CN104152345B - Micro-fluidic type single cell separator - Google Patents
Micro-fluidic type single cell separator Download PDFInfo
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- CN104152345B CN104152345B CN201410399750.0A CN201410399750A CN104152345B CN 104152345 B CN104152345 B CN 104152345B CN 201410399750 A CN201410399750 A CN 201410399750A CN 104152345 B CN104152345 B CN 104152345B
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- fluid channel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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Abstract
The invention discloses a kind of micro-fluidic type single cell separator, including base, movable disk and fluid channel, base include motor and be connected with motor to innermost being handle slide block structure, movable disk is arranged on the top of base, movable disk be arranged above the inclined-plane to lopsidedness, arranging fluid channel on inclined-plane, the tree fork shape being distributed as branch of fluid channel, the motor in base is by driving movable disk to carry out translational motion innermost being handle slide block structure；It is connected with to innermost being handle slide block structure by fixed plate bottom movable disk；On inclined-plane, fluid channel is provided above transparent cover plate；The material of fluid channel is organic polymer material；In fluid channel, outflow end has the guiding gutter of hollow.The fluid channel of the present invention is on the inclined-plane of movable disk, fluid channel is the microchannel of branching tree, motor in base is by driving movable disk to carry out translational motion innermost being handle slide block structure, the cell suspending liquid added in fluid channel moves in the passage of branching tree, and the cell suspending liquid in fluid channel is isolated unicellular under the motion of movable disk.
Description
Technical field
The present invention relates to a kind of micro-fluidic type single cell separator, belong to biological medicine instrument and equipment.
Background technology
The research of current individual cell level increasingly comes into one's own, and is widely used to single celled DNA cloning, genome
The fields such as order-checking and individual cell level subcellular fraction drug-supplying system research.
Existing unicellular segregation apparatus and method mainly have: 1, fluorescence flow cytometry technology partition method: fluorescence-activation stream
Formula cell separation technology can effectively obtain individual cells, but the performance requirement of flow cytometer is higher, and instrument price
Expensive；2, laser microprobe dating technology: can from tissue isolated mononuclear cell, but be not suitable for cultivation and the amplification of cell,
And need operator the most expertly to grasp cutting technique；3, micro-manipulation instrument: use micro-manipulation technology from single-cell suspension
Liquid is drawn unicellular, but fine operation and valuable instrument make researcher hang back；The defect of above technology is allowed to
Cannot be widely applied.
1998, the Whitesides seminar of Harvard University invented and has utilized that elastic polymer material PDMS's is quick
Replicating the micro-processing method of molding, prepare for micro-fluidic chip, the method is referred to as soft etching technique (soft
lithography).Relative to glass material and silicon materials, this technology processing and fabricating is convenient, micro Process that need not be particularly harsh
Condition and experiment condition, the template once made can repeatedly use, and highly shortened the time needed for chip manufacturing,
Accelerate the speed of research, reduce difficulty and the cost of chip manufacturing.
Polydimethylsiloxane (poly-dimethylsiloxane, PDMS) is the organic polymer based on a kind of silicon
Material, is widely used in the fields such as micro-fluidic, bio-microelectromechanical, can be as micro sprue system, gap filler, lubricant, stealthy eye
Mirrors etc., have low cost, use simple, optical clear, and in the ordinary course of things, are inertia, nontoxic, nonflammable, same to silicon chip
Between have the features such as good adhesiveness.
In view of this, based on this organic polymer material of PDMS, existing unicellular segregation apparatus is improved, with
It is necessary for solving the problems such as cost is high, operation is difficult.
Summary of the invention
It is an object of the invention to provide one can sharp separation unicellular, can operate in Biohazard Safety Equipment, it is ensured that
Sterile working, parts are detachable, use, micro-fluidic type single cell separator simple to operate after sterilizing.
For achieving the above object, the present invention adopts the following technical scheme that
A kind of micro-fluidic type single cell separator, including base, movable disk and fluid channel, base includes motor and and motor
Connect to innermost being handle slide block structure, movable disk is arranged on the top of base, movable disk be arranged above the inclined-plane to lopsidedness,
Arranging fluid channel on inclined-plane, the tree fork shape being distributed as branch of fluid channel, the motor in base is by innermost being handle slide block structure
Movable disk is driven to carry out translational motion.
It is connected with to innermost being handle slide block structure by fixed plate bottom movable disk.
On inclined-plane, fluid channel is provided above transparent cover plate.
The material of fluid channel is organic polymer material.
In fluid channel, outflow end has the guiding gutter of hollow.
Beneficial effect: the fluid channel of the present invention is on the inclined-plane of movable disk, and fluid channel is the microchannel of branching tree, the end
Motor in Zuo by driving movable disk to carry out translational motion innermost being handle slide block structure, the cell suspending liquid added in fluid channel
Moving in the passage of branching tree, the cell suspending liquid in fluid channel will separate in cell suspending liquid under the motion of movable disk
Go out unicellular next.
Accompanying drawing explanation
Fig. 1 is the front view of micro-fluidic type single cell separator；
Fig. 2 is the profile that micro-fluidic type single cell separator splits；
Fig. 3 is the fine structure map of fluid channel；
Fig. 4 is state diagram when isolating unicellular of the micro-fluidic type single cell separator.
In figure: 10 bases, 101 motors, 102 pairs of innermost being handle slide block structures, 20 movable disk, 201 transparency covers
Plate, 202 guiding gutters, 203 fixed plates, 30 fluid channel, 301 add entrance, 302 flow exports, 40 unicellular.
Detailed description of the invention
In order to make the object, technical solutions and advantages of the present invention clearer, below in conjunction with the accompanying drawings with specific embodiment pair
The present invention is described in detail.
As shown in Figures 1 to 4, the micro-fluidic type single cell separator of the present invention is in order to isolate list from cell suspending liquid
Cell 40.
Micro-fluidic type single cell separator, including base 10, movable disk 20 and fluid channel 30, base 10 includes motor 101
And be connected with motor to innermost being handle slide block structure 102, movable disk 20 is arranged on the top of base 10, being arranged above of movable disk 20
To the inclined-plane of lopsidedness, inclined-plane arranges fluid channel 30, the tree fork shape being distributed as branch of fluid channel 30, the horse in base 10
Reach 101 by driving movable disk 20 to carry out translational motion innermost being handle slide block structure 102.
Can be metal or plastic material to innermost being handle slide block structure 102 material, and metal material mainly includes rustless steel
Or aluminum, plastic material is mainly polypropylene.In present embodiment, the material to innermost being handle slide block structure 102 is rustless steel.
Described motor 101 and link innermost being handle slide block structure 102, drives movable disk 20 to carry out translational motion, right
It is attached by fixed plate 203 between innermost being handle slide block structure 102 and movable disk 20.Movable disk 20 material can be metal or can
Plastic material, and metal material mainly includes rustless steel or aluminum, plastic material is mainly polypropylene.In present embodiment, live
The material of Moving plate 20 is polypropylene.
Movable disk 20 appears as ladder structure of right angle from one side, and fluid channel 30 is positioned on the ramp of movable disk 20,
The material of composition fluid channel 30 is organic polymer material, and in present embodiment, the material of composition fluid channel 30 is PDMS.Upper
There is a transparent cover plate 201 on inclined-plane, cover and be contaminated during preventing cell separation in fluid channel 30, at fluid channel 30 end
It is provided with guiding gutter 202, convenient reception isolated unicellular 40.
Fluid channel 30 is fixed by the ramp of binding agent with movable disk 20.Fluid channel 30 is in branching tree, and each section micro-
Runner is S type curve tunnel, and the runner circular arc of fluid channel 30 is divided into different size according to cell size, such as 100 μm, and 150 μm,
200 μm etc., to reduce liquid dropping velocity, convenient separation cell.
Cell suspending liquid enters fluid channel 30 by adding entrance 301, and cell suspending liquid leads at the S type bending of fluid channel 30
In road under the effect of translational motion and gravitional force, carry out shunting and separate, eventually pass through guiding gutter 202 from flow export 302
Ooze (each branches end of fluid channel 30 and guiding gutter one_to_one corresponding), thus isolate unicellular 40.Ensure aseptic, letter
Single, easily operate.Micro-fluidic type single cell separator in present embodiment, after obtaining single intact cell, can be used for unicellular
Photoelectric synchronous detection.
In sum, the micro-fluidic type single cell separator of the present invention is by the motor 101 within base 10 with to innermost being
The linkage of handle slide block structure 102, drives the movable disk 20 of the rectangular trapezoidal shape in side to carry out translational motion, is positioned in movable disk 20
The fluid channel 30 of the inner passage with the bending of S type on inclined-plane is under the effect of translational motion and gravitional force, by cell
Suspension is separated into unicellular 40.The present invention micro-fluidic type single cell separator can operate in Biohazard Safety Equipment, it is ensured that aseptic
Operation, parts are detachable, use after sterilizing, simple to operate, and the material of composition fluid channel is New Type Material polydimethylsiloxane
(PDMS), low cost, there is follow-up cultivation and the detection of good chemical inertness, not damaging cells, beneficially cell.
Claims (5)
1. a micro-fluidic type single cell separator, it is characterised in that: include base, movable disk and fluid channel, described base bag
Include motor and be connected with motor to innermost being handle slide block structure, described movable disk is arranged on the top of base, the top of movable disk
For to the inclined-plane of lopsidedness, inclined-plane arranges fluid channel, the tree fork shape being distributed as branch of described fluid channel, the horse in base
Reach by driving movable disk to carry out translational motion innermost being handle slide block structure.
Micro-fluidic type single cell separator the most according to claim 1, it is characterised in that: by solid bottom described movable disk
Determine plate to be connected with to innermost being handle slide block structure.
Micro-fluidic type single cell separator the most according to claim 1, it is characterised in that: on described inclined-plane, fluid channel is upper
Side arranges transparent cover plate.
Micro-fluidic type single cell separator the most according to claim 1, it is characterised in that: the material of described fluid channel is for having
Organic polymeric material.
Micro-fluidic type single cell separator the most according to claim 1, it is characterised in that: in described fluid channel, outflow end has
The guiding gutter of hollow.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410399750.0A CN104152345B (en) | 2014-08-14 | 2014-08-14 | Micro-fluidic type single cell separator |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410399750.0A CN104152345B (en) | 2014-08-14 | 2014-08-14 | Micro-fluidic type single cell separator |
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| Publication Number | Publication Date |
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| CN104152345A CN104152345A (en) | 2014-11-19 |
| CN104152345B true CN104152345B (en) | 2016-08-24 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105950461B (en) * | 2016-07-20 | 2017-10-24 | 江苏瑞明生物科技有限公司 | The unicellular accurate delivery system of nanometer |
| CN109920482A (en) * | 2019-01-29 | 2019-06-21 | 厦门大学 | A method of analyzing unicellular content |
| CN113322167B (en) * | 2021-04-26 | 2023-02-03 | 上海交通大学 | Micro-fluidic chip |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| TW201105971A (en) * | 2009-07-06 | 2011-02-16 | Sony Corp | Microfluidic device having onboard tissue or cell sample handling capability |
| CN103131693B (en) * | 2013-02-25 | 2014-12-24 | 宁波美晶医疗技术有限公司 | Separation method of rare cells in blood |
| CN203284394U (en) * | 2013-05-17 | 2013-11-13 | 宁波美晶医疗技术有限公司 | Pendulous device for blood detection biological chips |
| CN203976784U (en) * | 2014-08-14 | 2014-12-03 | 江苏瑞明生物科技有限公司 | micro-fluidic type single cell separator |
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