CN104304115A - Genetic improvement method of growth rate of rainbow trout albino mutant strain - Google Patents

Genetic improvement method of growth rate of rainbow trout albino mutant strain Download PDF

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Publication number
CN104304115A
CN104304115A CN201410557859.2A CN201410557859A CN104304115A CN 104304115 A CN104304115 A CN 104304115A CN 201410557859 A CN201410557859 A CN 201410557859A CN 104304115 A CN104304115 A CN 104304115A
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rainbow trout
albino
growth rate
trout
genetic improvement
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CN104304115B (en
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户国
王炳谦
谷伟
孙鹏
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Heilongjiang Institute of Fisheries Chinese Academy of Fishery Sciences
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Heilongjiang Institute of Fisheries Chinese Academy of Fishery Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention relates to a genetic improvement method of a growth rate of a rainbow trout albino mutant strain. The invention aims to improve the growth performance of the rainbow trout albino mutant strain by introducing the blood of a rapid-growth wide type rainbow trout superior strain under the condition of unchanging the body color, the eyeball color and other characteristics. The method comprises the following steps: (1) hybridizing wide type female rainbow trout with no direct relation with male albino mutant rainbow trout with no direct relation to generate fry of F1 generation; (2) selecting individuals with yellow body color and black eyeball color to carry out individual homogeneous mating; (3) for the albino rainbow trout with the red eyeball color and yellow body color in F2 generation, selecting the albino rainbow trout with the greatest body mass and full-sib individuals to carry out male and female mating, and reserving the offspring of the albino rainbow trout with the red eyeball color and yellow body color. The genetic improvement method provided by the invention has the advantages of increasing the degree of genetic diversity in populations, reducing the degree of inbreeding, and significantly improving the growth rate of the albino trout.

Description

A kind of genetic improvement method of rainbow trout albino mutation strain growth rate
Technical field
The invention belongs to aquatic livestock genetic breeding field, particularly relate to the genetic improvement method of rainbow trout albino mutation strain growth rate.
Background technology
The wild rainbow trout of natural world (Oncorhyncus mykiss), body colour is cinerous, and eyeball look is black, and its albino mutant (albino Oncorhyncus mykiss) body colour is light yellow, and eyeball look is pink.Academia it is generally acknowledged, rainbow trout albino mutant is similar to other mammiferous albinos, is that wild type genes of individuals is suddenlyd change in the chromatophore that causes and lacked melanic albino.Cultivation rainbow trout albino mutation strain is the earliest that Tokyo aquatic products university cultivates acquisition, and introduced a fine variety China by Heilongjiang Inst. of Aquatic Products, Chinese Academy of Aquatic Products Scie in 1996, due to its yellow body colour, in Chinese extension process, be called as yellow rainbow trout, day capital trout or golden trout Japanese strain.Rainbow trout is mainly sold with the form of fillet in Japan and American-European countries, and body colour proterties does not possess obvious economic worth.China eating habit be whole piece fish processing and eating, and culture on gold for expensive, dinner party entree take fish as honor.Rainbow trout albino mutant is with delicious meat, and entire body is bordering on golden yellow, deeply likes having higher consumption demand by the people.
Through test cross experiment and other genetic analysis, the gene pairs wild body colour gene of known control yellow is dominant, control the wild eyeball color base of gene pairs of blood-shot eye illness ball look because recessive, confirm that the albino look genotype of albino rainbow trout belongs to dominant homogeneous, and red eyeball look genotype is that recessiveness is isozygotied; The heredity of body colour and eyeball look meets mendelian inheritance.Because day, capital trout was breeding material based on the mutant of wild type body colour, fix the strain of acquisition through traversed by, so this strain inbreeding level is higher, population genetic diversity is lower, after long-term conservation maintains, growth rate is more a lot of slowly than the wild type rainbow trout growth speed through hereditary and selection.
Summary of the invention
Under the object of the invention is to provide a kind of condition not changing the characteristic trait such as body colour and eyeball look, utilize the blood lineage introducing growth wild type rainbow trout excellent strain fast, the method for improvement rainbow trout albino mutation strain growth performance.Meanwhile, the present invention also aims to a kind of genetic improvement method that rainbow trout albino mutation strain growth performance is provided.
The genetic improvement method of a kind of rainbow trout albino mutation strain growth rate of the present invention, it carries out according to following steps:
One, using the wild type rainbow trout raun without direct affiliation as female parent, using without direct affiliation pure lines day capital trout albino mutant milter as male parent;
Two, female parent and male parent are hybridized, obtain F1 generation seed;
Three, yellow black eye ball look individuality of selecting and remain in F1 generation seed is avoided mating like to like is carried out on the basis of sib mating, obtains F2 generation individual;
Four, float to newly hatched larvae after 30 ~ 35 days for individual incubating oosperm at F2, swim is raised respectively according to family in independent seed selection cylinder;
Five, select the albino rainbow trout healthy individuals with blood-shot eye illness ball look yellow, anaesthetize, carry out electronic marker;
Six, the rainbow trout individuality after mark is placed in seed selection cylinder and carries out cultivation observation;
Seven, cement pit is put into all individualities in step 6 in seed selection cylinder after foster 1 week temporarily, mix same pond and raise to 24 monthly ages mensuration weights;
Eight, select the maximum albino rainbow trout of Individual Quality and full sibs individuality to carry out male and female mating, retain and produce the albino rainbow trout that offspring is all blood-shot eye illness ball look yellow, namely obtain the rainbow trout albino mutation strain with quick growth characteristics.
The present invention comprises following beneficial effect:
Present invention employs a kind of breeding method of rainbow trout albino mutation strain of quick growth, albino look genotype belongs to dominant homogeneous, and red eyeball look genotype is that recessiveness is isozygotied.Control yellow gene loci and be expressed as (Yellow, Y), control blood-shot eye illness ball look gene loci and be expressed as (Red, R), dominant allele letter capitalization, Recessive alleles lower-case letters, then the genotype of wild type rainbow trout (body cinerous pupil look) can be expressed as (yyRR), and the genotype of albino mutant (the furious look of body yellow) is expressed as (YYrr).
The present invention introduces the blood lineage of wild type rainbow trout breeding in day capital trout, can increase population genetic diversity degree, reduces inbreeding level, significantly improves albefaction rainbow trout growth speed.
By the rainbow trout that method of the present invention is cultivated, by to its growth performance contrast test, result shows that new lines does not change at albino rainbow trout typical appearance such as yellow, blood-shot eye illness ball looks, 24 monthly age body mass ratio wild type rainbow trout breeding populations are lower slightly, but be significantly higher than a day capital trout conservation colony, growth rate improves 39.31% than day capital trout conservation colony.New lines expands in numerous popularization at albino rainbow trout breeding and uses.
Embodiment
Embodiment one: the genetic improvement method of a kind of rainbow trout albino mutation strain growth rate of present embodiment, it carries out according to following steps:
One, using the wild type rainbow trout raun without direct affiliation as female parent, using without direct affiliation pure lines day capital trout albino mutant milter as male parent;
Two, at short-day, when water temperature is at 10 ~ 11 DEG C, rainbow trout enters breeding period, female parent and male parent is carried out hybridization combo separately according to 1:1, obtains F1 generation seed;
(1) parent population maturity is differentiated
1, female parent population: belly expands and soft, the red and swollen evagination of gonopore;
2, male parent population: nuptial coloration is more bright-coloured, and belly does not significantly expand, light pressure belly has seminal fluid to flow out;
(2) artificial propagation
1, anaesthetize
By male and female parent population respectively with Phenoxyethanol anesthesia, dosage is 0.5ml/L, notes observing anesthesia, when parent population belly upward or be in quiescent condition, namely show the narcosis that fish has reached suitable, should in time parent population be shifted out from anesthesia liquid, prevent deep anaesthesia from causing parent population dead;
2, gamete obtains
First, gather milter seminal fluid, after collection, check sperm viability, select lucifuge 4 DEG C of stored refrigerated that sperm viability is good to place, note when collecting semen, first the water dry towel of fish body surface is dried, especially cloacal aperture place, the seminal fluid that extraction comes is avoided illumination and is had water to be splashed into; Ovum artificial collection is carried out after having gathered sperm, according to the semen dosage gathered before and actual output ovum quantity, gather how many rauns according to the ratio-dependent of 1:1, when gathering ovum, fish body surface also will be kept anhydrous, the ovum lucifuge be collected, and water can not be had to enter; The ovum 0.9-1.0%NaCl aqueous solution in each basin cleans repeatedly, and NaCl solution slowly will be poured into along tub wall, pour out, and the damaged ovum in fish-egg, coelomic fluid, ight soil is cleaned;
3, artificial insemination
1) if conditions permit can use wet method to be fertilized, namely mass percentage is selected to be that 0.9 ~ 1.0%NaCl aqueous solution carries out fertilization activity as medium, first fish-egg is originated in the 0.9-1.0%NaCl aqueous solution, liquid level has just flooded fish-egg the best, afterwards the male of the seminal fluid be collected in advance is individually fertilized with raun according to 1:1, whole fertilization process all wants lucifuge to carry out, static 3 minutes of after fertilization, then fertilized egg is clean with clear water wash-out, then place and just can hatch for about 40 minutes;
2) if condition does not allow, dry method also can be adopted to be fertilized, namely directly fish-egg to be originated in little container, afterwards the seminal fluid be collected in advance is individually fertilized in right amount according to 1:1; Whole fertilization process all wants lucifuge to carry out, static 3 minutes of after fertilization, then that fertilized egg is clean with clear water wash-out, then places and just can hatch for about 40 minutes;
(3), incubating oosperm
1, hatch in lucifuge situation.Fertilized egg after abundant imbibition will be placed in vertical incubator incubation plate (or independent hatching pail) in units of family hatches, by placement about 3000 fertilized eggs in each incubation plate (in bucket);
2, water temperature is hatched: 9 ~ 11 DEG C;
3, Spawning water: spring or well water, draining dissolved oxygen remains on more than 6mg/L; At least check two times inflows every day, prevent tap from blocking;
4, every day is with 8 × 10 -4ml/L hydrogenperoxide steam generator soaks 30 minutes, prevents water mo(u)ld from growing;
5, every day, 8:00,14:00 recorded water temperature;
(4) eyed eggs management
1, rainbow trout fertilized egg sends out eye when accumulative temperature reaches about 160 DEG C of d (subsisting) left and right in 10 DEG C of hatching water temperatures, after fish-egg sends out eye, oxygen consumption sharply rises, and stimulates relatively stable to external world, in time eyed eggs should be moved into from hatching pail and place side by side in groove, and sort out dead ovum in time;
2, manually picking ovum: pick ovum dish and put into water, tiling last layer eyed eggs, sorting out dead ovum with picking ovum folder; If dead ovum is more, 10% (proportion 1.1) salt solution can be prepared, after being pulled out by the dead ovum swimming in upper strata, more manually pick ovum;
Three, yellow black eye ball look individuality of selecting and remain in F1 generation seed is avoided mating like to like is carried out on the basis of sib mating, obtains F2 generation individual;
Four, float to newly hatched larvae after about 30 ~ 35 days for individual incubating oosperm at F2, hatching m is consistent with method described in step 2, is raised respectively by swim according to family in independent seed selection cylinder;
Five, select the albino rainbow trout with blood-shot eye illness ball look yellow individual, anaesthetize, carry out electronic marker;
Six, the rainbow trout individuality after mark is placed in seed selection cylinder and carries out cultivation observation;
Seven, cement pit is put into all individualities in step 6 in seed selection cylinder after foster 1 week temporarily, mix same pond and raise to 24 monthly ages mensuration weights;
Eight, select the maximum albino rainbow trout of Individual Quality and full sibs individuality to carry out male and female mating, retain and produce the albino rainbow trout that offspring is all blood-shot eye illness ball look yellow, namely obtain the rainbow trout albino mutation strain with quick growth characteristics.
The wild type rainbow trout raun without direct affiliation of the present embodiment, the albino mutant milter without direct affiliation and pure lines day capital trout are all commercially available prod.
Embodiment two: present embodiment and embodiment one unlike: in the F2 generation described in step 4, the individual independent seed selection cylinder at diameter 0.8m × high 0.6m × dark 0.3m was raised to 50g.Other is identical with embodiment one.
Embodiment three: present embodiment and embodiment one or two unlike: select in step 5 and there is the albino rainbow trout healthy individuals of seeing red ball look yellow refer to: grow to Individual Quality and reach 50g, the individuality with the albino rainbow trout of blood-shot eye illness ball look yellow of long more than the 16cm of body.Other is identical with embodiment one or two.
Embodiment four: one of present embodiment and embodiment one to three unlike: carrying out in step 5 anaesthetizing is adopt concentration to be that the Phenoxyethanol solution of 0.5mL/L carries out.Other is identical with one of embodiment one to three.
Embodiment five: one of present embodiment and embodiment one to four unlike: the water temperature carrying out electronic marker in step 5 is more than 12 DEG C.Other is identical with one of embodiment one to four.
Embodiment six: one of present embodiment and embodiment one to five unlike: carry out in step 5 before electronic marker with mark after to injection site disinfection, concrete conditions for sterilization is: disinfecting drug selects volumn concentration to be the ethanolic solution of 75% ~ 80%, chip, injection needle volumn concentration is 75% alcohol immersion sterilization.Other is identical with one of embodiment one to five.
Embodiment seven: one of present embodiment and embodiment one to six unlike: the injection site carrying out electronic marker in step 5 is by abdomeinal fin leading edge 1.5cm, syringe needle and fish body belly are 45° angle, oblique cutting enters intrathecal, is then the parallel chip being pushed forward into electronic marker with fish length axle.Other is identical with one of embodiment one to six.
Embodiment eight: one of present embodiment and embodiment one to seven unlike: step step 2 in female parent and male parent are hybridized, obtain F1 generation seed be short-day, water temperature be 10 ~ 11 DEG C, under rainbow trout enters the condition of breeding period, female parent and male parent are carried out hybridization combo separately according to 1:1 ratio, obtains F1 generation seed.Other is identical with one of embodiment one to seven.
Below in conjunction with specific embodiment, the present invention is described in detail.Following embodiment is of the present invention illustrating; the mentioned concrete kind as male parent and maternal rainbow trout in embodiment; and specifically geographical population, marketable fish or breeding population are not limitation of the invention, what the present invention protected is also not limited to listed by embodiment.
Due to dominant inheritance, recessive inheritance, Mendelian inheritance, the mating of full sibs male and female, traversed by fix, genetic improvement, systematic breeding, families selecting and individual choice, the noun such as PIT (passive integrated transponder) electronic marker be all the term commonly used of those skilled in the art or breeding method, be not therefore described in detail at this and explain.
Embodiment 1
Have a breeding method for the rainbow trout albino mutation strain of quick growth characteristics, step is followed successively by:
Step 1,
The rainbow trout excellent strain using Heilongjiang Inst. of Aquatic Products, Chinese Academy of Aquatic Products Scie to cultivate is wild type rainbow trout (yyRR), as female parent; Albino mutant is derived from the day capital trout conservation colony that 1996 introduce China, as male parent (YYrr).It is cold water fish experiment station, the Bohai Sea of Heilongjiang Inst. of Aquatic Products, Chinese Academy of Aquatic Products Scie that place is implemented in test.The germplasm materials related in the present embodiment is all stored in the Ministry of Agriculture's cold water fishes genetic breeding center being positioned at cold water fish experiment station, the Bohai Sea of Heilongjiang Inst. of Aquatic Products, Chinese Academy of Aquatic Products Scie, and provides germ plasm resource to Society Open.
Because albino look genotype belongs to dominant homogeneous, and red eyeball look genotype is that recessiveness is isozygotied, so wild type rainbow trout and albino rainbow trout are the yellow pupil look of body more than more than 90% offspring's phenotype.Hybridize with wild type rainbow trout (yyRR) the 20 tail raun without direct affiliation and albino mutant (YYrr) the 20 tail milter without direct affiliation, production F1 generation hybridization family full-sibs 20, each family 200 tail seed, the all idiotypes of F1 generation are (YyRr), all familys are all without the need to discriminate individuals, each family is raised respectively to 24 monthly ages and is measured weights, each family select and remain respectively the weight sequence raun of first 3 and milter for subsequent use;
Step 2,
1) yellow black eye ball look individuality of selecting and remain in F1 generation is avoided mating like to like is carried out on the basis of sib mating, and produce F2 for rainbow trout family full-sibs 30, it is individual that each family retains 400 tails at random;
2) absorb at yolk sac, after prelarva floats, (about 30 ~ 35 days) are cultivated respectively according to family and are raised to 50g at the independent seed selection cylinder of diameter 0.8m × high 0.6m × dark 0.3m;
3) grow to weight and reach 50g, the healthy individuals with the albino rainbow trout of blood-shot eye illness ball look yellow of long more than the 16cm of body, adopt Phenoxyethanol solution (0.5ml/L) anesthesia, implant PIT electronic marker (ID-100 type, Trovan company of Britain).For preventing fish body wound to be difficult to healing, water temperature during mark will be strict controlled in more than 12 DEG C;
4) personnel placement of PIT electronic marker implant procedure: operational label rifle 1 people, operation scanner 1 people, fish body fixes 1 people, and fish body anaesthetizes 1 people, experimental record person 1 people; Carry out strict disinfectant program to injection site before mark with after mark, disinfecting drug selects the ethanolic solution of 75% ~ 80%; Chip, entry needle first-class use 75% alcohol immersion is sterilized;
5) injection site is by abdomeinal fin leading edge 1.5cm, and syringe needle and fish body belly are 45° angle, and oblique cutting enters intrathecal, then with fish length axle is parallelly to be pushed forward into chip;
6) mark position to observe through carrying out cultivation containing the circular seed selection cylinder putting into diameter 0.8m × high 0.6m × dark 0.3m after 75% ethanol cotton balls wiping, after fish body tag, in seed selection cylinder, foster 1 Zhou Houzai puts into cement pit raising temporarily;
7), after implanting PIT electronic marker, all individuality mixing are raised to 24 monthly ages mensuration weights with pond;
Step 3,
To in step 2 7) Individual Quality measure in maximum albino rainbow trout 30 tail carry out male and female mating respectively with full sibs individuality, retain and produce the albino rainbow trout that offspring is all blood-shot eye illness ball look yellow, namely obtain the rainbow trout albino mutation strain with quick growth characteristics.
Growth performance test is carried out to the rainbow trout albino mutation strain that embodiment 1 obtains:
The experiment of growth performance contrast test performs in cold water fish experiment station, the Bohai Sea of Heilongjiang Inst. of Aquatic Products, Chinese Academy of Aquatic Products Scie, and position is seated near Jingpo Lake (north latitude 44.02 °, east longitude 128.74 °); Feeding and management is carried out according to SalmonFood company Vitacare feedstuff feeding guidelines.Test uses the depth of water 1.2 meters, long 30 meters, the perennial draingage cement pond clearing of wide 5 meters, and water source is natural fountain water, and the anniversary, water temperature excursion was at 5.0 ~ 18.0 DEG C, flow 20 ~ 30L/s, partial pressure of oxygen 7 ~ 11mg/L; By rainbow trout excellent strain G3 breeding population 1000 high-quality eyed eggses from generation to generation, albino rainbow trout strain 1000 high-quality eyed eggses after genetic improvement, day capital trout strain conservation colony 1000 high-quality eyed eggses to perform in steps 2 2) to 6) after step, each colony random selecting 500 tail healthy seedling is raised together with to 24 monthly ages with pond, measure weight, relatively growth performance, the results are shown in Table 1.
Growth performance contrast test result shows, new lines does not change at albino rainbow trout typical appearance such as yellow, blood-shot eye illness ball looks, 24 monthly age body mass ratio wild type rainbow trout breeding populations are lower slightly, but be significantly higher than a day capital trout conservation colony, growth rate improves 39.31% than day capital trout conservation colony.New lines expands in numerous popularization at albino rainbow trout breeding and uses.
The growth performance contrast test of albino rainbow trout and conservation albino rainbow trout improved by table 1
* different letter representation significant difference (P<0.05) is marked in same column.

Claims (8)

1. a genetic improvement method for rainbow trout albino mutation strain growth rate, is characterized in that it carries out according to following steps:
One, using the wild type rainbow trout raun without direct affiliation as female parent, using without direct affiliation pure lines day capital trout albino mutant milter as male parent;
Two, female parent and male parent are hybridized, obtain F1 generation seed;
Three, yellow black eye ball look individuality of selecting and remain in F1 generation seed is avoided mating like to like is carried out on the basis of sib mating, obtains F2 generation individual;
Four, float to newly hatched larvae after 30 ~ 35 days for individual incubating oosperm at F2, swim is raised respectively according to family in independent seed selection cylinder;
Five, select the albino rainbow trout healthy individuals with blood-shot eye illness ball look yellow, anaesthetize, carry out electronic marker;
Six, the rainbow trout individuality after mark is placed in seed selection cylinder and carries out cultivation observation;
Seven, cement pit is put into all individualities in step 6 in seed selection cylinder after foster 1 week temporarily, mix same pond and raise to 24 monthly ages mensuration weights;
Eight, select the maximum albino rainbow trout of Individual Quality and full sibs individuality to carry out male and female mating, retain and produce the albino rainbow trout that offspring is all blood-shot eye illness ball look yellow, namely obtain the rainbow trout albino mutation strain with quick growth characteristics.
2. the genetic improvement method of a kind of rainbow trout albino mutation strain growth rate according to claim 1, it is characterized in that in step 2, female parent and male parent being hybridized, obtain F1 generation seed be short-day, water temperature be 10 ~ 11 DEG C, under rainbow trout enters the condition of breeding period, female parent and male parent are carried out hybridization combo separately according to 1:1 ratio, obtains F1 generation seed.
3. the genetic improvement method of a kind of rainbow trout albino mutation strain growth rate according to claim 1, is characterized in that the F2 generation individual independent seed selection cylinder at diameter 0.8m × high 0.6m × dark 0.3m described in step 4 is raised to 50g.
4. the genetic improvement method of a kind of rainbow trout albino mutation strain growth rate according to claim 1, it is characterized in that selecting in step 5 the albino rainbow trout healthy individuals with blood-shot eye illness ball look yellow to refer to: grow to Individual Quality and reach 50g, the individuality with the albino rainbow trout of blood-shot eye illness ball look yellow of long more than the 16cm of body.
5. the genetic improvement method of a kind of rainbow trout albino mutation strain growth rate according to claim 1, it is characterized in that carrying out in step 5 anaesthetizing is adopt concentration to be that the Phenoxyethanol solution of 0.5mL/L carries out.
6. the genetic improvement method of a kind of rainbow trout albino mutation strain growth rate according to claim 1, the water temperature that it is characterized in that carrying out in step 5 electronic marker implantation is more than 12 DEG C.
7. the genetic improvement method of a kind of rainbow trout albino mutation strain growth rate according to claim 1, it is characterized in that carrying out in step 5 before electronic marker with mark after to injection site disinfection, concrete conditions for sterilization is: disinfecting drug selects volumn concentration to be the ethanolic solution of 75% ~ 80%, chip, injection needle volumn concentration is 75% alcohol immersion sterilization.
8. the genetic improvement method of a kind of rainbow trout albino mutation strain growth rate according to claim 1, the injection site that it is characterized in that carrying out in step 5 electronic marker is by abdomeinal fin leading edge 1.5cm, syringe needle and fish body belly are 45° angle, oblique cutting enters intrathecal, is then the parallel chip being pushed forward into electronic marker with fish length axle.
CN201410557859.2A 2014-10-20 2014-10-20 A kind of genetic improvement method of the rainbow trout albino mutation strain speed of growth Expired - Fee Related CN104304115B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107372219A (en) * 2017-08-16 2017-11-24 中国水产科学研究院黑龙江水产研究所 A kind of selection and use method for improving rainbow trout fillet fat content

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JP2011244762A (en) * 2010-05-28 2011-12-08 Iwate Prefecture Method and apparatus for fish egg controlling salmonidae fish and fish egg control for salmonidae fish
CN103651213A (en) * 2013-12-12 2014-03-26 苏州市相城区新时代特种水产养殖场 Artificial fish breeding method
CN203748447U (en) * 2014-02-17 2014-08-06 中国水产科学研究院黑龙江水产研究所 Device applicable to fixing fertilized eggs of rainbow trout

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Publication number Priority date Publication date Assignee Title
CN101606500A (en) * 2009-07-22 2009-12-23 北京顺通虹鳟鱼养殖中心 Technical method of artificial propagation of rainbow trout
CN102047850A (en) * 2009-11-05 2011-05-11 中国水产科学研究院黑龙江水产研究所 Breeding and hatching device for salmon and trout
JP2011244762A (en) * 2010-05-28 2011-12-08 Iwate Prefecture Method and apparatus for fish egg controlling salmonidae fish and fish egg control for salmonidae fish
CN103651213A (en) * 2013-12-12 2014-03-26 苏州市相城区新时代特种水产养殖场 Artificial fish breeding method
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107372219A (en) * 2017-08-16 2017-11-24 中国水产科学研究院黑龙江水产研究所 A kind of selection and use method for improving rainbow trout fillet fat content

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