CN104232467A - Molecular hybridization reaction chamber - Google Patents

Molecular hybridization reaction chamber Download PDF

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Publication number
CN104232467A
CN104232467A CN 201410414117 CN201410414117A CN104232467A CN 104232467 A CN104232467 A CN 104232467A CN 201410414117 CN201410414117 CN 201410414117 CN 201410414117 A CN201410414117 A CN 201410414117A CN 104232467 A CN104232467 A CN 104232467A
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CN
China
Prior art keywords
reaction chamber
hybridization
watercourse
reaction
liquid
Prior art date
Application number
CN 201410414117
Other languages
Chinese (zh)
Inventor
傅坚
陈伟勇
蔡树衡
谢杰
Original Assignee
广东凯普生物科技股份有限公司
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Application filed by 广东凯普生物科技股份有限公司 filed Critical 广东凯普生物科技股份有限公司
Priority to CN 201410414117 priority Critical patent/CN104232467A/en
Publication of CN104232467A publication Critical patent/CN104232467A/en

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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0803Disc shape
    • B01L2300/0806Standardised forms, e.g. compact disc [CD] format
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices

Abstract

The invention provides a molecular hybridization reaction chamber which is characterized in that the reaction chamber is in a shape of a square box, the upper shell of the reaction chamber is provided with a plurality of inwardly dished square reaction cases; the center of each reaction case is provided with a liquid discharge hole; the lower parts of the liquid discharge holes are mutually connected through a transverse watercourse, and a drainage nozzle arranged on the lower shell of the reaction chamber is used for discharging the liquid; the watercourse is transversely led to the side wall of the reaction chamber to form a watercourse port; and the watercourse port is threaded and is tightened and sealed with a nut. The molecular hybridization reaction chamber can implement automation of the full-automatic hybridization supporting film platform, can form negative pressure inside through pumping, and can adsorb and discharge the liquid on the film surface through negative pressure, thereby obtaining the sufficient hybridization process.

Description

分子杂交反应室 Hybridization reaction chamber

技术领域 FIELD

[0001] 本发明涉及生物领域,具体是一种分子杂交反应室。 [0001] The present invention relates to the field of biological, in particular a hybridization reaction chamber.

背景技术 Background technique

[0002] 生物芯片技术就是通过微加工工艺同时将大量的探针分子固定到固相支持物上后与标记的样品分子进行杂交,通过检测每个探针分子的杂交信号强度进而获取样品分子的数量和序列信息并进行高效的解读和分析。 [0002] Biochip is through micromachining processes at the same time a large number of probe molecules are immobilized to a solid support for hybridization with a sample molecule was labeled on the rear, and thus obtaining a sample molecules detected by hybridization signal intensity for each probe molecule the number and sequence information and efficient interpretation and analysis.

[0003] 生物芯片技术主要应用于克隆基因的筛选、酶切图谱的制作、基因突变及多态性分析、疾病诊断和预防、药物筛选、基因测序等方面。 [0003] biochip technology is mainly used in the production screening, enzyme map of the cloned gene, mutation and polymorphism analysis, diagnosis and prevention of diseases, drug screening, and other aspects of gene sequencing. 此外,生物芯片的不断发展,在临床诊断上的应用逐步扩大,在农业、司法鉴定、环境保护等行业也得到广泛的应用。 In addition, the development of bio-chips, used in clinical diagnosis and gradually expand, agriculture, forensic, environmental protection and other industries have also been widely used. 生物芯片的应用,具有制备相对简单、操作方便、成本低廉等优势,有利于市场的推广,具有巨大发展潜力和良好的发展前景。 Biochip, with the preparation of a relatively simple, easy operation, low cost and other advantages, is conducive to the promotion of the market, has great potential and good prospects for development.

[0004]目前分子杂交大多采用传统杂交炉,无法实现完全自动化杂交,操作比较复杂,尤其在杂交过程中无法实现DNA或RNA的快速结合,清洗困难,杂交时间过长。 [0004] Currently most conventional hybridization oven hybridization, hybridization can not be fully automated, the operation is more complex, especially in rapid hybridization can not bind DNA or RNA, difficult to clean, long hybridization time.

发明内容 SUMMARY

[0005] 为解决上述问题,本发明提供了一种分子杂交反应室。 [0005] In order to solve the above problems, the present invention provides a hybridization reaction chamber.

[0006] 分子杂交反应室主要是配套全自动核酸分子杂交仪的关键部件,是生物芯片杂交过程的载体,能够实现包括杂交过程的温度控制,杂交液、洗涤液、酶标液、显色液及水的清洗,自动完成液体排放等功能。 [0006] The hybridization reaction chamber mainly automated nucleic acid hybridization instrument supporting the key member, the carrier is a biochip hybridisation process can be achieved during hybridization including temperature control, the hybridization solution, washing solution, enzyme solution, color developing solution and water washing, the liquid discharge functions automatically.

[0007] 本发明的分子杂交反应室,其特征在于,反应室为方形盒状,在反应室的上壳设有多个内凹的方形反应格,每个反应格中心设有排液孔,排液孔下方通过横向的水道相互连接,并通过设在反应室下壳的排水水嘴进行排液;水道横向通至反应室的侧壁成水道口,水道口设为螺纹状,并用螺母旋紧密封。 [0007] The hybridization reaction chamber of the present invention is characterized in that the reaction chamber is a square box shape, the shell of the reaction chamber is provided with a plurality of square concave reaction cells, each reaction cell has a central drainage hole, below the drainage hole are connected by transverse waterways each other, and a liquid discharge through the drain nozzle provided in a reaction chamber under the shell; watercourse transverse to the side wall of the reaction chamber through the crossing into water, the water crossing set threaded, and are screwed with nuts tight seal.

[0008] 本发明的上述结构设置能够实现全自动杂交载膜平台自动化,通过泵吸在反应室内部形成负压,并由负压将膜面液体吸附排出,得到充分杂交的过程。 [0008] The above-described structure is provided according to the present invention enables the carrier film hybrid automatic automation platform, by pumping the reaction chamber forming a negative pressure inside the negative pressure by discharging the adsorbed liquid film surface, the hybridization of the full process.

附图说明 BRIEF DESCRIPTION

[0009] 图1为本发明分子杂交反应室的结构示意图(为便于视图显示,水道口的螺纹和螺母不画出,以下附图同此处理)。 Schematic configuration [0009] FIG. 1 of the present invention hybridization reaction chamber (for ease of display view, the water crossing thread and a nut not shown, this process with the following drawings).

[0010] 图2为本发明分子杂交反应室的立体图。 [0010] Fig 2 a perspective view of the reaction chamber of the hybridization of the present invention.

[0011] 图3为本发明分子杂交反应室另一个角度的立体图。 [0011] FIG. 3 is a perspective view of another angular hybridization reaction chamber of the present invention.

[0012] 图4为图1的左视图。 [0012] FIG. 4 is a left side view of FIG. 1.

[0013] 图5为图1的后视图。 [0013] FIG. 5 is a rear view of FIG. 1.

[0014] 图6为图1的AA剖视图。 [0014] FIG. 6 is a cross-sectional view AA of FIG. 1.

[0015] 图7为图1的BB剖视图。 [0015] FIG. 7 is a cross-sectional view BB of FIG. 1.

[0016] 图8为图5的CC剖视图。 [0016] FIG. 8 is a cross-sectional view of FIG. 5 CC.

[0017] 图9为图1的俯视图。 [0017] FIG. 9 is a top view of FIG. 1.

[0018] 图10为图1的仰视图。 [0018] FIG. 10 is a bottom view of FIG. 1 FIG.

[0019] 图11为图10的DD剖视图。 [0019] FIG. 11 is a cross-sectional view of FIG. 10 DD.

具体实施方式 Detailed ways

[0020] 如图1〜11所示,本发明的分子杂交反应室,其特征在于,反应室为方形盒状,在反应室的上壳I设有多个内凹的方形反应格2,每个反应格2中心设有排液孔3,排液孔3下方通过横向的水道4相互连接,并通过设在反应室下壳5的排水水嘴6进行排液;水道4横向通至反应室的侧壁7成水道口8,水道口8设为螺纹状,并用螺母旋紧密封。 [0020] As shown, the hybridization reaction chamber of the present invention, wherein 1~11, the reaction chamber is a rectangular box-like, on the housing of the reaction chamber I is provided with a plurality of square concave reaction cells 2, each the reaction cell 2 is provided with a central drain hole 3, beneath the discharge ports 3 are connected by a transverse waterways 4 are, provided in the discharge nozzle and through the housing 5 of the 6 reaction liquid discharge chamber; 4 transverse through the reaction chamber to waterways the side wall 7 8 crossing into water, the water crossing to 8 threaded, and a nut screwed with a seal.

Claims (1)

1.分子杂交反应室,其特征在于,反应室为方形盒状,在反应室的上壳设有多个内凹的方形反应格,每个反应格中心设有排液孔,排液孔下方通过横向的水道相互连接,并通过设在反应室下壳的排水水嘴进行排液;水道横向通至反应室的侧壁成水道口,水道口设为螺纹状,并用螺母旋紧密封。 1. The hybridization reaction chamber, characterized in that the reaction chamber is a rectangular box-shaped, provided with a plurality of square concave shell of the reaction chamber in the reaction cells, each reaction cell has a central drainage hole, below the discharge ports connected to each other by a transverse waterway, and through the drain nozzle provided in a reaction chamber under the shell for discharging liquid; watercourse transverse to the side wall of the reaction chamber through the crossing into water, the water crossing set threaded, and a nut screwed with a seal.
CN 201410414117 2014-08-21 2014-08-21 Molecular hybridization reaction chamber CN104232467A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1197413A (en) * 1995-09-22 1998-10-28 伯莱克斯实验室公司 Appts. and process for multiple chemical reactions
CN1882382A (en) * 2003-11-24 2006-12-20 生物概念股份有限公司 Microarray hybridization device
CN201545839U (en) * 2009-07-06 2010-08-11 中山大学达安基因股份有限公司;佛山达安医疗设备有限公司 Full-automatic molecule hybridization instrument
CN102083533A (en) * 2008-04-10 2011-06-01 芬兰技术研究中心 Microfluidic chip devices and their use
CN102445557A (en) * 2011-09-27 2012-05-09 深圳市亚辉龙生物科技有限公司 Full-automatic immunity analyzer and detection method thereof
CN202671526U (en) * 2011-12-28 2013-01-16 广州好芝生物科技有限公司 Nucleic acid molecule hybridization instrument

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1197413A (en) * 1995-09-22 1998-10-28 伯莱克斯实验室公司 Appts. and process for multiple chemical reactions
CN1882382A (en) * 2003-11-24 2006-12-20 生物概念股份有限公司 Microarray hybridization device
CN102083533A (en) * 2008-04-10 2011-06-01 芬兰技术研究中心 Microfluidic chip devices and their use
CN201545839U (en) * 2009-07-06 2010-08-11 中山大学达安基因股份有限公司;佛山达安医疗设备有限公司 Full-automatic molecule hybridization instrument
CN102445557A (en) * 2011-09-27 2012-05-09 深圳市亚辉龙生物科技有限公司 Full-automatic immunity analyzer and detection method thereof
CN202671526U (en) * 2011-12-28 2013-01-16 广州好芝生物科技有限公司 Nucleic acid molecule hybridization instrument

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