan solution, react 15min under optimum temperuture, add 2.5mL DNS reagent, boiling water bath colour developing 7min, measures OD
540.Under the above-described reaction conditions, unit of enzyme activity (U) is defined as per minute and produces enzyme amount needed for 1 μm of ol reducing sugar.Protein content determination adopts dying method with coomassie brilliant blue (Bradford method), and adopts SDS-PAGE to analyze expression product and the mensuration of apparent molecular weight.
(5) recombined xylanase zymologic property measures and analyzes:
The optimum temperuture of enzyme and thermostability: get suitably dilution enzyme liquid and carry out enzyme digestion reaction under 50 ~ 80 DEG C of conditions, every 5 DEG C, measure enzyme respectively and live, with enzyme activity soprano for 100%, make temperature-relative activity curve; After enzyme liquid is incubated 1h under differing temps, measure residual enzymic activities according to a conventional method, to be incubated the enzymic activity of enzyme liquid for 100%, make temperature-relative activity curve.When remnant enzyme activity reaches more than 85%, be namely defined as stable.
The optimal pH of enzyme and pH stability: under optimum temperuture, measure the enzymic activity of zytase under different pH value.With enzyme activity soprano for 100%, make pH-relative activity curve; Enzyme is incubated 1h in 40 DEG C under different pH value condition, then measures residual enzymic activities respectively, with the enzymic activity of untreated enzyme liquid for 100%, make pH-relative activity curve.When remnant enzyme activity reaches more than 85%, be namely defined as stable.
Metal ion is on the impact of enzymic activity: mixed with different metal solion by enzyme liquid, and final concentration is 5mmol/L, 40 DEG C, and insulation 1h, then measures residual enzymic activities according to a conventional method, be defined as 100% with the enzymic activity not adding metal ion.
Beneficial effect of the present invention: the invention provides a kind of be derived from thermophilic split GH11 heat resistant xylanase (SyXyl11) gene of spore bacterium heterogenous expression, determination of activity and characterization analysis method, its corresponding gene is Syxyl11, the optimal reactive temperature of recombinase is 70 DEG C, 60min is incubated at 70 DEG C, remnant enzyme activity is 78.3%, shows that the thermostability of this enzyme is relatively good; The present invention is that theoretical basis has been established in the industrialization production of this enzyme, has larger industrial applications potentiality and economic worth, also for theoretical basis has been established in the research of other heat resistant xylanase.
Embodiment
Below in conjunction with specific embodiment, set forth working method of the present invention further.But these embodiments are only for describing the present invention in detail, and are not used in and limit the scope of the invention.
The structure of embodiment 1 recombinant plasmid pUCm-T-Syxyl11
For realizing deriving from the thermophilic high expression of heat resistant xylanase gene xyl11 in E.coli splitting spore bacterium, e. coli codon optimization is carried out to xyl11 catalytic domain gene order (570bp), and add EcoR I, Not I site respectively at its catalytic domain gene two ends, this unnamed gene is Syxyl11, synthetic rear clone is to pUCm-T plasmid, Transformed E .coli JM109, cuts after qualification correctly through enzyme and serves Hai Shenggong order-checking, obtain recombinant plasmid pUCm-T-Syxyl11.
The structure of embodiment 2 recombinant expression plasmid
Recombinant plasmid pUCm-T-Syxyl11 obtained above is carried out EcoR I and Not I double digestion, the goal gene Syxyl11 of about 570bp is reclaimed in rubber tapping, connect with the expression plasmid pET-28a (+) through same double digestion, through heat-shock transformed method, pET-28a-Syxyl11 is imported in E.coli BL21 (DE3) competent cell.The LB coated containing kantlex is dull and stereotyped, through universal primer (T
7-F and T
7-R) bacterium liquid PCR serve after filtering out positive transformant Hai Shenggong order-checking, the recombinant expression plasmid called after pET-28a-Syxyl11 checking order correct, positive transformant called after BL21-pET-28a-Syxyl11.
The expression of embodiment 3Syxyl11 in E.coli BL21 (DE3) and product purification
BL21-pET-28a-Syxyl11 is inoculated in 2mL containing in the LB substratum of 100 μ g/mL kantlex (Kan), in 37 DEG C, 220r/min shaking culture 12h, then transfer in 30mL containing in the fresh LB of Kan with the inoculum size of 1%, 220r/min, treats OD
600when reaching 0.8, adding IPTG to final concentration is 0.5mmol/L, 20 DEG C of inducing culture 8h.By bacterial sediment, with 15mL citric acid-Na
2hPO
4damping fluid (pH 6.0) suspends, ice bath sonicated cells, and centrifugal that supernatant be crude enzyme liquid, enzymic activity is 47.5U/mL.At 4 DEG C, with 2mL Metal immobilization affinity chromatography column purification target protein, adopting Bradford method to measure protein content, is 55U/mg than enzymic activity; Adopt SDS-PAGE to analyze expression product, the apparent molecular weight of zymoprotein is 24.8kDa.
Embodiment 4 recombined xylanase characterization analysis
The optimum temperuture of enzyme and thermostability: get suitably dilution enzyme liquid and carry out enzyme digestion reaction under 50 ~ 80 DEG C of conditions, every 5 DEG C, measure enzyme respectively and live, with enzyme activity soprano for 100%, make temperature-relative activity curve, its optimum temperuture is 70 DEG C; After enzyme liquid is incubated 1h under differing temps, measure residual enzymic activities according to a conventional method, not to be incubated the enzymic activity of enzyme liquid for 100%, make temperature-relative activity curve, this recombinase is incubated 60min at 70 DEG C, and remnant enzyme activity is 78.3%.
The optimal pH of enzyme and pH stability: at optimum temperuture 70 DEG C, measure the enzymic activity of zytase under different pH value (0.2mol/L citrate-phosphate disodium hydrogen damping fluid: pH 3.0 ~ 8.0 and This-HCl damping fluid: pH 8.5-9.0) respectively.With enzyme activity soprano for 100%, make pH-relative activity curve, its optimal pH is 6.0; Enzyme is incubated 1h in 40 DEG C under different pH value condition, then measures residual enzymic activities respectively, with the enzymic activity of untreated enzyme liquid for 100%, make pH-relative activity curve, show this enzyme at pH 5.0 ~ 8.0 range stabilises.
Metal ion is on the impact of enzymic activity: mixed with different metal solion (final concentration is 5mmol/L) by enzyme liquid, 40 DEG C, insulation 1h, then residual enzymic activities is measured according to a conventional method, be defined as 100% with the enzymic activity not adding metal ion, the impact of result display metal ion on this enzymic activity is less.
Claims (2)
1. one kind derives from the thermophilic heat resistant xylanase catalytic domain optimized gene (Syxyl11) splitting spore bacterium (Thermobifida fusca) NTU22 bacterial strain glycoside hydrolase the 11st family (GH11), and the nucleotide sequence of its correspondence and aminoacid sequence are respectively SEQ IDNO:1 and SEQ ID NO:2.
The mensuration of the heterogenous expression of 2.SyXyl11, enzymic activity and enzymatic property:
(1) structure of recombinant plasmid pUCm-T-Syxyl11: according to the Preference of e. coli codon, carry out codon optimized to deriving from thermophilic heat resistant xylanase gene xyl11 catalytic domain (570bp) splitting spore bacterium, and adding EcoR I, Not I site respectively at its catalytic domain gene two ends, this unnamed gene is Syxyl11; This gene rear clone of synthetic is to pUCm-T plasmid, and Transformed E .coli JM109, serves Hai Shenggong order-checking, obtain recombinant plasmid pUCm-T-Syxyl11 after PCR checking;
(2) structure of recombinant expression plasmid: recombinant plasmid pUCm-T-Syxyl11 obtained above is carried out EcoR I and Not I double digestion, rubber tapping is reclaimed product and is connected with the expression plasmid pET-28a (+) through same double digestion, through heat-shock transformed method, pET-28a-Syxyl11 is imported in E.coli BL21 (DE3), the LB coated containing kantlex (Kan) is dull and stereotyped, filter out positive transformant through PCR, serve Hai Shenggong order-checking; The recombinant expression plasmid called after pET-28a-Syxyl11 checking order correct, positive transformant called after BL21-pET-28a-Syxyl11;
(3) mensuration of the expression of Syxyl11 in intestinal bacteria, product purification and enzymatic property:
Positive transformant BL21-pET-28a-Syxyl11 is inoculated in 2mL containing in the LB substratum of Kan, 37 DEG C of shaking culture are spent the night, and the inoculum size with 1% is transferred in 30mL same medium, and 37 DEG C of shaking culture are to mid log phase (OD
600be 0.6 ~ 0.8), adding IPTG to final concentration is 0.5mmol/L, 20 DEG C of inducing culture 8h.By bacterial sediment, with citric acid-Na
2hPO
4damping fluid (pH 6.0) suspends, ice bath sonicated cells, centrifugal that supernatant be crude enzyme liquid; It is 47.5U/mL that DNS method records recombined xylanase activity in crude enzyme liquid; With Metal immobilization affinity chromatography column purification target protein, after purifying, be detected as single band through SDS-PAGE, and to show recombined xylanase molecular weight be 24.8kDa; Adopting Bradford method to measure protein content, is 55U/mg than enzymic activity; The optimal reactive temperature of this enzyme is 70 DEG C, is incubated 60min at 70 DEG C, and remnant enzyme activity is 78.3%; Its optimal pH is 6.0, more stable in pH 5.0 ~ 8.0 scope; Metal ion is less on the impact of this enzymic activity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410758794.8A CN104388450A (en) | 2014-12-10 | 2014-12-10 | Heterologous expression of GH11 heat-resistant xylanase gene |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410758794.8A CN104388450A (en) | 2014-12-10 | 2014-12-10 | Heterologous expression of GH11 heat-resistant xylanase gene |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104388450A true CN104388450A (en) | 2015-03-04 |
Family
ID=52606407
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410758794.8A Pending CN104388450A (en) | 2014-12-10 | 2014-12-10 | Heterologous expression of GH11 heat-resistant xylanase gene |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104388450A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104946614A (en) * | 2015-06-18 | 2015-09-30 | 江南大学 | Preparation of recombinant xylanase and method for applying recombinant xylanase to cassava residue degradation |
| CN106636038A (en) * | 2016-10-31 | 2017-05-10 | 江南大学 | Heat resistance improved xylanases and application thereof |
| CN108251403A (en) * | 2016-12-29 | 2018-07-06 | 上海交通大学 | A kind of novel glycosyl sphingolipid endoglycosidase and its gene engineering preparation method and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102392033A (en) * | 2011-12-12 | 2012-03-28 | 江南大学 | Beta-1, 4-endo-xylanase (Aor Xyn 11A) gene cloning and preparation of recombinase |
| CN102703481A (en) * | 2012-06-05 | 2012-10-03 | 江南大学 | Method for heat resistance modification of aspergillus oryzae GH11 xylanase gene and preparation of mutant enzyme |
| WO2014005499A1 (en) * | 2012-07-02 | 2014-01-09 | Novozymes A/S | Polypeptides having xylanase activity and polynucleotides encoding same |
| CN103642776A (en) * | 2013-12-10 | 2014-03-19 | 江南大学 | Directed evolution method for improving heat resistance of aspergillus oryzae GH11 xylanase |
-
2014
- 2014-12-10 CN CN201410758794.8A patent/CN104388450A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102392033A (en) * | 2011-12-12 | 2012-03-28 | 江南大学 | Beta-1, 4-endo-xylanase (Aor Xyn 11A) gene cloning and preparation of recombinase |
| CN102703481A (en) * | 2012-06-05 | 2012-10-03 | 江南大学 | Method for heat resistance modification of aspergillus oryzae GH11 xylanase gene and preparation of mutant enzyme |
| WO2014005499A1 (en) * | 2012-07-02 | 2014-01-09 | Novozymes A/S | Polypeptides having xylanase activity and polynucleotides encoding same |
| CN103642776A (en) * | 2013-12-10 | 2014-03-19 | 江南大学 | Directed evolution method for improving heat resistance of aspergillus oryzae GH11 xylanase |
Non-Patent Citations (2)
| Title |
|---|
| CHENG, Y.F ET AL: "ACCESSION NO AY795559.1", 《GENBANK》 * |
| 殷尔康: "疏棉状嗜热丝孢菌木聚糖酶A在大肠杆菌中的高效表达", 《中国优秀硕士学位论文全文数据库.基础科学辑》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104946614A (en) * | 2015-06-18 | 2015-09-30 | 江南大学 | Preparation of recombinant xylanase and method for applying recombinant xylanase to cassava residue degradation |
| CN104946614B (en) * | 2015-06-18 | 2018-11-02 | 江南大学 | A kind of method of recombined xylanase prepared and its degrade applied to manioc waste |
| CN106636038A (en) * | 2016-10-31 | 2017-05-10 | 江南大学 | Heat resistance improved xylanases and application thereof |
| CN106636038B (en) * | 2016-10-31 | 2019-12-20 | 江南大学 | Xylanase with improved heat resistance and application thereof |
| CN108251403A (en) * | 2016-12-29 | 2018-07-06 | 上海交通大学 | A kind of novel glycosyl sphingolipid endoglycosidase and its gene engineering preparation method and application |
| CN108251403B (en) * | 2016-12-29 | 2021-05-28 | 上海交通大学 | Novel glycosphingolipid endoglycosidase and genetic engineering preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Bala et al. | Cellulolytic and xylanolytic enzymes of thermophiles for the production of renewable biofuels | |
| Pei et al. | Thermoanaerobacterium thermosaccharolyticum β-glucosidase: a glucose-tolerant enzyme with high specific activity for cellobiose | |
| Liu et al. | Characterization of a thermostable β-glucosidase from Aspergillus fumigatus Z5, and its functional expression in Pichia pastoris X33 | |
| Hu et al. | Cloning and enzymatic characterization of a xylanase gene from a soil-derived metagenomic library with an efficient approach | |
| Bussamra et al. | Improvement on sugar cane bagasse hydrolysis using enzymatic mixture designed cocktail | |
| Mechelke et al. | Characterization of the arabinoxylan-degrading machinery of the thermophilic bacterium Herbinix hemicellulosilytica—six new xylanases, three arabinofuranosidases and one xylosidase | |
| Banka et al. | Secretory expression and characterization of two hemicellulases, xylanase, and β-xylosidase, isolated from Bacillus subtilis M015 | |
| Zhang et al. | Cloning, expression, and characterization of an alkaline thermostable GH11 xylanase from Thermobifida halotolerans YIM 90462T | |
| Su et al. | Reconstitution of a thermostable xylan-degrading enzyme mixture from the bacterium Caldicellulosiruptor bescii | |
| KR20150079676A (en) | Beta-glucosidase from magnaporthe grisea | |
| Liao et al. | A new acidophilic endo-β-1, 4-xylanase from Penicillium oxalicum: cloning, purification, and insights into the influence of metal ions on xylanase activity | |
| Amaya-Delgado et al. | Cloning and expression of a novel, moderately thermostable xylanase-encoding gene (Cfl xyn11A) from Cellulomonas flavigena | |
| Teeravivattanakit et al. | Novel trifunctional xylanolytic enzyme Axy43A from Paenibacillus curdlanolyticus strain B-6 exhibiting endo-xylanase, β-D-xylosidase, and arabinoxylan arabinofuranohydrolase activities | |
| Sun et al. | Direct cloning, expression of a thermostable xylanase gene from the metagenomic DNA of cow dung compost and enzymatic production of xylooligosaccharides from corncob | |
| WO2016069541A1 (en) | Compositions and methods related to beta-glucosidase | |
| Wang et al. | A new xylanase from thermoalkaline Anoxybacillus sp. E2 with high activity and stability over a broad pH range | |
| de Sousa Gomes et al. | Purification and characterization of xylanases from the fungus Chrysoporthe cubensis for production of xylooligosaccharides and fermentable sugars | |
| Yin et al. | Expression and characteristics of two glucose-tolerant GH1 β-glucosidases from Actinomadura amylolytica YIM 77502T for promoting cellulose degradation | |
| Li et al. | Functional expression and synergistic cooperation of xylan‐degrading enzymes from Hypocrea orientalis and Aspergillus niger | |
| MX2015005424A (en) | Beta-glucosidase from neurospora crassa. | |
| Sheng et al. | Discovery and characterization of endo-xylanase and β-xylosidase from a highly xylanolytic bacterium in the hindgut of Holotrichia parallela larvae | |
| Tu et al. | A GH51 α-L-arabinofuranosidase from Talaromyces leycettanus strain JCM12802 that selectively drives synergistic lignocellulose hydrolysis | |
| Nakazawa et al. | A high performance Trichoderma reesei strain that reveals the importance of xylanase III in cellulosic biomass conversion | |
| Zhao et al. | Two family 11 xylanases from Achaetomium sp. Xz-8 with high catalytic efficiency and application potentials in the brewing industry | |
| Wongratpanya et al. | Multifunctional properties of glycoside hydrolase family 43 from Paenibacillus curdlanolyticus strain B-6 including exo-β-xylosidase, endo-xylanase, and α-L-arabinofuranosidase activities |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150304 |
|
| WD01 | Invention patent application deemed withdrawn after publication |