CN104015306B - PCR plate injection forming mold and method thereof - Google Patents
PCR plate injection forming mold and method thereof Download PDFInfo
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- CN104015306B CN104015306B CN201410252512.7A CN201410252512A CN104015306B CN 104015306 B CN104015306 B CN 104015306B CN 201410252512 A CN201410252512 A CN 201410252512A CN 104015306 B CN104015306 B CN 104015306B
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- die cavity
- pcr plate
- glue
- feeder
- reaction tube
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C45/00—Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mould; Apparatus therefor
- B29C45/17—Component parts, details or accessories; Auxiliary operations
- B29C45/26—Moulds
Abstract
nullThe present invention relates to PCR plate injection molding technology field，Particularly relate to PCR plate injection forming mold and method thereof，Include upper mold、Lower mold，Formed between upper mold and lower mold and there is panel die cavity and the PCR plate die cavity of multiple reaction tube die cavity，Multiple glue-feeder is offered in upper mold，The bottom of each glue-feeder connects with panel die cavity upper end respectively，During injection mo(u)lding，The plastic material of molten condition is injected in PCR plate die cavity by the glue-feeder of panel die cavity upper end，Make plastic material be full of PCR plate die cavity and form PCR plate，The mouth of a river of PCR plate is positioned at the inner side of panel，And on reaction tube, there is no the mouth of a river，Improve product surface quality and performance，Reaction tube smooth surface is neat，Dimensionally stable，Translucent effect is good，Can the example reaction situation in observing response pipe well，Improve Product checking effect，And glue-feeder is fewer needed for mould，Simplify mould structure，Production efficiency is high，Water port of product is also few，Production cost is low，Conservation.
Description
Technical field:
The present invention relates to PCR plate injection molding technology field, particularly relate to PCR plate injection
Mould and method thereof.
Background technology:
At present, during molecular biology research and medical clinic applications, PCR reacts
Technology is indispensable.PCR reaction i.e. polymerase chain reaction (polymerase chain
Reaction, PCR), it is a kind of specific gene of rapid amplifying in vitro or the side of DNA sequence
Method, therefore it is also called the amplification in vitro method of gene.DNA segment both sides to be amplified and with
Two oligonucleotide primers that its both sides are complementary, through degeneration, anneal and extend several circulations
After, DNA cloning 2 square time times.In order to batch expands, PCR reaction is commonly used
PCR Sptting plate, as shown in accompanying drawing 1～3, PCR Sptting plate includes panel 1 ' with multiple
Reaction tube 2 ', PCR Sptting plate is usually 8 holes, 12 holes, 96 holes or 384 holes,
I.e. corresponding reaction tube 2 ' is 8,12,96 or 384, is exclusively for batch
Reaction designing, for PCR instrument and sequenator.
Existing PCR Sptting plate when injection mo(u)lding, typically PCR Sptting plate is inverted into
Row injection mo(u)lding, injection forming mold includes the lower mold that upper mold matches with upper mold, under
Mould is arranged on upper mold lower surface, forms PCR plate die cavity, PCR plate between upper mold and lower mold
Die cavity has panel die cavity 5 ' and multiple reaction tube die cavities being upside down in panel die cavity 5 ' upper end
6 ', when entering glue, it is typically all and is respectively provided with one at the top of each reaction tube die cavity 6 '
Glue-feeder 7 ', injects raw material to PCR plate die cavity by each glue-feeder 7 ' simultaneously, but
It is to be formed with the mouth of a river bottom each reaction tube of the PCR plate so produced, reaction
Pipe lower surface is rough, irregular, and size is unstable, and affect light penetrates effect,
The example reaction situation being unfavorable in observing response pipe, affects Detection results, and is molded into
The glue-feeder that mould needs is the most, and mould structure is complicated, and production efficiency is low, produces into
This height, wastes raw material.
Summary of the invention:
The purpose of the present invention is aiming at the deficiency of prior art existence and provides one to carry
High product surface quality and performance, the PCR plate injection forming mold of raising injection efficiency, also
The PCR plate injection molding forming method of this mould is provided.
To achieve these goals, the technical solution used in the present invention is:
PCR plate injection forming mold, includes the lower mold that upper mold matches with upper mold, under
Mould is arranged on upper mold lower surface, forms PCR plate die cavity, PCR plate between upper mold and lower mold
Die cavity has panel die cavity and multiple reaction tube die cavity being upside down in panel die cavity upper end, upper mold
On offer multiple glue-feeder, the bottom of each glue-feeder connects with panel die cavity upper end respectively.
The negative pressure line connected with extraneous air compressibility, negative pressure is offered in described upper mold
Pipeline top with each reaction tube die cavity respectively connects.
Each glue-feeder is respectively distributed in the space between adjacent multiple reaction tube die cavity.
The top of each glue-feeder is interconnected.
Multiple reaction tube die cavities are arranged in a matrix fashion.
The invention also discloses PCR plate injection molding forming method, comprise the following steps:
A, in mould formed PCR plate die cavity, make PCR plate die cavity have panel type
Chamber and multiple reaction tube die cavity being upside down in panel die cavity upper end；
B, the plastic material of molten condition is injected in PCR plate die cavity by panel die cavity upper end,
Make plastic material be full of PCR plate die cavity and form PCR plate.
Before carrying out described step B, produce negative inspiratory pressure shape first with air compression system
State, by the top of each reaction tube die cavity by the air sucking-off in PCR plate die cavity, makes PCR
Template intracavity forms negative pressure.
The negative pressure line connected with extraneous air compressibility is offered in advance in mould,
The negative pressure line top respectively with each reaction tube die cavity is made to connect, will by negative pressure line
Air sucking-off in PCR plate die cavity.
In described step B, in mould, offer multiple glue-feeder in advance, make each enter
The bottom of Jiao Kou connects with panel die cavity upper end respectively, by each glue-feeder simultaneously to PCR
Plastic material is injected in template chamber.
Each glue-feeder is respectively distributed in the space between adjacent multiple reaction tube die cavity.
The present invention has the beneficial effects that: the injection forming mold of the present invention include upper mold and
The lower mold that upper mold matches, lower mold is arranged on upper mold lower surface, shape between upper mold and lower mold
Becoming PCR plate die cavity, PCR plate die cavity has panel die cavity and multiple is upside down on panel die cavity
The reaction tube die cavity of end, upper mold offers multiple glue-feeder, and the bottom of each glue-feeder is divided
Do not connect with panel die cavity upper end, during injection mo(u)lding, by the plastic material of molten condition by face
The glue-feeder of upper end, template chamber injects in PCR plate die cavity, makes plastic material be full of PCR plate
Die cavity forms PCR plate, and the mouth of a river of prepared PCR plate is positioned at the inner side of panel, and reacts
Not having the mouth of a river on pipe, improve product surface quality and performance, reaction tube smooth surface is neat,
Dimensionally stable, translucent effect is good, it is possible to the example reaction situation in observing response pipe well,
Improve Product checking effect, and needed for mould, glue-feeder be fewer, simplify mould structure,
Production efficiency is high, and water port of product is also few, and production cost is low, conservation.
Accompanying drawing illustrates:
Fig. 1 is the structural representation of existing PCR plate.
Fig. 2 is the injection mo(u)lding schematic diagram of existing PCR plate.
Fig. 3 is the glue-feeder distribution schematic diagram of existing PCR plate.
Fig. 4 is the structural representation of injection forming mold of the present invention.
Fig. 5 is the enlarged drawing in Fig. 4 at A.
Fig. 6 is the injection mo(u)lding schematic diagram of PCR plate of the present invention.
Fig. 7 is the glue-feeder distribution schematic diagram of the PCR plate utilizing the present invention to prepare.
Detailed description of the invention:
Below in conjunction with the accompanying drawings and preferred embodiment the invention will be further described, see Fig. 4～7
Shown in, PCR plate includes panel 1 and multiple reaction tube 2, PCR plate injection forming mold
Including the lower mold 4 that upper mold 3 matches with upper mold 3, lower mold 4 is arranged on upper mold 3 lower end
On face, forming PCR plate die cavity between upper mold 3 and lower mold 4, PCR plate die cavity has panel
Die cavity 5 and multiple reaction tube die cavity 6 being upside down in panel die cavity 5 upper end, multiple reaction casts
Arranging in a matrix fashion in chamber 6, upper mold 3 offers multiple glue-feeder 7, each glue-feeder 7
Bottom connect with panel die cavity 5 upper end respectively.
The negative pressure line 8 connected with extraneous air compressibility, negative tube is offered in upper mold 3
Top with each reaction tube die cavity 6 respectively, road 8 connects, and utilizes air compression system to produce
Negative inspiratory pressure state, by the top of each reaction tube die cavity 6 by the air in PCR plate die cavity
Sucking-off, forms negative pressure, thus is attracted by panel die cavity 5 by raw material in making PCR plate die cavity
Ascending in reaction tube die cavity 6, injection moulding plastic raw material can be rapidly filled with reaction tube die cavity 6
With panel type chamber 5, injection efficiency is high, and avoids oxygen in PCR plate die cavity former with plastic cement
Material produces reaction makes plastic material burn, and improves performance and surface quality, the energy of plastic cement products
It is enough that injection molding is more accurate, size is less and the product of thin wall type；It addition, the present invention is also
Air compression system malleation blowing method can be utilized to be blown out by product, prevent product to be bonded at
On mould 3, preventing deformation of products and damage, the demoulding is easy.
Each glue-feeder 7 is respectively distributed in the space between adjacent multiple reaction tube die cavity 6,
Raw material can be rapidly introduced into each of surrounding by glue-feeder 7 after being entered in panel die cavity 5
In reaction tube die cavity 6, each glue-feeder 7 can supply to multiple reaction tube die cavities 6 simultaneously
Material, greatly reduces glue-feeder 7 quantity, simplifies mould structure, and production efficiency is high, product
The mouth of a river is also few, and production cost is low, conservation.The top of each glue-feeder 7 is interconnected,
Conveniently enter glue simultaneously.
During injection mo(u)lding, the plastic material of molten condition is entered glue by panel die cavity 5 upper end
Mouth 7 injects in PCR plate die cavities, makes plastic material be full of PCR plate die cavity and forms PCR plate,
The mouth of a river of the PCR plate prepared is positioned at the inner side of panel, and does not has the mouth of a river on reaction tube, improves
Product surface quality and performance, reaction tube smooth surface is neat, dimensionally stable, translucent effect
Good, it is possible to the example reaction situation in observing response pipe well, improve Product checking effect,
And glue-feeder is fewer needed for mould, simplifying mould structure, production efficiency is high, product water
Mouth is also few, and production cost is low, conservation.
The invention also discloses PCR plate injection molding forming method, comprise the following steps:
One, in mould, form PCR plate die cavity, make PCR plate die cavity have panel
Die cavity 5 and multiple reaction tube die cavity 6 being upside down in panel die cavity 5 upper end.
Two, air compression system is utilized to produce negative inspiratory pressure state, by each reaction tube die cavity 6
Top by the air sucking-off in PCR plate die cavity, make to be formed in PCR plate die cavity negative pressure.
Wherein, in mould, offer the negative pressure connected with extraneous air compressibility in advance
Pipeline 8, makes the negative pressure line 8 top respectively with each reaction tube die cavity 6 connect, by negative
Pressure pipe road 8 is by the air sucking-off in PCR plate die cavity.
Three, by the plastic material of molten condition by injection face template chamber, panel die cavity 5 upper end 5
In, the reaction tube die cavity 6 under negative pressure state can be by raw material by attraction in panel die cavity 5
Rise and enter in reaction tube die cavity 6, make plastic material be rapidly filled with PCR plate die cavity and form PCR
Plate, injection efficiency is high, and avoids oxygen in PCR plate die cavity to produce reaction with plastic material
Make plastic material burn, improve performance and the surface quality of plastic cement products, it is possible to injection molding
Comparison is the most accurate, size is less and the product of thin wall type；It addition, the present invention also is able to utilize sky
Product is blown out by air pressure compression system malleation blowing method, prevents product to be bonded in upper mold 3, prevents
Only deformation of products and damage, the demoulding is easy.
Wherein, offering multiple glue-feeder 7 in advance in mould, each glue-feeder 7 is respectively
It is distributed in the space between adjacent multiple reaction tube die cavity 6, makes the end of each glue-feeder 7
Portion connects with panel die cavity 5 upper end respectively, by each glue-feeder 7 simultaneously to PCR plate type
Plastic material is injected in chamber, conveniently enters glue simultaneously.
The mouth of a river of PCR plate utilizing the inventive method to prepare is positioned at the inner side of panel, and reacts
Not having the mouth of a river on pipe, improve product surface quality and performance, reaction tube smooth surface is neat,
Dimensionally stable, translucent effect is good, it is possible to the example reaction situation in observing response pipe well,
Improve Product checking effect, and needed for mould, glue-feeder be fewer, simplify mould structure,
Production efficiency is high, and water port of product is also few, and production cost is low, conservation.
Certainly, the above is only presently preferred embodiments of the present invention, therefore all according to patent of the present invention
The equivalence that structure, feature and principle described in application range is done changes or modifies, and all includes
In the range of present patent application.
Claims (7)
1.PCR plate injection moulding mould, includes the lower mold that upper mold matches with upper mold,
Lower mold is arranged on upper mold lower surface, forms PCR plate die cavity, PCR between upper mold and lower mold
Template chamber has panel die cavity and multiple reaction tube die cavity being upside down in panel die cavity upper end, its
Being characterised by: offer multiple glue-feeder in described upper mold, the bottom of each glue-feeder is respectively
Connect with panel die cavity upper end, upper mold offers connect with extraneous air compressibility negative
Pressure pipe road, negative pressure line top with each reaction tube die cavity respectively connects.
PCR plate injection forming mold the most according to claim 1, it is characterised in that:
Each glue-feeder is respectively distributed in the space between adjacent multiple reaction tube die cavity.
PCR plate injection forming mold the most according to claim 1, it is characterised in that:
The top of each glue-feeder is interconnected.
PCR plate injection forming mold the most according to claim 1, it is characterised in that:
Multiple reaction tube die cavities are arranged in a matrix fashion.
5.PCR plate injection moulding method, it is characterised in that comprise the following steps:
A, in mould formed PCR plate die cavity, make PCR plate die cavity have panel type
Chamber and multiple reaction tube die cavity being upside down in panel die cavity upper end；
B, first with air compression system produce negative inspiratory pressure state, by each reaction tube die cavity
Top by the air sucking-off in PCR plate die cavity, make to be formed in PCR plate die cavity negative pressure；
The plastic material of molten condition is injected in PCR plate die cavity by panel die cavity upper end, makes plastic cement
Raw material is full of PCR plate die cavity and forms PCR plate；
In step B, in mould, offer multiple glue-feeder in advance, make each glue-feeder
Bottom connect with panel die cavity upper end respectively, by each glue-feeder simultaneously to PCR plate type
Plastic material is injected in chamber.
PCR plate injection molding forming method the most according to claim 5, it is characterised in that:
In mould, offer the negative pressure line connected with extraneous air compressibility in advance, make to bear
Top with each reaction tube die cavity respectively, pressure pipe road connects, by negative pressure line by PCR plate
Air sucking-off in die cavity.
PCR plate injection molding forming method the most according to claim 5, it is characterised in that:
Each glue-feeder is respectively distributed in the space between adjacent multiple reaction tube die cavity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410252512.7A CN104015306B (en) | 2014-06-09 | 2014-06-09 | PCR plate injection forming mold and method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410252512.7A CN104015306B (en) | 2014-06-09 | 2014-06-09 | PCR plate injection forming mold and method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104015306A CN104015306A (en) | 2014-09-03 |
| CN104015306B true CN104015306B (en) | 2016-08-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410252512.7A Active CN104015306B (en) | 2014-06-09 | 2014-06-09 | PCR plate injection forming mold and method thereof |
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| CN (1) | CN104015306B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2561356B (en) * | 2017-04-10 | 2021-02-24 | Abgene Ltd | Overflow moulding apparatus and method |
| CN111251553A (en) * | 2020-02-25 | 2020-06-09 | 英普亿塑胶电子(苏州)有限公司 | PCR plate mold and processing method |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7347977B2 (en) * | 2000-06-08 | 2008-03-25 | Eppendorf Ag | Microtitration plate |
| DE102010008036A1 (en) * | 2010-02-05 | 2011-08-11 | Eppendorf AG, 22339 | microtiter plate |
| CN103302813B (en) * | 2013-06-20 | 2016-03-09 | 东莞市积健生物科技有限公司 | Plastic cement products negative pressure injection forming mold and method thereof |
| CN203973929U (en) * | 2014-06-09 | 2014-12-03 | 钟灿秋 | Pcr plate injection moulding mould |
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| CN104015306A (en) | 2014-09-03 |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
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| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20151008 Address after: 523000 Guangdong city of Dongguan province Shijie Zhen Shui Nan Chen Wu Cun industrial area C C02 Applicant after: DONGGUAN JIJIAN BIOTECHNOLOGY Co.,Ltd. Address before: 523000, Guangdong, Dongguan province Shijie Town Road, Chung Chen Industrial Zone bell Sheng mold factory Applicant before: Zhong Canqiu |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: No. 49, Chonghuan East Road, Shijie Town, Dongguan City, Guangdong Province, 523000 Patentee after: Guangdong Jijian Biotechnology Co.,Ltd. Address before: C02, Industrial Zone C, Chenwu Village, Shuinan, Shijie Town, Dongguan City, Guangdong Province, 523000 Patentee before: DONGGUAN JIJIAN BIOTECHNOLOGY Co.,Ltd. |