CN104206176A - Wild culture method of ganoderma lucidum - Google Patents
Wild culture method of ganoderma lucidum Download PDFInfo
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- CN104206176A CN104206176A CN201410484853.7A CN201410484853A CN104206176A CN 104206176 A CN104206176 A CN 104206176A CN 201410484853 A CN201410484853 A CN 201410484853A CN 104206176 A CN104206176 A CN 104206176A
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- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 18
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 17
- 238000011081 inoculation Methods 0.000 claims abstract description 14
- 230000003716 rejuvenation Effects 0.000 claims abstract description 5
- 238000003306 harvesting Methods 0.000 claims abstract description 4
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 241000222336 Ganoderma Species 0.000 claims description 59
- 241000207961 Sesamum Species 0.000 claims description 39
- 235000003434 Sesamum indicum Nutrition 0.000 claims description 39
- 239000004035 construction material Substances 0.000 claims description 26
- 230000001580 bacterial Effects 0.000 claims description 25
- 230000001954 sterilising Effects 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 239000002023 wood Substances 0.000 claims description 6
- 240000000516 Acer negundo Species 0.000 claims description 3
- 235000004422 Acer negundo Nutrition 0.000 claims description 3
- 244000144730 Amygdalus persica Species 0.000 claims description 3
- 210000000481 Breast Anatomy 0.000 claims description 3
- 241001070941 Castanea Species 0.000 claims description 3
- 235000014036 Castanea Nutrition 0.000 claims description 3
- 235000006040 Prunus persica var persica Nutrition 0.000 claims description 3
- 241000124033 Salix Species 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 230000012010 growth Effects 0.000 description 6
- 240000000218 Cannabis sativa Species 0.000 description 3
- 230000000050 nutritive Effects 0.000 description 3
- 230000003698 anagen phase Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 206010003549 Asthenia Diseases 0.000 description 1
- 208000004981 Coronary Disease Diseases 0.000 description 1
- 206010012601 Diabetes mellitus Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 208000006454 Hepatitis Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 208000007443 Neurasthenia Diseases 0.000 description 1
- 241000222341 Polyporaceae Species 0.000 description 1
- 229960000716 TONICS Drugs 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000001488 breeding Effects 0.000 description 1
- 230000023298 conjugation with cellular fusion Effects 0.000 description 1
- 201000008739 coronary artery disease Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006011 modification reaction Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001850 reproductive Effects 0.000 description 1
- 201000003068 rheumatic fever Diseases 0.000 description 1
- 230000001340 slower Effects 0.000 description 1
- 210000004215 spores Anatomy 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002195 synergetic Effects 0.000 description 1
- 230000001225 therapeutic Effects 0.000 description 1
- 230000001256 tonic Effects 0.000 description 1
- 235000015961 tonic Nutrition 0.000 description 1
- 230000001131 transforming Effects 0.000 description 1
- 230000021037 unidirectional conjugation Effects 0.000 description 1
Abstract
The invention discloses a wild culture method of ganoderma lucidum. The method comprises the following steps of 1, wild ganoderma lucidum strain selection; 2, culture bagged material preparation; 3, inoculation and mycelium culture; 4, cultivation field selection; 5, wild cultivation; and 6, harvesting and strain rejuvenation. The wild culture method of the ganoderma lucidum disclosed by the invention has the advantages that the generated ganoderma lucidum has the same nutritional value as wild ganoderma lucidum, and the yield is obviously higher than that of the wild ganoderma lucidum.
Description
Technical field
The invention belongs to edible mushroom culture technique field, more specifically relate to the wild cultivating method of a kind of glossy ganoderma.
Background technology
Glossy ganoderma is also known as polyporus lucidus, refreshing sesame, sesame grass, celestial grass, auspicious grass, it is the complete stool of the red sesame of Polyporaceae plant or purple sesame, for strengthening by means of tonics, the precious Chinese herbal medicine consolidating righting, modern medicine proves: glossy ganoderma contains several physiological active substances, can regulate, strengthen body immunity, have good synergistic therapeutic action to neurasthenia, rheumatic arthritis, coronary heart disease, hypertension, hepatitis, diabetes, tumour etc.The cultivation of current glossy ganoderma mainly adopts the method for cultivating in booth, although output is higher, because it grows in artificial environment, and cultivation cycle is shorter, and nutritive value is just relatively low; And although Wild ganoderma nutritive value is very high, completely wild glossy ganoderma quantity very little, often somethings can only be found by accident, and not through seeking, and can not meet the consumption demand of people.
Summary of the invention
In order to solve in prior art, the ganoderma lucidum of cultivating in booth is worth lower, and the problem that Wild ganoderma output is too low, the invention provides the wild cultivating method of a kind of glossy ganoderma.
The present invention is achieved through the following technical solutions:
A wild cultivating method for glossy ganoderma, comprises the following steps:
The first step, Wild ganoderma bacterial classification is selected
Select Wild ganoderma as bacterial classification;
Second step, cultivates the preparation of construction materials contract
Select the trunk of broad-leaved tree, be processed into the short section of wood of 10 ~ 50 cm, pack rear sterilization treatment;
3rd step, inoculation and cultural hypha
Cultivation construction materials contract after having carried out sterilization treatment is moved into aseptic inoculation room, bacterial classification and cultivation construction materials contract is put together, under being placed in dark surrounds, cultivates 20 ~ 25 d, until mycelia covers cultivate more than 50% of construction materials contract area;
4th step, the selection of cultivation area
Select north latitude 24 ~ 30 °, the mountain area of the subtropics monsoon climate of east longitude 113 ~ 118 ° is as cultivation area;
5th step, wild cultivating
Broad-leaved tree is chosen from the cultivation area of the 4th step, sesame bed is dug out near the tree root covered by earth of broad-leaved tree, cultivation construction materials contract through the 3rd step cultural hypha is cut and supinely puts into sesame bed, earth is cultivated, after growing young sesame, select young sesame, a young sesame is stayed, self-sow under wild environment in each ripe wooden cross section;
6th step, gathers
After ganoderma lucidum fruitbody is unearthed, after the annual lunar calendar September, loosen the soil and mycelia harvesting is not destroyed to glossy ganoderma.
Select in step at the bacterial classification of the first step, choose north latitude 24 ~ 30 °, on the mountain that east longitude is 113 ~ 118 °, Wild ganoderma is as provenance, selects cap area at 200 ~ 300 cm
2, the Wild ganoderma of cap thickness between 2 ~ 3 cm is as bacterial classification.
Also comprise rejuvenation of spawn step, Wild ganoderma bacterial classification, after 5 ~ 10 times gather, reselects cap area at 200 ~ 300 cm
2, cap thickness carries out inoculating and cultivating as new bacterial classification 2 ~ 3 cm glossy ganodermas.
Prepare at second step to cultivate in the step of construction materials contract, select the broad-leaved tree of trunk diameter between 15 ~ 18 cm, be processed into the short section of wood of 20 ~ 30 cm, after packing, at 100 DEG C, continue boiling sterilization 12 ~ 15 h.
In the step that the 3rd step carries out inoculation and cultural hypha, the environmental temperature of aseptic inoculation room is 25 ~ 30 DEG C, and relative moisture is 65% ~ 75%.
In the wild cultivating step of the 5th step, described sesame bed is 1 ~ 2 cm place above tree root, the bottom of described sesame bed and the distance of tree root upper surface are 1 ~ 2 ㎝, and described sesame bed ensemble is positioned at the earth above broad-leaved tree tree root, and the height of described sesame bed is 15 ~ 18 cm, width is 18 ~ 22 cm, length is the sesame bed of 24 ~ 36 cm, and being 3 ~ 4 cm by the earth thickness of cultivation construction materials contract, is 10 ~ 15 DEG C at temperature, when relative air humidity is 70% ~ 75%, cultivation construction materials contract is imbedded in sesame bed.
In the selection of the cultivation area of described 4th step, select the mountain area of 500 ~ 1200 m height above sea levels as cultivation area, the age of tree of described broad-leaved tree is greater than 5 years, and the sunshade density of described mountain area trees is 50% ~ 80%.
Described broad-leaved tree be chestnut tree, maple tree, peach, willow one or more.
The beneficial effect of the wild cultivating method of a kind of glossy ganoderma of the present invention is mainly reflected in:
(1) the invention provides the wild cultivating method of a kind of glossy ganoderma, cultivate rear just directly moving in wild environment due to Ganoderma lucidum mycelium to grow, cultivate according to the growth cycle of Wild ganoderma completely, absorb the anima between the world and go out, there is the nutritive value be equal to Wild ganoderma.
(2) the invention provides the wild cultivating method of a kind of glossy ganoderma, inoculate after the improved seeds of Wild ganoderma are tamed and cultivate, improve the reproductive efficiency of glossy ganoderma, being significantly higher than the output of Wild ganoderma.
(3) the invention provides the wild cultivating method of a kind of glossy ganoderma, Wild ganoderma bacterial classification, after the inoculation cultivation of 5 ~ 10 times, carries out rejuvenation to bacterial classification, prevents spawn degeneration, ensure that the fine quality of bacterial classification.
Embodiment
Below by specific embodiment, the present invention is described further:
In the present invention, the implication of related notion is:
Sesame bed: the heatable adobe sleeping platform of construction materials contract is cultivated in the placement of excavating in cultivation of glossy ganoderma place.
A wild cultivating method for glossy ganoderma, comprises the following steps:
The first step, Wild ganoderma bacterial classification is selected
Select Wild ganoderma as bacterial classification;
Second step, cultivates the preparation of construction materials contract
Select the trunk of broad-leaved tree, be processed into the short section of wood of 10 ~ 50 cm, pack rear sterilization treatment;
3rd step, inoculation and cultural hypha
Cultivation construction materials contract after having carried out sterilization treatment is moved into aseptic inoculation room, bacterial classification and cultivation construction materials contract is put together, under being placed in dark surrounds, cultivates 20 ~ 25 d, until mycelia covers cultivate more than 50% of construction materials contract area;
4th step, the selection of cultivation area
Select north latitude 24 ~ 30 °, the mountain area of the subtropics monsoon climate of east longitude 113 ~ 118 ° is as cultivation area;
5th step, wild cultivating
Broad-leaved tree is chosen from the cultivation area of the 4th step, sesame bed is dug out near the tree root covered by earth of broad-leaved tree, cultivation construction materials contract through the 3rd step cultural hypha is cut and supinely puts into sesame bed, earth is cultivated, after growing young sesame, select young sesame, a young sesame is stayed, self-sow under wild environment in each ripe wooden cross section;
After such glossy ganoderma maturation, the spore of its different sexes can carry out mating to breed on tree root alive, breeding under this natural conditions is conducive to the preservation of glossy ganoderma fine quality, also the convenient good quality strain to producing is selected, as new bacterial classification parent, decrease the workload that fine quality is found in field.
Be 10 ~ 15 DEG C at temperature, when relative air humidity is 70% ~ 75%, cultivation construction materials contract imbedded in sesame bed, now be generally the lunar calendar two time early spring at the end of month, temperature and humidity is all lower, and all things on earth is recovered, ganoderma lucidum fruitbody poor growth, ganoderma lucidum fruitbody is in the stage adapting to surrounding environment; Along with subsolar point northwards moves, south is carried out warm moist air and is strengthened, and temperature raises gradually, and precipitation frequency also slowly increases, the humidity of air also increases thereupon, to about lunar calendar May, when temperature generally reaches 24 ~ 26 DEG C, and when now the air humidity of the mountain area woods reaches 85% ~ 90%, ganoderma lucidum fruitbody growth rate is accelerated, active ingredient accumulation is more, and the ganoderma lucidum fruitbody quality grown during this period of time is hard, and the nutrient component contained is the highest; Arrived the lunar calendar six, July, along with temperature and humidity increases further, glossy ganoderma enters the alternating growth phase, time fine, temperature is more than 30 DEG C, and air humidity is lower than 80%, glossy ganoderma growth interruption, when weather is overcast and rainy, when temperature reaches 28 ~ 30 DEG C, the air humidity of the mountain area woods reaches 90% ~ 95%, now glossy ganoderma growth rate is very fast, and surface color is more shallow, during this period of time along with weather transformation rain or shine, the wheel line sharpness of border of glossy ganoderma, overcast and rainy turbine line is thinner.The lunar calendar eight, September, air humidity is lower, and day and night temperature increases, and glossy ganoderma enters the uniform growth phase, and growth rate slows down, and the nutrient that early stage absorbs is transformed, and promotes the quality of ganoderma lucidum fruitbody.
6th step, gathers
After ganoderma lucidum fruitbody is unearthed, after the annual lunar calendar September, loosen the soil and mycelia harvesting is not destroyed to glossy ganoderma.
Select in step at the bacterial classification of the first step, choose north latitude 24 ~ 30 °, on the mountain that east longitude is 113 ~ 118 °, Wild ganoderma is as provenance, selects cap area at 200 ~ 300 cm
2, the Wild ganoderma of cap thickness between 2 ~ 3 cm is as bacterial classification.
Also comprise rejuvenation of spawn step, Wild ganoderma bacterial classification, after 5 ~ 10 times gather, reselects cap area at 200 ~ 300 cm
2, cap thickness carries out inoculating and cultivating as new bacterial classification 2 ~ 3 cm glossy ganodermas.
So both ensure that the yield and quality of glossy ganoderma, turn avoid and field all will be gone at every turn to find the work such as new quality variety, acclimatization culture, improve production efficiency.
Prepare at second step to cultivate in the step of construction materials contract, select the broad-leaved tree of trunk diameter between 15 ~ 18 cm, be processed into the short section of wood of 20 ~ 30 cm, after packing, at 100 DEG C, continue boiling sterilization 12 ~ 15 h.
In the step that the 3rd step carries out inoculation and cultural hypha, the environmental temperature of aseptic inoculation room is 25 ~ 30 DEG C, and relative moisture is 65% ~ 75%.
In the wild cultivating step of the 5th step, described sesame bed is 1 ~ 2 cm place above tree root, the bottom of described sesame bed and the distance of tree root upper surface are 1 ~ 2 ㎝, and described sesame bed ensemble is positioned at the earth above broad-leaved tree tree root, and the height of described sesame bed is 15 ~ 18 cm, width is 18 ~ 22 cm, length is the sesame bed of 24 ~ 36 cm, and being 3 ~ 4 cm by the earth thickness of cultivation construction materials contract, is 10 ~ 15 DEG C at temperature, when relative air humidity is 70% ~ 75%, cultivation construction materials contract is imbedded in sesame bed.
In the selection of the cultivation area of described 4th step, select the mountain area of 500 ~ 1200 m height above sea levels as cultivation area, the age of tree of described broad-leaved tree is greater than 5 years, and the sunshade density of described mountain area trees is 50% ~ 80%.
Described broad-leaved tree be chestnut tree, maple tree, peach, willow one or more.
Although inventor has done comparatively detailed elaboration to technical scheme of the present invention and has enumerated; be to be understood that; to those skilled in the art; amendment is made to above-described embodiment or adopts equivalent replacement scheme; this is apparent to those skilled in the art; these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (8)
1. a wild cultivating method for glossy ganoderma, is characterized in that, comprises the following steps:
The first step, Wild ganoderma bacterial classification is selected
Select Wild ganoderma as bacterial classification;
Second step, cultivates the preparation of construction materials contract
Select the trunk of broad-leaved tree, be processed into the short section of wood of 10 ~ 50 cm, pack rear sterilization treatment;
3rd step, inoculation and cultural hypha
Cultivation construction materials contract after having carried out sterilization treatment is moved into aseptic inoculation room, bacterial classification and cultivation construction materials contract is put together, under being placed in dark surrounds, cultivates 20 ~ 25 d, until mycelia covers cultivate more than 50% of construction materials contract area;
4th step, the selection of cultivation area
Select north latitude 24 ~ 30 °, the mountain area of the subtropics monsoon climate of east longitude 113 ~ 118 ° is as cultivation area;
5th step, wild cultivating
Broad-leaved tree is chosen from the cultivation area of the 4th step, sesame bed is dug out near the tree root covered by earth of broad-leaved tree, cultivation construction materials contract through the 3rd step cultural hypha is cut and supinely puts into sesame bed, earth is cultivated, after growing young sesame, select young sesame, a young sesame is stayed, self-sow under wild environment in each ripe wooden cross section;
6th step, gathers
After ganoderma lucidum fruitbody is unearthed, after the annual lunar calendar September, loosen the soil and mycelia harvesting is not destroyed to glossy ganoderma.
2. the wild cultivating method of a kind of glossy ganoderma according to claim 1, it is characterized in that: select in step at the bacterial classification of the first step, choose north latitude 24 ~ 30 °, on the mountain that east longitude is 113 ~ 118 °, Wild ganoderma is as provenance, selects cap area at 200 ~ 300 cm
2, the Wild ganoderma of cap thickness between 2 ~ 3 cm is as bacterial classification.
3. according to the wild cultivating method of a kind of glossy ganoderma according to claim 1 or claim 2, it is characterized in that: also comprise rejuvenation of spawn step, Wild ganoderma bacterial classification, after 5 ~ 10 times gather, reselects cap area at 200 ~ 300 cm
2, cap thickness carries out inoculating and cultivating as new bacterial classification 2 ~ 3 cm glossy ganodermas.
4. the wild cultivating method of a kind of glossy ganoderma according to claim 1, it is characterized in that: prepare to cultivate in the step of construction materials contract at second step, select the broad-leaved tree of trunk diameter between 15 ~ 18 cm, be processed into the short section of wood of 20 ~ 30 cm, after packing, at 100 DEG C, continue boiling sterilization 12 ~ 15 h.
5. the wild cultivating method of a kind of glossy ganoderma according to claim 1, is characterized in that: in the step that the 3rd step carries out inoculation and cultural hypha, the environmental temperature of aseptic inoculation room is 25 ~ 30 DEG C, and relative moisture is 65% ~ 75%.
6. the wild cultivating method of a kind of glossy ganoderma according to claim 1, it is characterized in that: in the wild cultivating step of the 5th step, described sesame bed is 1 ~ 2 cm place above tree root, the bottom of described sesame bed and the distance of tree root upper surface are 1 ~ 2 ㎝, described sesame bed ensemble is positioned at the earth above broad-leaved tree tree root, the height of described sesame bed is 15 ~ 18 cm, width is 18 ~ 22 cm, length is the sesame bed of 24 ~ 36 cm, be 3 ~ 4 cm by cultivating the earth thickness of construction materials contract, it is 10 ~ 15 DEG C at temperature, when relative air humidity is 70% ~ 75%, cultivation construction materials contract is imbedded in sesame bed.
7. the wild cultivating method of a kind of glossy ganoderma according to claim 1, it is characterized in that: in the selection of the cultivation area of described 4th step, select the mountain area of 500 ~ 1200 m height above sea levels as cultivation area, the age of tree of described broad-leaved tree is greater than 5 years, and the sunshade density of described mountain area trees is 50% ~ 80%.
8. the wild cultivating method of a kind of glossy ganoderma according to claim 7, is characterized in that: described broad-leaved tree be chestnut tree, maple tree, peach, willow one or more.
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| CN112106596A (en) * | 2020-09-17 | 2020-12-22 | 永定区德叔家庭农场 | Retention cultivation method of ganoderma lucidum |
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| Publication number | Publication date |
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| CN104206176B (en) | 2017-03-29 |
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