CN104117057B - Loaded enzyme hydrogel composition with oxygen releasing function and hydrogel prepared from hydrogel composition - Google Patents
Loaded enzyme hydrogel composition with oxygen releasing function and hydrogel prepared from hydrogel composition Download PDFInfo
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- CN104117057B CN104117057B CN201410326198.2A CN201410326198A CN104117057B CN 104117057 B CN104117057 B CN 104117057B CN 201410326198 A CN201410326198 A CN 201410326198A CN 104117057 B CN104117057 B CN 104117057B
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims abstract description 104
- 239000001301 oxygen Substances 0.000 title claims abstract description 102
- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 102
- 239000000017 hydrogel Substances 0.000 title claims abstract description 46
- 239000000203 mixture Substances 0.000 title claims abstract description 39
- 102000004190 Enzymes Human genes 0.000 title abstract description 7
- 108090000790 Enzymes Proteins 0.000 title abstract description 7
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 37
- 239000000084 colloidal system Substances 0.000 claims abstract description 20
- 239000003431 cross linking reagent Substances 0.000 claims abstract description 20
- 108010015776 Glucose oxidase Proteins 0.000 claims abstract description 17
- 235000019420 glucose oxidase Nutrition 0.000 claims abstract description 17
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 235000001727 glucose Nutrition 0.000 claims description 22
- 150000002304 glucoses Chemical class 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 8
- 229910021538 borax Inorganic materials 0.000 claims description 6
- 239000004328 sodium tetraborate Substances 0.000 claims description 6
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 5
- 239000004327 boric acid Substances 0.000 claims description 5
- 150000004680 hydrogen peroxides Chemical class 0.000 claims description 5
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims description 4
- 108010010803 Gelatin Proteins 0.000 claims description 4
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims description 4
- VTIIJXUACCWYHX-UHFFFAOYSA-L disodium;carboxylatooxy carbonate Chemical compound [Na+].[Na+].[O-]C(=O)OOC([O-])=O VTIIJXUACCWYHX-UHFFFAOYSA-L 0.000 claims description 4
- 239000008273 gelatin Substances 0.000 claims description 4
- 229920000159 gelatin Polymers 0.000 claims description 4
- 235000019322 gelatine Nutrition 0.000 claims description 4
- 235000011852 gelatine desserts Nutrition 0.000 claims description 4
- -1 polyethylene Polymers 0.000 claims description 4
- 229940045872 sodium percarbonate Drugs 0.000 claims description 4
- YKLJGMBLPUQQOI-UHFFFAOYSA-M sodium;oxidooxy(oxo)borane Chemical compound [Na+].[O-]OB=O YKLJGMBLPUQQOI-UHFFFAOYSA-M 0.000 claims description 4
- LUEWUZLMQUOBSB-FSKGGBMCSA-N (2s,3s,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-6-[(2r,3s,4r,5s,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5s,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](OC3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-FSKGGBMCSA-N 0.000 claims description 3
- 229920002907 Guar gum Polymers 0.000 claims description 3
- 241001597008 Nomeidae Species 0.000 claims description 3
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 3
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims description 3
- 239000000665 guar gum Substances 0.000 claims description 3
- 235000010417 guar gum Nutrition 0.000 claims description 3
- 229960002154 guar gum Drugs 0.000 claims description 3
- 235000010413 sodium alginate Nutrition 0.000 claims description 3
- 238000006555 catalytic reaction Methods 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims 3
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 1
- 239000004698 Polyethylene Substances 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 230000000474 nursing effect Effects 0.000 claims 1
- 229920000573 polyethylene Polymers 0.000 claims 1
- 239000000661 sodium alginate Substances 0.000 claims 1
- 229940005550 sodium alginate Drugs 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 9
- 239000003054 catalyst Substances 0.000 abstract description 8
- 239000008103 glucose Substances 0.000 abstract description 7
- 239000004366 Glucose oxidase Substances 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 6
- 229940088598 enzyme Drugs 0.000 abstract description 6
- 229940116332 glucose oxidase Drugs 0.000 abstract description 6
- 230000035876 healing Effects 0.000 abstract description 5
- 230000004060 metabolic process Effects 0.000 abstract description 4
- 230000017531 blood circulation Effects 0.000 abstract description 3
- 239000012567 medical material Substances 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract 1
- 208000027418 Wounds and injury Diseases 0.000 description 23
- 206010052428 Wound Diseases 0.000 description 21
- 230000000694 effects Effects 0.000 description 13
- 102000016938 Catalase Human genes 0.000 description 11
- 108010053835 Catalase Proteins 0.000 description 11
- 238000013019 agitation Methods 0.000 description 10
- 239000003643 water by type Substances 0.000 description 10
- 239000000499 gel Substances 0.000 description 9
- NUJOXMJBOLGQSY-UHFFFAOYSA-N manganese dioxide Chemical compound O=[Mn]=O NUJOXMJBOLGQSY-UHFFFAOYSA-N 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 230000029663 wound healing Effects 0.000 description 7
- 238000013268 sustained release Methods 0.000 description 6
- 239000012730 sustained-release form Substances 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- AQLJVWUFPCUVLO-UHFFFAOYSA-N urea hydrogen peroxide Chemical compound OO.NC(N)=O AQLJVWUFPCUVLO-UHFFFAOYSA-N 0.000 description 6
- 239000007864 aqueous solution Substances 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
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- 206010021143 Hypoxia Diseases 0.000 description 4
- 239000004372 Polyvinyl alcohol Substances 0.000 description 4
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- 229920002451 polyvinyl alcohol Polymers 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000010408 film Substances 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 230000007954 hypoxia Effects 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 101710128063 Carbohydrate oxidase Proteins 0.000 description 2
- 244000007835 Cyamopsis tetragonoloba Species 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 240000006365 Vitis vinifera Species 0.000 description 2
- 235000014787 Vitis vinifera Nutrition 0.000 description 2
- 235000010443 alginic acid Nutrition 0.000 description 2
- 239000000783 alginic acid Substances 0.000 description 2
- 229920000615 alginic acid Polymers 0.000 description 2
- 229960001126 alginic acid Drugs 0.000 description 2
- 150000004781 alginic acids Chemical class 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 2
- 235000002532 grape seed extract Nutrition 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 208000028867 ischemia Diseases 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 206010002660 Anoxia Diseases 0.000 description 1
- 241000976983 Anoxia Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 208000014770 Foot disease Diseases 0.000 description 1
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- 241000219095 Vitis Species 0.000 description 1
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- 229940088710 antibiotic agent Drugs 0.000 description 1
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- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 125000003051 glycosyloxy group Chemical group 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 239000000416 hydrocolloid Substances 0.000 description 1
- 238000002639 hyperbaric oxygen therapy Methods 0.000 description 1
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- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
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- 150000002978 peroxides Chemical class 0.000 description 1
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Abstract
The invention discloses a load enzyme hydrogel composition with oxygen releasing function and hydrogel prepared from the hydrogel composition, the the hydrogel composition contains a component A and a component B, wherein the active components of the component A are glucose oxidase, a catalyst for catalyzing hydrogen peroxide to produce oxygen, and hydrophilic colloid; the active components of the component B are glucose and a cross linking agent. The preparation method of the hydrogel is even mixing of the component A and the component B. The load enzyme hydrogel composition adopts raw materials belonging to the food or medical materials with high biological safety and good biocompatibility; and before use, the two components are not contacted and stable in performance. The hydrogel prepared by mixing of the hydrogel composition can continuously supply oxygen, can increase the oxygen partial pressure at the wound, improves the wound tissue oxygen supply, improves the local tissue aerobic metabolism, is conducive to healing, enables the oxygen to reach to the wound through the body outer surface other than blood circulation, is a local oxygen supply technology and easy to carry, and has wide popularization and application prospect.
Description
Technical field
The present invention relates to a kind of hydrogel composition with oxygen release function of load enzyme and its hydrogel of formation.
Background technology
With the development that wound healing is theoretical, the impact of the wound healing of oxygen has obtained increasing understanding.Such as sugar
The major reason that the chronic wounds such as urine foot disease are difficult to heal is that wound location blood supply scarcity causes oxygen to pass through
Blood is delivered to wound.And for example when the narrow deep, ischemia of wound, slough are more, inadequate drainage, and it is mixed with other aerobe infection
And when causing wound ischaemia, tetanus are just susceptible to.The effect that oxygen is played in wound healing process is many-sided
, wound healing is the process of a power consumption, and oxygen can provide the energy of process needs and support；In promotion organization regenerative process
The synthesis of collagen and the generation of new vesselses；Oxygen also plays very big effect, the presence energy of oxygen in the antibiosis of wound
Kill some antibacterials for producing pathogenic and stench.Although to a certain degree hypoxia can start the physiology repair process of body, not
Harmful effect of the anoxia to wound healing can be ignored：Hypoxia limits wound healing.Wound healing needs the blood by organization restructuring
Perfusion and oxygen supply.There is obvious benefit by improving oxygen and being provided with correcting healing of the hypoxia to wound for existing.
Oxygen in air is not high due to partial pressure of oxygen, and some wounds for processing often have covering, and these all have impact on oxygen
Gas gos deep into, therefore air oxygen is little to wound effect.One feasible method for supplying oxygen is hyperbaric oxygentherapy, and concrete grammar is to allow disease
People is entered in the storehouse of a closing, inhales 100% oxygen, and oxygen can be made to be dissolved in the increase of the amount in blood plasma, increases whole body oxygenation,
This is a kind of whole body oxygen-supplying technology.It is also a kind of effective manner using localized sustained blow oxygen after air exclusion, its principle is
Suppress wound surface anaerobism bacteria growing using pure oxygen, improve wound tissue oxygen supply, improve local organization aerobic metabolism, be conducive to healing.
This is to allow oxygen to pass through body exterior face rather than blood circulation arrival wound, is a kind of local oxygen supply technology.It is avoided that whole body
Some side effect of oxygen supply, and for patient, local oxygen supply feels pleasant, and is easy to carry.By some oxygen
Changing reduction reaction can also provide source of oxygen.But from from the point of view of bio-safety, great majority reaction simultaneously can not be adopted.Exploitation tool
Reactive pattern oxygen release function, low cost, the material of the oxygen being convenient for carrying or system are significantly.
The content of the invention
In order to solve above-mentioned problem, the present invention is prepared for a kind of compositionss, can generate after said composition mixing
Hydrogel with lasting oxygen delivery capacity, compositionss low cost prepared by the present invention, is convenient for carrying, and sustainable oxygen supply, with straight
Connect and skin histology contact, it is safe, it is very beneficial for the oxygen supply of wound tissue.
It is an object of the invention to provide generating the compositionss with oxygen release function hydrogel after a kind of mixing.
Another object of the present invention is to provide a kind of hydrogel with oxygen release function.
The technical solution used in the present invention is：
A kind of hydrogel composition with oxygen release function, said composition contains component A and B component, the wherein work of component A
Property composition be glucoseoxidase, catalyzing hydrogen peroxide generate oxygen catalyst, hydrophilic colloid；The active component of B component is
Glucose, cross-linking agent.
Further, above-mentioned composition contains component A and B component, and wherein component A contains 10～2000 g/mL glucoses
Oxidase, 10～2000 g/mL catalyzing hydrogen peroxides generate catalyst, 10～300g/L hydrophilic colloids of oxygen, balance of water；
B component contains 1～400g/L glucoses, 1～80g/L cross-linking agent, 0～10g/L hydrogen peroxide sources, balance of water.
Further, above-mentioned composition contains component A and B component, and wherein component A contains 500～1000 g/mL Fructus Vitis viniferaes
Carbohydrate oxidase, 500～1000 g/mL catalyzing hydrogen peroxides generate catalyst, 30～100g/L hydrophilic colloids of oxygen, balance of
Water；B component contains 50～150g/L glucoses, 10～40g/L cross-linking agent, 0～2g/L hydrogen peroxide sources, balance of water.
Further, above-mentioned hydrophilic colloid is selected from polyvinyl alcohol, guar gum and its derivant, gelatin, glucomannoglycan, Sargassum
At least one of sour sodium, Oxytarch, carboxymethyl cellulose.
Further, above-mentioned cross-linking agent is selected from Borax or boric acid.
Further, the one kind of above-mentioned hydrogen peroxide source in urea peroxide, Dexol, SODIUM PERCARBONATE.
A kind of hydrogel with oxygen release function, its preparation method be by the component A in above-mentioned composition and the mixing of B groups i.e.
Can, the mass ratio of wherein hydrophilic colloid and cross-linking agent is（2～20）:1.
The invention has the beneficial effects as follows：
1）Hydrogel prepared by the inventive method has biological safety, good biocompatibility.Two components are not connect using front
Touch, stable performance.
2）The polymer colloid type that the present invention is adopted belongs to foodstuff glue, and Borax or boric acid are also common medical material
Material.Therefore the gelling performance safety, directly can contact with skin histology, it is highly suitable for as wound tissue oxygen supply.
3）The sustainable oxygen supply of hydrogel formed after the compositionss mixing of the present invention, can increase the partial pressure of oxygen of wound, carry
High wound tissue oxygen supply, improves local organization aerobic metabolism, is conducive to healing.This be allow oxygen pass through body exterior face rather than
Blood circulation reaches wound, is a kind of local oxygen supply technology, is easy to carry.
Description of the drawings
Fig. 1 is the testing result of the release oxygen of the hydrogel generated after combined hybrid of the present invention；
Fig. 2 is the schematic diagram of the hydrogel in skin surface site of injury application of present composition formation（1 represents skin in figure
Skin, 2 represent gel, and 3 represent coverlay）.
Specific embodiment
A kind of hydrogel composition with oxygen release function, said composition contains component A and B component, the wherein work of component A
Property composition be glucoseoxidase, catalyzing hydrogen peroxide generate oxygen catalyst, hydrophilic colloid；The active component of B component is
Glucose, cross-linking agent.
Preferably, above-mentioned composition contains component A and B component, and wherein component A contains 10～2000 g/mL Fructus Vitis viniferae glycosyloxies
Change catalyst, 10～300g/L hydrophilic colloids that enzyme, 10～2000 g/mL catalyzing hydrogen peroxides generate oxygen, balance of water；B
Component contains 1～400g/L glucoses, 1～80g/L cross-linking agent, 0～10g/L hydrogen peroxide sources, balance of water.
It is furthermore preferred that above-mentioned composition contains component A and B component, wherein component A contains 500～1000 g/mL Fructus Vitis viniferaes
Carbohydrate oxidase, 500～1000 g/mL catalyzing hydrogen peroxides generate catalyst, 30～100g/L hydrophilic colloids of oxygen, balance of
Water；B component contains 50～150g/L glucoses, 10～40g/L cross-linking agent, 0～2g/L hydrogen peroxide sources, balance of water.
Preferably, above-mentioned glucose is nonspecific D-Glucose
Preferably, above-mentioned hydrophilic colloid is selected from polyvinyl alcohol, guar gum and its derivant, gelatin, glucomannoglycan, alginic acid
At least one of sodium, Oxytarch, carboxymethyl cellulose.
Preferably, above-mentioned cross-linking agent is selected from Borax or boric acid.
Preferably, the one kind of above-mentioned hydrogen peroxide source in urea peroxide, Dexol, SODIUM PERCARBONATE.
Preferably, above-mentioned catalyzing hydrogen peroxide generates the catalyst of oxygen selected from catalase, Fe3+In manganese dioxide
It is a kind of.
A kind of hydrogel with oxygen release function, its preparation method be by the component A in above-mentioned composition and the mixing of B groups i.e.
Can, the mass ratio of wherein hydrophilic colloid and cross-linking agent is（2～20）:1.
The compositionss and hydrogel of the present invention discharge the mechanism of oxygen：
Glucoseoxidase, catalase form the component A of hydrogel, Portugal together with hydrocolloid systems in the present invention
Grape sugar forms the B component of hydrogel together with cross-linking agent.Component A and B component are being mixed, and form gel after mixing at once
Material, the glucoseoxidase effect in the glucose diffusion of components in B component, with component A produces H2O2, the H of generation2O2Expand
Aproll close after with component A in catalase（Fe3+Or manganese dioxide）Effect, sustained release oxygen.May be used also in the present invention
To introduce other hydrogen peroxide sources in B component（Urea peroxide, Dexol, SODIUM PERCARBONATE）, other hydrogen peroxide sources and peroxide
Change the oxygen that hydrogen enzyme effect produces early stage, be more beneficial for improving the oxygen partial pressure of local rapidly, while the oxygen for producing is conducive to
Glucose produces H under glucoseoxidase catalysis2O2.The present invention can be toward skin histology conveying high-pressure oxygen, and gel is used during effect
The thin film of the autohension of oxygen flow is covered, and first other hydrogen peroxide sources such as urea peroxide can rapidly produce oxygen, empty covering
Between in form higher partial pressure of oxygen, while the oxygen of outside constantly can enter gel through oxygen permeation membrane, participate in glucose
Enzymatic oxidation reduction reaction, forms a lasting oxygen release process, and keeps certain partial pressure of oxygen.
It is double-component that the inventive method prepares hydrogel composition, is retained separately using front double-component, completely cuts off air.Use
When the system of double-component is sprayed onto on body surface, form one layer of hydrogel in skin at once, the self-adhesive foil of gel oxygen flow is covered.
An oxygen part for generation is dissolved in hydrogel, becomes dissolved oxygen, and most of gaseous oxygen forms high in the space that film is covered
Oxygen partial pressure.The dissolved oxygen and gaseous oxygen of generation is transmitted on skin by gel, can effectively improve the oxygen of local skin
Pressure.The hydrogen peroxide major part that the material of gel load is produced is quickly converted to oxygen, and also small part hydrogen peroxide can conduct
To skin histology, disinfection can be played a part of, because unconverted amount of hydrogen peroxide is less, without compromising on skin.
With reference to specific embodiment, the present invention is further illustrated, but is not limited thereto.
Embodiment 1
8g guar gums, heating for dissolving is added to be cooled to after room temperature and add 1.5g sodium alginates molten in 50mL deionized waters
Solution, adds under agitation 0.04g glucoseoxidases and 0.04g catalases, and mix homogeneously is formed sticky water-soluble
Liquid, i.e. component A（Containing 800 g/mL glucoseoxidases, 800 g/mL catalases, 190g/L hydrophilic colloids）；
0.8 gram of Borax, dissolving is added to add 6g glucoses under agitation in 50mL deionized waters, mixing is equal
Even formation aqueous solution, as B component（Containing 120g/L glucoses, 16g/L cross-linking agent）；
Component A and B component are mixed, hydrogel is obtained final product, determining it with oxygen electrode instrument has oxygen sustained release effect.A groups
Divide after mixing in closed flask with B component, oxygen concentration change therein is determined by oxygen concentration determining instrument, determine
As a result it is as shown in Figure 1.
Embodiment 2
0.3 g polyvinyl alcohol, heating for dissolving is added to be cooled to addition 0.2g alginic acid after room temperature in 50mL deionized waters
Sodium dissolves, and 0.1g glucoseoxidases and 0.1g catalases are added under agitation, and mix homogeneously forms sticky water
Solution, i.e. component A（Containing 2000 g/mL glucoseoxidases, 2000 g/mL catalases, 10g/L hydrophilic colloids）；
0.1 g Boraxs are added in 50mL deionized waters, is dissolved, add 0.5g glucoses under agitation, 0.5 gram
Urea peroxide, mix homogeneously forms aqueous solution, as B component（Containing 10g/L glucoses, 2g/L cross-linking agent）；
Component A and B component are mixed, hydrogel is obtained final product, determining it with oxygen electrode instrument has oxygen sustained release effect.
Embodiment 3
150g gelatin, heating for dissolving is added to be cooled to after room temperature and add 50g Oxytarch molten in 500mL deionized waters
Solution, adds under agitation 0.005g glucoseoxidases and 0.005g catalases, and mix homogeneously forms sticky water
Solution, i.e. component A（Containing 10 g/mL glucoseoxidases, 10 g/mL catalases, 300g/L hydrophilic colloids）；
40 g Boraxs are added in 500mL deionized waters, is dissolved, 200g glucoses are added under agitation, mixed
It is formed uniformly aqueous solution, as B component（Containing 400g/L glucoses, 80g/L cross-linking agent）；
Component A and B component are mixed, hydrogel is obtained final product, determining it with oxygen electrode instrument has oxygen sustained release effect.
Embodiment 4
1.3g guar gums, heating for dissolving is added to be cooled to addition 0.2g sodium alginates after room temperature in 50mL deionized waters
Dissolving, adds under agitation 0.025g glucoseoxidases and 0.025g manganese dioxide, and mix homogeneously forms sticky water
Solution, i.e. component A（Containing 500 g/mL glucoseoxidases, 500 g/mL catalases, 30g/L hydrophilic colloids）；
0.5 gram of Borax, dissolving is added to add 2.5g glucoses and 0.1g under agitation in 50mL deionized waters
Urea peroxide, mix homogeneously forms aqueous solution, as B component（Containing 50g/L glucoses, 10g/L cross-linking agent, 2g/L peroxidating
Hydrogen source）；
Component A and B component are mixed, hydrogel is obtained final product, determining it with oxygen electrode instrument has oxygen sustained release effect.
Embodiment 5
4g polyvinyl alcohol, heating for dissolving is added to be cooled to addition 1g carboxymethyl celluloses after room temperature in 50mL deionized waters
Element dissolving, adds under agitation 0.05g glucoseoxidases and 0.05g manganese dioxide, and mix homogeneously forms sticky water
Solution, i.e. component A（Containing 1000 g/mL glucoseoxidases, 1000 g/mL catalases, 100g/L hydrophilic colloids）；
2 grams of boric acid, dissolving are added to add 7.5g glucoses and 0.5g mistakes under agitation in 50mL deionized waters
Sodium carbonate, mix homogeneously forms aqueous solution, as B component（Containing 150g/L glucoses, 40g/L cross-linking agent, 10g/L peroxidating
Hydrogen source）.
Component A and B component are mixed, hydrogel is obtained final product, determining it with oxygen electrode instrument has oxygen sustained release effect.
Application of the compositionss that below prepared by Example 1 in surface injury.
The component A and B component by volume 10 of compositionss prepared by Example 1：1 is sprayed on same skin surface wound
Place（1）Form hydrogel（2）, with the film of one layer of autohension（3）Cover, schematic diagram as shown in Figure 2, the oxidation of gel load is also
Substance system reacts, and produces oxygen, and partial high pressure oxygen and dissolved oxygen are produced on the skin histology 1 that film is covered, and toward skin
Tissue infiltration.
The sustainable oxygen supply of hydrogel formed after the compositionss mixing of the present invention, can increase the partial pressure of oxygen of wound, improve
Wound tissue oxygen supply, improves local organization aerobic metabolism, is conducive to healing.This is to allow oxygen to pass through body exterior face rather than blood
Liquid cycles to reach wound, is a kind of local oxygen supply technology, is easy to carry.
Claims (7)
1. a kind of hydrogel composition with oxygen release function, it is characterised in that：Said composition contains component A and B component, wherein
Component A contains the catalysis that 500～1000 g/mL glucoseoxidases, 500～1000 g/mL catalyzing hydrogen peroxides generate oxygen
Agent, 30～100g/L hydrophilic colloids, balance of water；B component contains 50～150g/L glucoses, 10～40g/L cross-linking agent, 0～
2g/L hydrogen peroxide sources, balance of water；The one kind of described hydrogen peroxide source in Dexol, SODIUM PERCARBONATE.
2. according to the arbitrary described hydrogel composition of claim 1, it is characterised in that：Described hydrophilic colloid is selected from polyethylene
At least one of alcohol, guar gum and its derivant, gelatin, glucomannoglycan, sodium alginate, Oxytarch, carboxymethyl cellulose.
3. according to the arbitrary described hydrogel composition of claim 1, it is characterised in that：Described cross-linking agent selected from Borax or
Boric acid.
4. a kind of hydrogel with oxygen release function, it is characterised in that：Its preparation method is that claims 1 to 3 is arbitrary described
Component A and B groups in hydrogel composition is mixed, and the mass ratio of wherein hydrophilic colloid and cross-linking agent is（2～20）:1.
5. application of the arbitrary described hydrogel composition of Claims 1 to 4 in skin nursing medicine is prepared.
6. the arbitrary described hydrogel composition of Claims 1 to 4 is preparing the application conveyed to skin in the medicine of oxygen.
7. hydrogel described in claim 5 is preparing the application conveyed to skin in the medicine of oxygen.
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| EP3915552A1 (en) * | 2015-06-01 | 2021-12-01 | Xeno Biosciences Inc. | Compositions for use in modulating the gut microbiota and managing weight |
| CN107961114A (en) * | 2017-12-19 | 2018-04-27 | 大连海事大学 | A kind of wound dressing and its production method based on solid particle oxygen supply |
| CN108660184A (en) * | 2018-05-25 | 2018-10-16 | 郑州安图生物工程股份有限公司 | The test scraps of paper of catalase in a kind of detection bacterium |
| CN110478525B (en) * | 2019-07-26 | 2021-12-07 | 广东省医疗器械研究所 | Self-crosslinking hydrogel with synergistic effect of sugar and enzyme and preparation method thereof |
| CN111116942B (en) * | 2019-12-30 | 2023-05-30 | 广东省医疗器械研究所 | Controllable self-crosslinking oxygen-releasing hydrogel composition and application thereof |
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