CN104311170A - Method for producing organic fertilizer through biodegradation of died livestock - Google Patents

Method for producing organic fertilizer through biodegradation of died livestock Download PDF

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Publication number
CN104311170A
CN104311170A CN201410516632.3A CN201410516632A CN104311170A CN 104311170 A CN104311170 A CN 104311170A CN 201410516632 A CN201410516632 A CN 201410516632A CN 104311170 A CN104311170 A CN 104311170A
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China
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subtilis
content
volume
activation
bacillus
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CN201410516632.3A
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Chinese (zh)
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韩剑众
曲道峰
吴劲松
余全法
竺礼斌
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浙江工商大学
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Priority to CN201410516632.3A priority Critical patent/CN104311170A/en
Publication of CN104311170A publication Critical patent/CN104311170A/en

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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F1/00Fertilisers made from animal corpses, or parts thereof
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/70Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry

Abstract

The invention discloses a method for producing an organic fertilizer through biodegradation of died livestock. The method comprises the steps of fully mixing died livestock bodies with a compound microbial agent according to a proportion of 100Kg: (30-45L), then fully mixing with chicken manure, sludge and straw, putting into a fermentation tank for fermenting, and turning over in fermentation, wherein the ventilator capacity is 0.8-1.0VVM, the pressure of the tank is kept at 0.03-0.05Mpa, the temperature is at 55-62DEG C, and the period is 2-3 days. The organic fertilizer can reduce the cost of the fertilizer by over 65%, and can prolong the fertilizer nutrient release period to about 120%; due to adoption of the organic fertilizer, great pressure of the livestock died of disease on the environment can be lowered, so that the habitat balance is maintained; the compound microbial agent can ferment and degrade the livestock died of disease into organic fertilizer safely and efficiently in short time, so that the great pressure of the livestock died of disease on the environment can be lowered, furthermore, the waste is turned into wealth.

Description

A kind of method utilizing biological degradation dead livestock and poultry to prepare organic fertilizer
Technical field
The invention belongs to microbial technology field, relate to a kind of biological degradation dead livestock and poultry and prepare the method for organic fertilizer, the preparation method of complex micro organism fungicide and application.
Background technology
Popular and the outburst of the many animals epidemic disease that the modern farming enterprise's breeding production pattern of mass-producing causes, makes to eliminate and dead livestock and poultry quantity sharply increases; More because society is to eliminating and the problem such as the understanding of dead livestock and poultry harmless treatment, technology and cost, make to throw out without care everywhere everywhere and disorderly bury dead livestock and poultry event and constantly occur, this causes opportunity not only to the propagation of animal epidemic, and causes greatly pollution to ecotope and destroy.If utilize biological degradation dead livestock and poultry, dead livestock and poultry can not only be disposed safely, effectively, reduce environmental pollution, and its product can be used as land reclamation fertilizer.
Harmless biological process, as a kind of new system processing dead livestock and poultry, relative to the problem existing for current carcase innoxious process for treating, has safety, cost is low, pollution-free, the cycle is short, product can do the advantages such as land reclamation fertilizer.It under environmental friendliness, green condition, utilizes microorganism growth to ferment secrete efficient protein enzyme, dead livestock and poultry entirety degraded in biological treatment tank, finally become recycled small protein, amino acid whose process.The muscle, nerve, reticular tissue, blood etc. of animal are all basal component with protein.The skin, hair, plumage, pawl, angle etc. of animal are formed by keratoprotein.Protein is also the moiety of animal body endoenzyme, hormone, antibody, pigment, muscle and breast etc.Therefore, the key of biological treatment be find and cultivate can high proteinase yield and also can efficient-decomposition animal proteinum as the microorganism strains of Keratin sulfate, collagen protein.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, a kind of degradable dead livestock and poultry is provided, reduce environmental pollution, pathophoresis, utilize dead livestock and poultry to produce the method for bio-feritlizer.
The technical solution adopted for the present invention to solve the technical problems is as follows:
The inventive method comprises the following steps:
Step (1). dead livestock and poultry corpse is pulverized by pulverizer, then fully mixes with the weightmeasurement ratio of complex micro organism fungicide by 100 (Kg): 30 ~ 45 (L), obtain compound;
Described complex micro organism fungicide is by the subtilis-1(ZJNB0001 after cultivating), cultivate after subtilis-2 (ZJHZ0005), cultivate after Bacillus licheniformis (ZJHZ1025), bacillus cereus (ZJWZ0002) and the bacstearothermophilus (ZJNB0008) after cultivating form, the careless genus bacillus-1 after wherein cultivating, cultivate after subtilis-2, cultivate after Bacillus licheniformis, cultivate after bacillus cereus and the number ratio of bacstearothermophilus after cultivation be 3:2:2:2:1;
Described subtilis-1(ZJNB0001), the cultural method of subtilis-2 (ZJHZ0005) is as follows: being seeded to by the Bacillus subtilis strain cultivated at purifying (this subtilis refers to subtilis-1 or subtilis-2) and volume content is housed is in the shaking flask of 15 ﹪ activation mediums-1, the volume inoculum size of bacterial classification is 0.5 ~ 1 ﹪, culture temperature be 37 DEG C, shaking flask rotating speed cultivates 20h under being 180rpm; The Bacillus subtilis strain that activation culture is good is inoculated into and is equipped with in bio-reactor that volume content is 50 ﹪ substratum-1, the volume inoculum size of bacterial classification is 1 ﹪, culture temperature be 37 DEG C, air flow 8L/s, bio-reactor mixing speed cultivate 20h under being 220rpm, bacillus subtilis bacterial content>=10 in tank 9individual/ml;
The formula of described activation medium-1 is glucose 18g/l, peptone 12g/l, sodium-chlor 3g/l and extractum carnis 0.2g/l;
Described substratum-1 is filled a prescription as Semen Maydis powder 10g/l, glucose 3g/l, soybean cake powder 18g/l, fish meal 3g/l, calcium carbonate 5g/l, ammonium sulfate 0.8g/l, dipotassium hydrogen phosphate 0.1g/l, magnesium sulfate 0.1g/l and manganous sulfate 0.1g/l.
Described subtilis-1 comes from Zhejiang Prov Industrial And Commercial University's modern food safety and nutrition collaborative innovation center, and deposit number is ZJNB0001, and the public can buy and obtain; Described subtilis-2 comes from Zhejiang Prov Industrial And Commercial University's modern food safety and nutrition collaborative innovation center, and deposit number is ZJHZ0005, and the public can buy and obtain.
The cultural method of described Bacillus licheniformis (ZJHZ 1025) is as follows: being seeded to by the Bacillus licheniformis strain that purifying is cultivated and volume content is housed is in the shaking flask of 15 ﹪ activation mediums-2, the volume inoculum size of bacterial classification is 0.5 ~ 1 ﹪, culture temperature be 37 DEG C, shaking flask rotating speed cultivates 20h under being 180rpm; The Bacillus licheniformis strain that activation culture is good is inoculated into and is equipped with in bio-reactor that volume content is 50 ﹪ fermention mediums, the volume inoculum size of bacterial classification is 1 ﹪, culture temperature be 37 DEG C, air flow 8L/s, bio-reactor mixing speed cultivate 20h under being 200rpm, Bacillus licheniformis content>=10 in tank 9individual/ml;
The formula of described activation medium-2 is peptone 8g/l, extractum carnis 1g/l and sodium-chlor 3g/l;
Described fermentative medium formula is soybean cake powder 13g/l, Semen Maydis powder 13g/l, dipotassium hydrogen phosphate 0.05g/l, potassium primary phosphate 0.05g/l and calcium chloride 2g/l.
Described Bacillus licheniformis comes from Zhejiang Prov Industrial And Commercial University's modern food safety and nutrition collaborative innovation center, and deposit number is ZJHZ1025, and the public can buy and obtain.
Described bacillus cereus (ZJWZ0002) cultural method is as follows: the bacillus cereus strain inoculation of being cultivated by purifying is in the shaking flask of 15 ﹪ activation mediums-3 to being equipped with volume content, the volume inoculum size of bacterial classification is 0.5 ~ 1 ﹪, be 37 DEG C in culture temperature, shaking flask rotating speed is cultivate 20h under 180rpm; By bacillus cereus strain inoculation good for activation culture to being equipped with in bio-reactor that volume content is 50 ﹪ substratum-2, the volume inoculum size of bacterial classification is 1 ﹪, culture temperature be 37 DEG C, air flow 8L/s, bio-reactor mixing speed cultivate 20h under being 250rpm, bacillus cereus content>=10 in tank 9individual/ml.
Described activation medium-3 is filled a prescription as yeast extract paste 5 g/l, peptone 10 g/l, NaCl 5 g/l;
Described substratum-2 is filled a prescription as glucose 10 g/l, peptone 5 g/l, yeast extract paste 8 g/l, NaCl 5 g/l, K 2hP0 47 g/l, CaCl 22.5 g/l, MgS0 41 g/l, MnS0 40.01 g/l;
Described bacillus cereus comes from Zhejiang Prov Industrial And Commercial University's modern food safety and nutrition collaborative innovation center, and deposit number is ZJWZ0002, and the public can buy and obtain.
Described bacstearothermophilus (ZJNB0008) cultural method: the bacstearothermophilus strain inoculation of being cultivated by purifying is in the shaking flask of the activation medium-3 of 15 ﹪ to being equipped with volume content, the volume inoculum size of bacterial classification is 0.5 ~ 1 ﹪, culture temperature be 37 DEG C, shaking flask rotating speed cultivates 20h under being 180rpm; By bacstearothermophilus strain inoculation good for activation culture to being equipped with in bio-reactor that volume content is 50 ﹪ substratum-2, the volume inoculum size of bacterial classification is 1 ﹪, culture temperature be 50 DEG C, air flow 8L/s, bio-reactor mixing speed cultivate 20h under being 250rpm, bacstearothermophilus content>=10 in tank 9individual/ml;
Described activation medium-3 is filled a prescription as yeast extract paste extractum carnis 3 g/l, soy peptone 10 g/l, agar 16 g/l;
Described substratum-2 is filled a prescription as dregs of beans 5 g/l, yeast extract paste 8 g/l, K 2hP0 42 g/l.
Described bacstearothermophilus comes from Zhejiang Prov Industrial And Commercial University's modern food safety and nutrition collaborative innovation center, and deposit number is ZJNB0008, and the public can buy and obtain.
Step (2). put into fermentor tank after fully being mixed with chicken manure, mud, stalk by the compound that step (1) obtains and ferment at 55 ~ 62 DEG C, fermenting process carries out tipping operation, air flow 0.8 ~ 1.0 VVM, tank pressure keeps 0.03 ~ 0.05 Mpa, temperature remains on 55 ~ 62 DEG C, and the cycle is 2 ~ 3d; The fertilizer of rear mass content 80 ﹪ of having fermented can utilize making biological organic fertilizer, and the substrate of mass content 20 ﹪ can utilize again.
The total add-on of described chicken manure, mud, stalk is 30 ~ 40 ﹪ of the compound weight that step (1) obtains; In chicken manure, mud, stalk, the mass content of chicken manure is 10 ~ 25 ﹪, and the mass content of mud is 40 ~ 55 ﹪, and the mass content of stalk is 20 ~ 35 ﹪; Mud is the matrix as fertilizer; Chicken manure, stalk can be used as the substrate of biological fermentation, regulate carbon-nitrogen ratio, improve the content of N P and K simultaneously.
In the present invention, leavening temperature is 55 ~ 62 DEG C, is through experimental verification repeatedly, and complex micro organism fungicide fermentation efficiency in this temperature range is the highest, reacts the most complete; Fermentation period 2 ~ 3 days in the present invention, one is that two is in order to ensure eliminating pathogenic micro-organism completely in order to ensure that fermentation completely.
Beneficial effect of the present invention: organic fertilizer of the present invention can reduce use fertilizer cost 65 more than ﹪, extend fertilizer nutrient and reach about 120 ﹪ deenergized period, use the bio-organic fertilizer that this technology is produced, the immense pressure that dead livestock and poultry causes environment can be reduced, protection habitat balance; Complex micro organism fungicide of the present invention can short period of time safety, be organic fertilizer by dead livestock and poultry fermentative degradation efficiently, both the immense pressure that dead livestock and poultry causes environment had been decreased, can turn waste into wealth again, for the healthy and sustainable development ensureing livestock industry, food, ecological environment security tool are of great significance.
Embodiment
For further analysis to the present invention below in conjunction with specific embodiment.
The subtilis-1 used in embodiment below comes from Zhejiang Prov Industrial And Commercial University's modern food safety and nutrition collaborative innovation center, and deposit number is ZJNB0001, and the public can buy and obtain; Subtilis-2 comes from Zhejiang Prov Industrial And Commercial University's modern food safety and nutrition collaborative innovation center, and deposit number is ZJHZ0005, and the public can buy and obtain; Bacillus licheniformis comes from Zhejiang Prov Industrial And Commercial University's modern food safety and nutrition collaborative innovation center, and deposit number is ZJHZ1025, and the public can buy and obtain; Bacillus cereus comes from Zhejiang Prov Industrial And Commercial University's modern food safety and nutrition collaborative innovation center, and deposit number is ZJWZ0002, and the public can buy and obtain; Bacstearothermophilus comes from Zhejiang Prov Industrial And Commercial University's modern food safety and nutrition collaborative innovation center, and deposit number is ZJNB0008, and the public can buy and obtain.
Cultural method embodiment 1-1: subtilis-1(ZJNB0001)
Subtilis-1(ZJNB0001 by cultivating at purifying) strain inoculation is in the shaking flask of 15 ﹪ activation mediums-1 to being equipped with volume content, the volume inoculum size of bacterial classification is 0.5 ~ 1 ﹪, culture temperature be 37 DEG C, shaking flask rotating speed cultivates 20h under being 180rpm; The Bacillus subtilis strain that activation culture is good is inoculated into and is equipped with in bio-reactor that volume content is 50 ﹪ substratum-1, the volume inoculum size of bacterial classification is 1 ﹪, culture temperature be 37 DEG C, air flow 8L/s, bio-reactor mixing speed cultivate 20h under being 220rpm, bacillus subtilis bacterial content>=10 in tank 9individual/ml;
The formula of described activation medium-1 is glucose 18g/l, peptone 12g/l, sodium-chlor 3g/l and extractum carnis 0.2g/l;
Described substratum-1 is filled a prescription as Semen Maydis powder 10g/l, glucose 3g/l, soybean cake powder 18g/l, fish meal 3g/l, calcium carbonate 5g/l, ammonium sulfate 0.8g/l, dipotassium hydrogen phosphate 0.1g/l, magnesium sulfate 0.1g/l and manganous sulfate 0.1g/l.
Embodiment 1-2: the cultivation of subtilis-2 (ZJHZ0005)
Be in the shaking flask of 15 ﹪ activation mediums-1 by subtilis-2 (ZJHZ0005) strain inoculation of cultivating at purifying to being equipped with volume content, the volume inoculum size of bacterial classification is 0.5 ~ 1 ﹪, culture temperature be 37 DEG C, shaking flask rotating speed cultivates 20h under being 180rpm; The Bacillus subtilis strain that activation culture is good is inoculated into and is equipped with in bio-reactor that volume content is 50 ﹪ substratum-1, the volume inoculum size of bacterial classification is 1 ﹪, culture temperature be 37 DEG C, air flow 8L/s, bio-reactor mixing speed cultivate 20h under being 220rpm, bacillus subtilis bacterial content>=10 in tank 9individual/ml;
The formula of described activation medium-1 is glucose 18g/l, peptone 12g/l, sodium-chlor 3g/l and extractum carnis 0.2g/l;
Described substratum-1 is filled a prescription as Semen Maydis powder 10g/l, glucose 3g/l, soybean cake powder 18g/l, fish meal 3g/l, calcium carbonate 5g/l, ammonium sulfate 0.8g/l, dipotassium hydrogen phosphate 0.1g/l, magnesium sulfate 0.1g/l and manganous sulfate 0.1g/l.
Embodiment 1-3: the cultural method of Bacillus licheniformis (ZJHZ 1025)
Bacillus licheniformis (ZJHZ 1025) strain inoculation of being cultivated by purifying is in the shaking flask of 15 ﹪ activation mediums-2 to being equipped with volume content, the volume inoculum size of bacterial classification is 0.5 ~ 1 ﹪, culture temperature be 37 DEG C, shaking flask rotating speed cultivates 20h under being 180rpm; The Bacillus licheniformis strain that activation culture is good is inoculated into and is equipped with in bio-reactor that volume content is 50 ﹪ fermention mediums, the volume inoculum size of bacterial classification is 1 ﹪, culture temperature be 37 DEG C, air flow 8L/s, bio-reactor mixing speed cultivate 20h under being 200rpm, Bacillus licheniformis content>=10 in tank 9individual/ml;
The formula of described activation medium-2 is peptone 8g/l, extractum carnis 1g/l and sodium-chlor 3g/l;
Described fermentative medium formula is soybean cake powder 13g/l, Semen Maydis powder 13g/l, dipotassium hydrogen phosphate 0.05g/l, potassium primary phosphate 0.05g/l and calcium chloride 2g/l.
Embodiment 1-4: bacillus cereus (ZJWZ0002) cultural method
Bacillus cereus (ZJWZ0002) strain inoculation of being cultivated by purifying is in the shaking flask of 15 ﹪ activation mediums-3 to being equipped with volume content, and the volume inoculum size of bacterial classification is 0.5 ~ 1 ﹪, is 37 DEG C in culture temperature, and shaking flask rotating speed is cultivate 20h under 180rpm; By bacillus cereus strain inoculation good for activation culture to being equipped with in bio-reactor that volume content is 50 ﹪ substratum-2, the volume inoculum size of bacterial classification is 1 ﹪, culture temperature be 37 DEG C, air flow 8L/s, bio-reactor mixing speed cultivate 20h under being 250rpm, bacillus cereus content>=10 in tank 9individual/ml.
Described activation medium-3 is filled a prescription as yeast extract paste 5 g/l, peptone 10 g/l, NaCl 5 g/l;
Described substratum-2 is filled a prescription as glucose 10 g/l, peptone 5 g/l, yeast extract paste 8 g/l, NaCl 5 g/l, K 2hP0 47 g/l, CaCl 22.5 g/l, MgS0 41 g/l, MnS0 40.01 g/l;
Embodiment 1-5: bacstearothermophilus (ZJNB0008) cultural method:
Bacstearothermophilus (ZJNB0008) strain inoculation of being cultivated by purifying is in the shaking flask of the activation medium-3 of 15 ﹪ to being equipped with volume content, the volume inoculum size of bacterial classification is 0.5 ~ 1 ﹪, culture temperature be 37 DEG C, shaking flask rotating speed cultivates 20h under being 180rpm; By bacstearothermophilus strain inoculation good for activation culture to being equipped with in bio-reactor that volume content is 50 ﹪ substratum-2, the volume inoculum size of bacterial classification is 1 ﹪, culture temperature be 50 DEG C, air flow 8L/s, bio-reactor mixing speed cultivate 20h under being 250rpm, bacstearothermophilus content>=10 in tank 9individual/ml;
Described activation medium-3 is filled a prescription as yeast extract paste extractum carnis 3 g/l, soy peptone 10 g/l, agar 16 g/l;
Described substratum-2 is filled a prescription as dregs of beans 5 g/l, yeast extract paste 8 g/l, K2HP04 2 g/l.
Embodiment 2-1
(1) get chicken corpse 100 kg that dies of illness, pulverized by pulverizer.
(2) close the preparation of microbiobacterial agent, process is as follows:
Subtilis-1(ZJNB0001 after Example 1-1 cultivates), subtilis-2 (ZJHZ0005) after embodiment 1-2 cultivates, Bacillus licheniformis (ZJHZ1025) after embodiment 1-3 cultivates, each 5 ml of bacstearothermophilus (ZJNB0008) 5 kinds of bacterium liquid after bacillus cereus (ZJWZ0002) after embodiment 1-4 cultivates and embodiment 1-5 cultivate carry out bacterial count, then in careless genus bacillus-1: subtilis-2: Bacillus licheniformis: bacillus cereus: the number ratio=3:2:2:2:1 ratio of bacstearothermophilus is mixed into the complex micro organism fungicide of 30 L.
(3) the chicken corpse (100g) of dying of illness crushed is mixed with 30 L complex micro organism fungicides, then chicken manure (6 kg), mud (17kg), stalk (12kg) mixture of 35 kg is added, fermentation 2 d is carried out at putting into fermentor tank 57 DEG C after abundant mixing, fermenting process carries out tipping operation, air flow 0.9VVM, tank pressure keeps 0.04Mpa.The fertilizer getting 80 ﹪ after having fermented makes biological organic fertilizer, and the substrate of remaining 20 ﹪ can utilize again.
Embodiment 2-2
(1) get sick dead pig corpse 200 kg, pulverized by pulverizer.
(2) close the preparation of microbiobacterial agent, process is as follows:
Subtilis-1(ZJNB0001 after Example 1-1 cultivates), subtilis-2 (ZJHZ0005) after embodiment 1-2 cultivates, Bacillus licheniformis (ZJHZ1025) after embodiment 1-3 cultivates, each 5 ml of bacstearothermophilus (ZJNB0008) 5 kinds of bacterium liquid after bacillus cereus (ZJWZ0002) after embodiment 1-4 cultivates and embodiment 1-5 cultivate carry out bacterial count, then in careless genus bacillus 1: subtilis 2: Bacillus licheniformis: bacillus cereus: bacstearothermophilus=3:2:2:2:1 ratio is mixed into the complex micro organism fungicide of 60 L.
(3) the sick dead pig corpse (200g) crushed is mixed with complex micro organism fungicide (60 L), then the chicken manure (12 kg) of 70 kg, mud (34 kg), stalk (24 kg) mixture is added, put into fermentor tank after abundant mixing to ferment, fermenting process carries out tipping operation, air flow 0.9VVM, tank pressure keeps 0.04Mpa, and leavening temperature is 57 DEG C, and ferment 2 d.The fertilizer getting 80 ﹪ after having fermented makes biological organic fertilizer, and the substrate of remaining 20 ﹪ can utilize again.
 
Embodiment 2-3
(1) get duck corpse 100 kg that dies of illness, pulverized by pulverizer.
(2) close the preparation of microbiobacterial agent, process is as follows:
Subtilis-1(ZJNB0001 after Example 1-1 cultivates), subtilis-2 (ZJHZ0005) after embodiment 1-2 cultivates, Bacillus licheniformis (ZJHZ1025) after embodiment 1-3 cultivates, each 5 ml of bacstearothermophilus (ZJNB0008) 5 kinds of bacterium liquid after bacillus cereus (ZJWZ0002) after embodiment 1-4 cultivates and embodiment 1-5 cultivate carry out bacterial count, then in careless genus bacillus 1: subtilis 2: Bacillus licheniformis: bacillus cereus: bacstearothermophilus=3:2:2:2:1 ratio is mixed into the complex micro organism fungicide of 30 L.
(3) the duck corpse (100g) of dying of illness crushed is mixed with complex micro organism fungicide (30 L), then chicken manure (6 kg), mud (17kg), stalk (12kg) mixture of 35 kg is added, put into fermentor tank after abundant mixing to ferment, fermenting process carries out tipping operation, air flow 0.9VVM, tank pressure keeps 0.04Mpa, and leavening temperature is 57 DEG C, fermentation 2d.The fertilizer getting 80 ﹪ after having fermented makes biological organic fertilizer, and the substrate of remaining 20 ﹪ can utilize again.
Embodiment 2-4
(1) get duck corpse 100 kg that dies of illness, pulverized by pulverizer.
(2) close the preparation of microbiobacterial agent, process is as follows:
Subtilis-1(ZJNB0001 after Example 1-1 cultivates), subtilis-2 (ZJHZ0005) after embodiment 1-2 cultivates, Bacillus licheniformis (ZJHZ1025) after embodiment 1-3 cultivates, each 5 ml of bacstearothermophilus (ZJNB0008) 5 kinds of bacterium liquid after bacillus cereus (ZJWZ0002) after embodiment 1-4 cultivates and embodiment 1-5 cultivate carry out bacterial count, then in careless genus bacillus 1: subtilis 2: Bacillus licheniformis: bacillus cereus: bacstearothermophilus=3:2:2:2:1 ratio is mixed into the complex micro organism fungicide of 45 L.
(3) the duck corpse (100g) of dying of illness crushed is mixed with complex micro organism fungicide (45L), then chicken manure (3 kg), mud (16.5kg), stalk (10.5kg) mixture of 30kg is added, put into fermentor tank after abundant mixing to ferment, fermenting process carries out tipping operation, air flow 0.8VVM, tank pressure keeps 0.05Mpa, and leavening temperature is 55 DEG C, fermentation 3d.The fertilizer getting 80 ﹪ after having fermented makes biological organic fertilizer, and the substrate of remaining 20 ﹪ can utilize again.
Embodiment 2-5
(1) get duck corpse 100 kg that dies of illness, pulverized by pulverizer.
(2) close the preparation of microbiobacterial agent, process is as follows:
Subtilis-1(ZJNB0001 after Example 1-1 cultivates), subtilis-2 (ZJHZ0005) after embodiment 1-2 cultivates, Bacillus licheniformis (ZJHZ1025) after embodiment 1-3 cultivates, each 5 ml of bacstearothermophilus (ZJNB0008) 5 kinds of bacterium liquid after bacillus cereus (ZJWZ0002) after embodiment 1-4 cultivates and embodiment 1-5 cultivate carry out bacterial count, then in careless genus bacillus 1: subtilis 2: Bacillus licheniformis: bacillus cereus: bacstearothermophilus=3:2:2:2:1 ratio is mixed into the complex micro organism fungicide of 40L.
(3) the duck corpse (100g) of dying of illness crushed is mixed with complex micro organism fungicide (40L), then the chicken manure (10kg) of 40kg, mud (16kg), stalk (14kg) mixture is added, put into fermentor tank after abundant mixing to ferment, fermenting process carries out tipping operation, air flow 1.0VVM, tank pressure keeps 0.03Mpa, and leavening temperature is 62 DEG C, fermentation 2d.The fertilizer getting 80 ﹪ after having fermented makes biological organic fertilizer, and the substrate of remaining 20 ﹪ can utilize again.
Embodiment 2-6
(1) get duck corpse 100 kg that dies of illness, pulverized by pulverizer.
(2) close the preparation of microbiobacterial agent, process is as follows:
Subtilis-1(ZJNB0001 after Example 1-1 cultivates), subtilis-2 (ZJHZ0005) after embodiment 1-2 cultivates, Bacillus licheniformis (ZJHZ1025) after embodiment 1-3 cultivates, each 5 ml of bacstearothermophilus (ZJNB0008) 5 kinds of bacterium liquid after bacillus cereus (ZJWZ0002) after embodiment 1-4 cultivates and embodiment 1-5 cultivate carry out bacterial count, then in careless genus bacillus 1: subtilis 2: Bacillus licheniformis: bacillus cereus: bacstearothermophilus=3:2:2:2:1 ratio is mixed into the complex micro organism fungicide of 40L.
(3) the duck corpse (100g) of dying of illness crushed is mixed with complex micro organism fungicide (40L), then the chicken manure (10kg) of 40kg, mud (22kg), stalk (8kg) mixture is added, put into fermentor tank after abundant mixing to ferment, fermenting process carries out tipping operation, air flow 1.0VVM, tank pressure keeps 0.04Mpa, and leavening temperature is 60 DEG C, fermentation 3d.The fertilizer getting 80 ﹪ after having fermented makes biological organic fertilizer, and the substrate of remaining 20 ﹪ can utilize again.
Above-described embodiment is not that the present invention is not limited only to above-described embodiment for restriction of the present invention, as long as meet application claims, all belongs to protection scope of the present invention.

Claims (5)

1. utilize biological degradation dead livestock and poultry to prepare a method for organic fertilizer, it is characterized in that the method comprises the following steps:
Step (1). dead livestock and poultry corpse is pulverized by pulverizer, then fully mixes with the weightmeasurement ratio of complex micro organism fungicide by 100 Kg:30 ~ 45 L, obtain compound;
Described complex micro organism fungicide is by cultivating rear subtilis-1 ZJNB0001, subtilis-2 ZJHZ0005 after cultivating, Bacillus licheniformis ZJHZ1025 after cultivating, after cultivating, bacillus cereus ZJWZ0002 and the rear bacstearothermophilus ZJNB0008 of cultivation forms, subtilis-1 ZJNB0001 after wherein cultivating, subtilis-2 ZJHZ0005 after cultivating, Bacillus licheniformis ZJHZ1025 after cultivating, after cultivating, bacillus cereus ZJWZ0002 is 3:2:2:2:1 with the number ratio of the rear bacstearothermophilus ZJNB0008 of cultivation,
Step (2). put into fermentor tank after fully being mixed with chicken manure, mud, stalk by the compound that step (1) obtains and ferment, fermenting process carries out tipping operation, air flow 0.8 ~ 1.0 VVM, tank pressure keeps 0.03 ~ 0.05 Mpa, leavening temperature remains on 55 ~ 62 DEG C, and the cycle is 2 ~ 3d; Ferment and obtained biological organic fertilizer;
The total add-on of described chicken manure, mud, stalk is 30 ~ 40 ﹪ of the compound weight that step (1) obtains; In chicken manure, mud, stalk, the mass content of chicken manure is 10 ~ 25 ﹪, and the mass content of mud is 40 ~ 55 ﹪, and the mass content of stalk is 20 ~ 35 ﹪.
2. a kind of method utilizing biological degradation dead livestock and poultry to prepare organic fertilizer as claimed in claim 1, it is characterized in that the cultural method of described subtilis-1 ZJNB0001, subtilis-2 ZJHZ0005 is as follows: being seeded to by the Bacillus subtilis strain cultivated at purifying and volume content is housed is in the shaking flask of 15 ﹪ activation mediums-1, the volume inoculum size of bacterial classification is 0.5 ~ 1 ﹪, culture temperature be 37 DEG C, shaking flask rotating speed cultivates 20h under being 180rpm; The Bacillus subtilis strain that activation culture is good is inoculated into and is equipped with in bio-reactor that volume content is 50 ﹪ substratum-1, the volume inoculum size of bacterial classification is 1 ﹪, culture temperature be 37 DEG C, air flow 8L/s, bio-reactor mixing speed cultivate 20h under being 220rpm, bacillus subtilis bacterial content>=10 in tank 9individual/ml;
The formula of described activation medium-1 is glucose 18g/l, peptone 12g/l, sodium-chlor 3g/l and extractum carnis 0.2g/l;
Described substratum-1 is filled a prescription as Semen Maydis powder 10g/l, glucose 3g/l, soybean cake powder 18g/l, fish meal 3g/l, calcium carbonate 5g/l, ammonium sulfate 0.8g/l, dipotassium hydrogen phosphate 0.1g/l, magnesium sulfate 0.1g/l and manganous sulfate 0.1g/l.
3. a kind of method utilizing biological degradation dead livestock and poultry to prepare organic fertilizer as claimed in claim 1, it is characterized in that the cultural method of described Bacillus licheniformis ZJHZ1025 is as follows: the Bacillus licheniformis ZJHZ1025 strain inoculation of being cultivated by purifying is in the shaking flask of 15 ﹪ activation mediums-2 to being equipped with volume content, the volume inoculum size of bacterial classification is 0.5 ~ 1 ﹪, culture temperature be 37 DEG C, shaking flask rotating speed cultivates 20h under being 180rpm; By Bacillus licheniformis ZJHZ1025 strain inoculation good for activation culture to being equipped with in bio-reactor that volume content is 50 ﹪ fermention mediums, the volume inoculum size of bacterial classification is 1 ﹪, culture temperature be 37 DEG C, air flow 8L/s, bio-reactor mixing speed cultivate 20h under being 200rpm, Bacillus licheniformis ZJHZ1025 content>=10 in tank 9individual/ml;
The formula of described activation medium-2 is peptone 8g/l, extractum carnis 1g/l and sodium-chlor 3g/l;
Described fermentative medium formula is soybean cake powder 13g/l, Semen Maydis powder 13g/l, dipotassium hydrogen phosphate 0.05g/l, potassium primary phosphate 0.05g/l and calcium chloride 2g/l.
4. a kind of method utilizing biological degradation dead livestock and poultry to prepare organic fertilizer as claimed in claim 1, it is characterized in that described bacillus cereus ZJWZ0002 cultural method is as follows: the bacillus cereus ZJWZ0002 strain inoculation of being cultivated by purifying is in the shaking flask of 15 ﹪ activation mediums-3 to being equipped with volume content, the volume inoculum size of bacterial classification is 0.5 ~ 1 ﹪, be 37 DEG C in culture temperature, shaking flask rotating speed is cultivate 20h under 180rpm; By bacillus cereus ZJWZ0002 strain inoculation good for activation culture to being equipped with in bio-reactor that volume content is 50 ﹪ substratum-2, the volume inoculum size of bacterial classification is 1 ﹪, culture temperature be 37 DEG C, air flow 8L/s, bio-reactor mixing speed cultivate 20h under being 250rpm, bacillus cereus ZJWZ0002 content>=10 in tank 9individual/ml;
Described activation medium-3 is filled a prescription as yeast extract paste 5g/l, peptone 10g/l, NaCl 5g/l;
Described substratum-2 is filled a prescription as glucose 10 g/l, peptone 5g/l, yeast extract paste 8g/l, NaCl 5g/l, K 2hP0 47g/l, CaCl 22.5g/l, MgS0 41g/l, MnS0 40.01g/l.
5. a kind of method utilizing biological degradation dead livestock and poultry to prepare organic fertilizer as claimed in claim 1, it is characterized in that described bacstearothermophilus ZJNB0008 cultural method: the bacstearothermophilus ZJNB0008 strain inoculation of being cultivated by purifying is in the shaking flask of the activation medium-3 of 15 ﹪ to being equipped with volume content, the volume inoculum size of bacterial classification is 0.5 ~ 1 ﹪, culture temperature be 37 DEG C, shaking flask rotating speed cultivates 20h under being 180rpm; By bacstearothermophilus ZJNB0008 strain inoculation good for activation culture to being equipped with in bio-reactor that volume content is 50 ﹪ substratum-2, the volume inoculum size of bacterial classification is 1 ﹪, culture temperature be 50 DEG C, air flow 8L/s, bio-reactor mixing speed cultivate 20h under being 250rpm, bacstearothermophilus ZJNB0008 content>=10 in tank 9individual/ml;
Described activation medium-3 is filled a prescription as yeast extract paste extractum carnis 3g/l, soy peptone 10g/l, agar 16g/l;
Described substratum-2 is filled a prescription as dregs of beans 5g/l, yeast extract paste 8g/l, K 2hP0 42g/l.
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CN104894007A (en) * 2015-05-12 2015-09-09 安徽农业大学 Compound microorganism preparation, preparing method and application of compound microorganism preparation to processing animal carcasses
CN104894007B (en) * 2015-05-12 2019-07-05 安徽全民环保科技有限公司 Complex microorganism preparations and preparation method and the application in processing animal carcass
CN104926447A (en) * 2015-06-05 2015-09-23 扬州市水产生产技术指导站 Preparation method and application of bio-organic fertilizer for cultivation of beneficial algas in ponds
CN104987144A (en) * 2015-07-15 2015-10-21 广西宏华生物实业股份有限公司 Organic rice base fertilizer and preparing method of organic rice base fertilizer
CN105199980A (en) * 2015-09-10 2015-12-30 山东华牧天元农牧股份有限公司 Compounded microbial preparation and method for innocent treatment of animal carcasses
CN105294178A (en) * 2015-10-09 2016-02-03 南京元凯生物能源环保工程有限公司 Method for biodegrading animal carcass by utilizing biogas residues
CN105272455A (en) * 2015-10-13 2016-01-27 山西晋东丰裕肥业有限公司 Biological organic fertilizer rich in animal protein and production method thereof
CN109234349A (en) * 2015-12-30 2019-01-18 中林山水(北京)生态科技股份有限公司 Microbial inoculum and method for waste biomass body recycling treatment after fowl poultry kind animal dead
CN105457989A (en) * 2015-12-30 2016-04-06 中林山水(北京)生态科技股份有限公司 Resourceful treatment method for waste bodies of dead livestock and poultry animals
CN105541420A (en) * 2016-01-19 2016-05-04 安徽工业大学 Continuous aerobic composting device and disposal method for dead livestock and poultry on livestock and poultry farm
CN105541420B (en) * 2016-01-19 2020-04-14 安徽工业大学 Dead livestock and poultry continuous aerobic composting device in livestock and poultry farm and treatment method thereof
CN106365788A (en) * 2016-08-25 2017-02-01 南京国龙生物科技有限公司 Harmless microorganism treatment method for cadavers of livestock and poultry
CN108164367A (en) * 2018-02-05 2018-06-15 大连理工大学 Fast and harmless handles the biological method of dead livestock and poultry
CN108752065A (en) * 2018-06-13 2018-11-06 郝丽华 A kind of method of microbial fermentation processing animals died of illness
CN108675839A (en) * 2018-07-06 2018-10-19 湖南泰谷生态工程有限公司 A kind of recovery processing compost method for the fowl that dies of illness
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