CN104095692A - Method for manufacturing bovine jugular vein valved conduit - Google Patents

Method for manufacturing bovine jugular vein valved conduit Download PDF

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Publication number
CN104095692A
CN104095692A CN201310118841.8A CN201310118841A CN104095692A CN 104095692 A CN104095692 A CN 104095692A CN 201310118841 A CN201310118841 A CN 201310118841A CN 104095692 A CN104095692 A CN 104095692A
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China
Prior art keywords
vessel
blood vessel
jugular vein
bovine
method
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CN201310118841.8A
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Chinese (zh)
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CN104095692B (en
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李小静
汪胜
郑扬
王铭
张晰斌
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天津市塑料研究所有限公司
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Abstract

The invention relates to an improved method for manufacturing a medical bovine jugular vein valved conduit. The bovine jugular vein valved conduit needs to be fixed before being implanted into human body. A fixing treatment method is improved. The method is characterized in that during fixing, a tube cavity is fully filled with fixing liquid, and the conduit is soaked into a container containing the fixing liquid after the two tail ends of blood vessel are ligated. The method has the advantages that the valved conduit can be fixed fully to better enhance the tissue strength and durability; moreover, the original shape of blood vessel can be well kept, wrinkling, twisting and knotting are avoided, convenience is brought to anastomosis in surgeries, pain of a patient and trouble of an operator are reduced, the surgical risk is lowered, and the use safety is improved.

Description

牛颈静脉带瓣管道的制作方法 Production methods bovine jugular vein conduit of

技术领域 FIELD

[0001] 本专利属于利用医用生物材料的制造技术领域,尤其涉及一种牛颈静脉带瓣管道的制作方法。 [0001] This patent belongs to the technical field of manufacturing a medical use of biological material, in particular, it relates to a process for the production of bovine jugular vein conduit.

背景技术 Background technique

[0002] 先心病发病率居出生缺陷的首位。 [0002] The incidence of congenital heart disease ranks first in birth defects. 先心病约有1/3的患者存在不同程度的右室或左室流出道梗阻,其中相当一部分需要应用带瓣管道或带瓣血管片重建右心室流出道方能获得根治,如法乐四联症、右室双出口、大血管转位中的严重肺动脉狭窄、肺动脉闭锁或共同动脉干等。 Congenital heart disease, about a third of patients present with varying degrees of left ventricular or right ventricular outflow tract obstruction, a considerable portion of which needs to be applied valved conduit or valved vascular reconstruction slice in order to obtain the right ventricular outflow tract radical, such as tetralogy quadruple syndrome, double outlet right ventricle, great vessels in severe pulmonary stenosis, pulmonary atresia truncus arteriosus, or the like. 常用的材料有人工合成的异种带瓣管道、同种异体主动脉带瓣管道、同种带瓣管道,但这些材料均存在局限性,如异种管道容易钙化、内膜增生;同种管道来源困难,植入宿主体内仍有轻度排斥反应,在矫治婴幼儿病变时大小不易匹配。 Materials commonly used synthetic heterogeneous valved conduit, allograft aortic valved conduit, valved conduit, but these materials have limitations, such as calcification readily dissimilar pipe, intimal hyperplasia; isoforms difficult conduit source implantation within the host still mild rejection, when the treatment is not easy to match the size of the lesion infants. 牛颈静脉具备来源丰富、长度足够等优点,目前被认为是一种非常具有研究前途的生物材料。 Bovine jugular vein with a rich source of sufficient length, etc., is now considered a very promising research with biological material. 但在制作过程中与其他异种材料一样,都需要经过固定处理,以增加组织强度和耐久性,降低抗原性和免疫原性。 However, in the production process as other dissimilar materials, we have to go through the fixing process, the organization in order to increase strength and durability, reduce antigenicity and immunogenicity. 传统固定操作是将采集修剪好的血管用清水、生理盐水、平衡盐溶液溶液清洗干净后, 直接放到固定液中进行固定,这种方法不能保证带瓣管道固定充分,同时无法保证带瓣管道原有形状,不利于手术的吻合,不能保证产品的质量,给患者生命带来风险。 Traditional fixing operation is a good collection vessel trimmed with water, physiological saline, balanced salt solution, after the wash solution, directly into fixative for fixing, this method does not guarantee sufficient valved conduit fixed, and no assurance valved conduit original shape is not conducive anastomosis can not guarantee the quality of the product, to the lives of patients at risk.

发明内容 SUMMARY

[0003] 本专利为解决公知技术中存在的技术问题而提供一种牛颈静脉带瓣管道的制作方法,该方法既保证了血管内外固定完全,又保证了血管的天然形状,不褶皱,不扭曲打结, 利于手术的吻合。 [0003] The present patent is to solve the technical problem known in the art exists to provide a method for manufacturing a bovine jugular vein conduit, the method not only ensures that the inner and outer vessels fixed completely, but also to ensure the natural shape of the blood vessel, no wrinkles, no twist tie, which will help anastomosis.

[0004] 本专利为解决公知技术中存在的技术问题所采取的技术方案是: [0004] This patent is a known technique to solve the technical problem of the present technical solution is adopted:

[0005] -种牛颈静脉带瓣管道的制作方法,包括以下步骤, [0005] - Production Method cattle jugular vein conduit, comprising the steps of,

[0006] A.选择牛源:选择牛龄2〜5岁、健康、无传染病的牛群为牛源; [0006] A. Selection of cattle Source: Selection of cattle aged 2 ~ 5 years old, healthy, non-infectious diseases of cattle is bovine;

[0007] B.采集血管:用刀划开牛颈部脂肪层并寻找到颈静脉血管,抓住血管用刀顺血管划向心脏处,取下的血管在1分钟内转入清洗; [0007] B. collection vessel: a knife to open the neck fat layer and bovine jugular vein to find, grasp the vessel to the heart draw knife along the blood vessel, the blood vessel into the washing removed in 1 minute;

[0008] C.清洗:用清水反复冲洗,洗掉血色及其他的污染物,将洗净的血管放入盛有4°C〜10°C的生理盐水的冰桶中临时贮存,贮存的时间控制在3小时内; [0008] C. washing: washed repeatedly with water, blood and other contaminants wash, wash the vessel into an ice bucket filled with physiological saline 4 ° C~10 ° C in temporary storage, the storage time control over 3 hours;

[0009] D.初选、清理:用手术剪将血管表面的脂肪和结蒂组织剪去,将病变、有伤痕的血管等淘汰; [0009] D. primaries, cleaning: with surgical scissors fat surface of blood vessels and connective tissue cut, the lesions, scars and blood vessels eliminated;

[0010] E.漂洗:将平衡盐溶液倒入盛有血管的容器中,用搅拌棒轻轻搅动,使血管均匀散开,每隔1小时换一次液体,共换平衡盐溶液6次,然后用生理盐水再次清洗,具体操作与C步骤中的清洗部分相同; [0010] E. Rinse: The balanced salt solution was poured into a vessel filled with blood vessel, gently stir with a stirring rod, a uniform spread of the blood vessel, a liquid hours for once, a total of six times change every balanced salt solution, and then washed again with saline, washing the same with some of the specific operation of the step (C);

[0011] F.固定:先将血管一侧用医用缝合线结扎,将预先配制好的0.6%戊二醛固定液灌入管腔内,灌满后结扎另一侧,再将血管放入盛有0.6%戊二醛固定液的容器中固定,温度保持在4-12°C ;48小时后再将血管转入到0.3%戊二醛固定液中,固定一周,备用; [0011] F. fixed: a first side vascular surgical suture ligation, the previously prepared 0.6% glutaraldehyde fixative good poured into the lumen, the other side of the rear filled ligation, and then filled into the vessel 0.6% glutaraldehyde fixative container is fixed, the temperature was maintained at 4-12 ° C; 48 hours after the vessel into 0.3% glutaraldehyde fixative fixed week standby;

Claims (2)

1. 一种牛颈静脉带瓣管道的制作方法,其特征是:包括以下步骤, A. 选择牛源:选择牛龄2〜5岁、健康、无传染病的牛群为牛源; B. 采集血管:用刀划开牛颈部脂肪层并寻找到颈静脉血管,抓住血管用刀顺血管划向心脏处,取下的血管在1分钟内转入清洗; C. 清洗:用清水反复冲洗,洗掉血色及其他的污染物,将洗净的血管放入盛有4°C〜 10°C的生理盐水的冰桶中临时贮存,贮存的时间控制在3小时内; D. 初选、清理:用手术剪将血管表面的脂肪和结蒂组织剪去,将病变、有伤痕的血管等淘汰; E. 漂洗:将平衡盐液倒入盛有血管的容器中,用搅拌棒轻轻搅动,使血管均匀散开,每隔1小时换一次液体,共换平衡盐溶液6次,然后用生理盐水再次清洗,具体操作与C步骤中的清洗部分相同; F. 固定:先将血管一侧用医用缝合线结扎,将预先配制好的0.6%戊 1. A method of making bovine jugular vein conduit, characterized in that: comprising the steps of, A. bovine selection: Select the cow 2 ~ 5 years old, healthy and free of infectious bovine cattle; B. collection vessel: a knife to open the neck fat layer and bovine jugular vein to find, grasp the vessel to the heart draw knife along the blood vessel, the blood vessel into the washing removed within one minute; C. cleaning: water repeatedly washing, color wash, and other contaminants will be washed into the vessel filled with physiological saline 4 ° C~ 10 ° C in an ice bucket temporary storage, storage time control over 3 hours; D. primaries , cleaning: with surgical scissors fat surface of blood vessels and connective tissue cut, lesion, bruises and the like out of the vessel; E. rinse: the balanced salt solution poured into a container having a blood vessel, with a stirring rod gently agitation, the blood vessel spread evenly, the liquid for once every 1 hour, a total of six times transducer balanced salt solution, and then washed again with saline, washing the same with some of the specific operation of the step (C); F. fixed: a first vessel side ligated with surgical suture, the pre-formulated pent 0.6% 醛固定液灌入管腔内,灌满后结扎另一侧,再将血管放入盛有0.6%戊二醛固定液的容器中固定,温度保持在4-12°C ;48小时后再将血管转入到0.3%戊二醛固定液中,固定一周,备用; G. 包涤纶布:用宽度为25mm的涤纶布缝制成筒状,其内径应与血管在自然松弛状态直径相同,并将其套在血管的瓣膜位置上,在其上下端口与血管表层围绕各缝合一周,在缝合时不能刺穿血管,并保持血管的自然松弛状态。 Aldehyde fixative poured into the lumen, the other side of the ligation filled, then placed in a blood vessel filled with 0.6% glutaraldehyde fixative container is fixed, the temperature was maintained at 4-12 ° C; 48 hours after the vessel into 0.3% glutaraldehyde fixative fixed week standby; G. polyester fabric bag: polyester fabric with a width of 25mm is sewn into a tubular shape, the inner diameter should be the same diameter as the vessel in a natural relaxed state, and which sets the valve position in the blood vessel, around the upper and lower surface of each port and vascular suture one week, the suture can not pierce the vessel, and maintain the natural state of the blood vessel relaxation.
2. 根据权利要求1所述的牛颈静脉带瓣管道的制作方法,其特征是:所述A选择牛源的步骤选用圈养水牛。 The manufacturing method according to a bovine jugular vein conduit claim, wherein: said step A of selecting selection captive bovine buffalo.
CN201310118841.8A 2013-04-08 2013-04-08 Production methods bovine jugular vein conduit of CN104095692B (en)

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RU2633544C1 (en) * 2016-06-06 2017-10-13 федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр имени академика Е.Н. Мешалкина" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ им. ак. Е.Н. Мешалкина" Минздрава России) Method for manufacture of valve-containing conduit from cattle jugular vein

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Publication number Priority date Publication date Assignee Title
RU2633544C1 (en) * 2016-06-06 2017-10-13 федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр имени академика Е.Н. Мешалкина" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ им. ак. Е.Н. Мешалкина" Минздрава России) Method for manufacture of valve-containing conduit from cattle jugular vein

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