CN103992159B - A kind of full unit composite microbiological fertilizer and its preparation method and application - Google Patents
A kind of full unit composite microbiological fertilizer and its preparation method and application Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention provides one " full unit " composite microbiological fertilizer and its preparation method and application, belong to agriculture high production tech.By being CGMCC NO.5808 rich in bacillus amyloliquefaciens Bacillus amyloliquefaciens SQR 9(preserving number) biological organic fertilizer in add 14.8%(dry weight) ammonium sulfate make composite microbiological fertilizer total nutrient reach 10%, form then through disc granulation." full unit " composite microbiological fertilizer (granulation type) total nutrient: total nitrogen (TN)+full phosphorus (P2O5)+full potassium (K2O)=10%, function bacterium number >=2 × 10 after storing 6 months7CFU g‐1Fertilizer dry weight.New-type fertilizer has the synergistic function of the long-acting and bio-fertilizer of quick-acting, the fertilizer of chemical fertilizer concurrently, and growth-promoting is effective, production cost is low, use conveniently, can be applicable to cereal crops, vegetable etc..
Description
Technical field
The invention belongs to biological fertilizer field, relate to one " full unit " composite microbiological fertilizer and its preparation method and application.
Background technology
Some developed countries are the twenties in last century, China initially enters the agricultural modernization stage the sixties in last century, take the conventional agriculture modernization development pattern of " high investment, high production, high pollution ", level of agricultural production is made to increase substantially, but result also in the extreme consumption of resource, the heavy damage of ecological environment and pollution simultaneously.Along with the increase of the development of chemical fertilizer industry and agrochemical amount of application, the yield of crops has had very big growth.But chemical fertilizer is Agriculture Production make tremendous contribution while also result in substantial amounts of problem: (1) soil fertility decline (Chen Shiping. China's fertilizer structure modest proposal [J]. ten earth fertilizer, 1984 (4): 21～24.)；(2) soil physico-chemical property deteriorate (Wang Jianfei, Xing Suzhi. the negative effect of using in farmland chemical fertilizer and Preventing Countermeasures [J] thereof. agriculture environmental protection, 1998,17 (1): 40,--43)；(3) biological activity declines；(4) in soil, the balance of a great number of elements and trace element is destroyed；(5) crop failure, pest and disease damage serious (KevinG, Tiller, 1989；NishizauraNSMor, 1977)；(6) chemical fertilizer utilization ratio is low, pollution of ecological environment (Xia Rongji. soil and fertilizer rudimentary knowledge [M]. Beijing: agricultural publishing house, 1981.), aggravation greenhouse effect, polluted underground water (Liu little Lan, Li Shiqing. the nitrogen in soil and environment [J]. agricultural research in the arid areas, 1998,16 (4): 36～43.)；(7) affect agricultural product quality (Liu shares a bitter hatred for Hu Chengxiao, Deng Boer. the nitrogen level impact [J] on quality of vegetable. ten earth fertilizer, 1996 (3): 34～36.) etc..In China owing to long life hypertrophy amount is used with unreasonable, fertilizer takes effect slowly, use area and consumption wretched insufficiency and bio-bacterial manure efficiency is low, application small scale, without Fertilizer application features such as region specific aim, marketing difficulty are big, cause China's Soil structure to be seriously damaged, the content of organic matter sharply declines, structure of soil microbial community serious unbalance, thus causing agricultural output and quality significantly to decline and a series of distinct issues such as utilization rate of fertilizer reduction year by year.
Meanwhile, the fertilizer that the agricultural wastes such as substantial amounts of agricultural crop straw and feces of livestock and poultry are made, due to low fertilizer efficiency and added value, causes usable floor area and makes consumption seriously be obstructed, and causes ample resources waste and serious ecological environmental pollution.Therefore, can integrate that chemical fertilizer is quick-acting, fertilizer is long-acting and the new-type fertilizer production technology of bio-fertilizer potentiation by its Industry Promotion in the urgent need to developing, and then raising per unit area yield, complete the target to the newly-increased 100,000,000,000 jin of grain production capacity of the year two thousand twenty of country's establishment.
Summary of the invention
Present invention aim at using with unreasonable for long life hypertrophy amount, fertilizer takes effect slowly, use area and consumption wretched insufficiency and bio-bacterial manure efficiency is low, application small scale, without features such as region specific aim, marketing difficulty are big, it is simultaneous for the problem that powder fertilizer inconvenience mechanization is used, develops building performance " full unit " composite microbiological fertilizer (granulation type) of long-lasting and bio-bacterial manure integrating the quick-acting of inorganic fertilizer, fertilizer.
The preparation method that it is a further object of the present invention to provide " full unit " composite microbiological fertilizer.
It is yet another object of the invention to provide the application of " full unit " composite microbiological fertilizer.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of " full unit " composite microbiological fertilizer, prepares mainly through following steps:
1) adding mix homogeneously in biological organic fertilizer after ammonium sulfate pulverizing, excessively 18 mesh sieves and obtain mixed fertilizer, making mixed fertilizer total nutrient is 10%；
2) utilize granulating disc that mixed fertilizer is carried out pelletize, after granulating temperature less than 50 DEG C when the water content of mixed fertilizer grain is down to less than 20%, get product " full unit " composite microbiological fertilizer.
Wherein, described biological organic fertilizer is that 60% decomposed cow dung compost and 40% rapeseed cake compost carry out Homogeneous phase mixing, and bacillus amyloliquefaciens (Bacillusamyloliquefaciens) the SQR 9 fermentation liquid solid fermentation that inoculation preserving number is CGMCCNO.5808 forms；Described biological organic fertilizer is containing organic matter >=45%, total nutrient >=5%, water content≤30%, pH value 6.5 8.5.
Described bacillus amyloliquefaciens (Bacillusamyloliquefaciens) SQR 9 fermentation liquid is to be inoculated in PDA culture fluid by bacterial strain SQR9, carry out liquid fermentation production, the condition of fermenting and producing is: cultivation temperature scope 30～37 DEG C, mixing speed is 170～180 revs/min, fermentation middle and late stage forms spore, makes in fermentation liquid containing bacterium or spore amount >=1 × 109Individual/ml；PDA culture fluid compound method used is be cut into small pieces after peeling with 200g Rhizoma Solani tuber osi and be put in water and boil, and seethe with excitement post-cooked 30min, adds 20g common sucrose after filtering, be settled to 1000ml in filtrate, and pH value is adjusted to 7.0 7.6,121 DEG C of sterilizing 20min.
Described solid fermentation is to carry out solid fermentation in the cow dung compost mixture after described bacterial strain SQR9 fermentation liquid being inoculated rapeseed cake and becoming thoroughly decomposed, SOLID ORGANIC material per ton adds bacterial strain SQR9 fermentation liquid 40～60L, turning every day 1～2 time in solid fermentation process, make solid fermentation temperature less than 60 DEG C, terminating after fermenting 67 days, Promoting bacteria content can reach 2 × 108More than CFU/g fertilizer dry weight, finally temperature less than 60 DEG C when the water content of microbial organic fertilizer is evaporated to less than 30%, pulverize, cross 20 mesh sieves；This fertilizer quality of organic matter is than content >=45%, and organic nitrogen quality is 2 3% than content.
Described " full unit " composite microbiological fertilizer, with biological organic fertilizer dry weight for benchmark, the addition of ammonium sulfate is the 10～16% of biological organic fertilizer.
Described " full unit " composite microbiological fertilizer preserve 180 days after effective efficiency viable count >=2 × 107CFU/g fertilizer dry weight, total nitrogen content is 4 6%, and total npk nutrient is 10%, and finished particle is sized to diameter 1 2mm, water content≤20%.
Chemical fertilizer and biological organic fertilizer is utilized to produce the technique of " full unit " complex microorganism pelletize fertilizer, including:
1) rhizosphere growth-promoting bacillus amyloliquefaciens (Bacillusamyloliquefaciens) SQR9 that preserving number is CGMCCNO.5808 is inoculated in PDA culture fluid, carry out liquid fermentation production, the condition of fermenting and producing is: cultivation temperature scope 30～37 DEG C, mixing speed is 170～180 revs/min, fermentation middle and late stage forms spore, makes in fermentation liquid containing bacterium or spore amount >=1 × 109Individual/ml；PDA culture fluid compound method used is be cut into small pieces after peeling with 200g Rhizoma Solani tuber osi and be put in water and boil, and seethe with excitement post-cooked 30min, adds 20g common sucrose after filtering, be settled to 1000ml in filtrate, and pH value is adjusted to 7.0 7.6,121 DEG C of sterilizing 20min；
2) mass ratio is 60% cattle manure and 40% rapeseed cake compost carries out Homogeneous phase mixing；
3) the cow dung compost mixture after Promoting bacteria SQR9 fermentation liquid being inoculated rapeseed cake and becoming thoroughly decomposed carries out solid fermentation, SOLID ORGANIC material per ton adds growth-promoting bacterium solution 40～60L, turning every day 1～2 time in solid fermentation process, make solid fermentation temperature less than 60 DEG C, terminating after fermenting 67 days, Promoting bacteria content can reach 2 × 108More than CFU/g fertilizer dry weight, finally temperature less than 60 DEG C when the water content of microbial organic fertilizer is evaporated to less than 30%, this fertilizer quality of organic matter is than content >=45%, and organic nitrogen quality is 2 3% than content.Pulverize with disintegrating machine, cross 20 mesh sieves；
3) amount of the ammonium sulfate added is determined according to the concrete nutrient of above-mentioned biological organic fertilizer raw material so that it is after mixing, total nutrient is 10%.Ammonium sulfate need to pulverize after with sieve after biological organic fertilizer raw material mix homogeneously；
4) utilize granulating disc conventionally technique by mixed material pelletize, after granulating temperature less than 50 DEG C when the water content of microbial organic fertilizer is dried to less than 20%, packaging is dispatched from the factory and is finished product " full unit " complex microorganism pelletize fertilizer；Its organic nitrogen quality is 4 6% than content, and water content mass ratio is less than 20%.
Useful viable count >=2 × 10 in " full unit " composite microbiological fertilizer produced with above-mentioned Recipe7CFU/g(fertilizer dry weight), total nitrogen content is 4 6%, and total npk nutrient is 10%, the content of organic matter >=30%.This products material smashes and sieves, and finished particle is sized to diameter 1 2mm, and water content mass ratio is less than 20%.
" full unit " of the present invention composite microbiological fertilizer application in promoting crop growth and volume increase, it is preferable that the application in promoting Fructus Solani melongenae or tomato growth and volume increase.
Beneficial effect
Use " full unit " composite microbiological fertilizer that this invention produces integrate the quick-acting of inorganic fertilizer, fertilizer long-lasting and bio-bacterial manure building performance, fertilizer efficiency release is steadily, growth-promoting is effective, is suitable for the industrial crops planting area large area such as vegetable, fruit tree and uses.The present invention is for preserving the ecological environment, and the protection people's is healthy, and the added value improving agricultural product has great importance.
Product has the advantage that compared with product in the market
1) present invention is using the common fertilizer of decomposed cow dung and rape cake as composite fertilizer material, secondary solid fermentation rhizosphere growth-promoting BacillusamyloliquefaciensSQR9, have developed the good biological organic fertilizer of growth-promoting effect, adding a certain amount of ammonium sulfate after air-dry pulverizing makes its total nutrient reach 10%, utilize disc granulation technique pelletize, this formula and technique that the cost of " full unit " composite microbiological fertilizer can be greatly lowered." full unit " composite microbiological fertilizer is a kind of " type efficient, safe and environment-friendly " fertilizer.It combines bio-fertilizer, fertilizer, three kinds of fertilizer of inorganic fertilizer, makes the potentiation of bio-fertilizer, the quick-acting effects of short effect effect and the steady effect of fertilizer, long-acting and inorganic fertilizer be combined with each other, and anxious slow helps mutually, and nutrient is comprehensive.Both ensure that the indispensable nutritional labeling of plant growth, improve the soil again, growth-promoting, disease-resistant, improve the ecological environment, to produce high quality agricultural products, alleviating environmental pollution has great role.
2) in " full unit " composite microbiological fertilizer, the survival of microorganism is a critically important problem, the rhizosphere growth-promoting BacillusamyloliquefaciensSQR9 good salt tolerance that this patent uses, vitality is strong, and it has anti-yellowing cucurbit wilt, effect of wide spectrum growth-promoting, directly with a certain amount of ammonium sulfate mixing granulation, after storing 180 days after air-dry, meet living bacteria count >=2 × 107CFU/g(fertilizer dry weight).Direct mixed granulation process greatly reduces the production cost of " full unit " composite microbiological fertilizer.
3) " full unit " composite microbiological fertilizer after pelletize easily stores, and outward appearance is good, uses conveniently.
4) aminoacid resource abundant in fertilizer while can promote plant growth, the physiological regulation function that fertilizer collection microorganism is unique, and the high efficiency of inorganic fertilizer and the long-lasting of fertilizer, in one, reach the purpose of breeding combination and agricultural sustainable development.Result of the test shows, after using this product, can remarkably promote the growth of crop on vegetable sprout term, and effect of increasing production is extremely notable.
The present invention " full unit " composite microbiological fertilizer, has the activating soil of biological bacteria concurrently, converts the function of soil nutrient, the quick-acting functions of organic fertilizing soil, the feature of improvement soil and inorganic nutrients；The nutrition content of Nitrogen In Soils element, organic matter, titanium pigment, soluble potassium can be increased, increase beneficial microorganism population and quantity in soil, disease-resistant, drought-resistant ability and the metabolism of crop can be strengthened simultaneously；To improve crop yield, improve crop quality, increase peasant economy income suffer from positive effect.The research and development of novel all element compound fertilizer simultaneously can increase considerably the added value of fertilizer, thus promoting the development of such enterprise further, gos deep into resource organic waste protection environment." full unit " composite microbiological fertilizer, on conventional bacterial manure and inorganic fertilizer basis, increases Organic substance pretreatment technology or the special complex process when microorganism addition, strengthens organic proportion, highlights microorganism and organic effect.After incorporation of fertilizerin the soil, a large amount of probioticss are first impressions are strongest, it is suppressed that harmful bacteria is bred, and keep soil micro-ecosystem balance, and can strengthen biological activity of soil and biochemical activity, improve crop rhizosphere environment, play diseases prevention effect in strong sprout.That adds organic directly provides organic nutriment for crop, provides effective carrier for microorganism, and and microorganism cooperatively activating rhizosphere soil, improve soil physico-chemical property, make inorganic nutrients Stable Release in complex.Inorganic nutrients provides rational N, P, K three elements proportioning for different soils and Different Crop, adjusts the C/N ratio of complex, so as to be conducive to the flourish of microorganism and the performance of beneficial functions.And produce " full unit " composite microbiological fertilizer and can utilize the specific function of microorganism, agricultural organic waste being carried out harmless treatment, turns waste into wealth, make resource be fully used, environment improves and purifies.Be actually needed from current agricultural production, the factors such as specified value effect, fertilising custom, economic benefit and environmental conservation considers, it is the Main way of following Fertilizer development that this three is integrated, and can agricultural production be produced great pushing effect.
Accompanying drawing explanation
Fig. 1 " full unit " composite microbiological fertilizer granule
Fig. 2 " full unit " composite microbiological fertilizer storage period viable count change
Biomaterial preservation information
SQR9, Classification And Nomenclature is bacillus amyloliquefaciens Bacillusamyloliquefaciens, it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, preservation date is on February 27th, 2012, and preserving number is CGMCCNO.5808.
Detailed description of the invention
Embodiment 1
1, the common fertilizer of decomposed cow dung and rape cake provide
Cattle manure and rapeseed cake are bought by market and are obtained.
2, the production of function bacterium microbial inoculum
Rhizosphere growth-promoting bacillus amyloliquefaciens (Bacillusamyloliquefaciens) SQR9 that preserving number is CGMCCNO.5808 is inoculated in PDA culture fluid, carry out liquid fermentation production, the condition of fermenting and producing is: cultivation temperature scope 30～37 DEG C, mixing speed is 170～180 revs/min, fermentation middle and late stage forms spore, makes in fermentation liquid containing bacterium or spore amount >=1 × 109Individual/ml；PDA culture fluid compound method used is be cut into small pieces after peeling with 200g Rhizoma Solani tuber osi and be put in water and boil, and seethe with excitement post-cooked 30min, adds 20g common sucrose after filtering, be settled to 1000ml in filtrate, and pH value is adjusted to 7.0 7.6,121 DEG C of sterilizing 20min.
3, solid fermentation generates biological organic fertilizer
60% decomposed cow dung compost and 40% rapeseed cake are carried out Homogeneous phase mixing, Promoting bacteria SQR9 is accessed hybrid reactor, SOLID ORGANIC material per ton adds growth-promoting bacterium solution 50L and carries out solid fermentation, turning every day 1 time, make solid fermentation temperature less than 60 DEG C, terminate after fermenting 7 days.
In fermentation ends artifact fertilizer, Promoting bacteria SQR9 quantity reaches 5 × 108CFU/g(dry weight), quality of organic matter is than content >=45%, water content mass ratio 25 30%, and total nitrogen content is 2 more than 3%(90% is organic nitrogen), total npk nutrient is >=5%.
4, a certain amount of ammonium sulfate production of proportioning " full unit " composite microbiological fertilizer
Utilizing the method that organic fertilizer standard (NY525 2012) provides to measure total npk nutrient content in biological organic fertilizer, result is in Table 1.Biological organic fertilizer is air-dry to be smashed 20 mesh sieves and mixs homogeneously with the ammonium sulfate of pulverizing and obtain mixed fertilizer, and the addition of ammonium sulfate is the 14.8wt% of biological organic fertilizer dry weight, and mixed fertilizer total nutrient is 10%.Utilizing granulating disc pelletize, granule is air-dry less than 50 DEG C when makes water content mass ratio be low to moderate less than 20%.Sub-sieve, finished particle diameter is at 1 2mm, and packaging is finished product " full unit " composite microbiological fertilizer (Fig. 1).
Table 1 biological organic fertilizer raw material and composite microbiological fertilizer nutrient
Embodiment 2 warmhouse booth is tested
1. " full unit " composite microbiological fertilizer land for growing field crops effect on Fructus Solani melongenae
Set one process of three comparisons altogether, process " full unit " composite microbiological fertilizer (BOF) adding present invention development and impose appropriate chemical fertilizer, compare as (the CK that do not apply fertilizer1) and add the chemical fertilizer (CK of conventional amount used respectively2) and inactivation " full unit " composite microbiological fertilizer (AOF) impose appropriate chemical fertilizer, test kind is black galloping Fructus Solani melongenae, except not applying fertilizer except comparison, the nutrient such as other process.3 communities of each process, each zone leader 8m, wide 2.5 meters, area 20m2, 3kg(dry weight is used in every community) and " full unit " composite microbiological fertilizer, Routine Management.Results of regular determination Fructus Solani melongenae growth index.The different disposal impact on Fructus Solani melongenae biological character and structure of output, as shown in table 2, on plant height, " full unit " composite microbiological fertilizer (BOF) processes and is significantly better than other process (P=0.05), inactivation soil fertility quality (AOF) and chemical fertilizer (CK2) there was no significant difference between process but be significantly better than (the CK that do not apply fertilizer1) process.On stem is thick, BOF process (has maximum) and AOF process there was no significant difference, but is all remarkably higher than CK2And CK1.SPAD value, soluble protein and on soluble sugar content, difference that between all process, there are no significant.BOF process has maximum single fruit weight, is AOF, CK subsequently respectively2And CK1.The result of theoretical per mu yield aspect is BOF > AOF > CK2>CK1, and BOF process is significantly higher than CK2And CK1.Show that the application of NEW TYPE OF COMPOSITE microorganism pelletize fertilizer (BOF) can significantly improve biological character and the structure of output of crop.
Different disposal is on the impact of yield in Table 3, and " full unit " composite microbiological fertilizer processes than the volume increase 860kg/ mu that do not apply fertilizer, and amount of increase in production reaches 31.2%；Inactivation treatment is than the volume increase 653kg/ mu that do not apply fertilizer, and amount of increase in production reaches 23.7%.Meanwhile, " full unit " complex microorganism pelletize soil fertility quality increases production 273kg/ mu than Single fertilizer, and amount of increase in production reaches 8.17%；Inactivation treatment increases production 66kg/ mu than Single fertilizer, and amount of increase in production reaches 1.97%." full unit " composite microbiological fertilizer processes and increases production 207kg/ mu than inactivation treatment, and amount of increase in production reaches 6.07%.
Yield result is carried out variance analysis discovery (table 4, table 5), F between process > F0.01>F0.05；And F < F between repeating0.05<F0.01, diversity notable (P=0.01) between namely processing, in repeating, under P=0.05 and P=0.01, difference is all not notable.Meanwhile, the diversity between comparison process and between repeating finds, F > F0.01>F0.05, say, that under P=0.05 and P=0.01, the variation between process is significant more than the variation between repeating, and different disposal has different effect for eggplant yield.Between yield is processed, diversity significance analysis finds: " full unit " composite microbiological fertilizer, compared with chemical fertilizer and comparison of not applying fertilizer, has significant effect of increasing production (P=0.05), and yield processes higher than inactivation compound fertilizer simultaneously.
Table 2 " full unit " composite microbiological fertilizer impact on Fructus Solani melongenae biological character and structure of output
The impact on eggplant yield result of table 3 different disposal
Table 4 yield result analysis of variance table
The Notable difference test (LSR method) of table 5 yield
5.2 " full unit " composite microbiological fertilizer land for growing field crops effect on Fructus Lycopersici esculenti
EXPERIMENTAL DESIGN and the same Fructus Solani melongenae of fertilizer applications, test kind is century red hat.On the impact of tomato biological character and structure of output as shown in table 6 and table 7, on plant height, " full unit " composite microbiological fertilizer (BOF) processes and is significantly better than chemical fertilizer comparison (CK different disposal2), not fertilizer treatment (CK1) and inactivation fertilizer process (AOF) (P=0.05).On stem is thick, BOF process is significantly better than chemical fertilizer comparison and not fertilizer treatment.In SPAD value, VC content and sugar-acid ratio, difference that between all process, there are no significant.BOF process has maximum single fruit weight, is AOF, CK subsequently respectively2And CK1.The result of theoretical per mu yield aspect is BOF > AOF > CK2>CK1, and BOF process is significantly higher than AOF, CK2And CK1.Show that the application of " full unit " composite microbiological fertilizer (BOF) can significantly improve biological character and the structure of output of crop.
Different disposal is on the impact of yield in Table 8, and on the impact of yield as shown in Table 3, " full unit " compound microbial manure is than the volume increase 1394.03kg/ mu that do not apply fertilizer, and amount of increase in production reaches 37.73% for different disposal；Increasing production 473.57kg/ mu than Single fertilizer, amount of increase in production reaches 10.26%；Than inactivation soil fertility quality volume increase 386.86kg/ mu, amount of increase in production reaches 8.23%.
Output statistics analyzes result in Table 9 and 10, F between process > F0.01>F0.05, F < F between repetition0.05<F0.01, diversity notable (P=0.01) between namely processing, in repeating, under P=0.05 and P=0.01, difference is all not notable.Meanwhile, the diversity between comparison process and between repeating finds, F > F0.01>F0.05, say, that under P=0.05 and P=0.01, the variation between process is noticeably greater than the variation between repetition, and different disposal is have different effect for tomato yield.Between yield is processed, diversity significance analysis finds: " full unit " composite microbiological fertilizer all has significant difference with other process under P=5%；And inactivation treatment, chemical fertilizer all do not have significant difference at 5% time between processing, but compare with the process do not applied fertilizer and be respectively provided with significant difference.
Table 6 " full unit " composite microbiological fertilizer impact on tomato biological character and structure of output
Table 7 " full unit " composite microbiological fertilizer impact on tomato yield structure
The impact on tomato yield result of table 8 different disposal
Table 9 yield result analysis of variance table
The Notable difference test (LSR method) of table 10 yield
Mixing granulation test and storage experiments show that, the formula of this research and technique ensure that function bacterium number of viable.With granulating disc by the mixed material pelletize after sieving, controlling material amount of water and granulation time, the fertilizer after pelletize is air-dry to water content mass ratio less than 20%.A part is for land for growing field crops fertilising, and a part is placed in plastic bag and seals keeps in Dark Place, and counts once with method of dilution butteron on plate at set intervals, and result is as shown in Figure 2.
Warmhouse booth result of the test entirety shows, " full unit " composite microbiological fertilizer (granulation type) use the yield that can be effectively increased facility Fructus Solani melongenae and Fructus Lycopersici esculenti, compare with inactivate fertilizer, chemical fertilizer compares and does not apply fertilizer comparison, Fructus Solani melongenae has increased production 6.07%, 8.17% and 31.2% respectively, and Fructus Lycopersici esculenti has increased production 8.23%, 10.26% and 37.73% respectively.Show potentiation and the one of the quick-acting and functional microorganism of long-acting, the chemical fertilizer of this patent product collection fertilizer.Therefore, this patent is researched and developed " full unit " complex microorganism granulation fertilizer material formula and technique have very strong practicality.
Claims (6)
1. one kind " full unit " composite microbiological fertilizer, it is characterised in that prepare mainly through following steps:
1) after ammonium sulfate pulverizing, excessively 18 mesh sieves, adding mix homogeneously in biological organic fertilizer and obtain mixed fertilizer, making mixed fertilizer total nutrient is 10%；Wherein, with biological organic fertilizer dry weight for benchmark, the addition of ammonium sulfate is the 10 ~ 16% of biological organic fertilizer；Described biological organic fertilizer is first 60% decomposed cow dung compost and 40% rapeseed cake to be carried out Homogeneous phase mixing；By growth-promoting bacillus amyloliquefaciens that preserving number is CGMCCNO.5808 (Bacillusamyloliquefaciens) SQR-9 fermentation liquid inoculates and carry out solid fermentation in rapeseed cake and decomposed cow dung compost mixture and form；Described biological organic fertilizer is containing organic matter >=45%, total nutrient >=5%, water content≤30%, pH value 6.5-8.5；
2) utilize granulating disc that mixed fertilizer is carried out pelletize, after granulating temperature less than 50 DEG C when the water content of mixed fertilizer grain is down to less than 20%, get product " full unit " composite microbiological fertilizer；Described " full unit " composite microbiological fertilizer preserve 180 days after effective efficiency viable count >=2 × 107CFU/g fertilizer dry weight, total nitrogen content is 4-6%, and total npk nutrient is 10%, and finished particle is sized to diameter 1-2mm, water content≤20%.
2. " full unit " composite microbiological fertilizer according to claim 1, it is characterized in that described bacillus amyloliquefaciens SQR-9 fermentation liquid is to be inoculated in PDA culture fluid by bacillus amyloliquefaciens SQR9, carry out liquid fermentation production, the condition of fermenting and producing is: cultivation temperature scope 30～37 DEG C, mixing speed is 170～180 revs/min, fermentation middle and late stage forms spore, makes in fermentation liquid containing bacterium or spore amount >=1 × 109Individual/ml.
3. " full unit " composite microbiological fertilizer according to claim 2, it is characterized in that described solid fermentation is to inoculate described SQR9 fermentation liquid in rapeseed cake and decomposed cow dung compost mixture to carry out solid fermentation, SOLID ORGANIC material per ton adds SQR9 fermentation liquid 40 ~ 60L, turning every day 1 ~ 2 time in solid fermentation process, make solid fermentation temperature less than 60 DEG C, terminating after fermenting 6-7 days, SQR9 content reaches 2 × 108More than CFU/g fertilizer dry weight, finally temperature less than 60 DEG C when the water content of microbial organic fertilizer is evaporated to less than 30%, pulverize, cross 20 mesh sieves；Quality of organic matter content >=45% in described biological organic fertilizer, organic nitrogen mass content is 2-3%.
4. the preparation method of " full unit " composite microbiological fertilizer described in claim 1, it is characterised in that comprise the steps of
1) the bacillus amyloliquefaciens SQR9 that preserving number is CGMCCNO.5808 is inoculated in PDA culture fluid, carry out liquid fermentation production, fermentation condition is: cultivation temperature 30～37 DEG C, mixing speed 170～180 revs/min, fermentation middle and late stage forms spore, makes in fermentation liquid containing bacterium or spore amount >=1 × 109Individual/ml；
2) 60% cattle manure and 40% rapeseed cake compost are carried out Homogeneous phase mixing；
3) bacterial strain SQR9 fermentation liquid step 1) prepared is inoculated in rapeseed cake and decomposed cow dung compost mixture and is carried out solid fermentation, SOLID ORGANIC material per ton adds bacterial strain SQR9 fermentation liquid 40 ~ 60L, turning every day 1 ~ 2 time in solid fermentation process, make solid fermentation temperature less than 60 DEG C, terminating after fermenting 6-7 days, SQR9 content reaches 2 × 108More than CFU/g fertilizer dry weight, finally temperature less than 60 DEG C when the water content of microbial organic fertilizer is evaporated to less than 30%, pulverize, cross 20 mesh sieves；This fertilizer quality of organic matter is than content >=45%, and total nitrogen content is 2-3%；
4) determine the amount of the ammonium sulfate added according to the concrete nutrient of above-mentioned biological organic fertilizer, ammonium sulfate pulverize after with sieve after biological organic fertilizer raw material mix homogeneously make it mix after total nutrient be 10% obtain mixed fertilizer；
5) utilize granulating disc will obtain mixed fertilizer pelletize, after granulating temperature less than 50 DEG C when the water content of microbial organic fertilizer is dried to less than 20%, " full unit " composite microbiological fertilizer.
5. " full unit " composite microbiological fertilizer application in promoting crop growth and volume increase according to any one of claim 1 ~ 3.
6. application according to claim 5, it is characterised in that described " full unit " composite microbiological fertilizer application in promoting Fructus Solani melongenae or tomato growth and volume increase.
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| CN107686406A (en) * | 2017-09-20 | 2018-02-13 | 南京农业大学 | A kind of special trichoderma of garlic first bio-organic fertilizer and its preparation method and application entirely |
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| CN102617203B (en) * | 2012-04-16 | 2014-05-07 | 南京农业大学 | Method for producing microbial organic fertilizer by utilizing poultry feathers and product thereof |
| CN102898197A (en) * | 2012-11-01 | 2013-01-30 | 南京农业大学 | Technology for producing growth promoting bioorganic fertilizer by using algae mud as additive and product |
| CN102924141A (en) * | 2012-11-01 | 2013-02-13 | 南京农业大学 | Process for utilizing three abandoned proteins and thoroughly-decomposed cow dung to produce growth-promoting bio-organic fertilizer and product thereof |
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