A kind of PCR plate injection moulding method
Technical field:
The present invention relates to PCR plate injection moulding technical field, relate in particular to a kind of PCR plate injection moulding method.
Background technology:
At present, in molecular biology research and medical clinic applications process, PCR reaction technology is indispensable.PCR reaction is PCR (polymerase chain reaction, PCR), is a kind of method of specific gene of rapid amplifying in vitro or DNA sequence dna, therefore be called again the amplification in vitro method of gene.At two Oligonucleolide primers of DNA segment both sides He Yuqi both sides complementation to be amplified, after several circulations of sex change, annealing and extension, DNA cloning 2 square time doubly.For amplification in batches, in PCR reaction, commonly use PCR reaction plate, see shown in accompanying drawing 1~3, PCR reaction plate include connecting plate 1 ' and a plurality of reaction tubes 2 ', PCR reaction plate is generally 8 holes, 12 holes, 96 holes or 384 holes, being corresponding reaction tube 2 ' be 8,12,96 or 384, is specially for batch reaction designs, for PCR instrument and sequenator.
Existing PCR reaction plate is when injection mo(u)lding, generally PCR reaction plate is inverted and is carried out injection mo(u)lding, injection forming mold includes patrix, the counterdie matching with patrix, counterdie is arranged on patrix lower surface, between patrix and counterdie, form PCR template chamber, PCR template chamber have connecting plate die cavity 5 ' and a plurality of reaction tube die cavity 6 that is upside down in connecting plate die cavity 5 ' upper end ', while entering glue, general be all each reaction tube die cavity 6 ' top arrange respectively a glue-feeder 7 ', by each glue-feeder 7 ' while, to PCR template chamber, inject raw material, but each reaction tube bottom of the PCR plate of producing like this can be formed with the mouth of a river, reaction tube lower surface is rough, irregular, size is unstable, affect the effect that penetrates of light, be unfavorable for the example reaction situation in observing response pipe, impact detects effect, and the glue-feeder that injection forming mold needs is many, mould structure is complicated, production efficiency is low, production cost is high, waste raw material.
Summary of the invention:
Object of the present invention is exactly the deficiency existing for prior art and a kind of PCR plate injection moulding method that can improve product surface quality and performance, raising injection moulding efficiency is provided.
To achieve these goals, the technical solution used in the present invention is:
A PCR plate injection moulding method, comprises the following steps:
A, preparation PCR sheet frame, make PCR sheet frame form a plurality of hole positions corresponding with reaction tube;
B, in mould, form PCR template chamber, the PCR sheet frame die cavity, connecting plate die cavity, a plurality of reaction tube die cavity that is upside down in connecting plate die cavity upper end that PCR template chamber are had mate with PCR sheet frame, a plurality of reaction tube die cavities are corresponding one by one with position, a plurality of hole, and the PCR sheet frame making is placed in to PCR sheet frame die cavity;
C, the plastic material of molten condition is injected to PCR template chamber by connecting plate die cavity, make plastic material be full of connecting plate die cavity and reaction tube die cavity and be combined into one and form PCR plate with PCR sheet frame.
PCR sheet frame in steps A is injection molded by PC material.
Plastic material in step C is PP material.
Before carrying out described step C, first utilize air compression system to produce negative inspiratory pressure state, by the top of each reaction tube die cavity, by the air sucking-off in PCR template chamber, make formation negative pressure PCR template chamber in.
In mould, offer in advance the negative pressure line being communicated with extraneous air compressibility, negative pressure line be communicated with the top of each reaction tube die cavity respectively, by negative pressure line by the air sucking-off in PCR template chamber.
In described step C, in mould, offer in advance a plurality of glue-feeders, each glue-feeder is communicated with connecting plate die cavity respectively, by each glue-feeder, to PCR template chamber, inject plastic material simultaneously.
Described a plurality of reaction tube die cavity is divided into a plurality of groups, between each reaction tube die cavity in every group, by a connecting plate die cavity, is communicated with, and each connecting plate die cavity is corresponding to be communicated with a glue-feeder.
Described a plurality of reaction tube die cavity is matrix distribution, and every 4 adjacent reaction tube die cavities are one group,
Described connecting plate die cavity is positioned at the upper end of PCR sheet frame die cavity.
Beneficial effect of the present invention is: the present invention includes following steps: A, preparation PCR sheet frame, make PCR sheet frame form a plurality of hole positions corresponding with reaction tube, B, in mould, form PCR template chamber, make PCR template chamber there is PCR sheet frame die cavity, connecting plate die cavity, a plurality of reaction tube die cavity, and the PCR sheet frame making is placed in to PCR sheet frame die cavity, C, the plastic material of molten condition is injected to PCR template chamber by connecting plate die cavity, make plastic material be full of connecting plate die cavity and reaction tube die cavity and be combined into one and form PCR plate with PCR sheet frame, the mouth of a river of the PCR plate making is positioned at the inner side of connecting plate, and on reaction tube, there is no the mouth of a river, improve product surface quality and performance, reaction tube smooth surface is neat, dimensionally stable, translucent effect is good, example reaction situation in observing response pipe well, improve product and detect effect, and the required glue-feeder of mould is fewer, simplify mould structure, production efficiency is high, water port of product is also few, production cost is low, conservation.
Accompanying drawing explanation:
Fig. 1 is the structural representation of existing PCR plate.
Fig. 2 is the injection mo(u)lding schematic diagram of existing PCR plate.
Fig. 3 is the glue-feeder distribution schematic diagram of existing PCR plate.
Fig. 4 is the structural representation of injection forming mold of the present invention.
Fig. 5 is the structural representation in PCR template of the present invention chamber.
Fig. 6 is the injection mo(u)lding schematic diagram of PCR plate of the present invention.
Fig. 7 is the glue-feeder distribution schematic diagram that utilizes the PCR plate that the present invention makes.
Fig. 8 is the structural representation that utilizes the PCR plate that the present invention makes.
The specific embodiment:
Below in conjunction with accompanying drawing and preferred embodiment, the invention will be further described, see shown in Fig. 4~8, PCR plate includes PCR sheet frame 1, a plurality of connecting plate 2 and a plurality of reaction tube 3, a plurality of reaction tubes 3 are matrix distribution, every 4 adjacent reaction tubes 3 are one group, between 4 reaction tubes 3 in each group, by a connecting plate 2, connect, PCR sheet frame 1 is made by PC material, and connecting plate 2 and reaction tube 3 are made by PP material.Certainly all reaction tubes 3 also can only connect by a connecting plate 2.
The injection molding forming method of above-mentioned PCR plate, comprises the following steps:
One, preparation PCR sheet frame 1, makes PCR sheet frame 1 form a plurality of hole positions corresponding with reaction tube 3.
Two, in mould 4, form PCR template chamber, PCR template chamber the is had PCR sheet frame die cavity 41 mating with PCR sheet frame 1, the connecting plate die cavity 42 that is positioned at PCR sheet frame die cavity 41 upper ends, a plurality of reaction tube die cavity 43 that is upside down in connecting plate die cavity 42 upper ends, a plurality of reaction tube die cavities 43 and the corresponding connection one by one of position, a plurality of hole, and the PCR sheet frame 1 making is placed in to PCR sheet frame die cavity 41; Certainly, connecting plate die cavity 42 also can be positioned at PCR sheet frame die cavity 41 lower ends.
Three, utilize air compression system to produce negative inspiratory pressure state, by the top of each reaction tube die cavity 43, by the air sucking-off in PCR template chamber, make formation negative pressure PCR template chamber in.
Wherein, in mould 4, offer in advance the negative pressure line 5 being communicated with extraneous air compressibility, negative pressure line 5 be communicated with the top of each reaction tube die cavity 43 respectively, by negative pressure line 5 by the air sucking-off in PCR template chamber.
Four, the plastic material of molten condition is injected to PCR template chamber by connecting plate die cavity 42 upper ends, reaction tube die cavity 43 under negative pressure state can attract raw material to rise and enter in reaction tube die cavity 43 in connecting plate die cavity 42, make plastic material be full of fast connecting plate die cavity 42 and reaction tube die cavity 43 and be combined into one and form PCR plate with PCR sheet frame 1, injection moulding efficiency is high, and avoid oxygen in PCR template chamber to react and make plastic material burn with plastic material generation, improve performance and the surface quality of plastic cement products, can injection molding more accurate, the product of the less and thin wall type of size, in addition, the present invention also can utilize air compression system malleation blowing method that product is blown out, and prevents that product is bonded on patrix, prevents deformation of products and damage, and the demoulding is easy.
Wherein, in mould 4, offer in advance a plurality of glue-feeders 6, each glue-feeder 6 is distributed in respectively in the space between adjacent a plurality of reaction tube die cavity 43, the bottom of each glue-feeder 6 is communicated with connecting plate die cavity 42 upper ends respectively, by each glue-feeder 6, to PCR template chamber, injects plastic material simultaneously.A plurality of reaction tube die cavities 43 are divided into a plurality of groups, between each reaction tube die cavity 43 in every group, by a connecting plate die cavity 42, be communicated with, each connecting plate die cavity 42 correspondence is communicated with a glue-feeder 6, by each glue-feeder 6, injects plastic material to PCR template chamber simultaneously, conveniently enters glue simultaneously.In the present embodiment, a plurality of reaction tube die cavities 43 are matrix distribution, and every 4 adjacent reaction tube die cavities 43 are one group.
PCR sheet frame 1 is injection molded by PC material, and the plastic material of connecting plate 2 and reaction tube 3 is PP material, makes the PCR plate making have good stability, and heat conductivility is good, repeatedly heats indeformable advantage.
Utilize the mouth of a river of the PCR plate that the inventive method makes to be positioned at the inner side of connecting plate 2, and on reaction tube 3, there is no the mouth of a river, improve product surface quality and performance, reaction tube 3 smooth surfaces are neat, dimensionally stable, and translucent effect is good, example reaction situation in observing response pipe 3 well, improve product and detect effect, and the required glue-feeder 6 of mould is fewer, simplify mould structure, production efficiency is high, water port of product is also few, and production cost is low, conservation.
Certainly, the above is only preferred embodiment of the present invention, and the equivalence of doing according to structure, feature and principle described in patent claim of the present invention therefore all changes or modifies, and is included in patent claim of the present invention.