CN104266860B - A kind of spring brain piece sampler and its sampling method - Google Patents
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- 210000004556 brain Anatomy 0.000 title claims abstract description 63
- 238000005070 sampling Methods 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 11
- 239000011521 glass Substances 0.000 claims abstract description 32
- 238000012546 transfer Methods 0.000 claims abstract description 6
- 210000003813 thumb Anatomy 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 7
- 239000004033 plastic Substances 0.000 claims description 6
- 239000012531 culture fluid Substances 0.000 claims description 3
- 210000004247 hand Anatomy 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 11
- 238000011160 research Methods 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 3
- 238000005457 optimization Methods 0.000 description 4
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- 238000010586 diagram Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
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Abstract
本发明公开了一种弹簧式脑片取样器及其取样方法，该取样器包括玻璃吸头、弯形管和可控活塞部件，弯形管呈圆形一端通过连接头与可控活塞部件连接，弯形管的另一端与玻璃吸头相连；可控活塞部件包括顶部、连接杆以及活塞体部，活塞体部的上端通过连接杆与活塞顶部活动连接，活塞体部内设有弹簧，弹簧一端通过活动面板与活塞连接杆活动连接，弹簧的另一端固定在活塞体部下端的固定面板上。其取样方法是将吸头靠近脑片轻按活塞顶部，然后慢慢松开吸取脑片即可。本发明为相关专业科研实验提供了结构紧凑、操作简单且稳定可靠的脑片取样器，为神经电生理实验研究人员提高了脑片转移的效率和最大限度地减少了对脑片的损伤，提高了脑片组织的存活率。
The invention discloses a spring-type brain slice sampler and its sampling method. The sampler includes a glass suction head, a curved tube and a controllable piston part. The round end of the curved tube is connected to the controllable piston part through a connecting head. , the other end of the curved tube is connected with the glass suction head; the controllable piston part includes a top, a connecting rod and a piston body, the upper end of the piston body is movably connected with the top of the piston through a connecting rod, and a spring is arranged inside the piston body. One end is movably connected with the piston connecting rod through the movable panel, and the other end of the spring is fixed on the fixed panel at the lower end of the piston body. The sampling method is to put the suction head close to the brain slice and lightly press the top of the piston, and then slowly release it to suck the brain slice. The invention provides a brain slice sampler with compact structure, simple operation, stable and reliable for relevant professional scientific research experiments, improves the efficiency of brain slice transfer and minimizes the damage to brain slices for neuroelectrophysiological experiment researchers, and improves survival rate of brain slices.
Description
技术领域technical field
本发明涉及一种脑片电生理实验装置，具体涉及一种弹簧式脑片取样器及其取样方法。The invention relates to a brain slice electrophysiological experiment device, in particular to a spring type brain slice sampler and a sampling method thereof.
背景技术Background technique
目前在高等院校及科研机构，许多相关专业的电生理实验研究都要进行记忆、睡眠、思维活动、脑功能等实验，尤其是医学药学等相关专业，需要进行大量的与神经系统活动相关的实验研究。现在普遍采用的脑片取样实验装置（如说明书附图2所示），普遍采用普通的滴管，由于管径偏小，极易造成脑片扭曲变形，也有人直接用小勺，操作时都会很容易损伤脑片导致实验失败。At present, in colleges and universities and scientific research institutions, electrophysiological experiments in many related majors require experiments on memory, sleep, thinking activities, and brain functions. Experimental Study. The commonly used experimental device for sampling brain slices (as shown in Figure 2 of the manual) generally uses an ordinary dropper. Due to the small diameter of the tube, it is easy to cause the brain slices to be twisted and deformed. Some people directly use a small spoon. It is easy to damage the brain slices and cause the experiment to fail.
发明内容Contents of the invention
发明目的：本发明的目的是为了解决现有技术中的不足，提供一种结构合理、性能良好且操作稳定的脑片取样装置及其取样方法。Purpose of the invention: The purpose of the present invention is to solve the deficiencies in the prior art and provide a brain slice sampling device and its sampling method with reasonable structure, good performance and stable operation.
技术方案：本发明所述的一种弹簧式脑片取样器，包括玻璃吸头、弯形管和可控活塞，所述玻璃吸头为前方后圆形；所述弯形管的一端通过连接头与所述可控活塞部件连接，所述弯形管的另一端与玻璃吸头相连接，所述可控活塞部件包括顶部、连接杆以及活塞体部，所述活塞体部的上端通过连接杆与所述顶部活动连接，所述活塞体部内还设有弹簧，所述弹簧一端通过活动面板与所述连接杆活动连接，所述弹簧的另一端固定在活塞体部下端的固定面板上。Technical solution: A spring-type brain slice sampler according to the present invention includes a glass suction head, a curved tube and a controllable piston. The glass suction head is circular in front and rear; one end of the curved tube is connected to The head is connected to the controllable piston part, and the other end of the curved tube is connected to the glass suction head. The controllable piston part includes a top, a connecting rod and a piston body, and the upper end of the piston body is connected to The rod is movably connected with the top, and a spring is also arranged in the piston body, one end of the spring is movably connected with the connecting rod through a movable panel, and the other end of the spring is fixed on the fixed panel at the lower end of the piston body.
作为优化，所述活动面板的材质为优质弹性橡胶。As an optimization, the material of the movable panel is high-quality elastic rubber.
作为优化，所述活塞体部的材质为硬质塑料。As an optimization, the material of the piston body is hard plastic.
作为优化，所述弯形管为硬质微弹塑料弯形管，弯形管的弧度为110-120度，管内径为3毫米。As an optimization, the curved tube is a hard micro-elastic plastic curved tube, the arc of the curved tube is 110-120 degrees, and the inner diameter of the tube is 3 mm.
作为优化，所述玻璃吸头为前方后圆的玻璃吸头，且包含两套玻璃吸头，大的边长为6毫米、小的边长为4毫米。As an optimization, the glass suction head is a glass suction head with a front and a rear circle, and includes two sets of glass suction heads, the largest side length is 6 mm, and the small side length is 4 mm.
本发明还公开了一种弹簧式脑片取样器的取样方法，包括如下步骤：The invention also discloses a sampling method of a spring-type brain slice sampler, comprising the following steps:
（1）取出洗净干燥好玻璃吸头和弯形管，将弯形管一端与玻璃吸头的圆形端连接好；(1) Take out the cleaned and dried glass tip and the curved tube, and connect one end of the curved tube to the round end of the glass tip;
（2）将带有玻璃吸头的弯形管另一端与可调节活塞连接头相连接且调整到最佳方向；(2) Connect the other end of the curved tube with the glass suction tip to the adjustable piston connector and adjust to the best direction;
（3）用手固定活塞体部，大拇指紧贴活塞的顶部；(3) Fix the body of the piston with your hands, and keep your thumb close to the top of the piston;
（4）将该脑片取样器移至盛有刚切好的脑片容器上方，轻压下大拇指，将方形吸头伸入到想取的脑片附近，慢慢抬起大拇指，使得目标脑片吸至弯形管中；(4) Move the brain slice sampler to the top of the container containing the freshly cut brain slices, gently press down the thumb, extend the square suction head near the desired brain slice, and slowly lift the thumb so that The target brain slice is sucked into the curved tube;
（5）再将该脑片取样器转移到事先准备好的盛有培养液脑片孵育器的液面下，再轻轻按压大拇指，将所取脑片放入脑片孵育器中，完成脑片的取样。(5) Then transfer the brain slice sampler to the liquid surface of the brain slice incubator filled with culture fluid prepared in advance, and then gently press the thumb to put the taken brain slice into the brain slice incubator, and complete Sampling of brain slices.
有益效果：本发明具有如下有益效果：Beneficial effect: the present invention has following beneficial effect:
（1）采用玻璃前方后圆形吸头，避免在取样时损伤脑片而且便于连接；(1) Adopt glass front and rear circular suction heads to avoid damage to brain slices during sampling and facilitate connection;
（2）弯形管便于调节吸头的角度，使操作更为方便精确；(2) The curved tube is easy to adjust the angle of the suction head, making the operation more convenient and precise;
（3）微弹簧操纵杆使取样更为灵活稳定，可以大大提高脑片的安全性；(3) The micro-spring joystick makes sampling more flexible and stable, which can greatly improve the safety of brain slices;
（4）脑片取样操作过程稳定、准确，工作性能良好。(4) The brain slice sampling operation process is stable and accurate, and the working performance is good.
可见本发明为相关专业科研实验提供了结构紧凑、操作简单且稳定可靠的脑片取样器，为神经电生理实验研究人员提高了脑片转移的效率和最大限度地减少了对脑片的损伤，提高了脑片组织的存活率。It can be seen that the present invention provides a compact, simple, stable and reliable brain slice sampler for relevant professional scientific research experiments, improves the efficiency of brain slice transfer and minimizes the damage to brain slices for neuroelectrophysiological experiment researchers, Improves the survival rate of brain slice tissue.
附图说明Description of drawings
图1为本发明的一个实施例的结构示意图。Fig. 1 is a schematic structural diagram of an embodiment of the present invention.
图2为现有技术中的取样装置结构图。Fig. 2 is a structural diagram of a sampling device in the prior art.
具体实施方式detailed description
如图1所示的一种弹簧式脑片取样器，包括玻璃吸头9、弯形管8和可控活塞部件，弯形管8的一端通过连接头7与所述可控活塞部件连接，所述弯形管8的另一端与前方后圆的玻璃吸头9相连；所述可控活塞部件包括顶部1、连接杆2以及活塞体部3，所述活塞体部3的上端通过连接杆2与所述顶部1活动连接，所述活塞体部3内还设有弹簧5，所述弹簧5一端通过活动面板4与所述连接杆2活动连接，所述弹簧5的另一端固定在活塞体部3底部的固定面板6上，其中活动面板4的材质为优质弹性橡胶，活塞体部3的材质为硬质塑料，弯形管8为硬质微弹塑料弯形管，弯形管8的弧度为110-120度，管内径为3毫米。玻璃吸头9为前方后圆的玻璃吸头，且包含两套玻璃吸头，大的边长为6毫米、小的边长为4毫米。A kind of spring type brain slice sampler as shown in Figure 1, comprises glass suction tip 9, curved tube 8 and controllable piston part, one end of curved tube 8 is connected with described controllable piston part by connector 7, The other end of the curved tube 8 is connected to the front and rear round glass suction head 9; the controllable piston part includes a top 1, a connecting rod 2 and a piston body 3, and the upper end of the piston body 3 passes through the connecting rod 2 is movably connected with the top 1, and a spring 5 is also provided in the piston body 3, one end of the spring 5 is movably connected with the connecting rod 2 through the movable panel 4, and the other end of the spring 5 is fixed on the piston On the fixed panel 6 at the bottom of the body 3, the material of the movable panel 4 is high-quality elastic rubber, the material of the piston body 3 is hard plastic, the curved tube 8 is a hard micro-elastic plastic curved tube, and the curved tube 8 The arc is 110-120 degrees, and the inner diameter of the tube is 3mm. The glass suction head 9 is a glass suction head with a front and rear circle, and includes two sets of glass suction heads, the large side length is 6 mm, and the small side length is 4 mm.
本发明的弹簧式脑片取样器具体的取样方法包括如下步骤：The concrete sampling method of spring-type brain slice sampler of the present invention comprises the following steps:
（1）取出洗净干燥好玻璃吸头和弯形管，将弯形管一端与玻璃吸头的圆形端连接好；(1) Take out the cleaned and dried glass tip and the curved tube, and connect one end of the curved tube to the round end of the glass tip;
（2）将带有玻璃吸头的弯形管另一端与可调节活塞连接头相连接且调整到最佳方向；(2) Connect the other end of the curved tube with the glass suction tip to the adjustable piston connector and adjust to the best direction;
（2）用手固定活塞体部，大拇指紧贴活塞的顶部；(2) Fix the body of the piston with your hands, and keep your thumb close to the top of the piston;
（3）将该脑片取样器移至盛有刚切好的脑片容器上方，轻压下大拇指，将方形吸头伸入到想取的脑片附近，慢慢抬起大拇指，使得目标脑片吸至玻璃吸头中；(3) Move the brain slice sampler to the top of the container containing the freshly cut brain slices, gently press down the thumb, insert the square suction head near the desired brain slice, and slowly lift the thumb so that Aspirate the target brain slice into the glass tip;
（4）再将该脑片取样器转移到事先准备好的盛有培养液脑片孵育器的液面下，再轻轻按压大拇指，将所取脑片放入脑片孵育器中，完成脑片的取样。(4) Then transfer the brain slice sampler to the liquid surface of the brain slice incubator filled with the culture fluid prepared in advance, and then gently press the thumb to put the brain slice into the brain slice incubator, and complete Sampling of brain slices.
本装置中的活塞部是本实验操作的重要环节，可以加大实验操作的稳定性。脑片取样器的头部采用方形头部，在很大程度上降低了对脑片的损伤；弯形管可以调节取脑片的最佳方向，可控的操作杆的应用，完全避免了因移动脑片时的摇晃和震动等不可预测的错误操作。本装置结构稳定可靠，具体实验操作简单易行，清洗方便，适合各高校、科研机构相关学科专业领域的实验室广泛使用，并可显著提高实验的成功率。The piston part in the device is an important part of the experimental operation, which can increase the stability of the experimental operation. The head of the brain slice sampler adopts a square head, which greatly reduces the damage to the brain slices; the curved tube can adjust the best direction for taking brain slices; Unpredictable erroneous operations such as shaking and vibration when moving the brain slice. The structure of the device is stable and reliable, the specific experimental operation is simple and easy, and it is convenient to clean. It is suitable for wide use in laboratories in related disciplines and professional fields of universities and scientific research institutions, and can significantly improve the success rate of experiments.
本实验装置发明结构合理，主要体现在以下几方面：⑴采用玻璃方型吸头，避免在取样时损伤脑片；⑵弯形管便于控制吸头方向；⑶微弹簧操纵杆使取样更为灵活精确、安全稳定；⑷脑片取样工作性能良好。The invention of this experimental device has a reasonable structure, which is mainly reflected in the following aspects: (1) the glass square suction head is used to avoid damage to the brain slices during sampling; (2) the curved tube is convenient for controlling the direction of the suction head; (3) the micro-spring joystick makes sampling more flexible Accurate, safe and stable; (4) Brain slice sampling works well.
本发明为相关专业科研实验提供了结构紧凑、操作简单且稳定可靠的脑片取样器，为神经电生理实验研究人员提高了脑片转移的效率和最大限度地减少了对脑片的损伤，提高了脑片组织的存活率。The invention provides a brain slice sampler with compact structure, simple operation, stable and reliable for relevant professional scientific research experiments, improves the efficiency of brain slice transfer and minimizes the damage to brain slices for neuroelectrophysiological experiment researchers, and improves survival rate of brain slices.
本发明的技术内容及技术特征已揭示如上，然而熟悉本领域的技术人员仍可能基于本发明的揭示而作种种不背离本发明精神的替换及修饰，因此，本发明保护范围应不限于实施例所揭示的内容，而应包括各种不背离本发明的替换及修饰，并为本专利申请权利要求所涵盖。The technical contents and technical features of the present invention have been disclosed above, but those skilled in the art may still make various replacements and modifications based on the disclosure of the present invention without departing from the spirit of the present invention. Therefore, the protection scope of the present invention should not be limited to the embodiments The disclosed content should include various replacements and modifications that do not depart from the present invention, and are covered by the claims of this patent application.
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| US4418692A (en) * | 1978-11-17 | 1983-12-06 | Guay Jean Louis | Device for treating living tissue with an electric current |
| CN2076406U (en) * | 1990-08-29 | 1991-05-08 | 许肖宁 | Dilution-proof bone marrow sampler |
| US5483951A (en) * | 1994-02-25 | 1996-01-16 | Vision-Sciences, Inc. | Working channels for a disposable sheath for an endoscope |
| CN2478547Y (en) * | 2001-06-14 | 2002-02-27 | 张平兴 | Human body partial epiderm rubbing and dirt removing instrument |
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| US20050256425A1 (en) * | 2004-05-11 | 2005-11-17 | Inpro Biotechnology, Inc. | Device, system and method for extracting and preparing brain tissue |
| CN2857833Y (en) * | 2005-12-02 | 2007-01-17 | 顾永成 | Deep tissue aspiration pathological biopsy needle |
| RU2333012C1 (en) * | 2007-03-12 | 2008-09-10 | Виктор Петрович Ярошенко | Device for aspiration of liquid contents of cystic formations |
| CN201469315U (en) * | 2009-08-27 | 2010-05-19 | 陈常霞 | Multifunctional endometrium sampler |
| CN201469316U (en) * | 2009-09-11 | 2010-05-19 | 李代瑛 | Living tissue puncture device for pathological section |
| CN201524096U (en) * | 2009-10-15 | 2010-07-14 | 于秀荣 | Closed sampler for sampling secretion or shedding tissues in gynecologic cervix |
| CN201564511U (en) * | 2009-12-29 | 2010-09-01 | 蒋庆玲 | Intrauterine endometrium sampling device |
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| US9968340B2 (en) * | 2012-10-24 | 2018-05-15 | William Zinnanti | Biopsy device with automatic aspiration |
| CN203564305U (en) * | 2013-10-25 | 2014-04-30 | 单士普 | Tumor extractor |
| CN203688274U (en) * | 2013-11-15 | 2014-07-02 | 中国科学院生物物理研究所 | Tissue chip sampling gun |
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